LOCUS       NZ_CP117739             3817 bp    DNA     circular CON 25-MAR-2024
DEFINITION  Klebsiella grimontii strain 2022CK-00781 plasmid unnamed9, complete
            sequence.
ACCESSION   NZ_CP117739
VERSION     NZ_CP117739.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32733981
            Assembly: GCF_028622675.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella grimontii
  ORGANISM  Klebsiella grimontii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2023) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease Submission Group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 61.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028622675.1-RS_2024_03_25
            Annotation Date                   :: 03/25/2024 02:14:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,580
            CDSs (total)                      :: 6,462
            Genes (coding)                    :: 6,291
            CDSs (with protein)               :: 6,291
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 171
            CDSs (without protein)            :: 171
            Pseudo Genes (ambiguous residues) :: 0 of 171
            Pseudo Genes (frameshifted)       :: 62 of 171
            Pseudo Genes (incomplete)         :: 127 of 171
            Pseudo Genes (internal stop)      :: 25 of 171
            Pseudo Genes (multiple problems)  :: 35 of 171
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117739.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Klebsiella grimontii"
                     /mol_type="genomic DNA"
                     /strain="2022CK-00781"
                     /isolation_source="skin"
                     /host="Homo sapiens"
                     /db_xref="taxon:2058152"
                     /plasmid="unnamed9"
                     /geo_loc_name="USA"
                     /collection_date="2022-11-28"
     gene            complement(1210..1509)
                     /locus_tag="PTC90_RS32820"
                     /old_locus_tag="PTC90_32815"
     CDS             complement(1210..1509)
                     /locus_tag="PTC90_RS32820"
                     /old_locus_tag="PTC90_32815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="WP_024262786.1"
                     /translation="MYAIAFDLDQDMLEQTYHVPSYQNAYGDIKKALEAHGFTRQQGSV
                     YFGDVSKVDAVSCVLAVMDLAKKNPWFAPSVQDIRMLRIEENNDLMPAVRKAVE"
                     /name="virulence factor"
     gene            complement(1521..1769)
                     /locus_tag="PTC90_RS32825"
                     /old_locus_tag="PTC90_32820"
     CDS             complement(1521..1769)
                     /locus_tag="PTC90_RS32825"
                     /old_locus_tag="PTC90_32820"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023063530.1"
                     /translation="MGRKKNQLGTQIHQLKKSNDKIFSALASTASRLDAVERVQADADM
                     RVRNLEIKMKSMSGAKNKDIAVEYDLSEGRVSQIINQ"
                     /name="hypothetical protein"
     gene            complement(2059..2274)
                     /locus_tag="PTC90_RS32830"
                     /old_locus_tag="PTC90_32825"
     CDS             complement(2059..2274)
                     /locus_tag="PTC90_RS32830"
                     /old_locus_tag="PTC90_32825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_097739831.1"
                     /translation="MTDLEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSNS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2281..2778)
                     /locus_tag="PTC90_RS32835"
                     /old_locus_tag="PTC90_32830"
     CDS             complement(2281..2778)
                     /locus_tag="PTC90_RS32835"
                     /old_locus_tag="PTC90_32830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_142375681.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEQSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTSIREQKDALEKLNARTWGVRYQEDNQGRFLVLPEGVKADTNWTFDNGRKNGIRLV
                     QE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2813..3466)
                     /locus_tag="PTC90_RS32840"
                     /old_locus_tag="PTC90_32835"
                     /pseudo=""
     CDS             complement(<2813..3466)
                     /locus_tag="PTC90_RS32840"
                     /old_locus_tag="PTC90_32835"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3456..3779)
                     /locus_tag="PTC90_RS32845"
                     /old_locus_tag="PTC90_32840"
     CDS             complement(3456..3779)
                     /locus_tag="PTC90_RS32845"
                     /old_locus_tag="PTC90_32840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_170989381.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGNGHDRVHVVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(72..251)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.339"
                     /coverage="83.0"
                     /evalue="7.539999999999999e-48"
     Typing          227..716
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.697"
                     /coverage="94.0"
                     /evalue="2.71e-175"
     Typing          complement(1962..3466)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.625"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gccgggacgg gcgggaaatc ttttcccggg cggcgcggtg ttggacaagc cgcaggcgcg
       61 tcagtggctt ttagcgggtt tcggggcgca gccctgaacc agtcacgtag cgctagcgga
      121 gtgtatactg gcttaatatg gtaaatcgaa gtgggaattc aggaaagtgc ttcatgtggc
      181 aggggaaaaa tgcggatgcg gtgcgtaagt gcagaatgtc atgatcggtg aaatgacgcc
      241 tcctcgctca ctcggtcgct gcgctcctgc cgtgagactg tggcgggcgt tacaagcaca
      301 cgaatggcga gataaagaga ctgcgctcaa atcagaaagg gcgaaaaaaa ggatgcgtcg
      361 tggccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat
      421 cagtggcggc gaaacccgac aggactataa agatcccagg cgtttccccc tggtagctcc
      481 ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcactccgct gttatggccg
      541 cggtttctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
      601 tgtatgcacg aaccccccgt tcagtccgac tgctgcgcct tatccggtaa ctatcgtctt
      661 gagtccaatc cgaaaaaggc acgacaaagc gccactggca gcagccattg gtaacttgtg
      721 tacgagagat atttagagag ttcttgaagt ggtggcctga ctgcggctac actgaaagga
      781 cagtatttgg tatctgtggt ctcgtacacc cagttaccta cggaataaag agttggtagc
      841 tctgaattcc ggcaaactaa ccaccgttgg tagcggtggt ttttttgttt acggcttagg
      901 agattacgct cagaaacgaa tgatctcaat aacaagtgtt atttgatctt caacttgaag
      961 attttctagc tcttctctct gcattatctt tagcacgaca cgatttacac caactctgat
     1021 ttctcatcgc gtcatccata tttctgaaac caaacaatgc atataactct tgaaatgagt
     1081 tagcagttac tgggaattga gcgcactcat attttgcaca tggacaaaca tgtggaagac
     1141 ttatttcacc agatgacatc tcactcataa atacccccag acatattatt aatgtgcaaa
     1201 aattaaagtt tattctactg ccttacgaac tgctggcata agatcgttat tttcctcaat
     1261 cctcaacatc ctaatatctt gaacagatgg agcgaaccac ggattttttt ttgccaaatc
     1321 cattacagcc aaaacacaag aaacagcatc gactttagat acatccccaa aatatacact
     1381 tccttgctgt cttgtgaagc catgtgcttc tagagccttc ttgatgtctc cataagcatt
     1441 ctgataacta ggtacatggt atgtttgctc taacatatcc tgatcaagat caaacgctat
     1501 agcgtacata ataattattc ctactgattt ataatttgag agacacgacc ttcagacaga
     1561 tcatactcaa cggcaatatc tttatttttg gcaccagaca tagacttcat ctttatctca
     1621 agattcctaa ctctcatatc ggcatcagct tgaactcgct caacagcatc aagacgcgat
     1681 gctgtacttg ctaaagctga aaagatcttg tcgttacttt tctttaactg atgaatctga
     1741 gttcccagtt ggtttttttt acgccccatc tttgcaccat taagattgct atagttgcca
     1801 acgacaatac aacaacaaac ataaatttca acaaaaaagc acctcaatta aagaacaaaa
     1861 aataaaaaca agcaaaatca acaagataga aaaaaaaagt ttagcgagca tgtattttat
     1921 ttagcttaaa agcaaattaa ccaatagata cagtcactta aagtgttggt ccatatctag
     1981 ctctcggtgc tttttctagc tccagtattt tctggtccct ctggatccgt tcatgctgct
     2041 ggcgctgttc gattgtattc aactgttcga caaccggcgc agctgctcgc tcaaactctg
     2101 cacctgcctg ctcaagcccg tgacgcgctc gctcagcgcc gcgttctccc gttgcataag
     2161 tccagacatc gtccgccatt ccgcgaaggc gttctcccac tcgtccagcc ttttcgagta
     2221 gtcctgttgt agctgctcta atgcgctcaa taactgtttt tccagatccg tcatgttgct
     2281 ctactcctgt accagtctga tgccattctt cctgccattg tcgaacgtcc agtttgtgtc
     2341 cgctttcacg ccctccggca gcaccagaaa gcgcccctgg ttgtcttcct gatacctcac
     2401 gccccacgtc cgggcgttca gcttctccag cgcgtctttc tgctcccgga tgctcgtgta
     2461 gttgtcgagt atctgctgcc cctgccacca cagaatgccc gcgctcgatg cgatcagcag
     2521 cgcggagacc aggacgatgg tcagccacgt ctggctgacc atgcgcagca tcccttttgt
     2581 gcgctggctc atggctgagg acagcttccg gtcgtggtcg aggatggcgg cgctgattct
     2641 cttctcgctt tcgctcagtt ccgctctgac agactgctcg tgctcgctga atgcggcttt
     2701 cagcatctcg ccggtactct gctgctgcgc tttcgatttc tgctctaagt ccttcgccag
     2761 cgttaaaaga ctgttcatag atggctccct tcagtcggat atttcgcccc ccgtccgggt
     2821 cggcaatgct gatgctgctt ttcgtggtgc gcaccacctc aaaacccgcg ctttccagcg
     2881 cctcagtgac gtcctgccgg gttttgagct ccccggacga ggctaaggcc agtaatcccc
     2941 gtgtaatcgt ctgagcagcg tccagtttcg cctccggcag cgcggacgac gtgaccagcg
     3001 cccgccggtt ttccggcccg ttcgggtcgt gcagccccag cctgccgttc actatggtct
     3061 gccaggcatc aatgcgcgga cgatcggcgc ggtcgtaata cggctgtagc cgcttgcctg
     3121 tgagcagctc cgtgttcggg atcaggaagt tcagctccag ccgcccctta tcctggtgct
     3181 caacccacag cacgctgtac tggtctttat cgagtccggg catcagaaca cgctcaaagc
     3241 tcgccatcag cttttcgcgc tggccgggcg gtaaatcctt ttcggcaaag gacagaacgc
     3301 cggatgtgta ctttttggcg tagggcgagg cgtcgataag ctcccggact tcctccggct
     3361 tcccctgcag gacagtggcg ccgtcgcgct gccgatcttt ccccagcaga taatccaccg
     3421 gaccggcgcc accgccacgt cctcgcgggt gaaacttaac gatcatcgtc tgtccccttt
     3481 tccagcacgg cgtgcctcag ccgctcgagt ccggcatcga tggccatcag cgcggcgacg
     3541 acatgcacgc ggtcgtgccc gttaccgcca ccggcgttaa cctgccgggc aatctggttg
     3601 aggttattcc ccatgcccgc aagctggcga agcagcgccg gcgagatgga gggaagtttg
     3661 ccggcacggg cgggcttttc gtccaggcac gtctgccgca tccaggccgc gagttgctta
     3721 ccctcgcagc gctccagcaa acgccggtgc tcatcctcag tcacccacat cgtgagcatt
     3781 ttgctgcgtt tgtctgccaa tccggcctcc tgataac
//
