LOCUS       NZ_CP117674            33310 bp    DNA     circular CON 25-MAR-2024
DEFINITION  Escherichia coli strain KW21 plasmid pKW21-2, complete sequence.
ACCESSION   NZ_CP117674
VERSION     NZ_CP117674.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33198549
            Assembly: GCF_028622955.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33310)
  AUTHORS   Jiang,H. and Zhang,L.
  TITLE     Complete genome sequence of E. coli KW21
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 33310)
  AUTHORS   Jiang,H. and Zhang,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2023) Colleage of Veterinary Medicine, South China
            Agricultural University, 483 Wushan Road, Guangzhou 510642, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 21-OCT-2020
            Assembly Method                   :: HGAP v. 4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 552.0x
            Sequencing Technology             :: PacBio; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028622955.1-RS_2024_03_25
            Annotation Date                   :: 03/25/2024 01:47:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,979
            CDSs (total)                      :: 4,858
            Genes (coding)                    :: 4,667
            CDSs (with protein)               :: 4,667
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 191
            CDSs (without protein)            :: 191
            Pseudo Genes (ambiguous residues) :: 0 of 191
            Pseudo Genes (frameshifted)       :: 78 of 191
            Pseudo Genes (incomplete)         :: 117 of 191
            Pseudo Genes (internal stop)      :: 26 of 191
            Pseudo Genes (multiple problems)  :: 30 of 191
            Pseudo Genes (short protein)      :: 3 of 191
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117674.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..33310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="KW21"
                     /isolation_source="water"
                     /db_xref="taxon:562"
                     /plasmid="pKW21-2"
                     /geo_loc_name="China: Guangzhou"
                     /collection_date="2018-11-02"
     gene            join(32296..33310,1..14)
                     /gene="virB11"
                     /locus_tag="PSR34_RS24670"
                     /old_locus_tag="PSR34_24670"
     CDS             join(32296..33310,1..14)
                     /gene="virB11"
                     /locus_tag="PSR34_RS24670"
                     /old_locus_tag="PSR34_24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401678.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_000017226.1"
                     /translation="MSGVIPVSISNKSLDFYKNQVFQSFLDIEGLTEIAVNRPGEIWTE
                     INGDWTFHNNDSVTFDFCKRFSHTLASFRGDEIGETKPLLSATLESGERVQVVFPPACE
                     RNTISITIRKPSTRQITHKEYVANGFYDYVQSGKKHRTYDDELLDLFRARNIAAFMELA
                     VAAGKTIVFAGATGSGKTTYMKSLIDFIPLNTRLITIEDAEEIKFFIHKNYVHLFYPSE
                     SGSDSGSIITAAKLIKSCLRMKPDRILLAEIKGGDAWDFVKVAGSGHSGSMTSIHAASA
                     KDAIIQMVTKCYQNRECQNLPFDVLRKIIMDSIDIVVHVGRDGVVRHMSDIYYKGAECE
                     NF"
                     /name="virB11"
     gene            1..1863
                     /gene="taxB"
                     /locus_tag="PSR34_RS24675"
                     /old_locus_tag="PSR34_24675"
     CDS             1..1863
                     /gene="taxB"
                     /locus_tag="PSR34_RS24675"
                     /old_locus_tag="PSR34_24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxB"
                     /protein_id="WP_170324155.1"
                     /translation="MKTFNKSQSVFIFAVMLVAAWFAGSFIFFVLYFSQIKNLKPLKAV
                     FRSIDAYRMDIFTDSLTLSVVTTDIRVLAFVALGAGFVVSLIIPVAALIKLNEKKENIF
                     GDARFATINDIRESNSFTLDGDEKDGIIVGIKDKKIIRYVGAAFSAMGAGTRAGKGAGI
                     VITNLMKYWWSVIILDPKRECFNITSLIRKVILGHEVYKFDPFSSVTHRFNPLYYVSMG
                     TSEGFNQLENLSLIIYPYKTDGADAGSYLNKTAGGVFKSYAVALWFMIKNDKAGLKTLD
                     IEPVFSMSKINQLFERAEPEHLLSFMNDIRSELRGKDKTLADIGVAGLKAFIEMEDKTK
                     AQLKSNFLNGLSPFANPNVANATDGNDFDLRQVRKKRMTIYFCISGDNARLAEKITNIF
                     FQLAIQVNLEKMPTDDPEIKHDCLFLLDEFPSIGAVDYIKSKSGLIAGYKLKLLIVYQV
                     GSQLEEIYSYAGSKTLLASAPCKIVYSASDVKDARELSEAMGTRTVTIGSKSKSRSRGG
                     TSRSESESLIERPLVTTNELLTLKFSEEILAMKGENPIRCEKAFYYLNEYFFGDFVKVA
                     PELANIFPRKKGKLVMPPQKVFENEIVAKGYLVVKDVPDLDKAA"
                     /name="taxB"
     gene            1872..2294
                     /locus_tag="PSR34_RS24680"
                     /old_locus_tag="PSR34_24680"
     CDS             1872..2294
                     /locus_tag="PSR34_RS24680"
                     /old_locus_tag="PSR34_24680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="WP_015059903.1"
                     /translation="MMKKLVFLLISILAGCSSPPEPTPVQFEKANEVINPSLPYVPDFH
                     GVIKSDVSGKGWVYEITSLSGVQARTPTFYYALAHADRIVVTTHDAGLWFRIRDLLKME
                     GATAVVEWRNEKSFLPEQVKIVFIKAQNEVKNDWKK"
                     /name="cag pathogenicity island Cag12 family protein"
     gene            2278..2583
                     /locus_tag="PSR34_RS24685"
                     /old_locus_tag="PSR34_24685"
     CDS             2278..2583
                     /locus_tag="PSR34_RS24685"
                     /old_locus_tag="PSR34_24685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_001350475.1"
                     /translation="MTGKNRVVLPALLALGLISTNANASDPCASVLCLYGKAVGQGGGS
                     ECRSAEKDFFNILKKKKGSIRWSKTFDARKAFLNQCSTADPAAISKIMSKFGRVRG"
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            <2696..2890
                     /locus_tag="PSR34_RS24690"
                     /old_locus_tag="PSR34_24690"
                     /pseudo=""
     CDS             <2696..2890
                     /locus_tag="PSR34_RS24690"
                     /old_locus_tag="PSR34_24690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /name="ATP-binding protein"
     gene            2894..3466
                     /locus_tag="PSR34_RS24695"
                     /old_locus_tag="PSR34_24695"
     CDS             2894..3466
                     /locus_tag="PSR34_RS24695"
                     /old_locus_tag="PSR34_24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001198960.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKENPVKELPDIDESAHPFH
                     ICYGGYLLNPGVSDSDISRKIKDQAGFWLAAIDDTRMDCHSIAYYDIHTLPLISCGHQK
                     IVPFAALIKADECIISKIASYSGFAVTAFLRIKEWDIATNILNREGIFAFNGCERRFRV
                     VSKDNWQHTVSEERAIRCAKRLIQCKG"
                     /name="hypothetical protein"
     gene            3471..5624
                     /locus_tag="PSR34_RS24700"
                     /old_locus_tag="PSR34_24700"
     CDS             3471..5624
                     /locus_tag="PSR34_RS24700"
                     /old_locus_tag="PSR34_24700"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="WP_001235745.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDNAIVTNCFGHII
                     ESQPPENYNPEYKAWKVETLPLRLYPVKYQPVESAAKQVKTILELIRRGDVTEIVHAGD
                     PDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANLKDNRDFKGLYLKALARS
                     VADAVYGFSMTRAYTIPAKARGYQGVLSVGRVQTPVLGLIVNRTRANQNHKSSFYYTMT
                     GVFQRGADVIRANWKPGEFAPLTDRKLLDKAWADGTAASLAGKPATVEAAATDDKKTAA
                     PLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQFSEAPQV
                     IDALKSVFPQSLDIDSARKSKAFNSAKVTAHTAIIPTASVPDVNALSTDERNVYLAIAQ
                     HYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGAETTDEGESEDNDDSA
                     FELLCKIRTGETLTTKEVIVNEKKTTPPPLFTEASLLAALVRVADFVTDPTIKKLLKDK
                     DKDKKDEHGGIGTPATRAAILETLKKRNYITLEKGKLIPTDTGYALIDALPGIAVNPDM
                     TALWSEKQAAIENGDLTVEQFINDLYGELTGMISDVDLGEMKIEPAAPAGQFQRLDSPC
                     PSCGKHIVIRPKGYFCTGCEFKIWSEFSGKKITQAQAEKLVKSGKTDLIKGFKKKSGGT
                     YDTVLVLEDKKTGKLGFPARAKK"
                     /name="type IA DNA topoisomerase"
     gene            5621..5827
                     /gene="hha"
                     /locus_tag="PSR34_RS24705"
                     /old_locus_tag="PSR34_24705"
     CDS             5621..5827
                     /gene="hha"
                     /locus_tag="PSR34_RS24705"
                     /old_locus_tag="PSR34_24705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="WP_000850859.1"
                     /translation="MKTKQEWLFQLRKCTSRDTLEKVIEINRYKLPLSESEAFYSAADH
                     RRAELVMNKLYDKVPSGVWKYVH"
                     /name="hha"
     gene            5845..6309
                     /locus_tag="PSR34_RS24710"
                     /old_locus_tag="PSR34_24710"
     CDS             5845..6309
                     /locus_tag="PSR34_RS24710"
                     /old_locus_tag="PSR34_24710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293133.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0030527 - structural constituent of chromatin [Evidence
                     IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="WP_001293134.1"
                     /translation="MSELTKEDEYGIISRTMMNIRSLRVFAREIDFEQLLEMQEKLNVV
                     IEERREDAEREAAERQELEAKRQQAIEYIISLGLDPESLLAPVTADTVKTRRKAKGGVR
                     KAKYRFKDENGEIREWSGNGKRPLALQKLLDEGHFMEDFLIEKTKPEQAE"
                     /name="H-NS family nucleoid-associated regulatory protein"
     gene            6396..7100
                     /locus_tag="PSR34_RS24715"
                     /old_locus_tag="PSR34_24715"
     CDS             6396..7100
                     /locus_tag="PSR34_RS24715"
                     /old_locus_tag="PSR34_24715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            7883..7978
                     /gene="dqlB"
                     /locus_tag="PSR34_RS24720"
                     /old_locus_tag="PSR34_24720"
     CDS             7883..7978
                     /gene="dqlB"
                     /locus_tag="PSR34_RS24720"
                     /old_locus_tag="PSR34_24720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_021534057.1"
                     /translation="MINMLIIVLRAVVAVANALIAVLELIRQFID"
                     /name="dqlB"
     gene            8641..9288
                     /locus_tag="PSR34_RS24725"
                     /old_locus_tag="PSR34_24725"
     CDS             8641..9288
                     /locus_tag="PSR34_RS24725"
                     /old_locus_tag="PSR34_24725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000587671.1"
                     /translation="MIIVIGGDKGGTGKSHLATNLTVCLSQQGKRTGLVETDLNGSTKK
                     WNKRREQAGLPPVALNEAYGDISAKITKMADVAEILIIDTAGYDSTEFRTALKVADIVI
                     VPIDPLAQVEADSLQTVTKIVRDAQKINPRLAAHVLLYKCQPNTFSEQQELRDSLNSHD
                     YWLKPMKSTVSFLRAFVRAMNQGMGVHELKSNVSGASQAKAQIELLLKELEL"
                     /name="division plane positioning ATPase MipZ"
     gene            9363..9632
                     /locus_tag="PSR34_RS24730"
                     /old_locus_tag="PSR34_24730"
     CDS             9363..9632
                     /locus_tag="PSR34_RS24730"
                     /old_locus_tag="PSR34_24730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001086090.1"
                     /translation="MAKLELPELEQNQEVNATKFVQDAGKRPTENKTKLASFRIPNELL
                     EFIDNESKRLHRPKTKIIKAALLAYQDLDENELINLWFRADRND"
                     /name="hypothetical protein"
     gene            9793..10422
                     /locus_tag="PSR34_RS24735"
                     /old_locus_tag="PSR34_24735"
     CDS             9793..10422
                     /locus_tag="PSR34_RS24735"
                     /old_locus_tag="PSR34_24735"
                     /inference="COORDINATES: protein motif:HMM:NF022440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2806 domain-containing protein"
                     /protein_id="WP_223424520.1"
                     /translation="MSLIDLSLSGLSEPGTKLIEKISDAIGVLYEPTRIRKKAKAEAEA
                     KRTELISRLELEGIEKRAVERFLKRETKRQENIENITMQAAQSLSESDNVSDIDEDWIE
                     AFFRECEDISDEQMQMLWGRILSEEAKSKGSFSRRTLKLLSTISKEEANLITYFGKFVW
                     QANKLTPILFTDENGDTEGITFDKLSVLDSLGVIQQGIGYSLRYKL"
                     /name="DUF2806 domain-containing protein"
     gene            10596..12221
                     /locus_tag="PSR34_RS24740"
                     /old_locus_tag="PSR34_24740"
     CDS             10596..12221
                     /locus_tag="PSR34_RS24740"
                     /old_locus_tag="PSR34_24740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_049589868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase MCR-1.1"
                     /protein_id="WP_049589868.1"
                     /translation="MMQHTSVWYRRSVSPFVLVASVAVFLTATANLTFFDKISQTYPIA
                     DNLGFVLTIAVVLFGAMLLITTLLSSYRYVLKPVLILLLIMGAVTSYFTDTYGTVYDTT
                     MLQNALQTDQAETKDLLNAAFIMRIIGLGVLPSLLVAFVKVDYPTWGKGLMRRLGLIVA
                     SLALILLPVVAFSSHYASFFRVHKPLRSYVNPIMPIYSVGKLASIEYKKASAPKDTIYH
                     AKDAVQATKPDMRKPRLVVFVVGETARADHVSFNGYERDTFPQLAKIDGVTNFSNVTSC
                     GTSTAYSVPCMFSYLGADEYDVDTAKYQENVLDTLDRLGVSILWRDNNSDSKGVMDKLP
                     KAQFADYKSATNNAICNTNPYNECRDVGMLVGLDDFVAANNGKDMLIMLHQMGNHGPAY
                     FKRYDEKFAKFTPVCEGNELAKCEHQSLINAYDNALLATDDFIAQSIQWLQTHSNAYDV
                     SMLYVSDHGESLGENGVYLHGMPNAFAPKEQRSVPAFFWTDKQTGITPMATDTVLTHDA
                     ITPTLLKLFDVTADKVKDRTAFIR"
                     /name="phosphoethanolamine--lipid A transferase MCR-1.1"
     gene            12293..13015
                     /locus_tag="PSR34_RS24745"
                     /old_locus_tag="PSR34_24745"
     CDS             12293..13015
                     /locus_tag="PSR34_RS24745"
                     /old_locus_tag="PSR34_24745"
                     /inference="COORDINATES: protein motif:HMM:NF028536.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family protein"
                     /protein_id="WP_159197015.1"
                     /translation="MPYLSNKRLLAEMSIALVMAIVATLTLEHSQINLMVADWFYLGMG
                     HWMVAKQAFLPDLLLYSGLKKLLMAMLIYLLVATICRAYHEKKGNAITAKWLVPVTKFR
                     VRELAYLVLTLILVPTVVASLKAYTHVVCPVHLTIFDGTLPYLPMLDSMRNTIPDKCFP
                     AAHASSGFALFAFAFAPSLRRRRGAIIIVVMALGWAMGCYKMIIGDHFLSHTVVSMMLA
                     WAMSAGLAWVFFKKGEQV"
                     /name="PAP2 family protein"
     gene            complement(13406..13933)
                     /locus_tag="PSR34_RS24750"
                     /old_locus_tag="PSR34_24750"
     CDS             complement(13406..13933)
                     /locus_tag="PSR34_RS24750"
                     /old_locus_tag="PSR34_24750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="WP_000577043.1"
                     /translation="MIFNRNKKRDEQHNSAVVLPSAENVFSDFIKNNPALTTNAFTAIG
                     EVDCFTKVPLLSNNESEFLGILSRNIIPDYSVFPKIRLIEFVRPHGSEEAVKDLIREVQ
                     NITCDFVLKSYDETVLAVIQFGETATVRQQKKKLIIQRVCAMTGISFFEFKNTVDMMGS
                     EDFCQFLRDDEE"
                     /name="DUF2726 domain-containing protein"
     gene            complement(14068..14472)
                     /locus_tag="PSR34_RS24755"
                     /old_locus_tag="PSR34_24755"
     CDS             complement(14068..14472)
                     /locus_tag="PSR34_RS24755"
                     /old_locus_tag="PSR34_24755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012421203.1"
                     /translation="MKDNIVDFQRIRDSMNDHSTDVANDAVYSDKKKISRFFYLLTPVR
                     YLLAASLYLVVVIPLGILAGFRFLLQFLGGIAVMGLTIAWYMGADVSSYVVLAAWIALT
                     VCGCAEQIFEWWIESRFAFRLFGIGRTPEE"
                     /name="hypothetical protein"
     gene            complement(14485..15351)
                     /locus_tag="PSR34_RS24760"
                     /old_locus_tag="PSR34_24760"
     CDS             complement(14485..15351)
                     /locus_tag="PSR34_RS24760"
                     /old_locus_tag="PSR34_24760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473366.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001353743.1"
                     /translation="MTTNKTSLSRLTKVRHRNELNSTLSTLPMAAKKVLFLAMCQINSK
                     NEFDDDHIFYVTVADYIKWVQVKPDAAYLALRDGSNILDTTLLKLKHDEILELSSDLGF
                     KFTKSNVPDSMNLSLTVFSTYYRNEGRVGIKFTKEAKRFLCKLIGEEKRYTTQVLLSVV
                     RLTSVNAASLYQLIRKIYSNNSRANSFEMTIDELKDELNLYTIGAGGVKDYKYPDYPAF
                     KRDVLNKSVKEIMKHTEVKNLSFVVSEKIGRKVYKLKFSYTIGYEGDTREDSEFTNMFD
                     KMYPPEN"
                     /name="replication initiation protein"
     gene            16766..17281
                     /locus_tag="PSR34_RS24765"
                     /old_locus_tag="PSR34_24765"
     CDS             16766..17281
                     /locus_tag="PSR34_RS24765"
                     /old_locus_tag="PSR34_24765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="WP_001025395.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELMK
                     AVNAAFDVLMANIDKINQFQSTDEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVWIS
                     GETITHKETLKEIGCKWAAKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRRATG
                     WQRVETRA"
                     /name="J domain-containing protein"
     gene            17386..17928
                     /locus_tag="PSR34_RS24770"
                     /old_locus_tag="PSR34_24770"
     CDS             17386..17928
                     /locus_tag="PSR34_RS24770"
                     /old_locus_tag="PSR34_24770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001083906.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCSSRIKLTSPQFKNY
                     SVHIRMEKDRFSVVGSVDSRSWRSPHHVCTLSRKRNPVDIAADIERKILVNASQEVLQA
                     IEYEKHQVEKKDEILILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGDSYDLQIR
                     GLSIDQLVKITGYLKQL"
                     /name="hypothetical protein"
     gene            17925..18176
                     /locus_tag="PSR34_RS24775"
                     /old_locus_tag="PSR34_24775"
     CDS             17925..18176
                     /locus_tag="PSR34_RS24775"
                     /old_locus_tag="PSR34_24775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001230707.1"
                     /translation="MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDA
                     VLNENELQHIDFTCAGLREDDETPVKNLIKEFMLNETR"
                     /name="hypothetical protein"
     gene            18163..18390
                     /locus_tag="PSR34_RS24780"
                     /old_locus_tag="PSR34_24780"
     CDS             18163..18390
                     /locus_tag="PSR34_RS24780"
                     /old_locus_tag="PSR34_24780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000801440.1"
                     /translation="MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQN
                     SQSLSLVEFEFLLLLAGEHPEYVLCKRESK"
                     /name="hypothetical protein"
     gene            18844..19143
                     /locus_tag="PSR34_RS24785"
                     /old_locus_tag="PSR34_24785"
     CDS             18844..19143
                     /locus_tag="PSR34_RS24785"
                     /old_locus_tag="PSR34_24785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000650309.1"
                     /translation="MKCSSVFTSTTNHVFTFERVTLCTIILMHKDTGQQYVVIFTDNNK
                     IRDYKAGIVPQFGELKQSDVDLVLFYRDEYEKYFDSLKDGDECLSFKDFIECLC"
                     /name="hypothetical protein"
     gene            19234..19584
                     /locus_tag="PSR34_RS24790"
                     /old_locus_tag="PSR34_24790"
     CDS             19234..19584
                     /locus_tag="PSR34_RS24790"
                     /old_locus_tag="PSR34_24790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000854259.1"
                     /translation="MKTLKQAAMQFASDLRNNRCFKAAYNDALLELDREDLNAIVQCTF
                     LFEPKRTLAELDKLIKKIEVEEDYEFCVSLDKGKGLHEVRTFKSEREALDAYRILTLYG
                     YTVTIEKKEGND"
                     /name="hypothetical protein"
     gene            19889..20138
                     /locus_tag="PSR34_RS24795"
                     /old_locus_tag="PSR34_24795"
                     /pseudo=""
     CDS             19889..20138
                     /locus_tag="PSR34_RS24795"
                     /old_locus_tag="PSR34_24795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653200.1"
                     /GO_function="GO:0003729 - mRNA binding [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family toxin"
                     /name="type II toxin-antitoxin system HicA family toxin"
     gene            20135..20482
                     /locus_tag="PSR34_RS24800"
                     /old_locus_tag="PSR34_24800"
     CDS             20135..20482
                     /locus_tag="PSR34_RS24800"
                     /old_locus_tag="PSR34_24800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="WP_000681613.1"
                     /translation="MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQ
                     EFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPELHRRVAEMAMEEDLSLNAFVNKAL
                     EKEVSNHRAGA"
                     /name="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            20843..21346
                     /gene="taxA"
                     /locus_tag="PSR34_RS24805"
                     /old_locus_tag="PSR34_24805"
     CDS             20843..21346
                     /gene="taxA"
                     /locus_tag="PSR34_RS24805"
                     /old_locus_tag="PSR34_24805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxA"
                     /protein_id="WP_000781813.1"
                     /translation="MKMIAFRLSDDELKFAEHNAILSGFTSINAFAKHNVLNIETKPVN
                     IPVNNEPAKLVSVRLYPHEIELVKRNAALHGMSMSREIAIRVRQSLLKSEVCLYPDEVK
                     ELKKLSTAVDRVGRNIHFIIKGERFCTVNDPDFRKDVIEVIELCKQIDSKLETLTKSVV
                     NRFG"
                     /name="taxA"
     gene            21350..22579
                     /gene="taxC"
                     /locus_tag="PSR34_RS24810"
                     /old_locus_tag="PSR34_24810"
     CDS             21350..22579
                     /gene="taxC"
                     /locus_tag="PSR34_RS24810"
                     /old_locus_tag="PSR34_24810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase/nickase TaxC"
                     /protein_id="WP_001329504.1"
                     /translation="MGVYVEQEYRIKRAKGTGRDPKSPKLSGRHIHASKSAFNHKVRHG
                     ADKKAYTWTPTKEVTFKITGSGKTAAGIKNGIDYITRNGELEAYCYDGKGSEQTGKGED
                     FNREFTSTLSKGNDYSRTYRGENIDHVKNMVFSPPPEAGVSREDALKATVEFLKETYPN
                     HAFVAVYHDDKEDHPHVHVNIKLRDEETGRRLRLTKSETRKFRNGFHRKLKGMGYDVTA
                     TWKKDPERKREIERLQAENPKRLRNVYKVVDFGETSYQNKAGEKRTPFLTYETLKGGKQ
                     VTIWGKDLKNHFESEKLQPGTLIKVKKLAPTLVRSPMFNDDGTVAGYRETHRNNWQIEN
                     IALERNRERQVHERETRQPEESDVKKQLSRKHEQGHNIGFALEHGFTKDSEEHKKLRIQ
                     QERNWKGLGF"
                     /name="taxC"
     gene            22656..22949
                     /locus_tag="PSR34_RS24815"
                     /old_locus_tag="PSR34_24815"
     CDS             22656..22949
                     /locus_tag="PSR34_RS24815"
                     /old_locus_tag="PSR34_24815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134733.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]; GO:0016987 - sigma factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="WP_060598542.1"
                     /translation="MTEHDAICISGLHQIFSDEEHLSEQQKDIILMYAYGYTLNEIADF
                     KGLKPSTVRKYLDSVRAELGGVSLAGIRTLVLIRTNALLVSSLSRISERGNL"
                     /name="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            22946..23161
                     /locus_tag="PSR34_RS24820"
                     /old_locus_tag="PSR34_24820"
     CDS             22946..23161
                     /locus_tag="PSR34_RS24820"
                     /old_locus_tag="PSR34_24820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000760375.1"
                     /translation="MKKYLLFALPFFVVGCSEEVKSVDWWGQHLTEAKQKQAECEKSGS
                     DSQNCKNVKQALFIQSQKDAPVPTFD"
                     /name="EexN family lipoprotein"
     gene            23517..24137
                     /locus_tag="PSR34_RS24825"
                     /old_locus_tag="PSR34_24825"
     CDS             23517..24137
                     /locus_tag="PSR34_RS24825"
                     /old_locus_tag="PSR34_24825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_000157549.1"
                     /translation="MASLFPALLLCAASVHPDTINDIARVESGFNPYAVAEIIPERERG
                     SYGKSVISHMPKSKEEALTVIREIENRKRRYSVGLMQITSSNFSFYSTSAEKLLDSCEN
                     LSVFEKIIVDCYKRGRSLENALSCYYTGNFSNGKRKEKEFNNTSYVERIGYTGNEKKYV
                     VPGTRSNGGEQRKNRSHNASVIWPETILKSAFVDNSHPTKVIN"
                     /name="lytic transglycosylase domain-containing protein"
     gene            24151..24477
                     /locus_tag="PSR34_RS24830"
                     /old_locus_tag="PSR34_24830"
     CDS             24151..24477
                     /locus_tag="PSR34_RS24830"
                     /old_locus_tag="PSR34_24830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_000008647.1"
                     /translation="MSFKKSVCNRISSSVTAVMQRKNKVVMAVAGATVASPAFADGFSK
                     AETLLEKVKTGLSGLSLVTVTIACLWVGYKVLFGGSTIRECSPIIIGAIVIASAAEVAS
                     MMVN"
                     /name="TrbC/VirB2 family protein"
     gene            24496..27243
                     /locus_tag="PSR34_RS24835"
                     /old_locus_tag="PSR34_24835"
     CDS             24496..27243
                     /locus_tag="PSR34_RS24835"
                     /old_locus_tag="PSR34_24835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="WP_000105985.1"
                     /translation="MSTLYKAMTRPAMYVGVPVVPLTVVAGALFLAGVYISKLIWLAIP
                     VAVFVLRMITKQDDHIFNLYFLKLKMLGNSVCNRFFGARAFLSGQYEAVEIDEFVNAMK
                     LNERITTGKYIPYSSHVDKNIVKTKNGDYVATWQLMGINFESISAEMLETIDSQVATLV
                     RSFSGLPVSFYNHSCRASFYDAFTTKSGNKYADIISDCYYGSMKKNKFKGNTLYFTLIY
                     RPDGRVEKLEKRKKSIKEKKDDINIHVKRMNEMINTFSGALDKFTCKLLGMYEENGKVF
                     SSQLSFYNYLLTGKLQKIRVTDSPVYNVLGGVDVFFNHDTGQICRIDGNKFFRSIEIKD
                     FCSETASGVFDVLQYSDADYIITHSYTSMSKSEALSTIKRAEKQLKSTEDDAVTQLQEL
                     EKAKNDIVSGDISFGYYHFTLMVMADSIRELDESVSKITADFTDLGIIPALSTMSLPAA
                     YFAQLPAVFHLRPRLSPVSNVNFVELASFHNFYQGKRDKNCWTEAVAILKTPSKQAYYL
                     NLHNSVLFKDETGEKNLANTKVIGTAGSGKTMFLSYLACSLQKYNNPETFADSAKNKKL
                     TCVFLDKDRGAELCIRMLGGEYYTVKSGEPTGWNPFALEATKRNRIFVKQLMEILCTRN
                     GERLSTRERLLISESVDAVMDFPPGEMREYGITRMLEHLMQRDDRDEQENGIILRLSQW
                     ANGQAHGWVFDNAKDTFNIQHVNNFGIDGTEFLDDPMVCAPITFYLLYRITQLLDGRRL
                     VIFLDEFWKWLQDEAFSDFVYNKLKTIRKLNGLVIPATQSPDEILKNKISRAVVEVCST
                     SIYLANPDADYNDYVEGLKLTPEEFNIVKNLDPMSRQFLIKKSSLKKGDGKSFSALATL
                     DLSGLGGYLKILSASADNLEIFESIYHEGMEPDDWVPEYLERAI"
                     /name="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
     gene            27255..27971
                     /locus_tag="PSR34_RS24840"
                     /old_locus_tag="PSR34_24840"
     CDS             27255..27971
                     /locus_tag="PSR34_RS24840"
                     /old_locus_tag="PSR34_24840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_274040078.1"
                     /translation="MKRVKTLMLISLLTVSFYSSAGIPVAIDASPEWAVEAARWTERLK
                     QWSETAQHYQSQIQAYKDQLATATGIRNIAAFTNELSNLQSELTNIYKQGNSYISDFTS
                     NPEGALSSQAKNLFSKYGAFDMCNTGYERNDNLCKARIVSTAASIEQGNEINKQLSSAM
                     SQIQSLSSRIEASKDIKESQDLANALQAQSLKMQAIKMQYDVWNNKNKADHEMLVTQEQ
                     EAFIKQQKEAEPLTFD"
                     /name="type IV secretion system protein"
     gene            27983..29077
                     /locus_tag="PSR34_RS24845"
                     /old_locus_tag="PSR34_24845"
     CDS             27983..29077
                     /locus_tag="PSR34_RS24845"
                     /old_locus_tag="PSR34_24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677777.1"
                     /GO_process="GO:0030255 - protein secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_000046889.1"
                     /translation="MAQGFFVKYNSTVMDSVDKISSSYQTQFANDIMSLATVSVTLYVL
                     WKGYQILASKTQTPLQDLVWDLSKFAIIIMFITNADGYLTAATDALQGMKDGFSGGVSV
                     WQTLDNLWKSTQNLGAEIYSLDKSTYVKDQGVVGQFLIWTGSLILMAVSVVVFLTADVT
                     MKLLIITAPIFIFCLMFGFIRSMFNNWLQSLFSSILTVLFASLVIRIAMDFQGMILSHA
                     IRAAQTGNVNLVSTGAMGFMAGFLGALLVLIAKGFAVQLAGAGVEGAVQGAAMMGLGAA
                     GMATGKSLMLGGRAGLGFGLGMAGRTGMNSLSGKAGNLMGRGARTAAEWAGEKGYQALA
                     PTGALGMKARRLASLEKARARNAA"
                     /name="type IV secretion system protein"
     gene            29592..30278
                     /locus_tag="PSR34_RS24850"
                     /old_locus_tag="PSR34_24850"
     CDS             29592..30278
                     /locus_tag="PSR34_RS24850"
                     /old_locus_tag="PSR34_24850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_001290765.1"
                     /translation="MSEKIEKKIETAKSFERQLYAENERSKKNAWRVAVAASLLSLCLG
                     AAICVLVPLKEKELAIVAVGEKTGRTELITQVKQEKILQSEALGRYFVNTYITLREGYN
                     YPSLQYDYETVQLYSSNTVKDDYLRLYNSDMAPDKIYHNNGSYVAVEVISNIISDATAP
                     DKLASVRFKKTTRNFTTGQVSVSYWTARVTYRFEPEKSVKSSSRELNPLGFTVTSYQTD
                     REVRGE"
                     /name="type IV secretion system protein"
     gene            30275..31183
                     /gene="virB9"
                     /locus_tag="PSR34_RS24855"
                     /old_locus_tag="PSR34_24855"
     CDS             30275..31183
                     /gene="virB9"
                     /locus_tag="PSR34_RS24855"
                     /old_locus_tag="PSR34_24855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="WP_001329500.1"
                     /translation="MMKKLFIATCLLLPGLVYSAATPLPSGFDARMQTVSYNGANTTVI
                     RSKTGFLTSVVFDEGEAVISAKAGFPAGWEITTDDNVVYINPRPVVQEQEGDEGEKLKK
                     VFQPTEKEWDTNLFVRTTKRIYSLDLILLSEEKQAQPAYVVQFRYPSEIAKKNAEEVRL
                     AKEKQEKLRQKKLISESFEKADAPKNWNYFMRVNEKYDSRRIAPDFAYDNGIFTFLGFN
                     SGKVFPAPFAVRDGQEQTLAFNVETKGKYKIMVIHNVNDKFVLRYGNSVVGVVNKSFGK
                     VLTDQRNTSSPAVERVEVNND"
                     /name="virB9"
     gene            31176..32294
                     /gene="virB10"
                     /locus_tag="PSR34_RS24860"
                     /old_locus_tag="PSR34_24860"
     CDS             31176..32294
                     /gene="virB10"
                     /locus_tag="PSR34_RS24860"
                     /old_locus_tag="PSR34_24860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="WP_250308750.1"
                     /translation="MTDMKKTDDLVDDANQSKLESKPVHDIGDIRNKNNRMRSGFLFGL
                     MAVLVIGIFALKTYKNYFADDQQKASESTGDTSISQVSKIRTGLGQNFDPVENKAIINP
                     GATGSGNTVSDGHKEVPQEGFRKYLSIPVAGQGGSQTGASGCSRTSQTSEPQEERKESK
                     ENVPGKSGMKVTAINLDPDLYIEENRLIPCALTTRFVSDVAGRISCVFTEDVWSANHHT
                     KLLEQGTKAFGRYQTGTLNHGQGRMFVMWEQLRTPDNKRIDMVNTAAAGPLGEAGIDGW
                     IDSHFWERFGGSLMLSMVQDVAAAAADNAPGKDRNVDYTENSRQAMAEMAKVALENSIN
                     IPPTMYKNQGDIITIMVGEDIDFSDIYELKVK"
                     /name="virB10"
     ARG             10597..12218
                     /name="mcr-1.1"
                     /gene="mcr-1.1"
                     /gene_name="phosphoethanolamine--lipid A transferase
                     MCR-1.1"
                     /tool="amrfinderplus"
                     /drug_class="COLISTIN"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(14486..15357)
                     /name="IncX4"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          21351..22576
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.511"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..1860
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.71"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          27984..29050
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.719"
                     /coverage="99.0"
                     /evalue="2.69e-172"
     Typing          29593..30275
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.4399999999999998e-137"
     Typing          30276..31180
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.669"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgaaaactt ttaataagtc gcaatctgtt tttatattcg ctgttatgct tgttgcggca
       61 tggtttgcag gttcatttat tttctttgtg ctttacttca gccagataaa gaatctgaaa
      121 ccattgaagg ctgtgttcag atccattgat gcgtacagga tggatatttt tacagacagc
      181 cttactttat ctgttgtgac tacggatatc agagttttag cctttgttgc acttggagca
      241 ggatttgtcg tgtctcttat catccctgtt gcggcgttga taaaactgaa cgagaagaaa
      301 gaaaatattt tcggtgatgc gcgatttgca acaattaatg atattcgtga gtctaacagt
      361 ttcacgcttg acggtgacga aaaagacggg attatcgttg gtataaaaga caagaaaatc
      421 atacgctatg tgggagcagc attcagtgct atgggagccg gaacaagggc tggtaaaggt
      481 gctggtatag tcatcactaa tcttatgaag tactggtgga gtgttattat tctcgatcca
      541 aaaagggagt gttttaatat caccagtctt atcaggaagg tgattttagg tcatgaggtt
      601 tataaatttg atccctttag ttcggtaacg caccgcttta accctttgta ttatgtcagc
      661 atgggaacga gtgaaggttt taatcagctt gaaaatcttt cgttgattat ttacccctat
      721 aagacagatg gtgcggatgc tggtagctat cttaataaga cggcgggtgg tgtttttaaa
      781 tcttatgctg tcgctttatg gtttatgatt aagaatgata aggctggact aaaaacgctt
      841 gatattgagc ctgtcttctc aatgtctaaa attaatcagc tttttgagcg tgcagaaccc
      901 gaacatcttc tttcattcat gaatgatatt cgttcagaat taagggggaa agacaaaacg
      961 cttgctgata ttggtgtcgc tggccttaag gcattcattg agatggagga taaaacaaaa
     1021 gcacaactca aaagcaactt tcttaatgga ttatcaccgt ttgctaaccc caatgtggca
     1081 aatgctactg atggaaatga ttttgatttg cggcaggtgc ggaaaaagcg gatgacaatt
     1141 tatttctgta tttctggtga taatgccagg ttggctgaaa aaataacaaa tattttcttt
     1201 cagcttgcta tacaggtgaa tcttgaaaag atgccaacgg atgatccgga aataaagcat
     1261 gactgccttt ttctactgga tgaattcccg tcaattggtg ctgtagatta catcaagagt
     1321 aaatctggtc tcattgctgg ttacaagctg aaattgttga ttgtttacca ggtcggctct
     1381 cagcttgagg aaatctacag ttatgcaggc agtaaaacat tactggcaag tgcaccctgc
     1441 aaaattgttt attccgcttc tgatgttaaa gatgcgcggg agttatcaga agcgatgggg
     1501 acacgaacag taactatcgg ttcgaaaagc aaaagtcgta gtcgtggagg aacatcgaga
     1561 tctgaatcag aaagccttat tgagcgtcca ctggtaacaa caaatgaact acttacactg
     1621 aaatttagtg aagaaatcct ggcaatgaag ggagaaaacc cgattcgttg tgaaaaggca
     1681 ttctactatc tcaatgagta tttctttggt gactttgtga aggttgctcc ggaacttgca
     1741 aacattttcc ccaggaagaa aggaaagctg gttatgcctc cacagaaagt atttgaaaat
     1801 gaaatcgtcg caaaagggta tctggtagta aaagatgttc ctgatctcga taaagcggca
     1861 taaaggagaa catgatgaag aagttagtgt ttttattgat ttccattctt gctggttgtt
     1921 catcaccgcc agagccaaca cccgtgcagt ttgaaaaggc aaatgaagtg atcaatccct
     1981 cattgccata tgtccccgat tttcacggtg tcataaagtc agacgttagc ggtaaaggat
     2041 gggtttatga gatcacttcc ttatccggag tacaggccag aacgccgacg ttttattacg
     2101 ctctggctca tgcggacagg attgtagtga caacacatga tgctgggctg tggtttcgaa
     2161 tcagagatct gttgaagatg gaaggggcaa cagcagtcgt ggaatggcga aacgagaaaa
     2221 gttttttacc tgaacaggta aaaatagttt tcattaaagc acaaaatgag gtgaaaaatg
     2281 actggaaaaa atagagtggt tttaccagcc ttgctggcgt taggattaat atcaacaaat
     2341 gctaatgcat ctgacccttg cgcttcggta ctttgcttat atggaaaagc agtcgggcaa
     2401 ggtggaggga gtgaatgcag aagtgctgaa aaggattttt ttaatattct gaaaaagaaa
     2461 aaaggaagca ttcgctggag taaaactttt gatgcccgta aagcatttct gaatcagtgt
     2521 tcaacggctg atcctgctgc tatatcaaaa atcatgagta agtttggtcg tgtcagaggt
     2581 taattaaaac ctgataaccg cgccaacggg tatcagggta ctgcaagtat cacatttcgc
     2641 gccaacgaaa tgtgatgatt agttacaatc actgaggtta gaaatgaaag taaccaaatc
     2701 tattatagct attttattgt gcttatctgc tggtagtgca taagtggatg taataaaaaa
     2761 tgctcgtgcc tttttgaaag gaaccctcga tctgagcgtt caggaagcag atgaagatat
     2821 agaggaaagg gaagaacttt ataaaaagaa cggggcacaa cctgactatt taagttacct
     2881 ggaggattaa cacatgcaat atgcattgtt tgatggattt gaacgaaagt ttttgctgga
     2941 tgctcttgaa ttcggtgttc tgaaggactg gaaagaaaat ccggtaaaag aacttcctga
     3001 tattgatgaa tctgctcacc ccttccatat ctgttatggt ggatatttat taaaccctgg
     3061 tgtttcagat tcagatatta gcagaaaaat aaaagaccag gcaggattct ggctggcagc
     3121 tattgatgat acccgcatgg attgtcattc aatagcttat tatgatattc acacactccc
     3181 tctgatttcg tgtggtcatc aaaagatagt tccttttgcc gcgttaataa aagctgatga
     3241 atgcatcatt tcaaaaattg cttcgtattc tggttttgct gtaacagcct ttttgagaat
     3301 taaagagtgg gatattgcaa ctaatatact taaccgtgag ggaatttttg cctttaatgg
     3361 ctgtgagcgc agattcagag tagtaagtaa agataactgg caacatacag tatcggaaga
     3421 acgcgctatc cgttgtgcca aaagattaat tcaatgtaaa ggataacaaa atgagacttt
     3481 ttatcgcaga aaaacccgca gtagcaaatg atattgttaa ggcacttggt ggcaatttta
     3541 cccgccatga tggctggttt gaaagtgata acgccattgt gactaactgt tttggtcata
     3601 ttatcgaatc acaaccgccg gaaaactata atcctgaata caaagcctgg aaggttgaaa
     3661 cgcttccttt acgtctttat cccgtgaagt atcagcctgt tgaaagtgca gcaaaacagg
     3721 ttaaaacgat tctcgaactt atcagacgtg gagacgtgac tgaaattgtt cacgctggcg
     3781 atcctgatga tgagggacag ctactggttg atgaagtcct ggaatatgca ggcaacacaa
     3841 aacccgtaaa gcgcgttctg attaacgaca acacgcttcc ggcagtgaaa aaggcactgg
     3901 caaatcttaa agacaatcgt gatttcaaag gactttacct taaggcgttg gcgcggtcag
     3961 ttgccgatgc cgtctatggc ttctctatga cacgtgcgta caccattccg gcaaaagcca
     4021 gaggatatca gggcgttctg tctgtcgggc gcgtccagac tcccgttctt ggcctgattg
     4081 tgaatcgtac ccgtgctaac cagaaccata aatccagttt ttactacacc atgaccggag
     4141 tctttcagcg tggtgctgat gttatcaggg cgaactggaa gccaggggaa tttgctccgc
     4201 tgacagaccg taaattactt gataaagcgt gggcagacgg aacggctgca tcccttgcag
     4261 gaaaaccggc tacagttgaa gcagcagcaa ctgatgataa aaaaacggct gcgccgttgc
     4321 cgtttaatct ggtcagactc cagcaataca tgaacaagaa gtttaaaatg acggcacaaa
     4381 aaacgctgga tattacgcaa caactacgcg aaaaatataa agcaattacg tataaccgct
     4441 ctgactgctc atatctttca gatgaacaat tcagcgaagc gccgcaggtt atcgatgccc
     4501 tgaaatcagt ctttcctcag tcgctggata ttgattctgc acgtaaaagc aaagcgttta
     4561 acagtgcaaa ggtgactgcg catactgcga taatcccgac cgccagtgtg cctgatgtta
     4621 acgcactcag caccgacgag cgcaatgttt acctggcgat cgcacaacac tatcttgttc
     4681 agttcatgcc tgaaaaagca taccaggaag tatcggttgc cattcagtgt ggtgatgagt
     4741 cgttctatgc ccgtgccaga aaaacaactg acagcggatt tgaggcgttt cttggcgcgg
     4801 aaaccacaga cgaaggtgaa tcagaagata atgatgattc cgcttttgaa ctgctctgta
     4861 aaattcgcac aggagaaaca ctgacgacaa aagaagttat tgttaatgag aagaaaacaa
     4921 caccgccgcc gttattcacc gaagcctcct tgcttgctgc gcttgttcgt gtcgcggatt
     4981 ttgtcactga tccaacaatt aaaaaattgt tgaaggataa ggataaagac aaaaaagatg
     5041 aacatggtgg cattggtacg ccagctaccc gcgcagccat tctggaaacg ctgaagaaaa
     5101 gaaactatat cacgctggaa aaagggaaac ttattcctac tgataccgga tatgcgctta
     5161 ttgatgccct gcctggtata gcggttaatc ctgatatgac agcattatgg tctgaaaagc
     5221 aggctgccat agaaaatggc gacctgacgg ttgaacagtt tattaatgat ctgtacggtg
     5281 aactgacagg catgatttct gatgttgacc tgggcgagat gaagattgaa cccgctgcgc
     5341 cagcagggca gtttcaacgc ctggactctc cctgcccttc ctgtggtaaa catattgtta
     5401 tcaggccgaa aggttatttc tgtaccggat gtgaatttaa aatctggagt gagttttctg
     5461 gtaagaaaat cacccaggca caggccgaaa aactggttaa atcagggaaa accgatttga
     5521 ttaagggatt taaaaagaaa agtggtggaa cgtatgatac agttcttgtc cttgaggata
     5581 agaaaacagg gaagctgggt tttccggcaa gggctaagaa gtgaaaacaa agcaggaatg
     5641 gctttttcag ttaagaaaat gtacatcaag agatactctt gaaaaagtta ttgagattaa
     5701 ccgttacaaa ctgcctttat cagaatcaga ggcattttat tctgccgcag atcatcgccg
     5761 agcagaactg gtgatgaata aactttatga taaggtgcct tccggcgtct ggaagtacgt
     5821 acactaaata agaggattaa ttatatgagc gaactgacta aagaagatga atacggcatt
     5881 atcagccgta ctatgatgaa tattcgttca ttgcgtgttt ttgcccgtga gattgatttt
     5941 gagcagttgc tcgaaatgca ggaaaagctc aacgttgtta ttgaagaacg tcgtgaagat
     6001 gctgaacgtg aagcggctga acgccaggaa cttgaagcca aacgtcagca ggccattgaa
     6061 tacatcatat ctttaggtct tgatcccgaa tcactccttg ctcctgtaac agcagataca
     6121 gtaaaaacca ggcggaaagc caaaggcgga gtaagaaagg ctaaataccg ctttaaggat
     6181 gaaaatggcg agatcagaga atggtctggt aatggcaagc gtccgcttgc tttacagaag
     6241 ctgcttgatg aggggcattt catggaagat ttcctcattg agaaaactaa gccggaacag
     6301 gcagaataac ccgccaagta tacatcacac agggcactgt tgcaaatagt cggtggtgat
     6361 aaacttatca tccccttttg ctgatggagc tgcacatgaa cccattcaaa ggccggcatt
     6421 ttcagcgtga catcattctg tgggccgtac gctggtactg caaatacggc atcagttacc
     6481 gtgagctgca ggagatgctg gctgaacgcg gagtgaatgt cgatcactcc acgatttacc
     6541 gctgggttca gcgttatgcg cctgaaatgg aaaaacggct gcgctggtac tggcgtaacc
     6601 cttccgatct ttgcccgtgg cacatggatg aaacctacgt gaaggtcaat ggccgctggg
     6661 cgtatctgta ccgggccgtc gacagccggg gccgcactgt cgatttttat ctctcctccc
     6721 gtcgtaacag caaagctgca taccggtttc tgggtaaaat cctcaacaac gtgaagaagt
     6781 ggcagatccc gcgattcatc aacacggata aagcgcccgc ctatggtcgc gcgcttgctc
     6841 tgctcaaacg cgaaggccgg tgcccgtctg acgttgaaca ccgacagatt aagtaccgga
     6901 acaacgtgat tgaatgcgat catggcaaac tgaaacggat aatcggcgcc acgctgggat
     6961 ttaaatccat gaagacggct tacgccacca tcaaaggtat tgaggtgatg cgtgcactac
     7021 gcaaaggcca ggcctcagca ttttattatg gtgatcccct gggcgaaatg cgcctggtaa
     7081 gcagagtttt tgaaatgtaa ggcctttgaa taagacaaaa ggctgcctca tcgctaactt
     7141 tgcaacagtg cctcacacag acatacacat aggtatgtct gtgtgacata cctatgtaca
     7201 gtgccagatg gcgttgctac gtgaaaaata acctacccta cccctgcttt tttcgcagat
     7261 tttacgtagc acaccgttgc tatagcattc atctaaaaag ctgaatgtcc ttttctgtcg
     7321 ctgttgcatt ttaattcact gaaaaatatt gagtaatgaa aatgccggta acgcgtcaag
     7381 ctgttggcca acgcgacagg cagtattgga agcaacaaaa aaaatgaaag aaaaatccct
     7441 caaatgaagg agaaaaaacg gaactacagc cgaacgacag gcacaaaaaa acccgactgg
     7501 taatcgggct tttttgcgtt ttccagtctt gagttggtgg ctctgaccgg agaaggttac
     7561 tctacaccta acgtttacat tttattcact atggtgctgt aaagcaacta aatgtggtta
     7621 aggaaccaca tttagtggtg taacgggtgt tttgtgtagc gtataatcag tcagttagtc
     7681 atgtagttgg cttgcaatcc tgctgttccc gtatctgcgg gtatgaacga ttttaaagtc
     7741 ctggctggat aaacaggtgg tgctgtattc ggtgctctgg caggaggagg tcactctaca
     7801 cctaacaatg cgagagagcc gttgtgacag gcagtgtatg agcaagcaac ggtgatttcc
     7861 tccagtttta ctggagaaac ggatgattaa tatgttaatc atcgttctca gagctgtggt
     7921 cgccgttgca aacgcgctga ttgcagttct ggaactgatc cgccaattca tcgattgatg
     7981 acaacggaaa cgtaactaag ggcaggaagt gaaccgcttc ctgcccttta acaaaaaagg
     8041 aggatttatg gattttatga gcattttggg gcgctgttta cttcttcctt tccctgttgc
     8101 gatgtttgtc ggttgcctgt ttcctggcat cgatgatcgc ttatccggaa tgcttatgag
     8161 tgttgctgtg ggatgtttat cgtggtatgt caccagaccg aagaaccgac agacaaaggc
     8221 cgcgtaagtg gcctttaatt gggataccct ttagttacaa cattctaata gctcattcca
     8281 tcctccgctc atccagcaaa cataacctcc ctgcccctcc ccactacaga aagtggcgcg
     8341 ccccgcccgt acgggcggaa tctaatcgct taattttgaa tgttgtaact aaacatactc
     8401 attgctgtat tgccacaagg tttcgcagca agggaggata ccgaaagtaa cgctgtcact
     8461 tttgctttca agtgaccaac caacgggtac atcacactga catacaataa tgtatttctt
     8521 catgatgtac atcattgtaa ttcattgtga catacactat agtatgtcca tgtgacatac
     8581 gcatgcgtac atcacataga aatacacatg agtaaatcac tgtgacatac aaggggtact
     8641 atgattatcg taatcggtgg tgataaaggc ggtacaggta aatcacatct ggcaaccaac
     8701 ttaactgttt gcctctcaca acagggaaag agaacggggc ttgttgaaac agacctgaat
     8761 ggctcgacca aaaaatggaa taaacgcaga gaacaggccg gattaccgcc cgtcgcatta
     8821 aatgaagcct atggcgacat cagtgccaaa ataacaaaaa tggcagatgt agcagaaatc
     8881 ctcattatag atactgccgg atatgacagc acggagttca gaactgcact aaaagttgca
     8941 gacattgtga ttgtcccgat cgacccgctt gcccaggtgg aagcagacag tcttcagact
     9001 gtgacaaaaa ttgtcaggga tgcgcaaaaa ataaatccca ggctggctgc acatgtgctc
     9061 ctgtataaat gccagccaaa tacgttcagt gaacaacaag aattaagaga ctctctcaac
     9121 agccatgatt actggcttaa accaatgaaa agcacggttt cttttctccg ggcctttgta
     9181 agagcaatga atcagggaat gggggttcac gagttaaaaa gtaatgttag cggtgccagc
     9241 caggcaaaag cacaaatcga actcttgctg aaagaactgg aactgtaaat catagtgaca
     9301 tacactaatg tacatcatca tgatatacat tagtgtgaaa caatcagatt agaaggtaca
     9361 tcatggcaaa gctggaacta ccagaactag aacaaaacca ggaagtgaac gccacaaaat
     9421 ttgttcagga cgcaggaaaa cgccccacag agaacaaaac aaaactggct tcattccgca
     9481 taccgaatga attgcttgaa ttcattgata acgaatcaaa aagactgcat cgtcccaaga
     9541 caaaaataat caaagcagca ttactggctt atcaggatct ggacgaaaac gaactcatta
     9601 atttgtggtt cagagctgac agaaatgatt aatcatagca agtggcccaa taaactcttt
     9661 atcattttct tttagatact tatctgaatg tactaacttc atcactttta aatgtagttg
     9721 tgattcattt ttagttactc ttgcaataaa atacccttgt acagttgttg cacgggtatt
     9781 taggagcact agatgagcct tatcgattta agtttatcag gattatcaga accaggaaca
     9841 aaacttattg aaaaaataag tgatgctatt ggtgtgcttt atgaacctac aagaatcagg
     9901 aaaaaagcga aggctgaagc tgaagctaaa cgcacagagc taatttctag gttagagctt
     9961 gagggaatag aaaaacgggc agttgagcgt tttctcaaac gtgaaacgaa acgacaggaa
    10021 aacattgaaa atataacaat gcaagcagca caaagtctat ctgagagtga taatgtttct
    10081 gatattgacg aagattggat agaggctttc ttcagggagt gtgaggatat tagtgacgaa
    10141 caaatgcaaa tgctttgggg aagaatttta tccgaagaag caaagtcaaa aggttcgttt
    10201 agtcgtagaa ccttaaaact attatccact ataagcaaag aagaagcgaa ccttattact
    10261 tattttggaa agtttgtttg gcaggcaaac aaactcactc caattttatt taccgatgag
    10321 aatggagata ctgaaggtat aactttcgat aaactttcgg ttttagactc cttaggcgtt
    10381 attcaacaag gcattggtta tagtttaaga tacaaattat aaatactctc aagtgtatat
    10441 tcagtatggg attgcgcaat gattgcctaa taaaatttct gaaatatttc tgtatcgcat
    10501 aattttttat atcagataaa ttgtactgga tttcttaaaa aattgcagta tgattgccgc
    10561 aattatccca ccgtttattt tttgagtagt ttctcatgat gcagcatact tctgtgtggt
    10621 accgacgctc ggtcagtccg tttgttcttg tggcgagtgt tgccgttttc ttgaccgcga
    10681 ccgccaatct tacctttttt gataaaatca gccaaaccta tcccatcgcg gacaatctcg
    10741 gctttgtgct gacgatcgct gtcgtgctct ttggcgcgat gctactgatc accacgctgt
    10801 tatcatcgta tcgctatgtg ctaaagcctg tgttgatttt gctattaatc atgggcgcgg
    10861 tgaccagtta ttttactgac acttatggca cggtctatga tacgaccatg ctccaaaatg
    10921 ccctacagac cgaccaagcc gagaccaagg atctattaaa cgcagcgttt atcatgcgta
    10981 tcattggttt gggtgtgcta ccaagtttgc ttgtggcttt tgttaaggtg gattatccga
    11041 cttggggcaa gggtttgatg cgccgattgg gcttgatcgt ggcaagtctt gcgctgattt
    11101 tactgcctgt ggtggcgttc agcagtcatt atgccagttt ctttcgcgtg cataagccgc
    11161 tgcgtagcta tgtcaatccg atcatgccaa tctactcggt gggtaagctt gccagtattg
    11221 agtataaaaa agccagtgcg ccaaaagata ccatttatca cgccaaagac gcggtacaag
    11281 caaccaagcc tgatatgcgt aagccacgcc tagtggtgtt cgtcgtcggt gagacggcac
    11341 gcgccgatca tgtcagcttc aatggctatg agcgcgatac tttcccacag cttgccaaga
    11401 tcgatggcgt gaccaatttt agcaatgtca catcgtgcgg cacatcgacg gcgtattctg
    11461 tgccgtgtat gttcagctat ctgggcgcgg atgagtatga tgtcgatacc gccaaatacc
    11521 aagaaaatgt gctggatacg ctggatcgct tgggcgtaag tatcttgtgg cgtgataata
    11581 attcggactc aaaaggcgtg atggataagc tgccaaaagc gcaatttgcc gattataaat
    11641 ccgcgaccaa caacgccatc tgcaacacca atccttataa cgaatgccgc gatgtcggta
    11701 tgctcgttgg cttagatgac tttgtcgctg ccaataacgg caaagatatg ctgatcatgc
    11761 tgcaccaaat gggcaatcac gggcctgcgt attttaagcg atatgatgaa aagtttgcca
    11821 aattcacgcc agtgtgtgaa ggtaatgagc ttgccaagtg cgaacatcag tccttgatca
    11881 atgcttatga caatgccttg cttgccaccg atgatttcat cgctcaaagt atccagtggc
    11941 tgcagacgca cagcaatgcc tatgatgtct caatgctgta tgtcagcgat catggcgaaa
    12001 gtctgggtga gaacggtgtc tatctacatg gtatgccaaa tgcctttgca ccaaaagaac
    12061 agcgcagtgt gcctgcattt ttctggacgg ataagcaaac tggcatcacg ccaatggcaa
    12121 ccgataccgt cctgacccat gacgcgatca cgccgacatt attaaagctg tttgatgtca
    12181 ccgcggacaa agtcaaagac cgcaccgcat tcatccgctg atttctccct gtattttttc
    12241 caaacccacc gcacactcca ttcgtattat gggcggtggg gtggggtttg ttatgccgta
    12301 tttatcaaat aaacgcctac ttgctgagat gagtatcgct cttgtcatgg cgatcgttgc
    12361 cacgctgacc cttgagcaca gtcagattaa tctgatggtc gctgattggt tttatctggg
    12421 tatggggcat tggatggttg ccaagcaagc ttttttgcca gatttgctac tgtattctgg
    12481 actaaaaaag ctgctgatgg cgatgctgat ctacttgctg gttgcgacca tttgccgtgc
    12541 ttatcatgag aaaaagggca atgctatcac tgccaagtgg cttgtcccag tgacaaaatt
    12601 tcgcgtgcgt gagcttgcgt atctggtgct gactttgatc ctagtgccga cagttgtcgc
    12661 gtcattgaag gcatatactc atgtggtctg ccctgtgcat ttgacgattt ttgatggtac
    12721 gctgccgtat ttgccgatgc ttgatagtat gcgtaacacc attcctgata agtgctttcc
    12781 tgcggcgcat gccagtagcg gatttgcgct gtttgccttt gcgtttgcgc caagtttgcg
    12841 ccgccgtcgt ggtgcgatca tcatcgtggt gatggcattg ggctgggcga tgggctgcta
    12901 taagatgatt attggcgatc attttttgag ccatacggtg gtgtcgatga tgcttgcgtg
    12961 ggcgatgtcg gcagggcttg cgtgggtgtt ttttaagaag ggtgaacaag tttaatcggc
    13021 tttaaaagta aaaacggtcg tatcggttat tacggaacgg ttttttcagt tgagtttcaa
    13081 agcgatgatt catcaacctg gaaactacca ataggaacgg ctttacttac gcctatcggt
    13141 caagaattga tgaaaatatg cggttccact ccagatattg cgtatttaaa taaattcctc
    13201 aacaaaataa atgttgaagg aagccaggta aaactatcaa ttattaatat gtaaagaggt
    13261 cgagttacct gtctgagttc cactaaccat aattaaaagc gaccgcaaaa actgcccagt
    13321 gaaaactgtg ttgccagtaa tcacaaatgc agcaagtact aataaagagc ctgttaagcc
    13381 cgcaaatatg tgggcttatt tttaattact cttcatcatc ccgcaggaac tgacaaaaat
    13441 cttcgctccc catcatatcg acagtgtttt taaattcgaa aaaagaaatt cctgtcatag
    13501 cacacacgcg ctggataatc agttttttct tttgctgtct gacggttgcg gtttcaccaa
    13561 actggataac agccaggact gtttcatcat atgatttcag gacaaaatca caggttatat
    13621 tctgcacttc acggattaaa tcttttacag cttcctcgct accatgagga cgaacaaatt
    13681 caatgagccg tattttggga aaaacggaat aatcaggtat aatattacgt gaaagaatgc
    13741 ctaaaaactc tgattcatta tttgagagta agggtacttt agtaaaacaa tcaacctccc
    13801 caatggctgt aaaagcattg gttgtcaaag ctggattgtt cttaataaaa tcagagaata
    13861 cattttcagc tgatggcaaa acaacagcag aattatgttg ttcatctcgt tttttattac
    13921 ggttaaatat catttcatat tccttttaaa agagcctgcg gcaggcacag gcaaaaacat
    13981 cgtttttgga caatctgatt caaatgttaa catttgacat cggattgtct atcctgcatt
    14041 gttctttact gtctgtaaga tcggcgctca ttcttccggc gttctcccga taccaaaaag
    14101 acggaacgca aaacggcttt cgatccacca ttcaaaaatc tgctctgcgc aaccacaaac
    14161 agttaaggcg atccatgcag caagaacaac gtaactactg acatctgcac ccatgtacca
    14221 ggcgatagtt aaacccatca ctgcgatacc gcctaaaaac tgcaacaaga aacgaaatcc
    14281 cgcaagaatc cccaatggaa taacaacaac cagataaagg gaagcagcca gaaggtatct
    14341 gacaggtgtc agcagataaa agaacctgga tattttcttt ttgtcagaat aaacggcatc
    14401 atttgcaaca tcggttgaat gatcattcat gctgtccctg attctttgaa aatctacgat
    14461 attatccttc atattgttat gacctcaatt ttcaggtggg tacattttat caaacatatt
    14521 agtaaactct gagtcttccc tggtatcgcc ctcataacca attgtataag agaactttaa
    14581 tttataaact tttctcccaa ttttttctga aactacgaaa cttaagttct ttacttccgt
    14641 atgtttcatg atttctttta ctgatttatt aagaacatct cttttaaaag caggataatc
    14701 tggatactta taatccttca ccccccccgc ccctattgta taaagattca attcatcttt
    14761 aagttcatct attgtcattt caaaactatt cgcgcgagaa ttattactat atattttacg
    14821 aatgagttga tatagtgatg cagcattaac acttgtcagt cgaacaacag ataataaaac
    14881 ttgcgttgta taacgttttt cttcaccaat aagtttacat aaaaatcttt tcgcttcttt
    14941 cgtaaacttg atccccactc taccttcatt cctgtaataa gtagaaaaaa cagtcagact
    15001 caaattcatt gaatcaggta cattactttt agtgaactta aatcccaaat cactactcag
    15061 ttctaatatt tcatcatgtt tcagcttaag aagcgtcgta tctaatatat tagagccatc
    15121 cctcaaagca agataagccg catcaggctt aacctgaacc catttaatgt aatcggcaac
    15181 tgtcacataa aatatgtgat catcatcgaa ttcattttta gagtttatct ggcacatggc
    15241 taaaaataag acttttttag cagccatagg caatgttgac agcgttgaat tcaattcatt
    15301 tcgatgtctc acttttgtta agcgagaaag ggaagtctta tttgtcgtca ttctcattgt
    15361 tccaacactc ttgataatca gactttaaca ccagaaacat gatattacaa cacaatcaat
    15421 atcatgttta accgagtatc ttctcatgtt tcatatctgt gatttatctc acaaaggtaa
    15481 tccatgctca aaatccacac tggattcaag gcagattaac aacagattca aaatagattc
    15541 accagaaaga acaacgaatt agtattctta tgacacattt tgtggatatc accgaggaag
    15601 ctctcatgtt taaccgagga agctctcatg tttaaccgag gaagctctca tgtttaaccg
    15661 aggaagctct catgtttaac cgaggaagtt atcatgttta accgaggaag ctctcatgtt
    15721 tagacaaaaa acacgcttaa aatcaataaa ttagagctac tctaagtttt taagttttta
    15781 agaaaaacaa gaaaagataa ggcttttaat gcgtgcgaat attcaggaac tgtggataac
    15841 tcaacaaaac cagcaacgat gatggttcct taccataaat ctgtcccctg atgattatta
    15901 cccgcaagct aaaacaaaca tcatcaatca ttaaaactca taactttcaa taacataaaa
    15961 tcatcaaaaa aatatttgat gccttatgta tctcaatttt caagaccata aatttgtccc
    16021 tagttaactt taacttattg aaataaatat aatattcgtg cgtactgatc ctaatgtcac
    16081 atcgcaaaaa ttccgcttcc tcgctcactc agtcgctacg ctcctgccgt tcggctgcgg
    16141 cgagcggtat ctcctttctc aaaaccagag agattcagat catatgatcc gttatttgat
    16201 cctttttgga tcttccacta agaagccctg aatcgcgctc tgccgcttcg ttcgatacaa
    16261 ccatgaggaa aaccatgcga aatgaaaaaa atcgcttaaa atgcgattca gagcgtttta
    16321 gggaggttct gttagttctc ctgcattgcg cccgtagttc gctaacgctc acacgcgcag
    16381 tacagcgccc cgccatgctg caatgcacca gggcggggta aactcgcttg cgctcgcgct
    16441 aactgcggca ttctgcggcg caaatcagca taatcatggc ctgctggtca gttgggcttt
    16501 gcagggtgaa gggatggcct gtgcaccctg gtacgttcgt cagcggctgc gccgcgcttc
    16561 ggggcgatgt ggctcctgtg ctgccctggt attttcgttc gccccttcca tccgcttacg
    16621 cggattaaaa aacgtgataa caatcactta tcgagtaaaa aaacgggaga aaacttgcaa
    16681 atacgactta aaggtcgtat tattgtttca tcgaaagggg aatggcccct ctcacaatag
    16741 cccagcaggg caggagatta aaaaaatgaa tatccaggaa gcattaaacg tttttggatt
    16801 atccggcgaa ttaactgaaa aagatatcaa agcagcatac agaaaagccg ctttaaaata
    16861 tcatccagat cgtaacccgt taggggctga actgatgaaa gcggtaaatg cagcttttga
    16921 tgtattgatg gcaaatattg ataaaataaa tcagttccag agcactgatg aacatgcacg
    16981 atataattac ggtgatgacc tggaaaaagt attaaacgtt ctttctggtt tatctggtct
    17041 ggtatttgaa gtgataggta actgggtctg gattagtgga gaaaccatta cacataagga
    17101 aactttaaaa gaaatcgggt gtaaatgggc ggcaaagaaa aaacaatggt tttatcgtcc
    17161 agacgaacat aaaagttact ggaatcgtga agaacacacg atagaagaaa tccgcgcaaa
    17221 atacggtaca accggacagc gcagggcgac agggtggcaa cgcgtggaaa ccagagcgta
    17281 accagaacgg gggcgagaag cccccgatat tatagccagc ccgtcttttg gggcatcgaa
    17341 cccaaaaaga cgggaacagc agccacgtag caaaaaggga aacagatgaa ctcttttttt
    17401 gaacaatatc accctgtctt tgaagttgtc tgccgtatcc tggggaacgg ctggcgcgtg
    17461 aacaaacttg atgattgttc gtctcgtata aaactgacgt caccgcagtt taaaaattac
    17521 tctgtgcata ttcggatgga aaaagaccgc ttttctgtgg tggggagtgt ggatagtcgt
    17581 tcctggcgta gtccgcatca tgtttgcacg ttatccagaa aacggaatcc cgttgatatt
    17641 gcagccgata tcgagcgaaa aattctggta aatgcttcgc aggaggtgtt acaggcgatt
    17701 gagtatgaga aacaccaggt ggaaaaaaag gacgaaattc tgatcctgaa aggtatgtta
    17761 tcgcaacttg tccagcttga aagctggtat ggggcattga caggctttaa agctgaaaac
    17821 ggattgaacg gtaaagtcac cgaacagggc gacagttatg atttacagat tcggggcttg
    17881 agtatagatc aacttgttaa aattacaggg tatttgaaac agttatgaga aaaaataaag
    17941 gtgatgtgac ttattttctt gaaaaagaag gtgacaatta caggcttaca aaaaggataa
    18001 aagccagaac aaatgttaaa atcgggaaca aaactacgaa aattacattg tatgatgctg
    18061 tactgaatga gaatgaatta caacatattg attttacgtg tgccggatta agggaagacg
    18121 atgagacgcc agttaagaat ttaattaagg agtttatgtt aaatgaaacc cgataataca
    18181 cctgaaaatg taaaaaaatt acgtttaaaa gcaggattaa cacaaaagga atgcagtcat
    18241 atttatggtg ttgggctaag aacctggcag aaaaaagagg aagttaatac tcaaaacagt
    18301 caaagtctgt cactggttga gtttgagttt cttttgttac ttgcaggaga acatcctgaa
    18361 tacgtgctct gcaaaaggga gtcaaaataa aatgacctgt gatggtttta tattagtacc
    18421 tggagttgcg ctcgtagctc gctgacgctc acacgcgcag tacagcgccc cgccatgctg
    18481 caatgcacca gggcggggta aactcgcttg cgctcgcgct aactgcggca ttctgcggcg
    18541 caaatcagca taatcatggc ctgctggtca gttgggcttt gcagggtgaa gggatggcct
    18601 gtgcaccctg gtacgttcgt cagcagctgc gccgcgcttc ggggcgatgt ggctcctgtg
    18661 ctgccctggt attttcgttc gccccttcca tccgcttacg cggattaaaa aacgtgataa
    18721 caatcactta tcgagtaaaa aaacgggaga aaacttgcaa atacgactta aaggtcgtat
    18781 tattgtttca tcgaaagggg aatggcccct ctcacaatag cccagcaggg caggagattt
    18841 aaaatgaaat gctcatctgt ttttacttca accactaatc atgttttcac attcgaacga
    18901 gtaacgcttt gcactattat ccttatgcat aaggatactg gacaacagta tgttgtcata
    18961 tttactgata acaataaaat tcgtgactat aaagccggaa ttgttcctca gtttggcgaa
    19021 cttaagcaga gtgatgttga tttagtcctt ttctacaggg acgaatatga aaaatatttt
    19081 gattcattaa aagatggcga tgaatgcttg agttttaaag attttattga gtgtctttgt
    19141 taagcattat gaatgcaggg gcatccctgc attttctgga aatttcaata ttgattctga
    19201 aattgtctat taacatcaat gcaagggaaa ttcatgaaaa cgttaaaaca agccgcaatg
    19261 cagtttgcat ctgatctgcg taataatcgc tgttttaaag ctgcttataa tgatgctctg
    19321 ttagagcttg atcgggaaga tttaaacgcc attgtacaat gcacatttct ttttgagcca
    19381 aaacgaacat tagccgaact tgataaatta attaaaaaga tagaagtaga agaagattat
    19441 gagttttgtg tatcccttga taaaggtaaa ggattacacg aagtcagaac atttaaaagt
    19501 gagcgtgagg cgttggatgc ttatcgcatt ttgactcttt acggatacac agtaactata
    19561 gaaaagaagg agggtaatga ctgactattc aaggtgtgat caggttgttc caccaacctg
    19621 atcacgttat cgacaatcaa aactgaatga ggtttttaaa tgtcaacgcg gcagatcgta
    19681 cagcttgctg attcccttgt caaaggctgg gaaaccagaa ttccggcaat gaaatttagc
    19741 gaatgggatc aattccggtg gtggttaaac tacttgcaag ggtatgaaat gttttaaata
    19801 aaaaaagggg ctttctgccc ctttgtattt tttgacaaaa atgccatgtg taactataat
    19861 tagttgcata tctatagggg atggatttat ggggaaaaca gataagctac tggcaaagtt
    19921 tttaaacagt aaaaaaacgt ttgaatggga tgaactggtc gttttgtttt cctctttggg
    19981 atatgtcaaa aaggaaatgc aggggtcaag agtgcggttt tttcaatgct gaaatcaatc
    20041 acaccatatt aatgcatcgt ccacatccgg aaagttatat caaaggtggt acgctgaaag
    20101 caattaaaca gaatctgaaa gaggctggat tattatgaaa catttaaaat ataaaggata
    20161 tttaggcacg gttgaacccg attttgaaaa taatgtcctg tatggaaaac tggcgtttat
    20221 tcgggacctg gtaacttatg aggcttctac attagctgaa ctggaacagg agtttaaaac
    20281 atctgttgat ctgtatttac agtcttgtgt ggaggatgga aaggaacctg ataccccgtt
    20341 taaaggcgtg tttaacgtca ggcttgatcc agaactgcat cgtcgggtag cagaaatggc
    20401 gatggaagaa gatttatcgt tgaatgcctt tgttaataag gctctggaaa aagaagtcag
    20461 caatcatcgc gcaggggctt aattgccctt gtttttatgc cctgtaaggg catgggaggc
    20521 gctttgcgcc ggaagccccg cagcgcagcg aggacttaaa aagcgttacg gcttcagccg
    20581 taacaattcc cagccctgcc aggcctgttt tttctttcac aaatctttca gctatacgtt
    20641 tgcctgatgg tcgaagacga aagaatcttt cagcaacgaa atctggacat atgcagcgac
    20701 gattttatag ccaggcacag tgccacatat ggtacaatca atgcaggata gacaatccga
    20761 tgtcaaatgt taacatttga atcagattgt ccaaaaacga tgtttttgcc tgtgcctgcc
    20821 gcaggctctg ttggggtgac ttatgaagat gatagcgttc aggctaagtg atgatgagtt
    20881 aaaatttgca gaacataacg ctattttaag tggatttacc agtatcaatg cttttgctaa
    20941 gcataacgtg cttaatattg aaaccaagcc tgttaacatc ccagttaata atgaaccagc
    21001 aaagctggta tcagtgcgtc tttaccctca tgaaatagaa cttgttaaac gtaatgctgc
    21061 tcttcacggc atgagcatga gcagagaaat tgctattcgt gtgcgtcagt ctttgttaaa
    21121 gagtgaagtc tgtctttacc ctgatgaagt taaggaactt aaaaaactat caactgctgt
    21181 agatcgtgtt ggtcgcaaca ttcattttat cataaaaggt gaaagattct gtacggttaa
    21241 cgatccagat ttcagaaaag acgttattga agtgattgaa ctgtgtaagc aaattgattc
    21301 aaaacttgaa acgttaacca agagtgttgt taacagattt gggtgattta tgggtgtgta
    21361 tgtagaacag gaataccgta ttaaacgcgc taaagggact gggcgtgatc cgaaatctcc
    21421 taagttatcg ggaagacata tacatgccag taaatcggct tttaatcata aagtcagaca
    21481 tggtgcggac aagaaagcat atacctggac acctacaaaa gaagtcactt ttaaaatcac
    21541 tggctcaggt aaaacagccg caggcatcaa aaatggtatt gattacatta cccgtaatgg
    21601 tgaacttgag gcttactgtt atgatggaaa aggttcagag caaacaggga aaggagaaga
    21661 ctttaatagg gagtttactt caactttatc taaaggaaat gattactcca ggacatatcg
    21721 tggagaaaac atcgatcatg tgaaaaacat ggtcttttca ccaccaccag aagctggagt
    21781 aagcagggaa gacgctctga aagctacggt agaatttctt aaagagactt atccaaacca
    21841 tgctttcgtt gccgtatacc atgatgataa agaagatcat cctcatgtac atgttaatat
    21901 taaacttcgt gatgaagaaa caggaagaag attaagactt actaaatctg aaaccaggaa
    21961 gttcagaaac ggttttcata gaaaactgaa aggtatggga tatgatgtta ccgctacctg
    22021 gaaaaaagat cccgaacgta aacgggagat tgagcgatta caggctgaaa atccgaaaag
    22081 actccggaat gtttacaagg tggtggattt tggggagact tcatatcaga ataaggcagg
    22141 ggaaaaacga acacctttcc tcacttatga aactttaaaa ggtggaaaac aggttacgat
    22201 ttggggtaaa gacctgaaaa atcatttcga gtctgaaaag ctccagccag gaacgcttat
    22261 caaagtaaaa aaactggctc ctacgcttgt acgtagtcca atgtttaatg atgatggaac
    22321 tgttgcaggc tacagggaaa cgcatcgcaa taactggcag attgaaaata ttgcccttga
    22381 gcggaacaga gaacgtcagg ttcatgagcg tgaaaccagg cagccggaag agagcgacgt
    22441 taaaaagcag ctatccagaa aacatgagca aggtcacaat atcgggtttg ctcttgaaca
    22501 cgggtttaca aaggattcag aagaacataa aaaactaaga atacagcaag agaggaactg
    22561 gaaaggcctg ggattttaat ttgattttgg cgcaggtgaa caaagcggct actttgctca
    22621 cctgctgatc acaacaacct acgagggagg ttatcatgac tgagcatgat gctatctgca
    22681 tttctggact tcatcaaatt tttagtgatg aagaacattt gagtgagcaa caaaaagaca
    22741 ttattctgat gtacgcttat ggctataccc tgaatgaaat tgctgatttc aaaggactaa
    22801 agccttcaac tgtcagaaaa tatctcgatt ctgtaagagc tgaactggga ggtgtctcgt
    22861 tggcagggat cagaacactg gtactcattc ggactaatgc ccttctcgtg tcaagtcttt
    22921 ccaggatcag tgagaggggg aatttatgaa aaaatatctg ctttttgctt tgccgttctt
    22981 cgttgttggt tgtagtgaag aagtaaaaag tgtggattgg tgggggcagc atcttacgga
    23041 agctaaacaa aagcaggcag agtgtgagaa gtctggctct gattctcaga actgtaagaa
    23101 tgttaaacaa gcattgttta ttcagtctca gaaggatgct ccagtcccta cattcgatta
    23161 actaatgttt caatcatact agagcgccca tatgggcgct tttttttgca tgatcacacg
    23221 aatcataata tgaccatttt gtcaatgtta tttgatcgta ttgttgagca ttgttatttt
    23281 gcgaatcatg ttcgcatcta aaacaaatcc aaaacacact tatagtggat tgattaaatg
    23341 aaggagaaat cagataagtc atcggaaaaa ggggtttatg tcacgataaa actcgatccc
    23401 aggttgaatg catttatcga agagaaaacg aaaacatctt tgcttagtaa gaggaagcaa
    23461 attatttact tattaatgca gagtatgcgt attgatggat atatggagcg ttagctatgg
    23521 cgtctttatt cccagccttg ttgctgtgtg cggcgtcagt tcatccggat acgataaatg
    23581 atattgcacg ggtcgagagt ggcttcaatc cttatgctgt ggctgaaatt attcctgaaa
    23641 gggaacgcgg tagctatggc aaaagtgtta tcagtcatat gcccaaaagt aaggaggaag
    23701 ctctaacagt tatcagagaa attgaaaaca gaaaaagacg ttattctgtc ggattgatgc
    23761 agattaccag cagtaatttt tctttttatt ccacgtccgc agaaaaactc cttgattcgt
    23821 gtgaaaactt gtctgttttc gaaaagatta tcgtggattg ttataaacga gggaggagcc
    23881 tggagaatgc gcttagttgt tactatacag gaaattttag taacggtaag cgaaaagaaa
    23941 aagaatttaa taatacaagt tatgttgagc gaattggtta tacagggaac gaaaaaaaat
    24001 atgttgtacc aggtacaaga agcaacgggg gagaacagcg aaaaaatcgt tcacacaatg
    24061 cgtcagttat ctggcccgaa accattctga aatctgcttt tgttgataac tcacatccaa
    24121 caaaagttat taattaacag gacttataaa atgtcattta agaaatcagt atgtaatcgt
    24181 atcagttcat ctgtcactgc tgtaatgcag cgtaaaaata aggtagttat ggcagtcgcc
    24241 ggggcaacag ttgcatcacc ggcctttgcg gatggatttt caaaagcgga aacactgctg
    24301 gaaaaggtta aaacagggct tagtggcctt tcactggtca cggtgactat tgcctgtctg
    24361 tgggtcggct acaaggttct gtttggtgga agcacgattc gtgaatgctc acctatcatc
    24421 atcggggcaa ttgttatcgc cagtgcggct gaagttgcat caatgatggt taactaataa
    24481 agcggggacg atagtatgtc tacactttat aaagcgatga cacgccctgc tatgtatgtg
    24541 ggtgttcctg tcgtccctct tactgtggtt gcaggggcgt tatttctggc gggggtttat
    24601 atcagcaagc tgatctggct ggctattcct gtcgcggttt ttgttctcag aatgatcact
    24661 aaacaggacg accacatttt taacctttac tttcttaagc tgaaaatgct cggaaactca
    24721 gtgtgtaatc gtttttttgg cgcacgagcg ttcctctccg gacaatatga agctgttgaa
    24781 attgatgagt ttgttaatgc catgaaactg aatgagcgaa tcactactgg taaatatata
    24841 ccctattcaa gccatgttga taaaaatatt gtcaaaacaa agaacggtga ttatgtagcg
    24901 acatggcaac tgatggggat taactttgag tcaatctctg cggagatgtt agaaactatt
    24961 gactcacagg ttgccactct tgttcgttca ttttccggac tgcccgtttc attttataac
    25021 cattcatgca gggcgagttt ttatgatgcc tttaccacaa aatcaggtaa taaatatgct
    25081 gatataatat cagattgtta ctatggttcg atgaagaaaa ataaattcaa ggggaacact
    25141 ctttacttta cgctgatcta cagaccagat gggcgtgtag aaaaactgga aaaacgcaaa
    25201 aaaagtatta aagaaaagaa agatgatatt aacattcatg tgaagagaat gaatgaaatg
    25261 attaatacat tctctggcgc tcttgataaa tttacctgta aacttcttgg tatgtatgag
    25321 gagaacggaa aggtattttc ttctcaactg tcattctata attatcttct gacagggaag
    25381 ttgcaaaaaa tcagagtcac tgattctccg gtgtataacg ttcttggtgg agtggatgta
    25441 ttttttaatc atgataccgg acagatatgc cgcattgatg gaaataaatt ctttcgttct
    25501 attgaaatta aagatttctg tagtgagact gcatccggtg tttttgatgt gctccagtat
    25561 tctgatgctg actatattat tacgcattca tatacatcca tgagcaaatc agaagcattg
    25621 agtaccatta aacgtgcgga aaaacagtta aagagtacag aagatgatgc ggttactcaa
    25681 ttgcaggaac tggaaaaggc aaaaaatgat attgtgtccg gtgatatatc ctttggttac
    25741 taccatttca cgctaatggt aatggcggac agtatcaggg agcttgacga atctgtaagt
    25801 aaaattacgg cagactttac tgacctgggc attattccgg cattgtcaac catgtcattg
    25861 ccagcggcat attttgccca acttccggcg gtttttcatt taaggccgcg tttatctccg
    25921 gtatcgaatg taaattttgt tgaactggca tcattccata atttctacca ggggaaacgc
    25981 gataaaaact gctggacaga agcggttgct attctcaaaa caccaagcaa acaggcgtat
    26041 tatctgaatt tacataactc agtactgttt aaagatgaaa caggtgaaaa gaatcttgct
    26101 aatactaaag ttataggaac ggcgggttca ggtaagacaa tgtttctgtc atatcttgcc
    26161 tgttcattac agaaatacaa taaccccgaa acttttgctg attctgcaaa aaataagaag
    26221 ttaacgtgtg tctttcttga taaggacaga ggggctgaat tatgtatccg aatgcttggt
    26281 ggggaatatt acaccgtaaa aagcggtgag ccgacaggat ggaatccttt tgctctggag
    26341 gcaacaaaac gtaaccgtat ttttgtcaag caactgatgg agattctgtg taccagaaat
    26401 ggtgaaagat tatcgacaag agaaagattg cttattagcg aaagtgttga tgctgtgatg
    26461 gattttcctc ctggcgaaat gcgggaatat ggcattacca gaatgcttga gcatcttatg
    26521 cagagagatg atcgggatga gcaggagaac gggattatcc ttcgtttgtc ccaatgggct
    26581 aatggtcagg ctcatggctg ggtttttgat aatgctaaag acacattcaa tattcagcat
    26641 gtcaataatt ttggcattga tggcaccgag tttcttgatg atccgatggt atgtgcacca
    26701 attactttct atctgctgta tcgcattacg cagcttctgg acggtcggcg tcttgttatc
    26761 ttcctggatg agttctggaa atggctacag gatgaagcat tcagcgactt tgtctataac
    26821 aagctgaaaa caattcgtaa gctgaacggg ctggttattc cggcaactca gtctccggat
    26881 gaaatactga agaataagat ttcacgggct gtggttgaag tatgcagcac cagtatttat
    26941 ctggctaacc ctgatgccga ttacaatgat tatgtggaag ggcttaagct gacaccagaa
    27001 gaatttaata ttgttaaaaa tcttgatcca atgtcgagac agttcctgat taaaaaaagc
    27061 agtcttaaaa agggggatgg taaatcattt tcagcacttg cgacactcga tttatcggga
    27121 cttggtggat atctgaaaat attgtctgcc agtgcagata atctggaaat ttttgaaagt
    27181 atttatcatg aaggaatgga gcctgatgac tgggttcctg aatatctcga acgggcaatc
    27241 tgacaggtga atttatgaaa cgtgtaaaaa cattgatgct gatatcatta ttgacggttt
    27301 cattttatag tagcgcagga attccggttg ctattgatgc cagtccggaa tgggctgttg
    27361 aagccgcaag atggacagaa cgactcaaac aatggtccga aactgcgcaa cattatcaga
    27421 gtcagattca ggcatataaa gaccagttag ccacggcaac aggtataagg aatattgcgg
    27481 cattcacaaa tgaactaagt aatctacaat cagaactgac aaatatttat aagcagggga
    27541 acagttatat ttctgatttt accagtaatc ccgaaggtgc attgagcagc caggctaaaa
    27601 atctgttcag caagtatgga gccttcgata tgtgtaatac gggctatgaa cgtaatgata
    27661 atctctgtaa agcacggatt gtgtcaacgg ctgccagtat tgaacaggga aatgagatca
    27721 ataagcagtt atcaagtgca atgtctcaga ttcagagttt aagtagccga attgaggctt
    27781 caaaggatat caaagaatcc caggatttgg ctaacgcatt acaggcgcag tcgttaaaaa
    27841 tgcaggcgat aaaaatgcag tatgatgtct ggaataataa aaataaagca gatcatgaga
    27901 tgttggtcac acaagaacaa gaggccttta ttaaacagca gaaagaagct gaaccattaa
    27961 cttttgatta aaaggtggat atatggctca ggggtttttc gttaaatata attctaccgt
    28021 tatggattcg gtagataaaa tcagctccag ttatcaaaca caatttgcta atgatattat
    28081 gtcgttagca acggtatcgg ttacgttata tgtactatgg aaaggatatc aaatacttgc
    28141 cagtaaaact cagacgccct tgcaggattt agtatgggat ctatcaaaat ttgcaatcat
    28201 aatcatgttt ataactaatg cggatgggta tttaactgcg gctactgatg cgttgcaagg
    28261 catgaaagat ggtttttctg gaggggtgag tgtctggcaa actttagata acttatggaa
    28321 aagtacgcaa aatcttggtg cggaaatata ctctttagat aaatcaacat atgttaaaga
    28381 tcaaggtgta gttggtcagt ttttaatatg gactggctcg cttattttaa tggctgttag
    28441 tgtggttgtt tttttaacag cagatgtaac gatgaaactc ctgattatta cagcaccgat
    28501 atttatcttt tgtctcatgt ttggttttat tcggagcatg tttaataatt ggttacaatc
    28561 gttattctcc agtattctga cagttctatt tgccagttta gtgattcgga tagcaatgga
    28621 ttttcaaggg atgatccttt cccatgctat tcgtgctgca caaacaggga atgtaaatct
    28681 tgtatctaca ggtgctatgg gctttatggc tgggtttttg ggggctttat tggttcttat
    28741 agctaaaggt tttgctgtcc agttagcagg tgcaggtgtt gaaggtgctg ttcagggcgc
    28801 agccatgatg gggctgggtg ctgctggtat ggctactggt aagtctctga tgcttggcgg
    28861 tcgtgctggt ttaggttttg gcttagggat ggccggaaga acgggcatga attctttatc
    28921 agggaaagca gggaatctga tggggcgtgg tgcgcgaaca gccgctgaat gggcgggtga
    28981 aaagggatat caggcgctgg ctccgacagg tgctttgggg atgaaggcga gaagacttgc
    29041 atccctggaa aaagcacgag cgaggaacgc cgcatgaaaa taatgaaacg ttttttttat
    29101 gtgatgtccc tgaactggat actggtgttc gttgataaac atatcacctc cggatggagc
    29161 aggaagagaa agctaataac ccagcttgtt attttaatca cgggaattac cttatttaag
    29221 gtcttaggtg gaatcagtca gagctggttg tgacatgctt cattattttg tcatgatact
    29281 ttattttgtc ctaatccttc ttttaggagt gtttacctgc ttcgcaggaa taagacttgg
    29341 tttagggctt gcggggaaat ggagaaaggg tatggatctt cgctttttgc ttcttattgg
    29401 tgggattctt tttcttcatc ttggccttta tctcttaaag gtgttcgtga acgcttaata
    29461 aggtgaattt atgcgatgtg tgattctttt gtttttcctg tttcagattt ccggatgtac
    29521 cacaaggaat aaccttcctt ctgatgtttc cggcgaactg gaacccatta accacagcca
    29581 ggtaatcagt tatgagtgaa aaaatcgaaa agaagattga gacagctaaa tccttcgaaa
    29641 gacaactcta tgcagaaaat gaacggtcta agaaaaatgc atggcgtgtt gcagtggcag
    29701 catcattact gtccttatgt cttggggctg caatttgtgt tcttgtgcca ttaaaagaaa
    29761 aagagcttgc tattgtggct gttggcgaaa aaacaggccg tacagaactg attactcagg
    29821 ttaaacagga aaagatcctg caatctgaag cacttgggcg gtattttgta aacacgtata
    29881 ttactttacg tgaaggttat aattatccgt ctcttcagta tgattatgag actgtacagc
    29941 tatatagctc gaatacagta aaggacgatt atcttcgtct gtataacagc gatatggctc
    30001 ctgataaaat ttatcataat aacggttctt acgttgcggt tgaggtcatc tccaatatta
    30061 tttctgacgc aaccgcgcca gataaactgg cctctgtgcg ctttaaaaaa acgacgagaa
    30121 acttcacgac cggacaggtt tcagtttcat actggacggc tcgtgtgaca tatcgttttg
    30181 aaccagaaaa gtcggttaaa agttccagtc gtgagcttaa tcctctcggt tttactgtaa
    30241 ccagttacca gacagatcgg gaagtcaggg gggaatgatg aagaaattat ttattgctac
    30301 atgtttattg cttcctgggc ttgtgtattc agcagcgact ccgttacctt ctggatttga
    30361 tgcaaggatg cagactgtga gttataacgg tgcgaatacg accgttatcc ggtcaaagac
    30421 aggatttctc acatcagttg tttttgatga aggtgaggct gttatcagtg caaaggctgg
    30481 ttttcctgcc ggatgggaaa tcacaaccga tgataatgtt gtttacatca atccgcgtcc
    30541 cgttgttcag gaacaggaag gcgatgaagg cgaaaaactg aaaaaggttt tccagccaac
    30601 agaaaaggaa tgggatacga atctttttgt cagaacaaca aagcggattt acagtctgga
    30661 tctaatcctt ctgtcagaag aaaaacaagc gcaacctgct tatgttgtac aattccgcta
    30721 tccatcagaa atagcgaaaa agaatgcaga agaagtaagg ctggcaaaag aaaaacagga
    30781 aaaactcaga cagaagaaac tgatttctga aagttttgaa aaagccgatg cgcctaaaaa
    30841 ctggaattat ttcatgcgag tcaatgaaaa atatgacagt cgtcgtatcg cccctgattt
    30901 tgcttatgat aatggcatat ttaccttttt aggttttaac tcagggaaag tattcccagc
    30961 tccatttgct gtaagagatg gtcaggaaca gacacttgct tttaatgtgg aaacaaaagg
    31021 caagtataaa attatggtta tccacaacgt taatgacaaa tttgttttac gctatggaaa
    31081 cagcgttgtt ggtgttgtga ataaatcttt cggaaaagta ttaaccgacc agaggaatac
    31141 atcttctcct gcggtagaac gtgttgaggt gaataatgac tgatatgaaa aaaacagatg
    31201 atttagttga tgatgcgaat cagtcaaagc tggaaagtaa acccgttcat gatatcggtg
    31261 atatcaggaa caaaaataat cgtatgcgtt ctggctttct gtttggtctg atggctgtat
    31321 tagttattgg tatttttgcg ctgaaaacat ataaaaatta ttttgcagat gaccagcaga
    31381 aagcatcaga atcaacaggc gataccagca tttctcaggt aagtaaaatc cgtactggtc
    31441 ttggtcagaa ttttgaccct gttgagaaca aagcaattat caatccaggc gctactggtt
    31501 ctggtaatac tgtttctgac ggtcataaag aagtgccaca ggaggggttc aggaaatatt
    31561 tgtcaatacc tgttgctggt cagggaggga gtcagactgg cgcgagtggc tgcagcagga
    31621 catcgcagac atcagagcca caggaagaaa ggaaagaatc aaaagaaaat gtgcctggta
    31681 aatcaggaat gaaagtaact gcgattaatc tcgatcctga tctctatatt gaggaaaaca
    31741 ggcttatacc gtgtgcttta accacacgct ttgtttctga tgttgcaggt cgtatcagtt
    31801 gtgtttttac agaagatgta tggagtgcta atcaccacac aaaattactg gaacagggaa
    31861 ctaaggcttt tggtcgttac cagaccggaa cgctgaatca tggacagggc agaatgttcg
    31921 ttatgtggga gcagttgcgg acgcctgata ataaacgaat cgatatggta aatacagcag
    31981 cagctggtcc cctgggagaa gcgggcattg atggatggat cgattctcac ttctgggagc
    32041 gttttggtgg ttctcttatg ctcagtatgg ttcaggatgt agcggcggcg gcggcggata
    32101 atgcacctgg taaagatcgt aatgtggatt ataccgaaaa ctcacgacag gcaatggctg
    32161 aaatggctaa agtcgcactt gagaattcga taaatattcc tccaacaatg tataaaaacc
    32221 agggtgacat aatcactatt atggtcgggg aagacattga tttttctgat atctatgagt
    32281 taaaggtaaa atagtatgag tggcgttatt cctgtcagca ttagcaataa atcgctcgat
    32341 ttttataaaa atcaagtatt ccagtctttt cttgatattg aagggctaac agaaattgct
    32401 gtcaaccgtc ctggtgaaat ctggacggaa attaatggtg actggacatt ccataataat
    32461 gacagtgtga cctttgattt ttgtaaacgt ttttctcata cgctggcatc atttcgggga
    32521 gatgaaatcg gcgagaccaa gccattactt tcagcaacac tggaatcagg agaacgtgta
    32581 caggtcgttt ttcctcctgc atgtgagaga aatactattt caatcactat acgtaaacca
    32641 tcgacacggc agataacaca taaagaatat gtggcgaacg gtttttatga ttatgtccag
    32701 tcaggaaaaa aacacaggac atacgatgat gagcttcttg atttattcag agcgagaaat
    32761 atcgctgctt ttatggagct tgccgtagca gcagggaaaa ccattgtatt tgccggagca
    32821 acgggttccg gtaaaacaac ttatatgaag agtcttatcg atttcatacc attgaataca
    32881 cggctgatta cgattgaaga tgcagaggaa atcaaatttt ttatccataa aaattatgtg
    32941 catctttttt acccttcgga atccggcagt gattccggct ctatcataac agcagcgaaa
    33001 ttaatcaaat cctgtttgag gatgaaacct gacagaattt tactggcgga aattaaaggt
    33061 ggcgatgcct gggattttgt gaaagttgct ggttctggtc acagtggaag catgacgtcc
    33121 attcacgcgg cctcggcaaa agatgccatc attcagatgg tgacaaaatg ttatcagaac
    33181 agagaatgtc agaatcttcc ttttgatgtt cttcgtaaaa tcattatgga cagtattgat
    33241 attgttgttc atgtaggcag ggatggtgtt gtcaggcata tgagcgacat ctattacaaa
    33301 ggggctgaat
//
