LOCUS       NZ_CP117663             6230 bp    DNA     circular CON 25-MAR-2024
DEFINITION  Escherichia albertii strain BIA_13 plasmid pEA13_4, complete
            sequence.
ACCESSION   NZ_CP117663
VERSION     NZ_CP117663.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33094093
            Assembly: GCF_028622395.1
KEYWORDS    RefSeq.
SOURCE      Escherichia albertii
  ORGANISM  Escherichia albertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Leszczynska,K., Swiecicka,I., Daniluk,T., Lebensztejn,D.,
            Chmielewska,S., Leszczynska,D., Gawor,J. and Kliber,M.
  TITLE     Escherichia albertii as a potential enteropathogen in the light of
            epidemiological and genomic studies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Leszczynska,K., Swiecicka,I., Daniluk,T., Lebensztejn,D.,
            Chmielewska,S., Leszczynska,D., Gawor,J. and Kliber,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2023) Department of Microbiology, Medical
            University of Bialystok, 2C Mickiewicza Street, Bialystok, Podlasie
            15-222, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. v1.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 287x
            Sequencing Technology :: Illumina Miseq/Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028622395.1-RS_2024_03_25
            Annotation Date                   :: 03/25/2024 01:44:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,085
            CDSs (total)                      :: 4,959
            Genes (coding)                    :: 4,714
            CDSs (with protein)               :: 4,714
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 100 of 245
            Pseudo Genes (incomplete)         :: 174 of 245
            Pseudo Genes (internal stop)      :: 45 of 245
            Pseudo Genes (multiple problems)  :: 62 of 245
            Pseudo Genes (short protein)      :: 3 of 245
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117663.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Escherichia albertii"
                     /mol_type="genomic DNA"
                     /strain="BIA_13"
                     /host="Grus grus"
                     /db_xref="taxon:208962"
                     /plasmid="pEA13_4"
                     /geo_loc_name="Poland: North-East"
                     /collection_date="2015"
     gene            complement(join(5632..6230,1..1129))
                     /locus_tag="PS034_RS25250"
                     /old_locus_tag="PS034_25250"
     CDS             complement(join(5632..6230,1..1129))
                     /locus_tag="PS034_RS25250"
                     /old_locus_tag="PS034_25250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_000012953.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENNP
                     WGGGSGSGVHWGGGSGNGNGGGNGNSGGGSNSSVAAVAFGFPALATPGAGTLGIAVSGE
                     ALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVTNVQV
                     STLPLDQATVSVTKRVTDVVKDTRQHIAVVAGVPMSVPVVNAKPTRTPGVFHASFPGVP
                     SLTVSTVKGLPVSTTLPRGITEDKGRTAVPAGFTFGGGSHEAVIRFPKESGQKPIYVSV
                     TDVLTPAQVKQRQDEENRLQQEWNDTHPVEVAERNYEQARAELDQANKDVARNQERQAK
                     AVQVYNSRKSELDAANKTLADAKAEIKQFERFAREPMAAGHRMWQMAGLKAQRAQTDVN
                     NKKAAFDAAAKDKSDADAALGAALERRKQKENKEKDSKDKLDKESKRNKPGKATGKGKP
                     VGDKWLDDAGKDSGAPIPDRIADKLRDKEFKSFDDFRKKFWEEVSKDPELSKQFKDSNK
                     TNIQKGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMDNIRVTTPKRHIDIHRGK"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(1350..1883)
                     /locus_tag="PS034_RS25255"
                     /old_locus_tag="PS034_25255"
     CDS             complement(1350..1883)
                     /locus_tag="PS034_RS25255"
                     /old_locus_tag="PS034_25255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(1886..2812)
                     /locus_tag="PS034_RS25260"
                     /old_locus_tag="PS034_25260"
     CDS             complement(1886..2812)
                     /locus_tag="PS034_RS25260"
                     /old_locus_tag="PS034_25260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001447301.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIEGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFETAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREYGFD
                     MRDGVSRQERTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            2989..3387
                     /locus_tag="PS034_RS25265"
                     /old_locus_tag="PS034_25265"
     CDS             2989..3387
                     /locus_tag="PS034_RS25265"
                     /old_locus_tag="PS034_25265"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_009449624.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQENERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(3648..4667)
                     /locus_tag="PS034_RS25270"
                     /old_locus_tag="PS034_25270"
     CDS             complement(3648..4667)
                     /locus_tag="PS034_RS25270"
                     /old_locus_tag="PS034_25270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271349.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKC"
                     /name="replication initiation protein"
     gene            complement(5164..5307)
                     /locus_tag="PS034_RS25275"
                     /old_locus_tag="PS034_25275"
     CDS             complement(5164..5307)
                     /locus_tag="PS034_RS25275"
                     /old_locus_tag="PS034_25275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001314446.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            complement(5369..5629)
                     /locus_tag="PS034_RS25280"
                     /old_locus_tag="PS034_25280"
     CDS             complement(5369..5629)
                     /locus_tag="PS034_RS25280"
                     /old_locus_tag="PS034_25280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000420692.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLTE
                     HPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKPGFKQG"
                     /name="bacteriocin immunity protein"
     BGC             1..6129
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(3893..4667)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(1890..2812)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.403"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttggttctcg ggcaaatcgc tcgaattgtt ttatttcagc ctttgcatca gcaagagttt
       61 tattcgcagc atcaagttca cttttacgag aattataaac ctgaacagct ttagcctgtc
      121 gttcctggtt tctggcaaca tctttattag cctgatccag ttctgcgcgg gcctgttcat
      181 aattgcgttc agccacttct acaggatgtg tgtcattcca ttcctgctgg aggcgatttt
      241 cctcatcctg acgttgtttt acctgtgcag gggtaagaac atctgtcact gatacataaa
      301 tcggcttctg cccactttct ttcgggaaac ggatcaccgc ttcatgtgaa ccaccaccaa
      361 aggtaaatcc tgcaggaacg gcagtccggc ctttatcctc cgtaatacca cggggaaggg
      421 ttgttgacac cggaaggcct ttgacagtgc tgactgtcag agaagggaca ccaggaaatg
      481 atgcgtgaaa tacacctgga gtacgtgttg gtttggcatt cacaacaggc acactcatag
      541 gcacacctgc aactacggca atatgctgtc gtgtgtcctt cacaacatct gtcacacgtt
      601 tcgtcacgct gacagttgcc tggtccagcg gaagtgtact gacctgaaca ttcgtcactg
      661 tttctgccgg taatgacgtc acaatctttg acatcatatt cgggtcatct tttgctattt
      721 cagatggcag aatgccgtaa agcgcaatac cccatgcgct gaatttaaac ggacctttca
      781 gggctgcaaa gatatcagct attgccgcag ataaagcctc accagagacg gcgataccca
      841 gcgttccggc accaggagtt gccaaagcag gaaaaccaaa tgccactgct gctacggatg
      901 aattgctgcc accaccggaa ttaccattcc ccccgccatt accattgccg gagccacctc
      961 cccagtgaac accactaccg gaaccgccac cccatggatt attttccgaa ctccatcctg
     1021 aaccgtcaga agccccacca tttccaccaa gccccgtagg gccgccatta atgttaccac
     1081 ctgtgttatg tgcgccactg ttgtggtcgc gtccatctcc accgctcata aaaattccct
     1141 cttttaagcg ttaaaacaat tgattacata gttaatactg tacataaaac cactggtttt
     1201 atgtacagat caaaaagtac tcgccattat tttttctgtc aagtcagggg gggatatttt
     1261 ttggcagttc ggaggaatca ctcatgaaaa cggtgaacgg aacgcgaaat cctttcgcgg
     1321 gtcgtgaggc gttggacaag cgaagcgcgt cagtttttcc ggctcttacc cgtgacaggt
     1381 ttctccacca gtttatcttc cagtgtcaca actttctgat acaccgccgc tgtggcgttg
     1441 tagttcctgt cgagtattgt tttcattccc catccgagca cacagaacat aaccagaaaa
     1501 gccactgaca ccccacccag ccatttccag ctataccctt taagggtgtt gtattcctgc
     1561 acgatattgc tgtgaattgt cgcaatatcc ctgttcaggg catccgcgtt tgccttcatg
     1621 tttgcgactg actcttccag ccgctgaaaa tcctggctga tgcgctcacc tatcagtcct
     1681 gccacgtcca gcttctgcac ctcatttctg acttcatccc tgacctgttt tgccacaccg
     1741 tcagaaatat cccgatatcc gcgaagtgtt tcccttgcca cagccagaga atcagcaaca
     1801 gctttctgtt gctccattgc ggtatgttgc agatgctcca ccgataacag gagcgtgagt
     1861 aatttttctt ctgtgctggc catgttcaga atcccataga aaatccacgt gacggtgatt
     1921 taacccgttc aggttcagtt cgctcctgtc tgctcactcc gtctctcatg tcaaagccat
     1981 actcccgcgc aacatcatgt tgcagccttc tgaccagttc atgttgctta accggagtgt
     2041 ccagccattc cctgatgtca tgcccgaccg ttcgcgcctg ttcaagatgg cgggaatagt
     2101 ccggttcaga ccgtttttgc atgatgtcat gacgctgggc atcatcaagc cgtccggcag
     2161 tttcaaaacc cagatgcact tcaggacgtc gttcaatgcc ctgttctttc agcgtccggc
     2221 agtcaatgaa gcccctttca aagccatgct ttctcatatg ttcgttgtgc ttcacctccc
     2281 agcgttttcg caattcccgc aactgctctt tttgctgagt gggagtcagg cttcctgaat
     2341 cctttttcgc acctccccgc tcgggatacc tggcgttata gcgccggaaa tactgttccg
     2401 gtgtacgttc aatgccatca ttcacacgct gactcatcat gatatgcgca tgaggctgtt
     2461 cgcctccctc aatgctcgct ttcggattgt gaatggcaaa actccatgca tgccgttcgc
     2521 cagcttcctg acgtacaaaa tcctgcacca gttcaaggcg ctgttccggt gtcagttcac
     2581 ggggcagcgc tatctccagt tcccgataag tcgaaccgtt tgcacgttca aactggtcag
     2641 ccgcctgcca gaaatgtgag ggattatctc gcgcccattc cggcatgttt ccgtgtgcgg
     2701 aatattccaa atcctgacgg tcgcggtatt tctctttgcg ctcgatgtaa tcggcgtggt
     2761 ttgcagcctg ccccttgcca ccaaatttaa cgctgaggtg ataggttgcc acggtttcag
     2821 ccctccgcag gagcgcactg tttctcgacg aaggaaagaa acatataagt gcgcttcctt
     2881 ctaattttac gaattagcac ggaagatacc ccctgcaccc gccgaccgca agcgatatac
     2941 tgcacgacca caacacaccg caaaaaaaca ccagggagca ccacgaacat gcgcatcagc
     3001 aacatcgaat ggctgaagaa acggatcgga ttcatcagga agctcggaga acagaccgca
     3061 cgacagagac agattattga cctgctcgac aacgaagcag gactcacaga acaggaacgc
     3121 aaactcctcc acgtactggc aacagcggaa aaaaacgact tacaggcaca ggagaacgaa
     3181 cgaaaacagg cggtccaaaa acgaatcgag ggaaaaaaac aacgacgaga acgcaatcac
     3241 cgactctttc tcgcggctgg gctactgatc gaagcaggac tggtagacac aaaaacaggc
     3301 gagctatgct ataaaaaaga caggatatta caggcactta aggaaattaa gtacgatctg
     3361 gagacatccc caaacccaga cgcgtaagtg cctccatccg tgttccggtt ccctggtgct
     3421 tcctcgctca ttcgctcgct acgctcggtc atgagactgc ggcgagcacg accacctcaa
     3481 tcatgcggta agccgcacca ttgctgggct gagaggagaa aaccgccgga aagcccgctg
     3541 ctcaaaaaga cgccgaaagg cgctgttatc tgatataggc ttgttcatgt ccgcgaattt
     3601 aacatcccat taacaaaaaa cggggggtag tgttgcttct ttgttaacta acatttaatt
     3661 atttgtttct tggttagcca ttctccagaa ttatccatcc agcgtcgaat agtccggctg
     3721 ctaacaccaa ccgccactgc aatatcttcc gttgacgctc ccaagatata catatcaagc
     3781 gccctacgac gtttgtcgtc gttagcagtc caccgaacag cggcgctttt tttccctttg
     3841 tccgactgat tttctgacga attggccttc ccgcccttgc gaccgcgtgc tgcctgaatt
     3901 tctggcgtgt gcgtatcagc cacatactgt gcgaaagttt ccggcgtgaa gttcctgtgc
     3961 gtgtatttcg caatgctctt gccaatagcc cgacattcag ccagtgaaag cggaacggga
     4021 agcgatgcat tgtacatttc gacacgctgg atcaccgctt caagccactg tgagaacgct
     4081 ggccagccct gacgaatcgc cctgtaagcc catttgcgcg tcatttcgaa caggtggcag
     4141 tttcgcccca tcccgtaatg tttatcgatg ctacgacgct ctgaggcgct caaatcgaga
     4201 taatctgcca gttcatcgag ggtataggcg tcctcgcgcc actccatcac ctgccattcc
     4261 aggtggaacg gatttttgca aatcaggccg ctgtagttca catcagcaca aagtttctca
     4321 cacagcgaac gttcaaccgc tgcggcatat ttcagcgcct taaccgaggc atcaggcgcg
     4381 gttctcacgg cgatgttgag tgcgtagagc aggtgagcgt gtccgttcac aggattttta
     4441 acggtgatgt tcggcgcggg ggcgttccgg tcgctccagt caatcgcagc cccctcgcgg
     4501 tcaacgtcaa aaaccagcca gaactgcgca tgaggctggt tctgctggat gtaccgcgca
     4561 agaagcgcac gccctttgcc ggagatgcga aggccgaaag cgagatcatc cgtgtgatag
     4621 ggacgatggg gtaaattttc gtcgaagtat tgaagcgcgg cgctcatgtg ttacgattct
     4681 ctccgggtta tgcgacctac acgccctctg tatttcatgt tgaatcttgc cggatctgga
     4741 catgatttca ggggcgtttg tttttcagtc gcttacacta cacccaacgg caaatcctgt
     4801 cgatccttcc cgcaaaaccg atccatttac cataactccc gttgtacgca ccccttaaaa
     4861 cgccctcagt cgctttctga cgcaaaaaac gcaaaaagac aagaaaacac caggccaaac
     4921 cagaaaaacg cgccagaatc gtttttagag ggctgcataa ctatgcacga aagcgatgcg
     4981 cgatcgattt cgactgttcc gaagggcgaa caggtcaaaa tgacaagttt tctcgccctg
     5041 ggtggaaagt tctgcacggg gattttagct ctcatctccc gcatggaatt tttgtgacat
     5101 ttcaaaaaaa agcgcatcag aatcagcctg acacgctttg tttttgtcaa agaggatttc
     5161 gggttactgc gttgctaatc cggtcacttc agctgttgat gacggggata cagtcccgcc
     5221 ctgaacatcc cggatatagt ttgcctgaca tgcagccaga ataatgactg caagaagcaa
     5281 taaaataatc cctgttattt ttttcataac gactccttgt tgtgaaagaa tgccagccat
     5341 tcatccataa acaaccggca ttcatatttt agccctgttt aaatcctggc ttaccgttag
     5401 cagctcgcca ttctttaatt tctttaacaa tcccttcagg actatcttcc ctgtcatcgc
     5461 gaggataata aatcagatct gaaccatctg ggtgctcagt taatcgctca aactctctca
     5521 ctaatttatt gtcatcctct tcagtagcac cttcagctct acatattttt tttacaaact
     5581 ccagaaattc agcctcggta taatcactaa tactatgttt cagttccatt tttacttacc
     5641 tctatgaata tcaatatgtc gcttaggtgt ggtcactctg atattatcca tatcatagac
     5701 accaccatcc tgactgattg gtttatcatg atgtaattca aagcgttccc taccacctac
     5761 ttggtctttc ttccttgcaa aaggtgcttt tcccttttga atgttcgtct tattactgtc
     5821 tttaaattgc ttactaagct cgggatcttt tgacacttct tcccagaatt tcttccggaa
     5881 atcgtcaaaa cttttaaatt ctttatcacg caacttatca gcaatgcgat caggaattgg
     5941 cgctcctgaa tctttacccg catcatcaag ccatttatca ccaactggtt ttcctttacc
     6001 cgtcgccttc cctggcttat tccgtttact ctccttatct aatttatcct tagagtcctt
     6061 ttctttattt tccttctgct tacggcgttc cagcgcagca cctaacgcag catcagcatc
     6121 agacttatct tttgcagcag catcaaatgc agccttctta ttatttacat ccgtctgtgc
     6181 ccgctgggcc ttaagccctg ccatttgcca cattcggtga ccagcagcca
//
