LOCUS       NZ_CP117633             4657 bp    DNA     circular CON 25-MAR-2024
DEFINITION  Escherichia albertii strain BIA_22 plasmid pEA22_5, complete
            sequence.
ACCESSION   NZ_CP117633
VERSION     NZ_CP117633.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33094099
            Assembly: GCF_028622595.1
KEYWORDS    RefSeq.
SOURCE      Escherichia albertii
  ORGANISM  Escherichia albertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Leszczynska,K., Swiecicka,I., Daniluk,T., Lebensztejn,D.,
            Chmielewska,S., Leszczynska,D., Gawor,J. and Kliber,M.
  TITLE     Escherichia albertii as a potential enteropathogen in the light of
            epidemiological and genomic studies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Leszczynska,K., Swiecicka,I., Daniluk,T., Lebensztejn,D.,
            Chmielewska,S., Leszczynska,D., Gawor,J. and Kliber,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2023) Department of Microbiology, Medical
            University of Bialystok, 2C Mickiewicza Street, Bialystok, Podlasie
            15-222, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. v1.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 303x
            Sequencing Technology :: Illumina Miseq/Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028622595.1-RS_2024_03_25
            Annotation Date                   :: 03/25/2024 01:46:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,342
            CDSs (total)                      :: 5,213
            Genes (coding)                    :: 4,876
            CDSs (with protein)               :: 4,876
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 93
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 337
            CDSs (without protein)            :: 337
            Pseudo Genes (ambiguous residues) :: 0 of 337
            Pseudo Genes (frameshifted)       :: 133 of 337
            Pseudo Genes (incomplete)         :: 229 of 337
            Pseudo Genes (internal stop)      :: 64 of 337
            Pseudo Genes (multiple problems)  :: 76 of 337
            Pseudo Genes (short protein)      :: 3 of 337
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117633.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Escherichia albertii"
                     /mol_type="genomic DNA"
                     /strain="BIA_22"
                     /host="Grus grus"
                     /db_xref="taxon:208962"
                     /plasmid="pEA22_5"
                     /geo_loc_name="Poland: North-East"
                     /collection_date="2015"
     gene            complement(join(4151..4657,1..261))
                     /locus_tag="PS041_RS26590"
                     /old_locus_tag="PS041_26590"
     CDS             complement(join(4151..4657,1..261))
                     /locus_tag="PS041_RS26590"
                     /old_locus_tag="PS041_26590"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_225568386.1"
                     /translation="MQPRELLMLSMYILVQAPVLTGLSKNQTESLTTNNKRGRRTVFYR
                     IKKHLQHDFYRIDSIKNEIHSPIKGFYMKDMKAVVVFTGKDLNIMRTEGGSGYWHARTD
                     RLNDADYLIAVRNRRETWAVKDLEHGTAFLIAKITGCFKSPDYDDRNVITFDEYAEIHT
                     PKAWKMLTDGQRYPVAYLSAQEAFLRIGVTPEQLEWKKFHPSSPSVPNTVIPGLAEEKT
                     EKLSLNEAIERAKKDISNATGIDSSAITISIKI"
                     /name="hypothetical protein"
     gene            521..1000
                     /locus_tag="PS041_RS26595"
                     /old_locus_tag="PS041_26595"
     CDS             521..1000
                     /locus_tag="PS041_RS26595"
                     /old_locus_tag="PS041_26595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001050742.1"
                     /translation="MNLLLPRDIVEAVLNDKKTKNARVAKCDGSEFFLELPSMNADFPA
                     GKIILKLGDSGFYNKRTKSLEGAYGLRHIWDKHRVEIGATSAEDIVIFLESILLAGAEV
                     LIDPKKGQNKAIVVESGTGMMILELKKPNGEDPYYSIITAYDRKSHPGTKLHTLI"
                     /name="hypothetical protein"
     gene            2247..2570
                     /locus_tag="PS041_RS26600"
                     /old_locus_tag="PS041_26600"
     CDS             2247..2570
                     /locus_tag="PS041_RS26600"
                     /old_locus_tag="PS041_26600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001347450.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSL
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2779..>3213
                     /locus_tag="PS041_RS26605"
                     /old_locus_tag="PS041_26605"
                     /pseudo=""
     CDS             2779..>3213
                     /locus_tag="PS041_RS26605"
                     /old_locus_tag="PS041_26605"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3248..3733
                     /locus_tag="PS041_RS26610"
                     /old_locus_tag="PS041_26610"
     CDS             3248..3733
                     /locus_tag="PS041_RS26610"
                     /old_locus_tag="PS041_26610"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001086854.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSAAMSQRTKGMLRMVSQTWLTIVLVSTLLTASGASILWWQGQQIL
                     DNYTTIREQKRTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            3737..3952
                     /locus_tag="PS041_RS26615"
                     /old_locus_tag="PS041_26615"
     CDS             3737..3952
                     /locus_tag="PS041_RS26615"
                     /old_locus_tag="PS041_26615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137421.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSEQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          1960..2143
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.561"
                     /coverage="86.0"
                     /evalue="2.7100000000000003e-47"
     Typing          complement(1501..1977)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.083"
                     /coverage="92.0"
                     /evalue="3.51e-174"
     Typing          2561..4056
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.984"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caaatccttc ccggtaaaaa ccacaacagc tttcatatct ttcatataga aacccttaat
       61 aggtgagtga atttcatttt ttatggaatc tatacgataa aaatcgtgtt gtaaatgttt
      121 ttttatgcga taaaatacag tcctcctacc tcttttattg ttagtggtaa ggctttctgt
      181 ttggtttttt gataaaccgg ttaaaacagg ggcttggacg aggatataca ttgaaagcat
      241 gagcaattca cgtggctgca tggctatgca gccacgtgaa atttatcagg cgcagaatgc
      301 gcggtttagc ggggagttta ctgccggttt taccgggcta acgccgcttt cacgctgaac
      361 ccgtccggag cggtgcgggc aaggaatgtt atggtaaata caactggttt tttctatcaa
      421 gatttactgg aaattgctca ttacgtcagt gttagaatca gcccttcaaa aagggattta
      481 acgcctaaac ataatagtga aggctataga ggtgtagttt gtgaacttgt tacttccacg
      541 cgatatagtt gaagctgttc ttaatgataa gaaaacaaaa aatgcacgag tggctaagtg
      601 tgatggaagt gaattttttc tagaactgcc aagcatgaat gcagactttc ccgcagggaa
      661 aatcatcctc aagctaggag attctggttt ttacaacaaa aggacaaaaa gtctcgaggg
      721 agcctatggc cttaggcata tatgggacaa gcatcgtgtc gagataggag ctacttccgc
      781 tgaggatatt gtcatctttt tggagagcat attattagct ggggcagaag tgctcataga
      841 tcctaaaaaa ggccaaaaca aagcgattgt tgtagagtca ggaacgggga tgatgatact
      901 agaattgaaa aaaccaaacg gcgaagaccc gtactacagc atcattactg cttatgatag
      961 gaagagccat ccaggtacaa aacttcatac cctaatatga aaaaagccgg ctaaatagcc
     1021 gacttcttcg taatagtgtt gttcgtggca ttcccttagc atttcggttc gggacaggtc
     1081 tttgacctta acaacgcatt gtcgaaatct ggtttccgac aagtagccta caaatatgta
     1141 accaatccag agttcaggtt acataccaag cccttctaca catacagaat acaactctat
     1201 cactgctcat ctttttgcaa aaagtgctat ttttgtgttc agtggaaacc aaaacgaagt
     1261 caaatcactg gtaaacggtc agatctcctt gtgatcgttt tactctcgtc gtaatctctc
     1321 tccctgtaaa cgaaaaaacc acccggcaag gtggtttttc gaaggttcag taaaccggca
     1381 agtttctgaa ccgtggtaac agtcttgtgc gagacatgtc accaaatact gtccttccag
     1441 tgtagcctca ctaaggcctc cacttcaaga actctcgata catctctcgc acatcctgct
     1501 taccagtggc cgctgccagt ggcgttaagt cgtgtctttt cgggttggac tcaagacgat
     1561 agttaccgga taaggcgcag cggtcggact gaacgggggg ttcgtgcata cagtccagct
     1621 tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagataa acgcggccat
     1681 aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagcca
     1741 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca ctgatttgag
     1801 cgtcagattt cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg caacgccgga
     1861 tccttttccc cttccttctt gatttgagcg ctattcatct cgtcattctt gagcaggtaa
     1921 cgcccgccgc agtctcacgg caggagcgta gcgaccgagt gagcgaggag gcgtcatttc
     1981 atcgtctcca gcatttcgca cttacgcccc acagctgaat tttttacctg ccacatgaag
     2041 cacttccctg aattcccact tcgatttacc atactaagcc agtatacact ccgctagcgc
     2101 tacgtgactg gttcagggct gcgccccgaa acccgctaaa accactgacg cgcctgcggc
     2161 ttgtccaaca ccgcgccgac cgggaaaaga tttcccgccc gtcccggcgt tatcaggagg
     2221 ccggattggc agacaaacgc agcaaaatgc tcacgatgtg ggtgaccgaa gatgagcacc
     2281 gccgtctgct ggagcgctgc gacggtaaac agcttgcagc ctggatgcgc cagacgtgcc
     2341 tggacgagaa acccgcccgc gccggcaagc ttccctcgct ctcgccggcg ctgcttcgtc
     2401 agcttgccgg catggggaac aacctcaacc agattgcccg gcaggttaac gccggtggtg
     2461 gcagcgggca cgaccgcgtg caggtcgtcg ccgcgctgat ggccatagat gccggactcg
     2521 agcggctgcg gcatgccgtg ctggaaaagg gtgcagacga tgatcgttaa gtttcacccg
     2581 cgagggcgcg gcggtggcgc cggtccggtg gattatctgc tggggaaaga ccgacagcgc
     2641 gacggcgcca ccgttctgca ggggaagccg gaagaagtca gggagcttat cgacgcctcg
     2701 ccctacgcaa aaaagtacac atccggcgtc ctgtcctttg ccgaaaagga tttaccgccc
     2761 gggcagcgcg aaaagctgat ggcgagcttt gaacgggttc tgatgcccgg actcgataaa
     2821 gaccagtaca gcgtgctgtg ggttgagcac caggataagg ggcggctgga gctgaacttc
     2881 ctgatcccga acacggagct gctgaccggc aagcgtctcc agccgtatta cgaccgcgcc
     2941 gaccgtccgc gcattgatgc ctggcagacc atcgtgaacg gcaggctggg gctgcatgac
     3001 ccgaacgcgc ctgaaaaccg gcgggcgctg gtcacgccgt ccgcgctgcc ggaaacgaag
     3061 caggaagccg ctcaggcaat tacacggggt ttactggcct tagcctcgtc cggggagctg
     3121 aaaacccgcc aggacgtcac tgaggcgctg gaaagcgcag gttttgaggt ggtacgcacc
     3181 acaaaaagca gcatcagcat tgccgacccg gacggggggc gaaacatccg actgaaggga
     3241 gccatctatg aacagtcttt taacgctggc gaaggactta gagcagaaat cgaaagcgca
     3301 gcagcagagt accggcgaga tgctgaaagc cgcattcagc gagcacgaga agtctgtcag
     3361 agcggaactg agcgaaagcg agaagagaat cagcgccgcc atcctcgacc acgacaggaa
     3421 gctgtccgct gccatgagcc agcgcacgaa aggtatgctg cggatggtca gccagacgtg
     3481 gctgaccatc gtcttggtgt ctacactgct gacagcatcg ggcgcaagca ttctgtggtg
     3541 gcaggggcag cagatactcg acaattacac gaccatccgg gagcagaagc gcacgcaggc
     3601 catgctgtcc gagaggaaca gcggtgtgca gctctcgacc tgcggagagc agagacgccg
     3661 ctgcgtgagg gtgaacccgg aagcgggacg gttcggagag gattcgagct ggatgatact
     3721 ggcggggaaa tagcacatga cggagctgga aaaacagttg ctgagcgcat tagagcagct
     3781 acagcaggac tactcgaaaa ggctggacga gtgggagaac gccttcgcgg aatggcggac
     3841 gatgtctggt cttatgcaac gggagaacgc ggcgctgagc gagcgcgtca cgggcttgag
     3901 caggcaggtg cagagtttga gcgagcagct gcgccggttg tcacaaggct gaacgtgata
     3961 gaggcacatc gtgagcagga acgggcggcg cagcatcaga aagcgctgga gctggaacga
     4021 tctcagcgac agcgaacgta caacggtccg tcactttgaa tctgtgcgcc ggaaaagcat
     4081 gggcttgctg tttctggcgt acaggttcag ttctagaaca tgaggctgct atcttgttgt
     4141 tcacgcttca tcagatcttg atgcttattg ttatggctga gctatcaatc cctgttgcgt
     4201 tgctaatatc ctttttggct ctttcaattg cctcgttaag ggagagtttt tctgttttct
     4261 cttcagccag accaggaatc actgtattag gaaccgatgg ggatgaagga tggaatttct
     4321 tccattccag ttgttccggt gttaccccaa tacgtaaaaa agcttcctgt gcgctcaggt
     4381 aagcaaccgg atagcgctga ccatcggtta gcattttcca tgcttttggc gtgtgaattt
     4441 ctgcgtattc atcaaaggtt atgacgttcc tgtcgtcgta atcagggctt ttaaaacacc
     4501 cggtaatttt ggcgatcagg aatgctgtgc cgtgttccag gtcttttact gcccacgttt
     4561 cccttctgtt tcttactgct atgaggtaat cggcatcgtt gagcctgtct gttctggcat
     4621 gccagtagcc tgaccctccc tcagttctca ttatgtt
//
