LOCUS       NZ_CP117625             8605 bp    DNA     circular CON 25-MAR-2024
DEFINITION  Escherichia albertii strain BIA_24 plasmid pEA24_5, complete
            sequence.
ACCESSION   NZ_CP117625
VERSION     NZ_CP117625.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33094100
            Assembly: GCF_028622415.1
KEYWORDS    RefSeq.
SOURCE      Escherichia albertii
  ORGANISM  Escherichia albertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8605)
  AUTHORS   Leszczynska,K., Swiecicka,I., Daniluk,T., Lebensztejn,D.,
            Chmielewska,S., Leszczynska,D., Gawor,J. and Kliber,M.
  TITLE     Escherichia albertii as a potential enteropathogen in the light of
            epidemiological and genomic studies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8605)
  AUTHORS   Leszczynska,K., Swiecicka,I., Daniluk,T., Lebensztejn,D.,
            Chmielewska,S., Leszczynska,D., Gawor,J. and Kliber,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2023) Department of Microbiology, Medical
            University of Bialystok, 2C Mickiewicza Street, Bialystok, Podlasie
            15-222, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. v1.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 196x
            Sequencing Technology :: Illumina Miseq/Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028622415.1-RS_2024_03_25
            Annotation Date                   :: 03/25/2024 01:31:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,144
            CDSs (total)                      :: 5,013
            Genes (coding)                    :: 4,772
            CDSs (with protein)               :: 4,772
            Genes (RNA)                       :: 131
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 241
            CDSs (without protein)            :: 241
            Pseudo Genes (ambiguous residues) :: 0 of 241
            Pseudo Genes (frameshifted)       :: 76 of 241
            Pseudo Genes (incomplete)         :: 184 of 241
            Pseudo Genes (internal stop)      :: 43 of 241
            Pseudo Genes (multiple problems)  :: 51 of 241
            Pseudo Genes (short protein)      :: 3 of 241
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117625.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8605
                     /organism="Escherichia albertii"
                     /mol_type="genomic DNA"
                     /strain="BIA_24"
                     /host="Grus grus"
                     /db_xref="taxon:208962"
                     /plasmid="pEA24_5"
                     /geo_loc_name="Poland: North-East"
                     /collection_date="2015"
     gene            join(7095..8605,1..1450)
                     /locus_tag="PS039_RS25610"
                     /old_locus_tag="PS039_25610"
     CDS             join(7095..8605,1..1450)
                     /locus_tag="PS039_RS25610"
                     /old_locus_tag="PS039_25610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_273774644.1"
                     /translation="MSTNEKFLTQRQRYQPVMLPQDFSDEEMARDWTLTTSDLLEIERY
                     RARFRLFISLQICAVRLYGRFIQDIGSVSPRIVSYLNNQLSLPPSLTVASPEREATLAE
                     QRKNILTYLGFTRYDENIQSNLKLWLVKQAEKGFLPDALFLRAEQYLLSSRVILPGPTI
                     IERLIISTCAQVHERMFDTLYKQLSAGIKQTIDDLLVTLPGDQRSLFYLLKESPPSATA
                     ISIKRYMKRYYVIDNCELDAVNSVIVDPAFMTYLYKLAYRYSAKDIKRFKAPKRYSLML
                     CFLLETRKILLDNLIKMHDQFIMELLRHGKRLHDQKHRELRKRQKKAIDTILQVTNWLL
                     GSQDDRPLFKKELWQSVSETKLLESVNDLNAFKRLEERGLGDILVTRYPGFRKYFSEFL
                     RLPFKAKPGTEQLLNAIMLLRQLDDGEIKRLPVNVPTHFVPHELQRILKGKDGKLQRNA
                     WELGVAIAMKDALRSGDLFVPQSKQYVSFWDLMLDPHHWQATREGVYSALQQPTQEYVK
                     TALILEFHKIVGETEKQFEFDSFAEIKDGKLRLQRDDKAEIPEAVTRLQKVIDASMPSI
                     RIEQLLMEVDQQTHFSRHFIPVQQHNSRPKHFYKTLIAALISQATNLGVVAMSTSVNDV
                     SVDMLRHVLQFYIREDTIKSASAEIVNQHHQLPFSQIHGSGQLSSSDAQRFGIRADSLL
                     AAYYPRYYGYYEKAIGIYTHVSDQYSVYSTKVISCNPREALYVLDGILENNTILKIREH
                     TTDTHGYTEIIFALCYLLGYYFMPRIKDLKDQQLYRVDRTVNYGVFTPLLNKTIDLDII
                     EEQWDAMIHVSQSLKERTAPAHVIVERLTNSFPSDRLSKAFTNLGRIIKTEYILRYITD
                     KKLRRTVQLQLNKGEYRHKLPRWIFFANQGEFTTGDYEEIMNKASALSLVSNAILYWNT
                     SRISNIIEELRKNGEIIDDDILAHISLLPYKHVLPNGTYFVDGEPYGGKSTEGAKP"
                     /name="Tn3 family transposase"
     gene            1505..1690
                     /locus_tag="PS039_RS25615"
                     /old_locus_tag="PS039_25615"
     CDS             1505..1690
                     /locus_tag="PS039_RS25615"
                     /old_locus_tag="PS039_25615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005421809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094307661.1"
                     /translation="MSKKDAKILLRSFFSARNLLLANKKTTATNGGLFAGSRATNSFSE
                     GNWLGGAQSPKPVFSV"
                     /name="hypothetical protein"
     gene            complement(1730..1843)
                     /locus_tag="PS039_RS25620"
                     /old_locus_tag="PS039_25620"
     CDS             complement(1730..1843)
                     /locus_tag="PS039_RS25620"
                     /old_locus_tag="PS039_25620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071821494.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(2512..2703)
                     /locus_tag="PS039_RS25625"
                     /old_locus_tag="PS039_25625"
     CDS             complement(2512..2703)
                     /locus_tag="PS039_RS25625"
                     /old_locus_tag="PS039_25625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3534..3953)
                     /locus_tag="PS039_RS25635"
                     /old_locus_tag="PS039_25635"
     CDS             complement(3534..3953)
                     /locus_tag="PS039_RS25635"
                     /old_locus_tag="PS039_25635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_273810689.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISSVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(4034..4471)
                     /locus_tag="PS039_RS25640"
                     /old_locus_tag="PS039_25640"
     CDS             complement(4034..4471)
                     /locus_tag="PS039_RS25640"
                     /old_locus_tag="PS039_25640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_273810690.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTSQDGRR
                     EFETSELIRAYGELKRNETPERHSAGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAEREQLQNEIAQLRQALEQEKKRGFWSRLFAR"
                     /name="entry exclusion protein 1"
     gene            4732..5493
                     /locus_tag="PS039_RS25645"
                     /old_locus_tag="PS039_25645"
     CDS             4732..5493
                     /locus_tag="PS039_RS25645"
                     /old_locus_tag="PS039_25645"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_273810686.1"
                     /translation="MPGFNYGGHGDGTGWSSERGDGPAPGGGSHGNSGRDDHRGESGYG
                     YPGHNGISGPVSLRTTDSYDGDTMSDYINNLINHEGFKNTMYRDTEGNITVGIGHLLSS
                     PEMAAGLPFNRTRITHGHGDEMEHEFSVSREDITSSFNALRDNPTAISGIHLSNDAVIG
                     LAISDVESTISGLKGLYSDFDSFPRSAKTALVDMGFNVGVGKLRSDFPNFNNAVNKKDW
                     NTAADESHRTKIGEGRNNDTKAQFQQAANGN"
                     /name="hypothetical protein"
     gene            complement(5560..6186)
                     /locus_tag="PS039_RS25650"
                     /old_locus_tag="PS039_25650"
     CDS             complement(5560..6186)
                     /locus_tag="PS039_RS25650"
                     /old_locus_tag="PS039_25650"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_273810687.1"
                     /translation="MRHIFFLLYFFCISTIASNELKINNMPIELIFNDGSSAKISSCTD
                     LQNQMSLGKKIESIPNISDPDYNDIKNKIFICRMQNYISNHRMTRIKDSLSIHDVLNNF
                     PATAAYFISQEEMDKVSREYSNKTILEYTPDLIIDKNKAISQKKYTSYSLPEYYHFKNS
                     DGDVIKIVILAGHSIGGTASSRSYWRIDDDHSHPWTVTELDENSF"
                     /name="hypothetical protein"
     gene            complement(6315..6875)
                     /locus_tag="PS039_RS25655"
                     /old_locus_tag="PS039_25655"
     CDS             complement(6315..6875)
                     /locus_tag="PS039_RS25655"
                     /old_locus_tag="PS039_25655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012192705.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_021573890.1"
                     /translation="MLVGYARVSTDDQNLNLQRDALNQAGCEQIFEDQLSGAKAERPGL
                     YSALQYARAGDTVVVWRLDRLSRSLKDLIEMVSLLESRGIGLRSLQEAIDTSSSSGKLI
                     FHIFGALAEFERNLIRERTQAGLKSARARGKKGGRPKSLSKDKQALAVRLYDERKHTVT
                     QICEIMGISKPTLYKYIAAARAG"
                     /name="recombinase family protein"
     Typing          2340..2424
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.674"
                     /coverage="100.0"
                     /evalue="6.569999999999999e-34"
     Typing          complement(1772..2314)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.333"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ttatagtgcg ctgcaacaac caactcagga atacgttaag acagcactaa ttcttgaatt
       61 ccataagata gttggtgaaa cagaaaaaca gttcgaattt gattcttttg ctgaaattaa
      121 agacggaaaa ctgcgacttc aacgggacga taaagctgaa attccagaag cagtcactcg
      181 actacaaaag gtcattgatg ccagtatgcc ctctattcgc atagagcaac tactaatgga
      241 agtcgaccaa caaactcatt tcagcaggca tttcatacct gttcagcagc ataacagcag
      301 gccaaaacat ttttataaga ctctcattgc tgcactgata tcacaagcaa ccaatcttgg
      361 cgtggttgct atgagtacca gcgttaatga tgtcagcgtc gatatgttac gacatgtatt
      421 gcaattttat attcgggaag acactattaa atccgccagt gctgaaattg taaatcagca
      481 tcatcaactt ccttttagtc aaatacacgg ttcagggcaa ttatcgtctt ctgatgcaca
      541 acgtttcggc atcagggcag atagtctcct ggctgcatat tatccacgct attatggtta
      601 ttacgaaaaa gcgatcggga tatatacgca cgtatcagat caatattcgg tatatagtac
      661 caaagtaatt tcctgcaatc ccagagaagc cttatatgta cttgatggaa ttttagaaaa
      721 taataccata ctgaaaattc gggaacatac cacagacacg cacggctata ccgagataat
      781 tttcgcactt tgttatcttc ttggctatta ctttatgcca cgaatcaaag atctaaagga
      841 tcaacaactt tatcgggttg accgtacagt taattacgga gtttttacac cattactgaa
      901 taaaacaata gatctggata ttattgaaga gcaatgggat gcgatgattc atgtatcaca
      961 atctctgaaa gaacgtactg caccagctca tgtcatcgta gagaggctca ctaatagttt
     1021 tccatctgat cgtctttcta aggcatttac aaatcttggc agaattatta agacagaata
     1081 tattctacgc tatataactg ataaaaaatt acgacgtacg gttcaacttc agcttaataa
     1141 aggtgagtat cgtcataaat taccaagatg gattttcttt gcaaatcagg gggagttcac
     1201 gactggcgac tacgaagaaa tcatgaataa agccagtgcg ttgagtctgg tatcaaatgc
     1261 aatcctttac tggaacactt cgagaataag taatattatt gaagaattac gtaaaaatgg
     1321 tgagattatt gatgatgata tattagctca tatttctttg cttccctata agcacgtatt
     1381 acccaacggt acttattttg ttgatggaga gccttacggt ggtaagtcaa ccgaaggggc
     1441 aaaaccgtaa gtttctgttc aaatgccccc gctggtcctg aatgtattta aacaggaaag
     1501 tattttgagt aaaaaagatg ccaagatcct cttgagatcc tttttttctg cgcgtaatct
     1561 gctgcttgca aacaaaaaaa ccaccgctac caacggtggt ttgtttgccg gatcaagagc
     1621 taccaactct ttttccgaag gtaactggct tggaggagcg cagtcaccaa aacctgtctt
     1681 ttcagtttag ccttaactgg ttcataactt caagaccaac tcctctaaat cagttaccag
     1741 tggctgctgc cagtggcgtt aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1801 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1861 gaacgaccta caccaagccg agataccaac agcgtgagct atgagaaagc gccacgcttc
     1921 ccgaagggag aaaggcggac aggtatccgg taagtggcag ggtcggaaca ggagagcgca
     1981 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     2041 tctgacttga gcgtcgattt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     2101 ccgtggcgcg gccttttgtt tccctttgcc tttttgctca catgttcttt ccggccttat
     2161 cccctgattc tgtggataac cgcattatcg tttttgagtg agcagacacc gctcgccgca
     2221 gccgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaagagcgt cggaagttgc
     2281 cttttctcct tacgcatctg tgcggcattt cacatcggct atggtgcact tttcatacaa
     2341 tccgcaccga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt
     2401 gatggctgcg ccccgacacc cgctaaaacc cgctgacgcg ccctgacggg cttgtctgct
     2461 cccggcatcc gctcacagac aagctgtgag cgtctccggg agctgcatgt gtcagaggtt
     2521 ttcaccgtca tccccgaaac gtgcgaggca actgcggtaa agctcatcgg cgtggtcgtg
     2581 aagcgattca cagatatctg cctgttcatc cgcgtccagt tcgttgagct tctccagcag
     2641 ggttaatgtc tggcttctga tgaagcgggc catgttcagg gcggtttttt cctgtttggt
     2701 cactggtctg atgtctccgt gtaagggaat ttctgttcat gggagcatga taactgtgac
     2761 gcaggcgaga gtgataaagc tgagtgagcg gttagtcagg ggcgcaaaca ttcgaacgcg
     2821 cccagtgcga tgcaggatag ggaaaacgcc gtaaaacgac gtccctttcg gcaatagcaa
     2881 tatcatggca cagcatatca actgataaat aatccctgtg cagtacagac gctgttgcat
     2941 aaaatcgcgc tctgcggcga gtgtttttta atcagatatt ccgctgatac tccggaaaaa
     3001 cgcgagtctg ttctcatgtc tgaaatgtcc acaccccacg caaaaccaac tttttgctga
     3061 tttgcttttt aaatcatgtt gttatggttt taaaaatgta ttttcttatt cagatttata
     3121 caaaagcacg tcgtcgacga ccctatggcg acgctatgac gaccctactt agggtcagta
     3181 ctgactatct gtagaaaaaa cagttaatta atgtaggggc gcccctaggg gcgcgtcgcc
     3241 tttttttaca taacacccct gggggcgctg ccaggggtgt ctgcccggat atgtagcaat
     3301 gagccaggga ttcacgaact gaaaatgtcc gaaaatgtgc ctcatgggca ccgtatgtaa
     3361 ccaaaccgat cgccattcac tttcatgcat agctatgcag cgagctgaaa acgatcctga
     3421 cgcatttttc cggtttgtac cgggtaaaac atctcttttt gaggggtttg cgccagaatc
     3481 gcgttcagcg cgtttcagcg gtgcgtacaa ttaagggatt atggtaaatc cgcttactgt
     3541 cttccctcgt agccatcgag ataaaccgaa gaaatcgtct cagccagcag cctggatttt
     3601 ttcggggtaa ggtctttgct gcggtaatca gtacccgcaa attgagttgt gccccggagt
     3661 gagtttaaaa attcctggct gtgaaactgc tgaatgcgtt gctgcacatc actccgtgat
     3721 tttcctgtcg ctttatcttt agctcccata tcccagaact gccgaaaaaa aggcaccatc
     3781 attcgtgttg tgtcggcttt ttgggtggaa aagttgggat caaaaccgtc atcagaagca
     3841 tatacgtaat gccgggtatg ttgttccacg ctgccctttg gagtacaggc tgtgagagct
     3901 actgttgctg ttatcatggc tataagacaa agtttgtttc cggtacgtct catgagtgtc
     3961 tccctacctt aaagtattta ttttcggtga gttattatcc gaagactatt ttatcatcag
     4021 tctctgacag cgttcagcga gcaaacaacc gggaccagaa tccccgtttc ttttcctgct
     4081 ccagcgcctg cctgagttgg gctatctcat tctgtagctg ttcccgttct gcttcctggc
     4141 gccggcgctc cgtatcctgg gcttgtttat cctcaagcat cagcgtcagg cattgtttca
     4201 gctcattcag ctcccggaga atgcgttctg tctgctgatc atgtggattt tctgcctgtc
     4261 ccgcactgtg cctttctggt gtctcattcc gctttaattc gccgtatgcc cggatcagtt
     4321 cactggtttc aaactcccgt cgtccgtcct ggcttgtacg gtaactgaca aggcctgcgc
     4381 gcatatcacg gtaaaactgt gaacgcgatc tgcccgtcat tttcagtgcg tcccggagtg
     4441 tgtgccatgc cataaagtga cagtgtccca taggtgtctc atctcatggt ctcagtaaaa
     4501 catactgagg ccggagaacg gtaatgtttg tgctggtttt tgtggcatct ggcggggatg
     4561 gcacatggtg tgagtggctg tttttttgac cgttttcaca aaaatgaaaa tccacagggt
     4621 tgacagggaa aacgcagcgg cgtaattttt aatctgtata taaaaccagt ggtcatatat
     4681 acagtatttt gtttttaacg gtttgtttta caagtcaaag aggagttagt tatgcctgga
     4741 tttaattatg gtggacatgg tgacggtact ggctggagtt ctgaacgtgg tgatggtccg
     4801 gctccaggag gcggttctca tgggaatagt ggtcgagacg atcatcgtgg tgaaagtggt
     4861 tatggttatc caggtcacaa tgggatatct gggcctgtga gtctcaggac aacagatagt
     4921 tatgatggtg atactatgtc agattatatt aacaacttaa tcaatcatga aggttttaaa
     4981 aatacaatgt atagggatac cgaagggaac ataactgtcg gtattggtca cttgctttca
     5041 tcaccggaga tggcggctgg attaccgttt aaccgaacta gaattacaca tggacatggt
     5101 gatgaaatgg aacatgagtt ttctgtatcg cgggaagata ttacatcttc atttaatgca
     5161 ttgagagata atcctacagc tatttctggt attcaccttt caaacgatgc agtaattggt
     5221 cttgctatta gcgatgtaga atcgacaatt tcaggattaa aagggcttta ttctgatttt
     5281 gatagtttcc ctcgttcagc aaaaaccgca ttagttgata tggggttcaa tgtgggagtt
     5341 ggtaaattac gatcggactt tccaaacttt aataatgctg taaataagaa agattggaat
     5401 actgcggctg atgagtctca tcgaactaag ataggtgaag gccgaaataa tgatacgaaa
     5461 gctcagttcc aacaagcagc taatggtaac taagcataaa ggattctaca tatttcatgc
     5521 ctataccacc tctttatgag gtggtaatta attgaattat cagaaactat tttcatcaag
     5581 ttcagtgact gtccaggggt gtgaatgatc atcatctata cgccagtatg agcgtgagct
     5641 agctgtacca ccaattgaat gccctgctaa aataacaatt tttattacat caccatcact
     5701 atttttaaag tgataatatt caggtagtga gtagcttgtg tatttttttt gtgatattgc
     5761 tttattttta tcaataataa gatccggtgt gtattctaaa attgtcttgt tgctgtattc
     5821 acgagaaact ttatccattt cttcttgact tataaaatat gccgctgttg ctggaaaatt
     5881 attcaaaacg tcatgtattg ataatgagtc tttgattctc gtcattcgat gatttgatat
     5941 gtaattttgc atcctgcata taaatatttt attttttata tcattgtaat ctgggtctga
     6001 aatgttaggt attgactcta ttttttttcc tagtgacatc tgattctgaa gatcagtgca
     6061 agatgatatt tttgcactgc tcccgtcatt aaaaattaac tctataggca tgttgttaat
     6121 ttttaattca tttgaagcta ttgttgatat gcagaaaaaa taaagcaaaa agaatatgtg
     6181 acgcatactt agggttctcc tacattagtt gttaatttat gttgtactga atggaccagc
     6241 gggggcattt gaacagaaac ttacggtttc aactgctgaa caaaggatga aaccgtaatt
     6301 tcttgaatgt gagtttatcc ggctctggct gctgcaatat atttatacag ggtaggtttt
     6361 gaaattccca ttatttcgca gatctgtgta accgtgtgct tcctttcatc gtacaacctt
     6421 acagccaggg cttgtttgtc cttactaaga gacttgggtc tccctccttt tttaccacgt
     6481 gcccgtgcag atttgagtcc tgcctgggtt cgttcacgga tcaggttacg ttcaaactct
     6541 gccagtgcac caaatatatg aaaaattaac ttacctgaac ttgatgatgt atctatagct
     6601 tcctgaagac ttctcagacc aatacctctg gactccagta acgacaccat ttctatcaga
     6661 tccttaagtg aacggctcag gcgatccagt cgccagacca caacagtgtc acccgctctt
     6721 gcatactgta aagcactata caagccaggg cgttcagctt tagcaccact aagttggtct
     6781 tcgaatattt gctcacatcc agcctggttg agggcatctc gttgaagatt gagattctgg
     6841 tcgtcggtag aaactctggc atacccaacc agcatatatg tatccctcag aagtaaaaaa
     6901 acttgtcaca ttaccaggta tattaacata gatatattaa ccgagtatat taaccacgaa
     6961 acgattgtaa atctgttgtt ttgtcggagg cctgaaaggc agtaaacaaa acgagcgttt
     7021 tctttacatc ctgtgcgtaa cgatgttcgc cataaacata tcaacgggac ggtaaacttc
     7081 agaaaggggt aacgatgtca acaaacgaaa aatttctgac tcagcgacaa cgctaccagc
     7141 cagttatgtt gccacaggat ttttctgatg aggaaatggc acgggactgg acattaacta
     7201 catctgatct actggagatt gagcgctatc gtgcccgttt ccgcttattt atctcccttc
     7261 agatttgcgc agtccgtctt tatggacgat ttattcagga cattggtagc gtatcaccac
     7321 gcattgttag ttatctgaat aaccaacttt ctttaccacc atcactgaca gttgcgtcac
     7381 ctgaacgaga agcgacactg gctgaacagc gtaaaaatat actgacttat cttggtttta
     7441 cccgttatga cgaaaatatt cagtcaaatc ttaaactttg gttagtaaaa caagctgaga
     7501 aaggtttctt acctgatgct ctgtttctcc gggctgaaca atatttactt tcttctcggg
     7561 taattttacc aggaccaaca atcattgagc gtctaattat cagcacttgt gctcaggtcc
     7621 atgaacgaat gttcgacact ctgtataaac agctttctgc cgggattaaa cagactattg
     7681 atgatctatt agtaacctta cctggcgatc aacggtcatt gttctatttg ctcaaggaat
     7741 caccaccatc agccacagct atttcaatta agcgctatat gaaacggtat tatgtgattg
     7801 ataattgtga gcttgatgct gtcaattctg ttattgttga tcccgcattt atgacttacc
     7861 tatataaact ggcatatcgt tactctgcca aagatattaa gcgtttcaaa gcaccaaaac
     7921 gttattcttt aatgctatgt tttttattag aaacacgtaa aatattgctc gacaatctga
     7981 taaaaatgca cgaccagttt ataatggaat tattgcgcca tggaaaacgc cttcatgatc
     8041 aaaagcatcg tgagttacgc aagcggcaga aaaaggctat agatacaata cttcaggtaa
     8101 caaactggct cctggggagt caggatgaca gaccattatt taaaaaagaa ctctggcaat
     8161 ctgttagcga aacaaaacta ctggaatcag tgaatgatct gaatgctttt aaacgccttg
     8221 aggaacgggg acttggtgat attcttgtta cccgttaccc aggattccgt aaatactttt
     8281 cagaatttct tcggttacct tttaaagcaa aaccaggaac agaacaactc ctcaatgcaa
     8341 ttatgctcct gcgtcaactt gatgatggcg aaataaaaag attaccagtc aatgttccta
     8401 cccattttgt accacatgag ttacagcgaa tattgaaagg gaaggatgga aaattacaac
     8461 gaaacgcatg ggaacttgga gtagctattg ccatgaagga cgcccttcgt tcaggcgatc
     8521 tctttgtacc tcagagcaaa cagtacgttt cgttctggga tctaatgctg gatccacatc
     8581 actggcaggc aacccgggaa ggggt
//
