LOCUS       NZ_CP117608             4593 bp    DNA     circular CON 25-MAR-2024
DEFINITION  Escherichia albertii strain BIA_29 plasmid pEA29_2, complete
            sequence.
ACCESSION   NZ_CP117608
VERSION     NZ_CP117608.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33094103
            Assembly: GCF_028622255.1
KEYWORDS    RefSeq.
SOURCE      Escherichia albertii
  ORGANISM  Escherichia albertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Leszczynska,K., Swiecicka,I., Daniluk,T., Lebensztejn,D.,
            Chmielewska,S., Leszczynska,D., Gawor,J. and Kliber,M.
  TITLE     Escherichia albertii as a potential enteropathogen in the light of
            epidemiological and genomic studies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4593)
  AUTHORS   Leszczynska,K., Swiecicka,I., Daniluk,T., Lebensztejn,D.,
            Chmielewska,S., Leszczynska,D., Gawor,J. and Kliber,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2023) Department of Microbiology, Medical
            University of Bialystok, 2C Mickiewicza Street, Bialystok, Podlasie
            15-222, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. v1.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 285x
            Sequencing Technology :: Illumina Miseq/Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028622255.1-RS_2024_03_25
            Annotation Date                   :: 03/25/2024 01:26:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,683
            CDSs (total)                      :: 4,563
            Genes (coding)                    :: 4,369
            CDSs (with protein)               :: 4,369
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 194
            CDSs (without protein)            :: 194
            Pseudo Genes (ambiguous residues) :: 0 of 194
            Pseudo Genes (frameshifted)       :: 73 of 194
            Pseudo Genes (incomplete)         :: 127 of 194
            Pseudo Genes (internal stop)      :: 54 of 194
            Pseudo Genes (multiple problems)  :: 50 of 194
            Pseudo Genes (short protein)      :: 3 of 194
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117608.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Escherichia albertii"
                     /mol_type="genomic DNA"
                     /strain="BIA_29"
                     /isolation_source="bird (unidentified species)"
                     /db_xref="taxon:208962"
                     /plasmid="pEA29_2"
                     /geo_loc_name="Poland: North-East"
                     /collection_date="2016"
     gene            join(4311..4593,1..41)
                     /locus_tag="PS031_RS23355"
                     /old_locus_tag="PS031_23355"
     CDS             join(4311..4593,1..41)
                     /locus_tag="PS031_RS23355"
                     /old_locus_tag="PS031_23355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001677263.1"
                     /translation="MLTIRVSDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGHWSSIDRVHVVAALMAIEGELRQLRQAVREQGGR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            250..>684
                     /locus_tag="PS031_RS23360"
                     /old_locus_tag="PS031_23360"
                     /pseudo=""
     CDS             250..>684
                     /locus_tag="PS031_RS23360"
                     /old_locus_tag="PS031_23360"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            737..1279
                     /locus_tag="PS031_RS23365"
                     /old_locus_tag="PS031_23365"
     CDS             737..1279
                     /locus_tag="PS031_RS23365"
                     /old_locus_tag="PS031_23365"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_230905694.1"
                     /translation="MGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNRQKIRDA
                     ISEHTTSVKQQLDTLSTTVSTQLSTTEAELSRQQKNLLWQVIKGRVLFPALTALSVTGG
                     IFLGCWGLIQWQESRIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVLPDGVKG
                     ENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            1286..1519
                     /locus_tag="PS031_RS23370"
                     /old_locus_tag="PS031_23370"
     CDS             1286..1519
                     /locus_tag="PS031_RS23370"
                     /old_locus_tag="PS031_23370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137388.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHAVTQQNNA
                     ILNERVVNLSQQVQLLAGQVDRLSRLFITNNR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2207..3046)
                     /locus_tag="PS031_RS23375"
                     /old_locus_tag="PS031_23375"
     CDS             complement(2207..3046)
                     /locus_tag="PS031_RS23375"
                     /old_locus_tag="PS031_23375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001354646.1"
                     /translation="MLTLFIFFVLLIAACFFCFAPPRRGYDRNEIIPYKIKLSINKYRL
                     YIYSSGKVRQYLLFLVILSLYYSIAEPFKSELIKNISYSLMAAFIFDTGLNFSKENITK
                     GVISTRWHNDLYSSFERMKAINKIYYPSNKEINTEGLSKAITSSLFNDDANSFAKRDFR
                     LMWDLSSEKYLSYKEIIIRKGDKLDAVCLRFINDDYKFLVNFNRDEEVFKYFPSIMQPS
                     LKTYRALSRLVNSIKDPSRFKFTTESLEMELLEYLELRNELFNDIEEVMGSYAQRAP"
                     /name="hypothetical protein"
     gene            complement(3238..3387)
                     /locus_tag="PS031_RS23445"
     CDS             complement(3238..3387)
                     /locus_tag="PS031_RS23445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032194431.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPERHVKTPLAAAIGNRLEELVLK
                     LCAG"
                     /name="hypothetical protein"
     gene            complement(4057..4248)
                     /locus_tag="PS031_RS23380"
                     /old_locus_tag="PS031_23380"
     CDS             complement(4057..4248)
                     /locus_tag="PS031_RS23380"
                     /old_locus_tag="PS031_23380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165986.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          3885..3969
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          complement(3316..3874)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.761"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          32..1617
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4279..4592
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="82.857"
                     /coverage="89.0"
                     /evalue="6.55e-51"
ORIGIN
        1 ggcaggctgt cagggaacag gggggacggg atgatagtta aatttcatgc cagggggaaa
       61 ggcggtggaa gtggcccggt cgattacctg ctggggcggg agcgtaaccg cgaaggcgca
      121 acggtgctcc ggggtaaccc ggaagaaatc cgggaactca ttgatgccac gccattttcg
      181 aagaaataca cgtcaggcgt tctgtcgttc gcggagaagg agttaccgcc gggtgaacgc
      241 gaaagagtga tgacgagctt tgagcgtgtt cttatgcccg gtctcgaaaa agaccagtac
      301 agcattctct gggtggaaca ccaggataag gggcggctcg aactgaattt tgtcattccg
      361 aacatggaac tacagagcgg taagcgcctc cagccgtact acgaccgtgc ggacaggccg
      421 aggataaatg cctggcagac gctggttaat catcattacg ggctgcgtga cccgaacgca
      481 ccggagaaca gacggacgct tgtcacaccg aataatctgc caaaagcgaa acaggaggcc
      541 gcagaagcga ttacacgggg tcttgaggcg ctttaccgtg ccggagcaat aaaaacgcgt
      601 caggatgtca ctgaggcgct cacagcggca ggttttgagg tggtgaggac aaccagaagc
      661 agtatcagta ttgccgaccc ggaggggggg cggaatctca ggctaaaggg ggcgctgtat
      721 gagcaatctt ttacaaatgg gaacggactt cgagaagaaa ctgaaagagc gagccgcttc
      781 taccgagaac atgctgaaca gcgagttcag gaagctagaa gagtctgtag acaaggcact
      841 gagcttaaac aggcagaaaa tcagagacgc tatcagcgaa catacaacat ccgtgaaaca
      901 gcaactggat acgctgagca ccaccgtgag cacgcagctc agcaccacag aggcagaact
      961 gtcccggcag cagaaaaacc tgctctggca ggtgatcaaa gggcgggtac tgtttccggc
     1021 gctgaccgcg ctcagcgtca cgggcggcat tttcctgggg tgctgggggc tgattcagtg
     1081 gcaggaaagc aggattgcga aaaatatctt gaccatcaga gagcaggaaa acacgctggc
     1141 gaaactcgag gcaaaaacct ggggggtgac gttcgtcaat ggcgagaacg ggaaatttct
     1201 ggtgctgccg gacggggtga aaggggaaaa cacctggacg gtgggcgaca agaacgccgt
     1261 gaggctggtc agggagtaaa cgagtatgac ggaactggaa gggcatttgc tgaacgcctt
     1321 agagcatttg caacaggact atatgcggcg gctgaacgag tgggagagcg ccttcgcaga
     1381 attgcagaag atgcacgcgg ttacgcaaca gaacaacgcg atactgaacg agcgtgtcgt
     1441 gaacttgagt cagcaggtgc agctattagc cggacaagtc gaccgcttga gtcggttatt
     1501 tatcacgaac aacagataag ggatgaacag gagcgtcagg cgcaggagct tgcccggcgc
     1561 tggcatgaag agcaggaacc cgaagcgcgg atctggcgtg gcccgtcaat ggggatgtga
     1621 ccggggtacc ccctgaaatc gagccagttt gtggcacgcc ctacccggat ggcgccaatg
     1681 ttaatgtacg tacattagca ttaacaatat ccaaacctcc ccattttgcc ccgttcgacg
     1741 gctggaacgt gcgaaactga tcgtcattca gtttcgcgca taactatgca tgaggttaaa
     1801 atttaccagg cgcgatcgcg gcagtttttc gggtggtttg ttgctgtttt tacctgtctg
     1861 ctgccgtgat cgcgctgaac gcgtttaagc ggtacgcgca atgcgacgtt atggtaaata
     1921 cggagtattt taattgcggg cctttgttgt atatctctgt cgtttgtccc cggggggaag
     1981 cattactgct aacaagtccc gtgagaaaac tgcgcaatcg tattgcgcac catgaaccta
     2041 ttctcaaccg caatctggag gatgattttg ctacgataaa gagaattatc gcgtatcggt
     2101 gtcagcattc attggagtgg atgctgaaaa accaggtgtt actccctcta cttacactta
     2161 aacctctcta aaaatataag taagtactat cgagggctat catgaactat ggtgccctct
     2221 gagcatagct acccattact tcttctatgt cattgaataa ttcatttctt aactctaagt
     2281 attctagtaa ctccatttct aatgactctg tcgtgaactt aaatctagaa ggatctttga
     2341 ttgagtttac tagtcttgat aacgctctat atgttttgag tgaaggttgc attatagatg
     2401 gaaagtattt gaaaacctct tcatctctgt tgaaattaac taaaaacttg tagtcatcat
     2461 ttataaatct caagcataca gcgtcaagct tatcgccttt tcttattatt atttctttat
     2521 aggaaagata tttttcagaa gataagtccc acatcaagcg aaaatctctc tttgcgaacg
     2581 agttcgcgtc atcattgaaa agagatgatg tgatggcttt ggatagacct tctgtgttta
     2641 tctctttgtt ggatgggtag taaattttat taattgcttt cattctctca aatgatgaat
     2701 ataggtcatt atgccacctt gttgaaatca ctcctttagt tatattctcc ttgctaaaat
     2761 taagccctgt atcaaaaata aatgcagcca tcagtgaata tgatatgttt tttattaact
     2821 cagacttaaa tggttccgct atggagtagt acagtgataa aataactaaa aataaaaggt
     2881 attgtcttac ctttcccgat gagtatatgt aaaggcgata tttatttatt gatagtttta
     2941 ttttgtatgg aatgatctca tttctgtcat atcctcttcg tggtggagca aaacaaaaga
     3001 aacatgcagc tattaataaa acaaaaaaga tgaacagtgt aagcaagttc ggctcctgtg
     3061 tatgctcact atgcatgatt gtcttgagat ctaacgatcc tgaaggtagt cttgtccgta
     3121 aacgaaaaaa ccgcccagca aggcggcttt tcgaaggttc tcagagcagc aactctttga
     3181 accaaggtaa ctggcttgga ggagcacagc caccaaatct gtcctttcag tttagcctta
     3241 accggcgcat aacttcaaga ctaactcctc taaacggtta ccaatggctg ctgccagtgg
     3301 cgtttttacg tgtctttccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3361 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccgg
     3421 gccgagatac caacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3481 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3541 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcatct
     3601 gtttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagcg gcgcggcctt
     3661 cttcctgttc ctggcctttt gctcacatgt ttcttttggc gttatcccct gattctgtgg
     3721 ataaccgcat caccgtcttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3781 gtagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggcatttt ctccttacgc
     3841 atctgtgcgg tatttcacac cgcatatggt gcacttttct tataatccgc actgatgcca
     3901 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcaggg ctgcgccccg
     3961 acacccgcta aaacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctca
     4021 caggcaagct gtgaacgtct ccgggagctg catgtttcag ttgctttcac cgtcatcgcc
     4081 gaagcgtgcg aggcaactac ggtagagttc gtcggcgtgg tcgtgaagtg attcgcagat
     4141 gtctgcctgt tcgtccgcgt ccagctcgtt gagtttttcc agcagggtca gcgtctggct
     4201 tctgataaag cgggccatgt taagggctgt tttttcctgt ttggtcatgg tcttaaggct
     4261 ccttaatagg ggatttttta gggggaatga taccgatgaa acgcgagaag atgctcacga
     4321 tacgggttag cgatgaggag cacgcccggt tactggaacg ctgcgagggt aaacgactgg
     4381 cagaatggat gcggcgggtc tgtctggggg agccggttgc gcgcacagga aaactgccga
     4441 cactatcgcc gccattgctg cggcatctgg ctgccatcgg gaacaatctg aaccagacag
     4501 cccggaaggt gaacagcggc cactggtctt cgattgaccg ggttcatgtg gtggccgctc
     4561 tgatggccat cgagggtgaa ttgcgccagt tgc
//
