LOCUS       NZ_CP117599             2571 bp    DNA     circular CON 25-MAR-2024
DEFINITION  Escherichia albertii strain BIA_35 plasmid pEA35_4, complete
            sequence.
ACCESSION   NZ_CP117599
VERSION     NZ_CP117599.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33094105
            Assembly: GCF_028622475.1
KEYWORDS    RefSeq.
SOURCE      Escherichia albertii
  ORGANISM  Escherichia albertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Leszczynska,K., Swiecicka,I., Daniluk,T., Lebensztejn,D.,
            Chmielewska,S., Leszczynska,D., Gawor,J. and Kliber,M.
  TITLE     Escherichia albertii as a potential enteropathogen in the light of
            epidemiological and genomic studies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Leszczynska,K., Swiecicka,I., Daniluk,T., Lebensztejn,D.,
            Chmielewska,S., Leszczynska,D., Gawor,J. and Kliber,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2023) Department of Microbiology, Medical
            University of Bialystok, 2C Mickiewicza Street, Bialystok, Podlasie
            15-222, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. v1.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 321x
            Sequencing Technology :: Illumina Miseq/Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028622475.1-RS_2024_03_25
            Annotation Date                   :: 03/25/2024 01:35:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,670
            CDSs (total)                      :: 4,558
            Genes (coding)                    :: 4,308
            CDSs (with protein)               :: 4,308
            Genes (RNA)                       :: 112
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 92 of 250
            Pseudo Genes (incomplete)         :: 144 of 250
            Pseudo Genes (internal stop)      :: 61 of 250
            Pseudo Genes (multiple problems)  :: 41 of 250
            Pseudo Genes (short protein)      :: 3 of 250
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117599.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Escherichia albertii"
                     /mol_type="genomic DNA"
                     /strain="BIA_35"
                     /host="Anser"
                     /db_xref="taxon:208962"
                     /plasmid="pEA35_4"
                     /geo_loc_name="Poland: North-East"
                     /collection_date="2016"
     gene            join(2393..2571,1..370)
                     /locus_tag="PS050_RS23325"
                     /old_locus_tag="PS050_23325"
     CDS             join(2393..2571,1..370)
                     /locus_tag="PS050_RS23325"
                     /old_locus_tag="PS050_23325"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_149494487.1"
                     /translation="MMIVNSCCPQLTTYTPTSCNNVRKPPQILFVEKGYILQENQSATA
                     PKDIPLVTSELFSCRAIAILGENSNWMAHIFEWPSTGQAHTKESMQKMLDTAAEMLGIQ
                     PVELPSCKVFIVCGSDPCDEIDEPLKSALSMYNINAEWFDGSDSTSPIYALAYQDEVGT
                     ANGDITEHHQTKEINSRQI"
                     /name="hypothetical protein"
     gene            complement(640..963)
                     /gene="mobA"
                     /locus_tag="PS050_RS23330"
                     /old_locus_tag="PS050_23330"
     CDS             complement(640..963)
                     /gene="mobA"
                     /locus_tag="PS050_RS23330"
                     /old_locus_tag="PS050_23330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_050571828.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            1247..1441
                     /locus_tag="PS050_RS23335"
                     /old_locus_tag="PS050_23335"
     CDS             1247..1441
                     /locus_tag="PS050_RS23335"
                     /old_locus_tag="PS050_23335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_024216200.1"
                     /translation="MNKQQQTVLNMAGFIKGQSLTLLEKLDALDADEQAIMCEKLHELA
                     EELQNSIQTRFEVENSTER"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1929..2090
                     /locus_tag="PS050_RS23340"
                     /old_locus_tag="PS050_23340"
     CDS             1929..2090
                     /locus_tag="PS050_RS23340"
                     /old_locus_tag="PS050_23340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072007783.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHELSPLAAATDNRIRGVRLE
                     VMRLLRLN"
                     /name="replication initiation protein"
     Typing          complement(973..1212)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.585"
                     /coverage="100.0"
                     /evalue="3.54e-121"
     Typing          1471..2001
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.327"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          complement(644..963)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.131"
                     /coverage="100.0"
                     /evalue="2.54e-62"
ORIGIN
        1 ggctattgcc atcttaggag aaaacagtaa ctggatggct catatttttg aatggcctag
       61 cacaggtcag gctcacacca aagagtccat gcaaaaaatg ctcgatactg cagcagaaat
      121 gctggggatt cagcctgtag aattaccttc ctgtaaagta tttattgtgt gtggctctga
      181 cccctgtgac gaaatagatg agccattaaa atctgcttta tccatgtaca acataaacgc
      241 agaatggttt gatggaagtg attctacatc tccaatttat gcattagctt atcaagatga
      301 ggttggtact gccaacggcg atataacaga acaccaccaa acaaaagaga tcaattccag
      361 acagatataa gtacgtgaaa tagtagattc acagctaaca ggagtagtcc tcagacatca
      421 tttaccataa tcccttaatt gtacgcacca ctgaaacgcg cagaacgcga ttctgacgca
      481 gacaccgaaa aaagagatgt tataccccgg aaaaacagga aggatgcgtc aggatcgttt
      541 tcggctcgct gcatagctat gcatgaaagc gaatggtgat cactttggga gcttacggtg
      601 ttcataccgt ctgttttcga cagttttctc tccgggaagc taatctgcca taagcctgga
      661 taacagggca cggtgatact ccgtaatagc gatcagcacc tccgcatact ccctgtcccc
      721 gacacgcttg ccgtcgataa agagtttttt ctgtaacgcc cccagtctca taacttcttt
      781 cagtacccga tcatcagtca gtgagttaac gttcttaccg agagctgccg cccgtaaaaa
      841 accggaaacg gtcttgccgc attcagcagc cctttttctg atttcctggt cttcttctgc
      901 cgtcagacga acagggcgac tgatagcccg cctgcgcgta ttacttccac ttcgttttgt
      961 cataaaacat ggctccgtat ctgacatggg tgtcggggca aagccctgac tagggtaatt
     1021 gtaatagcgt gcatgtatgc gcggtataac aattgcacat cctgtcctgt cagcaagctc
     1081 gaattaccga caaaggcacg gcagtttttt ggcagtgaca gtgttgtctg acagaaatgc
     1141 ccgtcaaaaa gccaggcggc agaatagcgg cagcaaaaca gtcgaaaagt ggtacgcgat
     1201 ataatcacat aacgcagatg acagacagaa cggagaaaac gaaaagatga acaagcagca
     1261 gcaaaccgta ctcaacatgg cgggattcat aaaaggccag agcctgacgc tgctcgaaaa
     1321 actggacgca ctcgatgctg acgagcaggc catcatgtgt gagaagctgc acgaactcgc
     1381 agaagaactc cagaacagca tacagacacg ctttgaagta gaaaatagca cagagagata
     1441 acgctcagga caggatgccg aacggcacaa cagagcgtaa ggagaaaatg tcacataagg
     1501 cgctcttccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact gcggcgagcg
     1561 gtgtcagctc actcaaaggc ggtaatgcgg ttatccacag aatcagggga taacgccgga
     1621 aagaacatgt gagcaaaaag ccgaaggcag ggacaaaggc cgcgcagatg gcgtttttcc
     1681 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1741 acccgacagg actataaaga taccaggcgt ttccccccgg aagctccctc gtgcgctctc
     1801 ctgttccgac cctgccgttt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1861 cgctttctca tagctcacgc tgttggtatc tcggcccggt gtaggtcgtt cgctccaagc
     1921 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1981 gtcttgagtc caacccggta agacacgaat tatcgccact ggcagcagcc actgataacc
     2041 gaattagagg agtaagactt gaagtcatgc gcctgttaag gctaaactga aagaacaagt
     2101 tttggtgact gcgctcctcc aagccagtta cctcggctca aagagttggt agctctgaga
     2161 accttcgaaa aaccgccttg cagggcggtt ttttcgttta catagcaaga gattacgacg
     2221 agaaggaaat gatctcaaga agaataaccg gaaccgtcaa gcctttcagg cttaccaacg
     2281 caagtaatta aaacatcatt aaagcaacga tggtgcagct ctgcctgcat attcagccac
     2341 ataccttatg tgtgtaaact gtaaattaga cggtaaacta tattgcaaaa acatgatgat
     2401 agttaattcc tgctgccctc aactcaccac gtatacacca acaagctgca acaatgtaag
     2461 aaagccacct caaatattat tcgttgagaa ggggtatatc ctccaagaaa accagtctgc
     2521 cactgccccc aaagatatcc ctttggttac gtcagagctt ttctcatgca g
//
