LOCUS       NZ_CP117598             6761 bp    DNA     circular CON 25-MAR-2024
DEFINITION  Escherichia albertii strain BIA_35 plasmid pEA35_3, complete
            sequence.
ACCESSION   NZ_CP117598
VERSION     NZ_CP117598.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33094105
            Assembly: GCF_028622475.1
KEYWORDS    RefSeq.
SOURCE      Escherichia albertii
  ORGANISM  Escherichia albertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Leszczynska,K., Swiecicka,I., Daniluk,T., Lebensztejn,D.,
            Chmielewska,S., Leszczynska,D., Gawor,J. and Kliber,M.
  TITLE     Escherichia albertii as a potential enteropathogen in the light of
            epidemiological and genomic studies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6761)
  AUTHORS   Leszczynska,K., Swiecicka,I., Daniluk,T., Lebensztejn,D.,
            Chmielewska,S., Leszczynska,D., Gawor,J. and Kliber,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2023) Department of Microbiology, Medical
            University of Bialystok, 2C Mickiewicza Street, Bialystok, Podlasie
            15-222, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. v1.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 321x
            Sequencing Technology :: Illumina Miseq/Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028622475.1-RS_2024_03_25
            Annotation Date                   :: 03/25/2024 01:35:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,670
            CDSs (total)                      :: 4,558
            Genes (coding)                    :: 4,308
            CDSs (with protein)               :: 4,308
            Genes (RNA)                       :: 112
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 92 of 250
            Pseudo Genes (incomplete)         :: 144 of 250
            Pseudo Genes (internal stop)      :: 61 of 250
            Pseudo Genes (multiple problems)  :: 41 of 250
            Pseudo Genes (short protein)      :: 3 of 250
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117598.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6761
                     /organism="Escherichia albertii"
                     /mol_type="genomic DNA"
                     /strain="BIA_35"
                     /host="Anser"
                     /db_xref="taxon:208962"
                     /plasmid="pEA35_3"
                     /geo_loc_name="Poland: North-East"
                     /collection_date="2016"
     gene            complement(join(5531..6761,1..1406))
                     /locus_tag="PS050_RS23305"
                     /old_locus_tag="PS050_23305"
     CDS             complement(join(5531..6761,1..1406))
                     /locus_tag="PS050_RS23305"
                     /old_locus_tag="PS050_23305"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0003677 - DNA binding [Evidence IEA]; GO:0016787 -
                     hydrolase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="WP_052512605.1"
                     /translation="MAKKPKSAGREKLLFNKLKQFDEPGLFHDNYQTPDYIQANLKDIL
                     RPYQHSALRYLHYTQRKRDDALLHYRHLLFHMATGAGKTMVMAGTILYLFKEFGYQNFI
                     FFVHTDAIIQKTRENLLNPQSPKYLFSHELEIDGEKITIAPVDIFPSVPERNTIYLKLS
                     TIHKMHDELNSYRENSITYEDLKEIPLVLLGDEAHHFNAGTKARGKSKTSPENEEQTWE
                     RTIENILDLRPDNRLFEFTATIDLANKDIGQKYRDKVVYQYDLKQFMSDGYSKKVMLLE
                     ANQNDSDKMLDAVLLSQYRKLTAADHGITGFKPVILFKSNKIAISKAKQEEFSQLIAAM
                     TPESIRRHLANKKLQLSSDTSIWHKVIQRYADSDLVTVTGQIQEDFNDFNLLNVNKSDL
                     LEENPVLLNTLEEVDNPVRAVFAVAKVNEGWDVLNLYDIVRISEQASSSKTSTDSEAQL
                     IGRGARYYPFIYDGQRSFTRRFDNSTKDLSVLEQLHYHTINEPAYIKTLHASLEQADID
                     VHQDGGGTVEHARLKEDFKKSAVYQAGKLYFNEVEEIERSSRNWETYSLETRFEIQYQI
                     ACEESLDNLTGTKGGIIKPEPLVLDERFYRKAMQRISFYALDNLKRFFPKLTGIREFIR
                     SDAYLGKLKITVSVPQSLDFTTVPAKDKLHLLENVLLRISENVRRNDQKVKGTYRFISQ
                     PVKEVIKDYSLHIDPSVAINQKITAAPTIGKKWYVYDNAILNQLEHRLIKMLEAFMPKL
                     KARYDDIYVLRNDEQSTRFKLTEFGGVRGFMPDFIMILTRHSDNTYWQVFLEPKGDDRL
                     LDDAWKERMLETLNDRERIVIDENEHVRLVGIKFFANSQMDVFVSDMQNKLNDGESLET
                     SSLSLPL"
                     /name="DEAD/DEAH box helicase family protein"
     gene            complement(1411..3276)
                     /locus_tag="PS050_RS23310"
                     /old_locus_tag="PS050_23310"
     CDS             complement(1411..3276)
                     /locus_tag="PS050_RS23310"
                     /old_locus_tag="PS050_23310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816428.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006306 - DNA methylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="WP_273819270.1"
                     /translation="MQTELIKELNKVLKAFPQFWNGEELQRSMVSDAISQKQPDLIKAL
                     VDNEKVRSVYGVDIDGIFIFDFDKLCGLLKYKEYWANSFTKYRNKVGLTSEGKYLDYSS
                     DVVLDFPFKDCILEGGMTKDNQGKDEIYYNEIIAMDEVDSLLSPKAFTNAKRYTKEGVK
                     ENITKFNDDENLIIKGNNLIALHSIKKLYAGKVKLIYIDPPYNTGSDSFKYNDRFNRST
                     WLTFMKNRLEVAKEMLSDDGFIFIQCDDNESAYLKVLCDSIFGEENYRNSIYWHRTYAG
                     KTVKSKLPWNTDTILFYSKKESSLLEPYTAELSAADIKSYNKDDFDGRGKYNTVSLQKT
                     SSPGPQTTYDYIDNTGKKWSCPEKGWRMTKEKLKALENDNRLYITDKTIREKYYLKERK
                     EIGKQIDNFWHDIGNLNRVSDVAFIDGQKTEKLMQRIISLASKENDIVLDFHLGSGTSA
                     AAAHKMGRRYIGIEQMDYINEITVPRLQKVIEGEQGGISKDVNWQGSGSFVYVELVELN
                     AYFIHEIQKAQSTEELEKLFAVMKTEAYLNYQVALENVLSAEYEVDGILHKVAFSELEL
                     HEQKQLLIEILDKNQLYVNVSDMEDSDLNISESDKAFTRSFYGME"
                     /name="site-specific DNA-methyltransferase"
     gene            4156..4350
                     /locus_tag="PS050_RS23315"
                     /old_locus_tag="PS050_23315"
     CDS             4156..4350
                     /locus_tag="PS050_RS23315"
                     /old_locus_tag="PS050_23315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041631.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELQNSIQIRFEAESETGT"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5015..5128
                     /locus_tag="PS050_RS23320"
                     /old_locus_tag="PS050_23320"
     CDS             5015..5128
                     /locus_tag="PS050_RS23320"
                     /old_locus_tag="PS050_23320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071588254.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLSPLAAATGN"
                     /name="replication initiation protein"
     Typing          complement(3642..3897)
                     /name="MOBF"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.248"
                     /coverage="89.0"
                     /evalue="2.69e-98"
     Typing          complement(4022..4120)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.0"
                     /coverage="100.0"
                     /evalue="1.35e-41"
     Typing          complement(4431..4516)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="8.58e-38"
     Typing          4534..5087
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.434"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atgaacgggt agtaacgcgc acctcgccca ataagctgcg cttcgctgtc cgtgctggtt
       61 ttgctgctgc tcgcctgctc gcttatgcga acaatatcgt agaggttaag tacgtcccag
      121 ccttcattca ccttcgcgac agcaaagacg gctctcactg gattatcgac ttcttccagt
      181 gtattcagca ggacggggtt ttcctccagc aggtctgact tgttgacgtt cagcaggtta
      241 aagtcgttaa agtcctcctg aatttgtcca gtcaccgtca ctaaatcact gtcagcgtaa
      301 cgctgaatca ctttatgcca gatactggtg tcagagctga gctggagttt tttgttagcc
      361 agatgacgtc tgatgctctc aggtgtcatg gcggcaatca gttgcgaaaa ctcctcctgc
      421 ttcgcctttg atatcgcaat cttattcgac ttgaacagaa tgaccggttt aaagccggtg
      481 atgccgtgat ctgctgccgt cagtttacgg tactggctta acagtaccgc atcgagcatc
      541 ttgtcgctgt cgttctggtt tgcttccaac agcatgacct ttttcgagta accgtctgac
      601 atgaactgct tcagatcata ctgatatacc accttgtcac ggtatttctg tccgatatct
      661 ttgtttgcaa ggtcaatggt agcggtgaac tcgaacaggc ggttatccgg tcgcaggtcg
      721 aggatgttct caatcgtacg ctcccaagtc tgctcctcgt tctccggtga ggttttcgac
      781 ttgcctctag ctttggtgcc ggcgttgaag tgatgcgcct cgtcaccgag tagcacaagc
      841 gggatttctt ttaaatcctc gtaggtaatg ctgttctctc gatagctgtt cagctcatcg
      901 tgcatcttat ggatagtcga gagcttgagg taaatcgtgt tgcgttccgg tacggacggg
      961 aatatatcta ccggtgcgat ggtgattttt tccccgtcga tttccagctc atgactgaac
     1021 aggtacttcg gcgattgcgg gttaagtagg ttctctcgcg ttttctggat aatcgcgtcg
     1081 gtatgtacga aaaagatgaa gttctgatac ccgaactctt taaatagata gagtatcgtt
     1141 cctgccataa ccatcgtttt ccctgcaccg gttgccatgt gaaacagcag gtgacggtaa
     1201 tgcagaagcg cgtcatcacg tttacgttgc gtgtaatgca ggtaacgcag tgcgctgtgc
     1261 tgatagggac gcaggatatc tttcaggttt gcctgaatat agtccggcgt ctgatagtta
     1321 tcgtggaaca gtcctggttc atcgaactgc ttcagcttgt taaacagcag cttctctcta
     1381 cctgctgact tcggtttctt cgccatgccg tcactccatt ccgtagaagc tgcgggtaaa
     1441 ggctttatca ctctctgaaa tgttcaggtc gctgtcctcc atatcagaga cattcacgta
     1501 gagctggttc ttatctagga tttcaatcaa aagctgcttc tgctcatgca gttccagttc
     1561 actgaatgct accttatgga ggatcccatc cacttcgtac tcagcggaca gcacattttc
     1621 cagagccacc tgatagttga gatatgcctc tgtcttcatc acggcaaaga gtttttcgag
     1681 ttcttccgtg gactgcgctt tctggatttc atgtatgaag taagcgttca gttccactag
     1741 ttcaacgtag acaaagctgc cgctcccctg ccagttgacg tcttttgaga tacctccctg
     1801 ctcaccctca atgacttttt gcaaacgtgg cacggtgatc tcattgatgt aatccatctg
     1861 ctcaataccg atatagcgac gtcccatttt atgggctgct gctgcgctag ttccgctgcc
     1921 gagatgaaag tccaagacga tatcattttc tttggaagca aggctaatta tccgttgcat
     1981 taatttttca gttttttgtc catcaataaa agcaacatcg gaaaccctat ttaaattccc
     2041 aatgtcatgc caaaaattat ctatttgttt cccgatctct tttctttctt taagataata
     2101 tttttctcgt attgttttgt cagtgatata tagcctattg tcgttttcca aagcctttaa
     2161 cttttccttt gtcattctcc accctttttc agggcaactc catttcttac ctgtattatc
     2221 tatataatca taagttgttt gcggccctgg agagcttgtt ttttgcaagc taacagtgtt
     2281 gtatttaccc cttccatcaa aatcatcctt gttataggat ttaatgtcag cagcactaag
     2341 ttctgctgta tatggctcta ataatgaact ttctttttta gaataaaaca atattgtatc
     2401 tgtattccat ggcaacttac tttttacagt tttccctgcg taggttctat gccaatagat
     2461 tgagtttcta taattctctt ctccaaaaat tgaatcacat aaaactttaa gatatgctga
     2521 ttcattatca tcgcattgaa taaaaataaa gccatcatcg gaaagcattt ctttagccac
     2581 ctcaagccta tttttcatga atgtcaacca tgttgagcga ttgaatcgat cattatactt
     2641 aaagctatca ctccctgtat tgtaaggcgg gtctatataa attaacttta cttttcctgc
     2701 gtaaagtttt tttattgaat gtagtgcaat gagattgttt cccttaatta ttaaattttc
     2761 atcatcgtta aatttagtga tattttcttt taccccctct ttggtatatc gcttagcatt
     2821 agtgaacgcc ttgggagaaa gcaaagagtc aacttcatcc attgctatga tttcattata
     2881 ataaatctca tcttttccct gattatcttt ggtcattcca ccttcaagaa tacaatcctt
     2941 gaaaggaaaa tccagcacca catcagaact gtaatcaagg tatttacctt cgctagttaa
     3001 tcccactttg ttccggtatt tagtaaagct attagcccaa tattctttgt attttagaag
     3061 accacataat ttgtcaaaat caaagataaa aatgccatca atatccacac cataaacaga
     3121 gcggactttt tcattatcga ctaatgcctt gataagatct ggctgctttt gactgatagc
     3181 gtcactcacc attgaacgtt gtaattcctc tccattccag aattgcggga acgcttttaa
     3241 aaccttgtta agttctttta ttaattcggt ctgcatgtca tggccttatg actgttaaat
     3301 attggaatga ttgtaaggta tagcgttcgg cgctggaacc caacagcgat gagttggatt
     3361 cagttgcggg aaaatgcacc agaacgagta gtatttaaca taaacgctct tgcgcgcacc
     3421 gctaaaacgc gctgaacgcg attctgccgc aaacaccgca aaaagagatg ttttccccgg
     3481 ggtaaaccgg aaaaatgcgt caggatcact ttcagctcac tgcatagcta tgcatgaaag
     3541 tgaatgacga tcggtttggg cgcatacagt acccatgaga cacattttcg gacattttca
     3601 gttcgtgaat ccctgactca ttgctacata tctgaacaga cacccctagc agcgccccca
     3661 ggggcgtcct gtaaaaaaac acaccgcgcc gctagcagca cccctaatat aagatgatgt
     3721 tttttatgaa aatagtcagt accaccccta aaaagcggtg tcggcgcggt gttgtagctg
     3781 cgccaacacc gcttttttaa atatcacaaa gagagcaaga agaactaatt tgtcataact
     3841 ctctatttat aaagaaaaat cagcaaaaac ttgtttttgc gtggggtgtg ggcatttcag
     3901 gcatgagaac agactcgcgt ttttccggag tatcagcgga attattgatt aagaaacact
     3961 cgacgcagag cgaaatttta tgcaacagcg gctgcactgc acagggatta tttatcagtt
     4021 gatatgctgt gccatgatac gtcctaaaga aacaggacag gatgtgcaat tgtaataccg
     4081 tcgcccgcaa cgtcttacaa ttaccacctg ctcagggtcc ccccgacacc cggaactgac
     4141 ggagaacaac ggaaggtgaa caaacaacaa caaactgcgc tgaatatggc gcgatttatc
     4201 aaaagccaga gcctgacact gcttgaaaaa ctggatgctc tggatgccga cgaacaggcg
     4261 gccatgtgcg aacgactgca cgaactcgca gaagaactcc agaacagcat ccagattcgc
     4321 tttgaggccg aaagtgaaac aggaacataa cgaagctgac ggaggcggtc acggctttcc
     4381 cgtgggcgtt ttcgtcagca gacaagcccg tcagggcgcg tcagggggtt ttagcgggtg
     4441 tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat
     4501 gcggcatcag tgcagattgt atgaaaagta caccataacc gatgtgaaat gccgcacaga
     4561 tgcgtaagga gaaaatgcaa cttccgacgc tcttccgctt cctcgctcac tgactcgcta
     4621 cgctcggtcg ttcggctgcg gcgagcggta tcaactcacg caaagacagt aattagatta
     4681 tccaccgaat caggggataa ggccggaaag aacatgtgag caaaagagca ggaaccggaa
     4741 aaaggccgcg ccgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     4801 tcgacgctca agtcagagtc ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4861 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     4921 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     4981 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     5041 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     5101 gccactggca gcagccactg gtaactgatt tagaggagtt tgtcttgaag ttatgcacct
     5161 gttaaggcta aactgaaaga acagattttg gtgactgcgc tcctccaagc cagttacccg
     5221 gttagataat ctgccaggat tagctaacct tcgaaaaacc acctgccagg gtggtttttt
     5281 cgtttacagg ctgcagatta cgcgcagaaa aaaaggatca caagaggatc cttgaatctt
     5341 ttccccctca tccccccccc caggcataaa catagcgaac ggtttttaac caccattcaa
     5401 aactaatgat tcgcaacagc aagaacaaac aatgcaacca ttacaggagc aatcatcacc
     5461 tccctgggag gtagttcgag ataacaataa aataacggag cgtaatgctc cgttatttta
     5521 ttgtcgtgac ttacagtggt agggagagcg aggaggtttc gagcgattct ccatcattca
     5581 gcttgttctg catgtcgctg acaaaaacgt ccatctggct attggcaaag aatttaatcc
     5641 caaccagtct gacgtgctca ttttcatcaa ttacaatgcg ctccctgtca ttcagtgttt
     5701 cgagcatccg ttctttccat gcgtcatcca gcagacggtc atcccccttc ggttcaagga
     5761 acacctgcca gtaagtgttg tctgaatggc gcgtcagtat catgatgaaa tctggcataa
     5821 acccacgcac gccgccgaac tccgtgagct taaagcgcgt tgactgctcg tcgttacgca
     5881 gtacgtaaat atcgtcataa cgcgctttta gcttcggcat gaatgcttcc agcattttta
     5941 tcagacgatg ctcaagctga tttagtatcg cattatcgta cacgtaccat tttttaccaa
     6001 tggtgggagc ggcggtgatt ttctggttaa tggctaccga cgggtcaata tgcaaactgt
     6061 aatctttaat cacctctttc accggctggc tgataaatcg gtacgtgcct tttaccttct
     6121 ggtcattgcg acgcacattc tctgagatac gcagcaaaac gttttccagt aggtgcagct
     6181 tatcttttgc cggtacggtg gtgaaatcca gtgactgcgg tacgctgacg gtgattttca
     6241 gcttaccaag ataggcgtca ctgcgtatga actcccgaat acctgtcagt ttcgggaaga
     6301 agcgtttcag gttatctagc gcgtaaaagc tgattctttg catcgccttg cggtaaaaac
     6361 gctcgtccag taccagtggt tccggtttta tgatcccccc ctttgtgcct gtcaggttgt
     6421 ccagcgactc ctcgcaagcg atctgatact gtatttcaaa gcgtgtttcc agcgagtagg
     6481 tttcccagtt tcggctgctt ctctcaatct cttccacttc gttaaagtac agcttacccg
     6541 cctgatacac ggcggacttt ttaaagtctt ctttcaatct ggcgtgttca accgtacctc
     6601 cgccatcctg atgcacgtca atgtccgcct gctccagaga ggcgtgcagt gttttgatgt
     6661 acgccggttc attgatggtg tgatagtgca gttgctccag tacgctcaaa tccttcgtac
     6721 tgttatcaaa acggcgggtg aagctacgtt gaccgtcata a
//
