LOCUS       NZ_CP117597             7076 bp    DNA     circular CON 25-MAR-2024
DEFINITION  Escherichia albertii strain BIA_35 plasmid pEA35_2, complete
            sequence.
ACCESSION   NZ_CP117597
VERSION     NZ_CP117597.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33094105
            Assembly: GCF_028622475.1
KEYWORDS    RefSeq.
SOURCE      Escherichia albertii
  ORGANISM  Escherichia albertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   Leszczynska,K., Swiecicka,I., Daniluk,T., Lebensztejn,D.,
            Chmielewska,S., Leszczynska,D., Gawor,J. and Kliber,M.
  TITLE     Escherichia albertii as a potential enteropathogen in the light of
            epidemiological and genomic studies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7076)
  AUTHORS   Leszczynska,K., Swiecicka,I., Daniluk,T., Lebensztejn,D.,
            Chmielewska,S., Leszczynska,D., Gawor,J. and Kliber,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2023) Department of Microbiology, Medical
            University of Bialystok, 2C Mickiewicza Street, Bialystok, Podlasie
            15-222, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. v1.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 321x
            Sequencing Technology :: Illumina Miseq/Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028622475.1-RS_2024_03_25
            Annotation Date                   :: 03/25/2024 01:35:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,670
            CDSs (total)                      :: 4,558
            Genes (coding)                    :: 4,308
            CDSs (with protein)               :: 4,308
            Genes (RNA)                       :: 112
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 92 of 250
            Pseudo Genes (incomplete)         :: 144 of 250
            Pseudo Genes (internal stop)      :: 61 of 250
            Pseudo Genes (multiple problems)  :: 41 of 250
            Pseudo Genes (short protein)      :: 3 of 250
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117597.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7076
                     /organism="Escherichia albertii"
                     /mol_type="genomic DNA"
                     /strain="BIA_35"
                     /host="Anser"
                     /db_xref="taxon:208962"
                     /plasmid="pEA35_2"
                     /geo_loc_name="Poland: North-East"
                     /collection_date="2016"
     gene            complement(5758..6618)
                     /locus_tag="PS050_RS23300"
                     /old_locus_tag="PS050_23300"
     CDS             complement(5758..6618)
                     /locus_tag="PS050_RS23300"
                     /old_locus_tag="PS050_23300"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-116"
                     /protein_id="WP_000027050.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-116"
     ARG             complement(5762..6618)
                     /name="blaTEM-116"
                     /gene="blaTEM-116"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-116"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          4813..5324
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.127"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
       61 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
      121 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
      181 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
      241 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata
      301 cgactcacta tagggcgaat tgggtaccgg gccccccctc gagtaaataa aaaaatgact
      361 agaatattag ttattaaaat tttaaaatga ggaaacgaac taagtgctaa ttcatagagc
      421 aagaagcttc agaaggttac tcctcagaag aggatgacga tccaaagttc gagattaaca
      481 gaaaagaggc tgaaaggcaa cttaaggaaa tccaatctag gagggcacag gcaaaggaaa
      541 gacttaataa acgattacaa gaattgatgg agcaagacgc cattgatgag acttaaaata
      601 actcagaata ttcttcaaat gttatcgacc aagatgacga agatcaagac aacgatgatg
      661 acgatgatac tcttaaggga gggccaaaat tgaatgatcc taaggtttgg agatttaaat
      721 gtggaagatc tatattagaa gtacagagaa ttttggaaat ttctaatttg cttccaaaga
      781 attctcccat cgtttctgtg ttttacacac ctaatgtcaa gggttttata ttcttcgaat
      841 catttttcga aaacgatgtg aaagaattta tgcggtctat aatgtacggt taacctattt
      901 atatctaacc cgaggactgc gattcattgc tggaaattaa aaagaacaat aacattgaag
      961 aaggccaatg ggtcagagtt agacaacaca acatttactc tggagatctt gctagagtca
     1021 tgtaagtgga tcaagagaga aaacttatca aactcaaagt agttcccaga caaaaattac
     1081 taatgaaatc tcttgaagaa gaggaagaaa ataaaaagaa gaattccaaa aagaataatg
     1141 ccaatgcaga tcaatctcgc ttccaaatga aagaagaaga aggtgaacca cccattgaag
     1201 acaatgagcc tttctaaaga ggcagcaaat tcaagttctt aaaaagagcc aagtatcttc
     1261 aagaacaaag gaaatacatt cgtggtccta aaatacctat ttagatgacc aagaaatcca
     1321 ttaatgatga caatgacgaa cctacttccc aattcttcta tactaccatt agagatgaat
     1381 ggacatctgc taaaaaggat ggatttgaaa tcatcactct tccagttcat caggttctta
     1441 ctggcaatat caaacccact gtagaagact tgtaatactt cttccctgat gtagttgatc
     1501 gaaagattct tgtctaatag gctaaaagag aaatacttag aagagtggat gtacagttta
     1561 aagaaggaca aaaagtcaga ttaatagggg aagatgatat caataaaggt cctaaattca
     1621 aaataatgaa aatatttgat gattaaatga tagaacttat gtgtaagaaa gataatcgag
     1681 aatacacata tctagttcat gcatcagaaa tacgattagc ttttaaatta tattaggaag
     1741 ccaaagttat agatggtcct cacaaaggag acgtaggtgt tattatatgt atcaaataag
     1801 gttatgttgt gctttcaaat taacatggaa ctttcaaggt acctcacagt gtgttataat
     1861 ttggacataa gaatttttaa actgataatt ctttagtcaa atttgggaac aatgactttt
     1921 aaatcggctg tgttattcaa taaaagctgt aatctgctgt tgttttggat atcaataatg
     1981 aggtttaaga aataagaaat gagaaactag aaattctcac aataaatgga gttgaaatga
     2041 atgaataagg tgaaacattc agaatcaatg ataatgtgat aatcatatca ggttagtatg
     2101 ctaataaatt tggattagtc aaacattgta taaatggtaa gctatattta tttaaccatt
     2161 tgtttcctta ctaaattatt cttgaaaatg caaataactg caaaatggta tttagtaaac
     2221 aaaacaaacc aaaatagaat gattagatgt gtttgactgg ataaatttgt caactaaaaa
     2281 ttggataatg gtaaggatat cgaggataaa ttgtttaaat aaaatctgga tatttaatag
     2341 tttaattgtc tgctaataat acgaaggtga aggtgtcaga aaaagatatt cttttattag
     2401 ctagctcagg aggaggatca ggaggatcag ggggaatgaa taattctaga ggagaagaac
     2461 ttttcactgg tgttgttcca attcttgttg aacttgatgg tgatgttaat ggacataaat
     2521 tttctgtctc tggtgagggt gaaggtgatg caacttatgg aaaattaacc ttaaaattta
     2581 tttgcactac tggaaaatta cctgttccat ggccaacact tgtcactact ttaacttatg
     2641 gagtccaatg tttttcaagg taccctgacc acatgaaaca acatgacttt ttcaaatctg
     2701 ccatgccaga aggatatgtc caagaaagaa ctatattctt caaagatgat ggaaactaca
     2761 agacaagagc tgaagtcaaa tttgaaggag atacccttgt caatagaatt gagttaaaag
     2821 gaattgattt taaagaagat ggaaacattt taggacataa attggaatac aactataact
     2881 cacataatgt atacatcatg gcagacaaac aaaaaaatgg aatcaaagtc aacttcaaaa
     2941 ttagacacaa cattgaagat ggatcagttc aattagcaga ccattatcaa caaaatactc
     3001 ctattggaga tggaccagta cttttaccag acaaccatta cttatcaaca caatctacct
     3061 tatcaaaaga tccaaatgaa aagagagatc acatggtgtt attagagttt gtaactgctg
     3121 ctggaattac acatggcatg gatgaattat acaaagctag ctcaggagga ggatcaggag
     3181 gatcaggggg aggatccatg atactagaag aacaaaagcc cttcaccact gaagaactct
     3241 atgaataccg taaggtccta gtagactgga aatggcgagg tcctgatgcc atttactttg
     3301 ttctaggcta actatgcgta aataccatat attctatcaa tttaagaagg gaaccctaga
     3361 aagagaggcg attgtgcaaa gcaaaatata taagagtgtc tatatcctca gggagtctat
     3421 caggaaggct ccctcaactg atatggcatt atataataag gattaaatcg ccaccatttt
     3481 ggtctaaata attcacttag ttttagttaa aactcaagtc cattcataat tagatcaaag
     3541 aaataatttc taagccccct aagcgagaaa ataaaaagac catattttct gttgccaaat
     3601 atgtattatg cataaggatg ttaattttta gactgataac atccaaggaa cctaatccaa
     3661 tattcaactg ggagactact tgcctcaaga ctagagggat aagatcagag ctaaattgtc
     3721 tgagtatcta cagaaataac tttaataaaa ataataatag gatctttttt aggaggaaaa
     3781 tttgtaataa tgctaaaagc tattagttag agtggacaag tgcaataagt tagctgatgc
     3841 tcttgaaaac aatttataca acaaacatta ttaaacagta gcaccaggaa aaaattatag
     3901 ttctgatttg aaaaccttat accaagtata attaggatat aattcaagta cttgggcaaa
     3961 gacaaatcag ggagagtgct acacaagtta tttgaaggct tttttacagt gaattaagca
     4021 tgttaattat aactgaagga ttggttggat gaagaagtta ttagagagtt agaaaataga
     4081 aaaattgaaa ttaaatttga ctatcgaata ttagtctgaa atgaaagatg acgatgcaga
     4141 ttactataga tagattcgca tcaaggaaat gactcaatcg aaaggttagg aatgtccaag
     4201 atgtcataat aattatttat acataattga aactaaataa ataagaagag cagatgaacc
     4261 agctacaatc ttctacgaat gttttgcatg ttaacataga tacaaagttt gagtatttca
     4321 attaaaatta taattttgtt tctattataa cttaaatgat ctaccaagtt accaaatgct
     4381 atgattctga aatttaatat aaataaaata ggtatataag taaatagatt gatatttctc
     4441 atttaattat ttgctatcct tttttttcaa cggatcttat tacaccaact gctgcagccc
     4501 gggggatccg cccgggctag agcggccgcc accgcggtgg agctccagct tttgttccct
     4561 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     4621 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     4681 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     4741 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     4801 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4861 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4921 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4981 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     5041 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     5101 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     5161 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     5221 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     5281 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     5341 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     5401 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     5461 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     5521 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     5581 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     5641 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     5701 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     5761 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     5821 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     5881 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     5941 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     6001 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     6061 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     6121 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     6181 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     6241 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     6301 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     6361 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     6421 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     6481 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     6541 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     6601 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     6661 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     6721 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
     6781 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     6841 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     6901 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     6961 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     7021 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagcc
//
