LOCUS       NZ_CP117566             4936 bp    DNA     circular CON 25-MAR-2024
DEFINITION  Escherichia albertii strain BIA_7 plasmid pEA7_4, complete sequence.
ACCESSION   NZ_CP117566
VERSION     NZ_CP117566.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33094114
            Assembly: GCF_028622195.1
KEYWORDS    RefSeq.
SOURCE      Escherichia albertii
  ORGANISM  Escherichia albertii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Leszczynska,K., Swiecicka,I., Daniluk,T., Lebensztejn,D.,
            Chmielewska,S., Leszczynska,D., Gawor,J. and Kliber,M.
  TITLE     Escherichia albertii as a potential enteropathogen in the light of
            epidemiological and genomic studies
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Leszczynska,K., Swiecicka,I., Daniluk,T., Lebensztejn,D.,
            Chmielewska,S., Leszczynska,D., Gawor,J. and Kliber,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-FEB-2023) Department of Microbiology, Medical
            University of Bialystok, 2C Mickiewicza Street, Bialystok, Podlasie
            15-222, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. v1.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 263x
            Sequencing Technology :: Illumina Miseq/Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028622195.1-RS_2024_03_25
            Annotation Date                   :: 03/25/2024 01:45:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,343
            CDSs (total)                      :: 5,210
            Genes (coding)                    :: 4,989
            CDSs (with protein)               :: 4,989
            Genes (RNA)                       :: 133
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 101
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 221
            CDSs (without protein)            :: 221
            Pseudo Genes (ambiguous residues) :: 0 of 221
            Pseudo Genes (frameshifted)       :: 82 of 221
            Pseudo Genes (incomplete)         :: 156 of 221
            Pseudo Genes (internal stop)      :: 43 of 221
            Pseudo Genes (multiple problems)  :: 52 of 221
            Pseudo Genes (short protein)      :: 3 of 221
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117566.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Escherichia albertii"
                     /mol_type="genomic DNA"
                     /strain="BIA_7"
                     /host="Columba livia"
                     /db_xref="taxon:208962"
                     /plasmid="pEA7_4"
                     /geo_loc_name="Poland: North-East"
                     /collection_date="2015"
     gene            complement(911..1069)
                     /locus_tag="PS049_RS26765"
     CDS             complement(911..1069)
                     /locus_tag="PS049_RS26765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071777339.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            complement(1728..1919)
                     /locus_tag="PS049_RS26650"
                     /old_locus_tag="PS049_26650"
     CDS             complement(1728..1919)
                     /locus_tag="PS049_RS26650"
                     /old_locus_tag="PS049_26650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1984..2307
                     /locus_tag="PS049_RS26655"
                     /old_locus_tag="PS049_26655"
     CDS             1984..2307
                     /locus_tag="PS049_RS26655"
                     /old_locus_tag="PS049_26655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955996.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2516..>2947
                     /locus_tag="PS049_RS26660"
                     /old_locus_tag="PS049_26660"
                     /pseudo=""
     CDS             2516..>2947
                     /locus_tag="PS049_RS26660"
                     /old_locus_tag="PS049_26660"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2985..3503
                     /locus_tag="PS049_RS26665"
                     /old_locus_tag="PS049_26665"
     CDS             2985..3503
                     /locus_tag="PS049_RS26665"
                     /old_locus_tag="PS049_26665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_112016408.1"
                     /translation="MSNLLRTGAEFEKKLKERAESTEKMLNDEFSRLGESVSEAVTSNE
                     TKIKDAIALFTTSTEESLKKHREGVKEAMMQHRKDMLKLAGSSGMRLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRVQEETLRKLNAKTWGVEFVQDGRRKFLVLPYGKSAEVTHYQ
                     GKDWIQLTE"
                     /name="MbeB family mobilization protein"
     gene            3510..3743
                     /locus_tag="PS049_RS26670"
                     /old_locus_tag="PS049_26670"
     CDS             3510..3743
                     /locus_tag="PS049_RS26670"
                     /old_locus_tag="PS049_26670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_096987281.1"
                     /translation="MTELETHLLNALKQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSGQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4354..4650)
                     /locus_tag="PS049_RS26675"
                     /old_locus_tag="PS049_26675"
     CDS             complement(4354..4650)
                     /locus_tag="PS049_RS26675"
                     /old_locus_tag="PS049_26675"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_138650211.1"
                     /translation="MERKHKGKCPFCNSEMAPEVIEKNTIRRDKCKCTTCGEIIYKCRN
                     IFCNDYAKGGLLYDDELCPPCGEGLLKAVKEFPDKYRAAIQKVVEEKNREKNN"
                     /name="hypothetical protein"
     gene            complement(4725..4880)
                     /locus_tag="PS049_RS26680"
                     /old_locus_tag="PS049_26680"
     CDS             complement(4725..4880)
                     /locus_tag="PS049_RS26680"
                     /old_locus_tag="PS049_26680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010940654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="WP_072210546.1"
                     /translation="MNDKWEFYLDNSKEWRWRRTASNGRIVGASSQGYVNKSDCIDNAR
                     RHGYNG"
                     /name="DUF1508 domain-containing protein"
     Typing          1566..1650
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.674"
                     /coverage="100.0"
                     /evalue="6.569999999999999e-34"
     Typing          complement(998..1555)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2298..3841
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.34"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atcatttcat ttttttttct aataagcgtg ttacaactgt actgacgacg atacttaagg
       61 ctgttgtccc taatgcgccg atacttctac ctatgttcga catgcatgac gggcataatt
      121 catcatcata taaatgaccg cccttagcgt aattctcaca tcctggagta cgacatacgt
      181 aaattattga tttacaatca gaacactcac acttatctcg ccgtatcttg ttttcttgaa
      241 ttacacgggc attgatttcc tttttacaaa aaggacacac tcctttaggt tttaagtcca
      301 ttagggttta cctaatagca ctacaactta ttaattttga acgacattta gggcaataat
      361 caggtgtccc aaatccagct ctactggtcc attcgtggcg gcagtttaag catttagccc
      421 gatagatgac gatacctgcc ataaaaatcc cctttttatc atacactaat gttgatttat
      481 tatattctga acataataaa tgtaaagtat actatgtgat aaacgtaatg cattgacttt
      541 attgatatta aaaatattta ttagagtgct aataatggta ttttttttga tttttttatt
      601 aaaaacaata tgttgtatat gtgttggtgt cgtttgatta cgttcaataa atgcgtgtat
      661 caatattaga tatacaaaaa tgaagttaga aggtggctgg gagttttagg agatttgccc
      721 tccttcagta aaaagatcaa aggatcctct tgagatcctt tttttctgcg cgtaatctgc
      781 tgcctgtaaa cgaaaaaacc gcctggggag gcggtttagt cgaaggttaa gtcagttggg
      841 gactgcttaa ccgggtaact ggcttggtgg agcacagata ccaaatactg tccttctagt
      901 gtagccgcag ttaggccacc acttcaagaa ctcttaatat ctcaatccac cttgtccagt
      961 taccagtggc tgctgccagt ggcgttaagg cgtgtcttac cgggttggac tcaagacgat
     1021 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1081 tggagcgaac gacctacacc gaactgagat accaacagcg tgagctatga gaaagcgcca
     1141 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     1201 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     1261 gccacctctg acttgagcgt cgatttctgt gatgctcgtc aggggggcgg agcctatgga
     1321 aaaacgccag cggcgtggcc ttttccgggt tcttgttttt tgctcacatg tttcctttgg
     1381 cgttatcccc tgattctgtg gataaccgca ttaccgtctt tgagtgagct gataccgctc
     1441 gccgcagccg aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa aagcgcctgg
     1501 acgcgcattt tctccttacg catctgtgcg gcatttcaca cccggcatgg ggcacttttc
     1561 atacaatctg cactgatgcc acatagttaa gccagtatac actccgctag cgctacgtga
     1621 ctggttcagg gcttcgcccc gaaaccccct gacgcgccct gacgggcttg tctgctcccg
     1681 gcatccgctc acagacaagc tgtgaccgtc tccgggagct gtatgtgtca gaggttttca
     1741 ccgtcatccc cgaagcgtgc gaggcagctg cggtaaagct cgtcggcgtg gtcgtgaagt
     1801 gattcacaaa tatcggcctg ttcatctgcg tccagttcgt tgagtttctc cagcagcgtt
     1861 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttagtcact
     1921 gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag
     1981 aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgctgtgag
     2041 ggtaaacaac tggcggtatg gatgcggcgg gtctgcctgg gtgagccggt tgcccgttcc
     2101 gggaagctgc caactttggc tccgccgtta ctgcgtcagc tggccgccat cgggaataac
     2161 ctgaatcaga cagcgaggaa ggtgaacagc gggcagtggt cttccggcga ccgggttcag
     2221 gtggtggccg cactgatggc catcgagaga gagttgcgca gcttacggca ggtcgtcagg
     2281 gaacagggag cgcgggatga tagttaagtt tcatgccagg ggaaaaggtg gtggcagtgg
     2341 tccggttgat tacctgctgg ggagggagcg taatcgcgaa ggcgcaacgg tgcttcaggg
     2401 taatccggaa gaagtccggg aactcatcga tgccacgcca tttgcgaaga aatacacgtc
     2461 cggtgttctg tcgtttgcgg agaaagaact gccaccggga ggacgtgaaa aagtgatggc
     2521 gagttttgag cgtgtactga tgcccggtct cgaaaaaaat cagtacagca tcctgtgggt
     2581 ggagcaccag gacaagggac ggcttgagct gaattttgtc attccgaaca tggagctaca
     2641 gaccggaaaa cgcctccagc cgtactacga ccgggcagac aggccgagaa ttgatgcctg
     2701 gcagacgctg gttaatcacc attacgggct gcatgacccc aacgccccgg agaatcgccg
     2761 gacgctgaca ctccctgata acctgccgga aacgaaacag gcgcttgctg agagcgttac
     2821 acgaggcctg gaggtgcttt accattccgg agagataaaa ggccgtcagg atgtgattca
     2881 ggcgctcaca gaggtggggc ttgaagtggt cagggtgacg cgaagcagta tcagcattgc
     2941 agacccgaac ggcgggagga atatcagact gaaaggagcg ttttatgagc aatcttttac
     3001 ggacgggcgc ggagttcgag aaaaagctga aagagagagc cgaatctaca gagaaaatgc
     3061 tgaacgacga attcagcagg ctcggcgaat ctgtaagcga ggctgtgacc tcaaacgaga
     3121 cgaaaatcaa agacgctata gccctgttca caacgtcgac agaggaatcg ctgaaaaaac
     3181 accgggaagg ggtgaaagag gcgatgatgc agcaccggaa ggacatgtta aagctggcag
     3241 ggagttcggg catgaggttg ctggggatag tctttctcct gtttaccgcg agtggcggga
     3301 cgctctggta tctcggcggg aggatacaga cgaacctgga agaaatcagg gtacaggaag
     3361 agacattgcg gaaactgaac gcgaagacat ggggcgtgga gtttgttcag gacgggagaa
     3421 ggaaatttct ggtccttccg tacgggaaat cagcagaggt gactcactat caggggaagg
     3481 actggataca actgacggag tgatacggaa tgacagagct ggaaacacat ttgctgaacg
     3541 ccttaaagca gctgcaacag gactatatgc agcggctgaa cgaatgggag agcgccttcg
     3601 tggaattgca gaagatgttt tcgcttacgc aacgggacaa cgcgatgctg aacgagcggg
     3661 tcatgcagtt gagtcagcag gtgcaacact tgagcgggca gacagaacgc ttgagccagt
     3721 tatacagcga gaacaggaga taagggatga acggctgatg caggagcgcg aacatgcatt
     3781 atccctggag cgggaacgcc agctggaaat acaggaacgc acactggatg gtccttcgtt
     3841 gtcgctgtga tggcgaaact atgaaaaatg gcaggttcgg tggattttga cgggctaatg
     3901 tggtctgcac catctggttg cataggtatt catacggtta aaatttatca ggcgcgatcg
     3961 cggcagtttt tcgggtggtt tgttgccatt tttacctgtc tgctgccgtg atcgcgatga
     4021 acgcgtttta gcggtgcgta caattacagg gttatgttaa attgatggtt tgataagtgt
     4081 tggaaataat taattccact cataaatata caagatatga aaaaaatatt gttcatatgg
     4141 tagaagagct atagggatat ttggtttcaa aatattcctt ttaggaaatg gattaactaa
     4201 aacaggtact aaaatacctg ttaaaaataa tgagagagat aactctctgt tagggcgtgt
     4261 gttggggata tagaaatatt tggtattgct tacgaaatcg attggttttc atgataatta
     4321 actaatatag ttatatggtt ttgtgcgcta ccattaatta ttcttttctc tattcttctc
     4381 ttctacaact ttttgaattg ctgctcgata cttatcaggg aactctttta ccgcctttaa
     4441 aagaccttca ccacatggag ggcacagttc gtcatcgtaa agtaatccgc cttttgcgta
     4501 gtcattacag aatatatttc ggcatttata aataatctct ccacaagttg tacatttaca
     4561 tttatctctg cggatagtgt ttttttctat aacttctgga gccatttcgc tattgcaaaa
     4621 agggcactta cctttgtgtt ttctttccat ggtatttttc tatttgttta tctctttgca
     4681 attgggattc ctgcttggaa tcccaacatg agaatcaatt tatattagcc gttgtagcca
     4741 tgacgacgtg cgttatcaat acaatcagat ttattgacat atccttgaga tgatgctcca
     4801 acaattcgcc cgttgcttgc agttctgcgc cagcgccact cttttgaatt atcgagataa
     4861 aactcccact tatcgttcat ttaggtttcc ttcttccagt tagatattta gtgatgattt
     4921 gtaccagcag gtaggt
//
