LOCUS       NZ_CP117502             2500 bp    DNA     circular CON 05-APR-2024
DEFINITION  Enterococcus faecalis strain CQHZJ21-185 plasmid pHZJ21-2K, complete
            sequence.
ACCESSION   NZ_CP117502
VERSION     NZ_CP117502.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33180039
            Assembly: GCF_036774845.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Tang,B. and Ni,J.
  TITLE     Complete genome sequence of Enterococcus faecalis CQHZJ21-185
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Tang,B. and Ni,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2023) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. NOV-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036774845.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 15:48:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,945
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,819
            CDSs (with protein)               :: 2,819
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 4 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117502.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="CQHZJ21-185"
                     /isolation_source="feces"
                     /db_xref="taxon:1351"
                     /plasmid="pHZJ21-2K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2021-11"
     gene            complement(24..122)
                     /locus_tag="PSC76_RS14715"
                     /old_locus_tag="PSC76_14690"
     CDS             complement(24..122)
                     /locus_tag="PSC76_RS14715"
                     /old_locus_tag="PSC76_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_146035423.1"
                     /translation="MIDKIFTLVIAPIFVGLVIELVSHWLDEKDDN"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(391..651)
                     /locus_tag="PSC76_RS14720"
                     /old_locus_tag="PSC76_14695"
     CDS             complement(391..651)
                     /locus_tag="PSC76_RS14720"
                     /old_locus_tag="PSC76_14695"
                     /inference="COORDINATES: protein motif:HMM:NF020567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_104885178.1"
                     /translation="MKPYDYTQTLKIEQLNELSKGTTARLISYLTNENIEIDNRNNFFA
                     LLYQQFKEMLNLNLFSLSDTELKRVEQYWQKMNDLVSTLSK"
                     /name="hypothetical protein"
     gene            complement(654..1622)
                     /locus_tag="PSC76_RS14725"
                     /old_locus_tag="PSC76_14700"
     CDS             complement(654..1622)
                     /locus_tag="PSC76_RS14725"
                     /old_locus_tag="PSC76_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_338468898.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVDDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VEEPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSSYSKTMFRLLKQFRTKGYAYFS
                     KEDFHELLDIPKSYRESNIDQYVLKPIRQELTAIFKGLAIKKKYGKGRGKPVIGYQFSF
                     KPEIKKADDFNKHAPTKEEQNRALAQADKQKEYEQQPTTIDDKILEAKRKVELLEQLKA
                     LEKKEKHSKEKEPISETNSSLSSSEKDDLLGELHNIFTKED"
                     /name="replication initiation protein"
     Typing          complement(761..1622)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.821"
                     /coverage="89.0"
                     /evalue="0.0"
ORIGIN
        1 gtgtgagtta gagcaacttg cttttagtta tcgtctttct cgtctaacca atgagaaact
       61 agttcaatga ctagcccaac aaaaattggt gcgataacca atgtgaagat tttgtctatc
      121 aagagaaaca ccccctctca ggggcaagtt gccatttttg agtatagcac aatgtcagaa
      181 taggagaagg gcaagcatcg ggtgtgtgcg cacacaccac cattccaagt agaatttttg
      241 cttgcctttt gaaaaaggca agcatcggat agtgcatgca atatcctaaa attccaaatg
      301 gaatgcttgt taggtttcac ctaataccga taaaaagcct gaagcaaaaa acgtcttttg
      361 cttcaggctt ttttgtcgct tcgctcgaat ttattttgaa agagttgaaa caaggtcatt
      421 cattttttgc cagtattgtt ctactcgttt tagttcagta tctgacaggg aaaataagtt
      481 gagattcagc atttctttga attgctgata caacaaagca aaaaaattat tccggttatc
      541 aatttcgata ttttcgttcg tcaaataact aatcagtcga gcggttgtac cttttgataa
      601 ttcgttcaat tgttcgattt tgagtgtttg agtgtaatcg tagggcttca aaatcaatcc
      661 tccttcgtaa aaatattgtg taattcaccg agtaaatcat ctttttcaga agatgaaagg
      721 gaagaattag tttcggaaat aggctctttt tcctttgagt gtttttcttt tttctctaag
      781 gctttgagct gttcaagcaa ctcaactttt ctctttgctt ctaaaatttt atcgtcaatc
      841 gttgttggtt gttgttcgta ttctttttgt ttatctgctt gagctaaagc cctgttttgt
      901 tcttctttgg ttggggcatg tttattaaag tcatctgctt tttttatttc aggcttaaag
      961 gaaaattgat agccaattac aggctttcca cgaccttttc cgtatttctt tttgattgct
     1021 agccctttaa agattgcggt taattcttgt ctaataggct ttaaaacgta ttgatctata
     1081 ttactttctc tataactttt tggtatgtct agaagttcat gaaaatcttc tttggaaaaa
     1141 taggcatatc ctttggttcg gaattgtttt aacaagcgga acatcgtttt agagtagcta
     1201 ctttgtagct cgttgaattg ttggagagaa taacgtaccc actcgtctaa attatttaac
     1261 aaaggcagag ctttttcgta aattcgtata tctacataag gctcttcaac gtcaccattg
     1321 attttaaatt ccgtaaacat cacaaacata tcgtaagaaa gcccagactc acttctacga
     1381 ccaaagcgta acgataagat tttctcgtac gttgccttta aatcatctac aaaccgcacg
     1441 ttagcagtcg ctttatagtt actcagatct ttcagttggt caaacgtaaa gcggacggtt
     1501 ttatcgcctt tttcacgcat tctggacacg atagagaaaa acaaattcat ttcaacagga
     1561 gtaaatttcc gtaacggaat agtattcaat tcatgatgat atttcacgat ttcatttgcc
     1621 ataaaacttc acctcttttt ataagtatgc acaaataagc ataccatgcc tttaaaaaca
     1681 tgtcaaaaca gacaaaacca tgcctataaa cagacaaaac catgcctata aacagacaaa
     1741 accatgccta taaacagaca aaaccatgcc tataaactca ccaatccctt gggagagtaa
     1801 ggacacagcc gacggtaaag aggtttaaaa gaggtttaaa aagagattat aaaagagggg
     1861 gggcaaaatt tcccgcctaa aaaagctcat ttttttagat aaacaggttc gcgaattttg
     1921 caaaactgca aataaaaaac caaaagacaa aaaaggagtg atcgctacgc tctcacattt
     1981 tacttgcagc ggtcactccg ttccctcgct aaaatcaaaa ccggcaatta aggtatcctt
     2041 tgggcaggct ctgattgctc gtataagcct ctctaagaca tttttagtgg ttacagccaa
     2101 tcttgttcct caagcgcctt tcaggcggtc agggcaagcc ctaacgaccc aaaagcaaat
     2161 ctagacccaa taaacaacct catatagccg taaaaagcta tataaggctc atataggcgt
     2221 tagacctcct tgccgataaa aaactaaggt ctgaaacgat aaaggcttag aacgcaaaat
     2281 atgccgtttt agagctatta tagaaaatgt ggttcgataa ttcacggaca caaaaagcta
     2341 tgtagtaagg acacatttcc taggtgcttg tgtctttatt ttttattgga tacttgctat
     2401 aatcaaagtg cgaaatgtga agattttgta caagagaaac acaaaagcca accctattgc
     2461 gagtagggtt ggcttttttt cgttcgttgc gacttcgaac
//
