LOCUS       NZ_CP117501             5185 bp    DNA     circular CON 05-APR-2024
DEFINITION  Enterococcus faecalis strain CQHZJ21-185 plasmid pHZJ21-5K, complete
            sequence.
ACCESSION   NZ_CP117501
VERSION     NZ_CP117501.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN33180039
            Assembly: GCF_036774845.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Tang,B. and Ni,J.
  TITLE     Complete genome sequence of Enterococcus faecalis CQHZJ21-185
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Tang,B. and Ni,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2023) Institute of Agro-product Safety and
            Nutrition, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao
            Road, Hangzhou, Zhejiang 310021, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. NOV-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_036774845.1-RS_2024_04_05
            Annotation Date                   :: 04/05/2024 15:48:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,945
            CDSs (total)                      :: 2,868
            Genes (coding)                    :: 2,819
            CDSs (with protein)               :: 2,819
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 16 of 49
            Pseudo Genes (incomplete)         :: 25 of 49
            Pseudo Genes (internal stop)      :: 12 of 49
            Pseudo Genes (multiple problems)  :: 4 of 49
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117501.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="CQHZJ21-185"
                     /isolation_source="feces"
                     /db_xref="taxon:1351"
                     /plasmid="pHZJ21-5K"
                     /geo_loc_name="China:Zhejiang"
                     /collection_date="2021-11"
     gene            join(5056..5185,1..167)
                     /locus_tag="PSC76_RS14700"
                     /old_locus_tag="PSC76_14670"
     CDS             join(5056..5185,1..167)
                     /locus_tag="PSC76_RS14700"
                     /old_locus_tag="PSC76_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002404941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002404941.1"
                     /translation="MGLMTGYNMTKEEYEITQAVLTRLDKFESELESLLLDLEPLDLSR
                     LDTVVREETLRMFNYLSRSYILLLDTVAYKDTLEEDISSGIVKIDGQPLDEEE"
                     /name="hypothetical protein"
     gene            946..2625
                     /gene="mobV"
                     /locus_tag="PSC76_RS14705"
                     /old_locus_tag="PSC76_14675"
     CDS             946..2625
                     /gene="mobV"
                     /locus_tag="PSC76_RS14705"
                     /old_locus_tag="PSC76_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401964.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_104659816.1"
                     /translation="MSKIVARMEKMKVGNLSGIQRHNQRETDNHSNPDIDIEKSHLNYD
                     LVNPGSINYREKIKQIIESQRISKRAVRKDAVLVNEWIITSDTAFFQENTDTQAFFTDV
                     VAYFSDRCGRQNVAYATVHLDETTPHMHLGIVPMYEGRLSSKQVFSRQSLLEIQEELPN
                     YLKERGYAIERGLRGSPQKHLSVKEYKDVQKEIQVSKQELANQEQILVEKERELHSYEQ
                     AIFRIDRLLEEKNEEVKGKVQAMNQLETKRNEIINSLQTTVLPDLANIKEKKFSFNGLK
                     YVLSASDLSAIKSITKDFETLRTQNKHLKIENNTLAQKNKELTQVNHEQELQITDLAYE
                     LTLKKEQLTAFEEKQLNYDQSEQIKQELDRTTQKVSVLETENKRLKDKITDLTYDFSEL
                     NEKYQEIKLKFDGLTTYLHERLGQAKETILFTIQQGVKYLQNAPKLKKSLERYSKDRTY
                     LIDEKGTLFLPPDTGSKGVTMSLNPHFYPLKAPFYNRETGCYEFVGEYMANGRSRTNTL
                     QLSEEQVLEKKAFTFEQSTDKKAWQQSKKYSRDMSKGRGLSR"
                     /name="mobV"
     gene            4197..4928
                     /locus_tag="PSC76_RS14710"
                     /old_locus_tag="PSC76_14685"
     CDS             4197..4928
                     /locus_tag="PSC76_RS14710"
                     /old_locus_tag="PSC76_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360724.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_025188181.1"
                     /translation="MNEKSELAVKYQNELNLVALKQFNAKEMNLFFALCARMKDKGLDS
                     IIFSFEELKELSDYKMTAIKAFTNDLDSLYSKMLQLTYRNEYDDDGSFRRFVLFTGFDV
                     NVKKQTVEVSINPKLEGILNGLTTEFSRFELSAFTAIRSTYAKTLFRLLMQYRSTGYYT
                     VSIDEFKEIMDIPKSYTQMGQIDQRVIKPAMKELNNYFENLELTKIKAKKGNKIAKLEF
                     TFTGLKTNKPSITMHDWVNGE"
                     /name="replication initiation protein"
     Typing          872..2622
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.918"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cacgattaga tactgtcgtc cgagaagaaa cgctaagaat gtttaattat ctaagtcggt
       61 cgtacattct acttcttgat acggtcgcct acaaagatac gctagaagaa gatatttcca
      121 gtgggatcgt gaaaattgac ggtcagcctc tagacgaaga agaataaaga ctaggtctga
      181 ttgttataaa aaagtattag atattcgggg tacactcgaa aaatatcaag attagtctac
      241 ttccattttg gggtaggctt tctttttgca agcagagcaa catggtacca tgttgcgata
      301 ttttgaatga cacctaccag tatttttggt aggtgtcatt caaatttttg gggttccccc
      361 aatccccact gttgttattt tttgtctttt gttcttgaaa tttacttaat tgtttcatgt
      421 gtttcggaag agcataaggg aaactgggtc ccacacaagg tgggacgcct tgcagtgcaa
      481 ggctttcccg tggggtacac tttcgttctt cgctagcact ttactagcag gtactttcag
      541 gtaaactagc acatttttag cactcactag caggtttcta gcacccaaac tagcagattc
      601 ctagcagtga ctagcaggtt tttagcacca aattagcaca ttactagcac cggctagcag
      661 aaagctagca cgttcactaa tttcaggcag tcgttattta aacggttttt tgacggaggg
      721 gaatttggag tttctgcctt agcagaaatg gaaagtttga ccgtaggcaa tcggctaagt
      781 gcgagattgg gaagcctaaa ggctgacctt ccgagtacta gccgacagcc ctcggcagag
      841 cgcaccttta cgaagtaaag tatagtgcgt tatactttac ttggaagtgc tgccgaaatc
      901 caaagacaaa gaacgccaca agaaaaaaaa ggggggggag cgctcatgtc aaaaattgtc
      961 gcacgtatgg agaaaatgaa agttggaaac ttaagcggta tccaacggca taatcaacga
     1021 gaaaccgata atcattccaa tcctgatatt gatattgaga aatctcactt gaattatgac
     1081 ttagtcaatc ctggttcaat caattatcgg gagaaaatca aacaaatcat tgagagccaa
     1141 cgaatcagta aacgagcggt tagaaaagac gcagtccttg tgaacgaatg gataatcact
     1201 agtgataccg cctttttcca agagaataca gacacacaag cattttttac cgatgttgtc
     1261 gcgtatttct ctgatcgctg cggtcgacaa aatgtcgcct atgccacggt acatttagac
     1321 gaaaccacgc cccatatgca cttaggaatt gtgcctatgt acgaagggcg attgagcagt
     1381 aaacaggtgt ttagtcggca aagtttgtta gagattcagg aagaactgcc aaactatttg
     1441 aaagaacggg gctacgctat tgagcgtgga ctccgtggga gtccgcaaaa gcatttatct
     1501 gtcaaagaat acaaagacgt tcaaaaagaa atacaggttt ctaaacaaga actcgcgaat
     1561 caggaacaaa ttctggtaga aaaagaaaga gaactccatt cctatgaaca ggcaatcttc
     1621 cgtattgacc gtcttcttga agagaagaac gaagaagtaa agggcaaagt ccaagccatg
     1681 aaccaactgg aaacgaagcg aaacgaaatc attaactctc tacaaacaac cgttttacct
     1741 gatttagcaa atatcaaaga aaagaaattt agttttaacg gtttaaaata cgttctaagc
     1801 gcttctgacc tttcggcaat aaaaagtatt acgaaagatt ttgaaacgct tagaacgcaa
     1861 aataagcacc ttaaaatcga aaacaatacc ttggcgcaga aaaataaaga attgacacaa
     1921 gtaaatcatg aacaagaatt acaaataacg gacttagcct atgaattaac cctgaaaaaa
     1981 gaacaattaa cagcgtttga agaaaaacaa ctaaactatg accagagcga acaaatcaaa
     2041 caagaacttg atagaacaac acaaaaagtt tctgtgttag aaacagagaa taagcggtta
     2101 aaagataaaa ttactgactt aacctacgac ttttctgaac taaacgaaaa atatcaagag
     2161 ataaaactaa agtttgacgg gttaaccact taccttcatg agagactagg acaggccaaa
     2221 gaaaccattt tatttaccat tcagcaagga gtgaagtacc ttcagaacgc gcctaaatta
     2281 aaaaaatccc tggagcgata ttccaaggac agaacctacc tcattgacga aaaagggaca
     2341 ttgtttttac caccagatac ggggtcaaaa ggggtaacaa tgtccttaaa cccacatttc
     2401 taccccctaa aagccccgtt ttacaacaga gaaacagggt gttatgagtt tgtcggcgag
     2461 tatatggcaa atggccgttc acgaactaat actttacagc tgtctgaaga acaagtgcta
     2521 gagaagaaag cttttacgtt tgaacagtcg acagataaaa aggcttggca acagagtaaa
     2581 aaatattcaa gagatatgtc aaaaggacga ggtttatctc gttaaaaata atttaattga
     2641 taaattattg acacataaaa acagttaaat taaaatatat taataaaaat taaaaaaaag
     2701 tgatgtaatc attatgaaaa agttgctagt tctaatttta ggtgtgtctt ttatctctgt
     2761 tgctttaagt tcaactacag aagcaagtgc tagagagtgg gattctatag agtgtcagcc
     2821 aggattttat tggaaaaaag gcgggtggac tgcaggaggc tatcataaag ccggtggatt
     2881 ttgtatccca atgcatacaa gatggacaaa atagttgcta aatttgaggt tattttatga
     2941 aaaaatatct aataaaaaat atattttata ttactattcc tttagtttta agctatataa
     3001 cttctttttt agttaatatt gatttgccca tattgataat tattttttac ggaattttat
     3061 tatttttttt aattccttca gaagtatatt tgggaagtac aatggattac aacgcgaaag
     3121 tagtgaatcc cacttacaga ccagaaaata aaagctttga agatagtcca aaaagtaaaa
     3181 tattgtctat cctcatagta ttactatgtt taatactaac tatttcgata tggtatttct
     3241 ctaattgacg ttttgcgtaa tctaatctga cgtttgaaaa tacagaaaaa aaatgttatt
     3301 gtgtaagtgc gaagcgaaat taagcaaaat catgtttaca ccaaaaagcc ctcctattct
     3361 cagtaggagg gcttttttta cactgttgcg atttcagcgt tggagctaga aaagcaactg
     3421 catagattat ctgctaatcg tctagccaac gatcaactag caaaagtact aaacccacaa
     3481 aaagtggggc gataatcaac gaaattaagc tttcaatcat gtttacaccc cctttcgggg
     3541 cagttgccaa aacaagtata tcacaagaag aatttcttgt gggggttgtt agggcttgcc
     3601 ctgaccgcct gcaaggcgca atcttaacaa gattacctga aaccactaaa cacgtcttaa
     3661 agagcttcag acaagcaaat agagcttaac caaatgagtt accttgtcaa cggtattgat
     3721 tttgatttta gcgagtgaac gaagtgaacg ctgcaagcat aatgtgagcg aagaatttcg
     3781 ctcactcctt tttttgtttt tcaatttttt agtgacagtt tcgtaaatat cgcgaacctg
     3841 tttgtagaaa aaagagtgaa tttttaggcg ggaaatttcc tcgcacgcgc acgcgtttat
     3901 ttttatatat tttttatata ttttttaaaa cctttttagg cggtctgtgt gccacgtggc
     3961 tctaaggaag agcagagggt accaacatca ttttatctgt gtaccaacat cattttatct
     4021 gtgtaccaac atcattttat ctgtgtacca acatcatttt atctgtgtac caacatcatt
     4081 ttatctgact actaataaca aattatcttt gtaaaatcac gctcacatta tataaacagc
     4141 atacttgaca ttaatgtatg ctgtttatat aatgtgagcg tgagggggcg aaaggaatga
     4201 atgaaaaatc ggaattagct gttaaatatc aaaacgaatt aaatctagtt gctttaaaac
     4261 agttcaatgc taaagaaatg aacttatttt ttgccttatg tgcaagaatg aaagacaaag
     4321 gtttagacag tatcatattt tcttttgaag agttaaaaga attaagcgac tataaaatga
     4381 ctgcaattaa ggcgtttaca aatgatttag atagcctata cagtaaaatg ctgcaactaa
     4441 cttatcggaa tgagtatgat gatgacggaa gttttcgacg atttgtcttg tttactggct
     4501 ttgacgttaa tgtaaaaaaa caaacagttg aagtaagtat caatcctaaa ttggaaggta
     4561 ttcttaatgg cttaacaact gaatttagta ggttcgagtt atctgcattt actgctatcc
     4621 gatcaaccta tgctaaaacg ttgtttcgct tattaatgca atatcggtca actggttact
     4681 ataccgttag tattgatgaa tttaaagaaa taatggatat tccaaaatcg tatactcaaa
     4741 tgggacaaat agaccaaaga gtgataaaac ctgcaatgaa agaactcaat aattactttg
     4801 agaatttaga attaacaaaa atcaaagcca aaaaaggcaa taagattgcc aaactagaat
     4861 ttacgtttac tggtctgaaa accaacaaac caagcataac tatgcatgac tgggtaaacg
     4921 gagaataaaa aaagaacttg ccccctcaca ttaagcaagt tagcaagttc ctcacgcaat
     4981 ccactacgac tgctagatta ttttagcata tttgcgtgag gacttctaaa ataaattatt
     5041 ttggaggtca tatacatggg attaatgaca ggatacaaca tgacaaaaga ggagtacgaa
     5101 atcacacagg ctgtactgac tcgactagat aagtttgaaa gtgaactaga atcattatta
     5161 cttgatttag agccactcga cctat
//
