LOCUS       NZ_CP117485             2789 bp    DNA     circular CON 25-MAR-2024
DEFINITION  Klebsiella pneumoniae strain DS-1 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP117485
VERSION     NZ_CP117485.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32958782
            Assembly: GCF_028621835.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   cao,d.
  TITLE     Aerobic biodegradation of phenanthrene by a newly isolated
            Klebsiella sp. DS-1 from wastewater
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   cao,d.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2023) School of Environment, Northeast Normal
            University, Jingyue Street, Changchun, Jilin 130117, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Fyle v. v2.5; CANU v. v1.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 253.0x
            Sequencing Technology             :: Illumina NovaSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028621835.1-RS_2024_03_25
            Annotation Date                   :: 03/25/2024 01:47:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,584
            CDSs (total)                      :: 5,455
            Genes (coding)                    :: 5,365
            CDSs (with protein)               :: 5,365
            Genes (RNA)                       :: 129
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 38 of 90
            Pseudo Genes (incomplete)         :: 46 of 90
            Pseudo Genes (internal stop)      :: 18 of 90
            Pseudo Genes (multiple problems)  :: 12 of 90
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117485.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="DS-1"
                     /isolation_source="wastewater"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="China:Jilin"
                     /collection_date="2022-08-20"
     gene            join(2596..2789,1..4)
                     /locus_tag="PPH92_RS27845"
                     /old_locus_tag="PPH92_27845"
     CDS             join(2596..2789,1..4)
                     /locus_tag="PPH92_RS27845"
                     /old_locus_tag="PPH92_27845"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045358392.1"
                     /translation="MERVLGIYVVGIIVSAGFLFIQEVRNGRRRSYTEEGASMLVGIIC
                     AFLWPLVLIAYFFDKKNGKL"
                     /name="hypothetical protein"
     gene            1..420
                     /locus_tag="PPH92_RS27850"
                     /old_locus_tag="PPH92_27850"
     CDS             1..420
                     /locus_tag="PPH92_RS27850"
                     /old_locus_tag="PPH92_27850"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128336926.1"
                     /translation="MKLSLFFISVITFLLAISPIAYSDNTIIPDRVYDPYRNSAVTPDG
                     TVYDRLTGGVLKASEHPQPNFGFPVQSSSDSCDTTYVKARTEDGRLLVTSDGVTLHVVD
                     NIVDVAHWQPNDELRVCGDRVINIDDNTSSSFTYN"
                     /name="hypothetical protein"
     gene            1247..1438
                     /locus_tag="PPH92_RS27855"
                     /old_locus_tag="PPH92_27855"
     CDS             1247..1438
                     /locus_tag="PPH92_RS27855"
                     /old_locus_tag="PPH92_27855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_045358396.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFCTLTARLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1438..1839
                     /locus_tag="PPH92_RS27860"
                     /old_locus_tag="PPH92_27860"
     CDS             1438..1839
                     /locus_tag="PPH92_RS27860"
                     /old_locus_tag="PPH92_27860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_024623230.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(515..1024)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.038"
                     /coverage="99.0"
                     /evalue="1.71e-152"
ORIGIN
        1 atgaagttaa gtctgttttt catctcggtc attacttttt tattggctat atcaccgata
       61 gcatactcag ataacacgat tattcctgac agagtatatg atccttatag aaattcggct
      121 gtaactcctg atggcaccgt ttatgaccgt ctgacaggag gagttttgaa ggcatcagag
      181 caccctcaac ctaattttgg gtttcctgtt caatcctcat cggatagttg tgatacaacg
      241 tatgttaaag ctaggacaga agacggtagg cttttagtca cgagcgacgg agtaactctg
      301 catgtagttg acaatattgt tgatgttgct cactggcaac caaatgatga gcttagagtc
      361 tgtggcgata gagtaatcaa tattgatgac aatacttcct cctcgttcac atacaattaa
      421 cctcttttat tcaaagaata tctatggtga tgatagaaag attgacgagc atgtatgggg
      481 ggatagccga agaaatgaac caaagttcaa aaggatcttc ttgagatcct ttcaggagcg
      541 tcgtaatctc ttgctctgaa aacgaaaaaa ccacctgggg aggtggtttt tcgaaggttc
      601 agtaagttgg ggaacttctg aaccgtggta acagggtgta caagaccgct gccaccaaat
      661 tcgtcctttc agtttagccg cagtctggct ctaacttcaa gaactttgca tcagtaatct
      721 cttgtacacc ctctaccaat ggctaccgcc aatggcgatt tgtcgtgcct tttcgggttg
      781 gactcaagac gatagttacc gaaaagagcg tggtagtcgg actgaacggg gggttcgtgc
      841 atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg tggaatgaga
      901 caaacgcggc cataacagcg gaatgacacc ggtaaaccga atggcaggaa caggagagcg
      961 cacgagggag ccatcagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1021 ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa
     1081 cggccgctgt gctgccttct ccctctccga gctgtctgct ctatgtcgtt atgtgcgcgt
     1141 tgcgaccgct cgccgcagcc gaacggcagg agcgtagcga ctgagtgagc gaggaagcgg
     1201 aaaagagatc tgtctgacat tgagcactga cgctctgagg ccctttatga cgaaacaaca
     1261 gaaaacagcg ctgaacatgg ctaaattcat ccagaatcag tctctgctcc tgctggagaa
     1321 gcttaacgag cttgatctcg atgtcgaggc cgacctttgt gagaaactcc acgatgatgc
     1381 ggagcatctt ttctgtacac tgaccgcgag actggatgca ttacaggatg gtaactgatg
     1441 gccagagtgt ctattagtga ggctgcccga ctgacgggta agagtagaac aacactgcac
     1501 aggttaatta aagcaggtga cctgtccacc tgtagcggtg aacgtaatgc aaaaatggta
     1561 gatacttcag agcttttacg tgtctttggc ccctttgaac aactgaaggg tgaacaccat
     1621 tctggacagg tctctgaaca gcgtgataca gcctctgctg accagagtga acaggtgatt
     1681 cgtcatctga aacaggagat tgaacacctg aacaccttac ttttgtccaa ggattcgcac
     1741 attgacagcc tgaaacaggc catgctcctt cttgaaaatc accaggcaaa attaactgaa
     1801 tccccggcgc catggtggaa attctggaaa aaatcctgac cccgtcatgc ttcagttttc
     1861 ctctccggcc atcccaggga gttcctgaac cttcccaaaa atcgtcagat ctctgaacgc
     1921 tcctcaccac taaaaatacc cacaccccac gcaaaattaa aattttgcag attccttatt
     1981 tacaacaatg ggttaatgaa aaataaatgt gcggtgatgt gttacaaagt gcagatccaa
     2041 gtcgtggacg actctatggc gaatcgctga cgaattgccc acgattcata tgtgaatcac
     2101 gctttattat catattgtta tgatgaatcg actcttgatt caattcatca ctttgggtgt
     2161 tttttgctca tggtgactct tgattcaatt cacgattcga atcgccggtg aatcgtgtgg
     2221 tgagtcgtcc ggcgacttgc cataacgtga ttgccggccg tcgacgaacc cgattcctga
     2281 acgtgctgaa aaccggtttt ccggctgcgc cggcaacgct tcacggccac gcaataaatt
     2341 gcgtccccgt tcaccgtttt cggatggaaa gaacgccgga aacaggactt actctggctg
     2401 aatggcgtga attttcgcgc tatgcacttg cgtgcatact catgcatgcc ttaaaaacag
     2461 agcctgcgcg tttctggcgg gttttcgggt ggtttgttgc ctgttttacc ggtttcccct
     2521 cagaaacgcc ctgagaccgt tttagcggtg cgtacaaggg atgttatgtt taatctgata
     2581 agaacggaat gttttatgga aagggtatta ggaatttatg ttgtcggtat tattgtgagt
     2641 gccggtttcc tgtttatcca agaagttagg aacgggaggc gcagaagtta caccgaagaa
     2701 ggtgcgtcaa tgcttgtcgg cataatttgt gcgtttttgt ggcctttagt tttaattgca
     2761 tatttttttg ataaaaaaaa tgggaaatt
//
