LOCUS       NZ_CP117323            11944 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Salmonella enterica subsp. enterica serovar Derby strain RM001
            plasmid pRM001_1, complete sequence.
ACCESSION   NZ_CP117323
VERSION     NZ_CP117323.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32950806
            Assembly: GCF_028893035.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Derby
  ORGANISM  Salmonella enterica subsp. enterica serovar Derby
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 11944)
  AUTHORS   Lewis,G.L., Fenton,R.J., Moriyama,E.N., Loy,J.D. and Moxley,R.A.
  TITLE     Association of ISVsa3 with Multidrug Resistance in Salmonella
            enterica Isolates from Cattle (Bos taurus)
  JOURNAL   Microorganisms 11 (3), 631 (2023)
   PUBMED   36985205
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11944)
  AUTHORS   Moxley,R.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2023) School of Biological Sciences, University of
            Nebraska-Lincoln, 403 Manter Hall, Lincoln, NE 68588-0118, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 04-OCT-2021
            Assembly Method                   :: Raven v. 1.5.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 343X
            Sequencing Technology :: illumina MiSeq + Nanopore Gridion
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/25/2023 10:17:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,745
            CDSs (total)                      :: 4,627
            Genes (coding)                    :: 4,490
            CDSs (with protein)               :: 4,490
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 65 of 137
            Pseudo Genes (incomplete)         :: 87 of 137
            Pseudo Genes (internal stop)      :: 19 of 137
            Pseudo Genes (multiple problems)  :: 31 of 137
            Pseudo Genes (short protein)      :: 1 of 137
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117323.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11944
                     /organism="Salmonella enterica subsp. enterica serovar
                     Derby"
                     /mol_type="genomic DNA"
                     /strain="RM001"
                     /serovar="Derby"
                     /isolation_source="Manila ropes placed above water tanks
                     and feed bunks used as sampling devices"
                     /host="Bos taurus"
                     /sub_species="enterica"
                     /db_xref="taxon:28144"
                     /plasmid="pRM001_1"
                     /geo_loc_name="USA: midwestern"
                     /collection_date="2000-09-19"
                     /collected_by="University of Nebraska-Lincoln"
     gene            complement(75..890)
                     /gene="sul2"
                     /locus_tag="PQP82_RS23580"
                     /old_locus_tag="PQP82_23580"
     ARG             complement(79..890)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(1970..2149)
                     /locus_tag="PQP82_RS23585"
                     /old_locus_tag="PQP82_23585"
     CDS             complement(1970..2149)
                     /locus_tag="PQP82_RS23585"
                     /old_locus_tag="PQP82_23585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_274900955.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DESGVYTGLIIWHQ"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2217..2540
                     /locus_tag="PQP82_RS23590"
                     /old_locus_tag="PQP82_23590"
     CDS             2217..2540
                     /locus_tag="PQP82_RS23590"
                     /old_locus_tag="PQP82_23590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2749..>3186
                     /locus_tag="PQP82_RS23595"
                     /old_locus_tag="PQP82_23595"
                     /pseudo=""
     CDS             2749..>3186
                     /locus_tag="PQP82_RS23595"
                     /old_locus_tag="PQP82_23595"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3218..3778
                     /locus_tag="PQP82_RS23600"
                     /old_locus_tag="PQP82_23600"
     CDS             3218..3778
                     /locus_tag="PQP82_RS23600"
                     /old_locus_tag="PQP82_23600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            3785..4018
                     /locus_tag="PQP82_RS23605"
                     /old_locus_tag="PQP82_23605"
     CDS             3785..4018
                     /locus_tag="PQP82_RS23605"
                     /old_locus_tag="PQP82_23605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137427.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4502..4924)
                     /locus_tag="PQP82_RS23610"
                     /old_locus_tag="PQP82_23610"
     CDS             complement(4502..4924)
                     /locus_tag="PQP82_RS23610"
                     /old_locus_tag="PQP82_23610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001255518.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            5046..5324
                     /locus_tag="PQP82_RS23615"
                     /old_locus_tag="PQP82_23615"
     CDS             5046..5324
                     /locus_tag="PQP82_RS23615"
                     /old_locus_tag="PQP82_23615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(5316..5606)
                     /locus_tag="PQP82_RS23620"
                     /old_locus_tag="PQP82_23620"
     CDS             complement(5316..5606)
                     /locus_tag="PQP82_RS23620"
                     /old_locus_tag="PQP82_23620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047032.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            5846..7492
                     /gene="cka"
                     /locus_tag="PQP82_RS23625"
                     /old_locus_tag="PQP82_23625"
     CDS             5846..7492
                     /gene="cka"
                     /locus_tag="PQP82_RS23625"
                     /old_locus_tag="PQP82_23625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_011264160.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            complement(7489..7779)
                     /gene="cki"
                     /locus_tag="PQP82_RS23630"
                     /old_locus_tag="PQP82_23630"
     CDS             complement(7489..7779)
                     /gene="cki"
                     /locus_tag="PQP82_RS23630"
                     /old_locus_tag="PQP82_23630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            7809..7940
                     /locus_tag="PQP82_RS23635"
                     /old_locus_tag="PQP82_23635"
     CDS             7809..7940
                     /locus_tag="PQP82_RS23635"
                     /old_locus_tag="PQP82_23635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(8946..9137)
                     /locus_tag="PQP82_RS23640"
                     /old_locus_tag="PQP82_23640"
     CDS             complement(8946..9137)
                     /locus_tag="PQP82_RS23640"
                     /old_locus_tag="PQP82_23640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            9205..9526
                     /locus_tag="PQP82_RS23645"
                     /old_locus_tag="PQP82_23645"
                     /pseudo=""
     CDS             9205..9526
                     /locus_tag="PQP82_RS23645"
                     /old_locus_tag="PQP82_23645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            9516..9830
                     /locus_tag="PQP82_RS23650"
                     /old_locus_tag="PQP82_23650"
     CDS             9516..9830
                     /locus_tag="PQP82_RS23650"
                     /old_locus_tag="PQP82_23650"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_274900956.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGARVLRGAPEEVRELID
                     ATPFAKKYTSGVLSFAEQTLPPGERERVMESFERVLMPGLEKISTASCGWSTRTRGVLS
                     "
                     /name="hypothetical protein"
     gene            9797..10078
                     /locus_tag="PQP82_RS23655"
                     /old_locus_tag="PQP82_23655"
     CDS             9797..10078
                     /locus_tag="PQP82_RS23655"
                     /old_locus_tag="PQP82_23655"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_274900954.1"
                     /translation="MEHQDKGRLELNFVIPNMELASGKRLQPYYDRADRPRINAWQTLV
                     NHHYGLHDPNAPENRRTLVTPIIICRKRNRRPQKRLRGDWRLFTMPER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            10205..10765
                     /locus_tag="PQP82_RS23660"
                     /old_locus_tag="PQP82_23660"
     CDS             10205..10765
                     /locus_tag="PQP82_RS23660"
                     /old_locus_tag="PQP82_23660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            10772..>10996
                     /locus_tag="PQP82_RS23665"
                     /old_locus_tag="PQP82_23665"
                     /pseudo=""
     CDS             10772..>10996
                     /locus_tag="PQP82_RS23665"
                     /old_locus_tag="PQP82_23665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137388.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /name="MbeD family mobilization/exclusion protein"
     gene            <10996..11454
                     /locus_tag="PQP82_RS23670"
                     /old_locus_tag="PQP82_23670"
                     /pseudo=""
     CDS             <10996..11454
                     /locus_tag="PQP82_RS23670"
                     /old_locus_tag="PQP82_23670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(<11690..11932)
                     /gene="glmM"
                     /locus_tag="PQP82_RS23675"
                     /old_locus_tag="PQP82_23675"
                     /pseudo=""
     CDS             complement(<11690..11932)
                     /gene="glmM"
                     /locus_tag="PQP82_RS23675"
                     /old_locus_tag="PQP82_23675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891390.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /name="glmM"
     Typing          8774..8861
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(1342..1942)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.508"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(8207..8763)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.633"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2531..4125
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.624"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          9098..9495
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="87.218"
                     /coverage="100.0"
                     /evalue="3.0800000000000004e-70"
     Typing          9517..9776
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="80.46"
                     /coverage="92.0"
                     /evalue="7.610000000000001e-71"
     Typing          9998..10998
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.701"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cctgtcgtcg ggtcgccctc aaaggggaca tgcctgctga accgcgaata tagagaaata
       61 tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa taccgccagc
      121 ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc tgcggcggcg
      181 gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc tgtgagcgcg
      241 cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg cagccgcaat
      301 tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag aaaaaacccc
      361 atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag cgccgcgatg
      421 cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc ctcgcgccga
      481 tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga tttcgccaat
      541 tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc cacaccacgc
      601 gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg aatgccatct
      661 gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga cgaaacaggc
      721 gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc ctcggccatc
      781 agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc atcggagaaa
      841 ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat gggggcttct
      901 ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag ggaatcccca
      961 ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc attttggaaa
     1021 taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt tggtgctgca
     1081 agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa cagtgactgg
     1141 tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga gatccttttc
     1201 aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc ggtttgttcg
     1261 aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag accgccatta
     1321 ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc tcttaatcta
     1381 tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc gtgtctttcc
     1441 gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg aacggggggt
     1501 tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt caggcgtgga
     1561 atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc aggaacagga
     1621 gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc tgtcgggttt
     1681 cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg gagcctatgg
     1741 aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg gtatcttcca
     1801 ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac gaccgagcgt
     1861 agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg ctgacgcgcc
     1921 ggtgctgcct ttttttcctg ccggcatggt gcacttttca tgacaatcct cactgatgcc
     1981 aaatgattaa gccagtatac actccgctct cgtcagcgtg gtcgtgaagc gcttcgcaga
     2041 tgtcagcctg gtcgtcggcg tccagttcat tcagtttttc cagcagggtc agcgtctggc
     2101 ttctgatgaa gcgggccatg ttaagggcgg ttttttcctg tttagtcaca gttctgtttc
     2161 tccatgtgtg gggagtctgt cggaggtttt taatgatacc ggtgaaacgc gaaaggatgc
     2221 tcacgatacg ggtcacggat gaggaacatg cgcggctgct ggcacgttgt gagggaaaac
     2281 aacttgcgtc atggatgcgt aaggtctgcc tgggggcacc gccatcgaaa acgtcaggtc
     2341 ttccgacact ggcaccgccg ctgttgcgcc agttcgcttc ggtcggcaat aacctgaatc
     2401 agatagcccg gaagataaac agcggacagt ggtcggggca tgaccgggtt catgtggtgg
     2461 ctgcactgat ggccatcgga cgtgaactga gtgagctgag ggatgaggtc cgtaaacagg
     2521 gagaacggga tgatagttaa gtttcatgcc aggggaaaag gcggtggcag tggtccggtt
     2581 gattacctgc tgggccggga gcgtaaccgg gaaggggcga gagttctgcg gggtgctccc
     2641 gaagaggtgc gggaactcat cgatgccacg ccgtttgcga aaaaatacac ctcgggggtt
     2701 ctgtcttttg cagagcagac cctgccaccg ggagagcgtg aaagggtgat ggagagcttt
     2761 gagcgggtac tgatgcccgg tcttgaaaaa aatcagtaca gcatcctgtg ggtggagcac
     2821 caggacaagg ggcgtcttga gctgaatttt gtcatcccga atatggagct ggcgagcgga
     2881 aaacggctcc agccctatta cgaccgagca gaccgtccgc gaatcaatgc ctggcagaca
     2941 ctggtgaatc accattacgg gctgcacgac ccgaacgcac cggagaacag gcggacactt
     3001 gtcaccccga ataatctgcc gaaagcgaaa caggaggccg cagaagcgat tacgagggga
     3061 ctggaggctc tttaccatgc cggagcgcta aaaacgcgtc aggatgtcac tgaggcgctt
     3121 acagcggcag gttttgaggt tgtgaggaca accaggagca gtatcagtat cgccgacccg
     3181 gagggtgggc gtaatctcag gctgaaaggg gcgctgtatg agcaatcttt tacaaatggg
     3241 gacagacttc gagaagaaac tgaaagagcg tgccgtctct acagagaaca tgctgaacag
     3301 cgagttcagg aagctcgaag cgtctgtaga cagggaactg aacttaaacg ggcagaaaat
     3361 cagagacgct atcagcgcac acacagcagc ggtgaaagaa caactggaga cgctgaacag
     3421 tgccgtgaac gcgcagttca gcgccacaga gaacgaactg gcccggcggc aggaagagct
     3481 gctctggaag ctggtcaaag ggcggatact gtacccgtcg ctgacggcgc tttgcgtcac
     3541 gggcgggatt tttctggcgt catgggggct gattcagtgg caggaaagcc ggattgcggc
     3601 gaacatcctg gctatcaggg accaggaaga gacgctggcg aaactccggg aaaaaacatg
     3661 gggtgtgacg tatcacgagg gcaggaacgg gaaatttctg gtgttgccgt ccggggtgaa
     3721 gggggaaaac aactggacgg tggagaagaa gaacgcagta agactggtca gggagtaagt
     3781 gagtatgaca gagctggaaa tgcacttact gaacgcctta gagcaattgc agcaggacta
     3841 tacgcagcgg ctgaaagact gggagagcgc cttcacggat ttgcagcata tgttcgagct
     3901 gacacgacag gagaacgggc aactgaggga acagtgcgac gacttgagtc agcaggtgca
     3961 gagattagcc gggcaggtgg accacttgag tcggttattt atcacgaaca gcagataagg
     4021 gatgaacagg aaaggcaggc acgcctggaa cgggagcgtg cgcggtcctg gcatgaagag
     4081 caggaacggg aagcacgggt atggcgtggc ccgtcaatag ggatgtgacg gtgagttgtt
     4141 ccgggaggtt gccagatggt accggaacgc gttaaaaacg catccgggcg cgattctggc
     4201 gtgtcgtttt tgttgctgtg ttttgcctct ttgccagatc gcgctcattc ctcagcctca
     4261 tcccgcgatg tctgcgtatg tcatcaactg aaaagatgga aaaaaaacag tgtcatgagg
     4321 tttacgcagg caaaactagc gttattcaca tggctgaata gctatgcata cggttaaaat
     4381 ttatcaggtg cgatcgcggc agcttttcgg gtggtttgtt gccattttta cctgtcagca
     4441 gccgtgatcg cgctgaacgc atttcagcgg tgcgtacaat taagggatta tggtaaatcc
     4501 cttactgtct gccctcgtat ccatcgagat aaaccgcaga aatggtgtct gccattagtt
     4561 tcattttttt cggcgaaggc atatcgctgt ttgtgtattt tctgccagca aaggctgttg
     4621 tgctccggag tgagtgaaaa aatttttctg agtggaattc ttttacacgt tgctgtgccg
     4681 tttctgctga tactccggct gctttgtctt tcatccccat atcccggaac tgctgaaaga
     4741 acgggagcat catacgtatg gtgtctgctt tctgggtgta gaagttagga tcaaatccat
     4801 catctgaagc ataaacataa tgccgggtat gtcgttccag gctggtcttt ggtgtgcatc
     4861 ctgtaagagc tattgttgct gttatcagag gtatcagaag cagcctggca gcggcagatc
     4921 tcataaacat ctccttgcgt ttttaatctt gatactcagg gagttatatc tccgaagagt
     4981 atctcttatc atacgtcggt ttactcccaa tgtgatttga tggtcaatga gtcaggaata
     5041 atcccgtgaa aaaatgtgtg ccagtcctgc tttttagtgt tctttctgct tcggttgcca
     5101 gtcatgcgga cagcaatgtt tttcaacaaa aggccgagaa gacaaaaaac ggcattgttg
     5161 cggatgttaa tgccagacag aaaatgttca gtaacagact gaagatacaa cttcagatcg
     5221 atgagttgag agcagagctg gctggcacca gagatgccca aaaaagagag gatttgcgta
     5281 aaaaactgga gtctctggag cggcaaaaga aatccttacg ctgatggggg cgtattttcc
     5341 ccggtttctt ccataacctt tttaccgttc agatatttac gggcgaggcg aatgcgtctt
     5401 gcctggtata ccgccgagta agcctgggtg ggattcacac cattactgat ttcactgacc
     5461 acatgacgct gatcagccag tgtcagcgtg taaacctgtt tctggatgag gtcacgggct
     5521 ttgtcatgct catatccggc agcaacaagg gtgtcgagaa gtcgtttgtc tgttttgttc
     5581 agtttatggc ggggtgattt gctcacatcg ttctccgttt tttcatgatc caacagtttt
     5641 atatctcatc ctgatttgct ccggtgttct gtttcgacgt tctcaatgcc tgcctcccca
     5701 tagtgtgatc cttattgcaa aaatgaaaat ccatgctctt gacatggaca atgctgagta
     5761 gtaggtttta ctgtatataa aaccagtggt tatatataca gtatattgtt tttaatttat
     5821 tgttttaatt atcaaagagg aatttatggc taaagaacta agtggatatg gaccaactgc
     5881 tggtgagtct atggggggaa caggggcgaa tctgaatcag caaggtggaa ataataatag
     5941 taatagtggg gttcactggg gtggtggctc tgggcatggt aataatggtg gtcagggaaa
     6001 ttcaaattca tctggttcta catcaactgt gatgaaaacc ggtgagtcat atctgactcc
     6061 gtggggtgat gttgtcatta ataatgatgg ccttccggtg atgaatggta ttgttatgac
     6121 agaagagaat tcaactctgg ttgataatcc atttggaggg gtctccagag ttcttaattc
     6181 tttaattagt gatatgccat cactttttgc agaaagctca ggaaataaca acaataacac
     6241 tgcttcagtt aatactgcac caacgaatgc tcaggtcagt gatatggata agagttcgaa
     6301 ggtggtgggt aatgtaatta atgaaaaaca aaagcagaag aataaaattg ctacacagat
     6361 tagtgaaaaa cagaagaaaa tcgaggagat gaaaaaagta ttcaagcatc attcttatca
     6421 tggtatcact gatcttgaga gggatgttga tgagttgcaa aaaaaatcaa atcagcttga
     6481 tgcagatatt agcaaactta attcttataa aaatacattg caaagtaaaa ttggtgatgt
     6541 gaataaacag aaggaagctg aagaaaaggc tcgtgaaaat gcagaggtcg ctgaacatga
     6601 aactcttaat gaagagaaac aggcggtggc ggaagcagag aagcgtttag ctgaggcgaa
     6661 agctgaactg gcgaaggctg aaagtgatgt acagagtaag caagctactg tttccagagt
     6721 tgcaggagag cttgaaaacg ctcagaagag tgttgacgtg aaggtcaccg gatttccggg
     6781 atggcgtgac gttcagaaaa aactacagcg tcagcttgaa gctaagcagg ctgagtactc
     6841 cgcagttgaa aatgaactta agaatgctgt tagtttcagg gatggtaagg ccgctgaggt
     6901 taaagaggca gagcagaaat taaaagaagc tcaggatgct cttgaaaaga gccagataaa
     6961 agatgctgtt gatactatgg ttggatttta tcaatatata accgaacaat atggagaaaa
     7021 atacgcaaaa attgctcagg atttagcgga aaagtctaaa ggaaagaaaa ttcaaggtgt
     7081 tgatgaagct ctggcagcat ttgaaaagta taagaatgta ctggataaga aattcagtaa
     7141 ggttgatcgg gatgctattt ttaatgcgtt agagtctgtt aattatgatg agttatctaa
     7201 gaatttaacg aagatatcaa agtcgcttaa aatcaccagt cgagtatctt ttctatatga
     7261 tgttggttct gattttaaaa atgcaataga aactggtaac tggcgacctt tgtttgtcac
     7321 gctggagaaa agtgcggtcg atgtaggtgt ggcaaaaatt gtggcattaa tgtttagttt
     7381 tattgttggt gtgcctcttg gtttctgggg gattgcaatt gtcacaggta ttgtttcttc
     7441 ttacataggg gatgatgagt tgagcaaact taatgagttg ttagggattt aatttctctt
     7501 tagagtgtaa tatatgaaac ttggcaatcc tactgggatt gccattaata aataaaacaa
     7561 tgcagataac ttgcataagt accagggttc tttttttaac tttgtatttt ctttgatata
     7621 tctagatatg gttaatttag agtatggata tagcaataca tgtattgatg atataaatat
     7681 aaataacaaa ggagtgttat cattgtcgtt aaatattaaa ataagaatcc atggtataag
     7741 tgattcaggt aaattatgta ggtagtattt taaatgcata agtattttta cttttgttgg
     7801 aaagaaatat gaaaataata atttttctaa ttacaatatt attcttgtca ggatgtcaag
     7861 caaactatat tcgtgatgta caaggaggaa ccgttgcacc atcttcgtct tctgaactga
     7921 cggggatcgc ggttcagtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7981 cgtaatctgc tgcctgtaaa cgaaaaaacc gcctggggag gcggtttagt cgaaggttaa
     8041 gtcagttggg gactgcttaa ccgggtaact ggctttagtg gagcacagat accaaatgct
     8101 gtccttccag tgtagccgct ataaggcttc aacttcaaga gttctgcgta tctaaatcca
     8161 ctatccagtt accaatggct gctgccagtg gcgttaagtc gtgtcttacc gggttggact
     8221 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8281 agcccagctt ggagcgaacg acctacaccg aactgagata ccaacagcgt gagctatgag
     8341 aaagcgccac gcttcccaaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8401 gaacaggaga gcgcacgagg gagccaccag ggggaaacgc ctggtatctt taagtcctgt
     8461 cgggtttcgc cacctctgac ttgagcgtcg gtttttgtga tgctcgtcag gggggcggag
     8521 cctatggaaa aacgccagct gcgtggcctt ttccgggttc ttgttttttg ctcacatgtt
     8581 tcttttggtg ttatcccctg attctgtgga taaccgcatt accgtctttg agtgagctga
     8641 taccgctcgc cgcagccgaa cgaccgagcg gagcgagtca gtgagcgagg aagcggaaaa
     8701 gcgcctggac gagcattttc tccttacgca tctgtgcggc atttcacacc cggcatggtg
     8761 cacttttcat acaatcctca ctgatgccaa atgattaagc cagtatacac tccgctatcg
     8821 ctacgtgact gggtcatggc tgcgccccga cacccgctaa aacccgctga cgcgccctga
     8881 cgggcttgtc tgctcccggc atccgctcac aggccagctg tgaccgtctc cgggagctgc
     8941 atgtgtcaga ggttttcacc gtcatccccg aagcgtgcga ggcagctgcg gtaaagctcg
     9001 tcagcgtggt cgtgaagcgc ttcgcagatg tcagcctggt cgtcggcgtc cagttcattc
     9061 agtttttcca gcagggtcag cgtctggctt ctgatgaagc gggccatgtt aagggcggtt
     9121 ttttcctgtt tagtcacagt tctgtttctc catgtgtggg gagtctgtcg gaggttttta
     9181 atgataccgg tgaaacgcga aaggatgctc acgatacggg tcacggatga ggaacatgcg
     9241 cggctgctgg cacgttgtga gggaaaacaa cttgcgtcat ggatgcgtaa ggtctgcctg
     9301 ggggcaccgc catcgaaaac gtcaggtctt ccgacactgg caccgccgct gttgcgccag
     9361 ttcgcttcgg tcggcaataa cctgaatcag atagcccgga agataaacag cggacagtgg
     9421 tcggggcatg accgggttca tgtggtggct gcactgatgg ccatcggacg tgaactgagt
     9481 gagctgaggg atgatccgta aacagggaga acgggatgat agttaagttt catgccaggg
     9541 gaaaaggcgg tggcagtggt ccggttgatt acctgctggg ccgggagcgt aaccgggaag
     9601 gggcgagagt tctgcggggt gctcccgaag aggtgcggga actcatcgat gccacgccgt
     9661 ttgcgaaaaa atacacctcg ggggttctgt cttttgcaga gcagaccctg ccaccgggag
     9721 agcgtgaaag ggtgatggag agctttgagc gggtactgat gcccggtctt gaaaaaatca
     9781 gtacagcatc ctgtgggtgg agcaccagga caaggggcgt cttgagctga attttgtcat
     9841 cccgaatatg gagctggcga gcggaaaacg gctccagccc tattacgacc gagcagaccg
     9901 tccgcgaatc aatgcctggc agacactggt gaatcaccat tacgggctgc acgacccgaa
     9961 cgcaccggag aacaggcgga cacttgtcac cccaataata atctgccgaa agcgaaacag
    10021 gaggccgcag aagcgattac gaggggactg gaggctcttt accatgccgg agcgctaaaa
    10081 acgcgtcagg atgtcactga ggcgcttaca gcggcaggtt ttgaggttgt gaggacaacc
    10141 aggagcagta tcagtatcgc cgacccggag ggtgggcgta atctcaggct gaaaggggcg
    10201 ctgtatgagc aatcttttac aaatggggac agacttcgag aagaaactga aagagcgtgc
    10261 cgtctctaca gagaacatgc tgaacagcga gttcaggaag ctcgaagcgt ctgtagacag
    10321 ggaactgaac ttaaacgggc agaaaatcag agacgctatc agcgcacaca cagcagcggt
    10381 gaaagaacaa ctggagacgc tgaacagtgc cgtgaacgcg cagttcagcg ccacagagaa
    10441 cgaactggcc cggcggcagg aagagctgct ctggaagctg gtcaaagggc ggatactgta
    10501 cccgtcgctg acggcgcttt gcgtcacggg cgggattttt ctggcgtcat gggggctgat
    10561 tcagtggcag gaaagccgga ttgcggcgaa catcctggct atcagggacc aggaagagac
    10621 gctggcgaaa ctccgggaaa aaacatgggg tgtgacgtat cacgagggca ggaacgggaa
    10681 atttctggtg ttgccgtccg gggtgaaggg ggaaaacaac tggacggtgg agaagaagaa
    10741 cgcagtaaga ctggtcaggg agtaagtgag tatgacagag ctggaaatgc acttactgaa
    10801 cgccttagag caattgcagc aggactatac gcagcggctg aaagactggg agagcgcctt
    10861 cacggatttg cagcatatgt tcgagctgac acgacaggag aacgggcaac tgagggaaca
    10921 gtgcgacgac ttgagtcagc aggtgcagag attagccggg caggtggacc acttgagtcg
    10981 gttatttatc acgaacgcta ccagctcaag acgccgtacc gggacggcac cacgcacgtc
    11041 attttcgaac cattggattt cattgcaagg ctggccgccc tggtaccgaa gcccagagtc
    11101 aacctaaccc gcttccacgg ggtgttcgca cccaacagtc ggcaccgggc gttggtcacg
    11161 ccggcaaaac ggggcagggg caacaaggtc agggtggctg atgaaccggc aacaccagca
    11221 caacggcgag cgtcgatgac atgggcgcaa cggctcaagc gtgttttcaa tatcgacatc
    11281 gagacctgca gcggctgcgg cggcgccatg aaagtcatcg cctgcattga agaccctata
    11341 gtgatcaagc agatccttga tcacctgaag cacaaagccg aaaccagcgg gaccagggcg
    11401 ttacccgaaa gccgggcgcc accggctgag ctgctcctgg gtctgtttga ctgacgagcc
    11461 tgaaggccaa cgataccaat caaaatgctg cgttcacagc gccgcggcag ggatccgccg
    11521 tgctggttgt cggaaaagga gccgctagtg ggaaagagga gggtaaattt tcagcgttgc
    11581 tggctccccg tcagccggat tgggttgcat cgcaggggtg tcgaaagagt caactgtggt
    11641 ccaaagctgt tggacttggg tgaaaagggc gtttattctt cctatacgtt gtcggcagcg
    11701 ggccaaaaag gaatacgtcc atgcccatcg aggtgaaacc ggctgtgagc gcgggttcaa
    11761 gcatatagcc cgacaggcgc gtatccttgc cgatcacgac acgatggcgg tggtcaccgc
    11821 gacgaaagac acggccagcc gccatgccga cgcgcaaggc ggtttccgcc gtcatcgcgc
    11881 cttcgttggc tttgccacga ataccgtctg tgccgaaata tttgcgcacc ataaggtcga
    11941 ttat
//
