LOCUS       NZ_CP117319             6988 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Salmonella enterica subsp. enterica serovar Derby strain RM002
            plasmid pRM002_2, complete sequence.
ACCESSION   NZ_CP117319
VERSION     NZ_CP117319.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32950807
            Assembly: GCF_028893015.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Derby
  ORGANISM  Salmonella enterica subsp. enterica serovar Derby
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Lewis,G.L., Fenton,R.J., Moriyama,E.N., Loy,J.D. and Moxley,R.A.
  TITLE     Association of ISVsa3 with Multidrug Resistance in Salmonella
            enterica Isolates from Cattle (Bos taurus)
  JOURNAL   Microorganisms 11 (3), 631 (2023)
   PUBMED   36985205
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6988)
  AUTHORS   Moxley,R.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2023) School of Biological Sciences, University of
            Nebraska-Lincoln, 403 Manter Hall, Lincoln, NE 68588-0118, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 04-OCT-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 510X
            Sequencing Technology :: illumina MiSeq + Nanopore Gridion
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/25/2023 10:33:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,807
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,517
            CDSs (with protein)               :: 4,517
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 172
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 172
            Pseudo Genes (frameshifted)       :: 83 of 172
            Pseudo Genes (incomplete)         :: 82 of 172
            Pseudo Genes (internal stop)      :: 40 of 172
            Pseudo Genes (multiple problems)  :: 30 of 172
            Pseudo Genes (short protein)      :: 1 of 172
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117319.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Salmonella enterica subsp. enterica serovar
                     Derby"
                     /mol_type="genomic DNA"
                     /strain="RM002"
                     /serovar="Derby"
                     /isolation_source="Water from tanks in pens"
                     /host="Bos taurus"
                     /sub_species="enterica"
                     /db_xref="taxon:28144"
                     /plasmid="pRM002_2"
                     /geo_loc_name="USA: midwestern"
                     /collection_date="2000-09-19"
                     /collected_by="University of Nebraska-Lincoln"
     gene            156..1802
                     /gene="cka"
                     /locus_tag="PQQ14_RS23950"
                     /old_locus_tag="PQQ14_23950"
     CDS             156..1802
                     /gene="cka"
                     /locus_tag="PQQ14_RS23950"
                     /old_locus_tag="PQQ14_23950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_011264160.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            complement(1799..2089)
                     /gene="cki"
                     /locus_tag="PQQ14_RS23955"
                     /old_locus_tag="PQQ14_23955"
     CDS             complement(1799..2089)
                     /gene="cki"
                     /locus_tag="PQQ14_RS23955"
                     /old_locus_tag="PQQ14_23955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            2119..2250
                     /locus_tag="PQQ14_RS23960"
                     /old_locus_tag="PQQ14_23960"
     CDS             2119..2250
                     /locus_tag="PQQ14_RS23960"
                     /old_locus_tag="PQQ14_23960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(3256..3447)
                     /locus_tag="PQQ14_RS23965"
                     /old_locus_tag="PQQ14_23965"
     CDS             complement(3256..3447)
                     /locus_tag="PQQ14_RS23965"
                     /old_locus_tag="PQQ14_23965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3515..3838
                     /locus_tag="PQQ14_RS23970"
                     /old_locus_tag="PQQ14_23970"
     CDS             3515..3838
                     /locus_tag="PQQ14_RS23970"
                     /old_locus_tag="PQQ14_23970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4047..>4484
                     /locus_tag="PQQ14_RS23975"
                     /old_locus_tag="PQQ14_23975"
                     /pseudo=""
     CDS             4047..>4484
                     /locus_tag="PQQ14_RS23975"
                     /old_locus_tag="PQQ14_23975"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4516..5076
                     /locus_tag="PQQ14_RS23980"
                     /old_locus_tag="PQQ14_23980"
     CDS             4516..5076
                     /locus_tag="PQQ14_RS23980"
                     /old_locus_tag="PQQ14_23980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            5083..5316
                     /locus_tag="PQQ14_RS23985"
                     /old_locus_tag="PQQ14_23985"
     CDS             5083..5316
                     /locus_tag="PQQ14_RS23985"
                     /old_locus_tag="PQQ14_23985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137427.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5800..6222)
                     /locus_tag="PQQ14_RS23990"
                     /old_locus_tag="PQQ14_23990"
     CDS             complement(5800..6222)
                     /locus_tag="PQQ14_RS23990"
                     /old_locus_tag="PQQ14_23990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001255518.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            6344..6622
                     /locus_tag="PQQ14_RS23995"
                     /old_locus_tag="PQQ14_23995"
     CDS             6344..6622
                     /locus_tag="PQQ14_RS23995"
                     /old_locus_tag="PQQ14_23995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(6614..6904)
                     /locus_tag="PQQ14_RS24000"
                     /old_locus_tag="PQQ14_24000"
     CDS             complement(6614..6904)
                     /locus_tag="PQQ14_RS24000"
                     /old_locus_tag="PQQ14_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047032.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     Typing          3084..3171
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(2517..3073)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.633"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3829..5423
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.624"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgcctcccca tagtgtgatc cttattgcaa aaatgaaaat ccatgctctt gacatggaca
       61 atgctgagta gtaggtttta ctgtatataa aaccagtggt tatatataca gtatattgtt
      121 tttaatttat tgttttaatt atcaaagagg aatttatggc taaagaacta agtggatatg
      181 gaccaactgc tggtgagtct atggggggaa caggggcgaa tctgaatcag caaggtggaa
      241 ataataatag taatagtggg gttcactggg gtggtggctc tgggcatggt aataatggtg
      301 gtcagggaaa ttcaaattca tctggttcta catcaactgt gatgaaaacc ggtgagtcat
      361 atctgactcc gtggggtgat gttgtcatta ataatgatgg ccttccggtg atgaatggta
      421 ttgttatgac agaagagaat tcaactctgg ttgataatcc atttggaggg gtctccagag
      481 ttcttaattc tttaattagt gatatgccat cactttttgc agaaagctca ggaaataaca
      541 acaataacac tgcttcagtt aatactgcac caacgaatgc tcaggtcagt gatatggata
      601 agagttcgaa ggtggtgggt aatgtaatta atgaaaaaca aaagcagaag aataaaattg
      661 ctacacagat tagtgaaaaa cagaagaaaa tcgaggagat gaaaaaagta ttcaagcatc
      721 attcttatca tggtatcact gatcttgaga gggatgttga tgagttgcaa aaaaaatcaa
      781 atcagcttga tgcagatatt agcaaactta attcttataa aaatacattg caaagtaaaa
      841 ttggtgatgt gaataaacag aaggaagctg aagaaaaggc tcgtgaaaat gcagaggtcg
      901 ctgaacatga aactcttaat gaagagaaac aggcggtggc ggaagcagag aagcgtttag
      961 ctgaggcgaa agctgaactg gcgaaggctg aaagtgatgt acagagtaag caagctactg
     1021 tttccagagt tgcaggagag cttgaaaacg ctcagaagag tgttgacgtg aaggtcaccg
     1081 gatttccggg atggcgtgac gttcagaaaa aactacagcg tcagcttgaa gctaagcagg
     1141 ctgagtactc cgcagttgaa aatgaactta agaatgctgt tagtttcagg gatggtaagg
     1201 ccgctgaggt taaagaggca gagcagaaat taaaagaagc tcaggatgct cttgaaaaga
     1261 gccagataaa agatgctgtt gatactatgg ttggatttta tcaatatata accgaacaat
     1321 atggagaaaa atacgcaaaa attgctcagg atttagcgga aaagtctaaa ggaaagaaaa
     1381 ttcaaggtgt tgatgaagct ctggcagcat ttgaaaagta taagaatgta ctggataaga
     1441 aattcagtaa ggttgatcgg gatgctattt ttaatgcgtt agagtctgtt aattatgatg
     1501 agttatctaa gaatttaacg aagatatcaa agtcgcttaa aatcaccagt cgagtatctt
     1561 ttctatatga tgttggttct gattttaaaa atgcaataga aactggtaac tggcgacctt
     1621 tgtttgtcac gctggagaaa agtgcggtcg atgtaggtgt ggcaaaaatt gtggcattaa
     1681 tgtttagttt tattgttggt gtgcctcttg gtttctgggg gattgcaatt gtcacaggta
     1741 ttgtttcttc ttacataggg gatgatgagt tgagcaaact taatgagttg ttagggattt
     1801 aatttctctt tagagtgtaa tatatgaaac ttggcaatcc tactgggatt gccattaata
     1861 aataaaacaa tgcagataac ttgcataagt accagggttc tttttttaac tttgtatttt
     1921 ctttgatata tctagatatg gttaatttag agtatggata tagcaataca tgtattgatg
     1981 atataaatat aaataacaaa ggagtgttat cattgtcgtt aaatattaaa ataagaatcc
     2041 atggtataag tgattcaggt aaattatgta ggtagtattt taaatgcata agtattttta
     2101 cttttgttgg aaagaaatat gaaaataata atttttctaa ttacaatatt attcttgtca
     2161 ggatgtcaag caaactatat tcgtgatgta caaggaggaa ccgttgcacc atcttcgtct
     2221 tctgaactga cggggatcgc ggttcagtag aaaagatcaa aggatcttct tgagatcctt
     2281 tttttctgcg cgtaatctgc tgcctgtaaa cgaaaaaacc gcctggggag gcggtttagt
     2341 cgaaggttaa gtcagttggg gactgcttaa ccgggtaact ggctttagtg gagcacagat
     2401 accaaatgct gtccttccag tgtagccgct ataaggcttc aacttcaaga gttctgcgta
     2461 tctaaatcca ctatccagtt accaatggct gctgccagtg gcgttaagtc gtgtcttacc
     2521 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2581 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata ccaacagcgt
     2641 gagctatgag aaagcgccac gcttcccaaa gggagaaagg cggacaggta tccggtaagc
     2701 ggcagggtcg gaacaggaga gcgcacgagg gagccaccag ggggaaacgc ctggtatctt
     2761 taagtcctgt cgggtttcgc cacctctgac ttgagcgtcg gtttttgtga tgctcgtcag
     2821 gggggcggag cctatggaaa aacgccagct gcgtggcctt ttccgggttc ttgttttttg
     2881 ctcacatgtt tcttttggtg ttatcccctg attctgtgga taaccgcatt accgtctttg
     2941 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg gagcgagtca gtgagcgagg
     3001 aagcggaaaa gcgcctggac gagcattttc tccttacgca tctgtgcggc atttcacacc
     3061 cggcatggtg cacttttcat acaatcctca ctgatgccaa atgattaagc cagtatacac
     3121 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgctaa aacccgctga
     3181 cgcgccctga cgggcttgtc tgctcccggc atccgctcac aggccagctg tgaccgtctc
     3241 cgggagctgc atgtgtcaga ggttttcacc gtcatccccg aagcgtgcga ggcagctgcg
     3301 gtaaagctcg tcagcgtggt cgtgaagcgc ttcgcagatg tcagcctggt cgtcggcgtc
     3361 cagttcattc agtttttcca gcagggtcag cgtctggctt ctgatgaagc gggccatgtt
     3421 aagggcggtt ttttcctgtt tagtcacagt tctgtttctc catgtgtggg gagtctgtcg
     3481 gaggttttta atgataccgg tgaaacgcga aaggatgctc acgatacggg tcacggatga
     3541 ggaacatgcg cggctgctgg cacgttgtga gggaaaacaa cttgcgtcat ggatgcgtaa
     3601 ggtctgcctg ggggcaccgc catcgaaaac gtcaggtctt ccgacactgg caccgccgct
     3661 gttgcgccag ttcgcttcgg tcggcaataa cctgaatcag atagcccgga agataaacag
     3721 cggacagtgg tcggggcatg accgggttca tgtggtggct gcactgatgg ccatcggacg
     3781 tgaactgagt gagctgaggg atgaggtccg taaacaggga gaacgggatg atagttaagt
     3841 ttcatgccag gggaaaaggc ggtggcagtg gtccggttga ttacctgctg ggccgggagc
     3901 gtaaccggga aggggcgaga gttctgcggg gtgctcccga agaggtgcgg gaactcatcg
     3961 atgccacgcc gtttgcgaaa aaatacacct cgggggttct gtcttttgca gagcagaccc
     4021 tgccaccggg agagcgtgaa agggtgatgg agagctttga gcgggtactg atgcccggtc
     4081 ttgaaaaaaa tcagtacagc atcctgtggg tggagcacca ggacaagggg cgtcttgagc
     4141 tgaattttgt catcccgaat atggagctgg cgagcggaaa acggctccag ccctattacg
     4201 accgagcaga ccgtccgcga atcaatgcct ggcagacact ggtgaatcac cattacgggc
     4261 tgcacgaccc gaacgcaccg gagaacaggc ggacacttgt caccccgaat aatctgccga
     4321 aagcgaaaca ggaggccgca gaagcgatta cgaggggact ggaggctctt taccatgccg
     4381 gagcgctaaa aacgcgtcag gatgtcactg aggcgcttac agcggcaggt tttgaggttg
     4441 tgaggacaac caggagcagt atcagtatcg ccgacccgga gggtgggcgt aatctcaggc
     4501 tgaaaggggc gctgtatgag caatctttta caaatgggga cagacttcga gaagaaactg
     4561 aaagagcgtg ccgtctctac agagaacatg ctgaacagcg agttcaggaa gctcgaagcg
     4621 tctgtagaca gggaactgaa cttaaacggg cagaaaatca gagacgctat cagcgcacac
     4681 acagcagcgg tgaaagaaca actggagacg ctgaacagtg ccgtgaacgc gcagttcagc
     4741 gccacagaga acgaactggc ccggcggcag gaagagctgc tctggaagct ggtcaaaggg
     4801 cggatactgt acccgtcgct gacggcgctt tgcgtcacgg gcgggatttt tctggcgtca
     4861 tgggggctga ttcagtggca ggaaagccgg attgcggcga acatcctggc tatcagggac
     4921 caggaagaga cgctggcgaa actccgggaa aaaacatggg gtgtgacgta tcacgagggc
     4981 aggaacggga aatttctggt gttgccgtcc ggggtgaagg gggaaaacaa ctggacggtg
     5041 gagaagaaga acgcagtaag actggtcagg gagtaagtga gtatgacaga gctggaaatg
     5101 cacttactga acgccttaga gcaattgcag caggactata cgcagcggct gaaagactgg
     5161 gagagcgcct tcacggattt gcagcatatg ttcgagctga cacgacagga gaacgggcaa
     5221 ctgagggaac agtgcgacga cttgagtcag caggtgcaga gattagccgg gcaggtggac
     5281 cacttgagtc ggttatttat cacgaacagc agataaggga tgaacaggaa aggcaggcac
     5341 gcctggaacg ggagcgtgcg cggtcctggc atgaagagca ggaacgggaa gcacgggtat
     5401 ggcgtggccc gtcaataggg atgtgacggt gagttgttcc gggaggttgc cagatggtac
     5461 cggaacgcgt taaaaacgca tccgggcgcg attctggcgt gtcgtttttg ttgctgtgtt
     5521 ttgcctcttt gccagatcgc gctcattcct cagcctcatc ccgcgatgtc tgcgtatgtc
     5581 atcaactgaa aagatggaaa aaaaacagtg tcatgaggtt tacgcaggca aaactagcgt
     5641 tattcacatg gctgaatagc tatgcatacg gttaaaattt atcaggtgcg atcgcggcag
     5701 cttttcgggt ggtttgttgc catttttacc tgtcagcagc cgtgatcgcg ctgaacgcat
     5761 ttcagcggtg cgtacaatta agggattatg gtaaatccct tactgtctgc cctcgtatcc
     5821 atcgagataa accgcagaaa tggtgtctgc cattagtttc atttttttcg gcgaaggcat
     5881 atcgctgttt gtgtattttc tgccagcaaa ggctgttgtg ctccggagtg agtgaaaaaa
     5941 tttttctgag tggaattctt ttacacgttg ctgtgccgtt tctgctgata ctccggctgc
     6001 tttgtctttc atccccatat cccggaactg ctgaaagaac gggagcatca tacgtatggt
     6061 gtctgctttc tgggtgtaga agttaggatc aaatccatca tctgaagcat aaacataatg
     6121 ccgggtatgt cgttccaggc tggtctttgg tgtgcatcct gtaagagcta ttgttgctgt
     6181 tatcagaggt atcagaagca gcctggcagc ggcagatctc ataaacatct ccttgcgttt
     6241 ttaatcttga tactcaggga gttatatctc cgaagagtat ctcttatcat acgtcggttt
     6301 actcccaatg tgatttgatg gtcaatgagt caggaataat cccgtgaaaa aatgtgtgcc
     6361 agtcctgctt tttagtgttc tttctgcttc ggttgccagt catgcggaca gcaatgtttt
     6421 tcaacaaaag gccgagaaga caaaaaacgg cattgttgcg gatgttaatg ccagacagaa
     6481 aatgttcagt aacagactga agatacaact tcagatcgat gagttgagag cagagctggc
     6541 tggaaccaga gatgcccaaa aaagagagga tttgcgtaaa aaactggagt ctctggagcg
     6601 gcaaaagaaa tccttacgct gatgggggcg tattttcccc ggtttcttcc ataacctttt
     6661 taccgttcag atatttacgg gcgaggcgaa tgcgtcttgc ctggtatacc gccgagtaag
     6721 cctgggtggg attcacacca ttactgattt cactgaccac atgacgctga tcagccagtg
     6781 tcagcgtgta aacctgtttc tggatgaggt cacgggcttt gtcatgctca tatccggcag
     6841 caacaagggt gtcgagaagt cgtttgtctg ttttgttcag tttatggcgg ggtgatttgc
     6901 tcacatcgtt ctccgttttt tcatgatcca acagttttat atctcatcct gatttgctcc
     6961 ggtgttctgt ttcgacgttc tcaatgcc
//
