LOCUS       NZ_CP117313             6988 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Salmonella enterica subsp. enterica serovar Derby strain RM003
            plasmid pRM003_1, complete sequence.
ACCESSION   NZ_CP117313
VERSION     NZ_CP117313.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32950808
            Assembly: GCF_028892995.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Derby
  ORGANISM  Salmonella enterica subsp. enterica serovar Derby
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Lewis,G.L., Fenton,R.J., Moriyama,E.N., Loy,J.D. and Moxley,R.A.
  TITLE     Association of ISVsa3 with Multidrug Resistance in Salmonella
            enterica Isolates from Cattle (Bos taurus)
  JOURNAL   Microorganisms 11 (3), 631 (2023)
   PUBMED   36985205
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6988)
  AUTHORS   Moxley,R.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2023) School of Biological Sciences, University of
            Nebraska-Lincoln, 403 Manter Hall, Lincoln, NE 68588-0118, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 04-OCT-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 624X
            Sequencing Technology :: illumina MiSeq + Nanopore Gridion
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/25/2023 10:14:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,719
            CDSs (total)                      :: 4,601
            Genes (coding)                    :: 4,418
            CDSs (with protein)               :: 4,418
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 88 of 183
            Pseudo Genes (incomplete)         :: 84 of 183
            Pseudo Genes (internal stop)      :: 45 of 183
            Pseudo Genes (multiple problems)  :: 31 of 183
            Pseudo Genes (short protein)      :: 1 of 183
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117313.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Salmonella enterica subsp. enterica serovar
                     Derby"
                     /mol_type="genomic DNA"
                     /strain="RM003"
                     /serovar="Derby"
                     /isolation_source="Manila ropes placed above water tanks
                     and feed bunks used as sampling devices"
                     /host="Bos taurus"
                     /sub_species="enterica"
                     /db_xref="taxon:28144"
                     /plasmid="pRM003_1"
                     /geo_loc_name="USA: midwestern"
                     /collection_date="2000-10-30"
                     /collected_by="University of Nebraska-Lincoln"
     gene            complement(join(6964..6988,1..167))
                     /locus_tag="PQP99_RS23470"
                     /old_locus_tag="PQP99_23470"
     CDS             complement(join(6964..6988,1..167))
                     /locus_tag="PQP99_RS23470"
                     /old_locus_tag="PQP99_23470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            235..558
                     /locus_tag="PQP99_RS23475"
                     /old_locus_tag="PQP99_23475"
     CDS             235..558
                     /locus_tag="PQP99_RS23475"
                     /old_locus_tag="PQP99_23475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            767..>1204
                     /locus_tag="PQP99_RS23480"
                     /old_locus_tag="PQP99_23480"
                     /pseudo=""
     CDS             767..>1204
                     /locus_tag="PQP99_RS23480"
                     /old_locus_tag="PQP99_23480"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1236..1796
                     /locus_tag="PQP99_RS23485"
                     /old_locus_tag="PQP99_23485"
     CDS             1236..1796
                     /locus_tag="PQP99_RS23485"
                     /old_locus_tag="PQP99_23485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            1803..2036
                     /locus_tag="PQP99_RS23490"
                     /old_locus_tag="PQP99_23490"
     CDS             1803..2036
                     /locus_tag="PQP99_RS23490"
                     /old_locus_tag="PQP99_23490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137427.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2520..2942)
                     /locus_tag="PQP99_RS23495"
                     /old_locus_tag="PQP99_23495"
     CDS             complement(2520..2942)
                     /locus_tag="PQP99_RS23495"
                     /old_locus_tag="PQP99_23495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_274880493.1"
                     /translation="MRSTAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            3064..3342
                     /locus_tag="PQP99_RS23500"
                     /old_locus_tag="PQP99_23500"
     CDS             3064..3342
                     /locus_tag="PQP99_RS23500"
                     /old_locus_tag="PQP99_23500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_274880494.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELASTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(3334..3624)
                     /locus_tag="PQP99_RS23505"
                     /old_locus_tag="PQP99_23505"
     CDS             complement(3334..3624)
                     /locus_tag="PQP99_RS23505"
                     /old_locus_tag="PQP99_23505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047032.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            3864..5510
                     /gene="cka"
                     /locus_tag="PQP99_RS23510"
                     /old_locus_tag="PQP99_23510"
     CDS             3864..5510
                     /gene="cka"
                     /locus_tag="PQP99_RS23510"
                     /old_locus_tag="PQP99_23510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_011264160.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            complement(5507..5797)
                     /gene="cki"
                     /locus_tag="PQP99_RS23515"
                     /old_locus_tag="PQP99_23515"
     CDS             complement(5507..5797)
                     /gene="cki"
                     /locus_tag="PQP99_RS23515"
                     /old_locus_tag="PQP99_23515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            5827..5958
                     /locus_tag="PQP99_RS23520"
                     /old_locus_tag="PQP99_23520"
     CDS             5827..5958
                     /locus_tag="PQP99_RS23520"
                     /old_locus_tag="PQP99_23520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     Typing          6792..6879
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(6225..6781)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.633"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          549..2143
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.624"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagcgtgcga ggcagctgcg gtaaagctcg tcagcgtggt cgtgaagcgc ttcgcagatg
       61 tcagcctggt cgtcggcgtc cagttcattc agtttttcca gcagggtcag cgtctggctt
      121 ctgatgaagc gggccatgtt aagggcggtt ttttcctgtt tagtcacagt tctgtttctc
      181 catgtgtggg gagtctgtcg gaggttttta atgataccgg tgaaacgcga aaggatgctc
      241 acgatacggg tcacggatga ggaacatgcg cggctgctgg cacgttgtga gggaaaacaa
      301 cttgcgtcat ggatgcgtaa ggtctgcctg ggggcaccgc catcgaaaac gtcaggtctt
      361 ccgacactgg caccgccgct gttgcgccag ttcgcttcgg tcggcaataa cctgaatcag
      421 atagcccgga agataaacag cggacagtgg tcggggcatg accgggttca tgtggtggct
      481 gcactgatgg ccatcggacg tgaactgagt gagctgaggg atgaggtccg taaacaggga
      541 gaacgggatg atagttaagt ttcatgccag gggaaaaggc ggtggcagtg gtccggttga
      601 ttacctgctg ggccgggagc gtaaccggga aggggcgaga gttctgcggg gtgctcccga
      661 agaggtgcgg gaactcatcg atgccacgcc gtttgcgaaa aaatacacct cgggggttct
      721 gtcttttgca gagcagaccc tgccaccggg agagcgtgaa agggtgatgg agagctttga
      781 gcgggtactg atgcccggtc ttgaaaaaaa tcagtacagc atcctgtggg tggagcacca
      841 ggacaagggg cgtcttgagc tgaattttgt catcccgaat atggagctgg cgagcggaaa
      901 acggctccag ccctattacg accgagcaga ccgtccgcga atcaatgcct ggcagacact
      961 ggtgaatcac cattacgggc tgcacgaccc gaacgcaccg gagaacaggc ggacacttgt
     1021 caccccgaat aatctgccga aagcgaaaca ggaggccgca gaagcgatta cgaggggact
     1081 ggaggctctt taccatgccg gagcgctaaa aacgcgtcag gatgtcactg aggcgcttac
     1141 agcggcaggt tttgaggttg tgaggacaac caggagcagt atcagtatcg ccgacccgga
     1201 gggtgggcgt aatctcaggc tgaaaggggc gctgtatgag caatctttta caaatgggga
     1261 cagacttcga gaagaaactg aaagagcgtg ccgtctctac agagaacatg ctgaacagcg
     1321 agttcaggaa gctcgaagcg tctgtagaca gggaactgaa cttaaacggg cagaaaatca
     1381 gagacgctat cagcgcacac acagcagcgg tgaaagaaca actggagacg ctgaacagtg
     1441 ccgtgaacgc gcagttcagc gccacagaga acgaactggc ccggcggcag gaagagctgc
     1501 tctggaagct ggtcaaaggg cggatactgt acccgtcgct gacggcgctt tgcgtcacgg
     1561 gcgggatttt tctggcgtca tgggggctga ttcagtggca ggaaagccgg attgcggcga
     1621 acatcctggc tatcagggac caggaagaga cgctggcgaa actccgggaa aaaacatggg
     1681 gtgtgacgta tcacgagggc aggaacggga aatttctggt gttgccgtcc ggggtgaagg
     1741 gggaaaacaa ctggacggtg gagaagaaga acgcagtaag actggtcagg gagtaagtga
     1801 gtatgacaga gctggaaatg cacttactga acgccttaga gcaattgcag caggactata
     1861 cgcagcggct gaaagactgg gagagcgcct tcacggattt gcagcatatg ttcgagctga
     1921 cacgacagga gaacgggcaa ctgagggaac agtgcgacga cttgagtcag caggtgcaga
     1981 gattagccgg gcaggtggac cacttgagtc ggttatttat cacgaacagc agataaggga
     2041 tgaacaggaa aggcaggcac gcctggaacg ggagcgtgcg cggtcctggc atgaagagca
     2101 ggaacgggaa gcacgggtat ggcgtggccc gtcaataggg atgtgacggt gagttgttcc
     2161 gggaggttgc cagatggtac cggaacgcgt taaaaacgca tccgggcgcg attctggcgt
     2221 gtcgtttttg ttgctgtgtt ttgcctcttt gccagatcgc gctcattcct cagcctcatc
     2281 ccgcgatgtc tgcgtatgtc atcaactgaa aagatggaaa aaaaacagtg tcatgaggtt
     2341 tacgcaggca aaactagcgt tattcacatg gctgaatagc tatgcatacg gttaaaattt
     2401 atcaggtgcg atcgcggcag cttttcgggt ggtttgttgc catttttacc tgtcagcagc
     2461 cgtgatcgcg ctgaacgcat ttcagcggtg cgtacaatta agggattatg gtaaatccct
     2521 tactgtctgc cctcgtatcc atcgagataa accgcagaaa tggtgtctgc cattagtttc
     2581 atttttttcg gcgaaggcat atcgctgttt gtgtattttc tgccagcaaa ggctgttgtg
     2641 ctccggagtg agtgaaaaaa tttttctgag tggaattctt ttacacgttg ctgtgccgtt
     2701 tctgctgata ctccggctgc tttgtctttc atccccatat cccggaactg ctgaaagaac
     2761 gggagcatca tacgtatggt gtctgctttc tgggtgtaga agttaggatc aaatccatca
     2821 tctgaagcat aaacataatg ccgggtatgt cgttccaggc tggtctttgg tgtgcatcct
     2881 gtaagagcta ttgttgctgt tatcagaggt atcagaagca gcctggcagc ggtagatctc
     2941 ataaacatct ccttgcgttt ttaatcttga tactcaggga gttatatctc cgaagagtat
     3001 ctcttatcat acgtcggttt actcccaatg tgatttgatg gtcaatgagt caggaataat
     3061 cccgtgaaaa aatgtgtgcc agtcctgctt tttagtgttc tttctgcttc ggttgccagt
     3121 catgcggaca gcaatgtttt tcaacaaaag gccgagaaga caaaaaacgg cattgttgcg
     3181 gatgttaatg ccagacagaa aatgttcagt aacagactga agatacaact tcagatcgat
     3241 gagttgagag cagagctggc tagcaccaga gatgcccaaa aaagagagga tttgcgtaaa
     3301 aaactggagt ctctggagcg gcaaaagaaa tccttacgct gatgggggcg tattttcccc
     3361 ggtttcttcc ataacctttt taccgttcag atatttacgg gcgaggcgaa tgcgtcttgc
     3421 ctggtatacc gccgagtaag cctgggtggg attcacacca ttactgattt cactgaccac
     3481 atgacgctga tcagccagtg tcagcgtgta aacctgtttc tggatgaggt cacgggcttt
     3541 gtcatgctca tatccggcag caacaagggt gtcgagaagt cgtttgtctg ttttgttcag
     3601 tttatggcgg ggtgatttgc tcacatcgtt ctccgttttt tcatgatcca acagttttat
     3661 atctcatcct gatttgctcc ggtgttctgt ttcgacgttc tcaatgcctg cctccccata
     3721 gtgtgatcct tattgcaaaa atgaaaatcc atgctcttga catggacaat gctgagtagt
     3781 aggttttact gtatataaaa ccagtggtta tatatacagt atattgtttt taatttattg
     3841 ttttaattat caaagaggaa tttatggcta aagaactaag tggatatgga ccaactgctg
     3901 gtgagtctat ggggggaaca ggggcgaatc tgaatcagca aggtggaaat aataatagta
     3961 atagtggggt tcactggggt ggtggctctg ggcatggtaa taatggtggt cagggaaatt
     4021 caaattcatc tggttctaca tcaactgtga tgaaaaccgg tgagtcatat ctgactccgt
     4081 ggggtgatgt tgtcattaat aatgatggcc ttccggtgat gaatggtatt gttatgacag
     4141 aagagaattc aactctggtt gataatccat ttggaggggt ctccagagtt cttaattctt
     4201 taattagtga tatgccatca ctttttgcag aaagctcagg aaataacaac aataacactg
     4261 cttcagttaa tactgcacca acgaatgctc aggtcagtga tatggataag agttcgaagg
     4321 tggtgggtaa tgtaattaat gaaaaacaaa agcagaagaa taaaattgct acacagatta
     4381 gtgaaaaaca gaagaaaatc gaggagatga aaaaagtatt caagcatcat tcttatcatg
     4441 gtatcactga tcttgagagg gatgttgatg agttgcaaaa aaaatcaaat cagcttgatg
     4501 cagatattag caaacttaat tcttataaaa atacattgca aagtaaaatt ggtgatgtga
     4561 ataaacagaa ggaagctgaa gaaaaggctc gtgaaaatgc agaggtcgct gaacatgaaa
     4621 ctcttaatga agagaaacag gcggtggcgg aagcagagaa gcgtttagct gaggcgaaag
     4681 ctgaactggc gaaggctgaa agtgatgtac agagtaagca agctactgtt tccagagttg
     4741 caggagagct tgaaaacgct cagaagagtg ttgacgtgaa ggtcaccgga tttccgggat
     4801 ggcgtgacgt tcagaaaaaa ctacagcgtc agcttgaagc taagcaggct gagtactccg
     4861 cagttgaaaa tgaacttaag aatgctgtta gtttcaggga tggtaaggcc gctgaggtta
     4921 aagaggcaga gcagaaatta aaagaagctc aggatgctct tgaaaagagc cagataaaag
     4981 atgctgttga tactatggtt ggattttatc aatatataac cgaacaatat ggagaaaaat
     5041 acgcaaaaat tgctcaggat ttagcggaaa agtctaaagg aaagaaaatt caaggtgttg
     5101 atgaagctct ggcagcattt gaaaagtata agaatgtact ggataagaaa ttcagtaagg
     5161 ttgatcggga tgctattttt aatgcgttag agtctgttaa ttatgatgag ttatctaaga
     5221 atttaacgaa gatatcaaag tcgcttaaaa tcaccagtcg agtatctttt ctatatgatg
     5281 ttggttctga ttttaaaaat gcaatagaaa ctggtaactg gcgacctttg tttgtcacgc
     5341 tggagaaaag tgcggtcgat gtaggtgtgg caaaaattgt ggcattaatg tttagtttta
     5401 ttgttggtgt gcctcttggt ttctggggga ttgcaattgt cacaggtatt gtttcttctt
     5461 acatagggga tgatgagttg agcaaactta atgagttgtt agggatttaa tttctcttta
     5521 gagtgtaata tatgaaactt ggcaatccta ctgggattgc cattaataaa taaaacaatg
     5581 cagataactt gcataagtac cagggttctt tttttaactt tgtattttct ttgatatatc
     5641 tagatatggt taatttagag tatggatata gcaatacatg tattgatgat ataaatataa
     5701 ataacaaagg agtgttatca ttgtcgttaa atattaaaat aagaatccat ggtataagtg
     5761 attcaggtaa attatgtagg tagtatttta aatgcataag tatttttact tttgttggaa
     5821 agaaatatga aaataataat ttttctaatt acaatattat tcttgtcagg atgtcaagca
     5881 aactatattc gtgatgtaca aggaggaacc gttgcaccat cttcgtcttc tgaactgacg
     5941 gggatcgcgg ttcagtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6001 taatctgctg cctgtaaacg aaaaaaccgc ctggggaggc ggtttagtcg aaggttaagt
     6061 cagttgggga ctgcttaacc gggtaactgg ctttagtgga gcacagatac caaatgctgt
     6121 ccttccagtg tagccgctat aaggcttcaa cttcaagagt tctgcgtatc taaatccact
     6181 atccagttac caatggctgc tgccagtggc gttaagtcgt gtcttaccgg gttggactca
     6241 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6301 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc aacagcgtga gctatgagaa
     6361 agcgccacgc ttcccaaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6421 acaggagagc gcacgaggga gccaccaggg ggaaacgcct ggtatcttta agtcctgtcg
     6481 ggtttcgcca cctctgactt gagcgtcggt ttttgtgatg ctcgtcaggg gggcggagcc
     6541 tatggaaaaa cgccagctgc gtggcctttt ccgggttctt gttttttgct cacatgtttc
     6601 ttttggtgtt atcccctgat tctgtggata accgcattac cgtctttgag tgagctgata
     6661 ccgctcgccg cagccgaacg accgagcgga gcgagtcagt gagcgaggaa gcggaaaagc
     6721 gcctggacga gcattttctc cttacgcatc tgtgcggcat ttcacacccg gcatggtgca
     6781 cttttcatac aatcctcact gatgccaaat gattaagcca gtatacactc cgctatcgct
     6841 acgtgactgg gtcatggctg cgccccgaca cccgctaaaa cccgctgacg cgccctgacg
     6901 ggcttgtctg ctcccggcat ccgctcacag gccagctgtg accgtctccg ggagctgcat
     6961 gtgtcagagg ttttcaccgt catccccg
//
