LOCUS       NZ_CP117308             6988 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Salmonella enterica subsp. enterica serovar Derby strain RM004
            plasmid pRM004_1, complete sequence.
ACCESSION   NZ_CP117308
VERSION     NZ_CP117308.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32950809
            Assembly: GCF_028892975.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Derby
  ORGANISM  Salmonella enterica subsp. enterica serovar Derby
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Lewis,G.L., Fenton,R.J., Moriyama,E.N., Loy,J.D. and Moxley,R.A.
  TITLE     Association of ISVsa3 with Multidrug Resistance in Salmonella
            enterica Isolates from Cattle (Bos taurus)
  JOURNAL   Microorganisms 11 (3), 631 (2023)
   PUBMED   36985205
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6988)
  AUTHORS   Moxley,R.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2023) School of Biological Sciences, University of
            Nebraska-Lincoln, 403 Manter Hall, Lincoln, NE 68588-0118, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 04-OCT-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 539X
            Sequencing Technology :: illumina MiSeq + Nanopore Gridion
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/25/2023 10:17:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,726
            CDSs (total)                      :: 4,608
            Genes (coding)                    :: 4,467
            CDSs (with protein)               :: 4,467
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 64 of 141
            Pseudo Genes (incomplete)         :: 82 of 141
            Pseudo Genes (internal stop)      :: 28 of 141
            Pseudo Genes (multiple problems)  :: 30 of 141
            Pseudo Genes (short protein)      :: 1 of 141
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117308.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Salmonella enterica subsp. enterica serovar
                     Derby"
                     /mol_type="genomic DNA"
                     /strain="RM004"
                     /serovar="Derby"
                     /isolation_source="Manila ropes placed above water tanks
                     and feed bunks used as sampling devices"
                     /host="Bos taurus"
                     /sub_species="enterica"
                     /db_xref="taxon:28144"
                     /plasmid="pRM004_1"
                     /geo_loc_name="USA: midwestern"
                     /collection_date="2000-10-30"
                     /collected_by="University of Nebraska-Lincoln"
     gene            join(6768..6988,1..70)
                     /locus_tag="PQQ01_RS23510"
                     /old_locus_tag="PQQ01_23510"
     CDS             join(6768..6988,1..70)
                     /locus_tag="PQQ01_RS23510"
                     /old_locus_tag="PQQ01_23510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047032.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            complement(62..340)
                     /locus_tag="PQQ01_RS23515"
                     /old_locus_tag="PQQ01_23515"
     CDS             complement(62..340)
                     /locus_tag="PQQ01_RS23515"
                     /old_locus_tag="PQQ01_23515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            462..884
                     /locus_tag="PQQ01_RS23520"
                     /old_locus_tag="PQQ01_23520"
     CDS             462..884
                     /locus_tag="PQQ01_RS23520"
                     /old_locus_tag="PQQ01_23520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001255518.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(1368..1601)
                     /locus_tag="PQQ01_RS23525"
                     /old_locus_tag="PQQ01_23525"
     CDS             complement(1368..1601)
                     /locus_tag="PQQ01_RS23525"
                     /old_locus_tag="PQQ01_23525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137427.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1608..2168)
                     /locus_tag="PQQ01_RS23530"
                     /old_locus_tag="PQQ01_23530"
     CDS             complement(1608..2168)
                     /locus_tag="PQQ01_RS23530"
                     /old_locus_tag="PQQ01_23530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2200..2637)
                     /locus_tag="PQQ01_RS23535"
                     /old_locus_tag="PQQ01_23535"
                     /pseudo=""
     CDS             complement(<2200..2637)
                     /locus_tag="PQQ01_RS23535"
                     /old_locus_tag="PQQ01_23535"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2846..3169)
                     /locus_tag="PQQ01_RS23540"
                     /old_locus_tag="PQQ01_23540"
     CDS             complement(2846..3169)
                     /locus_tag="PQQ01_RS23540"
                     /old_locus_tag="PQQ01_23540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3237..3428
                     /locus_tag="PQQ01_RS23545"
                     /old_locus_tag="PQQ01_23545"
     CDS             3237..3428
                     /locus_tag="PQQ01_RS23545"
                     /old_locus_tag="PQQ01_23545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4434..4565)
                     /locus_tag="PQQ01_RS23550"
                     /old_locus_tag="PQQ01_23550"
     CDS             complement(4434..4565)
                     /locus_tag="PQQ01_RS23550"
                     /old_locus_tag="PQQ01_23550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            4595..4885
                     /gene="cki"
                     /locus_tag="PQQ01_RS23555"
                     /old_locus_tag="PQQ01_23555"
     CDS             4595..4885
                     /gene="cki"
                     /locus_tag="PQQ01_RS23555"
                     /old_locus_tag="PQQ01_23555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            complement(4882..6528)
                     /gene="cka"
                     /locus_tag="PQQ01_RS23560"
                     /old_locus_tag="PQQ01_23560"
     CDS             complement(4882..6528)
                     /gene="cka"
                     /locus_tag="PQQ01_RS23560"
                     /old_locus_tag="PQQ01_23560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_011264160.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     Typing          complement(3514..3601)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          3612..4168
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.633"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1262..2856)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.624"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3229..3431)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.706"
                     /coverage="100.0"
                     /evalue="3.86e-12"
ORIGIN
        1 cgcccgtaaa tatctgaacg gtaaaaaggt tatggaagaa accggggaaa atacgccccc
       61 atcagcgtaa ggatttcttt tgccgctcca gagactccag ttttttacgc aaatcctctc
      121 ttttttgggc atctctggtg ccagccagct ctgctctcaa ctcatcgatc tgaagttgta
      181 tcttcagtct gttactgaac attttctgtc tggcattaac atccgcaaca atgccgtttt
      241 ttgtcttctc ggccttttgt tgaaaaacat tgctgtccgc atgactggca accgaagcag
      301 aaagaacact aaaaagcagg actggcacac attttttcac gggattattc ctgactcatt
      361 gaccatcaaa tcacattggg agtaaaccga cgtatgataa gagatactct tcggagatat
      421 aactccctga gtatcaagat taaaaacgca aggagatgtt tatgagatct gccgctgcca
      481 ggctgcttct gatacctctg ataacagcaa caatagctct tacaggatgc acaccaaaga
      541 ccagcctgga acgacatacc cggcattatg tttatgcttc agatgatgga tttgatccta
      601 acttctacac ccagaaagca gacaccatac gtatgatgct cccgttcttt cagcagttcc
      661 gggatatggg gatgaaagac aaagcagccg gagtatcagc agaaacggca cagcaacgtg
      721 taaaagaatt ccactcagaa aaattttttc actcactccg gagcacaaca gcctttgctg
      781 gcagaaaata cacaaacagc gatatgcctt cgccgaaaaa aatgaaacta atggcagaca
      841 ccatttctgc ggtttatctc gatggatacg agggcagaca gtaagggatt taccataatc
      901 ccttaattgt acgcaccgct gaaatgcgtt cagcgcgatc acggctgctg acaggtaaaa
      961 atggcaacaa accacccgaa aagctgccgc gatcgcacct gataaatttt aaccgtatgc
     1021 atagctattc agccatgtga ataacgctag ttttgcctgc gtaaacctca tgacactgtt
     1081 ttttttccat cttttcagtt gatgacatac gcagacatcg cgggatgagg ctgaggaatg
     1141 agcgcgatct ggcaaagagg caaaacacag caacaaaaac gacacgccag aatcgcgccc
     1201 ggatgcgttt ttaacgcgtt ccggtaccat ctggcaacct cccggaacaa ctcaccgtca
     1261 catccctatt gacgggccac gccatacccg tgcttcccgt tcctgctctt catgccagga
     1321 ccgcgcacgc tcccgttcca ggcgtgcctg cctttcctgt tcatccctta tctgctgttc
     1381 gtgataaata accgactcaa gtggtccacc tgcccggcta atctctgcac ctgctgactc
     1441 aagtcgtcgc actgttccct cagttgcccg ttctcctgtc gtgtcagctc gaacatatgc
     1501 tgcaaatccg tgaaggcgct ctcccagtct ttcagccgct gcgtatagtc ctgctgcaat
     1561 tgctctaagg cgttcagtaa gtgcatttcc agctctgtca tactcactta ctccctgacc
     1621 agtcttactg cgttcttctt ctccaccgtc cagttgtttt cccccttcac cccggacggc
     1681 aacaccagaa atttcccgtt cctgccctcg tgatacgtca caccccatgt tttttcccgg
     1741 agtttcgcca gcgtctcttc ctggtccctg atagccagga tgttcgccgc aatccggctt
     1801 tcctgccact gaatcagccc ccatgacgcc agaaaaatcc cgcccgtgac gcaaagcgcc
     1861 gtcagcgacg ggtacagtat ccgccctttg accagcttcc agagcagctc ttcctgccgc
     1921 cgggccagtt cgttctctgt ggcgctgaac tgcgcgttca cggcactgtt cagcgtctcc
     1981 agttgttctt tcaccgctgc tgtgtgtgcg ctgatagcgt ctctgatttt ctgcccgttt
     2041 aagttcagtt ccctgtctac agacgcttcg agcttcctga actcgctgtt cagcatgttc
     2101 tctgtagaga cggcacgctc tttcagtttc ttctcgaagt ctgtccccat ttgtaaaaga
     2161 ttgctcatac agcgcccctt tcagcctgag attacgccca ccctccgggt cggcgatact
     2221 gatactgctc ctggttgtcc tcacaacctc aaaacctgcc gctgtaagcg cctcagtgac
     2281 atcctgacgc gtttttagcg ctccggcatg gtaaagagcc tccagtcccc tcgtaatcgc
     2341 ttctgcggcc tcctgtttcg ctttcggcag attattcggg gtgacaagtg tccgcctgtt
     2401 ctccggtgcg ttcgggtcgt gcagcccgta atggtgattc accagtgtct gccaggcatt
     2461 gattcgcgga cggtctgctc ggtcgtaata gggctggagc cgttttccgc tcgccagctc
     2521 catattcggg atgacaaaat tcagctcaag acgccccttg tcctggtgct ccacccacag
     2581 gatgctgtac tgattttttt caagaccggg catcagtacc cgctcaaagc tctccatcac
     2641 cctttcacgc tctcccggtg gcagggtctg ctctgcaaaa gacagaaccc ccgaggtgta
     2701 ttttttcgca aacggcgtgg catcgatgag ttcccgcacc tcttcgggag caccccgcag
     2761 aactctcgcc ccttcccggt tacgctcccg gcccagcagg taatcaaccg gaccactgcc
     2821 accgcctttt cccctggcat gaaacttaac tatcatcccg ttctccctgt ttacggacct
     2881 catccctcag ctcactcagt tcacgtccga tggccatcag tgcagccacc acatgaaccc
     2941 ggtcatgccc cgaccactgt ccgctgttta tcttccgggc tatctgattc aggttattgc
     3001 cgaccgaagc gaactggcgc aacagcggcg gtgccagtgt cggaagacct gacgttttcg
     3061 atggcggtgc ccccaggcag accttacgca tccatgacgc aagttgtttt ccctcacaac
     3121 gtgccagcag ccgcgcatgt tcctcatccg tgacccgtat cgtgagcatc ctttcgcgtt
     3181 tcaccggtat cattaaaaac ctccgacaga ctccccacac atggagaaac agaactgtga
     3241 ctaaacagga aaaaaccgcc cttaacatgg cccgcttcat cagaagccag acgctgaccc
     3301 tgctggaaaa actgaatgaa ctggacgccg acgaccaggc tgacatctgc gaagcgcttc
     3361 acgaccacgc tgacgagctt taccgcagct gcctcgcacg cttcggggat gacggtgaaa
     3421 acctctgaca catgcagctc ccggagacgg tcacagctgg cctgtgagcg gatgccggga
     3481 gcagacaagc ccgtcagggc gcgtcagcgg gttttagcgg gtgtcggggc gcagccatga
     3541 cccagtcacg tagcgatagc ggagtgtata ctggcttaat catttggcat cagtgaggat
     3601 tgtatgaaaa gtgcaccatg ccgggtgtga aatgccgcac agatgcgtaa ggagaaaatg
     3661 ctcgtccagg cgcttttccg cttcctcgct cactgactcg ctccgctcgg tcgttcggct
     3721 gcggcgagcg gtatcagctc actcaaagac ggtaatgcgg ttatccacag aatcagggga
     3781 taacaccaaa agaaacatgt gagcaaaaaa caagaacccg gaaaaggcca cgcagctggc
     3841 gtttttccat aggctccgcc cccctgacga gcatcacaaa aaccgacgct caagtcagag
     3901 gtggcgaaac ccgacaggac ttaaagatac caggcgtttc cccctggtgg ctccctcgtg
     3961 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct ccctttggga
     4021 agcgtggcgc tttctcatag ctcacgctgt tggtatctca gttcggtgta ggtcgttcgc
     4081 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4141 aactatcgtc ttgagtccaa cccggtaaga cacgacttaa cgccactggc agcagccatt
     4201 ggtaactgga tagtggattt agatacgcag aactcttgaa gttgaagcct tatagcggct
     4261 acactggaag gacagcattt ggtatctgtg ctccactaaa gccagttacc cggttaagca
     4321 gtccccaact gacttaacct tcgactaaac cgcctcccca ggcggttttt tcgtttacag
     4381 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctactga
     4441 accgcgatcc ccgtcagttc agaagacgaa gatggtgcaa cggttcctcc ttgtacatca
     4501 cgaatatagt ttgcttgaca tcctgacaag aataatattg taattagaaa aattattatt
     4561 ttcatatttc tttccaacaa aagtaaaaat acttatgcat ttaaaatact acctacataa
     4621 tttacctgaa tcacttatac catggattct tattttaata tttaacgaca atgataacac
     4681 tcctttgtta tttatattta tatcatcaat acatgtattg ctatatccat actctaaatt
     4741 aaccatatct agatatatca aagaaaatac aaagttaaaa aaagaaccct ggtacttatg
     4801 caagttatct gcattgtttt atttattaat ggcaatccca gtaggattgc caagtttcat
     4861 atattacact ctaaagagaa attaaatccc taacaactca ttaagtttgc tcaactcatc
     4921 atcccctatg taagaagaaa caatacctgt gacaattgca atcccccaga aaccaagagg
     4981 cacaccaaca ataaaactaa acattaatgc cacaattttt gccacaccta catcgaccgc
     5041 acttttctcc agcgtgacaa acaaaggtcg ccagttacca gtttctattg catttttaaa
     5101 atcagaacca acatcatata gaaaagatac tcgactggtg attttaagcg actttgatat
     5161 cttcgttaaa ttcttagata actcatcata attaacagac tctaacgcat taaaaatagc
     5221 atcccgatca accttactga atttcttatc cagtacattc ttatactttt caaatgctgc
     5281 cagagcttca tcaacacctt gaattttctt tcctttagac ttttccgcta aatcctgagc
     5341 aatttttgcg tatttttctc catattgttc ggttatatat tgataaaatc caaccatagt
     5401 atcaacagca tcttttatct ggctcttttc aagagcatcc tgagcttctt ttaatttctg
     5461 ctctgcctct ttaacctcag cggccttacc atccctgaaa ctaacagcat tcttaagttc
     5521 attttcaact gcggagtact cagcctgctt agcttcaagc tgacgctgta gttttttctg
     5581 aacgtcacgc catcccggaa atccggtgac cttcacgtca acactcttct gagcgttttc
     5641 aagctctcct gcaactctgg aaacagtagc ttgcttactc tgtacatcac tttcagcctt
     5701 cgccagttca gctttcgcct cagctaaacg cttctctgct tccgccaccg cctgtttctc
     5761 ttcattaaga gtttcatgtt cagcgacctc tgcattttca cgagcctttt cttcagcttc
     5821 cttctgttta ttcacatcac caattttact ttgcaatgta tttttataag aattaagttt
     5881 gctaatatct gcatcaagct gatttgattt tttttgcaac tcatcaacat ccctctcaag
     5941 atcagtgata ccatgataag aatgatgctt gaatactttt ttcatctcct cgattttctt
     6001 ctgtttttca ctaatctgtg tagcaatttt attcttctgc ttttgttttt cattaattac
     6061 attacccacc accttcgaac tcttatccat atcactgacc tgagcattcg ttggtgcagt
     6121 attaactgaa gcagtgttat tgttgttatt tcctgagctt tctgcaaaaa gtgatggcat
     6181 atcactaatt aaagaattaa gaactctgga gacccctcca aatggattat caaccagagt
     6241 tgaattctct tctgtcataa caataccatt catcaccgga aggccatcat tattaatgac
     6301 aacatcaccc cacggagtca gatatgactc accggttttc atcacagttg atgtagaacc
     6361 agatgaattt gaatttccct gaccaccatt attaccatgc ccagagccac caccccagtg
     6421 aaccccacta ttactattat tatttccacc ttgctgattc agattcgccc ctgttccccc
     6481 catagactca ccagcagttg gtccatatcc acttagttct ttagccataa attcctcttt
     6541 gataattaaa acaataaatt aaaaacaata tactgtatat ataaccactg gttttatata
     6601 cagtaaaacc tactactcag cattgtccat gtcaagagca tggattttca tttttgcaat
     6661 aaggatcaca ctatggggag gcaggcattg agaacgtcga aacagaacac cggagcaaat
     6721 caggatgaga tataaaactg ttggatcatg aaaaaacgga gaacgatgtg agcaaatcac
     6781 cccgccataa actgaacaaa acagacaaac gacttctcga cacccttgtt gctgccggat
     6841 atgagcatga caaagcccgt gacctcatcc agaaacaggt ttacacgctg acactggctg
     6901 atcagcgtca tgtggtcagt gaaatcagta atggtgtgaa tcccacccag gcttactcgg
     6961 cggtatacca ggcaagacgc attcgcct
//
