LOCUS       NZ_CP117302             6988 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Salmonella enterica subsp. enterica serovar Derby strain RM006
            plasmid pRM006_1, complete sequence.
ACCESSION   NZ_CP117302
VERSION     NZ_CP117302.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32950811
            Assembly: GCF_028892955.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica subsp. enterica serovar Derby
  ORGANISM  Salmonella enterica subsp. enterica serovar Derby
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Lewis,G.L., Fenton,R.J., Moriyama,E.N., Loy,J.D. and Moxley,R.A.
  TITLE     Association of ISVsa3 with Multidrug Resistance in Salmonella
            enterica Isolates from Cattle (Bos taurus)
  JOURNAL   Microorganisms 11 (3), 631 (2023)
   PUBMED   36985205
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6988)
  AUTHORS   Moxley,R.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2023) School of Biological Sciences, University of
            Nebraska-Lincoln, 403 Manter Hall, Lincoln, NE 68588-0118, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 04-OCT-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 248X
            Sequencing Technology :: illumina MiSeq + Nanopore Gridion
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/25/2023 10:08:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,705
            CDSs (total)                      :: 4,587
            Genes (coding)                    :: 4,449
            CDSs (with protein)               :: 4,449
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 63 of 138
            Pseudo Genes (incomplete)         :: 78 of 138
            Pseudo Genes (internal stop)      :: 30 of 138
            Pseudo Genes (multiple problems)  :: 30 of 138
            Pseudo Genes (short protein)      :: 1 of 138
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117302.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Salmonella enterica subsp. enterica serovar
                     Derby"
                     /mol_type="genomic DNA"
                     /strain="RM006"
                     /serovar="Derby"
                     /isolation_source="Manila ropes placed above water tanks
                     and feed bunks used as sampling devices"
                     /host="Bos taurus"
                     /sub_species="enterica"
                     /db_xref="taxon:28144"
                     /plasmid="pRM006_1"
                     /geo_loc_name="USA: midwestern"
                     /collection_date="2000-10-30"
                     /collected_by="University of Nebraska-Lincoln"
     gene            complement(<66..305)
                     /locus_tag="PQP83_RS23440"
                     /old_locus_tag="PQP83_23440"
                     /pseudo=""
     CDS             complement(<66..305)
                     /locus_tag="PQP83_RS23440"
                     /old_locus_tag="PQP83_23440"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(514..837)
                     /locus_tag="PQP83_RS23445"
                     /old_locus_tag="PQP83_23445"
     CDS             complement(514..837)
                     /locus_tag="PQP83_RS23445"
                     /old_locus_tag="PQP83_23445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            905..1096
                     /locus_tag="PQP83_RS23450"
                     /old_locus_tag="PQP83_23450"
     CDS             905..1096
                     /locus_tag="PQP83_RS23450"
                     /old_locus_tag="PQP83_23450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2102..2233)
                     /locus_tag="PQP83_RS23455"
                     /old_locus_tag="PQP83_23455"
     CDS             complement(2102..2233)
                     /locus_tag="PQP83_RS23455"
                     /old_locus_tag="PQP83_23455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            2263..2553
                     /gene="cki"
                     /locus_tag="PQP83_RS23460"
                     /old_locus_tag="PQP83_23460"
     CDS             2263..2553
                     /gene="cki"
                     /locus_tag="PQP83_RS23460"
                     /old_locus_tag="PQP83_23460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            complement(2550..4196)
                     /gene="cka"
                     /locus_tag="PQP83_RS23465"
                     /old_locus_tag="PQP83_23465"
     CDS             complement(2550..4196)
                     /gene="cka"
                     /locus_tag="PQP83_RS23465"
                     /old_locus_tag="PQP83_23465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_011264160.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            4436..4726
                     /locus_tag="PQP83_RS23470"
                     /old_locus_tag="PQP83_23470"
     CDS             4436..4726
                     /locus_tag="PQP83_RS23470"
                     /old_locus_tag="PQP83_23470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047032.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            complement(4718..4996)
                     /locus_tag="PQP83_RS23475"
                     /old_locus_tag="PQP83_23475"
     CDS             complement(4718..4996)
                     /locus_tag="PQP83_RS23475"
                     /old_locus_tag="PQP83_23475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_274896577.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGARDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            5118..5540
                     /locus_tag="PQP83_RS23480"
                     /old_locus_tag="PQP83_23480"
     CDS             5118..5540
                     /locus_tag="PQP83_RS23480"
                     /old_locus_tag="PQP83_23480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001255518.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(6024..6257)
                     /locus_tag="PQP83_RS23485"
                     /old_locus_tag="PQP83_23485"
     CDS             complement(6024..6257)
                     /locus_tag="PQP83_RS23485"
                     /old_locus_tag="PQP83_23485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137427.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(6264..6824)
                     /locus_tag="PQP83_RS23490"
                     /old_locus_tag="PQP83_23490"
     CDS             complement(6264..6824)
                     /locus_tag="PQP83_RS23490"
                     /old_locus_tag="PQP83_23490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     Typing          complement(1182..1269)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          1280..1836
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.633"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4..524)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.425"
                     /coverage="99.0"
                     /evalue="6.22e-109"
     Typing          complement(897..1099)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.706"
                     /coverage="100.0"
                     /evalue="3.86e-12"
     Typing          complement(5918..6987)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.72"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gtaatcgctt ctgcggcctc ctgtttcgct ttcggcagat tattcggggt gacaagtgtc
       61 cgcctgttct ccggtgcgtt cgggtcgtgc agcccgtaat ggtgattcac cagtgtctgc
      121 caggcattga ttcgcggacg gtctgctcgg tcgtaatagg gctggagccg ttttccgctc
      181 gccagctcca tattcgggat gacaaaattc agctcaagac gccccttgtc ctggtgctcc
      241 acccacagga tgctgtactg atttttttca agaccgggca tcagtacccg ctcaaagctc
      301 tccatcaccc tttcacgctc tcccggtggc agggtctgct ctgcaaaaga cagaaccccc
      361 gaggtgtatt ttttcgcaaa cggcgtggca tcgatgagtt cccgcacctc ttcgggagca
      421 ccccgcagaa ctctcgcccc ttcccggtta cgctcccggc ccagcaggta atcaaccgga
      481 ccactgccac cgccttttcc cctggcatga aacttaacta tcatcccgtt ctccctgttt
      541 acggacctca tccctcagct cactcagttc acgtccgatg gccatcagtg cagccaccac
      601 atgaacccgg tcatgccccg accactgtcc gctgtttatc ttccgggcta tctgattcag
      661 gttattgccg accgaagcga actggcgcaa cagcggcggt gccagtgtcg gaagacctga
      721 cgttttcgat ggcggtgccc ccaggcagac cttacgcatc catgacgcaa gttgttttcc
      781 ctcacaacgt gccagcagcc gcgcatgttc ctcatccgtg acccgtatcg tgagcatcct
      841 ttcgcgtttc accggtatca ttaaaaacct ccgacagact ccccacacat ggagaaacag
      901 aactgtgact aaacaggaaa aaaccgccct taacatggcc cgcttcatca gaagccagac
      961 gctgaccctg ctggaaaaac tgaatgaact ggacgccgac gaccaggctg acatctgcga
     1021 agcgcttcac gaccacgctg acgagcttta ccgcagctgc ctcgcacgct tcggggatga
     1081 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagctggcc tgtgagcgga
     1141 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt tttagcgggt gtcggggcgc
     1201 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaatca tttggcatca
     1261 gtgaggattg tatgaaaagt gcaccatgcc gggtgtgaaa tgccgcacag atgcgtaagg
     1321 agaaaatgct cgtccaggcg cttttccgct tcctcgctca ctgactcgct ccgctcggtc
     1381 gttcggctgc ggcgagcggt atcagctcac tcaaagacgg taatgcggtt atccacagaa
     1441 tcaggggata acaccaaaag aaacatgtga gcaaaaaaca agaacccgga aaaggccacg
     1501 cagctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa ccgacgctca
     1561 agtcagaggt ggcgaaaccc gacaggactt aaagatacca ggcgtttccc cctggtggct
     1621 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1681 ctttgggaag cgtggcgctt tctcatagct cacgctgttg gtatctcagt tcggtgtagg
     1741 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1801 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttaacg ccactggcag
     1861 cagccattgg taactggata gtggatttag atacgcagaa ctcttgaagt tgaagcctta
     1921 tagcggctac actggaagga cagcatttgg tatctgtgct ccactaaagc cagttacccg
     1981 gttaagcagt ccccaactga cttaaccttc gactaaaccg cctccccagg cggttttttc
     2041 gtttacaggc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     2101 tctactgaac cgcgatcccc gtcagttcag aagacgaaga tggtgcaacg gttcctcctt
     2161 gtacatcacg aatatagttt gcttgacatc ctgacaagaa taatattgta attagaaaaa
     2221 ttattatttt catatttctt tccaacaaaa gtaaaaatac ttatgcattt aaaatactac
     2281 ctacataatt tacctgaatc acttatacca tggattctta ttttaatatt taacgacaat
     2341 gataacactc ctttgttatt tatatttata tcatcaatac atgtattgct atatccatac
     2401 tctaaattaa ccatatctag atatatcaaa gaaaatacaa agttaaaaaa agaaccctgg
     2461 tacttatgca agttatctgc attgttttat ttattaatgg caatcccagt aggattgcca
     2521 agtttcatat attacactct aaagagaaat taaatcccta acaactcatt aagtttgctc
     2581 aactcatcat cccctatgta agaagaaaca atacctgtga caattgcaat cccccagaaa
     2641 ccaagaggca caccaacaat aaaactaaac attaatgcca caatttttgc cacacctaca
     2701 tcgaccgcac ttttctccag cgtgacaaac aaaggtcgcc agttaccagt ttctattgca
     2761 tttttaaaat cagaaccaac atcatataga aaagatactc gactggtgat tttaagcgac
     2821 tttgatatct tcgttaaatt cttagataac tcatcataat taacagactc taacgcatta
     2881 aaaatagcat cccgatcaac cttactgaat ttcttatcca gtacattctt atacttttca
     2941 aatgctgcca gagcttcatc aacaccttga attttctttc ctttagactt ttccgctaaa
     3001 tcctgagcaa tttttgcgta tttttctcca tattgttcgg ttatatattg ataaaatcca
     3061 accatagtat caacagcatc ttttatctgg ctcttttcaa gagcatcctg agcttctttt
     3121 aatttctgct ctgcctcttt aacctcagcg gccttaccat ccctgaaact aacagcattc
     3181 ttaagttcat tttcaactgc ggagtactca gcctgcttag cttcaagctg acgctgtagt
     3241 tttttctgaa cgtcacgcca tcccggaaat ccggtgacct tcacgtcaac actcttctga
     3301 gcgttttcaa gctctcctgc aactctggaa acagtagctt gcttactctg tacatcactt
     3361 tcagccttcg ccagttcagc tttcgcctca gctaaacgct tctctgcttc cgccaccgcc
     3421 tgtttctctt cattaagagt ttcatgttca gcgacctctg cattttcacg agccttttct
     3481 tcagcttcct tctgtttatt cacatcacca attttacttt gcaatgtatt tttataagaa
     3541 ttaagtttgc taatatctgc atcaagctga tttgattttt tttgcaactc atcaacatcc
     3601 ctctcaagat cagtgatacc atgataagaa tgatgcttga atactttttt catctcctcg
     3661 attttcttct gtttttcact aatctgtgta gcaattttat tcttctgctt ttgtttttca
     3721 ttaattacat tacccaccac cttcgaactc ttatccatat cactgacctg agcattcgtt
     3781 ggtgcagtat taactgaagc agtgttattg ttgttatttc ctgagctttc tgcaaaaagt
     3841 gatggcatat cactaattaa agaattaaga actctggaga cccctccaaa tggattatca
     3901 accagagttg aattctcttc tgtcataaca ataccattca tcaccggaag gccatcatta
     3961 ttaatgacaa catcacccca cggagtcaga tatgactcac cggttttcat cacagttgat
     4021 gtagaaccag atgaatttga atttccctga ccaccattat taccatgccc agagccacca
     4081 ccccagtgaa ccccactatt actattatta tttccacctt gctgattcag attcgcccct
     4141 gttcccccca tagactcacc agcagttggt ccatatccac ttagttcttt agccataaat
     4201 tcctctttga taattaaaac aataaattaa aaacaatata ctgtatatat aaccactggt
     4261 tttatataca gtaaaaccta ctactcagca ttgtccatgt caagagcatg gattttcatt
     4321 tttgcaataa ggatcacact atggggaggc aggcattgag aacgtcgaaa cagaacaccg
     4381 gagcaaatca ggatgagata taaaactgtt ggatcatgaa aaaacggaga acgatgtgag
     4441 caaatcaccc cgccataaac tgaacaaaac agacaaacga cttctcgaca cccttgttgc
     4501 tgccggatat gagcatgaca aagcccgtga cctcatccag aaacaggttt acacgctgac
     4561 actggctgat cagcgtcatg tggtcagtga aatcagtaat ggtgtgaatc ccacccaggc
     4621 ttactcggcg gtataccagg caagacgcat tcgcctcgcc cgtaaatatc tgaacggtaa
     4681 aaaggttatg gaagaaaccg gggaaaatac gcccccatca gcgtaaggat ttcttttgcc
     4741 gctccagaga ctccagtttt ttacgcaaat cctctctttt ttgggcatct ctggcgccag
     4801 ccagctctgc tctcaactca tcgatctgaa gttgtatctt cagtctgtta ctgaacattt
     4861 tctgtctggc attaacatcc gcaacaatgc cgttttttgt cttctcggcc ttttgttgaa
     4921 aaacattgct gtccgcatga ctggcaaccg aagcagaaag aacactaaaa agcaggactg
     4981 gcacacattt tttcacggga ttattcctga ctcattgacc atcaaatcac attgggagta
     5041 aaccgacgta tgataagaga tactcttcgg agatataact ccctgagtat caagattaaa
     5101 aacgcaagga gatgtttatg agatctgccg ctgccaggct gcttctgata cctctgataa
     5161 cagcaacaat agctcttaca ggatgcacac caaagaccag cctggaacga catacccggc
     5221 attatgttta tgcttcagat gatggatttg atcctaactt ctacacccag aaagcagaca
     5281 ccatacgtat gatgctcccg ttctttcagc agttccggga tatggggatg aaagacaaag
     5341 cagccggagt atcagcagaa acggcacagc aacgtgtaaa agaattccac tcagaaaaat
     5401 tttttcactc actccggagc acaacagcct ttgctggcag aaaatacaca aacagcgata
     5461 tgccttcgcc gaaaaaaatg aaactaatgg cagacaccat ttctgcggtt tatctcgatg
     5521 gatacgaggg cagacagtaa gggatttacc ataatccctt aattgtacgc accgctgaaa
     5581 tgcgttcagc gcgatcacgg ctgctgacag gtaaaaatgg caacaaacca cccgaaaagc
     5641 tgccgcgatc gcacctgata aattttaacc gtatgcatag ctattcagcc atgtgaataa
     5701 cgctagtttt gcctgcgtaa acctcatgac actgtttttt ttccatcttt tcagttgatg
     5761 acatacgcag acatcgcggg atgaggctga ggaatgagcg cgatctggca aagaggcaaa
     5821 acacagcaac aaaaacgaca cgccagaatc gcgcccggat gcgtttttaa cgcgttccgg
     5881 taccatctgg caacctcccg gaacaactca ccgtcacatc cctattgacg ggccacgcca
     5941 tacccgtgct tcccgttcct gctcttcatg ccaggaccgc gcacgctccc gttccaggcg
     6001 tgcctgcctt tcctgttcat cccttatctg ctgttcgtga taaataaccg actcaagtgg
     6061 tccacctgcc cggctaatct ctgcacctgc tgactcaagt cgtcgcactg ttccctcagt
     6121 tgcccgttct cctgtcgtgt cagctcgaac atatgctgca aatccgtgaa ggcgctctcc
     6181 cagtctttca gccgctgcgt atagtcctgc tgcaattgct ctaaggcgtt cagtaagtgc
     6241 atttccagct ctgtcatact cacttactcc ctgaccagtc ttactgcgtt cttcttctcc
     6301 accgtccagt tgttttcccc cttcaccccg gacggcaaca ccagaaattt cccgttcctg
     6361 ccctcgtgat acgtcacacc ccatgttttt tcccggagtt tcgccagcgt ctcttcctgg
     6421 tccctgatag ccaggatgtt cgccgcaatc cggctttcct gccactgaat cagcccccat
     6481 gacgccagaa aaatcccgcc cgtgacgcaa agcgccgtca gcgacgggta cagtatccgc
     6541 cctttgacca gcttccagag cagctcttcc tgccgccggg ccagttcgtt ctctgtggcg
     6601 ctgaactgcg cgttcacggc actgttcagc gtctccagtt gttctttcac cgctgctgtg
     6661 tgtgcgctga tagcgtctct gattttctgc ccgtttaagt tcagttccct gtctacagac
     6721 gcttcgagct tcctgaactc gctgttcagc atgttctctg tagagacggc acgctctttc
     6781 agtttcttct cgaagtctgt ccccatttgt aaaagattgc tcatacagcg cccctttcag
     6841 cctgagatta cgcccaccct ccgggtcggc gatactgata ctgctcctgg ttgtcctcac
     6901 aacctcaaaa cctgccgctg taagcgcctc agtgacatcc tgacgcgttt ttagcgctcc
     6961 ggcatggtaa agagcctcca gtcccctc
//
