LOCUS       NZ_CP117236            24185 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Escherichia coli strain ATCC 25922 plasmid pATCC25922_3, complete
            sequence.
ACCESSION   NZ_CP117236
VERSION     NZ_CP117236.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32977649
            Assembly: GCF_028596225.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24185)
  AUTHORS   Johnson,J.D., Blankenship,H. and Soehnlen,M.
  TITLE     Long read genome assemblers struggle with small plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 24185)
  AUTHORS   Johnson,J.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2023) Bureau of Laboratories, Michigan Department
            of Health and Human Services, 3350 N Martin Luther King Blvd.,
            Lansing, MI 48906, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2022
            Assembly Method                   :: Trycycler v. v0.5.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 140x
            Sequencing Technology             :: Oxford Nanopore, Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028596225.1-RS_2024_03_23
            Annotation Date                   :: 03/23/2024 03:31:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,067
            CDSs (total)                      :: 4,951
            Genes (coding)                    :: 4,722
            CDSs (with protein)               :: 4,722
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 229
            CDSs (without protein)            :: 229
            Pseudo Genes (ambiguous residues) :: 0 of 229
            Pseudo Genes (frameshifted)       :: 99 of 229
            Pseudo Genes (incomplete)         :: 139 of 229
            Pseudo Genes (internal stop)      :: 51 of 229
            Pseudo Genes (multiple problems)  :: 52 of 229
            Pseudo Genes (short protein)      :: 4 of 229
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117236.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..24185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ATCC 25922"
                     /db_xref="taxon:562"
                     /plasmid="pATCC25922_3"
                     /geo_loc_name="USA"
                     /collection_date="2022"
     gene            1..843
                     /locus_tag="PQQ28_RS25125"
                     /old_locus_tag="PQQ28_25130"
     CDS             1..843
                     /locus_tag="PQQ28_RS25125"
                     /old_locus_tag="PQQ28_25130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435061.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_000435063.1"
                     /translation="MEQIKLHKQGGAAVIIIPASMIENTGWEDGTLLDVLEINEALHIR
                     RKTGSGALATKSCPSLFSSTMRHVNYDKIIRYNSGLNLLVNELSFSARRVFFICLYYYF
                     ENKLESDKSLLIRADKYAEFVGVDRSVAYKQMKDAADFFSRNKKLITHCDYISNEGLLR
                     VMFSSKTINYITAIDARKYQVTELPLKSALSLQGRYSWPLYQLIKFSHLKSFTIGVDEL
                     KKEIGYIQNQKFSFFKRDGINIAIDEITERTDVKNIKCVNAERQGRRVSKVRFEIEMR"
                     /name="replication initiation protein"
     gene            847..1290
                     /locus_tag="PQQ28_RS25130"
                     /old_locus_tag="PQQ28_25135"
     CDS             847..1290
                     /locus_tag="PQQ28_RS25130"
                     /old_locus_tag="PQQ28_25135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001074379.1"
                     /translation="MAKIYQFPQGDERGKFREEIARERKKRFAVKTGSTFVKWLGWTWF
                     YLRLLVASVLHFISVFVLAILGAFKWAVFWLGGILCVITWYHLDHQLWTPQNLTIPVIT
                     ATWIFGLLAEPLMELLNKTMPWHKLLVPEPAGESTENSSETSQ"
                     /name="hypothetical protein"
     gene            1361..1786
                     /gene="ddp1"
                     /locus_tag="PQQ28_RS25135"
                     /old_locus_tag="PQQ28_25140"
     CDS             1361..1786
                     /gene="ddp1"
                     /locus_tag="PQQ28_RS25135"
                     /old_locus_tag="PQQ28_25140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 3"
                     /protein_id="WP_000202958.1"
                     /translation="MTSLTPFRNVCPVTYSRFLSENFMKYQVREFITDKYAKAVNILKD
                     NLKENYHVFYGVRLSEILFPASEYGTDAFFKEFELINSVILPLVIFDLTQRKPMMIISF
                     DKIPDASLLEGTNIVVLECTTLADLLTNDNIGFLYKS"
                     /name="ddp1"
     gene            complement(1783..2760)
                     /locus_tag="PQQ28_RS25140"
                     /old_locus_tag="PQQ28_25145"
     CDS             complement(1783..2760)
                     /locus_tag="PQQ28_RS25140"
                     /old_locus_tag="PQQ28_25145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6685 family protein"
                     /protein_id="WP_001269914.1"
                     /translation="MRYLTELICQLYRLTDHHKLTAATFKNLADIKLVEPTADADTILQ
                     LENIFSEYSLRYIDRDLAEILSNIITTDKNHSLDFDFNKIQSLTASKSFGCGWNKVING
                     SWFKNLYSWGEGMYPAKNLKAENISDWEDNIWHIEHEGFNPRDPINVKYYSWLDRYVAL
                     NSGGSHHAAMVVYQSVRDKLEYKREAVIEQLSINSNTVEILGQNYYSFIFQIKHPSNKT
                     ENYTSEYEFTDALKDFGQNRHTTLKPVNYVSNIKLAFIPKDALKTNSETFRNWFYSAIS
                     YGKIISLPDYLKNPAHYHTYHYSHELDSITLGNPSRKYKLRGDS"
                     /name="DUF6685 family protein"
     gene            complement(2785..3066)
                     /locus_tag="PQQ28_RS25145"
                     /old_locus_tag="PQQ28_25150"
     CDS             complement(2785..3066)
                     /locus_tag="PQQ28_RS25145"
                     /old_locus_tag="PQQ28_25150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001185482.1"
                     /translation="MALKLNRPNIDESQQPATNAETARFISGATRAPVVGKPKLMNFRL
                     SAHFEDILEAEAIRTGQSKTTVLKAALAMYNSQDENIKNHWLLESAKF"
                     /name="hypothetical protein"
     gene            complement(3164..3826)
                     /locus_tag="PQQ28_RS25150"
                     /old_locus_tag="PQQ28_25155"
     CDS             complement(3164..3826)
                     /locus_tag="PQQ28_RS25150"
                     /old_locus_tag="PQQ28_25155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000516402.1"
                     /translation="MGKILLVVSDKGGVGKSTYVANTGSMLVNKGKSVIILKTDKNHDL
                     LSWNEKRTDNGLLTIPVHAAYGNVSNEIKRLSKLCEVLIVDCPGHDSQEFRSALTVSDI
                     VVTLVKPSSDFESETLTSVTEKIRTAQKANAALEPWVLFTRINSAKPRHRKAAIDLDKL
                     LRSDNIWIQPLKTRISELDVYESACNEGAGVHDVSRASSLSTAKAQIELVAQEIGIL"
                     /name="division plane positioning ATPase MipZ"
     gene            4207..4851
                     /locus_tag="PQQ28_RS25155"
                     /old_locus_tag="PQQ28_25160"
     CDS             4207..4851
                     /locus_tag="PQQ28_RS25155"
                     /old_locus_tag="PQQ28_25160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001503708.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000203201.1"
                     /translation="MTSNAPTNFVRAYLRASTAEQDASRALETIEAFARERGLIICSYY
                     IENESGSRLDRPELFRLLRDCQQNDILLIEDVDRLSRLSGEDWNTLKKMIRQKDIRVMA
                     VNVPTTWINSGMSEFDSRLFAAINDMLLDMLAAVARRDYEQRRERQKQGIEKARKDGKY
                     KGRKPNQARHDAIIRLIESGSSWTQVQKVLGCSRGTISSALKRKTGQKSSK"
                     /name="recombinase family protein"
     gene            5673..6155
                     /locus_tag="PQQ28_RS25160"
                     /old_locus_tag="PQQ28_25165"
     CDS             5673..6155
                     /locus_tag="PQQ28_RS25160"
                     /old_locus_tag="PQQ28_25165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001546353.1"
                     /translation="MRDKTREAMRMFLGGRCYTAENLERDFLSELTRYSDERWEAPQRG
                     ARLAAAVKRYKTSEMLRFIFATIAYDPDPDLTPLAVKRLCNALFGRTGSQWLIVELFGD
                     KERQHRSNDSTLEAVEKMAARYRHAARQHWAATLDEIERVKRLYQEGIKASRIKES"
                     /name="hypothetical protein"
     gene            6493..7104
                     /locus_tag="PQQ28_RS25165"
                     /old_locus_tag="PQQ28_25170"
     CDS             6493..7104
                     /locus_tag="PQQ28_RS25165"
                     /old_locus_tag="PQQ28_25170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000016733.1"
                     /translation="MSGRITTLCTAFGVVIAAVGLYLPYKNELNAALYQREFLTGKWST
                     DAEYIINSGDLGLDKPQSIMTIQLFVDKDGSIDGEFISEGLCDAMPLTWNITFNSDSPS
                     LINFIFARKFQIRQLVNGAMDKSPVVATLKLVDEDHKHNPIVFDVVNDSTGTLPKQITL
                     AKNLPKFEENYKYLQSYCANSTEKMYEKMMPEIRNLNKGL"
                     /name="hypothetical protein"
     gene            7196..7705
                     /locus_tag="PQQ28_RS25170"
                     /old_locus_tag="PQQ28_25175"
     CDS             7196..7705
                     /locus_tag="PQQ28_RS25170"
                     /old_locus_tag="PQQ28_25175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000190051.1"
                     /translation="MATEWVDVADNAVKIGLGALITIVGGWITLKLTHCHEIRKDAIAQ
                     RLRDIDKKTDRYIDFLSSSQTLMQKYLYESCNPGSEDYTNYMRLHNIVSLTSSPELRIY
                     AFDTQSKVSQFILLRKPNEPEVIDSCRNAARNAVSVLQGAVSAELLQDKAALQDKQHAW
                     WAFWRK"
                     /name="hypothetical protein"
     gene            7811..9712
                     /locus_tag="PQQ28_RS25175"
                     /old_locus_tag="PQQ28_25180"
     CDS             7811..9712
                     /locus_tag="PQQ28_RS25175"
                     /old_locus_tag="PQQ28_25180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000407093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000407093.1"
                     /translation="MEIVSFKVSQYRSISGEISSDNFNGLTIVGQNNSGKTNLLKAMRV
                     FFEGKFNPDLYDYEKDKPHGVKQGQTSFAVRFTFSESELDDVSEDLSQDDDDDDDDSII
                     ELGVLGGQEEPSNSISFDDGSSKQQESKPTQSRGRKLSLRDRYLQLFNMIDDSRLSSPQ
                     NEVTVHLTISNKNNYFYTVFKGYKRKPEVTSSMYTSVERRFIEKILSSFECVYIPSSKS
                     IDDIYHNLLVPYVRSEVASAMKTSVQVIHDKLLEVSEKITNEMILSGIDDCSFMIKAPP
                     SLDELFGRFEVSIKDTIENVISSKGMGLQCLSLFSSFKSISERKLTLGKKTIWMVEEPE
                     SFLHPSLGRSCNHIFKSLMDVAYVLITTHSLSFVSDEPSKIIELYKDDAGVTKDKKHEK
                     RFNAVASIRENLGVRFSDFFNISSSAVFVEGITDKQYIESILKLTSEHDEFKNRWPFLR
                     SAKVDEFGGVSQLGGFLKGCYEFISKDVPVISVFDGDEAGVKERTRLQSYFGKKQIRFE
                     SNKDYISVRSGFAIEGLFPDDFISDAMETHPSWFIGGKSVDADDVIEPFKVQDNKKTNL
                     LNFFLERCRVQPICGWISRWEKVFNVIDSALRDKSESITNKKRTEDTSGNTSDHQAA"
                     /name="AAA family ATPase"
     gene            complement(10117..10296)
                     /locus_tag="PQQ28_RS25180"
                     /old_locus_tag="PQQ28_25185"
     CDS             complement(10117..10296)
                     /locus_tag="PQQ28_RS25180"
                     /old_locus_tag="PQQ28_25185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000347020.1"
                     /translation="MDFMSILGRCLLLPFPVAMFVGCLFLGIDDRFTGMFMSFIVGCLS
                     WYITKPKPQKAEVA"
                     /name="hypothetical protein"
     gene            complement(10363..10458)
                     /gene="dqlB"
                     /locus_tag="PQQ28_RS25185"
                     /old_locus_tag="PQQ28_25190"
     CDS             complement(10363..10458)
                     /gene="dqlB"
                     /locus_tag="PQQ28_RS25185"
                     /old_locus_tag="PQQ28_25190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_000609147.1"
                     /translation="MINLLIIVLRAVVAVANALIAILELIRQFID"
                     /name="dqlB"
     gene            11223..12257
                     /locus_tag="PQQ28_RS25190"
                     /old_locus_tag="PQQ28_25195"
     CDS             11223..12257
                     /locus_tag="PQQ28_RS25190"
                     /old_locus_tag="PQQ28_25195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="WP_000973426.1"
                     /translation="MMIQSHPLLAAPLAVGDTIGFFSSSAPATVTAKNRFFRGVEFLQR
                     KGFKLVSGKLTGKTDFYRSGTIKERAQEFNELVYNPDITCIMSTIGGDNSNSLLPFLDY
                     DAIIANPKIIIGYSDTTALLAGIYAKTGLITFYGPALIPSFGEHPPLVDITYESFIKIL
                     TRKQSGIYTYTLPEKWSDESINWNENKILRPKKLYKNNCAFYGSGKVEGRVIGGNLNTL
                     TGIWGSEWMPEILNGDILFIEDSRKSIATIERLFSMLKLNRVFDKVSAIILGKHELFDC
                     AGSKRRPYEVLTEVLDGKQIPVLDGFDCSHTHPMLTLPLGVKLAIDFDNKNISITEQYL
                     STEK"
                     /name="S66 peptidase family protein"
     gene            complement(12314..14083)
                     /locus_tag="PQQ28_RS25195"
                     /old_locus_tag="PQQ28_25200"
     CDS             complement(12314..14083)
                     /locus_tag="PQQ28_RS25195"
                     /old_locus_tag="PQQ28_25200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231998.1"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]; GO:0008080 - N-acetyltransferase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_000057520.1"
                     /translation="MAQKITPSKIVKHARELIIKGIESGENSFVIFDVDGALERLDHYR
                     CQLKSFFPNSSIAYSYKSNNLAQWCQIISGKGLYAEVCSVDEMNLAKRDGFKRIVFDGP
                     LKKTSELLKAIEIGALIEVDNIDECKRLNELCKLHKLTCRIHLRLSHYYDDNLSRFGLS
                     ESEAINLLEMLISKSEYLILDGFHLHVGSNLPNAEKICKAIIQYHELILRYMPDDGTLN
                     LGSGIPADSFSASSDNPTPCPEVFFSSIYDTIKNCFGTVCDKWNYIFEPGRHLVEDFGY
                     FIGKVISTKNRYGVKVAQTNIGINWIPSIRNWDHSFTLFHNHNHISDDKSDEYIIAGFN
                     CFECDCLFPSVILPSNLSDYLFSVRGCGAYDMQTGNQWTRNLYAVYTITNDVVNISRIH
                     RRELDFRKYDVSLTPSGIKVNDEITLLYPALKYAEELYLLINQNKINFIKSMAWPAFVN
                     NISDSVSFIEQSMIDNQNEKALILFIKYKTKIAGVVSFNIIDHANKTAYIGYWLGANFQ
                     GKGIVTNAINKLIQEYGDSGVIKRFVIKCIVDNKKSNATALRCGFTLEGVLQKAEILNG
                     VSYDQNIYSKVIG"
                     /name="GNAT family N-acetyltransferase"
     gene            complement(14068..14871)
                     /locus_tag="PQQ28_RS25200"
                     /old_locus_tag="PQQ28_25205"
     CDS             complement(14068..14871)
                     /locus_tag="PQQ28_RS25200"
                     /old_locus_tag="PQQ28_25205"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152798.1"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]; GO:1904047 - S-adenosyl-L-methionine
                     binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_000152799.1"
                     /translation="MTIKHAEELTEEVILNMNYPDFVALMQQDNTPPGAEYTIDYWIKH
                     GCINKKSHLLDLACSTGFSSRECFKKEGASAEGIDISESAVMVANEKAKKLKANNLLKY
                     YVADACDLPFEDNSFTHVLGGCNFAFIQNRLIALNETHRCLNHMGSMCISNFYYRRKIS
                     DKLINDVYNAINFRPNPLWTLEYWHQFFSERFTLVSEENHEMESQSEDELKADIYDYIF
                     NRNEFTKGLNGSLQNVFFERFLKIRRPLNIQRDYQGVTLQIWRKK"
                     /name="class I SAM-dependent methyltransferase"
     gene            complement(14868..16082)
                     /locus_tag="PQQ28_RS25205"
                     /old_locus_tag="PQQ28_25210"
     CDS             complement(14868..16082)
                     /locus_tag="PQQ28_RS25205"
                     /old_locus_tag="PQQ28_25210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090465.1"
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="WP_001090465.1"
                     /translation="MNTANILSVNYGAYNAIRMLIGVYHAAFLISTGVTISQLAVLQIV
                     FSVTVLILDFPLSVIADRFYRKQVVVSGVFFTILFYPLCIQSPDMLALVFSEIFYAIGI
                     CCISGAIEGWVLSALDGRNEKFTLCAHLCERVSSFGSVITGAVGILVVYFYDSYKSGYI
                     VSFFLMFVVFIVFLCVKTNGSGGEKEARDSIIKQAKESIGLITGSYNGTYFLSITCMFT
                     IGIQVIYHFWQPIMLSGESIGSLSKRELITLLMCHIGAFSMQYIANYLMPKIDVRCKLY
                     ILVCTLCSFLSSIACILLFMLITDNKVVLGVAIFSIIHGMISTIPVGAQNIFISRLNGN
                     YKNIISGALGLVSLSCRVASISVLGVISVLPGNMPVTTYIFISAIACVLCGCLFLMWSF
                     KKEMV"
                     /name="MFS transporter"
     gene            complement(16079..17206)
                     /locus_tag="PQQ28_RS25210"
                     /old_locus_tag="PQQ28_25215"
     CDS             complement(16079..17206)
                     /locus_tag="PQQ28_RS25210"
                     /old_locus_tag="PQQ28_25215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="WP_000417394.1"
                     /translation="MEKYKCPLNATLYRVAYKSYLRGLFMDYILGRYVKIARYGSGGLV
                     GGGGKEQYVENLALWENIIKTAYCFITPSSYTAALETANIPEKDFSNCFRFLKENFFII
                     PGEYNNSTENNRYSRNFLHYQSYGANPVLVQDKLKNAKVVILGCGGIGNHVSVILATSG
                     IGEIILIDNDQIENTNLTRQVLFSEDDVGKNKTEVIKRELLKRNSEISVSEIALNINDY
                     TDLHKVPEADIWVVSADHPFNLINWVNKYCVRANQPYINAGYVNDIAVFGPLYVPGKTG
                     CYECQKVVADLYGSEKENIDHKIKLINSRFKPATFAPVNNVAAALCAADVIKFIGKYSE
                     PLSLNKRIGIWSDEIKIHSQNMGRSPVCSVCGNRM"
                     /name="ThiF family adenylyltransferase"
     gene            complement(17687..18151)
                     /locus_tag="PQQ28_RS25215"
                     /old_locus_tag="PQQ28_25220"
     CDS             complement(17687..18151)
                     /locus_tag="PQQ28_RS25215"
                     /old_locus_tag="PQQ28_25220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001443073.1"
                     /translation="MNMTARNAGLSFRSPDDDDSHRTRYSELQQAFDFYNQRLFDGELP
                     ECLITFQRVTQDLFQGKFALSWFDRFSVQVEQQKEMAAVIEQWRETIAQAHQNAEPGLI
                     LKRCCQCHRRTGAMITICLLRLLRTSQKGTKSNISVGVWCCCLGEVRTEY"
                     /name="hypothetical protein"
     gene            complement(18408..18815)
                     /locus_tag="PQQ28_RS25220"
                     /old_locus_tag="PQQ28_25225"
     CDS             complement(18408..18815)
                     /locus_tag="PQQ28_RS25220"
                     /old_locus_tag="PQQ28_25225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001546356.1"
                     /translation="MSLTLPLMYFQTGADLSKITHHEVHYQDGFVQQAWLQPEIQLFVV
                     CIARKKGSRREIKGGMAVLTSIPASNGAELAKALDNVMRQSDELRPLAGKDENVFIPAR
                     IGISGEEPPTEQELLSVFYQQCLKHGSWGSA"
                     /name="hypothetical protein"
     gene            complement(18870..>19223)
                     /locus_tag="PQQ28_RS25225"
                     /old_locus_tag="PQQ28_25230"
                     /pseudo=""
     CDS             complement(18870..>19223)
                     /locus_tag="PQQ28_RS25225"
                     /old_locus_tag="PQQ28_25230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059103.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0030527 - structural constituent of chromatin [Evidence
                     IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /name="H-NS family nucleoid-associated regulatory protein"
     gene            complement(19454..19726)
                     /locus_tag="PQQ28_RS25230"
                     /old_locus_tag="PQQ28_25235"
     CDS             complement(19454..19726)
                     /locus_tag="PQQ28_RS25230"
                     /old_locus_tag="PQQ28_25235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000160399.1"
                     /translation="MTKHDFVSFVSGELRQGAVRFSLAFNSKGEIVLHWTNKAGIRVWR
                     ILSGNRGKKPSKANLERMSNFRRWLFDARQGMEGYTQQPEQSNLS"
                     /name="hypothetical protein"
     gene            complement(19739..19888)
                     /locus_tag="PQQ28_RS25235"
                     /old_locus_tag="PQQ28_25240"
     CDS             complement(19739..19888)
                     /locus_tag="PQQ28_RS25235"
                     /old_locus_tag="PQQ28_25240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000003880.1"
                     /translation="MSERILSAINDVEKGGRPVFPLMPFHVFPEYMALLRKALEKKTQK
                     RTDK"
                     /name="hypothetical protein"
     gene            complement(19974..20153)
                     /locus_tag="PQQ28_RS25240"
                     /old_locus_tag="PQQ28_25245"
     CDS             complement(19974..20153)
                     /locus_tag="PQQ28_RS25240"
                     /old_locus_tag="PQQ28_25245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071526261.1"
                     /translation="MEREFSAKASLNRNIKFWFEQCGLSKERVIHCIDNWYDLAYPPSE
                     QEKVRTGESKKRSN"
                     /name="hypothetical protein"
     gene            complement(20196..20525)
                     /locus_tag="PQQ28_RS25245"
                     /old_locus_tag="PQQ28_25250"
     CDS             complement(20196..20525)
                     /locus_tag="PQQ28_RS25245"
                     /old_locus_tag="PQQ28_25250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000866648.1"
                     /translation="MKVLTFKNDTVSVGDIFVSSWGYEQTNVTFYQVLSVHGKKTVTVR
                     EIRANSEYTDSMVGFKTPVLNDFTGECFKRQIKDFGDELAIKIEDFETAYKTLPEEKHR
                     FSSYY"
                     /name="hypothetical protein"
     gene            complement(20618..20833)
                     /locus_tag="PQQ28_RS25250"
                     /old_locus_tag="PQQ28_25255"
     CDS             complement(20618..20833)
                     /locus_tag="PQQ28_RS25250"
                     /old_locus_tag="PQQ28_25255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001180116.1"
                     /translation="MQNKPTPEEVKNARVAAGLTLKEAADIFGYQLNSWQMKESAGKAS
                     RSLSIGEYQYLLLLANMHPSYRLVKK"
                     /name="hypothetical protein"
     gene            complement(20823..21068)
                     /locus_tag="PQQ28_RS25255"
                     /old_locus_tag="PQQ28_25260"
     CDS             complement(20823..21068)
                     /locus_tag="PQQ28_RS25255"
                     /old_locus_tag="PQQ28_25260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000356548.1"
                     /translation="MDKDKIIKKNRGNYSYVIRTMDEDGDTVFHVLKYVKTIDKTKSRK
                     TVRKLIMDEKLNLASLMLLDNWVLCDCLTKGDENAE"
                     /name="hypothetical protein"
     gene            complement(21113..21436)
                     /locus_tag="PQQ28_RS25260"
                     /old_locus_tag="PQQ28_25265"
     CDS             complement(21113..21436)
                     /locus_tag="PQQ28_RS25260"
                     /old_locus_tag="PQQ28_25265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001181903.1"
                     /translation="MQNYAKSVATEILRQLGGNRFIVMTGAKSFSYFDENGECGLTFRL
                     PSNFAMKGINLVKIKLDFTDTYQVKFSRVRGAEVKDISRFDNIYCDQLARLFTQETGLH
                     TVL"
                     /name="hypothetical protein"
     gene            complement(21582..21863)
                     /locus_tag="PQQ28_RS25265"
                     /old_locus_tag="PQQ28_25270"
     CDS             complement(21582..21863)
                     /locus_tag="PQQ28_RS25265"
                     /old_locus_tag="PQQ28_25270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694627.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000220560.1"
                     /translation="MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLH
                     GHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGRRESSEVYQIADLR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(21853..22104)
                     /locus_tag="PQQ28_RS25270"
                     /old_locus_tag="PQQ28_25275"
     CDS             complement(21853..22104)
                     /locus_tag="PQQ28_RS25270"
                     /old_locus_tag="PQQ28_25275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WP_000121743.1"
                     /translation="MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCV
                     PPKLYAYYRELAEDAELNAVADERMKNPEIVKVNLDDL"
                     /name="plasmid stabilization protein"
     gene            23326..24165
                     /locus_tag="PQQ28_RS25275"
                     /old_locus_tag="PQQ28_25280"
     CDS             23326..24165
                     /locus_tag="PQQ28_RS25275"
                     /old_locus_tag="PQQ28_25280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722379.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001546350.1"
                     /translation="MNLLSVTKKTKIRHRNEINSTFSSLPLSARRILFMAMAQIDSREE
                     LSEGATFRITAREYAFIADIDVTGAYRQLKEGAEELQASVIRIPRNQLLTAQGNQSSGH
                     TKKRGKLPSDAVRLMNLTAFCDYVESEGYIDIAFSHVIEPYITMLKDSYTTQVLISSVR
                     LADQNSNMLYQFIRKQYSSGKKTFFDIEVDVLKDELELFRIDNGEKVYLYQNFKDFNKV
                     FLQKNIEKINQYTEINHLEVKIIERVARRASKLRFSYKIDKESEGLDTRIPYGFRGA"
                     /name="replication initiation protein"
     BGC             6079..24185
                     /name="RRE-containing"
                     /gene="RRE-containing"
                     /tool="antismash"
     Typing          848..1293
                     /name="IncX1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.855"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggagcaaa taaaattaca taaacaaggt ggtgcggcag taattatcat tcctgccagt
       61 atgatagaaa ataccggatg ggaagatgga accttattgg atgtactaga aataaatgag
      121 gctcttcata taagaagaaa gacaggatca ggagctttag ctactaaatc ttgtccttct
      181 cttttttcat ctactatgag gcatgttaac tacgataaaa tcattagata taatagcgga
      241 ttaaatctgt tagttaatga actgtcattt tcggcaagaa gggtcttttt tatttgttta
      301 tattattact ttgaaaataa acttgaatcg gataagtcgc tattgatccg ggctgataag
      361 tatgcagaat ttgttggtgt tgatcgttct gtcgcatata agcaaatgaa ggatgcagca
      421 gatttctttt caagaaataa aaaattaatt acacattgtg actatatttc gaatgaggga
      481 ttgcttcgtg ttatgttttc gtctaagacg ataaattata ttacagcgat agacgcccgt
      541 aaatatcagg ttaccgagct tcctctaaaa tctgctttgt ctcttcaagg aagatactca
      601 tggcctttat atcaactgat aaaatttagc catcttaaat ctttcactat aggtgtagat
      661 gaacttaaga aagaaatagg ttatattcaa aatcaaaagt tttctttctt taaaagagat
      721 ggaatcaata tagctattga tgaaataaca gaaaggacag atgttaaaaa cattaaatgt
      781 gttaatgctg aaaggcaggg tagaagagtc agcaaagtcc gttttgagat cgaaatgagg
      841 taactcatgg ctaaaatcta tcaattcccc caaggggacg aacgcggtaa attcagggaa
      901 gaaatcgcca gagaacgtaa gaaacgtttt gcggtgaaaa cagggagcac ttttgttaag
      961 tggcttggat ggacctggtt ttatcttcgt cttctggtcg ccagtgtttt acattttatc
     1021 tctgtttttg tgttagccat tcttggcgcg tttaaatggg ctgtgttctg gctgggtggc
     1081 attctgtgtg tcataacctg gtatcatctc gatcaccagt tatggacgcc acaaaatctg
     1141 actataccgg tgattacggc aacctggatt tttggtctgt tggctgaacc gctgatggag
     1201 ctgctcaaca aaaccatgcc ctggcacaag ttactggttc ccgaacctgc gggcgaaagc
     1261 acagagaaca gcagcgagac gagccagtaa tgacgctact tagtcgttat gccagcccct
     1321 gccttttcac tgcgatgcag gatagggcaa acaccgcaaa atgacgtctc tgacgccatt
     1381 ccgcaacgtt tgcccggtga cctacagtcg gttcttgtcg gagaatttta tgaaatatca
     1441 ggtgagagaa tttattactg ataaatatgc gaaggctgtt aatattttaa aggataacct
     1501 taaagaaaac taccatgttt tttatggtgt gagattaagt gagattcttt ttccagccag
     1561 tgagtatggc actgatgctt tctttaaaga gtttgaatta attaacagtg taatacttcc
     1621 attagtaatc tttgatttaa cacaacgcaa gccaatgatg attatcagct tcgataagat
     1681 acctgatgcc agtttgttgg aaggaacaaa tattgtggtt cttgaatgta ctaccctcgc
     1741 cgatcttctg acaaatgata acattggttt tttatataaa agctaagaat ctcctctcag
     1801 tttatatttt ctgcttgggt tgccaagtgt tatcgagtct agttcatgtg agtaatgata
     1861 tgtatgataa tgtgctggat ttttcagata atcagggagt gatattattt tcccgtagct
     1921 aattgcagaa taaaaccagt tgcggaatgt ctcgctgttt gtttttaaag catctttggg
     1981 aataaatgct aattttatgt tagaaacgta attaacaggt ttaagtgtgg tatgcctatt
     2041 ttgtccaaag tcttttaaag catctgtaaa ttcatattct gatgtataat tttctgtctt
     2101 attactaggg tgttttatct ggaatatgaa tgaatagtag ttctggccaa gtatttcaac
     2161 ggtgtttgaa tttatgctca gttgttcaat aaccgcttct cttttatact ctaatttgtc
     2221 tctgacagat tgatatacaa ccattgctgc atgatgtgaa ccaccactgt ttaaagctac
     2281 atatcggtca agccaagagt aatatttaac attaatagga tcgcgagggt tgaaaccttc
     2341 atgctcaatg tgccatatat tatcctccca gtctgaaatg ttttcagctt tcaggttttt
     2401 ggcgggatac atcccttccc cccatgaata aaggtttttg aaccaactac catttattac
     2461 tttgttccat ccacaaccga aacttttcga ggcagtcaga gactgtatct tgttaaaatc
     2521 aaaatccaga gagtggtttt tatctgtagt tattatgtta ctcagaattt cagccagatc
     2581 cctgtcaata tatcttagtg agtattcgct aaatatattt tcaagctgca atatggtgtc
     2641 tgcatccgct gtaggttcaa ctaacttaat atcagcaaga tttttaaacg ttgcagcggt
     2701 cagtttatgg tgatctgtca acctgtagag ttgacagatt aattcggtta aatatctcat
     2761 agataatcat ccatacttct atggttagaa ttttgccgat tccagtaacc agtgattctt
     2821 tatgttttca tcctggctgt tgtacatcgc cagcgctgct ttgagaacgg ttgttttgct
     2881 ctgccctgtt ctgatagctt cagcttccag tatgtcctca aaatgggctg aaagtctgaa
     2941 gttcatcaac tttggtttac ccactacagg cgctctggta gcgccggaga taaaacgcgc
     3001 ggtttctgcg tttgttgctg gttgctgaga ttcatcgatg ttcggacggt ttagttttaa
     3061 agccatgaca atttcccctt gtacggtatg cattaatagt aaatatcata ctgtctttat
     3121 attgtcttta caatgtaaag atttatagat ggtattgata ttactataaa attccaattt
     3181 cctgcgctac tagttcaatc tgtgctttcg ctgttgaaag gcttgatgcc cggctaacgt
     3241 catgtacccc tgccccttca ttacaggcgc tttcatacac atccagctcg gatatccggg
     3301 ttttaagagg ttgaatccag atgttgtccg accgcagtaa tttatccaga tcaatggccg
     3361 cttttctgtg gcggggtttt gctgagttga ttctggtgaa cagcacccac ggttccaggg
     3421 ctgcgttagc tttctgagcg gtgcggattt tctctgtaac actggtcagt gtttcgcttt
     3481 cgaaatcaga ggacggctta accagtgtta ccacgatatc tgatactgtc agggcgctac
     3541 ggaactcctg gctgtcgtgg ccggggcaat cgacaatgag gacttcacac agcttactaa
     3601 gtcgcttaat ttcattgctg acgtttccgt aagctgcgtg aaccgggata gtgagtaagc
     3661 cgttatctgt ccgcttttcg ttccagctca acagatcgtg gtttttatct gtcttgagga
     3721 taattaccga cttaccttta ttgaccagca ttgagcctgt gttagccacg taggtacttt
     3781 ttcctacacc acccttatct gatacgacaa gcaagatttt acccatttga gaattcctct
     3841 ctttacgatg taaagattat gtaaagatcg tcttgtcttt acattgtgtg ttttccgtaa
     3901 agatgatttt gtctttacag tgtctttata ttacatagga tttaccatgt aaagcacaaa
     3961 taaagacaat tatgtattta tgttgtcttt atattgtaaa gattagtgtg tctttacagt
     4021 gtctttatat gataaatcag cacttgtctt tacatcatct ttatgttgta aatttccatg
     4081 tgacattgtc actttatgac cagaggtctg ttttctggtt cttttagagt gtgcctttcc
     4141 tgtcgatcca ctttctggtt cattttgggt atagttgtgt gaccttatgc gaactggttc
     4201 tctaatatga cttcaaatgc accaacaaac tttgtccgcg cttatctgag agcttcaaca
     4261 gcagaacagg atgcttcgcg tgccctggaa actatcgagg cttttgccag ggaaagaggc
     4321 ctgatcattt gcagctatta tattgagaat gaatccggct cccgtcttga ccgcccggaa
     4381 ctcttccgtc ttctgagaga ttgccagcaa aatgacattt tgcttatcga ggatgtggac
     4441 agactttccc gactgtcagg ggaagactgg aatacgttga agaaaatgat ccggcagaaa
     4501 gacattcggg ttatggcggt gaatgtgccg acgacatgga ttaattctgg tatgagtgag
     4561 tttgacagcc ggttgtttgc agccattaac gatatgctgc tggatatgct ggccgcagtt
     4621 gccagaaggg actatgaaca acgtcgggaa cgccagaaac aagggataga gaaagcaagg
     4681 aaagacggta aatataaagg gcgtaaacct aatcaagcca gacatgatgc aattatcagg
     4741 ttgatagaaa gcggtagctc ctggacacag gtacagaaag tgctagggtg cagccgtggc
     4801 acaataagca gtgcgctaaa acgtaaaacc gggcaaaaat ccagtaagta gagggggtgg
     4861 ggggctaata tatctgaaag gcaggatatt aactcaggag gttgggtgtg tggtgtttag
     4921 ttaatgcatt tgaatgttgt aactaaaata tgattttatt aaaggccggt ggtggttagc
     4981 ccccggcctt agttacgtct ccgccatcag cccatcaact ggagaaggta gcgaatcagt
     5041 tcaagaagcg agattagcgc ccccagaacg atgccgttac cgacattatc atgaatcccg
     5101 ttttttcagt aagttgcagc atacaaagta attaaaaaaa cgtcattttt tgttgatgga
     5161 tgcagtagcg gtggtgcgct acgtgaaatc tggaaaatgt tggcggggta agtgactcaa
     5221 tttttaaatc tccgctcctg ttttttgggg atgtgtcagc aattatgact tggagcaaag
     5281 gtgtgtccga caacaaaaat tctgatcaca aaaaaatttt ggacattttt tccgataatg
     5341 catttttgtc catgatgaat gaacgacagg gcagggaaag tcgcgcaaga ggcagcggac
     5401 tgaatcagcc cttcacacac aatggcacac ggaaagacac gtttgtgtct gtcccgaaga
     5461 gtgtttctga aatacagggc acgacagcga tgtatgcacg taataaattc cgtgtataaa
     5521 cgacgcgctc agggggggga atccctgata cccccgctca gtttctgcaa aagttcgctg
     5581 tatggaggtt ctacgaacca tgatatttgt ttctattgat acggcttaaa gctaatgtac
     5641 gcatacatta gcattaatcc agagaataat acatgagaga caaaaccaga gaagctatgc
     5701 gtatgtttct cggtggcaga tgttacaccg ctgaaaatct tgaaagggat ttcctttcag
     5761 aactgaccag atacagcgat gaacgctggg aagccccgca acgtggcgca cgtctggcgg
     5821 cagcggtgaa gcgttacaaa acctcggaaa tgctgcggtt tatttttgcc accatagcat
     5881 atgaccctga cccggattta acaccgctgg ccgttaaacg gttgtgcaat gcgttgtttg
     5941 gcagaacggg gagccagtgg ctgattgtgg aattattcgg ggataaggag cggcagcacc
     6001 gcagcaacga cagcaccctg gaggccgtgg aaaaaatggc tgccagatat cgtcatgcgg
     6061 caagacagca ctgggcggca acgctggacg aaattgaacg ggtaaagcgt ctgtatcagg
     6121 agggaatcaa ggcatccaga ataaaagaga gttaactccc tccccattaa tgctagtgta
     6181 cgcgtacatt agcattaaac ctccagccac acacaaatcc acatgtttcc acccgtccgg
     6241 tggttaagtt ccctgccgta aacaccagtg aacgccgggc aggacgaaaa ggaagcgatt
     6301 atcctgaata acctaggata tgggcacata tgccggaaca gggcacagga taaaggaact
     6361 aaacatatga atgttgtaac taattgagtc taaacaaaaa aacaaaatgt ccaatatggg
     6421 cgggggatta ctaaaggagg ctattaatgt acaattggtt ataaatttta catatgaaga
     6481 taagggaaaa aaatgagtgg acgaatcaca accttatgca ctgcatttgg ggtggttatc
     6541 gcagcagtgg gactttacct accatataaa aatgaattga atgctgctct ttatcaaaga
     6601 gaattcctta ccggaaaatg gtcaacagat gctgaatata ttattaatag tggggatctt
     6661 ggactagata aaccgcaatc aattatgact atacaattat ttgttgataa agatggttct
     6721 attgatggtg aatttatctc agaaggatta tgtgatgcaa tgcctttgac gtggaatata
     6781 acttttaata gtgacagtcc atctctaatt aattttattt ttgcgagaaa attccaaatt
     6841 agacaactag ttaacggggc aatggataaa agccctgttg tagctacatt aaaattagtt
     6901 gatgaagatc ataaacataa tccgatagtg tttgatgtgg tcaacgattc taccggaacc
     6961 ttacctaaac agatcacttt agctaaaaac cttccgaaat tcgaagaaaa ctataaatac
     7021 ctacagagtt attgcgctaa ctcaactgaa aagatgtacg aaaagatgat gccagagatc
     7081 aggaatttga acaaaggttt ataacgtttc ggtgctagac agaaaatgct gtatgtccag
     7141 tcagccggag aattatacta gcgaccatga tcatcacagg aagaacgtgt aacagatggc
     7201 aactgagtgg gtagatgtgg ctgataatgc cgttaaaatt gggctgggtg cattaatcac
     7261 cattgttggt ggctggatta cactgaaatt aacacactgc cacgaaataa gaaaagatgc
     7321 aatagctcag cgactgcggg atatagataa aaaaactgac cgctatatag attttctttc
     7381 ttcatcgcaa acgctaatgc agaaatatct ttatgaatca tgtaatccag gtagcgagga
     7441 ctacaccaat tatatgcggt tacataatat tgtaagcctg acatcctccc ctgaattgcg
     7501 aatctatgct tttgatactc agtccaaagt atcgcaattt atcctgctca gaaagcccaa
     7561 cgaaccagag gttattgatt cctgtcgcaa tgccgcgaga aacgccgttt ctgtgcttca
     7621 gggggcagtg agtgctgaat tgcttcagga taaagcagca cttcaggaca aacaacatgc
     7681 atggtgggct ttctggcgaa aataaagcag aacagtggag gaagagccat ttctccaagt
     7741 agcgaaggac agttaaagcc ttgggaaaaa atgtcgataa tccgagcaac attctatcgt
     7801 aggaagaaaa atggaaatag tatcatttaa agtttctcag tataggtcta tttcagggga
     7861 gatatcctct gataacttta atggattaac cattgttggg caaaacaaca gtggtaaaac
     7921 aaacctatta aaagctatga gggttttttt tgaaggaaaa tttaatcctg acttatatga
     7981 ctatgaaaaa gataagccac atggtgttaa gcaaggacaa actagttttg ctgtaagatt
     8041 tactttttct gaaagtgagc tagatgatgt ctctgaggat ttgagccaag acgatgatga
     8101 tgacgatgat gactcgatta ttgaacttgg tgttttaggg gggcaagaag aacctagtaa
     8161 ttctatttca tttgatgatg gcagctctaa acaacaagaa tcaaaaccaa ctcaatcaag
     8221 aggcagaaag ctaagtttga gagatagata tttgcagcta tttaacatga ttgatgactc
     8281 caggttaagt tcacctcaaa acgaagtgac cgtacatctg acaatatcta ataaaaacaa
     8341 ttacttctac actgtcttta aaggttacaa gaggaaacct gaagtaactt cctccatgta
     8401 tacatctgtt gaaagaaggt tcatagaaaa aatactttct agttttgagt gtgtgtatat
     8461 accatcgtca aaaagtattg atgatatata tcataatcta ttagttccat atgttaggag
     8521 tgaagtagct tcagccatga aaacatcggt tcaagtgatt catgataaac tgttggaagt
     8581 gtctgaaaag ataacaaatg aaatgattct aagcggtata gatgactgta gctttatgat
     8641 taaagctccg ccatctcttg atgagttatt tggtcgcttt gaggtttcaa taaaagatac
     8701 tattgagaat gtgatatcga gtaaaggtat ggggctacaa tgcttatctt tattttcttc
     8761 atttaaatct atttcagaga gaaaacttac tctaggtaag aaaacgattt ggatggttga
     8821 ggagcctgag tctttcttgc atccctcatt aggccggtca tgtaatcaca ttttcaaatc
     8881 actgatggat gttgcttatg ttcttattac aactcattcc ttatcatttg tcagtgatga
     8941 gccaagtaag atcattgagt tatataaaga tgacgccggt gttacaaaag ataaaaagca
     9001 tgaaaaaaga tttaatgcag ttgcaagcat aagagaaaat cttggggtca gattttctga
     9061 tttctttaac atatcaagta gtgcagtttt cgttgaaggt atcaccgaca aacaatatat
     9121 agaatcaata ttgaaattga catctgaaca tgatgagttt aagaacagat ggccttttct
     9181 gaggagtgct aaagttgatg agtttggtgg agtcagtcaa cttggaggat tcttgaaagg
     9241 gtgctacgag tttatttcta aagatgtccc cgtaatatct gtttttgacg gtgatgaagc
     9301 tggggtcaaa gaaagaacca gattacaaag ctactttggt aaaaaacaaa ttagatttga
     9361 gtcaaataaa gactatattt ctgtaagaag tggttttgct attgaaggcc tcttccctga
     9421 cgatttcatt tctgacgcaa tggaaaccca tccgtcgtgg tttatcggag gaaaatctgt
     9481 tgatgccgat gatgtaattg aaccattcaa agtgcaagat aataaaaaaa ctaacttatt
     9541 aaatttcttc ctggaaagat gtcgtgttca acctatatgc ggttggattt ctcggtggga
     9601 aaaggtgttt aacgtcatag actcagcctt gcgagataaa agtgaatcca tcacaaacaa
     9661 aaaacgaacc gaagatactt ccggtaacac ttcagatcat caagctgcat aatcgattgt
     9721 gtacaccaag gttgtacata cacctgatta atcaagagtg agaccataac aagcctatat
     9781 cagataacag ccctcttcat aagagggctg tctttttcgc gggcatcgta cttgggtttc
     9841 acttggaaaa tggaaaacat cctacgctaa gagcgttttt taaacaaccg gcttgttggt
     9901 ttttacgaga tactgtgcag ttgaaggaac aagataaagg aattaaagac cctttagtac
     9961 catactttca tgaaattttc ccatattttc ttttagatcg tgcaacagcg aaaagacgta
    10021 ggaattggct tgattagccg caatactatt tctggacaga atgagtgtat atccaagtcg
    10081 ggcgaggaat taaacgaggg gcgtaaaaaa ggccgcttac gcgacctcag ccttttgcgg
    10141 ttttggcttg gtgatatacc acgatagaca accgacaata aaactcatga acattccagt
    10201 aaaacgatca tcaatgccta gaaacaggca accgacaaac atcgcaacag ggaaaggaag
    10261 aagtaaacag cgccccaaaa tgctcataaa atccataaat cctcctttgt taaagggcag
    10321 gaagcggttc acttcctgcc cttagttacg tttccgctgt catcagtcga tgaactggcg
    10381 aatcagttcc agaattgcaa tcagcgcgtt tgcaacggcg accacagccc tgagaacgat
    10441 gattaacaaa ttaatcatcc gtttctccag taaaactgga ggaaatcacc gttgcttact
    10501 catacactgc ctgtcacaac ggctctctcg cattgttagg tgtagagtga cctcctcctg
    10561 ccagagcacc gaatacagca ccacctgttt atccagccag gactttaaaa tcgttcatac
    10621 ccgcagatac gggaagagca ggattgcaag tcaactacat gactaactga ctgattatac
    10681 gctacacaaa acacccgtta caccactaaa tgtggttcct taaccacatt tagttgcttt
    10741 acagcaccat agtgaataaa atgtaaacgt taggtgtaga gtaaccttct ccagtcagag
    10801 ccaccaactc agggctggaa aacacaaaaa agcccgatta ccagtcgggt ttttttgtgc
    10861 ctgtcgttca gctgtacttc cgttttttcc ccttcatttg agggattttt ctttcatttt
    10921 ttgttgcgac caatactgct gatcgcgttg gccaacagat caataccgtt atcggcgttt
    10981 tcatgaatcc atttttttca gtaagttgca gcacaacaga tgattaaaaa agctatcagt
    11041 ttttttgatg gatacagtag tggcggtgcg ctacgtgaaa tctgaggctt ttcggggggg
    11101 attggacgag aaaatttacg agagaacact gcgattttca aaaaaagccc ataaactgaa
    11161 agtgctactg acatatctca aaacgggttc attttgacac taaatggaaa ggaaaaatcc
    11221 tcatgatgat acaatctcat ccactactgg ccgctcccct ggcagtagga gatacaattg
    11281 gtttcttttc atcatctgct ccggcaacag ttactgcaaa aaatcgtttt tttcggggag
    11341 ttgagtttct tcagagaaag ggatttaagc tggtatcagg gaagcttacc ggtaaaacag
    11401 atttttatcg ttcaggtact attaaagaaa gagctcaaga atttaatgag ttagtctaca
    11461 atcctgatat tacctgtata atgtcaacga tcggtggaga taacagtaat tcactactac
    11521 cgtttctgga ctatgatgct atcattgcaa accccaaaat tatcataggt tactcagata
    11581 caactgcttt attagcagga atatatgcaa aaacagggtt aataacattc tatggaccag
    11641 ctcttattcc ttcgtttggt gaacatccac ctcttgtgga tataacatat gaatcattta
    11701 ttaaaatact aacaagaaaa caatcaggaa tatataccta cacattacct gaaaagtgga
    11761 gtgatgagag cataaactgg aatgaaaaca agatattaag gcctaagaag ctatataaaa
    11821 acaactgtgc cttttatggt tccggaaaag ttgaggggcg tgtaattgga ggaaatctaa
    11881 atactttgac aggtatatgg gggagtgaat ggatgcctga aattcttaat ggagatatat
    11941 tgtttattga ggacagtcgg aaaagcattg caacaattga acgattattc tctatgctaa
    12001 agcttaatcg cgtgtttgat aaagttagtg caataatact cgggaaacat gagctttttg
    12061 attgtgcagg aagtaaacgc agaccatatg aagtattaac agaggtatta gatgggaaac
    12121 agattcctgt actggatgga tttgattgtt cacatacaca tccaatgcta actcttccac
    12181 ttggtgtaaa attagctatt gactttgaca acaaaaatat atctataaca gaacaatatc
    12241 tatctaccga gaaataaata ttatcatctc acagacataa acaaaatggc gtagaatact
    12301 acgccataca gtattaacca attacttttg aataaatatt ttgatcgtat gatacaccgt
    12361 tgagtatttc tgctttttgc agaacacctt ctaaggtgaa gccacacctc aatgccgtgg
    12421 cattactttt tttattatca acaatacatt ttataacaaa tctttttata acgcccgaat
    12481 ccccatactc ctgtatcagt ttatttatag cattggttac aatccccttc ccctgaaagt
    12541 tagcacctaa ccaatagcca atatatgctg ttttattggc atggtcaata atattaaacg
    12601 atacaacgcc agctatctta gttttgtatt taataaagag gattaatgct ttttcattct
    12661 ggttatcaat cattgattgc tcaatgaaag aaacagaatc agaaatgtta ttaacaaacg
    12721 ctggccatgc catgctttta atgaaattta ttttgttttg attaatgagc aaatataact
    12781 cttcagcgta ttttaacgct ggatacaata atgtaatttc atcatttact tttattcctg
    12841 atggcgtaag agatacatca tacttccgga aatcaagttc tcttctgtga atccttgata
    12901 tattaacaac atcatttgtt atggtatata cagcataaag attacgcgtc cactggtttc
    12961 ctgtttgcat atcataagca ccacaccctc gaacagaaaa taaataatca gataagttac
    13021 tgggaagaat aactgaagga aacaagcaat cacattcaaa gcaattgaat ccagcaataa
    13081 tatactcatc agatttatca tctgaaatat ggttatgatt atgaaacaat gtaaatgaat
    13141 ggtcccaatt tctgatcgaa ggtatccagt ttataccaat gttagtttga gcaactttaa
    13201 caccatacct gtttttagta ctaataactt tccctataaa gtagccaaag tcttcgacca
    13261 gatgtctccc tggttcaaaa atatagttcc atttatcaca tacagtaccg aagcaatttt
    13321 ttattgtatc atatatcgat gagaaaaata cctccggaca tggagttggg ttatcactgg
    13381 atgctgagaa agaatctgca ggtatcccac taccaagatt aagagtgcca tcatctggca
    13441 tatacctaag aatcaactca tgatactgta taatagcttt acatattttt tccgcattgg
    13501 gtagattaga gccaacatgt aaatggaacc cgtctaaaat gagatactct gacttactga
    13561 tcaacatctc cagtaaatta atagcctcac tttcagataa gccaaatctt gataaattat
    13621 catcatagta atgcgataat ctcaaatgaa ttctacatgt cagcttatgc aacttacata
    13681 gttcattaag tcttttacat tcatcaatat tgtcaacttc aattaatgcc ccaatctcta
    13741 ttgcctttaa tagttcactc gtttttttta aagggccatc aaaaacaatc cgcttaaaac
    13801 cgtctctttt ggcgaggttc atttcatcaa cagagcacac ctctgcatac aaacctttac
    13861 ctgagataat ctggcaccat tgtgccagat tatttgactt atatgaatat gcgatactac
    13921 tgttggggaa aaaactctta agttgacacc tgtaatgatc cagacgctcc agagctccat
    13981 ctacatcaaa aatgacaaat gagttttcgc ccgattcaat ccccttaata attaactccc
    14041 gggcatgttt aacaatttta gatggtgtta ttttttgcgc catatttgca atgtaactcc
    14101 ctgataatct ctttggatat ttaatggtct gcgtattttt aaaaatcttt caaaaaatac
    14161 attctgaagt gaaccattca aaccttttgt aaactcattt ctattgaaaa tgtaatcata
    14221 aatatcggct tttaactcat cttcactttg agattccatc tcatggtttt cttctgaaac
    14281 aagagtaaat ctttcagaaa aaaactgatg ccagtattcc aaagtccaca aaggattggg
    14341 tctgaaattt attgcattat atacatcatt gataagttta tctgatattt tacgcctgta
    14401 ataaaaattt gaaatgcaca tgcttcccat gtggtttaaa catcgatgag tttcattcaa
    14461 agctattaac cgattttgta taaatgcaaa attacatcca ccgagaacat gtgtaaaaga
    14521 gttatcttca aatggtaaat cacaagcatc agctacataa tatttcaata aattattagc
    14581 cttaagcttt tttgcctttt catttgcgac cattacagct gattcagata tatcaatacc
    14641 ctctgcagat gcaccttctt ttttgaagca ttcacgtgaa ctaaaacccg tggaacatgc
    14701 taaatctaat aggtgagact ttttgttaat acatccatgc ttgatccagt agtctatggt
    14761 gtactctgct ccaggaggag tgttatcctg ttgcatcaga gcaacaaaat cagggtaatt
    14821 catatttaaa ataacttctt ccgtcaattc ttcagcatgt ttaatcgtca taccatctcc
    14881 tttttaaagg accacattaa gaacaaacaa ccgcacagta cacatgcaat tgcagagata
    14941 aatatataag tggtgacagg catattacct ggtaatacag atataactcc caacacagaa
    15001 atggaagcaa cacgacatga aagagaaaca agaccaagag ctcccgaaat aatattctta
    15061 taattgccat taagtctgct tatgaatata ttctgtgctc caacaggtat tgttgaaatc
    15121 atcccatgga ttatagagaa aatagctacg ccgagaacaa ccttattatc tgttattaac
    15181 ataaatagta aaatacaggc gattgaacta agaaatgaac ataatgtaca aacaagtata
    15241 taaagtttac accttacatc tatctttggc ataagatagt ttgctatata ttgcattgaa
    15301 aatgcaccaa tatgacacat cagtaaagtt ataagctctc tttttgataa tgagccaata
    15361 ctttccccag atagcataat tggctgccaa aaatgataaa ttacctgtat accaattgta
    15421 aacatacatg ttatagataa aaaatatgtc ccattataac ttcctgttat caatccaata
    15481 ctctcttttg cctgtttaat aatactatct cgtgcttctt tttcacctcc actgccatta
    15541 gttttcacac ataaaaaaac gataaagaca acaaacatca agaagaacga tactatatag
    15601 ccagatttat aagaatcata gaagtagaca actaatattc caacagcacc agttatcaca
    15661 ctcccaaagg aagataccct ttcgcataaa tgagcacata aggtaaactt ttcatttcta
    15721 ccatccagag cacttaacac ccatccctct atagccccag atatacaaca aataccaatg
    15781 gcataaaata tttcagaaaa aaccaatgcc agcatatcag gagactgaat acaaagaggg
    15841 taaaataata tagtaaaaaa tacgccacta actacaacct gtttcctgta aaatctgtct
    15901 gcaatgacag ataaaggaaa atcaagaatc agcactgtca cagaaaaaac gatctgtaaa
    15961 acagcaagtt gagatatagt gacacctgta gatataagaa atgctgcatg gtagacacca
    16021 attaacatac gtatagcatt ataagctcca tagttcacag ataaaatatt tgcagtattc
    16081 acattctatt tccacacaca gagcaaacag gcgaacggcc catattttgg gaatgtattt
    16141 ttatttcatc tgaccagatg cctattcttt tattaagtga taatggctcg gagtattttc
    16201 caatgaactt aattacgtct gccgcacata atgcagcggc aacattattc acaggtgcaa
    16261 atgttgccgg tttaaatctg ctattgatta gcttaatctt atgatcaatg ttttcctttt
    16321 ctgaaccata tagatctgcc acgacttttt ggcactcata acatcctgtt ttaccaggaa
    16381 catataatgg accaaagact gcaatatcat taacataccc agcatttata tagggctgat
    16441 ttgctctaac gcaatattta ttaacccagt taatcagatt aaatggatga tctgcagaaa
    16501 caacccatat atcagcttct ggtaccttat gaagatcagt gtaatcgttt atattcaaag
    16561 cgatttcaga caccgaaatt tcagagtttc tttttaataa ctcgcgtttt ataacttctg
    16621 ttttattttt accgacatca tcctctgaaa ataaaacctg ccttgtcaaa tttgtatttt
    16681 ctatttgatc attatcaatt agtataattt cgccaatacc agacgttgcg agtataacag
    16741 acacatgatt tcctataccc ccacaaccaa gaattactac cttagcattt tttagtttat
    16801 cttgcacaag aacaggatta gcaccataac tttgataatg taaaaagtta cgagagtaac
    16861 gattattctc agtagagtta ttatactcgc caggtataat aaagaaattt tccttaagga
    16921 acctaaagca attagagaaa tctttttcag gtatattggc cgtttccaac gctgcagtat
    16981 aactcgatgg ggttatgaaa caatatgcgg tttttataat attttcccaa agcgctaaat
    17041 tctcaacata ttgctccttt ccgcctccac caactaatcc accgctcccg tatcgagcaa
    17101 ttttgacata gcgacccaat atataatcca taaataaacc tcgcagataa cttttgtatg
    17161 ccaccctgta cagggtggca tttaatggac atttatattt ttccattagt ttgcattacc
    17221 agtacgcatt ttacgcctcc tattactgtg attaattcac aagtgttttt atcactataa
    17281 aaaacaatat tccttataaa aatatacaaa catagattta tttcacaaaa aaagaacagc
    17341 acaatcatat tatttgcgat tctacatttt tattctaaaa tttattaatt tatatattta
    17401 gacataaata accatacaga atataatcat ctgcgtttaa atcattttgt aataaatatg
    17461 ctattatgca ataaaagaat ttgatacatc catcgttcat tacattggaa accaataatc
    17521 ctgattacca gtcacgttga gaagccgctc aatgagcggt ggtgcgctac gtgaaatctg
    17581 agttttttcg ggtgggattg gacacgagaa cttatgagaa agcatcagga aatacaatga
    17641 aaatagagat taattttcga tgaaaggcag ctcgcaatcg ccgcattcaa tattcagtcc
    17701 ggacttctcc cagacagcag caccacaccc cgacactgat atttgacttt gttccttttt
    17761 ggcttgttct caaaagccgc aacaaacaga tcgttatcat tgctcccgtt ctacggtgac
    17821 actgacagca ccgcttcaat atcaatcccg gttcagcatt ttgatgtgcc tgagcaatag
    17881 tttcccgcca ttgttcgatg acggcggcca tctctttttg ttgttcaacc tggacagaga
    17941 agcgatcaaa ccagctcaaa gcaaatttcc cctgaaacag atcctgtgtg acgcgctgga
    18001 acgtaatcag acactcgggg agttcaccgt caaaaaggcg ctggttatag aaatcaaacg
    18061 cctgttgtag ctcgctgtag cgcgttctgt ggctatcgtc atcatcagga ctcctgaaag
    18121 acaacccagc atttcgcgcc gtcatattca ttgccacttt ctgcaataaa cacgttatct
    18181 tcagtatcta ccgctatcac tctggggcgc tcatgctgag gggcgcggtg aatatttttc
    18241 cagcaataca cctctccgtc ccaaatcaga atcacacccg aatcagcgtg ccccgggttg
    18301 tgctgacgcc atcccctggc aatatcaagc ccagggagag catcgagcgc gtaatattgc
    18361 tggcaagaaa ggtaagcagg gggtatccct gctttatgta acagaaatca agcactgccc
    18421 catgagccat gtttcaggca ttgctggtaa aagacactta gaagctcttg ctctgttggc
    18481 ggttcttcgc cagaaattcc aattctggct gggataaata cgttttcgtc tttccctgcc
    18541 agtggccgca attcatcaga ctggcgcata acgttatcca acgcttttgc caactccgct
    18601 ccattgctgg ccggaatact ggtcagaacg gccattccac ctttaatctc gcgacgggag
    18661 ccttttttcc tggcgataca cacaacaaat agttgtattt caggttgcaa ccatgcctgc
    18721 tgaacaaagc cgtcctgata gtgcacttca tgatgtgtga ttttgctgag atctgcacct
    18781 gtctggaaat acattaatgg taatgtcaga ctcaaaacat cctcctgttt atcaaaaaag
    18841 tctcctgcgt aaaccagagg cttattatat taatgttcgt cgccagcaga gctggaagca
    18901 tcttttttga tgataaactc atcaagagag cgaccgcctg ccagtgcttc agcaatcggt
    18961 tttggtgcac gcccacgacc agaccagtat ttcgtttcac cattttcttc aaattgatac
    19021 ttgggcggcg cttttggtaa cgttttttta cgtgtttttg gcgcatcttc ggtaatgcca
    19081 agcagttctt ccggtgagaa accctctccg gcgattaact gaagcagttc ctgacgtttc
    19141 cgttcacgct ctgctcgttc agccgcttca cgttcagcat cttcacgacg ttcttcaata
    19201 acaacgttga gcttttcctg catcgccgtg aatctggcgc cgctcataaa tagtgcttga
    19261 gcatcaacaa aaatgcaaaa aggctgatgt tgtcatggct gaacatacgt acgtctgcga
    19321 ccggaaaccc gatcaatgcg gtaaggacag tgccgtcagg ttcgctacgc gaacggtctg
    19381 gcaggaagcg caagcgcagt cccgcgcaat atatcagaaa gcacctcccg aaatacggga
    19441 gggcttcggc tattcagcta aggttgctct gttcaggttg ctgagtgtag ccttccatgc
    19501 cctgacgggc atcaaaaagc cagcgccgga agttactcat tctttcaagg ttagctttgc
    19561 ttggtttttt gccacgatta ccacttaata tccgccagac tcgtattcct gctttattag
    19621 tccagtgcag aacaatttct cctttgctgt taaaagcaag agaaaagcgt acagcgcctt
    19681 gccgcaattc tccactgaca aaggacacaa aatcatgttt tgtcataaag cctccggttt
    19741 atttatctgt tctcttttgt gtcttttttt ccagtgcttt tctgagtaat gccatatact
    19801 caggaaagac atggaatggc atcaaaggga aaaccggacg cccacccttt tcaacgtcat
    19861 ttattgctga taatatgcgt tccgacattt tattcctcgc aatagaatca ggtgtcacgg
    19921 tatcaggttg ccccacaacc tgataccatc tttatcttta ctttattaac ttctcaattg
    19981 cttctttttt tgctttctcc tgttctaact ttctcctgtt ctgatggtgg gtatgcaagg
    20041 tcataccagt tatcaataca atgaataact ctttctttag ataatccaca ttgctcaaac
    20101 caaaatttta tatttcggtt taatgatgct tttgcgctaa actctctttc cattttcaga
    20161 cctttactaa aagggggaat atcccccctg attaattagt agtaagaaga aaatcgatgt
    20221 ttttcttccg gtagagtttt atacgcagtt tcaaaatctt cgattttgat tgccagctca
    20281 tcaccaaaat cttttatctg acgcttaaaa cattcaccag taaaatcatt taaaacggga
    20341 gttttaaagc cgaccattga atcggtatat tctgaattgg cgcgaatctc gcgaacggtg
    20401 acggtttttt taccgtgaac agaaagaacc tggtagaagg ttacattggt ttgctcatag
    20461 ccccatgatg atacaaagat atcaccaaca gagacagtat catttttaaa agttaatact
    20521 ttcattttta gctcccgccc agcttgggct ctggatgaaa gagaacctgt ttccctttcc
    20581 tgtgttttat tatacctgca tttgcaggta tggcaagtta tttttttacc agcctgtaag
    20641 acggatgcat atttgctaat aacaataaat actgatattc accaatagat aaagaacgac
    20701 tggccttacc tgcactttct ttcatctgcc aggaattcag ttgataacca aaaatatcag
    20761 cagcttcttt aagagtaaga cctgccgcaa cccgcgcatt ctttacttct tcaggtgtag
    20821 gtttattctg cattttcatc cccttttgtc agacaatcac acaaaaccca attatccaga
    20881 agcatcaatg atgccaggtt gagtttttcg tccattatca attttcttac tgttttcctg
    20941 cttttagttt tatcaatcgt cttaacatat tttaagacgt gaaaaaccgt atccccatct
    21001 tcatccatcg ttctgattac ataggagtaa ttacctctgt ttttcttaat tatcttatct
    21061 ttatccatat tattcctcat aactaaaaaa ggggcttaat gccccttttt atctataaca
    21121 cggtatgtaa ccctgtttcc tgtgtaaata aacgcgctaa ctgatcacaa tagatattat
    21181 cgaatcttga aatatcttta acttcagcac cacgtacacg agaaaatttc acctggtacg
    21241 tatcagtaaa atccagttta atttttacta agttgatgcc tttcattgca aaatttgacg
    21301 gcaaacggaa agttaaccca cactcaccgt tttcatcaaa gtaagaaaaa cttttagcac
    21361 cagtcataac aataaaacga ttaccaccaa gttgacgtaa aatctctgtg gctacagatt
    21421 tagcgtagtt ttgcattttt aacctcccgc ccactttggg ctctggatga aagagaacct
    21481 gtttcccttt cttgtgtttt attatacctg catttgcagg tattgcaaat ttttaatgct
    21541 ctcgctctga ctgtaaacag tcaatcattt gatgattaat cttagcgcaa atctgcgatc
    21601 tgatagactt cgctgctttc ccttcttcca acggcaataa cgagtaaaac gactttctca
    21661 tcaatgactt gatatacaag tctgtagcca gatgcacgaa gtttaatttt atagcgatca
    21721 gcatgaccat gtaaccgggc tgcgggtacg cgtggatttt ccagccgttc tgccagtttc
    21781 tttttgaatt gttcacggat ggtgtggccg agtttctgcc attccttcag tgctctacgg
    21841 tcaaaagcca gttcataggt catccaggtt aaccttcaca atttccgggt ttttcatgcg
    21901 ctcatcagca acagcgttta actcagcatc ttcagcgagt tcccgatagt aggcgtaaag
    21961 ttttggtgga acgcaataga acgccggttc gtttcggttc aggatcgcaa cagcgtcccc
    22021 ttcaccttca gctacggttc ccataggatt ttttttcagg tcagtaatac tggccgctgt
    22081 ggttgtcaga atttgatagc tcataagccc tccgcttgtc tcattggcat aatagcatct
    22141 ttaaaagcac tcttaaaagt gcttatgttg agaaaaacat cagaacaggt gagatatggc
    22201 tctttccgac atcgccttaa aacgctctgt aacgcgttct gagcgttttt tcccgtttcg
    22261 ttgtagtcag ctacggttta acggctttta acgccgctca acgcttttta gcggccttgt
    22321 tgctatgatc cggataggag ttcaatcttg atcaaaattt cctcaaggca acgactgttt
    22381 tgtaaatacc ctctaaaacg cgctgtatcg cattctgagc gattttttcg atttcacatg
    22441 gattttctta tggttgtact gaacgaagct gcagaaagcg attcagggct tctcagtgga
    22501 agatccaaaa aggatcaatc aacggatcgt atgatctgaa ctcttctggt tttgagaaag
    22561 gagacaccgc tcgccgcagc cgaacgaccg agcgtagcga gttagtgagc gaggaagcgg
    22621 aacagcacag ggataacatc aggcaattac gcacagtggt taaaaagcga gcatattgtt
    22681 tttatgtgtg gttgtcctgg ttcagagtga tctgtcctct gggtttacgc aaagttatcc
    22741 acaggcactg tgaatttttt gttttgagca gagttatcca cagatttttt gtgttttttt
    22801 gcttattaaa cttcttgttt ttaagatctt aaatgtagat cggtagatct actttaagat
    22861 ctttaactta ctttaactta ttctgcaaac attgtgccag cttttttatc aataatttca
    22921 ttaagatagg tgttgttttt tgtcgcctca aaatgaaagg tggatgggta cgcactgaaa
    22981 ggtggatggg tacgcactgt aattttcatg aaaggtggat ggctgcgcac tgaaaggtgg
    23041 atgggtacgc actgaaaggt ggatgggtac gcattttact gtttttattc agatattcat
    23101 ctctttgcat cttcatttct ttagttcttt atagcgttcc aatccaacgg taaaagttaa
    23161 aaggtggata cctgcgcatt tctatcatat tgaatataaa tgataaaaat tctttcatgt
    23221 gatctgtgtt ttggtggaaa tgtataaggt ggataaacca ctttacacat ttccgctttt
    23281 gtggttatga tttcaccatc aggacttaaa aaacacaggt aatgtatgaa cttactgagc
    23341 gttacaaaga aaacaaaaat tcgccacaga aatgaaatta acagcacatt ctcatcgttg
    23401 cctttgtctg ccagaagaat tttgtttatg gctatggcgc agattgattc acgtgaagag
    23461 ttatctgagg gagcaacctt tcgtattacg gctcgggaat atgcatttat cgctgatatt
    23521 gatgtaacag gtgcttaccg gcagttaaaa gagggggcag aagaacttca ggctagtgtt
    23581 attcgcattc ctcgaaatca acttctcaca gctcagggca atcaaagctc tggacatacg
    23641 aagaagagag gtaaattacc ttctgatgca gtcaggctta tgaacctgac tgcgttttgt
    23701 gattatgttg aaagtgaagg atatattgat attgcttttt cacatgtcat tgagccatat
    23761 atcacgatgt taaaggattc ttacactact caggttttga tatcgtctgt aagactcgct
    23821 gatcaaaatt caaatatgct ttatcagttt atcaggaaac aatacagcag tggtaaaaag
    23881 acatttttcg acattgaagt agatgtatta aaggatgaac ttgaattatt cagaatagat
    23941 aatggcgaaa aagtttacct ttatcagaat tttaaggatt ttaataaggt ttttctgcaa
    24001 aaaaacattg aaaaaataaa tcaatataca gagattaatc atctcgaagt taaaattatt
    24061 gagagagtcg caagaagagc atcaaagcta agattttcgt ataaaataga taaagaaagc
    24121 gaaggacttg ataccagaat accgtatggt ttcagaggtg cttaatatat aaaggtaaat
    24181 acact
//
