LOCUS       NZ_CP117224             2014 bp    DNA     circular CON 23-MAR-2024
DEFINITION  Klebsiella pneumoniae strain ATCC BAA2146 plasmid pATCCBAA2146_1,
            complete sequence.
ACCESSION   NZ_CP117224
VERSION     NZ_CP117224.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32977652
            Assembly: GCF_028596205.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Johnson,J.D., Blankenship,H. and Soehnlen,M.
  TITLE     Long read genome assemblers struggle with small plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Johnson,J.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2023) Bureau of Laboratories, Michigan Department
            of Health and Human Services, 3350 N Martin Luther King Blvd.,
            Lansing, MI 48906, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2022
            Assembly Method                   :: Trycycler v. v0.5.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 178x
            Sequencing Technology             :: Oxford Nanopore, Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028596205.1-RS_2024_03_23
            Annotation Date                   :: 03/23/2024 03:37:23
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,785
            CDSs (total)                      :: 5,662
            Genes (coding)                    :: 5,529
            CDSs (with protein)               :: 5,529
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 133
            CDSs (without protein)            :: 133
            Pseudo Genes (ambiguous residues) :: 0 of 133
            Pseudo Genes (frameshifted)       :: 49 of 133
            Pseudo Genes (incomplete)         :: 79 of 133
            Pseudo Genes (internal stop)      :: 17 of 133
            Pseudo Genes (multiple problems)  :: 12 of 133
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117224.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="ATCC BAA2146"
                     /db_xref="taxon:573"
                     /plasmid="pATCCBAA2146_1"
                     /geo_loc_name="USA"
                     /collection_date="2022"
     gene            770..1249
                     /locus_tag="PQQ24_RS00830"
                     /old_locus_tag="PQQ24_00830"
     CDS             770..1249
                     /locus_tag="PQQ24_RS00830"
                     /old_locus_tag="PQQ24_00830"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_017896843.1"
                     /translation="MRKTLLLCIPFFLLAGCAQTKPQMPDANYEKFAKVEVASDACLNS
                     NFITAQEAGQAHSNISLFLSSWTYDYVRYSNLLAQGHQDVKKIKITQEGCNLLRAKIYQ
                     DTIEAQRYEKQMEIAVQQKAIADQQALQSIQNMQSTSPKTTYCNQIGTQTICNSY"
                     /name="hypothetical protein"
     Typing          160..669
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.743"
                     /coverage="99.0"
                     /evalue="5.94e-177"
ORIGIN
        1 tcattcgctc actgcgttcg gtcatgactc tgcggcgagc gttacgggat caatcaaagg
       61 gcgtggaggt atgaagtgtg agaagagact taagggctgc cggttttcca taggctccgc
      121 ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga
      181 cttaaagata ccaggcgttt ccccctggtg gctccctcgt gcgctctcct gttcctgcct
      241 ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt atctcattcc acgcctgaca
      301 ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag
      361 tccgactgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacacgac
      421 aaaacgccac tggcagcagc cactggtaac aggtttgtgc gagagatgtg ttcagagttc
      481 ttgaagtggc ggcctgactg aggctacact agaaggacag atttggtgac atgtctcgca
      541 caagactgtt accacggtta gagaatctgc caggattaac taaccttcga aaaaccacct
      601 gccagggtgg ttttttcgtt tacagagcaa gagattacgc tctgccataa atgatctcaa
      661 gaagatttag gttattaagt aggacgaaga tcgctatgct ccccgtgatt ttttatatac
      721 caaccacggg gcattagttt tttcttgtca aaaagggata aaaaactcaa tgagaaaaac
      781 attattactt tgcattccgt tttttttact tgctggttgc gctcaaacaa aaccgcaaat
      841 gccagatgct aattacgaaa aattcgcaaa agttgaggtt gcctctgacg cctgcttaaa
      901 ctccaacttc attacagcac aggaagccgg tcaggcacat tccaacatat ccttgttctt
      961 aagttcatgg acatatgact acgttcgtta ctcaaacctc ttggcgcaag gacatcaaga
     1021 tgtaaaaaaa atcaagataa cacaggaagg ctgcaacctt ttgcgcgcaa aaatctatca
     1081 agacaccatc gaggcacaga gatatgaaaa acagatggag atagcagtac aacaaaaagc
     1141 catagcagat cagcaggcgt tgcagtcgat tcaaaatatg caaagcacat cacctaaaac
     1201 aacttactgt aaccaaattg gcactcaaac aatctgcaac agctactgat caaaaactga
     1261 tttaccataa catcccttgc ccgcaccccc tgagaggccc tcagcgcgtt tctgccgcgt
     1321 cctctgcgtt tccggcacct ttccctgtcc atatcgtgaa aaaacgcgcc tgatgatttc
     1381 tgcgcgcctg catggctatg cacagaagtg aatggcggcc ggtttcggac gaatgctggc
     1441 actaaaggcg aattttgaac aaaaaaaacg gacatcgagg gtcaggatgt ccgacaaaat
     1501 ttgcacaaac agtcagccgg taccgatcag agagccggca aataagtatg acagtaaaaa
     1561 gtggttatca actgcccccg cgctgctgat tgcgcagttt tagggctttg gccagtgcag
     1621 gcaggcccgg ccggaatcag gggatctggt aacggtgaac gggtgcacag ttgactgtgc
     1681 ggccgtgagg cgttggccaa gccgccaggt gcggcagctt ccggtgggta caaacgccag
     1741 cactttgcga atcctgccct agatctcaac agcagagaaa tttcgtacgc acgaaactca
     1801 gcgtatcggg cggcgcctga tgaaacattc tggagtgtcg gcggtactcc aaaaacacat
     1861 cttgtcccgc tgattttgtc actggcgcaa agcggcggta atgacaagac atttcctgtc
     1921 cgcatcaata cgtgcagaaa acgtaagtgg tgaaagatga tagtacggag cgacaggagc
     1981 ctaagcagcc accggagaaa ttacgcttcc tcgg
//
