LOCUS       NZ_CP117173             8500 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Serratia proteamaculans strain CD3406 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP117173
VERSION     NZ_CP117173.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17103453
            Assembly: GCF_019295365.2
KEYWORDS    RefSeq.
SOURCE      Serratia proteamaculans
  ORGANISM  Serratia proteamaculans
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Yersiniaceae; Serratia.
REFERENCE   1  (bases 1 to 8500)
  AUTHORS   Begrem,S., Jerome,M., Leroi,F., Delbarre-Ladrat,C., Grovel,O. and
            Passerini,D.
  TITLE     Genomic diversity of Serratia proteamaculans and Serratia
            liquefaciens predominant in seafood products and spoilage potential
            analyses
  JOURNAL   Int J Food Microbiol 354, 109326 (2021)
   PUBMED   34247024
REFERENCE   2  (bases 1 to 8500)
  AUTHORS   Sauvage,T. and Passerini,D.
  TITLE     A comparison of Oxford nanopore library preparation strategies for
            multiplex bacterial genomics
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 8500)
  AUTHORS   Sauvage,T. and Passerini,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2023) EM3B, IFREMER, rue de l'ile d'yeu, Nantes
            44311, France
REFERENCE   4  (bases 1 to 8500)
  AUTHORS   Begrem,S., Passerini,D. and Leroi,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2020) EM3B, IFREMER, rue de l'Ile d'Yeu, Nantes
            44311, France
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 03-FEB-2023
            Assembly Method                   :: Unicycler v. V0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 134.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_019295365.2-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 07:42:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,618
            CDSs (total)                      :: 5,500
            Genes (coding)                    :: 5,443
            CDSs (with protein)               :: 5,443
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 57
            CDSs (without protein)            :: 57
            Pseudo Genes (ambiguous residues) :: 0 of 57
            Pseudo Genes (frameshifted)       :: 16 of 57
            Pseudo Genes (incomplete)         :: 43 of 57
            Pseudo Genes (internal stop)      :: 12 of 57
            Pseudo Genes (multiple problems)  :: 9 of 57
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117173.1.
            Bacteria and source DNA available from Collection of Ifremer_EM3B
            laboratory.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8500
                     /organism="Serratia proteamaculans"
                     /mol_type="genomic DNA"
                     /strain="CD3406"
                     /isolation_source="shrimp seafood product"
                     /db_xref="taxon:28151"
                     /plasmid="unnamed5"
                     /geo_loc_name="France"
                     /collection_date="2006"
     gene            1..951
                     /locus_tag="JET59_RS27985"
                     /old_locus_tag="JET59_027985"
     CDS             1..951
                     /locus_tag="JET59_RS27985"
                     /old_locus_tag="JET59_027985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946646.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_034167667.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSHLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
                     /name="replication initiation protein"
     gene            959..1372
                     /locus_tag="JET59_RS27990"
                     /old_locus_tag="JET59_027990"
     CDS             959..1372
                     /locus_tag="JET59_RS27990"
                     /old_locus_tag="JET59_027990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001229901.1"
                     /translation="MALVSISEASRLTNKSRTTVHRYISKGKLSICTDENGVKKIDTSE
                     LLRVFGAFKAVHHEQIDNVTDEQHVTPNRTGETSKTKQLEHEIEHLRQLVTAQQSHIDS
                     LKQAMLLIESKLPTTREPVTPPVGKKSWQFWKK"
                     /name="hypothetical protein"
     gene            complement(1735..3201)
                     /locus_tag="JET59_RS27995"
                     /old_locus_tag="JET59_027995"
     CDS             complement(1735..3201)
                     /locus_tag="JET59_RS27995"
                     /old_locus_tag="JET59_027995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008853313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="WP_221344862.1"
                     /translation="MATDSNGHQQVGSITHEVYIVLRHNVKWVSLSQCFKISCQLLGLV
                     VFARYLTPEEIGVMSLTLVVVNFVNILRDMGSSAAIIQRVYIDDRLKNSVFVFNVLIGC
                     FLFFIFYFLSNDISSFFKKVELAGTIKAISFAFIFNSVTSVHLALLERASKFKVVAIIE
                     SFSSIISLLFGIALAIKGFGVYSLVGQTLMYSLLTSIMFHIFSGWKPAFTFDFRDIKSI
                     FNFSSNIVAFNFVNFFSRNSDQIIIGKFFSASILGQYSMAYKVMLFPIQSVTAVLTRSL
                     YPVLSRLQESKQDALSAYLNVLKVISLVVTPMMLGLSSVSHELVNIAFGKQWVLLPNLL
                     TWLSIVGILQAMVSTTGSVFMSQGDARTLFIISVFNSILQVGSFIIGVNYNIETLVILY
                     MIANFIMFLPNMYLSVKTLNGSILDVLRCVYKPVFSGVIMFISIKVLSCFSLHFSNVSF
                     LLIKIGFGFLVYTSLILFLDRKLVKMIFKM"
                     /name="lipopolysaccharide biosynthesis protein"
     gene            complement(3323..4150)
                     /locus_tag="JET59_RS28000"
                     /old_locus_tag="JET59_028000"
     CDS             complement(3323..4150)
                     /locus_tag="JET59_RS28000"
                     /old_locus_tag="JET59_028000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="WP_117074822.1"
                     /translation="MISVNLTTMHSRLDLCAATVWSIINQSVQPDKINLWISNEPYLSD
                     HGIYEIPAWVEELNKINNIVNVHYVKNTGPYRKIINAIINSEIDDILVYADDDVIYGRQ
                     WLEFLLGQFYENNCNVAIAARIRIMERNIFNKYKSYSSYSISMKKDKFEKDFIITGVGG
                     CVLSKRMIKPSIIGDDAYLIISPKTDDIWISKMLEASNTKVESSPDALRCVHEILHDHN
                     TLSSINTLTSKNSGLIIKAIRMVKNKFYSYFGLQKTNNDKSIKMVNLHFNGKL"
                     /name="glycosyltransferase family A protein"
     gene            complement(4415..5383)
                     /locus_tag="JET59_RS28005"
                     /old_locus_tag="JET59_028005"
     CDS             complement(4415..5383)
                     /locus_tag="JET59_RS28005"
                     /old_locus_tag="JET59_028005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS903B family transposase"
                     /protein_id="WP_013087110.1"
                     /translation="MKDQITYLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLDD
                     EAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVPLR
                     CPDYSCVSRRAKSVNVSFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRIWRK
                     LHLAVDSKTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRSAAADGAYDTRLCHDELRR
                     KKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETAMYRVK
                     QLFGGSLTLRDYDGQVAEAMALVRALNKMTKAGIPESVRIA"
                     /name="IS5-like element IS903B family transposase"
     gene            complement(5580..6164)
                     /locus_tag="JET59_RS28010"
                     /old_locus_tag="JET59_028010"
     CDS             complement(5580..6164)
                     /locus_tag="JET59_RS28010"
                     /old_locus_tag="JET59_028010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WP_040113348.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVTSLTEQFQDASAQLQQQSDTLSR
                     LIRELDSASGNMTETVRQSVHAALIQVEKELKQAGLAQQKPATEALNQATDTAKAMIHE
                     MRREMSRYTWKSAIYLVLTIFFVMAACVTAFTWFMNDGYSQIAEMQRMEAVWQKKAPLA
                     DISRCDGKPCVKVDTRATYGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     gene            complement(6177..7274)
                     /gene="mobA"
                     /locus_tag="JET59_RS28015"
                     /old_locus_tag="JET59_028015"
     CDS             complement(6177..7274)
                     /gene="mobA"
                     /locus_tag="JET59_RS28015"
                     /old_locus_tag="JET59_028015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_040113349.1"
                     /translation="MASYHLSVKTGGKGSASPHADYISREGKYAREKDSDLEHKESGNM
                     PAWAAHKPTEFWKAADTFERANGCTYREIEIALPRELKPEQRLELVRDFVRQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERMNDGIDRDPQQYFKRANSKAPERGGAKKARFG
                     ETPTERKEHLVAQRERWADLQNKHLERYQHTDRVDARSLKAQGIEREPERHLGAGQVQR
                     FDTAQLQAILERRDAERQAEQSRDERDSVIDVTTSLREAISERDNLTIKQEQKPEPAQE
                     DTSGRVFDFEKEPEKLNALVSDALKDVQDEIDLQSLVNETMAEFQGIHQEMERLAEKQR
                     QQEKEQQRLAEQTRQKPDKGWSFSR"
                     /name="mobA"
     gene            7440..7907
                     /gene="traD"
                     /locus_tag="JET59_RS28020"
                     /old_locus_tag="JET59_028020"
     CDS             7440..7907
                     /gene="traD"
                     /locus_tag="JET59_RS28020"
                     /old_locus_tag="JET59_028020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_032235402.1"
                     /translation="MSKSEKQEQWAAERVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLILAGLV
                     DSKTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSTT"
                     /name="traD"
     Typing          776..951
                     /name="ColE10"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          complement(6181..7274)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtctgaat tagttgtttt caaagcaaat gaactagcga ttagtcgcta tgacttaacg
       61 gagcatgaaa ccaagctaat tttatgctgt gtggcactac tcaaccccac gattgaaaac
      121 cctacaagga aagaacggac ggtatcgttc acttataacc aatacgctca gatgatgaac
      181 atcagtaggg aaaatgctta tggtgtatta gctaaagcaa ccagagagct gatgacgaga
      241 actgtggaaa tcaggaatcc tttggttaaa ggctttgaga ttttccagtg gacaaactat
      301 gccaagttct caagcgaaaa attagaatta gtttttagtg aagagatatt gccttatctt
      361 ttccagttaa aaaaattcat aaaatataat ctggaacatg ttaagtcttt tgaaaacaaa
      421 tactctatga ggatttatga gtggttatta aaagaactaa cacaaaagaa aactcacaag
      481 gcaaatatag agattagcct tgatgaattt aagttcatgt taatgcttga aaataactac
      541 catgagttta aaaggcttaa ccaatgggtt ttgaaaccaa taagtaaaga tttaaacact
      601 tacagcaata tgaaattggt ggttgataag cgaggccgcc cgactgatac gttgattttc
      661 caagttgaac tagatagaca aatggatctc gtaaccgaac ttgagaacaa ccagataaaa
      721 atgaatggtg acaaaatacc aacaaccatt acatcagatt cccacctaca taacggacta
      781 agaaaaacac tacacgatgc tttaactgca aaaattcagc tcaccagttt tgaggcaaaa
      841 tttttgagtg acatgcaaag taagtatgat ctcaatggtt cgttctcatg gctcacgcaa
      901 aaacaacgaa ccacactaga gaacatactg gctaaatacg gaaggatctg aggttcttat
      961 ggctcttgta tctatcagtg aagcatcaag actaacaaac aaaagtagaa caactgttca
     1021 ccgttacata tcaaagggaa aactgtccat atgcacagat gaaaacggtg taaaaaagat
     1081 agatacatca gagcttttac gagtttttgg tgcattcaaa gctgttcacc atgaacagat
     1141 cgacaatgta acagatgaac agcatgtaac acctaataga acaggtgaaa ccagtaaaac
     1201 aaagcaacta gaacatgaaa ttgaacacct gagacaactt gttacagctc aacagtcaca
     1261 catagacagc ctgaaacagg cgatgctgct tatcgaatca aagctgccga caacacggga
     1321 gccagtgacg cctcccgtgg ggaaaaaatc atggcaattc tggaagaaat agcgctttca
     1381 gccggcaaac cggctgaagc cggatctgcg attctgataa caaactagca acaccagaac
     1441 agcccgtttg cgggcagcaa aacccgtact tttggacgtt ccggcgggtt tttgtggcga
     1501 gtggtgttcg ggcggtgcgc gcaagaagac gttatggtaa ataagtattt tttttgataa
     1561 aaatacttat tttcataaaa gacgctcttc tatcttcaag cgggtaatga tagctgcttg
     1621 ctgattccac attttcgctg ccgccaaggt tacccagagt gacaaggatt gaatgacttt
     1681 agattcaaat agtcattaac aggttggcag ggtgatgctg ccaactagta attgttacat
     1741 tttaaatatc atcttcacta actttctatc caaaaaaaga ataagtgacg tatagacaag
     1801 aaaaccaaaa cctattttga taagtaaaaa actcacattg gaaaaatgta aagaaaagca
     1861 agacagaacc tttattgaaa taaacataat aacccctgaa aaaacaggct tgtaaacaca
     1921 acgcaacaca tcaagaatgc taccattaag agtttttacg gataaataca tattaggtaa
     1981 aaacataatg aaattagcta tcatgtataa aataaccagt gtttctatat tgtaattaac
     2041 accaataata aatgagccaa cctgcaaaat agagttaaat actgatatta taaataatgt
     2101 tctagcatcc ccttgagaca taaatacaga acctgtagtg cttaccattg cctgtaaaat
     2161 acctactatg gataaccacg tcagtaaatt tggcaggagc acccattgtt taccaaaagc
     2221 gatattaact aattcatgac taactgatga aagaccaagc atcataggag taacaactaa
     2281 tgaaattact tttaatacat taaggtatgc tgataatgca tcttgttttg attcctgcaa
     2341 gcgactaagc acaggataca atgatcttgt taaaaccgca gttacgcttt gaattgggaa
     2401 tagcattact ttataagcca ttgaatattg cccaagtatt gaagctgaaa aaaacttacc
     2461 tattattatt tgatcactgt ttctagaaaa aaaattcaca aaattaaatg cgacaatatt
     2521 tgaactaaaa ttaaatattg actttatatc cctaaagtca aatgtaaatg ccggtttcca
     2581 tcctgagaat atatgaaaca taattgatgt cagcagagaa tacatcaatg tttgcccaac
     2641 taaactatat acaccaaaac ctttaattgc taatgcaatg ccaaaaagaa gtgaaataat
     2701 tgatgagaat gattctataa tcgcaacaac tttgaatttt gacgccctct ccagtaatgc
     2761 aaggtgcact gacgttacac tattaaatat aaatgcaaag gatattgctt ttattgtgcc
     2821 tgccaactcc actttcttaa aaaaactaga tatatcatta gataaaaaat aaaaaataaa
     2881 aaataaaaaa caaccaatga gcacattaaa tacaaaaaca gaatttttca acctatcgtc
     2941 aatatacact ctctgtataa ttgccgctga agaccccata tcgcgtaata tatttacaaa
     3001 gttcacaacg acaagagtca acgacataac cccaatctct tcaggtgtta ggtagcgagc
     3061 aaaaacaacc aaacctaaaa gctgacaaga tatcttaaag cactgagaca gtgataccca
     3121 tttcacattg tgtcttaaca ctatataaac ctcatgagta atgctgccaa cctgttggtg
     3181 accatttgaa tctgtagcca ttcaatcctt gtcactctgg gtaaccttgg cgtcagtgaa
     3241 aatgcgaaat caacaaattg tcatcattac caacttactg atgacatttt gttttcagac
     3301 aatccctgat gagaaagatg atttataatt taccattaaa atgcaaatta accatcttga
     3361 tggatttgtc attgtttgtt ttttgcaacc caaaatatga atagaatttg tttttaacca
     3421 tcctgattgc ctttataatt aaaccgctat ttttagatgt caatgtattt attgagctaa
     3481 gtgtgttatg gtcatgcaat atttcatgaa cacatcgcag tgcatctggg gatgactcga
     3541 ccttcgtatt ggaagcctct aacattttac taatccagat gtcatctgtc ttaggtgaaa
     3601 taattaaata tgcatcatct ccgataatac taggcttaat cattctctta cttaaaacac
     3661 aacccccaac gccagttatt ataaaatcct tttcaaattt atcctttttc atactaatag
     3721 aataagagct ataagatttg tatttgttaa aaatattcct ctccattatt ctaattctag
     3781 cggcaattgc cacattacag ttgttctcat aaaactggcc taataggaac tcaagccact
     3841 gccgaccata tattacatca tcatcagcat atacaagaat gtcatcaatc tcagagttaa
     3901 taattgcgtt aattattttg cggtatgggc cggtgttttt tacataatgt acattcacta
     3961 tgttatttat tttatttagc tcttcaaccc aagcaggtat ctcatatatt ccatgatctg
     4021 acaaatatgg ctcatttgat atccaaaggt ttattttatc tggctgaaca ctctggttta
     4081 taattgacca cactgtggcg gcacataaat ccagtcgaga atgcattgta gttaaattaa
     4141 ctgatatcat gctacccttt aaagaaaaaa attaatttga ttttatatta tatgtaaaaa
     4201 ggttacatac acaatatgta ggacgttttt tcgaaaaaaa tcagtttttt tcctgatgaa
     4261 ccttcctccg cgttgcccgg tgatgctgcc aacttactga tttagtgtat gatggtgttt
     4321 ttgaggtgct ccagtggctt ctgtttctat cagctgtcgg ctttgttgaa taaatcagat
     4381 ttcgggtaag tctcccccgt agcgggttgt gttttcaggc aatacgcacg ctttcaggta
     4441 tacctgcttt cgtcattttg ttcagcgctc gtaccagggc catagcctcc gcaacctgac
     4501 catcgtagtc acgcagcgtc agtgaacccc cgaacagctg ttttacccgg tacatcgccg
     4561 tttccgctat cgagcgacgg ttgtaatctg ttgtccattt ccaccgcgca ttactcccgg
     4621 tcattcgctg attagccact gcacggttac ggtctgcata ttcaccgggc cagtaacccg
     4681 caccttttcg gggagggata agcgcgctga ttttcttacg ccgcagttca tcgtgacata
     4741 gccgggtatc gtaagcgcca tcggcggcgg ctgacctgat tttccggtgg gtttgccgga
     4801 ttaacccggg gaaggcctct gagtccgtaa cgttgttcag cgacaggtca gcgcagatga
     4861 tttcatgtgt tttactgtca acggcgagat gcagcttacg ccagatacgg cggcgttcct
     4921 ggccatgctt tttgactttc cactcgcctt caccgaagac cttcagcccg gtggaatcaa
     4981 ttaccaggtg tgcgatttca ccccgggtgg gcgttttgaa actgacatta accgactttg
     5041 cccgcctgct gacacagctg taatccgggc agcgtagcgg aacgttcatc agagaaaaaa
     5101 tggaatcaat aaagccctgc gcagcgcgca gggtcagcct gaatacgcgt ttaatgacca
     5161 gcacagtagt gatggcaagg tcagaatagc gctgaggtct gcctcgtgaa gaaggtgttg
     5221 ctgactcata ccaggcctga atagcttcat catccagcca gaaagttatg gagccacggt
     5281 tgatgagggc tttattgtag gtgggccagt tggtgatttt gaacttttgc tttgccacgg
     5341 aacggtctgc gttgtcggga agatacgtga tctgatcctt caactcagca aaagttcgat
     5401 ttattcaaca aagccccgat taagacaaaa tcccctgaaa acgctgtata gcgcgggagt
     5461 acaccctatt gctataggta agtctgttca aaaaacaggc ttaccgtaca ataattctct
     5521 atatccaaac tgacccccta attcacgaca acaataacgg cgcaatgtgc gccgttatgt
     5581 tattttttga taatcatcca ggtattctct ttatcaccat acgtagctcg ggtgtccacc
     5641 ttaacgcagg gctttccgtc acaacgcgat atatcagcca gcggggcttt cttctgccag
     5701 accgcttcca tccgttgcat ttcagcaatc tggctgtacc cgtcattcat aaaccacgta
     5761 aatgccgtca cgcaggcagc catgacgaaa aatatcgtca gcacgagata aatcgcggat
     5821 ttccacgtat agcgcgacat ctcgcgtcgc atttcatgga tcatcgcctt cgccgtatcg
     5881 gtagcctgat taagcgcttc tgtcgccggt ttctgctgtg ctaatccggc ttgtttcagc
     5941 tctttctcaa cctgaatgag cgcggcatga acagattgcc tgacggtttc agtcatatta
     6001 ccggacgcgc tgtccagctc acgaatgagc ctgctcagcg tgtcactctg ctgctgtaat
     6061 tgtgctgagg cgtcctgaaa ctgctcagtc agagaggtaa cacgcttttc cagcgcctga
     6121 tgatgcccga tgagggcagc gatttgttta atttcgtcgc tcatgaaaaa tcctgtctat
     6181 cgtgaaaatg accagccttt atccggcttc tgtcgtgtct gttcggcgag tctctgctgt
     6241 tctttctcct gctgacgctg tttttctgcc agtcgctcca tttcctgatg tatcccctga
     6301 aactccgcca ttgtctcgtt cacaagggac tgaaggtcga tttcgtcctg tacgtccttc
     6361 agcgcatcac tgaccagtgc gttcagcttc tccggctctt tctcaaaatc gaaaaccctg
     6421 ccggaagtgt cttcctgcgc aggttcaggc ttctgctctt gttttatcgt caggttatcc
     6481 cgctcgctga tggcttcccg tagcgaggtg gtcacgtcaa tcacactgtc acgttcatca
     6541 cgagactgct ccgcctgcct ttcagcgtcc ctgcgctcaa ggatggcctg taactgcgca
     6601 gtatcgaacc gctgaacctg tcccgcgcca agatgccgct caggctcacg ctcaatgccc
     6661 tgagctttga gtgagcgtgc atcaacgcgg tcggtatgct gataccgttc aaggtgctta
     6721 ttctgtaggt cagcccagcg ttcccgctgg gcgacgaggt gctctttgcg ttctgtcggc
     6781 gtttccccga aacgtgcctt tttcgcaccg ccgcgctcag gggctttgct gttagctcgc
     6841 ttaaaatact gctgcgggtc acggtcgata ccgtcattca tccgctcaga gaacatgata
     6901 tgcgcgtgcg gctgctcgcc gccggctatc gccgctttcg gattatgaat ggcgaactga
     6961 tacgcatgac ggtcgccgat ttcctgccgg acaaaatcgc ggaccagttc aaggcgttgt
     7021 tcgggtttca gctcacgcgg cagggcaatc tcgatttcac ggtaggtaca gccgttggca
     7081 cgttcaaacg tgtcagcggc tttccagaac tccgtcggtt tatgcgctgc ccaggcaggc
     7141 atattgccgg actccttgtg ctcaaggtcg gagtcttttt cacgggcata ctttccctca
     7201 cgcgaaatat aatcggcatg gggagaggca ctgcctttcc cgccggtttt tacgctgagg
     7261 tgataggagg ccatcgtgtt tttatcccgc tgaaggcgcg caccgtttct gaacgaagtg
     7321 aagaaacgtc taagtgcgct ctgataaata aaagagttat cagggattgt agttgaattt
     7381 gacctccact gccatcaatg agcataattc ccccgttaaa actcagaggg taaacgggca
     7441 tgagtaaaag cgaaaaacag gaacaatggg cagcagaaag agtgcagtat attcgcggct
     7501 taaagtcgcc gaatgagcaa cagaaactta tgctgatact gacggataaa gcagataaaa
     7561 cagcacagga tatcaaaacg ctgtccctgc tgatgaaggc tgaacaggca gcagagaaag
     7621 cgcaggaagc cagagcgaaa gtcatgaacc tgatacaggc agaaaagcga gccgaagcca
     7681 gagccgcccg taaagcccgt gaccatgctc tgtaccagtc tgccggattg cttatcctgg
     7741 cgggtctggt tgacagtaag acgggtaagc ctgttgatga taccgctgcc ttactgggtg
     7801 cattagccag tctgaatgac ctgtcacggg ataatccgaa gtggtcagac tggaaaatca
     7861 gagggcagga actgctgaac agcaaaaagt cagatagcac cacatagcag acccgccata
     7921 aaacgccctg agaagcccat gacgggcttt tcttgtatta tgggtagttt ccttgcatga
     7981 atccataaaa ggcgcctgta gtgccattta cccccattca ctgccagagc cgtgagcgca
     8041 gcgaactgaa tgtcacgaaa aagacagcga ctcaggtgcc tgatggtcgg agacaaaagg
     8101 aatattcagc gatttgcccg agcttgcgag ggtgctactt aagcctttag ggttttaagg
     8161 tctgttttgt agaggagcaa acagcgtttg cgacatcctt ttgtaatact gcggaactga
     8221 ctaaagtagt gagttataca cagggctggg atctattctt tttatctttt tttattcttt
     8281 ctttattcta taaattataa ccacttgaat ataaacaaaa aaaacacaca aaggtctagc
     8341 ggaatttaca gagggtctag cagaatttac aagttttcca gcaaaggtct agcagaattt
     8401 acagataccc acaactcaaa ggaaaaggac tagtaattat cattgactag cccatctcaa
     8461 ttggtatagt gattaaaatc acctagacca attgagatgt
//
