LOCUS       NZ_CP117048             4074 bp    DNA     linear   CON 11-MAR-2024
DEFINITION  Escherichia coli strain MLI106K2 plasmid p4_MLI106-2, complete
            sequence.
ACCESSION   NZ_CP117048
VERSION     NZ_CP117048.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29553887
            Assembly: GCF_024331495.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Riediger,M. and Zautner,A.E.
  TITLE     Molecular epidemiology of Escherichia coli with resistance against
            third-generation cephalosporins isolated from stool samples of
            European soldiers with diarrhea on deployment in Western African
            Mali
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Riediger,M. and Zautner,A.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2023) Institute of Medical Microbiology and
            Hospital Hygiene, Medical Faculty, Otto-von-Guericke University
            Magdeburg, Leipziger Strasze 44, Magdeburg, Saxony-Anhalt 39120,
            Germany
REFERENCE   3  (bases 1 to 4074)
  AUTHORS   Zautner,A.E. and Riediger,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JUL-2022) Institute of Medical Microbiology and
            Hospital Hygiene, Medical Faculty, Otto-von-Guericke University
            Magdeburg, Leipziger Strasze 44, Magdeburg, Saxony-Anhalt 39120,
            Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-JAN-2023
            Assembly Method :: trycycler v. 0.5.3; unicycler v. 0.5.0; skesa v.
            2.4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/11/2024 05:24:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,054
            CDSs (total)                      :: 4,931
            Genes (coding)                    :: 4,663
            CDSs (with protein)               :: 4,663
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 79 of 268
            Pseudo Genes (incomplete)         :: 196 of 268
            Pseudo Genes (internal stop)      :: 49 of 268
            Pseudo Genes (multiple problems)  :: 56 of 268
            Pseudo Genes (short protein)      :: 4 of 268
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117048.1.
            Bacteria and source DNA available from Institute of Medical
            Microbiology and Hospital Hygiene Medical Faculty Otto-von-Guericke
            University Magdeburg 39120 Magdeburg, Germany.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MLI106K2"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p4_MLI106-2"
                     /geo_loc_name="Mali: Koulikoro"
                     /lat_lon="12.52 N 7.34 W"
                     /collection_date="2014-06-24"
                     /collected_by="Bundeswehr Medical Service"
     gene            73..765
                     /locus_tag="NL423_RS01095"
                     /old_locus_tag="NL423_001100"
     CDS             73..765
                     /locus_tag="NL423_RS01095"
                     /old_locus_tag="NL423_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_023308214.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAELQKT
                     MTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKNDEKREEFRWIQYRAQYAKG
                     EAKARITFSDAVMPYLTQLKGQFTRVVIKNISGLSSSYSIRIYEFLQQFRSTGERIIAL
                     NDFRSMLGIENKYKQFRDLNKILIKPCVEELNKKSDLAVTVETIKKGRTVVALHFRFKE
                     DKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1023..3182)
                     /gene="mobQ"
                     /locus_tag="NL423_RS01100"
                     /old_locus_tag="NL423_001105"
     CDS             complement(1023..3182)
                     /gene="mobQ"
                     /locus_tag="NL423_RS01100"
                     /old_locus_tag="NL423_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_252324707.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALENAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            3361..3615
                     /gene="mobC"
                     /locus_tag="NL423_RS01105"
                     /old_locus_tag="NL423_001110"
     CDS             3361..3615
                     /gene="mobC"
                     /locus_tag="NL423_RS01105"
                     /old_locus_tag="NL423_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091747.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(2825..3183)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.056"
                     /coverage="100.0"
                     /evalue="3.94e-177"
     Typing          3184..3360
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.438"
                     /coverage="100.0"
                     /evalue="2.610000000000001e-86"
     Typing          74..765
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.815"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1027..3269)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.198"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaaaaaaat attccacatt tcttatttgt ggggcacatt aaagcatcac cacatttgaa
       61 cgggatcttt gtatgtcgga cttgattgcc tacaagtcga atgctttggt tgaggccagc
      121 tacaagttga ccctacagga acaacgtttt ctgttgctgt gtatcagtcg attgaaatct
      181 ggtgctgatg ctgaattgca aaagacaatg accattaccg ctgcggaata ttttgattca
      241 ttccctgaca tggggcggaa aaatgccgag gttcagttac aggaagcgat cgatcgttta
      301 tgggacaggt cgatcattct caagaatgac gagaagcgtg aggagttccg ctggatccag
      361 tatcgggcac agtacgctaa aggtgaggcc aaggctcgaa taaccttttc tgacgcggta
      421 atgccttatc tgacgcaact gaaggggcaa ttcacgcgcg ttgttataaa aaacatatca
      481 ggtttatcaa gtagttacag tataagaata tacgagttcc tccagcaatt ccgcagtacg
      541 ggagagagaa ttattgctct taatgacttt cgttcaatgt tagggattga aaataaatac
      601 aaacaattca gagacttaaa taaaatacta ataaaaccct gtgttgaaga actgaacaaa
      661 aaaagcgacc tggctgtaac ggttgagacg atcaaaaagg ggcgcacggt tgtagccctg
      721 cattttcgct tcaaggaaga caaacagatc aagatgacga tctaaaatcg attttaaggc
      781 acctaaaacg ctccctgctg cgtttttttt gtgttagtag tgcgaaggta ctattgaagt
      841 gaaaaattgc gtcaaaagcg ctctaatgca gcgaaaaaag aacccccaat ttttgggggc
      901 ggaaaaacaa aactatgctt gcattatgtg tgtgtgcttc ttgcattcat ccctgtcgat
      961 gaacatcgaa gaacatcgac gaacataaca cgatgttaaa caaaacaaaa tattgttcac
     1021 attcactgtg accaaccgcg atcatgtctc ctctcggtgg tgtgtgttct tgcatcttcc
     1081 tcgcgccgcc attcatccca ggtacgattg aaacccagag agcggaacag atgatccggc
     1141 tgtttttctg gtgtgttgac ggcagcaaaa agcggtgcct cacgggtttt cagatgaaac
     1201 tccataagtt ccggcgcagg tgatatgtgg ctgtctgcat cgtgatttac ggcaacacca
     1261 taccctgcat accgtaaccc cttatcggtc agtgcactga tgaccagctg gctgttatcg
     1321 gctgagggaa cggcaaacgc gggatacagc gtaccattaa gccggacaac accgcccaga
     1381 gagtcatcca cgcccatgac cagcaaaagc tgaagctggc gggtttcccg gtcgacagaa
     1441 ctcattaccg gcatcagcgt cggtagcaga cgtccctgcg taaaatccac cttccttgct
     1501 gccagtgatg gcatggctgg ctggcggtct tctccaagac agaaagactc cggccatgtc
     1561 ttcggctggc ggagattgcg ggtatattgc ggccatgtct ggcaaacctg cgtcagaagt
     1621 tgctcaaagg tcgcagagga ataaacacgt tgcagtggtg gatgttgacc gatttcgctt
     1681 cggtcacaga tataaccact cctgtcacgc attaaatcaa cacgctcaaa gattctggtg
     1741 cggtatttct ccagctcgtt cccccagagc gtgtattctc cgttccggtt gtgaagcaga
     1801 aggtaacagg tcatttcccc gttaccaggc atgggggcct cgccataccc cgtaacgact
     1861 gcgctcagta cccctgaatc cataacggca ttgtcatttt tcctgagctg ttccgctcgt
     1921 cgctccgcgc gactacgttc tgtatcctcc tgctccttcc ggcgcaattc ctcttcacgt
     1981 ttaagcaact ggactctgag agctgcaagg ccggatgccc cccactgctg ccactgtttt
     2041 tcaaatgccg tctgactcgc gggaatgtcc tgtttctcca ccttccggta atgctgtcgc
     2101 aatttttcca tttgctggct cagcgactgg atttttccct gttcgctacg gtattccggt
     2161 gccgcccatt gcatcagggg aatacgggaa aaaccggacg aaaacggcga tgattttttc
     2221 aggctggcag catggctcat cgcttccttt cgttcggtgt tcagctcttc ccagcgtctg
     2281 cgcgtcgcct gcatatctgc catcatcgac atggcattac tggtgagttt ccatgccgca
     2341 ttactgtcct tcggcaacgc ttcgatgatg tcacgtagtg tgtacgaagt tgcgttgtca
     2401 ggaagcacga tattcagaag gtcagccaca cggcgggcag gctctgtcag cccgctgttc
     2461 ctgagttcag tgcgggaaat ctgtatttcc aggttaagcc tgttaatcag acgattctgt
     2521 tcgccgcgtt ctgtttcgat accgcgtgtt tccatcgcac aggcagactt tccgaggtga
     2581 ataccaggcg ttttctcaag cccctgtcgc tcgtatgaac gatggtctat ctcttcctga
     2641 taaccggcgt tctccagtgc tctgtttgca tggtcagccc atgatgcacg ccacgtctcg
     2701 gcatgtgtcc ggtcgttcca gtccctgacc tttccgccga acccagcagg tccgacagcc
     2761 ctcgtggtca gcatgatgtg cgcatgggga tttgttttat ccatgtgatg gaacgccaca
     2821 tcagcaatca tgccctgact gacaaagttt tctctgacaa aggcgagaac ggtttcccgt
     2881 gcggcatcct gcggcagttc acgggggata gcgagttcaa tttcccgcgc aagttgtgag
     2941 ttcttccggc attcggcctt ctcaacggca ttccagagtt cggcacggtt cgtacaccag
     3001 gcaggggctg ttgcgggggt cagaatcaca gcttcagcca caccgctttt gcgggtgtag
     3061 tcgtgagtca gtccggtacg ctcatcaagc atcagtgaac cggaacgata ggcagcggaa
     3121 gctacggcgc tgtagccgct gcttctggag ataattttca tgctgagatg ataaatcgcc
     3181 atttctgccg taatgccttt ttgtagtttt ctttcagccc cgcagggcgc acacaatcga
     3241 aacggagtgg agattgtata agtgcgcact ttgtgatttg tcgtgcatta tgccagctca
     3301 gtttttaaac taccagtgac gcgctaaaat gcgatttttc atcgcgcacg gagttacatc
     3361 atgagcagaa cgctcgaaca gaagattgct gatgctgagg cgagattaca gcgcctgaag
     3421 gcgaagagcc ggagcctgga cacagcgcag aaggttgttg tgggtgcagc attgctggca
     3481 aaggtcagaa agccggagga ggtgcagttg cgtgcctggt tactccagtt cctgaaggca
     3541 gaggtgacac gacaggctga tgtgacgcgc atactgccgc tgattaacga gctggaggcg
     3601 ctgccaggac agtgattgta tcgtccgctc gccgcagccc cagcgaccga gcgttagcga
     3661 gcgagtgggc gaggaagcgg aagtgaatcc gtcgccaggt tgagcacata cgcacggttt
     3721 ttcggtaggt gatatgcgct gaacccgcat gaatactgga tttccacagg gttggaaggg
     3781 gagtattcgg aacttgcaat tctgctttct tgcaacacat tgtttttatt ggttttttgt
     3841 tttcagcgcc ccaagttatc cacaggctcg ccgttaagtt tttagatctt gtttttaaga
     3901 tcttgttttt aatacttgtt ttagggtcaa aattcattaa taaaatcaat atgttatagc
     3961 ggtatatgtg gaggaattta aggtaaaggt ggaggaattt aaggtgttta ggtggaggaa
     4021 tttaaggtaa aggtggagga atttaaggtg ttattcacag aaatgtgagg atat
//
