LOCUS       NZ_CP117036             6659 bp    DNA     circular CON 25-JUN-2023
DEFINITION  Salmonella enterica strain PIW95_S14_0299 plasmid ColRNAI, complete
            sequence.
ACCESSION   NZ_CP117036
VERSION     NZ_CP117036.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32929940
            Assembly: GCF_028551175.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica
  ORGANISM  Salmonella enterica
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Salmonella.
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Zajac,M., Iwan,E., Skarzynska,M., Kwit,R., Skora,M., Lalak,A.,
            Smialowska-Weglinska,A., Kaminska,E., Pietruk,M. and Wasyl,D.
  TITLE     The first description of the complete genome sequence of
            multidrug-resistant Salmonella enterica serovar monophasic
            Typhimurium (1,4,[5],12:i:-) isolate with the mcr-1.1 gene on IncHI2
            found in pig in Poland
  JOURNAL   J Glob Antimicrob Resist 33, 218-220 (2023)
   PUBMED   37086889
REFERENCE   2  (bases 1 to 6659)
  AUTHORS   Zajac,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2023) Microbiology, National Veterinary Research
            Institute (PIWET), Al. Partyzantow 57, Pulawy 24-100, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300x
            Sequencing Technology             :: Complete Genomics
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/25/2023 10:22:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,311
            CDSs (total)                      :: 5,189
            Genes (coding)                    :: 5,055
            CDSs (with protein)               :: 5,055
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 134
            CDSs (without protein)            :: 134
            Pseudo Genes (ambiguous residues) :: 0 of 134
            Pseudo Genes (frameshifted)       :: 38 of 134
            Pseudo Genes (incomplete)         :: 107 of 134
            Pseudo Genes (internal stop)      :: 19 of 134
            Pseudo Genes (multiple problems)  :: 29 of 134
            Pseudo Genes (short protein)      :: 1 of 134
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP117036.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6659
                     /organism="Salmonella enterica"
                     /mol_type="genomic DNA"
                     /strain="PIW95_S14_0299"
                     /isolation_source="feces"
                     /host="pig"
                     /db_xref="taxon:28901"
                     /plasmid="ColRNAI"
                     /geo_loc_name="Poland"
                     /lat_lon="51.42 N 21.98 E"
                     /collection_date="2014"
                     /collected_by="National Veterinary Research Institute"
     gene            join(5597..6659,1..506)
                     /gene="cea"
                     /locus_tag="PQP07_RS26495"
                     /old_locus_tag="PQP07_26505"
     CDS             join(5597..6659,1..506)
                     /gene="cea"
                     /locus_tag="PQP07_RS26495"
                     /old_locus_tag="PQP07_26505"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(503..730)
                     /locus_tag="PQP07_RS26500"
                     /old_locus_tag="PQP07_26510"
     CDS             complement(503..730)
                     /locus_tag="PQP07_RS26500"
                     /old_locus_tag="PQP07_26510"
                     /inference="COORDINATES: protein motif:HMM:NF015489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_229471606.1"
                     /translation="MLYAIVISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            892..1029
                     /locus_tag="PQP07_RS26505"
                     /old_locus_tag="PQP07_26515"
     CDS             892..1029
                     /locus_tag="PQP07_RS26505"
                     /old_locus_tag="PQP07_26515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(2046..2237)
                     /locus_tag="PQP07_RS26510"
                     /old_locus_tag="PQP07_26520"
     CDS             complement(2046..2237)
                     /locus_tag="PQP07_RS26510"
                     /old_locus_tag="PQP07_26520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2302..2625
                     /locus_tag="PQP07_RS26515"
                     /old_locus_tag="PQP07_26525"
     CDS             2302..2625
                     /locus_tag="PQP07_RS26515"
                     /old_locus_tag="PQP07_26525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2834..>3265
                     /locus_tag="PQP07_RS26520"
                     /old_locus_tag="PQP07_26530"
                     /pseudo=""
     CDS             2834..>3265
                     /locus_tag="PQP07_RS26520"
                     /old_locus_tag="PQP07_26530"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3303..3821
                     /locus_tag="PQP07_RS26525"
                     /old_locus_tag="PQP07_26535"
     CDS             3303..3821
                     /locus_tag="PQP07_RS26525"
                     /old_locus_tag="PQP07_26535"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            3828..4082
                     /locus_tag="PQP07_RS26530"
                     /old_locus_tag="PQP07_26540"
     CDS             3828..4082
                     /locus_tag="PQP07_RS26530"
                     /old_locus_tag="PQP07_26540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015059032.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4400..4819)
                     /locus_tag="PQP07_RS26535"
                     /old_locus_tag="PQP07_26545"
     CDS             complement(4400..4819)
                     /locus_tag="PQP07_RS26535"
                     /old_locus_tag="PQP07_26545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(4900..5337)
                     /locus_tag="PQP07_RS26540"
                     /old_locus_tag="PQP07_26550"
     CDS             complement(4900..5337)
                     /locus_tag="PQP07_RS26540"
                     /old_locus_tag="PQP07_26550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     Typing          1884..1968
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.837"
                     /coverage="100.0"
                     /evalue="1.41e-35"
     Typing          complement(1315..1870)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.52"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2616..4186
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.809"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttatcaaac gctgactgaa aaatatggtg aaaaatattc gaaaatggca caggaacttg
       61 ctgataagtc taaaggtaag aaaatcggca atgtgaatga agctctcgct gcttttgaaa
      121 aatacaagga tgttttaaat aagaaattca gcaaagccga tcgtgatgct atttttaatg
      181 cgttggcatc ggtgaagtat gatgactggg ctaaacattt agatcagttt gccaagtact
      241 tgaagattac ggggcatgtt tcttttggat atgatgtggt atctgatatc ctaaaaatta
      301 aggatacagg tgactggaag ccactatttc ttacattaga gaagaaagct gcagatgcag
      361 gggtgagtta tgttgttgct ttacttttta gcttgcttgc tggaactaca ttaggtattt
      421 ggggtattgc tattgttaca ggaattctat gctcctatat tgataagaat aaacttaata
      481 ctataaatga ggtgttaggg atttaattat tctttattga tataaaaagt cctagcaatc
      541 caaatgggat tgctaggacc aaacaaagta gattatatag cataaatagg tttaattttg
      601 ctacgggggc gttatttagg ttttttcttc tttcgaaaaa atctttcttt atgaagttaa
      661 aagctatgta ttcaatagca tattttgaat atggacatag aatagtgctt atcactattg
      721 catatagcat cttatctgac acaaggaaat aatacccttc gctgtttttt gttataaggt
      781 atatatatat aagtgtgcag tacaggccaa ataaaatatt ttttatgtag tatcttaagc
      841 tcataaatta aacctcgcca tatattcttt tcattttata aggatcgagt tatgaggaaa
      901 agattttttg tgggaatatt cgcgataaac ctccttgttg gatgtcaggc taactatata
      961 cgtgatgttc agggagggac catcgcacca tcctcctctt ctaaactgac ggggatcgcg
     1021 gttcagtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1081 gcttgcaaac aaaaaaacca ccgctaccaa cggtggtttg tttgccggat caagagctac
     1141 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     1201 tagtgtagcc gtagtcgggc cactacttca agaactctgt agcaccgttt gtgccatcat
     1261 cgctctgcta atccggttac cagtggctgc tgccagtggc gttaaggcgt gccttaccgg
     1321 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1381 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc aacagcgtga
     1441 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1501 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     1561 tagtcctgtc gggtttcgcc acctctgact tgagcgtcta tttttgtgat gctcgtcagg
     1621 ggggcggagc ctatggaaaa acgcctgcta cgtggccttc ttcctgttct tggtcttttg
     1681 ctcacatgtt ctttccggcc ttatcccctg attctgtgga taactgtgtt accgtttttg
     1741 tgtgagtcag taccgctcgc cgcagtcgaa cgaccgagcg tagcgagtca gtgagcgagg
     1801 aagcggaaaa gcgcctggac gtgcattttc tccttacgca tctgtgcggc atttcacacc
     1861 cggcatggcg tacttttcat acaatccgca ctgatgccgc atggttaagc cagtatacac
     1921 tccgctatcg ctacgtgact ggttcagggc ttcgccccga aaccccctga cgcgccctga
     1981 cgggcttgtc tgctcccggt atccgctcac aggcaagctg tgaccgtctc cgggagctgt
     2041 atgtgtcaga ggttttcacc gtcatccccg aagcgcgcga ggcagctgcg gtaaagctca
     2101 tcggcgtggt cgtgaagcga ttcacagata tctgcctgtt cgtccgcgtc cagttcgttg
     2161 agcttctcca gcagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
     2221 ttttcctgtt tagtcactga tgcctccgtg taagggggta tttctgttca tggggtaatg
     2281 ataccgatga aacgcgagag gatgctcacg atacgggtta ctgatgatga acatgccagg
     2341 ttacttgaac gctgtgaggg taaacaactg gcagtatgga tgcggcgggt ctgcctgggt
     2401 gagccggttg cccgttccgg gaagctgccg acactggcac cgccgttact gcgtcagctg
     2461 gccgccatcg ggaataacct gaatcagaca gcgaggaagg tgaacagcgg gcagtggtct
     2521 tccggtgacc gggttcaggt ggtggccgca ctgatggcca tcggggatga gctgcgccgg
     2581 ctgcgtctgg ccgtcaggga acagggggcg cgggatgata gttaaatttc atgccagggg
     2641 aaaaggtggt ggcagtggtc cggttgatta cctgctgggg agggagcgta accgcgaagg
     2701 cgcaacggtg cttcagggta atccggaaga agtccgggaa ctcatcgatg ccacgccatt
     2761 tgcgaagaaa tacacgtccg gtgttctgtc gttcgcggag aaggagctgc cgccgggagg
     2821 acgtgaaaaa gtgatggcga gctttgagcg tgtactgatg cccggtctcg aaaaaaatca
     2881 gtacagcatc ctgtgggtgg agcaccagga caagggacgg cttgagctga attttgtcat
     2941 tccgaacatg gagctacaga ccggaaaacg cctccagccg tactacgacc gcgcagacag
     3001 gcctagaatt gatgcctggc agacgctggt gaatcaccat tacgggctgc atgacccgaa
     3061 cgccccggag aaccgcagga cgctgacact ccctgataac ctgcctgaaa cgaaacaggc
     3121 gcttgctgag ggcgtcacgc gaggtataga tgcactttac catgccggag agataaaagg
     3181 ccgtcaggat gtgattcagg cgctcactga ggcggggctg gaagtggtca gggtgacgcg
     3241 aagcagtatc agcattgcag atccgaacgg cgggaagaat atcaggctga aaggagcatt
     3301 ttatgagcaa tcttttgcag acgggcgcgg agttcgagaa aaagctgaaa gagagagccg
     3361 aatctacaga gaaaatgctg aacaacgagt tcaggaggct cggcgaatct gtaagcgagg
     3421 ctgtgacatc aaacgagacg aaaatcagag acgctatagc cctgttcaca gcctcgacag
     3481 aggaatcgct ggaaaaacac cgggaagggg tgaaagaggc gatgatgcag cacaggaggg
     3541 acgtgttaaa gctggcaggg aatacgggca tgatgttact ggggatagtc tttctcctgt
     3601 ttaccgcgag tggcgggacg ctctggtatc ttggagggag gatacaggcg aacctggaag
     3661 aaatcaggaa gcaggaagag acattgcaga aactgaacgc gaagacatgg ggcgtggagt
     3721 ttgtgcagga cgggaacagg aaattccttg tccttccgta cgggaaatca gcggaggtga
     3781 ttccctttca ggggaaagag tgggtacatc tgaaggagtg acacagagtg acagagctgg
     3841 aaacacattt gctgaacgcc ttagagcagc tgcaacagga ctatatgcag cggctgagcg
     3901 aatgggagag cgccttcgtg gaattgcaga agatgttttc gcttacgcaa cgggacaacg
     3961 cgatgctgaa cgagcgggtc atgcagttga gtcagcaggt gcagcacttg agcgagcaga
     4021 cagaacgctt gagcgagcag acagaacgct tgagccagtt atacagcgag aactggagat
     4081 aagagaggaa cggctgatac aggagcgcga acatgtgtta tccctggaac gggagcgtca
     4141 gccggaaata caggaacgca cgctggatgg cccttcgctg ggatggtgaa accatgaaaa
     4201 atggcagctt cagtggatta agtgggggta atgtggcctg taccctctgg ttgcataggt
     4261 attcatacgg ttaaaattta tcaggcgcga tcgcggcagt ttttcgggtg gtttgttgcc
     4321 atttttacct gtctgctgcc gtgatcgcgc tgaacgcgtt ttagcggtgc gtacaattaa
     4381 gggattatgg taaatccact tactgtctgc cctcgtagcc atcgagataa accgcagaaa
     4441 tcgtgtcagc cagcagcctg gattttttcg gggtaaggtc tttgctgcgg taatcagtac
     4501 ccgcaaattg agttgtgccc cggagtgagt ttaaaaattc ctggctgtga aactgctgaa
     4561 tgcgttgttg cacatcactc cgtgattttc ctgtcgcttt atctttagct cccatatccc
     4621 agaactgccg aaaaaaaggc accatcattc gtgttgtgtc ggctttttgg gtggaaaagt
     4681 tgggatcaaa accgtcatca gaagcatata cgtaatgccg ggtatgttgt tccacgctgc
     4741 cctttggggt acaggctgtg agagctactg ttgctgttat catggcgata agacaaagtt
     4801 tgtttccggt acgtctcatg agtgtctccc taccttaaag tatttattct cggtgagtta
     4861 ttatccgaag actattttat catcagtctc tgacagcgtt cagcgaccga acaacctgga
     4921 ccagaatccc cgtttctttt ccagttccag tgcctgcctg agctgggcta tctcattttg
     4981 tagctgttcc cgttctgctt cctggcgtct gcgatccata tcctgtgcct gtttatcctc
     5041 aagcatcagc gtcaggcatt gtttcagctc attcagctcc cggagaatgc gttctgtctg
     5101 ctgatcatgt ggattttctg catgtccctc actgtgcctt tctggtgtct cattctgctt
     5161 taattcgccg tatgcccgga tcagttcact ggtttcaaac tcccgtcgtc cgtccctgcc
     5221 ggtacggtaa ctgacaaggc ctgcgcgcat atcacggtaa aactgtgaac gcgatctgcc
     5281 tgtcattttt agtgcgtccc ggagtgtgtg ccatgccata aagtgacagt gtcccataga
     5341 tgtctcatct catagtttca gtaaaacata atgaggtctg agaacggtaa tgtttgtgct
     5401 ggtttttgtg gcatcgggcg agaatagcgc gtggtgtgaa agactgtttt tttgatcgtt
     5461 ttcacaaaaa tggaagtcca cagtcttgac agggaaaatg cagcggcgta gcttttatgc
     5521 tgtatataaa accagtggtt atatgtacag tatttatttt taacttattg ttttaaaagt
     5581 caaagaggat tttataatgg aaaccgcggt agcgtactat aaagatggtg ttccttatga
     5641 tgataaggga caggtaatta ttactctttt gaatggtact cctgacggga gtggctctgg
     5701 cggcggaggt ggaaaaggag gcagtaaaag tgaaagttct gcagctattc atgcaactgc
     5761 taaatggtct actgctcaat taaagaaaac acaggcagag caggctgccc gggcaaaagc
     5821 tgcagcggaa gcacaggcga aagcaaaggc aaacagggat gcgctgactc agcgcctgaa
     5881 ggatatcgtg aatgaggctc ttcgtcacaa tgcctcacgt acgccttcag caacagagct
     5941 tgctcatgct aataatgcag ctatgcaggc ggaagcagag cgtttgcgcc ttgcgaaagc
     6001 agaagaaaaa gcccgtaaag aagcggaagc agcagaaaag gcttttcagg aagcagaaca
     6061 acgacgtaaa gagattgaac gggagaaggc tgaaacagaa cgccagttga aactggctga
     6121 agctgaagag aaacgactgg ctgcattgag tgaagaagct aaagctgttg agatcgccca
     6181 aaaaaaactt tctgctgcac aatctgaagt ggtgaaaatg gatggagaga ttaagactct
     6241 caattctcgt ttaagctcca gtatccatgc ccgtgatgca gaaatgaaaa cgctcgctgg
     6301 aaaacgaaat gaactggctc aggcatccgc taaatataaa gaactggatg agctggtcaa
     6361 aaaactatca ccaagagcca atgatccgct tcagaaccgt cctttttttg aagcaaccag
     6421 acgacgggtt ggggccggta agattagaga agaaaaacaa aaacaggtaa cagcatcaga
     6481 aacacgtatt aaccggataa atgctgatat aactcagatc cagaaggcta tttctcaggt
     6541 cagtaataat cgtaatgccg gtatcgctcg tgttcatgaa gctgaagaaa atttgaaaaa
     6601 agcacagaat aatctcctta attcacagat taaggatgct gttgatgcaa cagttagct
//
