LOCUS       NZ_CP116997            12602 bp    DNA     linear   CON 07-JUN-2024
DEFINITION  Escherichia coli strain MLI108K2 plasmid p12_MLI108-2, complete
            sequence.
ACCESSION   NZ_CP116997
VERSION     NZ_CP116997.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29553892
            Assembly: GCF_024331375.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12602)
  AUTHORS   Riediger,M. and Zautner,A.E.
  TITLE     Molecular epidemiology of Escherichia coli with resistance against
            third-generation cephalosporins isolated from stool samples of
            European soldiers with diarrhea on deployment in Western African
            Mali
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12602)
  AUTHORS   Riediger,M. and Zautner,A.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2023) Institute of Medical Microbiology and
            Hospital Hygiene, Medical Faculty, Otto-von-Guericke University
            Magdeburg, Leipziger Strasze 44, Magdeburg, Saxony-Anhalt 39120,
            Germany
REFERENCE   3  (bases 1 to 12602)
  AUTHORS   Zautner,A.E. and Riediger,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUL-2022) Institute of Medical Microbiology and
            Hospital Hygiene, Medical Faculty, Otto-von-Guericke University
            Magdeburg, Leipziger Strasze 44, Magdeburg, Saxony-Anhalt 39120,
            Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-JAN-2023
            Assembly Method :: trycycler v. 0.5.3; unicycler v. 0.5.0; skesa v.
            2.4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_024331375.2-RS_2024_06_07
            Annotation Date                   :: 06/07/2024 03:09:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,991
            CDSs (total)                      :: 4,866
            Genes (coding)                    :: 4,605
            CDSs (with protein)               :: 4,605
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 84 of 261
            Pseudo Genes (incomplete)         :: 184 of 261
            Pseudo Genes (internal stop)      :: 54 of 261
            Pseudo Genes (multiple problems)  :: 54 of 261
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116997.1.
            Bacteria and source DNA available from Institute of Medical
            Microbiology and Hospital Hygiene Medical Faculty Otto-von-Guericke
            University Magdeburg 39120 Magdeburg, Germany.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MLI108K2"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p12_MLI108-2"
                     /geo_loc_name="Mali: Koulikoro"
                     /lat_lon="12.52 N 7.34 W"
                     /collection_date="2014-07-24"
                     /collected_by="Bundeswehr Medical Service"
     gene            <1..388
                     /locus_tag="NL410_RS01620"
                     /old_locus_tag="NL410_001615"
     CDS             <1..388
                     /locus_tag="NL410_RS01620"
                     /old_locus_tag="NL410_001615"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_226138557.1"
                     /translation="RHYGRYMANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVL
                     NERYGLNISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPDNGFFLSGNDF
                     MRAEEESQRVVESFRDSLSRKKGF"
                     /name="hypothetical protein"
     gene            473..1456
                     /locus_tag="NL410_RS01625"
                     /old_locus_tag="NL410_001620"
     CDS             473..1456
                     /locus_tag="NL410_RS01625"
                     /old_locus_tag="NL410_001620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_151166972.1"
                     /translation="MKKKFSIIVPVYNVEKYLRECIQSVLDQDFNDYEIICVNDGSTDG
                     SLDIILSFDDQRIKVINQENKGLSVARNVAIESSTGEYIWFLDSDDKISHGSLKLIDKI
                     TNEKNYGIIFFNTHVFFDTPELKKKGWHDYKRSTFGEMDALTFFEKEMTNKKLIVSACC
                     YVFRRELLNGNAFIPHIYHEDNHFIATLIFDNYSTICLSLDEALYLRRVRNDSIMTSKK
                     SMRHIIGYSTCIDELTKYKKTKGIQNKSFDRFVNGMVLSLFSIAETIKTKEAMNYVARY
                     KKHPDLSFKDRALINYPLIKKTYLKLKNGKVFNYLRNGIINKKSNP"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(1915..2612)
                     /locus_tag="NL410_RS01630"
                     /old_locus_tag="NL410_001625"
     CDS             complement(join(1915..2363,2363..2612))
                     /locus_tag="NL410_RS01630"
                     /old_locus_tag="NL410_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_223368647.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEHWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            2771..4315
                     /locus_tag="NL410_RS01635"
                     /old_locus_tag="NL410_001630"
     CDS             2771..4315
                     /locus_tag="NL410_RS01635"
                     /old_locus_tag="NL410_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="WP_201360865.1"
                     /translation="MKNYDVFYGGIFKTITDYKLLKIQKDIKNNMTALVSSFKWVGIGQ
                     LVKIATQLVSLFYLTHLIPPSSYGLLAMSSVVMNLASIFNDMGTGAAVIQRQEASIGFY
                     NYIYRINIFTGLFVMLLVIITSPLIVVYFNTPELYPVLFLLAFSFPISSLTITHKAKLE
                     KEMNFKVIIAIEIFSSLTGMVLAIVLANLGFGVYSLIFQTLTTISISTICFLKVSGLSL
                     SLRIKNISHSDKSGVIGFSGYLLSFNLINYFSRNLDSILIGRFFSSAILGVYSIAYRIM
                     LFPLQSLTLVFSRVFLPHLSKNINNTNNTNKQDYLNALKLILSLSAPLMLGLSATSFSF
                     TRIFFDPKWHLLGDLLHWLAPTAIIQSALSTTGSVFMAYGKTRWLFFLGCIGAFLMGLS
                     FIIGVHFDIKTLVYLYFFANLINSLPAFILLKNILKFSYIELVNVFFRTISPAFLMFLL
                     IKVWQHFSFFKNASIDFSFNVALGIFSYIVFYTLFNFNEVVRTGVIHKLRCMTKLK"
                     /name="lipopolysaccharide biosynthesis protein"
     gene            complement(<4408..4672)
                     /locus_tag="NL410_RS01640"
                     /old_locus_tag="NL410_001635"
                     /pseudo=""
     CDS             complement(<4408..4672)
                     /locus_tag="NL410_RS01640"
                     /old_locus_tag="NL410_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /name="IS1-like element transposase"
     gene            complement(4817..5693)
                     /locus_tag="NL410_RS01645"
                     /old_locus_tag="NL410_001640"
                     /pseudo=""
     CDS             complement(4817..5693)
                     /locus_tag="NL410_RS01645"
                     /old_locus_tag="NL410_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946055.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(5892..6176)
                     /gene="nadS"
                     /locus_tag="NL410_RS01650"
                     /old_locus_tag="NL410_001645"
     CDS             complement(5892..6176)
                     /gene="nadS"
                     /locus_tag="NL410_RS01650"
                     /old_locus_tag="NL410_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_032242301.1"
                     /translation="MSFFDELKASLEEAVEIKNGTKAPARVTRYELADVRAIRENLNIT
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFDELAAH"
                     /name="nadS"
     gene            complement(6178..6510)
                     /locus_tag="NL410_RS01655"
                     /old_locus_tag="NL410_001650"
     CDS             complement(6178..6510)
                     /locus_tag="NL410_RS01655"
                     /old_locus_tag="NL410_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_032242302.1"
                     /translation="MSHAIEFIETTMFTRQIKQIATDDEIKELQKELIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSITDAEKAELKKLTQ
                     RLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6655..6891)
                     /locus_tag="NL410_RS01660"
                     /old_locus_tag="NL410_001655"
     CDS             complement(6655..6891)
                     /locus_tag="NL410_RS01660"
                     /old_locus_tag="NL410_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_032242304.1"
                     /translation="MTEMERQLLSALESLQTSYEQQKQAWQDSYSSLQRMFETTSQALE
                     RNDRVCLNLSNQVNALHEQVERLSSDLSRLTRS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            7236..7604
                     /locus_tag="NL410_RS01665"
                     /old_locus_tag="NL410_001660"
     CDS             7236..7604
                     /locus_tag="NL410_RS01665"
                     /old_locus_tag="NL410_001660"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032242306.1"
                     /translation="MFSYLKAMYHQSKIQAELKAQIHEQTTVNAICHHPESIEIIAVCS
                     TDAYYRKRKDAAFLTTCSVLMRTLKDESVPMVLRKTAWRLLNERYQRIKLNQAYRIENF
                     LLVADFEYALEEHDELAE"
                     /name="hypothetical protein"
     gene            complement(8034..8168)
                     /locus_tag="NL410_RS01670"
                     /old_locus_tag="NL410_001665"
     CDS             complement(8034..8168)
                     /locus_tag="NL410_RS01670"
                     /old_locus_tag="NL410_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072003336.1"
                     /translation="MHEPPVQSDRCVLSGNYRLESNPERHAKTPLAAAIGKQDVREMQ"
                     /name="replication initiation protein"
     gene            complement(9352..9669)
                     /locus_tag="NL410_RS01675"
                     /old_locus_tag="NL410_001670"
     CDS             complement(9352..9669)
                     /locus_tag="NL410_RS01675"
                     /old_locus_tag="NL410_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="WP_032242326.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAVNVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEETGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAD
                     Q"
                     /name="lipoprotein"
     gene            10754..10903
                     /locus_tag="NL410_RS01680"
                     /old_locus_tag="NL410_001675"
     CDS             10754..10903
                     /locus_tag="NL410_RS01680"
                     /old_locus_tag="NL410_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_167776965.1"
                     /translation="MPSEHDEQLDSTDTQLPGEYDLFDLMAQVTEENLHALVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            11363..12591
                     /locus_tag="NL410_RS01685"
                     /old_locus_tag="NL410_001680"
     CDS             join(11363..11678,11678..12591)
                     /locus_tag="NL410_RS01685"
                     /old_locus_tag="NL410_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="WP_088895425.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQ
                     LFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVEYG
                     RGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLR
                     IHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVPPSKR
                     AHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQ
                     DVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGI
                     AESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACN
                     GLSDNRCLEI"
                     /name="IS3-like element IS2 family transposase"
     Typing          10546..10719
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.143"
                     /coverage="100.0"
                     /evalue="5.58e-78"
     Typing          complement(8061..8426)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.185"
                     /coverage="89.0"
                     /evalue="2.91e-169"
     Typing          complement(10071..10579)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.51"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aagacattat gggcggtata tggcgaattt taaccctggt ccaagaacag ccattgcagc
       61 tgtcagggat tccaagaacg atatagaaac ctggaagaaa aatggtattc gtagggaaga
      121 gattgtgact gtgctgaatg aacgttacgg cctgaatatc tcactggttg cgtttgataa
      181 ggctctttac agattgagaa aaagtacaga tgaaccgtct cttgctgatg tggattctgt
      241 gtctgctgca gaaacgaatt tctctgaagc cagtgggcct gacaatggtt tttttctgtc
      301 cgggaatgac ttcatgcgag cagaagaaga atctcaaaga gtggttgagt cattcagaga
      361 tagccttagc aggaagaaag gattctgatt tattcaacaa agccgttgat aaatcgcatt
      421 ccataaataa caacttgatg ttagtataac taaatattgt ttggagatta ctgtgaaaaa
      481 aaaattttcg ataatagtac ctgtatataa cgttgaaaaa tacttaaggg aatgtattca
      541 atcagtatta gatcaggatt ttaacgacta tgagataatc tgtgtaaatg atggttcaac
      601 agatggcagt ctagatatca tactgtcatt tgatgaccaa agaatcaaag taataaatca
      661 ggaaaataaa ggcctgtcgg tggcaagaaa tgtcgccatt gaatcatcaa ctggtgaata
      721 tatatggttt ttagatagtg atgataaaat atcacatgga agtctaaagt taatagacaa
      781 gataaccaat gaaaaaaatt atgggattat tttctttaat acacatgttt tttttgacac
      841 tccagaactg aaaaaaaaag gctggcatga ttataagcgt tcaacatttg gtgaaatgga
      901 tgcattaact ttctttgaaa aagaaatgac gaacaaaaaa ctaatagtgt cagcatgctg
      961 ctatgttttt agaagggaat tattaaatgg caatgcattc attccgcata tatatcatga
     1021 ggataatcac tttatagcaa ccctgatttt tgacaattat tcaactatat gcctctcgtt
     1081 ggatgaggcg ttatatctta ggagggtaag aaatgattcc ataatgacgt ctaaaaaatc
     1141 aatgaggcac atcattggat attcaacatg catcgatgag cttacgaagt ataaaaaaac
     1201 aaaaggcata caaaacaaga gctttgatag atttgtgaat ggaatggttt tgtcgttatt
     1261 ctctatagcg gaaacaataa aaacaaagga agcaatgaac tatgtggcga gatataaaaa
     1321 acaccccgac ctatcattta aagatagagc attaattaat tacccattaa ttaaaaagac
     1381 ttatttaaaa ctaaaaaacg ggaaggtgtt taattaccta aggaatggca ttattaataa
     1441 aaaaagcaac ccttaataaa agcaaagcga taattaataa aggtttttct ttgttgaata
     1501 aataagattt cggataggta tccccgtgac gggttgggtt ttcaggcgat acgcacgctt
     1561 tccggggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga tcggggtcag
     1621 tttggagatc ggaaattttt gtacgttaag cgcattattt aaccaatctt ggttcgtaag
     1681 gggttgattt tgcgtcagtg gctatcgctt ttaagttgtc gctcttgcag tatcgcattt
     1741 aggttatccg attaaggtca aacctctgaa aatgccgtat agcgcgggag tacaccctat
     1801 tgctataggt aagtctgttc aaaaaacagg cttaccgtac aataattctc tatatccaaa
     1861 ctaaccccag atcacgcatc ttcccgacaa cgcagaccgg gtaatgactc caacttactg
     1921 atagtgtttt atgttcagat aatgcccgat gaccttgtca tgcagctcca ccgattttga
     1981 gaacgacagt gacttccgtc ccagccttgc cagatgttgt ctcagattca gattatgtcg
     2041 ctcaatgcgc tgcgtatatc gcttgctgat aacgtgcagc tttcccttca ggcgtgattc
     2101 atacagcggc cagccatccg tcatccatac cacgacctca aaggccgaca gcaggctcag
     2161 aagacgctcc agtgtggcca gagtgcgttc accgaagacg tgcgccacaa ccgtcctccg
     2221 tatcctgtca tacgcgtaaa acagccagcg ctgacgtgat ttagcaccga cgtagcccca
     2281 atgttcgtcc atttcagcgc agacaatcac atcacttccc ggttgtatgc gcgaggttac
     2341 cgactgcggc ctgagttttt taagtgacgt aaaaccgtgt tgaggccaac gcccataatg
     2401 cgtgcactgg cgcgacatcc gacgccattc atggccatat caatgatttt ctggtgcgta
     2461 ccgggctgag aggcggtgta agtgaactgt agttgccatg ttttacggca atgagagcag
     2521 agatagcgct gatgtccggc agtgcttttg ccgttacgca ccacgccttc agtagcggag
     2581 caggaaggac atctgatgga aatggaagcc acgcaagcac cttaaaatca ccatcataca
     2641 ctaaatcagt aagttggcag cattacccac tattggtgga gatgatcata atgccttata
     2701 atcatacggg atacgaactt ataaagtcct aaagtttagc taaatcggaa tctacaacgt
     2761 agatgatagc ttgaagaatt atgatgtctt ttatggtggt atctttaaaa ccatcacgga
     2821 ttataaatta ttgaaaattc aaaaggatat taaaaacaat atgactgcac tagtcagtag
     2881 ttttaaatgg gttggcattg gacaacttgt aaaaatagca acacaattgg tttcgttatt
     2941 ctacttgacg cacttaatcc ccccatcgag ctatggacta ttagcaatgt catctgtggt
     3001 tatgaattta gcaagcatat tcaatgacat gggaactggg gcggcagtaa ttcagcgaca
     3061 agaggcgtcg atcggttttt ataattacat atacaggata aacattttta ctgggttgtt
     3121 tgttatgctg cttgtaataa taacatcccc attaattgtc gtgtacttta acacacccga
     3181 gctatacccg gttctatttc tattggcttt tagctttcca atttccagct taacaataac
     3241 tcacaaagca aaattagaaa aagaaatgaa ctttaaagtc atcattgcaa ttgaaatttt
     3301 ctctagcctt acaggaatgg tgctggcaat agttttagct aatttaggct ttggtgttta
     3361 tagtttgatt tttcaaacac tgacaacaat atcaatatcc acaatatgtt ttttgaaggt
     3421 ttccgggttg agtttatcat tgagaattaa aaatatcagc catagtgaca agtcaggagt
     3481 tatagggttt agtggttatt tattgtcttt taatttaata aattattttt ccaggaatct
     3541 tgattctatt ttaataggcc gttttttctc atccgcaata cttggggtct attcgattgc
     3601 ctaccgcatt atgttatttc cgttgcagag cctgacgctt gttttttcta gagttttctt
     3661 gccacacctt agcaaaaaca taaataacac taataacact aataaacaag attacttaaa
     3721 tgcattaaaa cttatactat cattaagcgc gccactgatg ctaggtttat ctgctacatc
     3781 tttttctttt acgaggattt tctttgatcc taagtggcac ctacttggag acttattgca
     3841 ttggttggca cctacagcga ttatccaatc agccttgagt acaacaggtt ctgtttttat
     3901 ggcctacgga aaaacaaggt ggttgttttt cttaggttgc attggcgctt ttttgatggg
     3961 gttgtctttc attataggtg ttcatttcga tataaaaaca ttggtctacc tttatttttt
     4021 tgcaaactta attaattcac tcccggcatt tattttactt aagaatatct tgaagtttag
     4081 ttacattgaa ttggtaaacg tgttttttag aactatctcc ccagcttttt tgatgttctt
     4141 acttattaaa gtatggcagc atttttcctt tttcaaaaat gccagcattg atttttcttt
     4201 taatgtcgca ttgggtattt ttagttatat tgttttttat accttgttta atttcaatga
     4261 ggttgttagg acaggagtaa ttcataagtt aagatgcatg accaagttaa agtgattcat
     4321 ttattaaatt cattaaatga attggtaatg actccaactt actgcttgtg ttttatgttc
     4381 agataatgcc cgataccgac ttcggcctaa tttttttaag tgtcgtaaaa tcgtgttgag
     4441 gacagtgccc ataatgcggg atgtggcccg gcatccaacg ccattcatgg ccatatcaat
     4501 gattttctgg tgtgtaccgg gctcagaggc ggtgtaagtg aactgtagtt gcttgctatg
     4561 ttttacgaca gtgagagcag agatagcgtt gatgtccggt agtgctttta ccgttacgca
     4621 ccacgccttc agtagctgaa caggagggat aactgatgga aatggaagcc acgggagcac
     4681 ctcaaaaaca ccattagaca ctaaaccagc aagttggcat catcacaaac agttgttact
     4741 gctggaaaaa aaacactatc aacacttaag taatggtgat tcaataatta agattcagta
     4801 tgtaaaatac tgaatcttaa ttataaattt tcctttttct aaaaaatgta gcgcgactaa
     4861 tacctaaagc aacccaaggc tcccctagag acagcctacc tcctcctgat gatttttcac
     4921 caccaacttt tcctcttttg gcttgtataa gtgaaaaatt ttgcttatta aatttcaaaa
     4981 atgtccactt gcagatactt ttcgctattc ccgttacttc attctcattc aaaggcactg
     5041 caaattgcag attgtaagcg caggcgcgtt catggcaggc ttgtagccac tgttcgtact
     5101 caggccagcc ctgacggata gcgcgatatg cccactttct ggttttatca aacagtatgc
     5161 agttacgacc taagccataa tcggcgacga tttttttatc gttcgcagcg ttcaggtcga
     5221 gtgattcggc taaccagtcc agcgtgtaga gttcaggctg ccatacgggc aattttccag
     5281 tgactgtggt tcggattctt gcaaattagc cccgaatacc ccgtgtcagc gcccaatttt
     5341 ttacgtagcg cgctctctac agcagcggcg taacgtaacg gtttcatttt tccgtctggc
     5401 gcggtgcgta tcgaggtttt cagtgcgtac agcaggtgcg cgtgtccgtt ttcggggttg
     5461 gtgatggtca gtgtcggcgc gggggcgttg cggtcgctcc agtcaatggc tgcgccgatg
     5521 cggtcaacgt caaatcccag ccagaacatg gcgtgaggct gattaaactg gatgtattta
     5581 gccagaatag cgcgctcttt ccctgcgatg cggacaccaa agtgtaaatc atccgagaag
     5641 tacggtttat gcggcaagcg gtcgttgaaa agttcgagtg ctgcgttacg caatgtgccc
     5701 tgaacttatg gcgggctgtt gtctggcacg gttcatgtgc taatatcctt cttaggtttt
     5761 atacctagcc ctgatttatc atgtctcttc cgccaagttg aaatcatgat tatcggggct
     5821 ttgttttttc tgttgccgtt aaagttacac gcgaacggcc tcgcctgtcg aatttcttct
     5881 gtcacatcag cttaatgcgc cgccagttca tcaaaaaact ttgggttagc ctgaatggtg
     5941 gccagcactt ttgctgccag tccggttggg ttacggcgtc cggtttccca gcttttgatg
     6001 gtatcgaggc tggttcccag cactttcgcc atttcgctct gagtaatatt caggttttct
     6061 ctgatagccc tgacgtctgc cagctcgtag cgggtcacac gggcgggggc tttggttccg
     6121 tttttgattt caacggcctc ttccagagag gctttcagct cgtcaaaaaa actcatctta
     6181 cacctcgtct ttcagtcgtt gggtcagctt cttcagttct gctttctctg cgtctgtgat
     6241 gctgtctttg gtgcttttag ggtaggccat caccagataa atcacctctg cggtggcaag
     6301 gaagtaaatc acccgcgcac tgccgctctt tccctgattc cccgtcgcca tcctgatctt
     6361 tcgcagaccg cccgttttct ggatgaggtc acctttgtcc ggtgactcga taagctcttt
     6421 ctgaagctct ttgatctcat catccgtggc gatttgcttt atctgacggg taaacatcgt
     6481 tgtctcgata aactcaatcg cgtgactcac ggctgtctcc tgtatatttt gatgtacaat
     6541 gtacaccaag aggcgtgttc tgaaaagccg cgttctgtgc catcagacgt tacatggact
     6601 gacctcgata gcgggttgct tgcttctcct gctgtaactg ccgttcctgc tgctttacga
     6661 gcgtgttaat cggctcagat cgctgctcaa gcgttcgacc tgttcgtgca aggcgttcac
     6721 ctgattgctc aagttcagac ataccctgtc gttgcgctcc agtgcctgtg aggtagtctc
     6781 gaacatgcgt tgcaagctgc tgtaactgtc ctgccacgcc tgcttctgct gttcgtagct
     6841 tgtttgtaag ctttctaagg cgctcagaag ctgcctttcc atctcggtca tggtttttcc
     6901 tgtcctcagt gataaaaacg tgctgtggcg tttctgtggt gtctgacggg gttatttcgc
     6961 ggtgtacttc atcacggtgt ttccggctgt cgatgtgtat gtctcggtgc catgcgcttt
     7021 cttcggcagt accaccagat aggtgttctt ctgcttgtcg tccttgtaaa tcgctacccc
     7081 tgcgccgctc tggggtccgc ttggaaaaag gaaattatcc tacgctaagc gcattttttg
     7141 cacagatggc taccgactga acgtaacgtg tgagtcatga tcagaaatgt gttaactcta
     7201 ggaattgaca cgacttactg tttgatgggg aaatcatgtt tagctatctc aaggctatgt
     7261 atcatcaatc aaaaattcag gctgaactca aggcacagat acatgaacaa actacggtta
     7321 atgctatctg tcatcaccct gaatcaatcg agatcattgc ggtttgttcg actgatgcgt
     7381 attaccgaaa acgtaaggac gctgcttttc tgacaacctg ttctgtgctc atgcgtactt
     7441 taaaagatga atccgtaccg atggtactac ggaaaacagc atggcgatta ttgaacgaaa
     7501 gataccaacg aattaagtta aatcaggctt ataggataga aaatttcttg cttgttgccg
     7561 attttgaata tgctctcgaa gaacatgatg aactagcaga gtagtactag tgaggcgatg
     7621 aataccgaca gttaacgtgc atgtgactta aacgtccgat tacgtacagc atgtagacaa
     7681 catgtccgaa agtaagcaaa accattaacg gacagccgga tcatttcgta catcccttac
     7741 ggtcaggggt tcgcgctcaa tggaacgaaa tcacccgtta gcttaaaacg cttaacgtag
     7801 gataatttcc tttttccaag cggacccgca atacagcaag gaacttcttg agatcctttc
     7861 tgttgcgtcg taatctcttg ctctgtaaac gaaaaaacca ccctggcagg tggtttttcg
     7921 aaggttagct aatcctggca gattttctaa ccgcggtaac agtcttgtgc gagacatgtc
     7981 accaaatctg tcctttcagt gtagccgcac tcaggccatc acttcaagaa ctcttactgc
     8041 atctctcgca catcctgttt accaatggcc gctgccagtg gcgtttttgc gtgtctttcc
     8101 ggattggact caagacgata gttaccggac aagacgcagc ggtcggactg aacggggggt
     8161 tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga
     8221 atgagacaaa cgcggccata acagcggaat gacaccggta aaccgaaagg caggaacagg
     8281 agagcgcacg agggagctac cagggggaaa cgcctgggat ctttatagtc ctgtcgggtt
     8341 tcgccaccac tgatttgagc gtcagattct gtgatgcttg tcaggggggc ggagcctatg
     8401 gaaaaccggc agcgccgcgg cctttcctcc ctcgctttat ctcgtcttca tcgccatatg
     8461 cctttgtgaa tattctccgt ccgctcgccg cagccgaacg accgagcgta gcgagtcagt
     8521 gagcgaggaa gcggaatatc ttcggcctca cgtccggcac cttacactcc gtaactgcat
     8581 tttttcatag gatgcaggat agggctaacg ccgcaaagtg acgtcacctg acgttcctgg
     8641 attacaaagg ttaaagcagc ggacaggaaa tgttttgtgc ccacagctat gctgggttat
     8701 tcacaaaaca gcgggacaaa atgtgttttt ggagtaccgc cggcccgcta aagactcctg
     8761 gttagctccc gcatgtttgc tatctttttg ctaccatttt gctacttttt tgctatctcc
     8821 agagtcttct ggtctttcgg ttgctatcct tttgctactt ttttgctatc aaaatgctac
     8881 ctctcccttc ttgcaataaa tgaccaaggc acgttagcga tgtatacatc gccgcgccgc
     8941 aagggtgccc cttccgaatc ccgttcatca ctgtgtgctg attaatccag gttaaatccg
     9001 acccccttag caataaatcc tgccatgcct gacggcctgg ccaacgcctc acggtcgcac
     9061 agtaaactgc gcaccctttc accgttatcg gtggggttcg tggtggcttt cagggcgctc
     9121 gccggatttt agccgctaaa atgacgtcca tgcgttcgtg tgcatagcca tgcagacgcg
     9181 aagaaatcat caggcgcgtt ttttcacgat atggacaggg aaagatgccg gaaataggca
     9241 ggagggcgca gaaatgccct gagggcgtct gagatggtgc acgcaacgga tgttatggta
     9301 aattcagctg attggcaggt acaaaaaaag ccccgagggc ttttttgtac cttattgatc
     9361 tgcacacttt gcagccttca gggcttccag ctgatttagc cggttagctg cttttttgcg
     9421 ggcttcgcct tttgccatgc catttcctat cccaaaatca cccaggaaac ctaatacggt
     9481 gcgcccgtca aattcgccgg tttcctcaat ttcttgttgt acactgcgcg ttttggcgat
     9541 ttccagagct accgctttgc aatccattgc ggaagattct ggaccggtga cgttcacggc
     9601 ctgtggatat tgctttgttg cgcatcctgc catgatcagc gctgccgata ggatgaatat
     9661 ttttttcaaa attatcctta tttatttagg catagttaag agatacccat tttatcggca
     9721 ggaaatgagg gttttttagt tgaattttgg gaggggcaag gttccatatt ggtcgaggat
     9781 tctggagctg accggcaacg gccagcgcgg ctatgggcca tgcggtggtt gagcatagaa
     9841 aggatcttct tgagttcctt tttttctacg cgtaatctct tgctctgaaa acgaaaaaac
     9901 cacctgggga ggtggttttt cgaaggttaa gtcagttggg gaactgctta acctggtaac
     9961 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
    10021 ccacttcaag aactctatag caccacctac attaactcgc tctgctaatc ctgttaccag
    10081 tggctgctgc cagtggcgct ttgtcgtgtc tttccgggtt ggactcaaga cgatagttac
    10141 cggataaggc gcagcagtcg gactgaacgg ggggttcgtg catacagtcc agcttggagc
    10201 gaactgcctt cccggaactg agtgtcaggc gtggaatgag acaaacgcgg ccataacagc
    10261 ggaatgacac cggtaaaccg aaaggcagga acaggagagc gcacgaggga gccgccaggg
    10321 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc accactgatt tgagcgtcag
    10381 atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa acggctttgc cgcggcccta
    10441 tcgcttccct gttaagtatt tccctggcat cttccaggac atctccgccc cgttcgtaag
    10501 ccatttccgc tcgccgcagt cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg
    10561 aatatatcct gtatcacata ttctgctgac gcaccggtgc cgcctttttt gtcctgccac
    10621 atgaagcact tcactgactc cctcatcagt gccaacatag taagccagta tacactccgc
    10681 tagcgctacg tgactggttc agggctgcgc cccgaaaccc tctaacaacc tctcagctac
    10741 tggatatcac cccatgccat ctgaacatga tgaacaactg gattcgactg atacgcagct
    10801 tcccggcgaa tatgaccttt ttgatttaat ggcgcaggtt actgaagaga acctgcatgc
    10861 cctggtggat tttgggccgc ctgtcggccg ggaagcgtta tagctttttc gcttacccgc
    10921 gtacgtggta atacgccggt attacatctt atttctttat tttcatgtaa ttacaggtgg
    10981 ttgtaatacg ttgcgtatta cataaataaa cggggacttc agtttttatc ttttaattca
    11041 tgcaattgcg atgaaaatcg cggttgcgta tggtgtatga ccatttaagg gataatgtaa
    11101 cctgactttt aaagaatgaa tctatctttg gcagcgcatg agatcgataa aaagcgttcc
    11161 acatcaggcg gttcatgtat ccatgaaccg gaaaggacaa aaataagaca attgtaagac
    11221 attttttgcg ttttactctt attttggact attatgagac aaatgtaaga ctagactggc
    11281 cccctgaatc tccagacaac cagtatcact taaataagtg atagtcttaa tactagtttt
    11341 tagactagtc attggagaac agatgattga tgtcttaggg ccggagaaac gcagacggcg
    11401 taccacacag gaaaagatcg caattgttca gcagagcttt gaaccgggga tgacggtctc
    11461 cctcgttgcc cggcaacatg gtgtagcagc cagccagtta tttctctggc gtaagcaata
    11521 ccaggaagga agtcttactg ctgtcgccgc cggagaacag gttgttcctg cctctgaact
    11581 tgctgccgcc atgaagcaga ttaaagaact ccagcgcctg ctcggcaaga aaacgatgga
    11641 aaatgaactc ctcaaagaag ccgttgaata tggacgggca aaaaagtgga tagcgcacgc
    11701 gcccttattg cccggggatg gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt
    11761 gcgcagttgc acgtcattct cagacgaacc gatgactgga tggatggccg ccgcagtcgt
    11821 cacactgatg atacggatgt gcttctccgt atacaccatg ttatcggaga gctgcccacg
    11881 tatggttatc gtcgggtatg ggcgctgctt cgcagacagg cagaacttga tggtatgcct
    11941 gcgatcaatg ccaaacgtgt ttaccggatc atgcgccaga atgcgctgtt gcttgagcga
    12001 aaacctgctg taccgccatc gaaacgggca catacaggca gagtggccgt gaaagaaagc
    12061 aatcagcgat ggtgctctga cgggttcgag ttctgctgtg ataacggaga gagactgcgt
    12121 gtcacgttcg cgctggactg ctgtgatcgt gaggcactgc actgggcggt cactaccggc
    12181 ggcttcaaca gtgaaacagt acaggacgtc atgctgggag cggtggaacg ccgcttcggc
    12241 aacgatcttc cgtcgtctcc agtggagtgg ctgacggata atggttcatg ctaccgggct
    12301 aatgaaacac gccagttcgc ccggatgttg ggacttgaac cgaagaacac ggcggtgcgg
    12361 agtccggaga gtaacggaat agcagagagc ttcgtgaaaa cgataaagcg tgactacatc
    12421 agtatcatgc ccaaaccaga cgggttaacg gcagcaaaga accttgcaga ggcgttcgag
    12481 cattataacg aatggcatcc gcatagtgcg ctgggttatc gctcgccacg ggaatatctg
    12541 cggcagcggg cttgtaatgg gttaagtgat aacagatgtc tggaaatata ggggcaaatc
    12601 ca
//
