LOCUS       NZ_CP116990            12602 bp    DNA     circular CON 11-MAR-2024
DEFINITION  Escherichia coli strain MLI108K1 plasmid p12_MLI108-1, complete
            sequence.
ACCESSION   NZ_CP116990
VERSION     NZ_CP116990.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29553891
            Assembly: GCF_024700875.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12602)
  AUTHORS   Riediger,M. and Zautner,A.E.
  TITLE     Molecular epidemiology of Escherichia coli with resistance against
            third-generation cephalosporins isolated from stool samples of
            European soldiers with diarrhea on deployment in Western African
            Mali
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12602)
  AUTHORS   Riediger,M. and Zautner,A.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JAN-2023) Institute of Medical Microbiology and
            Hospital Hygiene, Medical Faculty, Otto-von-Guericke University
            Magdeburg, Leipziger Strasze 44, Magdeburg, Saxony-Anhalt 39120,
            Germany
REFERENCE   3  (bases 1 to 12602)
  AUTHORS   Zautner,A.E. and Riediger,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUL-2022) Institute of Medical Microbiology and
            Hospital Hygiene, Medical Faculty, Otto-von-Guericke University
            Magdeburg, Leipziger Strasze 44, Magdeburg, Saxony-Anhalt 39120,
            Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-JAN-2023
            Assembly Method :: trycycler v. 0.5.3; unicycler v. 0.5.0; skesa v.
            2.4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/11/2024 05:22:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,988
            CDSs (total)                      :: 4,863
            Genes (coding)                    :: 4,603
            CDSs (with protein)               :: 4,603
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 260
            CDSs (without protein)            :: 260
            Pseudo Genes (ambiguous residues) :: 0 of 260
            Pseudo Genes (frameshifted)       :: 83 of 260
            Pseudo Genes (incomplete)         :: 179 of 260
            Pseudo Genes (internal stop)      :: 53 of 260
            Pseudo Genes (multiple problems)  :: 51 of 260
            Pseudo Genes (short protein)      :: 3 of 260
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116990.1.
            Bacteria and source DNA available from Institute of Medical
            Microbiology and Hospital Hygiene Medical Faculty Otto-von-Guericke
            University Magdeburg 39120 Magdeburg, Germany.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MLI108K1"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p12_MLI108-1"
                     /geo_loc_name="Mali: Koulikoro"
                     /lat_lon="12.52 N 7.34 W"
                     /collection_date="2014-07-24"
                     /collected_by="Bundeswehr Medical Service"
     gene            63..197
                     /locus_tag="NL409_RS01620"
                     /old_locus_tag="NL409_001610"
     CDS             63..197
                     /locus_tag="NL409_RS01620"
                     /old_locus_tag="NL409_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072003336.1"
                     /translation="MHEPPVQSDRCVLSGNYRLESNPERHAKTPLAAAIGKQDVREMQ"
                     /name="replication initiation protein"
     gene            complement(627..995)
                     /locus_tag="NL409_RS01625"
                     /old_locus_tag="NL409_001615"
     CDS             complement(627..995)
                     /locus_tag="NL409_RS01625"
                     /old_locus_tag="NL409_001615"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032242306.1"
                     /translation="MFSYLKAMYHQSKIQAELKAQIHEQTTVNAICHHPESIEIIAVCS
                     TDAYYRKRKDAAFLTTCSVLMRTLKDESVPMVLRKTAWRLLNERYQRIKLNQAYRIENF
                     LLVADFEYALEEHDELAE"
                     /name="hypothetical protein"
     gene            1340..1576
                     /locus_tag="NL409_RS01630"
                     /old_locus_tag="NL409_001620"
     CDS             1340..1576
                     /locus_tag="NL409_RS01630"
                     /old_locus_tag="NL409_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_032242304.1"
                     /translation="MTEMERQLLSALESLQTSYEQQKQAWQDSYSSLQRMFETTSQALE
                     RNDRVCLNLSNQVNALHEQVERLSSDLSRLTRS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1721..2053
                     /locus_tag="NL409_RS01635"
                     /old_locus_tag="NL409_001625"
     CDS             1721..2053
                     /locus_tag="NL409_RS01635"
                     /old_locus_tag="NL409_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_032242302.1"
                     /translation="MSHAIEFIETTMFTRQIKQIATDDEIKELQKELIESPDKGDLIQK
                     TGGLRKIRMATGNQGKSGSARVIYFLATAEVIYLVMAYPKSTKDSITDAEKAELKKLTQ
                     RLKDEV"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2055..2339
                     /gene="nadS"
                     /locus_tag="NL409_RS01640"
                     /old_locus_tag="NL409_001630"
     CDS             2055..2339
                     /gene="nadS"
                     /locus_tag="NL409_RS01640"
                     /old_locus_tag="NL409_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_032242301.1"
                     /translation="MSFFDELKASLEEAVEIKNGTKAPARVTRYELADVRAIRENLNIT
                     QSEMAKVLGTSLDTIKSWETGRRNPTGLAAKVLATIQANPKFFDELAAH"
                     /name="nadS"
     gene            2538..3414
                     /locus_tag="NL409_RS01645"
                     /old_locus_tag="NL409_001635"
                     /pseudo=""
     CDS             2538..3414
                     /locus_tag="NL409_RS01645"
                     /old_locus_tag="NL409_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946055.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            3559..>3823
                     /locus_tag="NL409_RS01650"
                     /old_locus_tag="NL409_001640"
                     /pseudo=""
     CDS             3559..>3823
                     /locus_tag="NL409_RS01650"
                     /old_locus_tag="NL409_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /name="IS1-like element transposase"
     gene            complement(3916..5460)
                     /locus_tag="NL409_RS01655"
                     /old_locus_tag="NL409_001645"
     CDS             complement(3916..5460)
                     /locus_tag="NL409_RS01655"
                     /old_locus_tag="NL409_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="WP_201360865.1"
                     /translation="MKNYDVFYGGIFKTITDYKLLKIQKDIKNNMTALVSSFKWVGIGQ
                     LVKIATQLVSLFYLTHLIPPSSYGLLAMSSVVMNLASIFNDMGTGAAVIQRQEASIGFY
                     NYIYRINIFTGLFVMLLVIITSPLIVVYFNTPELYPVLFLLAFSFPISSLTITHKAKLE
                     KEMNFKVIIAIEIFSSLTGMVLAIVLANLGFGVYSLIFQTLTTISISTICFLKVSGLSL
                     SLRIKNISHSDKSGVIGFSGYLLSFNLINYFSRNLDSILIGRFFSSAILGVYSIAYRIM
                     LFPLQSLTLVFSRVFLPHLSKNINNTNNTNKQDYLNALKLILSLSAPLMLGLSATSFSF
                     TRIFFDPKWHLLGDLLHWLAPTAIIQSALSTTGSVFMAYGKTRWLFFLGCIGAFLMGLS
                     FIIGVHFDIKTLVYLYFFANLINSLPAFILLKNILKFSYIELVNVFFRTISPAFLMFLL
                     IKVWQHFSFFKNASIDFSFNVALGIFSYIVFYTLFNFNEVVRTGVIHKLRCMTKLK"
                     /name="lipopolysaccharide biosynthesis protein"
     gene            5619..6316
                     /locus_tag="NL409_RS01660"
                     /old_locus_tag="NL409_001650"
     CDS             join(5619..5868,5868..6316)
                     /locus_tag="NL409_RS01660"
                     /old_locus_tag="NL409_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_223368647.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEHWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            complement(6775..7758)
                     /locus_tag="NL409_RS01665"
                     /old_locus_tag="NL409_001655"
     CDS             complement(6775..7758)
                     /locus_tag="NL409_RS01665"
                     /old_locus_tag="NL409_001655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_151166972.1"
                     /translation="MKKKFSIIVPVYNVEKYLRECIQSVLDQDFNDYEIICVNDGSTDG
                     SLDIILSFDDQRIKVINQENKGLSVARNVAIESSTGEYIWFLDSDDKISHGSLKLIDKI
                     TNEKNYGIIFFNTHVFFDTPELKKKGWHDYKRSTFGEMDALTFFEKEMTNKKLIVSACC
                     YVFRRELLNGNAFIPHIYHEDNHFIATLIFDNYSTICLSLDEALYLRRVRNDSIMTSKK
                     SMRHIIGYSTCIDELTKYKKTKGIQNKSFDRFVNGMVLSLFSIAETIKTKEAMNYVARY
                     KKHPDLSFKDRALINYPLIKKTYLKLKNGKVFNYLRNGIINKKSNP"
                     /name="glycosyltransferase family 2 protein"
     gene            complement(7843..8211)
                     /locus_tag="NL409_RS01670"
                     /old_locus_tag="NL409_001660"
     CDS             complement(7843..8211)
                     /locus_tag="NL409_RS01670"
                     /old_locus_tag="NL409_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020936656.1"
                     /translation="MANFNPGPRTAIAAVRDSKNDIETWKKNGIRREEIVTVLNERYGL
                     NISLVAFDKALYRLRKSTDEPSLADVDSVSAAETNFSEASGPDNGFFLSGNDFMRAEEE
                     SQRVVESFRDSLSRKKGF"
                     /name="hypothetical protein"
     gene            complement(8242..9470)
                     /locus_tag="NL409_RS01675"
                     /old_locus_tag="NL409_001665"
     CDS             complement(join(8242..9155,9155..9470))
                     /locus_tag="NL409_RS01675"
                     /old_locus_tag="NL409_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="WP_088895425.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQ
                     LFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVEYG
                     RGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDVLLR
                     IHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVPPSKR
                     AHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQ
                     DVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGI
                     AESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACN
                     GLSDNRCLEI"
                     /name="IS3-like element IS2 family transposase"
     gene            complement(9930..10079)
                     /locus_tag="NL409_RS01680"
                     /old_locus_tag="NL409_001670"
     CDS             complement(9930..10079)
                     /locus_tag="NL409_RS01680"
                     /old_locus_tag="NL409_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_167776965.1"
                     /translation="MPSEHDEQLDSTDTQLPGEYDLFDLMAQVTEENLHALVDFGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            11164..11481
                     /locus_tag="NL409_RS01685"
                     /old_locus_tag="NL409_001675"
     CDS             11164..11481
                     /locus_tag="NL409_RS01685"
                     /old_locus_tag="NL409_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="WP_032242326.1"
                     /translation="MKKIFILSAALIMAGCATKQYPQAVNVTGPESSAMDCKAVALEIA
                     KTRSVQQEIEETGEFDGRTVLGFLGDFGIGNGMAKGEARKKAANRLNQLEALKAAKCAD
                     Q"
                     /name="lipoprotein"
     Typing          complement(10115..10288)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.143"
                     /coverage="100.0"
                     /evalue="5.58e-78"
     Typing          2..387
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.804"
                     /coverage="99.0"
                     /evalue="3.95e-104"
     Typing          10255..10763
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.51"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          12295..12602
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.068"
                     /coverage="90.0"
                     /evalue="6.02e-88"
ORIGIN
        1 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact
       61 gtatgcacga accccccgtt cagtccgacc gctgcgtctt gtccggtaac tatcgtcttg
      121 agtccaatcc ggaaagacac gcaaaaacgc cactggcagc ggccattggt aaacaggatg
      181 tgcgagagat gcagtaagag ttcttgaagt gatggcctga gtgcggctac actgaaagga
      241 cagatttggt gacatgtctc gcacaagact gttaccgcgg ttagaaaatc tgccaggatt
      301 agctaacctt cgaaaaacca cctgccaggg tggttttttc gtttacagag caagagatta
      361 cgacgcaaca gaaaggatct caagaagttc cttgctgtat tgcgggtccg cttggaaaaa
      421 ggaaattatc ctacgttaag cgttttaagc taacgggtga tttcgttcca ttgagcgcga
      481 acccctgacc gtaagggatg tacgaaatga tccggctgtc cgttaatggt tttgcttact
      541 ttcggacatg ttgtctacat gctgtacgta atcggacgtt taagtcacat gcacgttaac
      601 tgtcggtatt catcgcctca ctagtactac tctgctagtt catcatgttc ttcgagagca
      661 tattcaaaat cggcaacaag caagaaattt tctatcctat aagcctgatt taacttaatt
      721 cgttggtatc tttcgttcaa taatcgccat gctgttttcc gtagtaccat cggtacggat
      781 tcatctttta aagtacgcat gagcacagaa caggttgtca gaaaagcagc gtccttacgt
      841 tttcggtaat acgcatcagt cgaacaaacc gcaatgatct cgattgattc agggtgatga
      901 cagatagcat taaccgtagt ttgttcatgt atctgtgcct tgagttcagc ctgaattttt
      961 gattgatgat acatagcctt gagatagcta aacatgattt ccccatcaaa cagtaagtcg
     1021 tgtcaattcc tagagttaac acatttctga tcatgactca cacgttacgt tcagtcggta
     1081 gccatctgtg caaaaaatgc gcttagcgta ggataatttc ctttttccaa gcggacccca
     1141 gagcggcgca ggggtagcga tttacaagga cgacaagcag aagaacacct atctggtggt
     1201 actgccgaag aaagcgcatg gcaccgagac atacacatcg acagccggaa acaccgtgat
     1261 gaagtacacc gcgaaataac cccgtcagac accacagaaa cgccacagca cgtttttatc
     1321 actgaggaca ggaaaaacca tgaccgagat ggaaaggcag cttctgagcg ccttagaaag
     1381 cttacaaaca agctacgaac agcagaagca ggcgtggcag gacagttaca gcagcttgca
     1441 acgcatgttc gagactacct cacaggcact ggagcgcaac gacagggtat gtctgaactt
     1501 gagcaatcag gtgaacgcct tgcacgaaca ggtcgaacgc ttgagcagcg atctgagccg
     1561 attaacacgc tcgtaaagca gcaggaacgg cagttacagc aggagaagca agcaacccgc
     1621 tatcgaggtc agtccatgta acgtctgatg gcacagaacg cggcttttca gaacacgcct
     1681 cttggtgtac attgtacatc aaaatataca ggagacagcc gtgagtcacg cgattgagtt
     1741 tatcgagaca acgatgttta cccgtcagat aaagcaaatc gccacggatg atgagatcaa
     1801 agagcttcag aaagagctta tcgagtcacc ggacaaaggt gacctcatcc agaaaacggg
     1861 cggtctgcga aagatcagga tggcgacggg gaatcaggga aagagcggca gtgcgcgggt
     1921 gatttacttc cttgccaccg cagaggtgat ttatctggtg atggcctacc ctaaaagcac
     1981 caaagacagc atcacagacg cagagaaagc agaactgaag aagctgaccc aacgactgaa
     2041 agacgaggtg taagatgagt ttttttgacg agctgaaagc ctctctggaa gaggccgttg
     2101 aaatcaaaaa cggaaccaaa gcccccgccc gtgtgacccg ctacgagctg gcagacgtca
     2161 gggctatcag agaaaacctg aatattactc agagcgaaat ggcgaaagtg ctgggaacca
     2221 gcctcgatac catcaaaagc tgggaaaccg gacgccgtaa cccaaccgga ctggcagcaa
     2281 aagtgctggc caccattcag gctaacccaa agttttttga tgaactggcg gcgcattaag
     2341 ctgatgtgac agaagaaatt cgacaggcga ggccgttcgc gtgtaacttt aacggcaaca
     2401 gaaaaaacaa agccccgata atcatgattt caacttggcg gaagagacat gataaatcag
     2461 ggctaggtat aaaacctaag aaggatatta gcacatgaac cgtgccagac aacagcccgc
     2521 cataagttca gggcacattg cgtaacgcag cactcgaact tttcaacgac cgcttgccgc
     2581 ataaaccgta cttctcggat gatttacact ttggtgtccg catcgcaggg aaagagcgcg
     2641 ctattctggc taaatacatc cagtttaatc agcctcacgc catgttctgg ctgggatttg
     2701 acgttgaccg catcggcgca gccattgact ggagcgaccg caacgccccc gcgccgacac
     2761 tgaccatcac caaccccgaa aacggacacg cgcacctgct gtacgcactg aaaacctcga
     2821 tacgcaccgc gccagacgga aaaatgaaac cgttacgtta cgccgctgct gtagagagcg
     2881 cgctacgtaa aaaattgggc gctgacacgg ggtattcggg gctaatttgc aagaatccga
     2941 accacagtca ctggaaaatt gcccgtatgg cagcctgaac tctacacgct ggactggtta
     3001 gccgaatcac tcgacctgaa cgctgcgaac gataaaaaaa tcgtcgccga ttatggctta
     3061 ggtcgtaact gcatactgtt tgataaaacc agaaagtggg catatcgcgc tatccgtcag
     3121 ggctggcctg agtacgaaca gtggctacaa gcctgccatg aacgcgcctg cgcttacaat
     3181 ctgcaatttg cagtgccttt gaatgagaat gaagtaacgg gaatagcgaa aagtatctgc
     3241 aagtggacat ttttgaaatt taataagcaa aatttttcac ttatacaagc caaaagagga
     3301 aaagttggtg gtgaaaaatc atcaggagga ggtaggctgt ctctagggga gccttgggtt
     3361 gctttaggta ttagtcgcgc tacatttttt agaaaaagga aaatttataa ttaagattca
     3421 gtattttaca tactgaatct taattattga atcaccatta cttaagtgtt gatagtgttt
     3481 tttttccagc agtaacaact gtttgtgatg atgccaactt gctggtttag tgtctaatgg
     3541 tgtttttgag gtgctcccgt ggcttccatt tccatcagtt atccctcctg ttcagctact
     3601 gaaggcgtgg tgcgtaacgg taaaagcact accggacatc aacgctatct ctgctctcac
     3661 tgtcgtaaaa catagcaagc aactacagtt cacttacacc gcctctgagc ccggtacaca
     3721 ccagaaaatc attgatatgg ccatgaatgg cgttggatgc cgggccacat cccgcattat
     3781 gggcactgtc ctcaacacga ttttacgaca cttaaaaaaa ttaggccgaa gtcggtatcg
     3841 ggcattatct gaacataaaa cacaagcagt aagttggagt cattaccaat tcatttaatg
     3901 aatttaataa atgaatcact ttaacttggt catgcatctt aacttatgaa ttactcctgt
     3961 cctaacaacc tcattgaaat taaacaaggt ataaaaaaca atataactaa aaatacccaa
     4021 tgcgacatta aaagaaaaat caatgctggc atttttgaaa aaggaaaaat gctgccatac
     4081 tttaataagt aagaacatca aaaaagctgg ggagatagtt ctaaaaaaca cgtttaccaa
     4141 ttcaatgtaa ctaaacttca agatattctt aagtaaaata aatgccggga gtgaattaat
     4201 taagtttgca aaaaaataaa ggtagaccaa tgtttttata tcgaaatgaa cacctataat
     4261 gaaagacaac cccatcaaaa aagcgccaat gcaacctaag aaaaacaacc accttgtttt
     4321 tccgtaggcc ataaaaacag aacctgttgt actcaaggct gattggataa tcgctgtagg
     4381 tgccaaccaa tgcaataagt ctccaagtag gtgccactta ggatcaaaga aaatcctcgt
     4441 aaaagaaaaa gatgtagcag ataaacctag catcagtggc gcgcttaatg atagtataag
     4501 ttttaatgca tttaagtaat cttgtttatt agtgttatta gtgttattta tgtttttgct
     4561 aaggtgtggc aagaaaactc tagaaaaaac aagcgtcagg ctctgcaacg gaaataacat
     4621 aatgcggtag gcaatcgaat agaccccaag tattgcggat gagaaaaaac ggcctattaa
     4681 aatagaatca agattcctgg aaaaataatt tattaaatta aaagacaata aataaccact
     4741 aaaccctata actcctgact tgtcactatg gctgatattt ttaattctca atgataaact
     4801 caacccggaa accttcaaaa aacatattgt ggatattgat attgttgtca gtgtttgaaa
     4861 aatcaaacta taaacaccaa agcctaaatt agctaaaact attgccagca ccattcctgt
     4921 aaggctagag aaaatttcaa ttgcaatgat gactttaaag ttcatttctt tttctaattt
     4981 tgctttgtga gttattgtta agctggaaat tggaaagcta aaagccaata gaaatagaac
     5041 cgggtatagc tcgggtgtgt taaagtacac gacaattaat ggggatgtta ttattacaag
     5101 cagcataaca aacaacccag taaaaatgtt tatcctgtat atgtaattat aaaaaccgat
     5161 cgacgcctct tgtcgctgaa ttactgccgc cccagttccc atgtcattga atatgcttgc
     5221 taaattcata accacagatg acattgctaa tagtccatag ctcgatgggg ggattaagtg
     5281 cgtcaagtag aataacgaaa ccaattgtgt tgctattttt acaagttgtc caatgccaac
     5341 ccatttaaaa ctactgacta gtgcagtcat attgttttta atatcctttt gaattttcaa
     5401 taatttataa tccgtgatgg ttttaaagat accaccataa aagacatcat aattcttcaa
     5461 gctatcatct acgttgtaga ttccgattta gctaaacttt aggactttat aagttcgtat
     5521 cccgtatgat tataaggcat tatgatcatc tccaccaata gtgggtaatg ctgccaactt
     5581 actgatttag tgtatgatgg tgattttaag gtgcttgcgt ggcttccatt tccatcagat
     5641 gtccttcctg ctccgctact gaaggcgtgg tgcgtaacgg caaaagcact gccggacatc
     5701 agcgctatct ctgctctcat tgccgtaaaa catggcaact acagttcact tacaccgcct
     5761 ctcagcccgg tacgcaccag aaaatcattg atatggccat gaatggcgtc ggatgtcgcg
     5821 ccagtgcacg cattatgggc gttggcctca acacggtttt acgtcactta aaaaactcag
     5881 gccgcagtcg gtaacctcgc gcatacaacc gggaagtgat gtgattgtct gcgctgaaat
     5941 ggacgaacat tggggctacg tcggtgctaa atcacgtcag cgctggctgt tttacgcgta
     6001 tgacaggata cggaggacgg ttgtggcgca cgtcttcggt gaacgcactc tggccacact
     6061 ggagcgtctt ctgagcctgc tgtcggcctt tgaggtcgtg gtatggatga cggatggctg
     6121 gccgctgtat gaatcacgcc tgaagggaaa gctgcacgtt atcagcaagc gatatacgca
     6181 gcgcattgag cgacataatc tgaatctgag acaacatctg gcaaggctgg gacggaagtc
     6241 actgtcgttc tcaaaatcgg tggagctgca tgacaaggtc atcgggcatt atctgaacat
     6301 aaaacactat cagtaagttg gagtcattac ccggtctgcg ttgtcgggaa gatgcgtgat
     6361 ctggggttag tttggatata gagaattatt gtacggtaag cctgtttttt gaacagactt
     6421 acctatagca atagggtgta ctcccgcgct atacggcatt ttcagaggtt tgaccttaat
     6481 cggataacct aaatgcgata ctgcaagagc gacaacttaa aagcgatagc cactgacgca
     6541 aaatcaaccc cttacgaacc aagattggtt aaataatgcg cttaacgtac aaaaatttcc
     6601 gatctccaaa ctgaccccga tctgatcctt caactcagca aaagttcgat ttattcaaca
     6661 aagccccgga aagcgtgcgt atcgcctgaa aacccaaccc gtcacgggga tacctatccg
     6721 aaatcttatt tattcaacaa agaaaaacct ttattaatta tcgctttgct tttattaagg
     6781 gttgcttttt ttattaataa tgccattcct taggtaatta aacaccttcc cgttttttag
     6841 ttttaaataa gtctttttaa ttaatgggta attaattaat gctctatctt taaatgatag
     6901 gtcggggtgt tttttatatc tcgccacata gttcattgct tcctttgttt ttattgtttc
     6961 cgctatagag aataacgaca aaaccattcc attcacaaat ctatcaaagc tcttgttttg
     7021 tatgcctttt gtttttttat acttcgtaag ctcatcgatg catgttgaat atccaatgat
     7081 gtgcctcatt gattttttag acgtcattat ggaatcattt cttaccctcc taagatataa
     7141 cgcctcatcc aacgagaggc atatagttga ataattgtca aaaatcaggg ttgctataaa
     7201 gtgattatcc tcatgatata tatgcggaat gaatgcattg ccatttaata attcccttct
     7261 aaaaacatag cagcatgctg acactattag ttttttgttc gtcatttctt tttcaaagaa
     7321 agttaatgca tccatttcac caaatgttga acgcttataa tcatgccagc cttttttttt
     7381 cagttctgga gtgtcaaaaa aaacatgtgt attaaagaaa ataatcccat aatttttttc
     7441 attggttatc ttgtctatta actttagact tccatgtgat attttatcat cactatctaa
     7501 aaaccatata tattcaccag ttgatgattc aatggcgaca tttcttgcca ccgacaggcc
     7561 tttattttcc tgatttatta ctttgattct ttggtcatca aatgacagta tgatatctag
     7621 actgccatct gttgaaccat catttacaca gattatctca tagtcgttaa aatcctgatc
     7681 taatactgat tgaatacatt cccttaagta tttttcaacg ttatatacag gtactattat
     7741 cgaaaatttt tttttcacag taatctccaa acaatattta gttatactaa catcaagttg
     7801 ttatttatgg aatgcgattt atcaacggct ttgttgaata aatcagaatc ctttcttcct
     7861 gctaaggcta tctctgaatg actcaaccac tctttgagat tcttcttctg ctcgcatgaa
     7921 gtcattcccg gacagaaaaa aaccattgtc aggcccactg gcttcagaga aattcgtttc
     7981 tgcagcagac acagaatcca catcagcaag agacggttca tctgtacttt ttctcaatct
     8041 gtaaagagcc ttatcaaacg caaccagtga gatattcagg ccgtaacgtt cattcagcac
     8101 agtcacaatc tcttccctac gaataccatt tttcttccag gtttctatat cgttcttgga
     8161 atccctgaca gctgcaatgg ctgttcttgg accagggtta aaattcgcca tataccgccc
     8221 ataatgtctt tggatttgcc cctatatttc cagacatctg ttatcactta acccattaca
     8281 agcccgctgc cgcagatatt cccgtggcga gcgataaccc agcgcactat gcggatgcca
     8341 ttcgttataa tgctcgaacg cctctgcaag gttctttgct gccgttaacc cgtctggttt
     8401 gggcatgata ctgatgtagt cacgctttat cgttttcacg aagctctctg ctattccgtt
     8461 actctccgga ctccgcaccg ccgtgttctt cggttcaagt cccaacatcc gggcgaactg
     8521 gcgtgtttca ttagcccggt agcatgaacc attatccgtc agccactcca ctggagacga
     8581 cggaagatcg ttgccgaagc ggcgttccac cgctcccagc atgacgtcct gtactgtttc
     8641 actgttgaag ccgccggtag tgaccgccca gtgcagtgcc tcacgatcac agcagtccag
     8701 cgcgaacgtg acacgcagtc tctctccgtt atcacagcag aactcgaacc cgtcagagca
     8761 ccatcgctga ttgctttctt tcacggccac tctgcctgta tgtgcccgtt tcgatggcgg
     8821 tacagcaggt tttcgctcaa gcaacagcgc attctggcgc atgatccggt aaacacgttt
     8881 ggcattgatc gcaggcatac catcaagttc tgcctgtctg cgaagcagcg cccatacccg
     8941 acgataacca tacgtgggca gctctccgat aacatggtgt atacggagaa gcacatccgt
     9001 atcatcagtg tgacgactgc ggcggccatc catccagtca tcggttcgtc tgagaatgac
     9061 gtgcaactgc gcacgcgaca cccggagaca acggctgact aagcttactc cccatccccg
     9121 ggcaataagg gcgcgtgcgc tatccacttt tttgcccgtc catattcaac ggcttctttg
     9181 aggagttcat tttccatcgt tttcttgccg agcaggcgct ggagttcttt aatctgcttc
     9241 atggcggcag caagttcaga ggcaggaaca acctgttctc cggcggcgac agcagtaaga
     9301 cttccttcct ggtattgctt acgccagaga aataactggc tggctgctac accatgttgc
     9361 cgggcaacga gggagaccgt catccccggt tcaaagctct gctgaacaat tgcgatcttt
     9421 tcctgtgtgg tacgccgtct gcgtttctcc ggccctaaga catcaatcat ctgttctcca
     9481 atgactagtc taaaaactag tattaagact atcacttatt taagtgatac tggttgtctg
     9541 gagattcagg gggccagtct agtcttacat ttgtctcata atagtccaaa ataagagtaa
     9601 aacgcaaaaa atgtcttaca attgtcttat ttttgtcctt tccggttcat ggatacatga
     9661 accgcctgat gtggaacgct ttttatcgat ctcatgcgct gccaaagata gattcattct
     9721 ttaaaagtca ggttacatta tcccttaaat ggtcatacac catacgcaac cgcgattttc
     9781 atcgcaattg catgaattaa aagataaaaa ctgaagtccc cgtttattta tgtaatacgc
     9841 aacgtattac aaccacctgt aattacatga aaataaagaa ataagatgta ataccggcgt
     9901 attaccacgt acgcgggtaa gcgaaaaagc tataacgctt cccggccgac aggcggccca
     9961 aaatccacca gggcatgcag gttctcttca gtaacctgcg ccattaaatc aaaaaggtca
    10021 tattcgccgg gaagctgcgt atcagtcgaa tccagttgtt catcatgttc agatggcatg
    10081 gggtgatatc cagtagctga gaggttgtta gagggtttcg gggcgcagcc ctgaaccagt
    10141 cacgtagcgc tagcggagtg tatactggct tactatgttg gcactgatga gggagtcagt
    10201 gaagtgcttc atgtggcagg acaaaaaagg cggcaccggt gcgtcagcag aatatgtgat
    10261 acaggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc
    10321 gagcggaaat ggcttacgaa cggggcggag atgtcctgga agatgccagg gaaatactta
    10381 acagggaagc gatagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa
    10441 gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata
    10501 ccaggcgttt ccccctggcg gctccctcgt gcgctctcct gttcctgcct ttcggtttac
    10561 cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg
    10621 ggaaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgactgct
    10681 gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacacgac aaagcgccac
    10741 tggcagcagc cactggtaac aggattagca gagcgagtta atgtaggtgg tgctatagag
    10801 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
    10861 ctgctgaagc cagttaccag gttaagcagt tccccaactg acttaacctt cgaaaaacca
    10921 cctccccagg tggttttttc gttttcagag caagagatta cgcgtagaaa aaaaggaact
    10981 caagaagatc ctttctatgc tcaaccaccg catggcccat agccgcgctg gccgttgccg
    11041 gtcagctcca gaatcctcga ccaatatgga accttgcccc tcccaaaatt caactaaaaa
    11101 accctcattt cctgccgata aaatgggtat ctcttaacta tgcctaaata aataaggata
    11161 attttgaaaa aaatattcat cctatcggca gcgctgatca tggcaggatg cgcaacaaag
    11221 caatatccac aggccgtgaa cgtcaccggt ccagaatctt ccgcaatgga ttgcaaagcg
    11281 gtagctctgg aaatcgccaa aacgcgcagt gtacaacaag aaattgagga aaccggcgaa
    11341 tttgacgggc gcaccgtatt aggtttcctg ggtgattttg ggataggaaa tggcatggca
    11401 aaaggcgaag cccgcaaaaa agcagctaac cggctaaatc agctggaagc cctgaaggct
    11461 gcaaagtgtg cagatcaata aggtacaaaa aagccctcgg ggcttttttt gtacctgcca
    11521 atcagctgaa tttaccataa catccgttgc gtgcaccatc tcagacgccc tcagggcatt
    11581 tctgcgccct cctgcctatt tccggcatct ttccctgtcc atatcgtgaa aaaacgcgcc
    11641 tgatgatttc ttcgcgtctg catggctatg cacacgaacg catggacgtc attttagcgg
    11701 ctaaaatccg gcgagcgccc tgaaagccac cacgaacccc accgataacg gtgaaagggt
    11761 gcgcagttta ctgtgcgacc gtgaggcgtt ggccaggccg tcaggcatgg caggatttat
    11821 tgctaagggg gtcggattta acctggatta atcagcacac agtgatgaac gggattcgga
    11881 aggggcaccc ttgcggcgcg gcgatgtata catcgctaac gtgccttggt catttattgc
    11941 aagaagggag aggtagcatt ttgatagcaa aaaagtagca aaaggatagc aaccgaaaga
    12001 ccagaagact ctggagatag caaaaaagta gcaaaatggt agcaaaaaga tagcaaacat
    12061 gcgggagcta accaggagtc tttagcgggc cggcggtact ccaaaaacac attttgtccc
    12121 gctgttttgt gaataaccca gcatagctgt gggcacaaaa catttcctgt ccgctgcttt
    12181 aacctttgta atccaggaac gtcaggtgac gtcactttgc ggcgttagcc ctatcctgca
    12241 tcctatgaaa aaatgcagtt acggagtgta aggtgccgga cgtgaggccg aagatattcc
    12301 gcttcctcgc tcactgactc gctacgctcg gtcgttcggc tgcggcgagc ggacggagaa
    12361 tattcacaaa ggcatatggc gatgaagacg agataaagcg agggaggaaa ggccgcggcg
    12421 ctgccggttt tccataggct ccgcccccct gacaagcatc acagaatctg acgctcaaat
    12481 cagtggtggc gaaacccgac aggactataa agatcccagg cgtttccccc tggtagctcc
    12541 ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg
    12601 cg
//
