LOCUS       NZ_CP116966             5185 bp    DNA     circular CON 10-MAR-2024
DEFINITION  Enterococcus faecalis strain DM86 plasmid unnamed1, complete
            sequence.
ACCESSION   NZ_CP116966
VERSION     NZ_CP116966.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32953418
            Assembly: GCF_028532705.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Shen,W. and Cai,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2023) Clinical Microbiology Laboratory, The Second
            Affiliated Hospital of Zhejiang University School of Medicine,
            Zhejiang University, 88 Jiefang Road, Hangzhou 310009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v.0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/10/2024 16:14:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,086
            CDSs (total)                      :: 3,005
            Genes (coding)                    :: 2,954
            CDSs (with protein)               :: 2,954
            Genes (RNA)                       :: 81
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 65
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 51
            CDSs (without protein)            :: 51
            Pseudo Genes (ambiguous residues) :: 0 of 51
            Pseudo Genes (frameshifted)       :: 18 of 51
            Pseudo Genes (incomplete)         :: 29 of 51
            Pseudo Genes (internal stop)      :: 12 of 51
            Pseudo Genes (multiple problems)  :: 6 of 51
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116966.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="DM86"
                     /isolation_source="duck"
                     /db_xref="taxon:1351"
                     /plasmid="unnamed1"
                     /geo_loc_name="China"
                     /collection_date="2021"
     gene            441..527
                     /locus_tag="PO880_RS15355"
                     /old_locus_tag="PO880_15355"
     CDS             441..527
                     /locus_tag="PO880_RS15355"
                     /old_locus_tag="PO880_15355"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107196852.1"
                     /translation="MIESLISLIIAPLFVGLVLLLVDRWLDD"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(1336..3015)
                     /gene="mobV"
                     /locus_tag="PO880_RS15360"
                     /old_locus_tag="PO880_15360"
     CDS             complement(1336..3015)
                     /gene="mobV"
                     /locus_tag="PO880_RS15360"
                     /old_locus_tag="PO880_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401964.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_104659816.1"
                     /translation="MSKIVARMEKMKVGNLSGIQRHNQRETDNHSNPDIDIEKSHLNYD
                     LVNPGSINYREKIKQIIESQRISKRAVRKDAVLVNEWIITSDTAFFQENTDTQAFFTDV
                     VAYFSDRCGRQNVAYATVHLDETTPHMHLGIVPMYEGRLSSKQVFSRQSLLEIQEELPN
                     YLKERGYAIERGLRGSPQKHLSVKEYKDVQKEIQVSKQELANQEQILVEKERELHSYEQ
                     AIFRIDRLLEEKNEEVKGKVQAMNQLETKRNEIINSLQTTVLPDLANIKEKKFSFNGLK
                     YVLSASDLSAIKSITKDFETLRTQNKHLKIENNTLAQKNKELTQVNHEQELQITDLAYE
                     LTLKKEQLTAFEEKQLNYDQSEQIKQELDRTTQKVSVLETENKRLKDKITDLTYDFSEL
                     NEKYQEIKLKFDGLTTYLHERLGQAKETILFTIQQGVKYLQNAPKLKKSLERYSKDRTY
                     LIDEKGTLFLPPDTGSKGVTMSLNPHFYPLKAPFYNRETGCYEFVGEYMANGRSRTNTL
                     QLSEEQVLEKKAFTFEQSTDKKAWQQSKKYSRDMSKGRGLSR"
                     /name="mobV"
     gene            complement(3794..4090)
                     /locus_tag="PO880_RS15365"
                     /old_locus_tag="PO880_15365"
     CDS             complement(3794..4090)
                     /locus_tag="PO880_RS15365"
                     /old_locus_tag="PO880_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002404941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002404941.1"
                     /translation="MGLMTGYNMTKEEYEITQAVLTRLDKFESELESLLLDLEPLDLSR
                     LDTVVREETLRMFNYLSRSYILLLDTVAYKDTLEEDISSGIVKIDGQPLDEEE"
                     /name="hypothetical protein"
     gene            complement(4218..4949)
                     /locus_tag="PO880_RS15370"
                     /old_locus_tag="PO880_15370"
     CDS             complement(4218..4949)
                     /locus_tag="PO880_RS15370"
                     /old_locus_tag="PO880_15370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360724.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_025188181.1"
                     /translation="MNEKSELAVKYQNELNLVALKQFNAKEMNLFFALCARMKDKGLDS
                     IIFSFEELKELSDYKMTAIKAFTNDLDSLYSKMLQLTYRNEYDDDGSFRRFVLFTGFDV
                     NVKKQTVEVSINPKLEGILNGLTTEFSRFELSAFTAIRSTYAKTLFRLLMQYRSTGYYT
                     VSIDEFKEIMDIPKSYTQMGQIDQRVIKPAMKELNNYFENLELTKIKAKKGNKIAKLEF
                     TFTGLKTNKPSITMHDWVNGE"
                     /name="replication initiation protein"
     Typing          complement(1340..3090)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.918"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gccacgtggc acacagaccg cctaaaaagg ttttaaaaaa tatataaaaa atatataaaa
       61 ataaacgcgt gcgcgtgcga ggaaatttcc cgcctaaaaa ttcactcttt tttctacaaa
      121 caggttcgcg atatttacga aactgtcact aaaaaattga aaaacaaaaa aaggagtgag
      181 cgaaattctt cgctcacatt atgcttgcag cgttcacttc gttcactcgc taaaatcaaa
      241 atcaataccg ttgacaaggt aactcatttg gttaagctct atttgcttgt ctgaagctct
      301 ttaagacgtg tttagtggtt tcaggtaatc ttgttaagat tgcgccttgc aggcggtcag
      361 ggcaagccct aacaacctcc acaagaaatt cttcttgtga tatacttgtt ttggcaactg
      421 ccccgaaagg gggtgtaaac atgattgaaa gcttaatttc gttgattatc gccccacttt
      481 ttgtgggttt agtacttttg ctagttgatc gttggctaga cgattagcag ataatctatg
      541 cagttgcttt tctagctcca acgctgaaat cgcaacagtg taaaaaaagc cctcctactg
      601 agaataggag ggctttttgg tgtaaacatg attttgctta atttcgcttc gcacttacac
      661 aataacattt tttttctgta ttttcaaacg tcagattaga ttacgcaaaa cgtcaattag
      721 agaaatacca tatcgaaata gttagtatta aacatagtaa tactatgagg atagacaata
      781 ttttactttt tggactatct tcaaagcttt tattttctgg tctgtaagtg ggattcacta
      841 ctttcgcgtt gtaatccatt gtacttccca aatatacttc tgaaggaatt aaaaaaaata
      901 ataaaattcc gtaaaaaata attatcaata tgggcaaatc aatattaact aaaaaagaag
      961 ttatatagct taaaactaaa ggaatagtaa tataaaatat attttttatt agatattttt
     1021 tcataaaata acctcaaatt tagcaactat tttgtccatc ttgtatgcat tgggatacaa
     1081 aatccaccgg ctttatgata gcctcctgca gtccacccgc cttttttcca ataaaatcct
     1141 ggctgacact ctatagaatc ccactctcta gcacttgctt ctgtagttga acttaaagca
     1201 acagagataa aagacacacc taaaattaga actagcaact ttttcataat gattacatca
     1261 ctttttttta atttttatta atatatttta atttaactgt ttttatgtgt caataattta
     1321 tcaattaaat tatttttaac gagataaacc tcgtcctttt gacatatctc ttgaatattt
     1381 tttactctgt tgccaagcct ttttatctgt cgactgttca aacgtaaaag ctttcttctc
     1441 tagcacttgt tcttcagaca gctgtaaagt attagttcgt gaacggccat ttgccatata
     1501 ctcgccgaca aactcataac accctgtttc tctgttgtaa aacggggctt ttagggggta
     1561 gaaatgtggg tttaaggaca ttgttacccc ttttgacccc gtatctggtg gtaaaaacaa
     1621 tgtccctttt tcgtcaatga ggtaggttct gtccttggaa tatcgctcca gggatttttt
     1681 taatttaggc gcgttctgaa ggtacttcac tccttgctga atggtaaata aaatggtttc
     1741 tttggcctgt cctagtctct catgaaggta agtggttaac ccgtcaaact ttagttttat
     1801 ctcttgatat ttttcgttta gttcagaaaa gtcgtaggtt aagtcagtaa ttttatcttt
     1861 taaccgctta ttctctgttt ctaacacaga aactttttgt gttgttctat caagttcttg
     1921 tttgatttgt tcgctctggt catagtttag ttgtttttct tcaaacgctg ttaattgttc
     1981 ttttttcagg gttaattcat aggctaagtc cgttatttgt aattcttgtt catgatttac
     2041 ttgtgtcaat tctttatttt tctgcgccaa ggtattgttt tcgattttaa ggtgcttatt
     2101 ttgcgttcta agcgtttcaa aatctttcgt aatacttttt attgccgaaa ggtcagaagc
     2161 gcttagaacg tattttaaac cgttaaaact aaatttcttt tctttgatat ttgctaaatc
     2221 aggtaaaacg gttgtttgta gagagttaat gatttcgttt cgcttcgttt ccagttggtt
     2281 catggcttgg actttgccct ttacttcttc gttcttctct tcaagaagac ggtcaatacg
     2341 gaagattgcc tgttcatagg aatggagttc tctttctttt tctaccagaa tttgttcctg
     2401 attcgcgagt tcttgtttag aaacctgtat ttctttttga acgtctttgt attctttgac
     2461 agataaatgc ttttgcggac tcccacggag tccacgctca atagcgtagc cccgttcttt
     2521 caaatagttt ggcagttctt cctgaatctc taacaaactt tgccgactaa acacctgttt
     2581 actgctcaat cgcccttcgt acataggcac aattcctaag tgcatatggg gcgtggtttc
     2641 gtctaaatgt accgtggcat aggcgacatt ttgtcgaccg cagcgatcag agaaatacgc
     2701 gacaacatcg gtaaaaaatg cttgtgtgtc tgtattctct tggaaaaagg cggtatcact
     2761 agtgattatc cattcgttca caaggactgc gtcttttcta accgctcgtt tactgattcg
     2821 ttggctctca atgatttgtt tgattttctc ccgataattg attgaaccag gattgactaa
     2881 gtcataattc aagtgagatt tctcaatatc aatatcagga ttggaatgat tatcggtttc
     2941 tcgttgatta tgccgttgga taccgcttaa gtttccaact ttcattttct ccatacgtgc
     3001 gacaattttt gacatgagcg ctcccccccc ttttttttct tgtggcgttc tttgtctttg
     3061 gatttcggca gcacttccaa gtaaagtata acgcactata ctttacttcg taaaggtgcg
     3121 ctctgccgag ggctgtcggc tagtactcgg aaggtcagcc tttaggcttc ccaatctcgc
     3181 acttagccga ttgcctacgg tcaaactttc catttctgct aaggcagaaa ctccaaattc
     3241 ccctccgtca aaaaaccgtt taaataacga ctgcctgaaa ttagtgaacg tgctagcttt
     3301 ctgctagccg gtgctagtaa tgtgctaatt tggtgctaaa aacctgctag tcactgctag
     3361 gaatctgcta gtttgggtgc tagaaacctg ctagtgagtg ctaaaaatgt gctagtttac
     3421 ctgaaagtac ctgctagtaa agtgctagcg aagaacgaaa gtgtacccca cgggaaagcc
     3481 ttgcactgca aggcgtccca ccttgtgtgg gacccagttt cccttatgct cttccgaaac
     3541 acatgaaaca attaagtaaa tttcaagaac aaaagacaaa aaataacaac agtggggatt
     3601 gggggaaccc caaaaatttg aatgacacct accaaaaata ctggtaggtg tcattcaaaa
     3661 tatcgcaaca tggtaccatg ttgctctgct tgcaaaaaga aagcctaccc caaaatggaa
     3721 gtagactaat cttgatattt ttcgagtgta ccccgaatat ctaatacttt tttataacaa
     3781 tcagacctag tctttattct tcttcgtcta gaggctgacc gtcaattttc acgatcccac
     3841 tggaaatatc ttcttctagc gtatctttgt aggcgaccgt atcaagaagt agaatgtacg
     3901 accgacttag ataattaaac attcttagcg tttcttctcg gacgacagta tctaatcgtg
     3961 ataggtcgag tggctctaaa tcaagtaata atgattctag ttcactttca aacttatcta
     4021 gtcgagtcag tacagcctgt gtgatttcgt actcctcttt tgtcatgttg tatcctgtca
     4081 ttaatcccat gtatatgacc tccaaaataa tttattttag aagtcctcac gcaaatatgc
     4141 taaaataatc tagcagtcgt agtggattgc gtgaggaact tgctaacttg cttaatgtga
     4201 gggggcaagt tcttttttta ttctccgttt acccagtcat gcatagttat gcttggtttg
     4261 ttggttttca gaccagtaaa cgtaaattct agtttggcaa tcttattgcc ttttttggct
     4321 ttgatttttg ttaattctaa attctcaaag taattattga gttctttcat tgcaggtttt
     4381 atcactcttt ggtctatttg tcccatttga gtatacgatt ttggaatatc cattatttct
     4441 ttaaattcat caatactaac ggtatagtaa ccagttgacc gatattgcat taataagcga
     4501 aacaacgttt tagcataggt tgatcggata gcagtaaatg cagataactc gaacctacta
     4561 aattcagttg ttaagccatt aagaatacct tccaatttag gattgatact tacttcaact
     4621 gtttgttttt ttacattaac gtcaaagcca gtaaacaaga caaatcgtcg aaaacttccg
     4681 tcatcatcat actcattccg ataagttagt tgcagcattt tactgtatag gctatctaaa
     4741 tcatttgtaa acgccttaat tgcagtcatt ttatagtcgc ttaattcttt taactcttca
     4801 aaagaaaata tgatactgtc taaacctttg tctttcattc ttgcacataa ggcaaaaaat
     4861 aagttcattt ctttagcatt gaactgtttt aaagcaacta gatttaattc gttttgatat
     4921 ttaacagcta attccgattt ttcattcatt cctttcgccc cctcacgctc acattatata
     4981 aacagcatac attaatgtca agtatgctgt ttatataatg tgagcgtgat tttacaaaga
     5041 taatttgtta ttagtagtca gataaaatga tgttggtaca cagataaaat gatgttggta
     5101 cacagataaa atgatgttgg tacacagata aaatgatgtt ggtacacaga taaaatgatg
     5161 ttggtaccct ctgctcttcc ttaga
//
