LOCUS       NZ_CP116927             8412 bp    DNA     linear   CON 11-MAR-2024
DEFINITION  Escherichia coli strain MLI105 plasmid p8_MLI105, complete sequence.
ACCESSION   NZ_CP116927
VERSION     NZ_CP116927.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29553885
            Assembly: GCF_024700835.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Riediger,M. and Zautner,A.E.
  TITLE     Molecular epidemiology of Escherichia coli with resistance against
            third-generation cephalosporins isolated from stool samples of
            European soldiers with diarrhea on deployment in Western African
            Mali
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8412)
  AUTHORS   Riediger,M. and Zautner,A.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2023) Institute of Medical Microbiology and
            Hospital Hygiene, Medical Faculty, Otto-von-Guericke University
            Magdeburg, Leipziger Strasze 44, Magdeburg, Saxony-Anhalt 39120,
            Germany
REFERENCE   3  (bases 1 to 8412)
  AUTHORS   Zautner,A.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUL-2022) Institute of Medical Microbiology and
            Hospital Hygiene, Medical Faculty, Otto-von-Guericke University
            Magdeburg, Leipziger Strasze 44, Magdeburg, Saxony-Anhalt 39120,
            Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-JAN-2023
            Assembly Method :: trycycler v. 0.5.3; unicycler v. 0.5.0; skesa v.
            2.4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/11/2024 05:17:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,410
            Genes (coding)                    :: 4,206
            CDSs (with protein)               :: 4,206
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 204
            CDSs (without protein)            :: 204
            Pseudo Genes (ambiguous residues) :: 0 of 204
            Pseudo Genes (frameshifted)       :: 67 of 204
            Pseudo Genes (incomplete)         :: 140 of 204
            Pseudo Genes (internal stop)      :: 38 of 204
            Pseudo Genes (multiple problems)  :: 42 of 204
            Pseudo Genes (short protein)      :: 4 of 204
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116927.1.
            Bacteria and source DNA available from Institute of Medical
            Microbiology and Hospital Hygiene Medical Faculty Otto-von-Guericke
            University Magdeburg 39120 Magdeburg, Germany.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MLI105"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p8_MLI105"
                     /geo_loc_name="Mali: Koulikoro"
                     /lat_lon="12.52 N 7.34 W"
                     /collection_date="2014-06-24"
                     /collected_by="Bundeswehr Medical Service"
     gene            <1..97
                     /locus_tag="NL421_RS22610"
                     /pseudo=""
     CDS             <1..97
                     /locus_tag="NL421_RS22610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011161230.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=2
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /name="entry exclusion protein 1"
     gene            complement(875..1024)
                     /locus_tag="NL421_RS00070"
                     /old_locus_tag="NL421_000070"
     CDS             complement(875..1024)
                     /locus_tag="NL421_RS00070"
                     /old_locus_tag="NL421_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            1595..1753
                     /locus_tag="NL421_RS22615"
     CDS             1595..1753
                     /locus_tag="NL421_RS22615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            2213..3028
                     /gene="sul2"
                     /locus_tag="NL421_RS00075"
                     /old_locus_tag="NL421_000075"
     ARG             2214..3025
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3089..3892
                     /gene="aph(3'')-Ib"
                     /locus_tag="NL421_RS00080"
                     /old_locus_tag="NL421_000080"
     ARG             3090..3889
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3892..4728
                     /locus_tag="NL421_RS00085"
                     /old_locus_tag="NL421_000085"
     CDS             3892..4728
                     /locus_tag="NL421_RS00085"
                     /old_locus_tag="NL421_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            5034..5276
                     /locus_tag="NL421_RS00090"
                     /old_locus_tag="NL421_000090"
     CDS             5034..5276
                     /locus_tag="NL421_RS00090"
                     /old_locus_tag="NL421_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(5308..5958)
                     /gene="tetR(A)"
                     /locus_tag="NL421_RS00095"
                     /old_locus_tag="NL421_000095"
     CDS             complement(5308..5958)
                     /gene="tetR(A)"
                     /locus_tag="NL421_RS00095"
                     /old_locus_tag="NL421_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            6064..7263
                     /gene="tet(A)"
                     /locus_tag="NL421_RS00100"
                     /old_locus_tag="NL421_000100"
     ARG             6065..7260
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<7230..7418)
                     /locus_tag="NL421_RS00105"
                     /old_locus_tag="NL421_000105"
                     /pseudo=""
     CDS             complement(<7230..7418)
                     /locus_tag="NL421_RS00105"
                     /old_locus_tag="NL421_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(7462..7851)
                     /locus_tag="NL421_RS00110"
                     /old_locus_tag="NL421_000110"
     CDS             complement(7462..7851)
                     /locus_tag="NL421_RS00110"
                     /old_locus_tag="NL421_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            8069..>8412
                     /locus_tag="NL421_RS00115"
                     /old_locus_tag="NL421_000115"
     CDS             8069..>8412
                     /locus_tag="NL421_RS00115"
                     /old_locus_tag="NL421_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_272790413.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEER"
                     /name="entry exclusion protein 1"
     ARG             3893..4725
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(1060..1233)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          1157..1762
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.431"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 acaactacag gccgaaatta gccgtctggc tgacgcgctg gcacaggaaa aaaagcgggg
       61 attctggtcg cggctgtttc gctccggttc agagtaacag tgcaggtgac tctgatttac
      121 cataacatct gttatcagca ccgcctgaga ggccctcagg gcgtttctga cgggaaaccg
      181 gtaaaacagg caacaaacca cccgaaaacc cgccagaaac gcgcaggctc tgtttttacg
      241 gcatgcatga gtatgcgcac aagtgcatag cgcggaaatt cacaccattc agccggggta
      301 agtcctgttt ccggcgttct tcccatccga aaacggtgaa cggggacgca atttattgcg
      361 tggccgtgaa gcgttgccgg cgcagccgga aaacccgctg tcagcacgtt caggaagggg
      421 gtgatgtaca aatggcacag aggatgaaag tgacgtatga gctgaaggaa tgatgtaaaa
      481 gtgatgtata agtgatgtaa aagtgatgta aaagtgatgt ataagtgatg taaaagtgat
      541 gtatgaactg aaggagcgat gtaattctgt agtcaggaag aataacccgt tcatgcaggc
      601 atgaacgata cagtgaggaa cgcttttttt gtcacgatct ccccttaacc tgcgtaaatc
      661 acccgccatg gttgattatt tttcaacctc aaaatggtca tacacctaac gcagccgcga
      721 ttttcatcgc aactgcatga attaaaagaa aaaaaaggaa tatttcgttt tcacgagtaa
      781 tacccgggta ttaaaaatcc actgtaatta catgaaaata aagaaataaa aggtaatacc
      841 ggagtattac cacgtacgcg ggcaagcgaa aaagctataa cgcttcccgg ccgacaggcg
      901 acccaaaatc cacaggggca tgcaggttct cttcagtaac ctgcgccatt aaatcaaaaa
      961 ggtcatattc gccgggaagc tgcgtatcag tcgaatccag ttgttcatca tattcagatg
     1021 gcatggggtg atatccagta gctgagaggt tgttagcggg tttcggggcg cagccctgaa
     1081 ccagtcatgt agcgctagcg gagtgtatac tggcttacta tgttgccacg attgcgaaaa
     1141 tcagtgaagt gcttcatgtg gcaggagaaa aaaggcagca ccggcgcgtc agcagaatat
     1201 gcgatacagg agatattccg cttcctcgct cactgactcg ctacgctcgg tcgttcggct
     1261 gcggcgagcg gtaccggctt acggacgggg acggaaattt cctggaagat accaagaaga
     1321 cacttagcaa agaagagata aggccacggc gaagccgttt ttccataggc tccgcccccc
     1381 tgacaagcat cgcaaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata
     1441 aagatcccag gcgtttcccc ctggtagctc cctcgtgcgc tctcctgttc ctgcctttcg
     1501 gtttaccggt gtcattccgc tgttatggcc gcggtttctc attccacgcc tgacactcag
     1561 ttccgtgaag gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga
     1621 ccgctgcgcc ttgtccggta actatcaact tgagtccaac ccggaaagac acgacaaaac
     1681 gccactggca gcagccatta gtaacaggag tgcacgagaa gatagattaa gaggtcttga
     1741 agtagaggcc tgactgcggc tacactgaaa gaacagtttt ggtaatggcg gtctcgtgaa
     1801 ccctgttacc gggttaagca gttccccaac tgacttaacc ttcgaacaaa ccgcctcccc
     1861 aggcggtttt ttcgtttaca gagcaagaga ttacgattca ttgaaaagga tctcaagaag
     1921 atcctttgta gattactatc aattaatctt ccgaacatga caccagtcac tgttgattac
     1981 ggttaaaccg ctccgtaaca ggtttccctc gtattggtga cttgcagcac caaagaagca
     2041 aaaaagataa atatatctac ttattgagag atgatatatt tatttccaaa atgctacaga
     2101 tacatcatca tgaaacggat caccgcaaac aggttactcg cctggggatt ccctttcgac
     2161 ccgagcatcc gtatgagact catgctcgat tattattatt atagaagccc ccatgaataa
     2221 atcgctcatc attttcggca tcgtcaacat aacctcggac agtttctccg atggaggccg
     2281 gtatctggcg ccagacgcag ccattgcgca ggcgcgtaag ctgatggccg agggggcaga
     2341 tgtgatcgac ctcggtccgg catccagcaa tcccgacgcc gcgcctgttt cgtccgacac
     2401 agaaatcgcg cgtatcgcgc cggtgctgga cgcgctcaag gcagatggca ttcccgtctc
     2461 gctcgacagt tatcaacccg cgacgcaagc ctatgccttg tcgcgtggtg tggcctatct
     2521 caatgatatt cgcggttttc cagacgctgc gttctatccg caattggcga aatcatctgc
     2581 caaactcgtc gttatgcatt cggtgcaaga cgggcaggca gatcggcgcg aggcacccgc
     2641 tggcgacatc atggatcaca ttgcggcgtt ctttgacgcg cgcatcgcgg cgctgacggg
     2701 tgccggtatc aaacgcaacc gccttgtcct tgatcccggc atggggtttt ttctgggggc
     2761 tgctcccgaa acctcgctct cggtgctggc gcggttcgat gaattgcggc tgcgcttcga
     2821 tttgccggtg cttctgtctg tttcgcgcaa atcctttctg cgcgcgctca caggccgtgg
     2881 tccgggggat gtcggggccg cgacactcgc tgcagagctt gccgccgccg caggtggagc
     2941 tgacttcatc cgcacacacg agccgcgccc cttgcgcgac gggctggcgg tattggcggc
     3001 gctgaaagaa accgcaagaa ttcgttaact gcacattcgg gatatttctc tatattcgcg
     3061 cttcatcaga aaactgaagg aacctccatt gaatcgaact aatatttttt ttggtgaatc
     3121 gcattctgac tggttgcctg tcagaggcgg agaatctggt gattttgttt ttcgacgtgg
     3181 tgacgggcat gccttcgcga aaatcgcacc tgcttcccgc cgcggtgagc tcgctggaga
     3241 gcgtgaccgc ctcatttggc tcaaaggtcg aggtgtggct tgccccgagg tcatcaactg
     3301 gcaggaggaa caggagggtg catgcttggt gataacggca attccgggag taccggcggc
     3361 tgatctgtct ggagcggatt tgctcaaagc gtggccgtca atggggcagc aacttggcgc
     3421 tgttcacagc ctatcggttg atcaatgtcc gtttgagcgc aggctgtcgc gaatgttcgg
     3481 acgcgccgtt gatgtggtgt cccgcaatgc cgtcaatccc gacttcttac cggacgagga
     3541 caagagtacg ccgcagctcg atcttttggc tcgtgtcgaa cgagagctac cggtgcggct
     3601 cgaccaagag cgcaccgata tggttgtttg ccatggtgat ccctgcatgc cgaacttcat
     3661 ggtggaccct aaaactcttc aatgcacggg tctgatcgac cttgggcggc tcggaacagc
     3721 agatcgctat gccgatttgg cactcatgat tgctaacgcc gaagagaact gggcagcgcc
     3781 agatgaagca gagcgcgcct tcgctgtcct attcaatgta ttggggatcg aagcccccga
     3841 ccgcgaacgc cttgccttct atctgcgatt ggaccctctg acttggggtt gatgttcatg
     3901 ccgcctgttt ttcctgctca ttggcacgtt tcgcaacctg ttctcattgc ggacaccttt
     3961 tccagcctcg tttggaaagt ttcattgcca gacgggactc ctgcaatcgt caagggattg
     4021 aaacctatag aagacattgc tgatgaactg cgcggggccg actatctggt atggcgcaat
     4081 gggaggggag cagtccggtt gctcggtcgt gagaacaatc tgatgttgct cgaatatgcc
     4141 ggggagcgaa tgctctctca catcgttgcc gagcacggcg actaccaggc gaccgaaatt
     4201 gcagcggaac taatggcgaa gctgtatgcc gcatctgagg aacccctgcc ttctgccctt
     4261 ctcccgatcc gggatcgctt tgcagctttg tttcagcggg cgcgcgatga tcaaaacgca
     4321 ggttgtcaaa ctgactacgt ccacgcggcg attatagccg atcaaatgat gagcaatgcc
     4381 tcggaactgc gtgggctaca tggcgatctg catcatgaaa acatcatgtt ctccagtcgc
     4441 ggctggctgg tgatagatcc cgtcggtctg gtcggtgaag tgggctttgg cgccgccaat
     4501 atgttctacg atccggctga cagagacgac ctttgtctcg atcctagacg cattgcacag
     4561 atggcggacg cattctctcg tgcgctggac gtcgatccgc gtcgcctgct cgaccaggcg
     4621 tacgcttatg ggtgcctttc cgcagcttgg aacgcggatg gagaagagga gcaacgcgat
     4681 ctagctatcg cggccgcgat caagcaggtg cgacagacgt catactagat atcaagcgac
     4741 ttctcctatc ccctgggaac acatcaatct caccggagaa tatcgctggc caaagcctta
     4801 gcgtaggatt ccgccccttc ccgcaaacga cccctggaag ccccgtagcg acgcggagag
     4861 gggcgagaca agccaagggc gcaggctcga tgcgcagcac gacatagccg gttctcgcaa
     4921 ggacgagaat ttccctgcgg tgcccctcaa gtgtcaatga aagtttccaa cgcgagccat
     4981 tcgcgagagc cttgagtcca cgctagatct atctcatctg cgcaaggcag aacgtgaaga
     5041 cggccgccct ggacctcgcc cgcgagcgcc aggcgcacga ggccggcgcg cggacccgcg
     5101 ccacggccca cgagcggacg ccgcagcagg agcgccagaa ggccgccaga gaggccgagc
     5161 gcggccgtga ggcttggacg ctagggcagg gcatgaaaaa gcccgtagcg ggctgctacg
     5221 ggcgtctgac gcggtggaaa gggggagggg atgttgtcta catggctctg ctgtagtgag
     5281 tgggttgcgc tccggcagcg gtcctgatca atcgtcaccc tttctcggtc cttcaacgtt
     5341 cctgacaacg agcctccttt tcgccaatcc atcgacaatc accgcgagtc cctgctcgaa
     5401 cgctgcgtcc ggaccggctt cgtcgaaggc gtctatcgcg gcccgcaaca gcggcgagag
     5461 cggagcctgt tcaacggtgc cgccgcgctc gccggcatcg ctgtcgccgg cctgctcctc
     5521 aagcacggcc ccaacagtga agtagctgat tgtcatcagc gcattgacgg cgtccccggc
     5581 cgaaaaaccc gcctcgcaga ggaagcgaag ctgcgcgtcg gccgtttcca tctgcggtgc
     5641 gcccggtcgc gtgccggcat ggatgcgcgc gccatcgcgg taggcgagca gcgcctgcct
     5701 gaagctgcgg gcattcccga tcagaaatga gcgccagtcg tcgtcggctc tcggcaccga
     5761 atgcgtatga ttctccgcca gcatggcttc ggccagtgcg tcgagcagcg cccgcttgtt
     5821 cctgaagtgc cagtaaagcg ccggctgctg aacccccaac cgttccgcca gtttgcgtgt
     5881 cgtcagaccg tctacgccga cctcgttcaa caggtccagg gcggcacgga tcactgtatt
     5941 cggctgcaac tttgtcatgc ttgacacttt atcactgata aacataatat gtccaccaac
     6001 ttatcagtga taaagaatcc gcgcgttcaa tcggaccagc ggaggctggt ccggaggcca
     6061 gacgtgaaac ccaacagacc cctgatcgta attctgagca ctgtcgcgct cgacgctgtc
     6121 ggcatcggcc tgattatgcc ggtgctgccg ggcctcctgc gcgatctggt tcactcgaac
     6181 gacgtcaccg cccactatgg cattctgctg gcgctgtatg cgttgatgca atttgcctgc
     6241 gcacctgtgc tgggcgcgct gtcggatcgt ttcgggcggc ggccggtctt gctcgtctcg
     6301 ctggccggcg ctgctgtcga ctacgccatc atggcgacgg cgcctttcct ttgggttctc
     6361 tatatcgggc ggatcgtggc cggcatcacc ggggcgactg gggcggtagc cggcgcttat
     6421 attgccgata tcactgatgg cgatgagcgc gcgcggcact tcggcttcat gagcgcctgt
     6481 ttcgggttcg ggatggtcgc gggacctgtg ctcggtgggc tgatgggcgg tttctccccc
     6541 cacgctccgt tcttcgccgc ggcagccttg aacggcctca atttcctgac gggctgtttc
     6601 cttttgccgg agtcgcacaa aggcgaacgc cggccgttac gccgggaggc tctcaacccg
     6661 ctcgcttcgt tccggtgggc ccggggcatg accgtcgtcg ccgccctgat ggcggtcttc
     6721 ttcatcatgc aacttgtcgg acaggtgccg gccgcgcttt gggtcatttt cggcgaggat
     6781 cgctttcact gggacgcgac cacgatcggc atttcgcttg ccgcatttgg cattctgcat
     6841 tcactcgccc aggcaatgat caccggccct gtagccgccc ggctcggcga aaggcgggca
     6901 ctcatgctcg gaatgattgc cgacggcaca ggctacatcc tgcttgcctt cgcgacacgg
     6961 ggatggatgg cgttcccgat catggtcctg cttgcttcgg gtggcatcgg aatgccggcg
     7021 ctgcaagcaa tgttgtccag gcaggtggat gaggaacgtc aggggcagct gcaaggctca
     7081 ctggcggcgc tcaccagcct gacctcgatc gtcggacccc tcctcttcac ggcgatctat
     7141 gcggcttcta taacaacgtg gaacgggtgg gcatggattg caggcgctgc cctctacttg
     7201 ctctgcctgc cggcgctgcg tcgcgggctt tggagaaatt cttcaaattc ccgttgcaca
     7261 tagcccggca attcctttcc ctgctctgcc ataagcgcag cgaatgccgg gtaatactcg
     7321 tcaacgatct gatagagaag ggtttgctcg ggtcggtggc tctggtaacg accagtatcc
     7381 cgatcccggc tggccgtcct ggccgccaca tgaggcatgt tccgcgtcct tgcaatactg
     7441 tgtttacata cagtctatcg cttagcggaa agttctttta ccctcagccg aaatgcctgc
     7501 cgttgctaga cattgccagc cagtgcccgt cactcccact cttacattgt tcgcagtatc
     7561 taggtacatg ttattgcgtg caccttcatg atgtataatg ttattaatga cagcagaaag
     7621 atcgacagaa ttaatattct tcgtacctgg gacagttatc gggtgtcggc cagtgatgag
     7681 accaaaggat gcagtataag atgaaccact actattatca tggccaccat taccaccacc
     7741 gttaaaacta cctcccgaac ttccagagcc gctatgacca tcattgtagg ggtcatttgg
     7801 tcccatgtga ccaaaaccac cgacagtacc gctatttaac tctgtactca taaaattatc
     7861 ctctttgact tttaaaaaca gcaaaaactg tatttataac aatgtgttta tatacagtat
     7921 tcgaaatact acgcccgtca aaaccacacc gtcaacaggt tttttgtgta tttttcacgc
     7981 ctcacgggca gcctcagacc ttttgtgtta aaacagtaat atgttctgct gttacaatac
     8041 ggtacacaca ggagacactg tcacttttat ggcatggcac acagttcaag agacactgaa
     8101 attaacagga aagtcgcgct cgcaattata tcgtgacatg gctaaaggcc ttgtcagcta
     8161 tcgcacagga aaaaatgatc gccgggagtt cgagaccagt gaactgattc gggcctatgg
     8221 cgagctgcgc acttctgaga caccaaaaag gcacaaggag ggacactcca aatctgtcgt
     8281 tagccaggcg gaaaatgctc agctagacgt cattcagcag aaactggacg ctctgcagca
     8341 gactgttacc ctaatgcttg aagacaagat ggcgcgggaa gcagaaaacc gcctgcgaaa
     8401 tgaggagagg ga
//
