LOCUS       NZ_CP116926            11848 bp    DNA     linear   CON 11-MAR-2024
DEFINITION  Escherichia coli strain MLI105 plasmid p11_MLI105, complete
            sequence.
ACCESSION   NZ_CP116926
VERSION     NZ_CP116926.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN29553885
            Assembly: GCF_024700835.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11848)
  AUTHORS   Riediger,M. and Zautner,A.E.
  TITLE     Molecular epidemiology of Escherichia coli with resistance against
            third-generation cephalosporins isolated from stool samples of
            European soldiers with diarrhea on deployment in Western African
            Mali
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11848)
  AUTHORS   Riediger,M. and Zautner,A.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2023) Institute of Medical Microbiology and
            Hospital Hygiene, Medical Faculty, Otto-von-Guericke University
            Magdeburg, Leipziger Strasze 44, Magdeburg, Saxony-Anhalt 39120,
            Germany
REFERENCE   3  (bases 1 to 11848)
  AUTHORS   Zautner,A.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JUL-2022) Institute of Medical Microbiology and
            Hospital Hygiene, Medical Faculty, Otto-von-Guericke University
            Magdeburg, Leipziger Strasze 44, Magdeburg, Saxony-Anhalt 39120,
            Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 25-JAN-2023
            Assembly Method :: trycycler v. 0.5.3; unicycler v. 0.5.0; skesa v.
            2.4.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/11/2024 05:17:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,532
            CDSs (total)                      :: 4,410
            Genes (coding)                    :: 4,206
            CDSs (with protein)               :: 4,206
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 204
            CDSs (without protein)            :: 204
            Pseudo Genes (ambiguous residues) :: 0 of 204
            Pseudo Genes (frameshifted)       :: 67 of 204
            Pseudo Genes (incomplete)         :: 140 of 204
            Pseudo Genes (internal stop)      :: 38 of 204
            Pseudo Genes (multiple problems)  :: 42 of 204
            Pseudo Genes (short protein)      :: 4 of 204
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116926.1.
            Bacteria and source DNA available from Institute of Medical
            Microbiology and Hospital Hygiene Medical Faculty Otto-von-Guericke
            University Magdeburg 39120 Magdeburg, Germany.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="MLI105"
                     /isolation_source="stool sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p11_MLI105"
                     /geo_loc_name="Mali: Koulikoro"
                     /lat_lon="12.52 N 7.34 W"
                     /collection_date="2014-06-24"
                     /collected_by="Bundeswehr Medical Service"
     gene            70..975
                     /locus_tag="NL421_RS00005"
                     /old_locus_tag="NL421_000005"
     CDS             70..975
                     /locus_tag="NL421_RS00005"
                     /old_locus_tag="NL421_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="WP_000219391.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVDD
                     GRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMV
                     IQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDV
                     DRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSE
                     ARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFA
                     LDSGNEEYLAAAKAQLAAAE"
                     /name="Mph(A) family macrolide 2'-phosphotransferase"
     gene            972..2210
                     /gene="mrx(A)"
                     /locus_tag="NL421_RS00010"
                     /old_locus_tag="NL421_000010"
                     /gene_synonym="mrx"
     CDS             972..2210
                     /gene="mrx(A)"
                     /locus_tag="NL421_RS00010"
                     /old_locus_tag="NL421_000010"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="WP_000004159.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWAG
                     ATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTGLI
                     ACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGAAIG
                     LLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLLRPLF
                     GIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVGARISA
                     QNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRTPPELLG
                     RVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQFATMAAA
                     ATASAPTHTEGEH"
                     /name="mrx(A)"
     gene            2210..2794
                     /gene="mphR(A)"
                     /locus_tag="NL421_RS00015"
                     /old_locus_tag="NL421_000015"
     CDS             2210..2794
                     /gene="mphR(A)"
                     /locus_tag="NL421_RS00015"
                     /old_locus_tag="NL421_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="WP_001137892.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAALI
                     QRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVNYL
                     ISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVDPDG
                     ELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
                     /name="mphR(A)"
     gene            complement(3287..4051)
                     /locus_tag="NL421_RS00020"
                     /old_locus_tag="NL421_000020"
     CDS             complement(3287..4051)
                     /locus_tag="NL421_RS00020"
                     /old_locus_tag="NL421_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="WP_001389365.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDHT
                     TIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTIDFY
                     LSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETAHRQ
                     VKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQPGIR
                     GEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
                     /name="IS6-like element IS6100 family transposase"
     gene            4353..4910
                     /locus_tag="NL421_RS00025"
                     /old_locus_tag="NL421_000025"
     CDS             4353..4910
                     /locus_tag="NL421_RS00025"
                     /old_locus_tag="NL421_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001235713.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            5093..5953
                     /locus_tag="NL421_RS00030"
                     /old_locus_tag="NL421_000030"
     CDS             5093..5953
                     /locus_tag="NL421_RS00030"
                     /old_locus_tag="NL421_000030"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            complement(6337..6660)
                     /gene="mobA"
                     /locus_tag="NL421_RS00035"
                     /old_locus_tag="NL421_000035"
     CDS             complement(6337..6660)
                     /gene="mobA"
                     /locus_tag="NL421_RS00035"
                     /old_locus_tag="NL421_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            6944..7138
                     /locus_tag="NL421_RS00040"
                     /old_locus_tag="NL421_000040"
     CDS             6944..7138
                     /locus_tag="NL421_RS00040"
                     /old_locus_tag="NL421_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001485273.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEAENLTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(7976..8179)
                     /locus_tag="NL421_RS00045"
                     /old_locus_tag="NL421_000045"
     CDS             complement(7976..8179)
                     /locus_tag="NL421_RS00045"
                     /old_locus_tag="NL421_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="retron Se72 family effector protein"
                     /protein_id="WP_000427298.1"
                     /translation="MENGFVNFYDHVKGYGFIRRERGRDVFFRYDDFLFLGHDVDICKG
                     ILVRFKLEKTDKGFKAVAIQKV"
                     /name="retron Se72 family effector protein"
     gene            complement(8184..9323)
                     /locus_tag="NL421_RS00050"
                     /old_locus_tag="NL421_000050"
     CDS             complement(8184..9323)
                     /locus_tag="NL421_RS00050"
                     /old_locus_tag="NL421_000050"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900598.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="WP_005761319.1"
                     /translation="MNKPRFNGTPVASLDSLSAMLGIERKRLDWIVKSVSMSYKQFKVE
                     TGKNKKERQIFEPKRSLKGIQKKINKEIFEKIDYPHYLHGALSGRDYISNAAVHTRKRT
                     VICLDITNFYPSISKKDVCSIFKNLMRFSPDVSLCLTELVTLNNKVPQGGCCSSYIANL
                     LFFNSEYNLYNRLKSMGLSYSRLLDDITISSDKDLSSEEKTKVIKLVHGMVNQYRLSIN
                     ESKTTIEHSKDSSSKLSVTGLWVKHGVPKLTKENRRYIRYLVYICKKQGAYERHTKEYH
                     DLWNRCSGKVAQMSRLGHVQAVELRAILSEIMPVYDDYKISKLKLMAKHYLNKFTPPLT
                     DDQIRKIDRMLYDFDIVGRTNKNLAKLYRRKLVALLPDR"
                     /name="reverse transcriptase family protein"
     gene            complement(9897..10187)
                     /locus_tag="NL421_RS00055"
                     /old_locus_tag="NL421_000055"
     CDS             complement(9897..10187)
                     /locus_tag="NL421_RS00055"
                     /old_locus_tag="NL421_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032280127.1"
                     /translation="MNISIKNNDLKTVARFDGKTLRDAFSRKIATFDGKVLRDASLKKV
                     ATFDGKVLRDASLKKVATFDGQNLRDASSKIILSADAEVGIVVMAFVAELI"
                     /name="hypothetical protein"
     gene            complement(<10272..>11027)
                     /locus_tag="NL421_RS00060"
                     /old_locus_tag="NL421_000060"
                     /pseudo=""
     CDS             complement(<10272..>11027)
                     /locus_tag="NL421_RS00060"
                     /old_locus_tag="NL421_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            11092..11796
                     /locus_tag="NL421_RS00065"
                     /old_locus_tag="NL421_000065"
     CDS             11092..11796
                     /locus_tag="NL421_RS00065"
                     /old_locus_tag="NL421_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     ARG             71..972
                     /name="mph(A)"
                     /gene="mph(A)"
                     /gene_name="Mph(A) family macrolide 2'-phosphotransferase"
                     /tool="amrfinderplus"
                     /drug_class="MACROLIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             5094..5950
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             71..975
                     /name="mphA"
                     /gene="mphA"
                     /gene_name="macrolide phosphotransferase (MPH)"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             973..2210
                     /name="Mrx"
                     /gene="Mrx"
                     /gene_name="macrolide phosphotransferase (MPH)"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(6670..6909)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.755"
                     /coverage="100.0"
                     /evalue="7.66e-118"
     Typing          7174..7695
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.476"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          complement(6341..6660)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="3.15e-62"
ORIGIN
        1 ggattgaata taaccgacgt gactgttaca tttaggtggc taaacccgtc aagccctcag
       61 gagtgaatca tgaccgtagt cacgaccgcc gatacctccc aactgtacgc acttgcagcc
      121 cgacatgggc tcaagctcca tggcccgctg actgtcaatg agcttgggct cgactatagg
      181 atcgtgatcg ccaccgtcga cgatggacgt cggtgggtgc tgcgcatccc gcgccgagcc
      241 gaggtaagcg cgaaggtcga accagaggcg cgggtgctgg caatgctcaa gaatcgcctg
      301 ccgttcgcgg tgccggactg gcgcgtggcc aacgccgagc tcgttgccta tcccatgctc
      361 gaagactcga ctgcgatggt catccagcct ggttcgtcca cgcccgactg ggtcgtgccg
      421 caggactcgg aggtcttcgc ggagagcttc gcgaccgcgc tcgccgccct gcatgccgtc
      481 cccatttccg ccgccgtgga tgcggggatg ctcatccgta caccgacgca ggcccgtcag
      541 aaggtggccg acgacgttga ccgcgtccga cgcgagttcg tggtgaacga caagcgcctc
      601 caccggtggc agcgctggct cgacgacgat tcgtcgtggc cagatttctc cgtggtggtg
      661 catggcgatc tctacgtggg ccatgtgctc atcgacaaca cggagcgcgt cagcgggatg
      721 atcgactgga gcgaggcccg cgttgatgac cctgccatcg acatggccgc gcaccttatg
      781 gtctttggtg aagaggggct cgcgaagctc ctcctcacgt atgaagcggc cggtggccgg
      841 gtgtggccgc ggctcgccca ccacatcgcg gagcgccttg cgttcggggc ggtcacctac
      901 gcactcttcg ccctcgactc gggtaacgaa gagtacctcg ctgcggcgaa ggcgcagctc
      961 gccgcagcgg aatgagcgaa cgtcgatata gcccgctcgc gacgctgttc gcggcgacct
     1021 ttctcttccg gatcggcaac gcggtggcgg ccctcgcgct tccatggttc gtcctgtctc
     1081 atacaaagag cgcggcctgg gcgggcgcca cggccgctag cagcgtcatc gcgaccatca
     1141 tcggcgcgtg ggttggtggt ggcctcgtcg atcggttcgg gcgcgcgccc gtcgcattga
     1201 tctcgggtgt ggtgggcggc gtggccatgg cgagcatccc actgctcgat gccgttggcg
     1261 ccctctcgaa cactgggctg atcgcttgcg tggtgctcgg tgccgcgttc gacgcacccg
     1321 gtatggccgc gcaggacagt gagctgccca aactcggcca cgtcgccggg ctctccgttg
     1381 agcgcgtctc gtcactgaaa gcggtgatcg ggaacgtcgc gattctaggt ggcccggccc
     1441 ttgggggggc cgcaatcggc ctgcttggcg ctgcgccaac gctcgggctg acggcgttct
     1501 gctccgtcct tgcaggtctg ctcggcgcgt gggtgcttcc cgcgcgtgcc gctcggacga
     1561 tgaccacgac ggcgactctc tccatgcgcg ccggcgtcgc ttttctctgg agcgaacccc
     1621 tgctgcgccc tctctttggt atagtgatga tcttcgtggg catcgttggc gccaacggca
     1681 gcgtcatcat gcctgcgctg tttgtagatg caggacgcca agtagcagag ctcgggctgt
     1741 tctcctcaat gatgggggct ggtggtctcc ttggcattgc cattcatgcg tcggtcggcg
     1801 cccggatatc agcgcagaac tggctggcgg tggcattttg tggctctgcg gtgggctcgc
     1861 ttctgctttc acagttgcca ggcgtgccgg tgctgatgtt gttgggcgcg ctcgtgggac
     1921 tgctgaccgg ctcagtctct cccattctca acgctgccat ctacaaccgc acgccgccag
     1981 aacttctcgg ccgggtactc ggcacggtct cggcggtgat gctgtcagcc tcgcccatgg
     2041 ttatgcttgc ggccggcgcg tttgtcgacc ttgctggtcc gctccctggc ctcgttgtat
     2101 cggccgtgtt tgcggggctc gtggctctac tctcgctccg tcttcaattt gctacaatgg
     2161 cggcggcagc cacagcctcc gccccaaccc atacagaagg tgaacactga tgccccgccc
     2221 caagctcaag tccgatgacg aggtactcga ggccgccacc gtagtgctga agcgttgcgg
     2281 tcccatagag ttcacgctca gcggagtagc aaaggaggtg gggctctccc gcgcagcgtt
     2341 aatccagcgc ttcaccaacc gcgatacgct gctggtgagg atgatggagc gcggcgtcga
     2401 gcaggtgcgg cattacctga atgcgatacc gataggcgca gggccgcaag ggctctggga
     2461 atttttgcag gtgctcgttc ggagcatgaa cactcgcaac gacttctcgg tgaactatct
     2521 catctcctgg tacgagctcc aggtgccgga gctacgcacg cttgcgatcc agcggaaccg
     2581 cgcggtggtg gaggggatcc gcaagcgact gcccccaggt gctcctgcgg cagctgagtt
     2641 gctcctgcac tcggtcatcg ctggcgcgac gatgcagtgg gccgtcgatc cggatggtga
     2701 gctagctgat catgtgctgg ctcagatcgc tgccatcctg tgtttaatgt ttcccgaaca
     2761 cgacgatttc caactcctcc aggcacatgc gtaaacggag gtgtgcagag tccctgcggc
     2821 aggcgacgaa cacgaccgtc gtcgattagt accggtacgg tcggtggtat cgaagtcttg
     2881 atcaccactc aggtctacgg cttacaaatg gtgaccatcc cgatacttgc gtcagagcac
     2941 cgggccgatt ctttgacagt gaatcactcc cgtaaggttg tgccggtgtg ggtgtcccgg
     3001 gtcgagacga tactccgcca atgcgcccag caaacaacct ggccatcgca ggtggtgggg
     3061 agcggtgtgg cggatgagtt ggacaagttg gtgtagcagc acgagcacgg cgagataaca
     3121 tcgcaggagt tcgacatgct caagagacag ctgattgcga atcgcgatgc agattcataa
     3181 cccgattgcg ggttggcttc actccaccat caccgagcag actagcacgg cgggctctgt
     3241 tgcaaagatt ggcggcagtc agaggtaggc tgtcgctctg cgccgatcag gcggctgctg
     3301 cgaaatggtg gttgagcatg cccatggcct ccgtcagcgc cgagggccca atgccaaaag
     3361 ctctctccac aaggcgcacc tcgcccctga tgccgggctg caggcaccag gggcgagcct
     3421 gtcctttgcg cagggctcgc atgacttcga atcccttgat cgtggcatag gccgtgggga
     3481 tcgatttgaa accgcgcacc ggcttgatca gtatcttgag ctttccgtga tcggcctcga
     3541 tcacgttatt gagatacttc acctgccggt gggccgtctc ccggtccagc tttccttcgc
     3601 gcttcaattc ggtgatcgct gcaccatagc tcggcgcttt gtcggtattg agcgtggcag
     3661 gcttttccca gtgcttcagg cctcgcaggg ccttgcccag gaaccgcttc gctgccttgg
     3721 cgctgcgggt cggcgacagg tagaaatcga tcgtgtcgcc ccgcttgtcg actgcccggt
     3781 acaggtaggt ccacttgccc cgcaccttga cgtaggtttc atccaggcgc cagctcggat
     3841 caaagccacg ccgccagaac cagcgcagcc gcttctccat ctccggggcg tagcactgga
     3901 cccagcgata gatcgtcgta tggtcgaccg aaatgccgcg ttccgccagc atttcctcaa
     3961 ggtcgcgata gctgatcgga tagcgacaat accagcgcac cgcccacagg atcacatcac
     4021 cctggaaatg gcgccacttg aaatccgtca tcgttccgtc cgtccaatct ccgccaagca
     4081 tgctcaagct tcacgatttt tgcaacagag ccagctgaag ctcatccggt tcaccggtga
     4141 atctgccata gctttcagtc tgctcagtgg tcagaaagtc aacgggcata tcggcctccc
     4201 tgcctgacgg gcatttagta acatttttcc aaccgtacga aatgttataa attatcggac
     4261 atcgtaaaac tgttacatta atatgtctat taaatcgtaa atttgtaata atagacatga
     4321 gttgtccgat attcgattta aggtacattt ttatgcgact ttttggttac gctcgggtct
     4381 caaccagtca gcagtctctt gatcttcagg tcagagcact caaagacgca ggtgtgaaag
     4441 caaaccgtat atttaccgat aaggcatccg gcagttcaac agaccgggaa gggctggatt
     4501 tgctgaggat gaaggtggag gaaggtgatg tcattctggt taagaagctc gaccgtcttg
     4561 gccgcgacac tgccgatatg atccaactga taaaggaatt tgacgctcag ggcgtggcag
     4621 tccggttcat tgatgacggg atcagtaccg acggtgatat ggggcaaatg gtggtcacca
     4681 tcctgtcggc tgtggcacag gctgaacgcc ggaggatcct agaacgcacg aatgagggcc
     4741 gacaggaagc aaagctgaaa ggaatcaaat ttggccgcag gcgtaccgtg gacaggaacg
     4801 tcgtgctgac gcttcatcag aagggcactg gtgcaacgga aattgctcat cagctcagta
     4861 ttgcccgctc cacggtttat aaaattcttg aagacgaaag ggcctcgtga tacgcctatt
     4921 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     4981 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     5041 catgagacaa taaccctggt aaatgcttca ataatattga aaaaggaaga gtatgagtat
     5101 tcaacatttt cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     5161 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     5221 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     5281 ttttccaatg atgagcactt ttaaagttct gctatgtggt gcggtattat cccgtgttga
     5341 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     5401 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     5461 tgccataacc atgagtgata acactgctgc caacttactt ctgacaacga tcggaggacc
     5521 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     5581 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc
     5641 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5701 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5761 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5821 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5881 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5941 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     6001 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     6061 cccttaacgt gagttttcgt tccactgagc gtcagacccc taatttaacc aattgtattt
     6121 accataatcc cttaattgta cgcaccacag aatcgcgctg agcgcgattc tgacacagac
     6181 actgcaaaaa gagatgtttt accctaagaa aaccggggaa atgcgtcagg atcgttttca
     6241 gctcgctgca tagctatgca tgaaagcgag tggtgatcac tttgggagct tacagtgttc
     6301 ataccgtctg ttttcgacag ttttctcccc tggaagctaa tctgccataa gcctggataa
     6361 cagggcacgg tgatactccg taatagcgat cagcacctcc gcatactccc tgtccccgac
     6421 acgcttgccg tcgataaaga gttttttctg taacgccccc agtctcataa cttctttcag
     6481 tacccgatca tcagtcagtg agttaacttt cttaccgaga gctgccgccc gtaaaaaacc
     6541 ggaaacggtc ttgccgcatt cagcagccct ttttctgatt tcctggtctt cttctgccgt
     6601 cagacgaaca gggcgactga tagcccgcct gcgcgtatta cttccacttc gttttgtcat
     6661 aaaacatggc tccgtatctg acatgggtgt cggggcaaag ccctgaccag ggcaattgta
     6721 atagcgtgca tgtatgcgcg gtataacaat tgcacatcct gtcctgtcag caagctcgaa
     6781 ttaccgacaa aggcacggca gttttttggc agtgacagtg ttgtctgaca gaaatgcccg
     6841 tcaaagagtc agacagcaga ataccgacgg taaaacagtc gaaaagtggt acgcgatata
     6901 atcacacaac gcagatgaca gaaagaacgg agaaaacaga aagatgaaca agcagcagca
     6961 aaccgtactc aacatggcag gattcataaa aagccagagc ctgacgctgc tcgaaaaact
     7021 ggacgcactt gatgctgacg aacaggccgc catgtgtgag aagctgcacg aactcgcgga
     7081 agaacttcag aacagcatac agacacgctt tgaagcggaa aatcttaccg ggatataacg
     7141 catccggaca ggatgcagaa cggcatagag gagcgtaagg agaaaatgtc acataaggcg
     7201 ctcttccgct tcctcgctca ctgactcgct acgctcggtc gttcgactgc ggcgagcggt
     7261 gtcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgccggaaa
     7321 gaacatgtga gcaaaaagcc gaagccaggg acaaaggccg cgccgatggc gtttttccat
     7381 aggctccgcc cccctgacga gcatcacaaa aaccgacgct caagtcagag gtggcgaaac
     7441 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7501 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aaacgtggcg
     7561 ctttctcata gctcacgctg ttggtatctc ggcccggtgt aggtcgttcg ctccaagctg
     7621 gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcga
     7681 cttgaatcca aaccgagaag acacgactta tcgccactgg cagcagccac tggtaaccga
     7741 attagaggag ttagccttga agtcatgcgc ctgttaaggc taaactgaaa gaacaagttt
     7801 tggtgactgc gctcctccaa gccagttacc tcggctcaaa gagttggtag ctctgagaac
     7861 cttcgaaaaa ccgccctgca aggcggtttt ttcgtttaca cagcaagaga ttacgacgag
     7921 atagaaagga tctcaagttc tcatatcata aaaacaagct gacacatcca cgatgttata
     7981 ccttttgaat cgctacagct ttaaaccctt tatctgtttt ttctaattta aacctaacta
     8041 aaataccttt acatatatcg acatcatgcc ctaaaaacaa aaaatcatca taccgaaaga
     8101 aaacatcacg tccacgctca cgccttataa agccataccc tttaacatgg tcataaaaat
     8161 taacaaatcc attttccata taatcaccta tctggaagta atgccacgag cttacgtcta
     8221 tatagttttg caagattctt attagtccgc cctactatat caaaatcata gagcatccta
     8281 tctattttcc taatctgatc atcagttaaa ggcggcgtaa acttatttag atagtgctta
     8341 gccatcaact ttaatttaga tatcttgtaa tcatcataaa ctggcattat ctcagaaaga
     8401 atcgccctca attcaaccgc ctggacatgc ccaagcctcg acatttgagc aaccttgcca
     8461 gagcatctat tccataaatc atggtactct ttcgtatgac gttcataagc accttgtttt
     8521 ttacatatat aaactaagta cctgatataa cgtctatttt cttttgttaa cttaggaaca
     8581 ccatgcttta cccacaaccc agtgacacta agtttagaag acgaatcttt tgagtgctct
     8641 atcgttgtct tactttcatt tattgaaagt ctgtactgat tcaccattcc gtgaacgagt
     8701 tttattactt ttgttttttc ctctgatgaa agatccttat cagaggatat agtaatatca
     8761 tctaaaagcc ttgaatatga aagtcccata gacttcaatc tattatagag attatactca
     8821 ctattaaaaa ataaaagatt tgcaatataa ctagaacagc acccaccctg agggacctta
     8881 ttattcaacg tcacaagttc cgttaagcat aaagaaacat caggagaaaa acgcattaaa
     8941 tttttaaaaa tagaacaaac atctttttta ctaatgctgg gataaaaatt ggtgatatca
     9001 agacatataa ctgttctttt cctagtatga acagcggcat tagatatata atcgcgacca
     9061 cttaacgccc catgcaaata atgagggtaa tcaattttct caaaaatctc tttatttatt
     9121 tttttttgaa ttcctttcaa agatctcttg ggttcaaaaa tttgcctttc ttttttattc
     9181 ttccctgttt caaccttaaa ctgcttgtac gacatcgata cagacttgac tatccagtcc
     9241 aatctttttc tttctatgcc taacatcgca gataaagaat ccaaagatgc aacaggagtt
     9301 ccattaaacc tgggtttatt catgatgttt gagttaaaag tcgattagtt aaaaagtttc
     9361 gatacgtaat ctattacttt aaacataatc aagcacatag aaaacaaaaa ctgtgttttt
     9421 ttcagtgcct ccagagagcc aacctctctt tttttcttca tctgaccgtc tccagaacaa
     9481 gatgaagagc tcttggttac gttaatgttg atgttgataa taaacatgat gcaactccta
     9541 ttggttatga aattttgcca gaccagtagg aaagcaagga aaaactctat tggctctttg
     9601 ggacttttaa ctcacattgc cagacaatcg tcgctattct tttagtgctg atacacgctc
     9661 aggggcgttc tcaccattaa ttagcggcca ggccggtcat gatctcatgc gagatcgcct
     9721 taaaaacaag gcatcggttt ccttgcgcaa catacacgta accatttgtt aaagagcgag
     9781 agccaggcgt ggttcgcccg cttcgctgat tattataaag ttttttatct ttctgtcacc
     9841 aacaagaaag cgccttatcc tgccagcgat aggctaaaag gtatgcagca tgagattcaa
     9901 atcaactcag ctacaaaggc cataacaaca atcccaacct cagcatcggc actcaggata
     9961 atttttgaac tagcatctct taaattctga ccgtcaaagg ttgctacttt ttttaaagag
    10021 gcatccctta aaaccttgcc atcaaaagtc gccacttttt ttaaagaggc atcccttaaa
    10081 accttgccat caaaagtcgc tatttttctc gaaaaggcat ctcttaaggt tttaccgtca
    10141 aaccgagcaa cggtttttaa gtcattgttt tttattgaaa tattcatttc tcaccaatag
    10201 taattggggt ctgacgctca gtggaacgaa aactcacgtt aagcaacgtt ttctgcctct
    10261 gacgcctctt ttaatggtct cagatgtcct ttggtcacca gttctgccag cgtgaaggaa
    10321 taatggccga gcatattgat atgtccgtgg caaagcgggg agaggcgtgc gatatcttca
    10381 tcattcagtg tttcaccctg cgcccggaga tgatccaggg ctgcctgcat ataaatagtg
    10441 ttccataaca cgacggcgtt agtgaccagc cccagtgtgc ccagttgatc ttcctgaccg
    10501 tcggtatatc gttttcttat ctcacctttt tgaccgtgac agatggctct ggcaacggca
    10561 tggcgacttt ctccccgatt aagctgggtc agaatgcgcc ggcggtaatc ttcatcatca
    10621 atataattaa gcagatacag cgttttgttg atgcgcccca cttcaatgat tgcctgagtc
    10681 agtccggaag gacgttcact tttcagcaat gaacggacca gcactgaaac ctgtactttg
    10741 cccagcttca gggagccagc ggtccggatc atttcgtccc actgaaggac tatttttcgg
    10801 ggatctgatt gccctctggc aatatcattc agcacgccat agtcggcatc atggtccatt
    10861 cgccagaaaa ccgaagcacc ggcatcagcc aggcgtggag aaaactggta tcccagcagc
    10921 cagaaaaggc caaagacaag ttcgctggca cctgctgtat cggtcataat ttcggttgga
    10981 ttcagcccgg tctcctgttc cagaagacct tccagcacaa agatagaggg cactgttgca
    11041 aatagtcggt ggtgataaac ttatcatccc cttttgctga tggagctgca catgaaccca
    11101 ttcaaaggcc ggcattttca gcgtgacatc attctgtggg ccgtacgctg gtactgcaaa
    11161 tacggcatca gttaccgtga gctgcaggag atgctggctg aacgcggagt gaatgtcgat
    11221 cactccacga tttaccgctg ggttcagcgt tatgcgcctg aaatggaaaa acggctgcgc
    11281 tggtactggc gtaacccttc cgatctttgc ccgtggcaca tggatgaaac ctacgtgaag
    11341 gtcaatggcc gctgggcgta tctgtaccgg gccgtcgaca gccggggccg cactgtcgat
    11401 ttttatctct cctcccgtcg taacagcaaa gctgcatacc ggtttctggg taaaatcctc
    11461 aacaacgtga agaagtggca gatcccgcga ttcatcaaca cggataaagc gcccgcctat
    11521 ggtcgcgcgc ttgctctgct caaacgcgaa ggccggtgcc cgtctgacgt tgaacaccga
    11581 cagattaagt accggaacaa cgtgattgaa tgcgatcatg gcaaactgaa acggataatc
    11641 ggcgccacgc tgggatttaa atccatgaag acggcttacg ccaccatcaa aggtattgag
    11701 gtgatgcgtg cactacgcaa aggccaggcc tcagcatttt attatggtga tcccctgggc
    11761 gaaatgcgcc tggtaagcag agtttttgaa atgtaaggcc tttgaataag acaaaaggct
    11821 gcctcatcgc taactttgca acagtgcc
//
