LOCUS       NZ_CP116908            11192 bp    DNA     circular CON 10-MAR-2024
DEFINITION  Klebsiella pneumoniae strain KP2722 plasmid pKP2722-5, complete
            sequence.
ACCESSION   NZ_CP116908
VERSION     NZ_CP116908.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32946429
            Assembly: GCF_028471925.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11192)
  AUTHORS   Li,X., Cui,X., Wang,W. and Wei,J.
  TITLE     Whole-genome analysis of Klebsiella pneumoniae strain KP2722
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11192)
  AUTHORS   Li,X., Cui,X., Wang,W. and Wei,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2023) Zhuhai hospital, Jinan University, 79
            Kangning Road, Zhuhai, Guangdong 519000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Hifiasm v. 0.13-r308; Canu v. 0.7; Pilon v. 1.22
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.88x
            Sequencing Technology             :: Illumina NovaSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/09/2024 04:00:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,125
            CDSs (total)                      :: 6,003
            Genes (coding)                    :: 5,825
            CDSs (with protein)               :: 5,825
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 178
            CDSs (without protein)            :: 178
            Pseudo Genes (ambiguous residues) :: 0 of 178
            Pseudo Genes (frameshifted)       :: 64 of 178
            Pseudo Genes (incomplete)         :: 124 of 178
            Pseudo Genes (internal stop)      :: 21 of 178
            Pseudo Genes (multiple problems)  :: 31 of 178
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116908.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11192
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP2722"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP2722-5"
                     /geo_loc_name="China:Zhuhai"
                     /lat_lon="22.28 N 113.58 E"
                     /collection_date="2022"
                     /collected_by="Wei Jie"
     gene            complement(join(11135..11192,1..269))
                     /locus_tag="PO783_RS30435"
                     /old_locus_tag="PO783_30435"
     CDS             complement(join(11135..11192,1..269))
                     /locus_tag="PO783_RS30435"
                     /old_locus_tag="PO783_30435"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(627..785)
                     /locus_tag="PO783_RS30640"
     CDS             complement(627..785)
                     /locus_tag="PO783_RS30640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1425..1601
                     /locus_tag="PO783_RS30440"
                     /old_locus_tag="PO783_30440"
     CDS             1425..1601
                     /locus_tag="PO783_RS30440"
                     /old_locus_tag="PO783_30440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            1591..1935
                     /locus_tag="PO783_RS30445"
                     /old_locus_tag="PO783_30445"
     CDS             1591..1935
                     /locus_tag="PO783_RS30445"
                     /old_locus_tag="PO783_30445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2144..>2578
                     /locus_tag="PO783_RS30450"
                     /old_locus_tag="PO783_30450"
                     /pseudo=""
     CDS             2144..>2578
                     /locus_tag="PO783_RS30450"
                     /old_locus_tag="PO783_30450"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2613..3122
                     /locus_tag="PO783_RS30455"
                     /old_locus_tag="PO783_30455"
     CDS             2613..3122
                     /locus_tag="PO783_RS30455"
                     /old_locus_tag="PO783_30455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            3129..3341
                     /locus_tag="PO783_RS30460"
                     /old_locus_tag="PO783_30460"
     CDS             3129..3341
                     /locus_tag="PO783_RS30460"
                     /old_locus_tag="PO783_30460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4008..4232
                     /locus_tag="PO783_RS30465"
                     /old_locus_tag="PO783_30465"
     CDS             4008..4232
                     /locus_tag="PO783_RS30465"
                     /old_locus_tag="PO783_30465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            4216..4482
                     /locus_tag="PO783_RS30470"
                     /old_locus_tag="PO783_30470"
     CDS             4216..4482
                     /locus_tag="PO783_RS30470"
                     /old_locus_tag="PO783_30470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4552..4902)
                     /locus_tag="PO783_RS30475"
                     /old_locus_tag="PO783_30475"
     CDS             complement(4552..4902)
                     /locus_tag="PO783_RS30475"
                     /old_locus_tag="PO783_30475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(4968..5513)
                     /locus_tag="PO783_RS30480"
                     /old_locus_tag="PO783_30480"
     CDS             complement(4968..5513)
                     /locus_tag="PO783_RS30480"
                     /old_locus_tag="PO783_30480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(5539..5865)
                     /locus_tag="PO783_RS30485"
                     /old_locus_tag="PO783_30485"
     CDS             complement(5539..5865)
                     /locus_tag="PO783_RS30485"
                     /old_locus_tag="PO783_30485"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(6223..6381)
                     /locus_tag="PO783_RS30645"
     CDS             complement(6223..6381)
                     /locus_tag="PO783_RS30645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            7021..7197
                     /locus_tag="PO783_RS30490"
                     /old_locus_tag="PO783_30490"
     CDS             7021..7197
                     /locus_tag="PO783_RS30490"
                     /old_locus_tag="PO783_30490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            7187..7531
                     /locus_tag="PO783_RS30495"
                     /old_locus_tag="PO783_30495"
     CDS             7187..7531
                     /locus_tag="PO783_RS30495"
                     /old_locus_tag="PO783_30495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7740..>8174
                     /locus_tag="PO783_RS30500"
                     /old_locus_tag="PO783_30500"
                     /pseudo=""
     CDS             7740..>8174
                     /locus_tag="PO783_RS30500"
                     /old_locus_tag="PO783_30500"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            8209..8718
                     /locus_tag="PO783_RS30505"
                     /old_locus_tag="PO783_30505"
     CDS             8209..8718
                     /locus_tag="PO783_RS30505"
                     /old_locus_tag="PO783_30505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            8725..8937
                     /locus_tag="PO783_RS30510"
                     /old_locus_tag="PO783_30510"
     CDS             8725..8937
                     /locus_tag="PO783_RS30510"
                     /old_locus_tag="PO783_30510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            9604..9828
                     /locus_tag="PO783_RS30515"
                     /old_locus_tag="PO783_30515"
     CDS             9604..9828
                     /locus_tag="PO783_RS30515"
                     /old_locus_tag="PO783_30515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            9812..10078
                     /locus_tag="PO783_RS30520"
                     /old_locus_tag="PO783_30520"
     CDS             9812..10078
                     /locus_tag="PO783_RS30520"
                     /old_locus_tag="PO783_30520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(10148..10498)
                     /locus_tag="PO783_RS30525"
                     /old_locus_tag="PO783_30525"
     CDS             complement(10148..10498)
                     /locus_tag="PO783_RS30525"
                     /old_locus_tag="PO783_30525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(10564..11109)
                     /locus_tag="PO783_RS30530"
                     /old_locus_tag="PO783_30530"
     CDS             complement(10564..11109)
                     /locus_tag="PO783_RS30530"
                     /old_locus_tag="PO783_30530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     Typing          1260..1339
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          6856..6935
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(677..1074)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(6273..6670)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          1926..3457
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7522..9053
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttaataattg aatctatctc atccattttt tcttggtatt cttttaatgg tgaagggagc
       61 ggtaactcaa tatttgaaaa gtgattggcg agatagggat caaggtttct catccattca
      121 ggcttttcgc caatgcgttc ttcaactgaa agtgaacctg ataatctctg gttaataaga
      181 tcacttagac tcattttggt tttattcgaa agccgttgta aggattgctt taatcctata
      241 tctagcatga tatttgttct ttctctcatt accccccctt gctattgcgt tttttgttgc
      301 gcacaaagct accattgcgc attaagaaac gcaataaaaa aggagggagg gagtggtttg
      361 tacatgatgt gcagtgcgct gagggcatca gtggcgggtg tttttccgtg ggatggatgg
      421 agctccgggg ataacagaac tgacgatcat ttaaaggatc ttcttgagat cctttttttc
      481 tgcgcgtaat ctcttgcact gtaaacgaaa aaaccgcctg gggaggcggt ttgatcgaag
      541 gttaagtcag ttggggaact gcttaaccgg gtaactgggt atacgagacc gcagatacca
      601 aaactgttct ttcagtgtag ccgcagttag gcacccactt caagacctct caatatctct
      661 ctcgtatata tccagttacc aatggctgct gccagtggcg tttttgcgtg tccgtccggg
      721 ttggactcaa gatgatagtt accggaaagg gcgcagcagt cgggctgaac ggggggttcg
      781 tgcatacagc ccagcttgga gcgaacgacc taaaccgagc cgagatacca gcagcgtgag
      841 ctatgagaaa gcgccacgct tcccgaaagg gaaaaaggcg gaacaggtat ccggtaaacg
      901 gcagggtcgg aacaggagag cgcacgaggg agccgccgga gagaaatcac cggcatcttt
      961 aagtcctgtc gggtttcgtc ctcctctgac ttgagcgtcc atttctgtga tgctcgtcag
     1021 gggaggcgga gcctatggaa aaacggcaac gccgcatcct tttctccggc cctttctgat
     1081 ttgagcgcct ttgctttatc ccgttatccg tgagtcggtc acgcccgccg cagccgaacg
     1141 gcaggagcgc agctaccgag tgagcgagga ggcgtcattt catctgtccg tgcatggtat
     1201 tgcggacgct cctgagccac atatcacacc tgccacatga ggcactttca tgaatcgtgc
     1261 tctcatgcca acatagtaag ccagtataca cagcgtaaac gctgtgactg gttcagggct
     1321 gcgccccgaa acccgctaac accactgacg cgctggcgct tgtccaacgc cggagcgacc
     1381 cggaaaactt ttccgtgccg tccggcgttt gcgaaggagg gataatgcag aagccgaaga
     1441 agaagccgct cgtcgtaaaa aaaagatcct ggcatccggc agtgatggca aatctgatgc
     1501 gtcaggcaca ggaggaagat taccgccggg agcagatggc agagcttgag cgttcagccc
     1561 ggtcacattt tgcggatatc ggaggagagg atggcggtta agcgtacccg ttttttaggt
     1621 atccgggtga ccgatgggga gtaccagcag ctgctggagc gctgcaatgg aaggcaactg
     1681 gcggtgtgga tgcgggaaac ctgtctcgat acgcgcccgg cgcggtcgtt gcggctgccg
     1741 tccatcgatc cggttttgct gcgccagctt gccgggatgg ggaacaacct caaccagatc
     1801 gcccggaaaa ttaacggcgg ccagtggtca ggcgctgacc gggttcaggt ggtggccgcg
     1861 ctgatggcca ttgatgccga actcgagcgg ctgcggcatg ccgtgctgga aaagggggca
     1921 gacgatgatc gttaagtttc atccgcgagg gcgaggcggc ggtgccgggc cggtggatta
     1981 tctgctgggg aaagaccgcc agcgcgacgg ggccatcgtc ctgcagggaa agccggagga
     2041 ggtccgggag cttatcgacg cctcgcccta cgccaaaaag tacacctccg gcgtcctgtc
     2101 ttttgctgaa caggatttac cgcccggcca gcgtgaaaag ctgatggcga gcttcgagcg
     2161 ggttctgatg cccggactcg ataaagacca gtacagcgtg ctgtgggttg aacaccggga
     2221 caaggggcgg ctggaactga attttctggt accgaacacg gaactgctga ccggcaggcg
     2281 tctccagccg tactacgacc gggcagaccg tccgcgcatc gatgcgtggc agaccgtggt
     2341 gaacgggcga ctggggctgc acgacccgaa cgcaccggag aaccggcgcg cgctggtgac
     2401 gccatcttcg ttacccaaaa cgaagcagga agccgcagag gctattacgc ggggcttact
     2461 ggccctcgcc tcgtcagggg agcttaaaac gcgtcaggac gtcactgagg cgctggaaag
     2521 cgcaggtttt gaggtcgtgc gcaccacaaa gagcagcatc agcattgccg acccggacgg
     2581 agggcgaaac atccgactta agggagccat ctatgaacag tcttttaacg ctggcgaagg
     2641 acttagagca gaaatcgaaa gcgcagcagc agagtaccgg cgaaatgctg aaagccgcat
     2701 tcagcgagca cgagaagtct gtcagagcgg aactgagcga aagcgggaag agaatcagcg
     2761 ccgccatccg cgcccacgag cagggtatga gcgcggccat gcagtcgaac cgcctgagcg
     2821 tgatgcgtat ggtcgggcgg acatggctga ccatcacgat ggtgtcagga ctgctgttcg
     2881 ccagcctgag cggggtgttg tggtatcagg ggagcttgat agcgtcaaat ctggccgaaa
     2941 tcgaccggca gaacgcagcg ctgtcgaagc tgaacgcgaa gacctggggc gtgacgtacc
     3001 tggaagacag caacggacgt tttctggtgc tgccgaaggg gacggccgta gacaggacgc
     3061 agagctggac ggtcgggaac gggcggtcga agcagaacgc gctgagactg gtgaaggagt
     3121 aagtgccgat gaccgagctg gaaaaacagt tgctgagcgc attagagcag ctacagcagg
     3181 actactcgaa aaggctggac gagtgggaga gcgccttcgg ggaatggcgg aaaatgtcag
     3241 ggcttatgca acgggagaac gcggcgctga gcgagcgcgt gacgcgcttg agtcagcagg
     3301 tggaacgctt gagcgggcaa ctgcagcgtt tgagccggta gtgcagcgtc atgagatggc
     3361 cgtggccgca gaacgggcgc acgagcaacg gcagcacgaa aaggaactga cggaagcgcg
     3421 ttcccggcag cgaagctatg acggtccgtc gctgggctga aatgcaggaa aacagggttc
     3481 tcatcgggca tttacgctga aaccggatgt cattcacctg gctgcatggc tatgcagcca
     3541 ggtaaaattt atctgtcgcg aacggggcgg tttatcgggt ggtttgttgc cggttttacc
     3601 tgactgccac cccgttcgcg gcgaacccgt ccggggcggt gcgggcaacg ggtgttatgt
     3661 ttaatcagcg tgaacggcag atacaaaaaa gccccggagg gcttttaact actgacttat
     3721 ttttttgaag atttgctggg tttcaatttg tcagtatttc ccggcgtttt tttattgtca
     3781 cgctggcgtt tatcaggttc ccctgttgaa tcggcaattc gtttcggacc cggcttaaga
     3841 cccataatcg ttatcctttc aatagttaag aggaaagccc tcaagctaga tatattgacc
     3901 gcgttacgga ttatcaagag gggctaacaa gattattttt tgttctttta ggttgattgt
     3961 ggtactgttg atatacatgt atagcaaatg gtgttcggga ggtttatatg cttgctatcc
     4021 ggttatctga tgagattgag tcccgtctgg actcgctggc gaagcaaacc ggcagaacaa
     4081 agacgtttta tgcgcgggaa gcaatactgg cgcatctgga agacctggag gattattatc
     4141 tttcagcaga aactgctgca cgcgttcgcc gtggtgatga agcagtgcat tcgtctgaag
     4201 acgtgaggaa gtcacttggt ctggacgatt aactattccg atcgggcgct caaatcgtta
     4261 cgcaagatgg acaaacagaa cgcacgacgg attgtggatt ttatgagttt acgcattgca
     4321 gttgctgccg atcctcgcca gtcagggaag ccgctcaaag gtgagctggg cgagttctgg
     4381 cgctatcggg tgggagatta tcgcgttctg tgtgagatcc gagatgacga gcttgttatc
     4441 cttgccgcca cgattggaca tcgccgcgaa gtttacgact gaagcccgct atgcgggctt
     4501 ttttgatgcc gacaacaggt attatggtaa atactcgact aactacgtgt tctactgttt
     4561 gtcggtttta gttaaccagc aggttgtctt aacgataatg tagccgttat ttttaactaa
     4621 taaggtcata ttgttaacgt caggcataat aaaagacatt atggcctcag agttatcatc
     4681 agaagcagct ctctgcatta ctacttcgat tcccttttct ttgaatctat aagtatccag
     4741 ttgcctatta aacttagaaa gacctacaac catacttagt ttgttatctt taacagtaag
     4801 agatgcttcc tgagtaccat tacatcggta gtaagcggtg taaccgtcag caaaagatcg
     4861 accggggaat ataaatgcta ggaacgcaat taaccaatac atagttgaga atcttggtat
     4921 tacacatttc actatcctct ctttcatttt tcgttcacca tactttgtta tctgattttt
     4981 aatttttcta ttttataaac caagtctcta ccaaaaaggc gaaaattatc ctcaaataat
     5041 aataattgtt taagaacatc catgaaagca gctgcttcac cctcagttat gggagctact
     5101 ctatataaca tataacctga gttaagggtt tcttttatac tttcttcaat gctcattata
     5161 aatattagtg gattatcatt tttcttcccc catgggtttc taattaatcc ttcgatgtac
     5221 tcaggtcctt tgcgtcctat tgcctgcgca atatttttta tttcatctgc gtgagggctt
     5281 tgttgtacag ctttgtttat gcctgcttga gtgattttca gagactctac accgtgtatg
     5341 ttgtatgaga taaaatgtaa taaattaaga tgcaatgcaa ttaatccatt tttgtttaca
     5401 tctgagtatt tggataaaaa acgtaatgaa gtgttaactc tcccttgaag cctaacaatt
     5461 gggagtgctg ccttgtatat ttccttctcc gttaaatacc gtttaaaaaa cataattctc
     5521 cgcctccgta tagcgtgcct attttatttc gtcgtaaaac ttagctctga aatattcctt
     5581 tagaaccatc atgtcattaa taattgaatc tatctcatcc attttttctt ggtattcttt
     5641 taatggtgaa gggagcggta actcaatatt tgaaaagtga ttggcgagat agggatcaag
     5701 gtttctcatc cattcaggct tttcgccaat gcgttcttca actgaaagtg aacctgataa
     5761 tctctggtta ataagatcac ttagactcat tttggtttta ttcgaaagcc gttgtaagga
     5821 ttgctttaat cctatatcta gcatgatatt tgttctttct ctcattaccc ccccttgcta
     5881 ttgcgttttt tgttgcgcac aaagctacca ttgcgcatta agaaacgcaa taaaaaagga
     5941 gggagggagt ggtttgtaca tgatgtgcag tgcgctgagg gcatcagtgg cgggtgtttt
     6001 tccgtgggat ggatggagct ccggggataa cagaactgac gatcatttaa aggatcttct
     6061 tgagatcctt tttttctgcg cgtaatctct tgcactgtaa acgaaaaaac cgcctgggga
     6121 ggcggtttga tcgaaggtta agtcagttgg ggaactgctt aaccgggtaa ctgggtatac
     6181 gagaccgcag ataccaaaac tgttctttca gtgtagccgc agttaggcac ccacttcaag
     6241 acctctcaat atctctctcg tatatatcca gttaccaatg gctgctgcca gtggcgtttt
     6301 tgcgtgtccg tccgggttgg actcaagatg atagttaccg gaaagggcgc agcagtcggg
     6361 ctgaacgggg ggttcgtgca tacagcccag cttggagcga acgacctaaa ccgagccgag
     6421 ataccagcag cgtgagctat gagaaagcgc cacgcttccc gaaagggaaa aaggcggaac
     6481 aggtatccgg taaacggcag ggtcggaaca ggagagcgca cgagggagcc gccggagaga
     6541 aatcaccggc atctttaagt cctgtcgggt ttcgtcctcc tctgacttga gcgtccattt
     6601 ctgtgatgct cgtcagggga ggcggagcct atggaaaaac ggcaacgccg catccttttc
     6661 tccggccctt tctgatttga gcgcctttgc tttatcccgt tatccgtgag tcggtcacgc
     6721 ccgccgcagc cgaacggcag gagcgcagct accgagtgag cgaggaggcg tcatttcatc
     6781 tgtccgtgca tggtattgcg gacgctcctg agccacatat cacacctgcc acatgaggca
     6841 ctttcatgaa tcgtgctctc atgccaacat agtaagccag tatacacagc gtaaacgctg
     6901 tgactggttc agggctgcgc cccgaaaccc gctaacacca ctgacgcgct ggcgcttgtc
     6961 caacgccgga gcgacccgga aaacttttcc gtgccgtccg gcgtttgcga aggagggata
     7021 atgcagaagc cgaagaagaa gccgctcgtc gtaaaaaaaa gatcctggca tccggcagtg
     7081 atggcaaatc tgatgcgtca ggcacaggag gaagattacc gccgggagca gatggcagag
     7141 cttgagcgtt cagcccggtc acattttgcg gatatcggag gagaggatgg cggttaagcg
     7201 tacccgtttt ttaggtatcc gggtgaccga tggggagtac cagcagctgc tggagcgctg
     7261 caatggaagg caactggcgg tgtggatgcg ggaaacctgt ctcgatacgc gcccggcgcg
     7321 gtcgttgcgg ctgccgtcca tcgatccggt tttgctgcgc cagcttgccg ggatggggaa
     7381 caacctcaac cagatcgccc ggaaaattaa cggcggccag tggtcaggcg ctgaccgggt
     7441 tcaggtggtg gccgcgctga tggccattga tgccgaactc gagcggctgc ggcatgccgt
     7501 gctggaaaag ggggcagacg atgatcgtta agtttcatcc gcgagggcga ggcggcggtg
     7561 ccgggccggt ggattatctg ctggggaaag accgccagcg cgacggggcc atcgtcctgc
     7621 agggaaagcc ggaggaggtc cgggagctta tcgacgcctc gccctacgcc aaaaagtaca
     7681 cctccggcgt cctgtctttt gctgaacagg atttaccgcc cggccagcgt gaaaagctga
     7741 tggcgagctt cgagcgggtt ctgatgcccg gactcgataa agaccagtac agcgtgctgt
     7801 gggttgaaca ccgggacaag gggcggctgg aactgaattt tctggtaccg aacacggaac
     7861 tgctgaccgg caggcgtctc cagccgtact acgaccgggc agaccgtccg cgcatcgatg
     7921 cgtggcagac cgtggtgaac gggcgactgg ggctgcacga cccgaacgca ccggagaacc
     7981 ggcgcgcgct ggtgacgcca tcttcgttac ccaaaacgaa gcaggaagcc gcagaggcta
     8041 ttacgcgggg cttactggcc ctcgcctcgt caggggagct taaaacgcgt caggacgtca
     8101 ctgaggcgct ggaaagcgca ggttttgagg tcgtgcgcac cacaaagagc agcatcagca
     8161 ttgccgaccc ggacggaggg cgaaacatcc gacttaaggg agccatctat gaacagtctt
     8221 ttaacgctgg cgaaggactt agagcagaaa tcgaaagcgc agcagcagag taccggcgaa
     8281 atgctgaaag ccgcattcag cgagcacgag aagtctgtca gagcggaact gagcgaaagc
     8341 gggaagagaa tcagcgccgc catccgcgcc cacgagcagg gtatgagcgc ggccatgcag
     8401 tcgaaccgcc tgagcgtgat gcgtatggtc gggcggacat ggctgaccat cacgatggtg
     8461 tcaggactgc tgttcgccag cctgagcggg gtgttgtggt atcaggggag cttgatagcg
     8521 tcaaatctgg ccgaaatcga ccggcagaac gcagcgctgt cgaagctgaa cgcgaagacc
     8581 tggggcgtga cgtacctgga agacagcaac ggacgttttc tggtgctgcc gaaggggacg
     8641 gccgtagaca ggacgcagag ctggacggtc gggaacgggc ggtcgaagca gaacgcgctg
     8701 agactggtga aggagtaagt gccgatgacc gagctggaaa aacagttgct gagcgcatta
     8761 gagcagctac agcaggacta ctcgaaaagg ctggacgagt gggagagcgc cttcggggaa
     8821 tggcggaaaa tgtcagggct tatgcaacgg gagaacgcgg cgctgagcga gcgcgtgacg
     8881 cgcttgagtc agcaggtgga acgcttgagc gggcaactgc agcgtttgag ccggtagtgc
     8941 agcgtcatga gatggccgtg gccgcagaac gggcgcacga gcaacggcag cacgaaaagg
     9001 aactgacgga agcgcgttcc cggcagcgaa gctatgacgg tccgtcgctg ggctgaaatg
     9061 caggaaaaca gggttctcat cgggcattta cgctgaaacc ggatgtcatt cacctggctg
     9121 catggctatg cagccaggta aaatttatct gtcgcgaacg gggcggttta tcgggtggtt
     9181 tgttgccggt tttacctgac tgccaccccg ttcgcggcga acccgtccgg ggcggtgcgg
     9241 gcaacgggtg ttatgtttaa tcagcgtgaa cggcagatac aaaaaagccc cggagggctt
     9301 ttaactactg acttattttt ttgaagattt gctgggtttc aatttgtcag tatttcccgg
     9361 cgttttttta ttgtcacgct ggcgtttatc aggttcccct gttgaatcgg caattcgttt
     9421 cggacccggc ttaagaccca taatcgttat cctttcaata gttaagagga aagccctcaa
     9481 gctagatata ttgaccgcgt tacggattat caagaggggc taacaagatt attttttgtt
     9541 cttttaggtt gattgtggta ctgttgatat acatgtatag caaatggtgt tcgggaggtt
     9601 tatatgcttg ctatccggtt atctgatgag attgagtccc gtctggactc gctggcgaag
     9661 caaaccggca gaacaaagac gttttatgcg cgggaagcaa tactggcgca tctggaagac
     9721 ctggaggatt attatctttc agcagaaact gctgcacgcg ttcgccgtgg tgatgaagca
     9781 gtgcattcgt ctgaagacgt gaggaagtca cttggtctgg acgattaact attccgatcg
     9841 ggcgctcaaa tcgttacgca agatggacaa acagaacgca cgacggattg tggattttat
     9901 gagtttacgc attgcagttg ctgccgatcc tcgccagtca gggaagccgc tcaaaggtga
     9961 gctgggcgag ttctggcgct atcgggtggg agattatcgc gttctgtgtg agatccgaga
    10021 tgacgagctt gttatccttg ccgccacgat tggacatcgc cgcgaagttt acgactgaag
    10081 cccgctatgc gggctttttt gatgccgaca acaggtatta tggtaaatac tcgactaact
    10141 acgtgttcta ctgtttgtcg gttttagtta accagcaggt tgtcttaacg ataatgtagc
    10201 cgttattttt aactaataag gtcatattgt taacgtcagg cataataaaa gacattatgg
    10261 cctcagagtt atcatcagaa gcagctctct gcattactac ttcgattccc ttttctttga
    10321 atctataagt atccagttgc ctattaaact tagaaagacc tacaaccata cttagtttgt
    10381 tatctttaac agtaagagat gcttcctgag taccattaca tcggtagtaa gcggtgtaac
    10441 cgtcagcaaa agatcgaccg gggaatataa atgctaggaa cgcaattaac caatacatag
    10501 ttgagaatct tggtattaca catttcacta tcctctcttt catttttcgt tcaccatact
    10561 ttgttatctg atttttaatt tttctatttt ataaaccaag tctctaccaa aaaggcgaaa
    10621 attatcctca aataataata attgtttaag aacatccatg aaagcagctg cttcaccctc
    10681 agttatggga gctactctat ataacatata acctgagtta agggtttctt ttatactttc
    10741 ttcaatgctc attataaata ttagtggatt atcatttttc ttcccccatg ggtttctaat
    10801 taatccttcg atgtactcag gtcctttgcg tcctattgcc tgcgcaatat tttttatttc
    10861 atctgcgtga gggctttgtt gtacagcttt gtttatgcct gcttgagtga ttttcagaga
    10921 ctctacaccg tgtatgttgt atgagataaa atgtaataaa ttaagatgca atgcaattaa
    10981 tccatttttg tttacatctg agtatttgga taaaaaacgt aatgaagtgt taactctccc
    11041 ttgaagccta acaattggga gtgctgcctt gtatatttcc ttctccgtta aataccgttt
    11101 aaaaaacata attctccgcc tccgtatagc gtgcctattt tatttcgtcg taaaacttag
    11161 ctctgaaata ttcctttaga accatcatgt ca
//
