LOCUS       NZ_CP116535            12989 bp    DNA     linear   CON 01-MAR-2024
DEFINITION  Enterococcus faecium strain K191-3 plasmid unnamed.
ACCESSION   NZ_CP116535
VERSION     NZ_CP116535.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23424529
            Assembly: GCF_028335225.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12989)
  AUTHORS   Biggel,M.
  TITLE     Oxazolidinone resistance genes in florfenicol resistant enterococci
            from beef cattle and veal calves at slaughter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12989)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2023) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x, 50x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/01/2024 01:48:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,799
            CDSs (total)                      :: 2,709
            Genes (coding)                    :: 2,619
            CDSs (with protein)               :: 2,619
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 90
            CDSs (without protein)            :: 90
            Pseudo Genes (ambiguous residues) :: 0 of 90
            Pseudo Genes (frameshifted)       :: 36 of 90
            Pseudo Genes (incomplete)         :: 47 of 90
            Pseudo Genes (internal stop)      :: 24 of 90
            Pseudo Genes (multiple problems)  :: 14 of 90
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116535.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..12989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="K191-3"
                     /isolation_source="fecal sample"
                     /host="Bos taurus"
                     /db_xref="taxon:1352"
                     /plasmid="unnamed"
                     /collection_date="2021"
                     /collected_by="ILS, University of Zurich"
     gene            complement(154..429)
                     /gene="prgO"
                     /locus_tag="PML76_RS13890"
                     /old_locus_tag="PML76_13890"
     CDS             complement(154..429)
                     /gene="prgO"
                     /locus_tag="PML76_RS13890"
                     /old_locus_tag="PML76_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="WP_001196543.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVDP
                     PVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
                     /name="prgO"
     gene            complement(401..1354)
                     /locus_tag="PML76_RS13895"
                     /old_locus_tag="PML76_13895"
     CDS             complement(401..1354)
                     /locus_tag="PML76_RS13895"
                     /old_locus_tag="PML76_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_000429439.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLFE
                     KDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGSPG
                     SEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAADYII
                     AMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQKTID
                     KYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYYEQHGD
                     IEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
                     /name="ParA family protein"
     gene            1966..3459
                     /locus_tag="PML76_RS13900"
                     /old_locus_tag="PML76_13900"
     CDS             1966..3459
                     /locus_tag="PML76_RS13900"
                     /old_locus_tag="PML76_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="WP_000947691.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSKQ
                     LMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINTFV
                     VDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVIDVA
                     KKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSMKLES
                     DQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTLSLAYY
                     SSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELCQQWVSP
                     TLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLNLSKKELA
                     SQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLSKEKRLDYS
                     TRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
                     /name="primase C-terminal domain-containing protein"
     gene            3594..3890
                     /locus_tag="PML76_RS13905"
                     /old_locus_tag="PML76_13905"
     CDS             3594..3890
                     /locus_tag="PML76_RS13905"
                     /old_locus_tag="PML76_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication control protein PrgN"
                     /protein_id="WP_000053907.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTRE
                     KTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
                     /name="replication control protein PrgN"
     gene            3991..4842
                     /locus_tag="PML76_RS13910"
                     /old_locus_tag="PML76_13910"
     CDS             3991..4842
                     /locus_tag="PML76_RS13910"
                     /old_locus_tag="PML76_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002287227.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILDL
                     TVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAGKL
                     FKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILFFVG
                     YVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKGIRSI
                     LKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDLELPMK
                     DK"
                     /name="hypothetical protein"
     gene            4863..5075
                     /locus_tag="PML76_RS13915"
                     /old_locus_tag="PML76_13915"
     CDS             4863..5075
                     /locus_tag="PML76_RS13915"
                     /old_locus_tag="PML76_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002324171.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIEY
                     RTEYLELTNQTDFLLIRKQQEQEKN"
                     /name="hypothetical protein"
     gene            5508..6188
                     /locus_tag="PML76_RS13920"
                     /old_locus_tag="PML76_13920"
     CDS             5508..6188
                     /locus_tag="PML76_RS13920"
                     /old_locus_tag="PML76_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            complement(<6234..6572)
                     /locus_tag="PML76_RS13925"
                     /old_locus_tag="PML76_13925"
                     /pseudo=""
     CDS             complement(<6234..6572)
                     /locus_tag="PML76_RS13925"
                     /old_locus_tag="PML76_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            complement(6817..8079)
                     /gene="mobV"
                     /locus_tag="PML76_RS13930"
                     /old_locus_tag="PML76_13930"
     CDS             complement(6817..8079)
                     /gene="mobV"
                     /locus_tag="PML76_RS13930"
                     /old_locus_tag="PML76_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000119405.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYD
                     LKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFE
                     ESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKF
                     PEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVK
                     SDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDND
                     RLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSK
                     ISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNE
                     FELTHNREVKKERSRDQGMSL"
                     /name="mobV"
     gene            complement(8643..10019)
                     /gene="tet(L)"
                     /locus_tag="PML76_RS13935"
                     /old_locus_tag="PML76_13935"
     ARG             complement(8647..10019)
                     /name="tet(L)"
                     /gene="tet(L)"
                     /gene_name="tetracycline efflux MFS transporter Tet(L)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="95.41"
                     /coverage="100.0"
     gene            complement(10218..12137)
                     /gene="tet(M)"
                     /locus_tag="PML76_RS13940"
                     /old_locus_tag="PML76_13940"
     ARG             complement(10222..12137)
                     /name="tet(M)"
                     /gene="tet(M)"
                     /gene_name="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="98.12"
                     /coverage="100.0"
     gene            complement(12153..12269)
                     /locus_tag="PML76_RS13945"
                     /old_locus_tag="PML76_13945"
     CDS             complement(12153..12269)
                     /locus_tag="PML76_RS13945"
                     /old_locus_tag="PML76_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="WP_001814923.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
                     /name="tetracycline resistance determinant leader peptide"
     gene            complement(12514..>12989)
                     /locus_tag="PML76_RS13950"
                     /old_locus_tag="PML76_13950"
     CDS             complement(12514..>12989)
                     /locus_tag="PML76_RS13950"
                     /old_locus_tag="PML76_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="WP_272025093.1"
                     /translation="TYSVDQLITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIP
                     KKSSYKPKAIESEGTVDSITTNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEY
                     IFQELVNPIHNRKDNQVTVSLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
                     /name="conjugal transfer protein"
     ARG             complement(8644..9929)
                     /name="tet(45)"
                     /gene="tet(45)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="77.75"
                     /coverage="93.45"
     Typing          1967..3459
                     /name="rep_cluster_185"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6821..8079)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.19"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atatttagta tagtaaaaac tagaaacaat aaaatttgtg aatgttgtat acccccaaac
       61 aatacacata ttttgaagga ttaggattaa taaagctaaa aatatagtaa gtatactaaa
      121 tatatttact atatctagta tacttactat attttaatct tttgatcgtc tattatatat
      181 aatccgttcc tcgtcgttta aagtatcaac ataggcgtcc attgcaagtt tgactatttc
      241 atacatagga atatcttttg cataactaag atcccgaatg gtttttagta cgggaggatc
      301 tacttgaact ggttttcttt ctttcgcagt gtactttcgt tcccctaact cgacattaat
      361 atcatcttta attgaaatat cttcagcacc aattttcttc ttatctttgt tcgttggtaa
      421 taatgccatt tagtttaatc tcctttccaa gaggtaggat atgattggcc attgagtcag
      481 catatttagg tgtataatca aatccttcaa tatcaccatg ttgttcatag tattcaattc
      541 tttcttctaa ttctgtaaag atgtctgcaa aaatagccca ccatcttcga tctacataat
      601 catttaattg gataccattc tcaccataca tttctaaccg atccgagcct ttaacaatgc
      661 ttttaaaaat gtttgattca ccatacttat caattgtttt ttgatagttt gcgtgttgag
      721 aggaacgtcg cactgagaaa agaacaggca aaattccaat gatttgaaga tgagactctt
      781 caaaattcgt gacaatcgga actaaaactt tattaataaa atcttctgtt ccttccattg
      841 cgtaccgttt taattcttgc attgcaataa tataatctgc agcagttaag aatgcacgaa
      901 ccacattatc tgttgatggt gggcagtcga taaagatata atcgaaattt tttctcaatt
      961 cttctaaagg tttaatgaag gccaatgatc tcttacgacg atcaggatat ttttcggaca
     1021 agtattcatt aagttcttca gatccaggag agccagccat aatatataaa tgtggactta
     1081 cttgagtaat acctgaaacc cagtcccctt ttttgattgc tgcagacatg gatcgatcaa
     1141 agctagtaat ttgatacgtt gatccaatgt tggaagtagt atttctttgt tggtcataat
     1201 caattagcaa cacgtctttt tcaaaaagtt gactagaaac aatcgctaat aaatcattgt
     1261 ctgtagtctt accaatgccg ccttttctac ctgtaagcat tacaactttt cctagtttat
     1321 ctgatgtttt aaatgcagtc cgtaagtttt ccatagttcc ctccaatttc ctatactaaa
     1381 tatatttagt atacttcata tacttaatat attcgatata tctaatatac tccaaattga
     1441 taagaattca gactttataa ggaatatagt ggatatacta aatatatttg ctatattcag
     1501 tatatcagat atattcaaaa tgtcaaaata tatttgtgcg ttttgcgttg acaatttaga
     1561 gtgaaaagag gtaaacttaa ttctatagaa accacagata attcactatt tacaaagtag
     1621 caaaagcagt ggtactcttt aaacagaaaa attacataaa aaaaagagac ggaaataatt
     1681 gattttagca gaatcaatta tttctgaatc acatggttag gacatgtgtt cacgctcgtc
     1741 tcaagaatat gtaagtagct accaactact tatattaaaa gttgatcgga taccttttcc
     1801 gattgccttt attctaccaa aaaaaggcga gcaactcaac agaagaaagt ttatttagtg
     1861 tacttaaatt aaatattctt aaacactgaa gagagaacac atactatggc tgagattaaa
     1921 gctaaactgt gttctctttt tgtttgagaa aggacgttac aatcgatgtt gagcgaaatt
     1981 tattcagcta ttcttaaaaa cggcttacgt acatacaaat atcgaaacag caccatgaaa
     2041 cctgcaggtc atattgattc cagtaggagt ggtgctatct ttgcgtaccg tagcaagcaa
     2101 ttaatgaatg ttgggcgtgg tatggtcatt acttcggaag aagctatttt agagaacgaa
     2161 aagaaactaa cgcattggac acctaatacc tatcgctttg gtacttatgc ggatgattat
     2221 cgaacagtgg ttaaaggtca ttctgagaaa aacttaagtc aaatcaacac atttgtagtt
     2281 gatatcgatt caaaagagaa ccatcaaggc gaaattattt tagcctgttt agatcaagtg
     2341 ggctacatgc caacgctaat tttagaatca gatcatggtt atcaagttta ttttgtacta
     2401 aagacaccag gctatgtcac aaagaaaagc aatttcaagg tgatcgatgt agcaaaaaaa
     2461 atttcaaaaa caattcgtct acagttggct gaaaaattgt ccggagtgga tctaggatgt
     2521 aaccacttcg gaattgctcg attcccacaa gaaaataata ttgtttttaa agaaatgaac
     2581 tatcagtatt cttttgctga ctggattcaa tggtcgatga agcttgaatc agatcaaaaa
     2641 acaaaagaag atcgagaacg aaaactaatt gtctttcctg agaaaaaaga aattcgtcaa
     2701 gtagatgaac cgtggttcga tatgttactt cataaagcaa acatcgtcgg cggagaagga
     2761 cgtcttggtc gaaataatgt gattttcacg ctttctttag cttattactc ttcaggttat
     2821 gcattggata cttgcgaata caatatgttc acattcaacg aacggttaca ggaaccgcta
     2881 tctgaagcag aattagtaaa gattgtacgt agtgcttatt ctggtaatta tcaagcagca
     2941 agcagagaat tcattattga attgtgccag caatgggttt caccaacctt aaaaaataat
     3001 gatctgttca ttcaacgacg tggctggtgg aaatttaaaa agccacgaga tcaaagaaaa
     3061 tatagtcata aaaatgaatg gaaagaagat ctattggctt atctaagcga aaaaagtctt
     3121 gctagtgagc cttatttgaa cctgtcaaaa aaagaattgg cttcacaact aacgatgccc
     3181 aagcgaacac ttgataagat cttaaatgag ttacgagagg agaataaagt cttctacaag
     3241 gtaacacgag gaagaaatgg cggaatgatc attgcttcag tcaaaacgtt gttcgcgagg
     3301 gttattcaac taagtaaaga aaagagacta gattattcaa caaggatcac agaggtattt
     3361 ggactcaacg agagcttagt agaagagaca tttagacaat tatcaacccc cttgaaccct
     3421 cttaaagaag tgaatctttt tgaagtagat acaggttgaa aaataaaagg tgcaaattgc
     3481 cctatacaat atatctatga tacttctgta ggtgttgctc cctttgccta gctacgtgta
     3541 ggtggtatac ttataactgc gtatgcagtc ttatgtatca tggaggaagt tgtatgtcgt
     3601 taaaaaatta tgtctattca catcctgtta atgtgtttat cattggtcgt ttgggattat
     3661 ctgtggaagt gttctgtgag ctttatggtt ttaagcaggg gactttgtct tcttgggtta
     3721 ccagggaaaa aacagttgct tctttgccga ttgagtttct tcatgggttg tccttagctt
     3781 ctggggagac tatggatcag gtatatgatt gtttgtgtgt gttagagcag gagtatattg
     3841 agtataaaat agctaacgag ttaagaaaaa ggaagaagta tatccagtag tagtttttat
     3901 ttacttaaaa aattggttca ttgagcaacc tatcgctgct aaagtagcaa aggaaaacgt
     3961 taaaggaaag aatttaggga agaaatcgaa atgaaatcgg acgactggat tgaacacacc
     4021 tggcttcctc tgaaaagaaa tagcaaacga aagaaatttt gggtattgca attatggcca
     4081 ttaggaaaac tttgtagtta cggggcacga cttattcttg atttaaccgt ttactcgatt
     4141 ggagtatttg gaacggctta ttacttgata aatgatccca ataggattgc agcatcagac
     4201 atttttacag tgctaatgac gttgattttt gtcatgctat tgtctatttt tactcaatta
     4261 gtcatgcaat ttaccaggca aaaattaaca gcaggaaaac ttttcaaaag cctattcgat
     4321 ttaattgttg taaatgcaat tctagcgact ttaataaaac aaattgaaca atctggaaga
     4381 acaacaaaag gagtgaagga ttttttattg acgtttattc actttgtaaa tggacacatg
     4441 tatttgtttg aagtgggtat tattctcttt tttgtcgggt atgtatccac gaaacttatt
     4501 aaacgttcaa ttgaaaaaga aattgaaatt gacctgattc cgacacttct tggttatgag
     4561 tacaatgttg attttggaag atatgacgaa ttgatcaata cggatatcgt tcacacgagt
     4621 atccatgaat ctactgttaa gggcatcaga tctattttaa aaaatggcca ggaaatgaaa
     4681 gcaatagatc gaagaaaaga gatctacaat ttttatgaag tcgaagcaag cctacgatat
     4741 ggcatcggaa aattgtggtt agatggaagt aatattcgtc aaaacaaacc atacattttt
     4801 tggaacagta gtgatttaga acttccaatg aaagataaat aattatggag aatcgtttga
     4861 aaatgattcc tctatacagg attgtgctta tagttggcat ggagttaaaa accttaaaga
     4921 aggagcagtt attcaaagtg aaagaacgtc ataatcagtt actatgtagt aaaacgggta
     4981 cgatgaacat tgaataccga acagaatatt tagaattaac gaatcaaact gattttttgt
     5041 tgattcgaaa gcaacaagag caagaaaaaa attaatggct acttatagcc aattgattga
     5101 tgaatgggaa caattcaagg aatctaaaat tccagaacgt ctctaaaagc ctttatttgt
     5161 aaagagcata aggaaacgtg ggaacacttt ttaaaaaaag tgttccaata ggctgtgcct
     5221 attacgtttc tcaaaaataa atgtgggaac aaaatattta aaaataggat agtgaaagga
     5281 tagtgaagga tagtaaaagg agggtagtag gatagcaaaa aggataggct ccttttcgct
     5341 atccttttta aattggagga attaatatgg aagtaagaat aagaaatgtg gatgagaaaa
     5401 tcgtaagaga aattgatcga agatgtgacg aacggttctg ttgcaaagtt ttaaatctac
     5461 tatcaaataa ggtagaataa tagaaaaaga tagcaggagg aatgacgatg aatcatttta
     5521 aaggaaagca atttcagcag gatgtgatta ttgtagccgt gggctactat cttcgttata
     5581 accttagcta tcgtgaagtt caagaaatct tatatgatcg tggcattaac gtttctcata
     5641 cgacgattta tcgttgggtg caagaatatg gcaaactact ctatcaaatt tggaaaaaga
     5701 aaaataaaaa atccttttat tcatggaaaa tggatgaaac gtacatcaaa attaaaggaa
     5761 aatggcatta tttgtatcga gccatcgatg cagatggttt aaccttggat atttggttac
     5821 gtaaaaaacg ggacacacaa gcagcctatg cttttcttaa gcggttagtg aagcagtttg
     5881 atgaaccgaa ggttgtagtc acagataaag ccccctctat tacaagtgcc tttaagaaac
     5941 taaaagaata cggcttttat caagggacag aacatcgtac cattaaatac ctgaataatt
     6001 tgattgaaca agaccatcgt ccagtaaaga gacgcaataa attctatcga agtttacgca
     6061 ctgcctctac cacgattaaa ggcatggaag ccattcgagg attatataag aaaacccgaa
     6121 aagaaggcac tctcttcggg ttttcggtct gtactgaaat caaggtatta ttgggaatcc
     6181 cagcttaaat catagatacc gtaagggatt ttattcttta tttaaaactt tgcaacagaa
     6241 cccttttgtt taataacttc agcaacaacc ttttgtgact gaatgccatg tttcattgct
     6301 ctcctccagt tgcacattgg acaaagcctg gatttacaaa accacactcg atacaacttt
     6361 ctttcgcctg tttcacgatt ttgtttatac tctaatattt cagcacaatc ttttactctt
     6421 tcagcctttt taaattcaag aatatgcaga agttcaaagt aatcaacatt agcgattttc
     6481 ttttctctcc atggtctcac ttttccactt tttgtcttgt ccactaaaac ccttgatttt
     6541 tcatctgaat aaatgctact attaggacac ataatattaa aagaaacccc catctattta
     6601 gttatttgtt tggtcactta taactttaac agatggggtt tttctgtgca accaatttta
     6661 agggttttca atactttaaa acacatacat accaacactt caacgcacct ttcagcaact
     6721 aaaataaaaa tgacgttatt tctatatgta tcaagataag aaagaacaag ttcaaaacca
     6781 tcaaaaaaag acaccttttc aggtgctttt tttattttat aaactcattc cctgatctcg
     6841 acttcgttct ttttttacct ctcggttatg agttagttca aattcgttct ttttaggttc
     6901 taaatcgtgt ttttcttgga attgtgctgt tttatccttt accttgtcta caaacccctt
     6961 aaaaacgttt ttaaaggctt ttaagccgtc tgtacgttcc ttaaggaatt ccttagtgct
     7021 ttcatagatt aaactcacat cacgctttaa atcgcttatt ttagacttta aagacttgtt
     7081 ttcttcaagc aactcattat aatcatttac attttcatta aatcgctcta caagaccact
     7141 atatttttct ttaacttgcc catgttcttt acttaatttt ttatattctc tcgccatatc
     7201 agtactcatg agatttctaa catgctgttt taacctatcg ttatctctcg cagcagtcac
     7261 taagttttta taatcacgct ccgatataac aacatttttg gttggtttct tttctgtttt
     7321 cattatttct tttcccaaac caaacatgga cttttcaccc gttggcactt caacactttt
     7381 catgtgtcgt ttcgctggta cttctaaatc tgatttaact ttatcgctat aagcagtcca
     7441 ttcatctttt ttaactgcta aatttttttc tagaaaatca atctcttttt ccaaagtttg
     7501 ttttttaaat ttagctgtct caatatgttt acggtcagag ccacgttcac cacgcttcaa
     7561 ctcaaaaccc tgttttttca tatgctcggg gaatttatct tgtagccata acagttcttg
     7621 acgattaaac acattttttc cttgcagttt tccatcacgc ataggcacaa cacctaaatg
     7681 catgtgaggg gtttgctcat cattatgaac tgttgcataa gcaatatttt gcttgccata
     7741 tcgttcggaa aataatttat aactttcctc aaaaaatcgt ttttgttctc ctggatccag
     7801 ttgctcaaaa aaatctcggt cagatgttac tagcaactca tttacaagaa cagcatcttt
     7861 cctcgttttt cttgtacctg ttttttgtga ttcaataatt tctttgacac gttcgttgta
     7921 atcaatattt ttatcatttt tcaaatcata attttcacgt gttcgctcat ggtcaatatc
     7981 atcattcgtt ctactttttc gctctctttg attatgaaat tgcatgcctt ttagtccagc
     8041 tgatttcact ttttgcattc tacaaactgc ataactcata tgtaaatcgc tcctttttag
     8101 gtggcacaaa tgtgaggcat tttcgctctt tccggcaacc acttccaagt aaagtataac
     8161 acactatact ttatattcat aaagtgtgtg ctctgcgagg ctgtcggcag tgccgaccaa
     8221 aaccataaaa cctttaagac ctttcttttt tttacgagaa aaaagaaaca aaaaaacctg
     8281 ccctctgcca cctcagcaaa ggggggtttt gctctcgtgc tcgtttaaaa atcagcaagg
     8341 gacaggtagt attttttgag aagatcactc aaaaaatctc cacctttaaa cccttgccaa
     8401 tttttatttt gtccgttttg tctagcttac cgaaagccag actcagcaag aataaaattt
     8461 ttattgtctt tcggttttct agtgtaacgg acaaaaccac tcaaaataaa aaagatacaa
     8521 gagaggtctc tcgtatcttt tattcagcaa tcgcgccctt taatggaaca aaaagatttg
     8581 aattcctgtt ataaaaaaag gatcaatttt gaactctctc ccaaagttga tcccttaacg
     8641 atttagaaat ccctttgaga atgtttatat acattcaagg taaccagcca actaatgaca
     8701 atgattcctg aaaaaaggaa tagcatatta ctatacaagt aagtggattg atcaaggtcc
     8761 ataggtagca acttctgatt cagcaagcgt acagataata aaccaccaac aattgcaata
     8821 cctgttccct ctgataaaaa gctggtaaaa ttaagcaaac tcattccggc accagcttcc
     8881 ttttgtttca aactacttga aacaattgta gatataactg ttttggtgaa tgacagccca
     8941 ccaaaaacaa aaactaatat aattgtcagt aaccatggtg ttacttctaa aaaaaaggca
     9001 gcagttaaaa agctaacaga aagaaatgta actccgatgt ttaacacgta taaaggacct
     9061 cttctatcaa caagtatccc accaatgtag ccgaaaataa tgacactcat tgttccaggg
     9121 aaaataatta cacttccgat ttcggcagta cttagctggt gaacatcttt catcatataa
     9181 ggaaccatag agacaaaccc tgctactgtt ccaaatataa ttcccccaca aagaactcca
     9241 atcataaaag gtatattttt ccctaatccg ggatcaacaa aaggatctgt tactttcctg
     9301 atatgtttta caaatatcag gaatgacagc acgctaacga taagaaaaga aatgctatat
     9361 gatgttgtaa acaacataaa aaatacaatg cctacagaca ttagtataat tcctttgata
     9421 tcaaaatgac cttttatcct tacttctttc tttaataatt tcataagaaa cggaacagtg
     9481 ataattgtta tcataggaat gagtagaaga taggaccaat gaatataatg ggctatcatt
     9541 ccaccaatcg ctggaccgac tccttctccc atggctacta tcgatccaat aagaccaaat
     9601 gctttacccc tattttcctt tggaatatag cgcgcaacta caaccatcac gagtgctgga
     9661 aatgcagctg caccagctcc ttgaataaat cgagccagaa taagtatggg aaagaaagaa
     9721 tgtccaacaa acccaattac cgatccgaag caatttatta taatcccaaa taggagtaac
     9781 cttttaatgc ctagctgatc agatagcttt ccatatagag ctgttccaat agaaaaggtt
     9841 aacataaagg ctgtgttcac ccagtttgta ctcgccggtg gtttattaaa atcatttgca
     9901 atatcaggta atgagacgtt caaaaccatt tcatttaata cgctaaaaaa agataaaatg
     9961 caaagccaaa ttaaaatttg gttgtgtcgt aaattcgatt gtgaatagga tgtattcaca
    10021 tttcaccctc caataatgag ggcagacgta gtttataggg ttaatgatac gcttccctct
    10081 tttaattgaa ccctgttaca ttcattttac ttcataatta attcctcctt tacactttaa
    10141 ttcaaatctt tattaaaaaa tatttcatct cgtttcgttt aacaagaagc cacatttata
    10201 taacaatata aaatgtgcta agttatttta ttgaacatat atcgtacttt atctatccga
    10261 ctatttggac gacggggctg gcaaacaggt tcgccagtgg taacctgata tccttttaac
    10321 tctgttaaac aaacgctacg tccatttgta aagaaagtta aatcattacg atattcttga
    10381 atacaccgag caggaatttc tccactaaga atgacctcat tatttttcag ttgagtgtct
    10441 acgatgttcg cacaatattt aggagcatcg tcatatgctc gtgaaagata ttcttgtggt
    10501 gcataaattt taaaactaag atatggctct aacaattctg ttccagcttt ttttaaaact
    10561 tgttccaata caataggagc aagcatccga aaatctgctg gggtactaac agggctatag
    10621 tataagccat acttaaaaca gattttacaa tccgtcacat tccaaccata taatccttgt
    10681 tcgcaaccat agcgtatccc ttccataact gcattttgaa atgattgatt taagtatcca
    10741 agagaaaccg agctctcata ctgcattcca cttcccaacg gaagcggtga tacagataaa
    10801 ccaatggaag cccagaaggg attcggtggc acttcgatgt gaatggtata ctctgctttt
    10861 tttaacggtc tttccatata aatgactgta ggctttttta tttttacctc cacatgatac
    10921 ttttcttgca atagagcaca agtcacttcc atttgtactt tccctaagaa agaaagtata
    10981 atttcatgtg tcgtagaatc cacataatat tgtagaagcg ggtcactgtc ggagatttct
    11041 aaaagtgcat caagtaacat ttccctttgt tgaggtttgc tcggttcaac agttgtttgc
    11101 agcagaggga ggggattttc aattctctct ctctgtggca atagctttgt atctccaaga
    11161 acactattta acttcaaaaa ctcattctgc aaaataacaa tttccccgga ataagcctta
    11221 tcgattttac ataattcacc atttattgaa gtatacattt ctgtaatttt tattttttcc
    11281 ttttccgata ttctaaccga atctcgcaaa tgcagtacgc cactataaag acgtatatat
    11341 gcaagacgtt gtcttttttt tgtatattca attttgaaaa catttccgca aagttcagac
    11401 tgacctcgat gtgttgatga ataaaattta ttcgtaatca cttctataag gttatcaatc
    11461 cctatattgt tttttgcact tccgtgataa acagggaaca gggaacaatt ctgaaatctt
    11521 atgctttcct cttgttcgag ttccaatgct tctaatgatt taccggacat atatttctct
    11581 aaaaggtcat cgtttccctc tattaccgta tcccattgtt cagattcggt aaagttcgtc
    11641 acacacatat taggatacag ttctaccttc tgtttgatta caatttcggc agaaagtttc
    11701 tctttaatat cctgataaac cgttgataaa tcaattccat tttggtcaat cttattgata
    11761 aaaaagattg tgggaatccc cattttccta agtgcatgaa ataatatacg agtttgtgct
    11821 tgtacgccat cttttgcaga aatcagtaga attgccccat ctaaaactga taatgaacga
    11881 tatacttctg ctaagaaatc catatgtcct ggcgtgtcta tgatgttcac cttcgtattt
    11941 tcccactgaa aagaggttat tcctgtctga attgtaattc ctctctgacg ttctaaaagc
    12001 gtattatccg tcctcgttgt acctttgtcc acgcttccta attctgtaat cgctccactg
    12061 ttatataata agctttctgt taaggtagtt tttcccgcat caacatgagc taaaactcca
    12121 atattaataa ttttcatgtg attttcctcc attcaaaagc ccaaaagggc ataaaaatcc
    12181 cagtgataaa tactcttatc actgggattt ttatgcataa ccataggcat acaaagcata
    12241 cagatattct ccggatactt tagaatcaca tgataaaggt attcttaaac tgggtacaaa
    12301 aaactaagcc ctcctaaaaa aggacatcca attatttgtt cccactatca aattgacagt
    12361 ttatttaaga ataccttgcc gcatatttat taactccttt taaatagata cttaaataat
    12421 agcacgtaag agcatatttg taaaggaatc tccaattttt tatcaaagag agtacgtgat
    12481 tacaaaatag ctgtaataat gtaccaatat ttgttattct ataatcttcc aattactccc
    12541 gttcttttca agtaccaaat caaattgaga tacctgcgtt gctttggtct gctggtcgat
    12601 atactccact gtcagcgata ccgtgacttg attatcctta cgattgtgaa taggatttac
    12661 cagttcttga aagatgtact cttttccgat tggttttaat atcccgtcat tcacatagta
    12721 ggaaagttca ctggctgtcg ctgtaggata gagcttgaag aacgtcgtta aaaactcatt
    12781 gatttcattg gttgtaatgg aatcaaccgt cccctcactt tcaatggctt ttggtttata
    12841 acttgatttc ttaggtatgt tggtaatggt cggattctta accagtacca tatttccaga
    12901 accatctaca tagacactca ctatataagc agagtggacg gtctttgtat tttctccctc
    12961 tgtaatgagc tggtctacac tgtaggtta
//
