LOCUS       NZ_CP116531            18533 bp    DNA     circular CON 01-MAR-2024
DEFINITION  Enterococcus faecium strain K188-5 plasmid pK188-5-C, complete
            sequence.
ACCESSION   NZ_CP116531
VERSION     NZ_CP116531.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23424531
            Assembly: GCF_028335485.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18533)
  AUTHORS   Biggel,M.
  TITLE     Oxazolidinone resistance genes in florfenicol resistant enterococci
            from beef cattle and veal calves at slaughter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18533)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2023) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x, 50x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/01/2024 01:52:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,802
            CDSs (total)                      :: 2,712
            Genes (coding)                    :: 2,623
            CDSs (with protein)               :: 2,623
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 89
            CDSs (without protein)            :: 89
            Pseudo Genes (ambiguous residues) :: 0 of 89
            Pseudo Genes (frameshifted)       :: 35 of 89
            Pseudo Genes (incomplete)         :: 47 of 89
            Pseudo Genes (internal stop)      :: 24 of 89
            Pseudo Genes (multiple problems)  :: 14 of 89
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116531.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..18533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="K188-5"
                     /isolation_source="fecal sample"
                     /host="Bos taurus"
                     /db_xref="taxon:1352"
                     /plasmid="pK188-5-C"
                     /collection_date="2021"
                     /collected_by="ILS, University of Zurich"
     gene            join(18057..18533,1..96)
                     /locus_tag="PML77_RS13875"
                     /old_locus_tag="PML77_13875"
     CDS             join(18057..18533,1..96)
                     /locus_tag="PML77_RS13875"
                     /old_locus_tag="PML77_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000170424.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRDR
                     PKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTSMG
                     RMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELLTSG
                     KSYKEVEAITGFSRSTLFRIKKKIEES"
                     /name="recombinase family protein"
     gene            complement(529..1488)
                     /locus_tag="PML77_RS13880"
                     /old_locus_tag="PML77_13880"
     CDS             complement(529..1488)
                     /locus_tag="PML77_RS13880"
                     /old_locus_tag="PML77_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1252 family transposase"
                     /protein_id="WP_000222577.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKIVKIAEYLKRSRTPIYNVINFL
                     KEGHTALEYYQQYKENKKRCGRRNIVLPKKQQSYIKEKIAQGWTPDVIIGRKEKPISCS
                     VRTLYRQFKEKVFDESTLPMKGKRKPNGHQERRGKQAFKRNIVERETDYPTFKEEFGHI
                     EGDTIVGIHHKSAVITLVERLSKIIITLKPEGRKAIDIENTLNHWFGSIPRHLFKSITF
                     DCGKEFSNWKALSNQHDIAIYFADPGTPSQRALNENSNGLLRKDGLPKNMDFNQVDQAF
                     ISSVANKRNNIPRKSLDYQTPLEVFMSYMNEDILSSLI"
                     /name="IS30-like element IS1252 family transposase"
     gene            complement(1616..2296)
                     /locus_tag="PML77_RS13885"
                     /old_locus_tag="PML77_13885"
     CDS             complement(1616..2296)
                     /locus_tag="PML77_RS13885"
                     /old_locus_tag="PML77_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            complement(2524..2799)
                     /gene="prgO"
                     /locus_tag="PML77_RS13890"
                     /old_locus_tag="PML77_13890"
     CDS             complement(2524..2799)
                     /gene="prgO"
                     /locus_tag="PML77_RS13890"
                     /old_locus_tag="PML77_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="WP_001196543.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVDP
                     PVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
                     /name="prgO"
     gene            complement(2771..3724)
                     /locus_tag="PML77_RS13895"
                     /old_locus_tag="PML77_13895"
     CDS             complement(2771..3724)
                     /locus_tag="PML77_RS13895"
                     /old_locus_tag="PML77_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_000429439.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLFE
                     KDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGSPG
                     SEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAADYII
                     AMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQKTID
                     KYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYYEQHGD
                     IEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
                     /name="ParA family protein"
     gene            4336..5829
                     /locus_tag="PML77_RS13900"
                     /old_locus_tag="PML77_13900"
     CDS             4336..5829
                     /locus_tag="PML77_RS13900"
                     /old_locus_tag="PML77_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="WP_000947691.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSKQ
                     LMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINTFV
                     VDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVIDVA
                     KKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSMKLES
                     DQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTLSLAYY
                     SSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELCQQWVSP
                     TLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLNLSKKELA
                     SQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLSKEKRLDYS
                     TRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
                     /name="primase C-terminal domain-containing protein"
     gene            5964..6260
                     /locus_tag="PML77_RS13905"
                     /old_locus_tag="PML77_13905"
     CDS             5964..6260
                     /locus_tag="PML77_RS13905"
                     /old_locus_tag="PML77_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication control protein PrgN"
                     /protein_id="WP_000053907.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTRE
                     KTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
                     /name="replication control protein PrgN"
     gene            6361..7212
                     /locus_tag="PML77_RS13910"
                     /old_locus_tag="PML77_13910"
     CDS             6361..7212
                     /locus_tag="PML77_RS13910"
                     /old_locus_tag="PML77_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002287227.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILDL
                     TVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAGKL
                     FKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILFFVG
                     YVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKGIRSI
                     LKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDLELPMK
                     DK"
                     /name="hypothetical protein"
     gene            7233..7445
                     /locus_tag="PML77_RS13915"
                     /old_locus_tag="PML77_13915"
     CDS             7233..7445
                     /locus_tag="PML77_RS13915"
                     /old_locus_tag="PML77_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002324171.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIEY
                     RTEYLELTNQTDFLLIRKQQEQEKN"
                     /name="hypothetical protein"
     gene            7878..8558
                     /locus_tag="PML77_RS13920"
                     /old_locus_tag="PML77_13920"
     CDS             7878..8558
                     /locus_tag="PML77_RS13920"
                     /old_locus_tag="PML77_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            complement(<8604..8942)
                     /locus_tag="PML77_RS13925"
                     /old_locus_tag="PML77_13925"
                     /pseudo=""
     CDS             complement(<8604..8942)
                     /locus_tag="PML77_RS13925"
                     /old_locus_tag="PML77_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            complement(9187..10449)
                     /gene="mobV"
                     /locus_tag="PML77_RS13930"
                     /old_locus_tag="PML77_13930"
     CDS             complement(9187..10449)
                     /gene="mobV"
                     /locus_tag="PML77_RS13930"
                     /old_locus_tag="PML77_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000119405.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYD
                     LKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFE
                     ESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKF
                     PEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVK
                     SDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDND
                     RLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSK
                     ISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNE
                     FELTHNREVKKERSRDQGMSL"
                     /name="mobV"
     gene            complement(11013..12389)
                     /gene="tet(L)"
                     /locus_tag="PML77_RS13935"
                     /old_locus_tag="PML77_13935"
     ARG             complement(11017..12389)
                     /name="tet(L)"
                     /gene="tet(L)"
                     /gene_name="tetracycline efflux MFS transporter Tet(L)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="95.41"
                     /coverage="100.0"
     gene            complement(12588..14507)
                     /gene="tet(M)"
                     /locus_tag="PML77_RS13940"
                     /old_locus_tag="PML77_13940"
     ARG             complement(12592..14507)
                     /name="tet(M)"
                     /gene="tet(M)"
                     /gene_name="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="98.12"
                     /coverage="100.0"
     gene            complement(14523..14639)
                     /locus_tag="PML77_RS13945"
                     /old_locus_tag="PML77_13945"
     CDS             complement(14523..14639)
                     /locus_tag="PML77_RS13945"
                     /old_locus_tag="PML77_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="WP_001814923.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
                     /name="tetracycline resistance determinant leader peptide"
     gene            complement(14884..15816)
                     /locus_tag="PML77_RS13950"
                     /old_locus_tag="PML77_13950"
     CDS             complement(14884..15816)
                     /locus_tag="PML77_RS13950"
                     /old_locus_tag="PML77_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="WP_001224318.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVLL
                     GLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSIDN
                     RMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQLITE
                     GENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSITTNEI
                     NEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTVSLTVE
                     YIDQQTKATQVSQFDLVLEKNGSNWKIIE"
                     /name="conjugal transfer protein"
     gene            complement(15813..>16526)
                     /locus_tag="PML77_RS13955"
                     /old_locus_tag="PML77_13955"
                     /pseudo=""
     CDS             complement(15813..>16526)
                     /locus_tag="PML77_RS13955"
                     /old_locus_tag="PML77_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /name="C40 family peptidase"
     gene            16558..17238
                     /locus_tag="PML77_RS13960"
                     /old_locus_tag="PML77_13960"
     CDS             16558..17238
                     /locus_tag="PML77_RS13960"
                     /old_locus_tag="PML77_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="WP_001015311.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6-like element IS1216 family transposase"
     gene            17436..18041
                     /locus_tag="PML77_RS13965"
                     /old_locus_tag="PML77_13965"
     CDS             17436..18041
                     /locus_tag="PML77_RS13965"
                     /old_locus_tag="PML77_13965"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="WP_000599739.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTFK
                     GLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEIIKK
                     YVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDLEIR
                     YLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
                     /name="protein adenylyltransferase Fic"
     ARG             complement(11014..12299)
                     /name="tet(45)"
                     /gene="tet(45)"
                     /gene_name="major facilitator superfamily (MFS) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="77.75"
                     /coverage="93.45"
     Typing          4337..5829
                     /name="rep_cluster_185"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(9191..10449)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.19"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cttacttcag gtaaatcgta taaggaagtt gaagctataa ccggtttcag tcgcagtacg
       61 ctttttagaa taaagaagaa aattgaagaa agttaaaaaa gaagtctcag cgaaccagac
      121 aggtaaatac ttaatgcgtt ctagatatga gtctattagc atgttgtgaa atataatatt
      181 tttttattta cttgatttct gctcaaaaaa tgtgtatggt aactgtaaaa ggtaaagtaa
      241 ttaaaggtat gctagccacg cttactatat taggatacca tcatatattt ataatgaacg
      301 atttgttcgt ctaccaaaca acacatatcg ttcagcgtaa gtcacgttta ggaacgtggc
      361 ttttttagtg ttattatcga taaataatct attgactcta ttgattttat ctccatatcc
      421 ctacggtgtt gattgtcttt agatctttca tttaagaacc aaataactat cataaaaatc
      481 agcactgctg ctaaggttct gttgcaaagt tttaaataat ttgatttgtc aaattaagct
      541 agacaaaata tcttcattca tgtaactcat aaatacttcc aggggtgttt gataatccaa
      601 ggactttcgt ggaatattgt ttctcttatt cgccactgaa gagataaaag cttggtcgac
      661 ttgattaaaa tccatgtttt ttggcagacc atcttttcgc agcaagccat tggaattttc
      721 attcagtgct cgttgcgaag gagtccctgg atcagcgaaa taaatagcga tgtcatgttg
      781 gttacttaaa gccttccaat tagagaattc tttcccacaa tcaaaggtaa tggatttaaa
      841 taaatgtctg ggaatcgatc caaaccagtg gttcagagta ttctcaatat cgatcgcctt
      901 gcgcccctcg ggcttcaagg tgatgatgat tttcgataga cgttctacca aagttatgac
      961 agcactctta tgatgaatgc ctacaatggt atcgccttcg atatggccga actcttcttt
     1021 aaatgtcggg taatcagttt ctctttcaac gatattccgt ttaaaagcct gttttcctcg
     1081 gcgttcttga tgtccatttg gtttccgctt acccttcatg ggcaaagtcg attcatcgaa
     1141 caccttttcc ttgaactggc gataaagggt tcttacggaa cagctgattg gtttctcttt
     1201 acgcccaatg ataacgtctg gcgtccagcc ttgggcaatt ttttctttaa tgtaggactg
     1261 ttgtttcttt ggcaagacga tgtttcgtct gccacaccgt ttcttatttt ctttatactg
     1321 ttggtagtat tcgagggccg tatggccttc tttcaagaaa ttaatgacgt tataaatcgg
     1381 tgttcgagat cgtttcaggt actcagcaat tttcacaatt ttgatgtttt ggtggtagta
     1441 agactctatc atcaccagtt catccgtggt aagatgggtg taggtcattc gtgatcactc
     1501 ctatgttttc tttagtcgga attacatttt gagtgtagca cgaatggctt atttatttgt
     1561 ctagcttaat tttacaatcg gcgtaaagaa taaaatccct tacggtatct atgatttaag
     1621 ctgggattcc caataatacc ttgatttcag tacagaccga aaacccgaag agagtgcctt
     1681 cttttcgggt tttcttatat aatcctcgaa tggcttccat gcctttaatc gtggtagagg
     1741 cagtgcgtaa acttcgatag aatttattgc gtctctttac tggacgatgg tcttgttcaa
     1801 tcaaattatt caggtattta atggtacgat gttctgtccc ttgataaaag ccgtattctt
     1861 ttagtttctt aaaggcactt gtaatagagg gggctttatc tgtgactaca accttcggtt
     1921 catcaaactg cttcactaac cgcttaagaa aagcataggc tgcttgtgtg tcccgttttt
     1981 tacgtaacca aatatccaag gttaaaccat ctgcatcgat ggctcgatac aaataatgcc
     2041 attttccttt aattttgatg tacgtttcat ccattttcca tgaataaaag gattttttat
     2101 ttttcttttt ccaaatttga tagagtagtt tgccatattc ttgcacccaa cgataaatcg
     2161 tcgtatgaga aacgttaatg ccacgatcat ataagatttc ttgaacttca cgatagctaa
     2221 ggttataacg aagatagtag cccacggcta caataatcac atcctgctga aattgctttc
     2281 ctttaaaatg attcatcgtc attcctcctg ctatcttttt ctattattct accttatttg
     2341 atagtagatt taaaactttg caacagaacc atatttagta tagtaaaaac tagaaacaat
     2401 aaaatttgtg aatgttgtat acccccaaac aatacacata ttttgaagga ttaggattaa
     2461 taaagctaaa aatatagtaa gtatactaaa tatatttact atatctagta tacttactat
     2521 attttaatct tttgatcgtc tattatatat aatccgttcc tcgtcgttta aagtatcaac
     2581 ataggcgtcc attgcaagtt tgactatttc atacatagga atatcttttg cataactaag
     2641 atcccgaatg gtttttagta cgggaggatc tacttgaact ggttttcttt ctttcgcagt
     2701 gtactttcgt tcccctaact cgacattaat atcatcttta attgaaatat cttcagcacc
     2761 aattttcttc ttatctttgt tcgttggtaa taatgccatt tagtttaatc tcctttccaa
     2821 gaggtaggat atgattggcc attgagtcag catatttagg tgtataatca aatccttcaa
     2881 tatcaccatg ttgttcatag tattcaattc tttcttctaa ttctgtaaag atgtctgcaa
     2941 aaatagccca ccatcttcga tctacataat catttaattg gataccattc tcaccataca
     3001 tttctaaccg atccgagcct ttaacaatgc ttttaaaaat gtttgattca ccatacttat
     3061 caattgtttt ttgatagttt gcgtgttgag aggaacgtcg cactgagaaa agaacaggca
     3121 aaattccaat gatttgaaga tgagactctt caaaattcgt gacaatcgga actaaaactt
     3181 tattaataaa atcttctgtt ccttccattg cgtaccgttt taattcttgc attgcaataa
     3241 tataatctgc agcagttaag aatgcacgaa ccacattatc tgttgatggt gggcagtcga
     3301 taaagatata atcgaaattt tttctcaatt cttctaaagg tttaatgaag gccaatgatc
     3361 tcttacgacg atcaggatat ttttcggaca agtattcatt aagttcttca gatccaggag
     3421 agccagccat aatatataaa tgtggactta cttgagtaat acctgaaacc cagtcccctt
     3481 ttttgattgc tgcagacatg gatcgatcaa agctagtaat ttgatacgtt gatccaatgt
     3541 tggaagtagt atttctttgt tggtcataat caattagcaa cacgtctttt tcaaaaagtt
     3601 gactagaaac aatcgctaat aaatcattgt ctgtagtctt accaatgccg ccttttctac
     3661 ctgtaagcat tacaactttt cctagtttat ctgatgtttt aaatgcagtc cgtaagtttt
     3721 ccatagttcc ctccaatttc ctatactaaa tatatttagt atacttcata tacttaatat
     3781 attcgatata tctaatatac tccaaattga taagaattca gactttataa ggaatatagt
     3841 ggatatacta aatatatttg ctatattcag tatatcagat atattcaaaa tgtcaaaata
     3901 tatttgtgcg ttttgcgttg acaatttaga gtgaaaagag gtaaacttaa ttctatagaa
     3961 accacagata attcactatt tacaaagtag caaaagcagt ggtactcttt aaacagaaaa
     4021 attacataaa aaaaagagac ggaaataatt gattttagca gaatcaatta tttctgaatc
     4081 acatggttag gacatgtgtt cacgctcgtc tcaagaatat gtaagtagct accaactact
     4141 tatattaaaa gttgatcgga taccttttcc gattgccttt attctaccaa aaaaaggcga
     4201 gcaactcaac agaagaaagt ttatttagtg tacttaaatt aaatattctt aaacactgaa
     4261 gagagaacac atactatggc tgagattaaa gctaaactgt gttctctttt tgtttgagaa
     4321 aggacgttac aatcgatgtt gagcgaaatt tattcagcta ttcttaaaaa cggcttacgt
     4381 acatacaaat atcgaaacag caccatgaaa cctgcaggtc atattgattc cagtaggagt
     4441 ggtgctatct ttgcgtaccg tagcaagcaa ttaatgaatg ttgggcgtgg tatggtcatt
     4501 acttcggaag aagctatttt agagaacgaa aagaaactaa cgcattggac acctaatacc
     4561 tatcgctttg gtacttatgc ggatgattat cgaacagtgg ttaaaggtca ttctgagaaa
     4621 aacttaagtc aaatcaacac atttgtagtt gatatcgatt caaaagagaa ccatcaaggc
     4681 gaaattattt tagcctgttt agatcaagtg ggctacatgc caacgctaat tttagaatca
     4741 gatcatggtt atcaagttta ttttgtacta aagacaccag gctatgtcac aaagaaaagc
     4801 aatttcaagg tgatcgatgt agcaaaaaaa atttcaaaaa caattcgtct acagttggct
     4861 gaaaaattgt ccggagtgga tctaggatgt aaccacttcg gaattgctcg attcccacaa
     4921 gaaaataata ttgtttttaa agaaatgaac tatcagtatt cttttgctga ctggattcaa
     4981 tggtcgatga agcttgaatc agatcaaaaa acaaaagaag atcgagaacg aaaactaatt
     5041 gtctttcctg agaaaaaaga aattcgtcaa gtagatgaac cgtggttcga tatgttactt
     5101 cataaagcaa acatcgtcgg cggagaagga cgtcttggtc gaaataatgt gattttcacg
     5161 ctttctttag cttattactc ttcaggttat gcattggata cttgcgaata caatatgttc
     5221 acattcaacg aacggttaca ggaaccgcta tctgaagcag aattagtaaa gattgtacgt
     5281 agtgcttatt ctggtaatta tcaagcagca agcagagaat tcattattga attgtgccag
     5341 caatgggttt caccaacctt aaaaaataat gatctgttca ttcaacgacg tggctggtgg
     5401 aaatttaaaa agccacgaga tcaaagaaaa tatagtcata aaaatgaatg gaaagaagat
     5461 ctattggctt atctaagcga aaaaagtctt gctagtgagc cttatttgaa cctgtcaaaa
     5521 aaagaattgg cttcacaact aacgatgccc aagcgaacac ttgataagat cttaaatgag
     5581 ttacgagagg agaataaagt cttctacaag gtaacacgag gaagaaatgg cggaatgatc
     5641 attgcttcag tcaaaacgtt gttcgcgagg gttattcaac taagtaaaga aaagagacta
     5701 gattattcaa caaggatcac agaggtattt ggactcaacg agagcttagt agaagagaca
     5761 tttagacaat tatcaacccc cttgaaccct cttaaagaag tgaatctttt tgaagtagat
     5821 acaggttgaa aaataaaagg tgcaaattgc cctatacaat atatctatga tacttctgta
     5881 ggtgttgctc cctttgccta gctacgtgta ggtggtatac ttataactgc gtatgcagtc
     5941 ttatgtatca tggaggaagt tgtatgtcgt taaaaaatta tgtctattca catcctgtta
     6001 atgtgtttat cattggtcgt ttgggattat ctgtggaagt gttctgtgag ctttatggtt
     6061 ttaagcaggg gactttgtct tcttgggtta ccagggaaaa aacagttgct tctttgccga
     6121 ttgagtttct tcatgggttg tccttagctt ctggggagac tatggatcag gtatatgatt
     6181 gtttgtgtgt gttagagcag gagtatattg agtataaaat agctaacgag ttaagaaaaa
     6241 ggaagaagta tatccagtag tagtttttat ttacttaaaa aattggttca ttgagcaacc
     6301 tatcgctgct aaagtagcaa aggaaaacgt taaaggaaag aatttaggga agaaatcgaa
     6361 atgaaatcgg acgactggat tgaacacacc tggcttcctc tgaaaagaaa tagcaaacga
     6421 aagaaatttt gggtattgca attatggcca ttaggaaaac tttgtagtta cggggcacga
     6481 cttattcttg atttaaccgt ttactcgatt ggagtatttg gaacggctta ttacttgata
     6541 aatgatccca ataggattgc agcatcagac atttttacag tgctaatgac gttgattttt
     6601 gtcatgctat tgtctatttt tactcaatta gtcatgcaat ttaccaggca aaaattaaca
     6661 gcaggaaaac ttttcaaaag cctattcgat ttaattgttg taaatgcaat tctagcgact
     6721 ttaataaaac aaattgaaca atctggaaga acaacaaaag gagtgaagga ttttttattg
     6781 acgtttattc actttgtaaa tggacacatg tatttgtttg aagtgggtat tattctcttt
     6841 tttgtcgggt atgtatccac gaaacttatt aaacgttcaa ttgaaaaaga aattgaaatt
     6901 gacctgattc cgacacttct tggttatgag tacaatgttg attttggaag atatgacgaa
     6961 ttgatcaata cggatatcgt tcacacgagt atccatgaat ctactgttaa gggcatcaga
     7021 tctattttaa aaaatggcca ggaaatgaaa gcaatagatc gaagaaaaga gatctacaat
     7081 ttttatgaag tcgaagcaag cctacgatat ggcatcggaa aattgtggtt agatggaagt
     7141 aatattcgtc aaaacaaacc atacattttt tggaacagta gtgatttaga acttccaatg
     7201 aaagataaat aattatggag aatcgtttga aaatgattcc tctatacagg attgtgctta
     7261 tagttggcat ggagttaaaa accttaaaga aggagcagtt attcaaagtg aaagaacgtc
     7321 ataatcagtt actatgtagt aaaacgggta cgatgaacat tgaataccga acagaatatt
     7381 tagaattaac gaatcaaact gattttttgt tgattcgaaa gcaacaagag caagaaaaaa
     7441 attaatggct acttatagcc aattgattga tgaatgggaa caattcaagg aatctaaaat
     7501 tccagaacgt ctctaaaagc ctttatttgt aaagagcata aggaaacgtg ggaacacttt
     7561 ttaaaaaaag tgttccaata ggctgtgcct attacgtttc tcaaaaataa atgtgggaac
     7621 aaaatattta aaaataggat agtgaaagga tagtgaagga tagtaaaagg agggtagtag
     7681 gatagcaaaa aggataggct ccttttcgct atccttttta aattggagga attaatatgg
     7741 aagtaagaat aagaaatgtg gatgagaaaa tcgtaagaga aattgatcga agatgtgacg
     7801 aacggttctg ttgcaaagtt ttaaatctac tatcaaataa ggtagaataa tagaaaaaga
     7861 tagcaggagg aatgacgatg aatcatttta aaggaaagca atttcagcag gatgtgatta
     7921 ttgtagccgt gggctactat cttcgttata accttagcta tcgtgaagtt caagaaatct
     7981 tatatgatcg tggcattaac gtttctcata cgacgattta tcgttgggtg caagaatatg
     8041 gcaaactact ctatcaaatt tggaaaaaga aaaataaaaa atccttttat tcatggaaaa
     8101 tggatgaaac gtacatcaaa attaaaggaa aatggcatta tttgtatcga gccatcgatg
     8161 cagatggttt aaccttggat atttggttac gtaaaaaacg ggacacacaa gcagcctatg
     8221 cttttcttaa gcggttagtg aagcagtttg atgaaccgaa ggttgtagtc acagataaag
     8281 ccccctctat tacaagtgcc tttaagaaac taaaagaata cggcttttat caagggacag
     8341 aacatcgtac cattaaatac ctgaataatt tgattgaaca agaccatcgt ccagtaaaga
     8401 gacgcaataa attctatcga agtttacgca ctgcctctac cacgattaaa ggcatggaag
     8461 ccattcgagg attatataag aaaacccgaa aagaaggcac tctcttcggg ttttcggtct
     8521 gtactgaaat caaggtatta ttgggaatcc cagcttaaat catagatacc gtaagggatt
     8581 ttattcttta tttaaaactt tgcaacagaa cccttttgtt taataacttc agcaacaacc
     8641 ttttgtgact gaatgccatg tttcattgct ctcctccagt tgcacattgg acaaagcctg
     8701 gatttacaaa accacactcg atacaacttt ctttcgcctg tttcacgatt ttgtttatac
     8761 tctaatattt cagcacaatc ttttactctt tcagcctttt taaattcaag aatatgcaga
     8821 agttcaaagt aatcaacatt agcgattttc ttttctctcc atggtctcac ttttccactt
     8881 tttgtcttgt ccactaaaac ccttgatttt tcatctgaat aaatgctact attaggacac
     8941 ataatattaa aagaaacccc catctattta gttatttgtt tggtcactta taactttaac
     9001 agatggggtt tttctgtgca accaatttta agggttttca atactttaaa acacatacat
     9061 accaacactt caacgcacct ttcagcaact aaaataaaaa tgacgttatt tctatatgta
     9121 tcaagataag aaagaacaag ttcaaaacca tcaaaaaaag acaccttttc aggtgctttt
     9181 tttattttat aaactcattc cctgatctcg acttcgttct ttttttacct ctcggttatg
     9241 agttagttca aattcgttct ttttaggttc taaatcgtgt ttttcttgga attgtgctgt
     9301 tttatccttt accttgtcta caaacccctt aaaaacgttt ttaaaggctt ttaagccgtc
     9361 tgtacgttcc ttaaggaatt ccttagtgct ttcatagatt aaactcacat cacgctttaa
     9421 atcgcttatt ttagacttta aagacttgtt ttcttcaagc aactcattat aatcatttac
     9481 attttcatta aatcgctcta caagaccact atatttttct ttaacttgcc catgttcttt
     9541 acttaatttt ttatattctc tcgccatatc agtactcatg agatttctaa catgctgttt
     9601 taacctatcg ttatctctcg cagcagtcac taagttttta taatcacgct ccgatataac
     9661 aacatttttg gttggtttct tttctgtttt cattatttct tttcccaaac caaacatgga
     9721 cttttcaccc gttggcactt caacactttt catgtgtcgt ttcgctggta cttctaaatc
     9781 tgatttaact ttatcgctat aagcagtcca ttcatctttt ttaactgcta aatttttttc
     9841 tagaaaatca atctcttttt ccaaagtttg ttttttaaat ttagctgtct caatatgttt
     9901 acggtcagag ccacgttcac cacgcttcaa ctcaaaaccc tgttttttca tatgctcggg
     9961 gaatttatct tgtagccata acagttcttg acgattaaac acattttttc cttgcagttt
    10021 tccatcacgc ataggcacaa cacctaaatg catgtgaggg gtttgctcat cattatgaac
    10081 tgttgcataa gcaatatttt gcttgccata tcgttcggaa aataatttat aactttcctc
    10141 aaaaaatcgt ttttgttctc ctggatccag ttgctcaaaa aaatctcggt cagatgttac
    10201 tagcaactca tttacaagaa cagcatcttt cctcgttttt cttgtacctg ttttttgtga
    10261 ttcaataatt tctttgacac gttcgttgta atcaatattt ttatcatttt tcaaatcata
    10321 attttcacgt gttcgctcat ggtcaatatc atcattcgtt ctactttttc gctctctttg
    10381 attatgaaat tgcatgcctt ttagtccagc tgatttcact ttttgcattc tacaaactgc
    10441 ataactcata tgtaaatcgc tcctttttag gtggcacaaa tgtgaggcat tttcgctctt
    10501 tccggcaacc acttccaagt aaagtataac acactatact ttatattcat aaagtgtgtg
    10561 ctctgcgagg ctgtcggcag tgccgaccaa aaccataaaa cctttaagac ctttcttttt
    10621 tttacgagaa aaaagaaaca aaaaaacctg ccctctgcca cctcagcaaa ggggggtttt
    10681 gctctcgtgc tcgtttaaaa atcagcaagg gacaggtagt attttttgag aagatcactc
    10741 aaaaaatctc cacctttaaa cccttgccaa tttttatttt gtccgttttg tctagcttac
    10801 cgaaagccag actcagcaag aataaaattt ttattgtctt tcggttttct agtgtaacgg
    10861 acaaaaccac tcaaaataaa aaagatacaa gagaggtctc tcgtatcttt tattcagcaa
    10921 tcgcgccctt taatggaaca aaaagatttg aattcctgtt ataaaaaaag gatcaatttt
    10981 gaactctctc ccaaagttga tcccttaacg atttagaaat ccctttgaga atgtttatat
    11041 acattcaagg taaccagcca actaatgaca atgattcctg aaaaaaggaa tagcatatta
    11101 ctatacaagt aagtggattg atcaaggtcc ataggtagca acttctgatt cagcaagcgt
    11161 acagataata aaccaccaac aattgcaata cctgttccct ctgataaaaa gctggtaaaa
    11221 ttaagcaaac tcattccggc accagcttcc ttttgtttca aactacttga aacaattgta
    11281 gatataactg ttttggtgaa tgacagccca ccaaaaacaa aaactaatat aattgtcagt
    11341 aaccatggtg ttacttctaa aaaaaaggca gcagttaaaa agctaacaga aagaaatgta
    11401 actccgatgt ttaacacgta taaaggacct cttctatcaa caagtatccc accaatgtag
    11461 ccgaaaataa tgacactcat tgttccaggg aaaataatta cacttccgat ttcggcagta
    11521 cttagctggt gaacatcttt catcatataa ggaaccatag agacaaaccc tgctactgtt
    11581 ccaaatataa ttcccccaca aagaactcca atcataaaag gtatattttt ccctaatccg
    11641 ggatcaacaa aaggatctgt tactttcctg atatgtttta caaatatcag gaatgacagc
    11701 acgctaacga taagaaaaga aatgctatat gatgttgtaa acaacataaa aaatacaatg
    11761 cctacagaca ttagtataat tcctttgata tcaaaatgac cttttatcct tacttctttc
    11821 tttaataatt tcataagaaa cggaacagtg ataattgtta tcataggaat gagtagaaga
    11881 taggaccaat gaatataatg ggctatcatt ccaccaatcg ctggaccgac tccttctccc
    11941 atggctacta tcgatccaat aagaccaaat gctttacccc tattttcctt tggaatatag
    12001 cgcgcaacta caaccatcac gagtgctgga aatgcagctg caccagctcc ttgaataaat
    12061 cgagccagaa taagtatggg aaagaaagaa tgtccaacaa acccaattac cgatccgaag
    12121 caatttatta taatcccaaa taggagtaac cttttaatgc ctagctgatc agatagcttt
    12181 ccatatagag ctgttccaat agaaaaggtt aacataaagg ctgtgttcac ccagtttgta
    12241 ctcgccggtg gtttattaaa atcatttgca atatcaggta atgagacgtt caaaaccatt
    12301 tcatttaata cgctaaaaaa agataaaatg caaagccaaa ttaaaatttg gttgtgtcgt
    12361 aaattcgatt gtgaatagga tgtattcaca tttcaccctc caataatgag ggcagacgta
    12421 gtttataggg ttaatgatac gcttccctct tttaattgaa ccctgttaca ttcattttac
    12481 ttcataatta attcctcctt tacactttaa ttcaaatctt tattaaaaaa tatttcatct
    12541 cgtttcgttt aacaagaagc cacatttata taacaatata aaatgtgcta agttatttta
    12601 ttgaacatat atcgtacttt atctatccga ctatttggac gacggggctg gcaaacaggt
    12661 tcgccagtgg taacctgata tccttttaac tctgttaaac aaacgctacg tccatttgta
    12721 aagaaagtta aatcattacg atattcttga atacaccgag caggaatttc tccactaaga
    12781 atgacctcat tatttttcag ttgagtgtct acgatgttcg cacaatattt aggagcatcg
    12841 tcatatgctc gtgaaagata ttcttgtggt gcataaattt taaaactaag atatggctct
    12901 aacaattctg ttccagcttt ttttaaaact tgttccaata caataggagc aagcatccga
    12961 aaatctgctg gggtactaac agggctatag tataagccat acttaaaaca gattttacaa
    13021 tccgtcacat tccaaccata taatccttgt tcgcaaccat agcgtatccc ttccataact
    13081 gcattttgaa atgattgatt taagtatcca agagaaaccg agctctcata ctgcattcca
    13141 cttcccaacg gaagcggtga tacagataaa ccaatggaag cccagaaggg attcggtggc
    13201 acttcgatgt gaatggtata ctctgctttt tttaacggtc tttccatata aatgactgta
    13261 ggctttttta tttttacctc cacatgatac ttttcttgca atagagcaca agtcacttcc
    13321 atttgtactt tccctaagaa agaaagtata atttcatgtg tcgtagaatc cacataatat
    13381 tgtagaagcg ggtcactgtc ggagatttct aaaagtgcat caagtaacat ttccctttgt
    13441 tgaggtttgc tcggttcaac agttgtttgc agcagaggga ggggattttc aattctctct
    13501 ctctgtggca atagctttgt atctccaaga acactattta acttcaaaaa ctcattctgc
    13561 aaaataacaa tttccccgga ataagcctta tcgattttac ataattcacc atttattgaa
    13621 gtatacattt ctgtaatttt tattttttcc ttttccgata ttctaaccga atctcgcaaa
    13681 tgcagtacgc cactataaag acgtatatat gcaagacgtt gtcttttttt tgtatattca
    13741 attttgaaaa catttccgca aagttcagac tgacctcgat gtgttgatga ataaaattta
    13801 ttcgtaatca cttctataag gttatcaatc cctatattgt tttttgcact tccgtgataa
    13861 acagggaaca gggaacaatt ctgaaatctt atgctttcct cttgttcgag ttccaatgct
    13921 tctaatgatt taccggacat atatttctct aaaaggtcat cgtttccctc tattaccgta
    13981 tcccattgtt cagattcggt aaagttcgtc acacacatat taggatacag ttctaccttc
    14041 tgtttgatta caatttcggc agaaagtttc tctttaatat cctgataaac cgttgataaa
    14101 tcaattccat tttggtcaat cttattgata aaaaagattg tgggaatccc cattttccta
    14161 agtgcatgaa ataatatacg agtttgtgct tgtacgccat cttttgcaga aatcagtaga
    14221 attgccccat ctaaaactga taatgaacga tatacttctg ctaagaaatc catatgtcct
    14281 ggcgtgtcta tgatgttcac cttcgtattt tcccactgaa aagaggttat tcctgtctga
    14341 attgtaattc ctctctgacg ttctaaaagc gtattatccg tcctcgttgt acctttgtcc
    14401 acgcttccta attctgtaat cgctccactg ttatataata agctttctgt taaggtagtt
    14461 tttcccgcat caacatgagc taaaactcca atattaataa ttttcatgtg attttcctcc
    14521 attcaaaagc ccaaaagggc ataaaaatcc cagtgataaa tactcttatc actgggattt
    14581 ttatgcataa ccataggcat acaaagcata cagatattct ccggatactt tagaatcaca
    14641 tgataaaggt attcttaaac tgggtacaaa aaactaagcc ctcctaaaaa aggacatcca
    14701 attatttgtt cccactatca aattgacagt ttatttaaga ataccttgcc gcatatttat
    14761 taactccttt taaatagata cttaaataat agcacgtaag agcatatttg taaaggaatc
    14821 tccaattttt tatcaaagag agtacgtgat tacaaaatag ctgtaataat gtaccaatat
    14881 ttgttattct ataatcttcc aattactccc gttcttttca agtaccaaat caaattgaga
    14941 tacctgcgtt gctttggtct gctggtcgat atactccact gtcagcgata ccgtgacttg
    15001 attatcctta cgattgtgaa taggatttac cagttcttga aagatgtact cttttccgat
    15061 tggttttaat atcccgtcat tcacatagta ggaaagttca ctggctgtcg ctgtaggata
    15121 gagcttgaag aacgtcgtta aaaactcatt gatttcattg gttgtaatgg aatcaaccgt
    15181 cccctcactt tcaatggctt ttggtttata acttgatttc ttaggtatgt tggtaatggt
    15241 cggattctta accagtacca tatttccaga accatctaca tagacactca ctatataagc
    15301 agagtggacg gtctttgtat tttctccctc tgtaatgagc tggtctacac tgtaggttac
    15361 attaaactca ttgtcgccag ttggctctac cgtccatatc tgaaatcctc ttacagaaga
    15421 cgatacagga atatctttgc gtactgtatc aacattgaga gcttgaagtt catctgtcag
    15481 atagcctttt agactttcca ttcgattatc aatggactta tcggattgct cccatgaata
    15541 gtagactttc gcaaagttct ctacaaaatt ttctacatga tgagtatcaa cgtattcctt
    15601 ttctatgata gttgtttcgt gaatagtatg agtatctata gctgtaaagt gcttgaatat
    15661 cgcaaagctg aaactaagcc ctaaaagtac ccacaaggca atcacaacct ttttatgagg
    15721 attgacctta tagacacgag gtttcttttc ctttggtatc tgtttttctt tattctgatt
    15781 ttttctaaat ttcatcatta aatcttcctt tctcattgtt tgattcgtcc tgctcccact
    15841 aaatgctgtt gccagtaggg gcttgttaag tcggcataac cgattgggtc gcctgcatga
    15901 aacatacggt tattgccaag gtatatccca acatgagtaa tataagagcc agcgttatag
    15961 gtagaatgaa agaaaaccaa atcgccagct tgtgcttccg atagtgggat atgctgggtc
    16021 acatcatatt gctgttgtgc ggttcgtggt aagttaattc cagcttttcc atacgtccat
    16081 tgtgtcagtc cgctacaatc aaaagaagta gtcggggaag ctccaccgta aacgtatcgc
    16141 cagccctcat atttcagtgc ttcgtccatg atggcttgta ccgtatcatc atcaaactct
    16201 gttgtgacaa gatactgcgt taccagttgc acataaaaca tattgccata gttgtatcgc
    16261 cagcccccat tgataggtat ggctatggga ttggggtaag acactttttc gccacctgaa
    16321 tactcttttg agaaactttg agccagttca aaggtatatt tatttccacg attagccaca
    16381 taccctaaga aaccaccacc ataattgtag gactggataa ccgattctaa atctacactg
    16441 agcctttcgc tactggctaa taattcactg aaatacttca cacggttctg ttgcaaagtt
    16501 ttaaatctac tatcaaataa ggtagaataa tagaaaaaga tagcaggagg aatgacgatg
    16561 aatcatttta aaggaaagca atttcagcag gatgtgatta ttgtagccgt gggctactat
    16621 cttcgttata accttagcta tcgtgaagtt caagaaatct tatatgatcg tggcattaac
    16681 gtttctcata cgacgattta tcgttgggtg caagaatatg gcaaactact ctatcaaatt
    16741 tggaaaaaga aaaataaaaa atccttttat tcatggaaaa tggatgaaac gtacatcaaa
    16801 attaaaggaa aatggcatta tttgtatcga gccatcgatg cagatggttt aaccttggat
    16861 atttggttac gtaaaaaacg ggacacacaa gcagcctatg cttttcttaa gcggttagtg
    16921 aagcagtttg atgaaccgaa ggttgtagtc acagataaag ccccctctat tacaagtgcc
    16981 tttaagaaac taaaagaata cggcttttat caagggacag aacatcgtac cattaaatac
    17041 ctgaataatt tgattgaaca agaccatcgt ccagtaaaga gacgcaataa attctatcga
    17101 agtttacgca ctgcctctac cacgattaaa ggcatggaag ccattcgagg attatataag
    17161 aaaacccgaa aagaaggcac tctcttcggg ttttcggtct gtactgaaat caaggtatta
    17221 ttgggaatcc cagcttaaat catagatacc gtaagggatt ttattcttta tttaaaactt
    17281 tgcaacagaa ccaactagac tgtgcaaaat agagacactt gaaaaatcta tcgggggtca
    17341 gaaacttaat agtttttgac acataaacaa aatcgcgata taatggggtt gagagtgttt
    17401 cataaactta tgtcataaag ttaaggagat ttcgcatgat tttagagaat aaattgggat
    17461 taaccaatca agttgagttg gctaaggttg aagaaaaact cagtaaacaa aaagcgaaag
    17521 aattgtacga ttgtggaaaa ataaatgaaa tcgaagttgg tacatttaaa ggtttatctg
    17581 aaattcatga atttctattt tcggatattt acgattttgc cggaaaaatc cgatcggtca
    17641 atattgcaaa aggaaatttt cgatttgcac cagttatgta tttagaacat tcgttacagc
    17701 atattgatca gatgccacaa acttcttttg aagagattat caagaaatat gtggagatga
    17761 atatcgctca cccatttcga gaaggaaatg gccgaagtac tagaatttgg ctggatctca
    17821 ttttaaagga agaattgcaa aaagttgtag attggaactt aatcgataaa gcagactatc
    17881 tttctgcaat ggagcgaagt cccataaacg atttggaaat acgctatcta atttccaatg
    17941 ctctgactga taaaattgac gatcgcgagt tatatatgaa aggaatcgat gtcagctatt
    18001 tctatgaagg gtattctgag tatacgatag atgatttgta aaagtaggtg gaccaaatga
    18061 cgaaatatgg atatgcacgt gtctcaaccg cttctcaaag tacaaaagaa caaattcaac
    18121 agttgcttga gaacggcgta gagaagaaga atattttttc agaaaaattt acgggtacaa
    18181 aacgggatcg gccaaaattc aatcagctgt tggctggtct gaagcctgga gatgaaatag
    18241 ttgttacgaa gcttgatcga tttgctcgta acacgagaga agcattagag atcattgaac
    18301 ctttactcga tgataacgta acgatcaagg ttttgaatct aggaacgatt gaaaatacgt
    18361 ccatggggcg aatggtgaca cggacgttgt tgagtgtagc agaaatggaa cgtgatatga
    18421 ttgtggaacg tacccaagaa ggaaaaatgt ttgcaagaaa gaataatcct aatttcagag
    18481 aaggacgtcc gaaagcaaca atcacaccga agaagaggca cgcatatgaa ctg
//
