LOCUS       NZ_CP116484            18808 bp    DNA     circular CON 27-FEB-2024
DEFINITION  Escherichia coli strain GTEN_24 plasmid unnamed4, complete sequence.
ACCESSION   NZ_CP116484
VERSION     NZ_CP116484.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11230615
            Assembly: GCF_028215995.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18808)
  AUTHORS   Yorki,S., Shea,T., Worby,C.J., Cuomo,C., LaRocque,R.C., Manson,A.L.
            and Earl,A.M.
  TITLE     Comparison of long and short read metagenomic assembly for
            low-abundance species and resistance genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18808)
  AUTHORS   Yorki,S., LaRocque,R.C., Worby,C.J., Shea,T., Earl,A.M.,
            Walker,B.J., Manson,A.L., Sridhar,S., Turbett,S., Becker,M.,
            Oliver,E., Bronson,R., Leung,D., Knouse,M., Hagmann,S., Kelly,P.,
            Harris,J. and Ryan,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2023) Infectious Diseases & Microbiome, Broad
            Institute of MIT and Harvard, 75 Ames St., Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/27/2024 02:21:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,977
            CDSs (total)                      :: 4,853
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 69 of 177
            Pseudo Genes (incomplete)         :: 118 of 177
            Pseudo Genes (internal stop)      :: 28 of 177
            Pseudo Genes (multiple problems)  :: 33 of 177
            Pseudo Genes (short protein)      :: 2 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116484.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..18808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="GTEN_24"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: Massachusetts"
                     /collection_date="2018"
                     /collected_by="Massachusetts General Hospital"
     gene            complement(join(18592..18808,1..488))
                     /locus_tag="E5Q65_RS24640"
     CDS             complement(join(18592..18808,1..488))
                     /locus_tag="E5Q65_RS24640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067858.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(<552..884)
                     /locus_tag="E5Q65_RS24645"
                     /pseudo=""
     CDS             complement(<552..884)
                     /locus_tag="E5Q65_RS24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /name="IS6-like element IS26 family transposase"
     gene            1119..1979
                     /locus_tag="E5Q65_RS24650"
     CDS             1119..1979
                     /locus_tag="E5Q65_RS24650"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            <2129..4390
                     /locus_tag="E5Q65_RS24655"
                     /pseudo=""
     CDS             <2129..4390
                     /locus_tag="E5Q65_RS24655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241615.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            4445..4708
                     /locus_tag="E5Q65_RS24660"
     CDS             4445..4708
                     /locus_tag="E5Q65_RS24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001714223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072657.1"
                     /translation="MSNTSYKQIIPATDWYFRHDNVSGVAGKSTVYQLAAWALKENGEV
                     VGLVTVRDDNGRPKLVTPPPVPGDYLHKEQLTDDEKEWAKRR"
                     /name="hypothetical protein"
     gene            complement(4878..6203)
                     /locus_tag="E5Q65_RS24665"
     CDS             complement(4878..6203)
                     /locus_tag="E5Q65_RS24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="WP_000201787.1"
                     /translation="MATKKYELTKEYFFHGEFWHQLDDNKGRFSARIEYSPYHGLILDY
                     CISDSESPRTCEILYGVLNTGERCTLIGKFDFTQGNIHFDKGIIHTGRHGFPIMLFNDF
                     YAPDSKIEYCDLSLHGLQEFIHPHGFFTQLKHLEHPIFIAKGNHWTLQLVNHVSFSVIG
                     DDLLNIINCQNKAALENIIHQLKKTKELYPDAFFSIRKELVFYFRIKSSNDLGIEDHIS
                     KCWDISGLFSILLNKPTLPEEINIKFKGNGSKTPCLLTTGFEQRTIDLALREIKHQLLP
                     INRKHINLGKIFCKWFKIAERYMPLTITYQYETGFRTLHQAHTDIILFATQLEAINKTI
                     GGSKNEKYMKPINEYASLFLIQEIEMFFKKFNNKSIGENIATLRNELAHVDRKKELMNI
                     LTIGDYVKIGNYLKTIVTSYLLSDLGINNIIIEKYQAQTIQE"
                     /name="HEPN domain-containing protein"
     gene            complement(6254..7045)
                     /locus_tag="E5Q65_RS24670"
     CDS             complement(6254..7045)
                     /locus_tag="E5Q65_RS24670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="WP_000537390.1"
                     /translation="MGTTKDWVIQVEESRREEWIRERLSSPDLEEDSEEWQLLEKDYDE
                     YQDFLSDMAMEEYETEKWLKQHPHTEIYKIAINLLEQIKEEGKQSTSEVFIKMKIAYIV
                     TIMENCLSEMIKSVVLSHNRYVENAIRNINELKAKNISLSELINKESNANKYVQEYLSD
                     ILYHRIQLVVEIYKAVLQPKQYPRFPLKNINELMKLRHDIVHRNGKTKTTDEKIHTFNT
                     ATLNDAFKVVEEFLNNMMNLISDAVEHHENEQIARDLEDEF"
                     /name="HEPN domain-containing protein"
     gene            complement(7252..7827)
                     /locus_tag="E5Q65_RS24675"
     CDS             complement(7252..7827)
                     /locus_tag="E5Q65_RS24675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="WP_001505426.1"
                     /translation="MTQSEMLKLIARHGYNVGFGAKKNFATYDLICKASGWISFISLAV
                     GVFALFIPELATNVISAILIIFGVATMYIQFYDSEKQSYESAGIEQTKIFNELEVIYRN
                     VKSDANFDYQHTQQSIDAIMSRFYGTVISKQIFGSNWYAHYKFYCEFEKTWVEEELNLT
                     LKDKIPLSLCFLAGLAIIFIIAVIWKML"
                     /name="SLATT domain-containing protein"
     gene            complement(7835..9163)
                     /locus_tag="E5Q65_RS24680"
     CDS             complement(7835..9163)
                     /locus_tag="E5Q65_RS24680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="WP_000091038.1"
                     /translation="MSRRELFSEFLENLNLDLKQAKKISYHYRKITKSLNLAFRGTSSR
                     VANRLKVGSVGRHTAIKGISDLDMLYIMPPNQYEYYNRKDNGQAALLTDVRNILAEEYP
                     DQTVKKDRLVVQIIFKNFYVEVQPVFRQDDGSFKFPESYNGGAWRITKPLHEKAAMTAF
                     SRDKSNNLRKLCKMIRAWKNLHGVNMGGLLIDTLAYRFLSSTSDYDNTGNGSLGALARD
                     FFEYLSNEEKRERYLALGSNQYVRVKSPWFGRAAKHAYELCCDALDAEGSASENDLWRE
                     VFGRAFPRRKVGIMEASLGLESHAADAAPWIDTEEFIEDKYPVDIRHSLNLDCTVTQDG
                     FPPRSLREMLTRGFRLSARKSLLFRADLTEMEAEEPYTVMWKVLNVGDEARRRNMIRGQ
                     IVSDGGYCTKKETTDFRGDHVVECYVIKNEVVVARAQIEVPIN"
                     /name="nucleotidyltransferase"
     gene            9784..9867
                     /gene="dqlB"
                     /locus_tag="E5Q65_RS24685"
     CDS             9784..9867
                     /gene="dqlB"
                     /locus_tag="E5Q65_RS24685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_272056275.1"
                     /translation="MMNLLIIVLRAVLAIANALIAIQKLIE"
                     /name="dqlB"
     gene            10428..10523
                     /gene="dqlB"
                     /locus_tag="E5Q65_RS24690"
     CDS             10428..10523
                     /gene="dqlB"
                     /locus_tag="E5Q65_RS24690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_000609146.1"
                     /translation="MINLLIIVLRAVAALANALIAVLELIRQFID"
                     /name="dqlB"
     gene            10590..10769
                     /locus_tag="E5Q65_RS24695"
     CDS             10590..10769
                     /locus_tag="E5Q65_RS24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000347021.1"
                     /translation="MDFMSILGRCLLLPFPVAMFVGCLFPGIDDRFTGMFMSFIVGCLS
                     WYITKPKPQKAEVA"
                     /name="hypothetical protein"
     gene            complement(10885..11529)
                     /locus_tag="E5Q65_RS24700"
     CDS             complement(10885..11529)
                     /locus_tag="E5Q65_RS24700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001503708.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000203199.1"
                     /translation="MTSNAPTNFVRAYLRASTAEQDASRALETIEAFARERGLIICSYY
                     IENESGSRLDRPELFRLLRDCQQNDILLIEDVDRLSRLSGEDWNTLKKMIRQKDIRVMA
                     VNVPTTWINSGMSEFDSRLFAAINDMLLDMLAAVARRDYEQRRERQKQGIEKARKDGKY
                     KGRKPNQARHDAIIRLIESGSSWTQVQKVLGCSRGTISSAIKRKSLQSSGE"
                     /name="recombinase family protein"
     gene            11824..12444
                     /locus_tag="E5Q65_RS24705"
     CDS             11824..12444
                     /locus_tag="E5Q65_RS24705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864791.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_000864791.1"
                     /translation="MKVISFLNPKGGSGKTTAVINIATALSRSGYNIAVVDTDPQMSLT
                     NWSKAGKAAFDVFTAASEKDVYGIRKDLADYDFAIVDGAGSLSVITSAAVMVSDLVIIP
                     VTPSPLDFSAAGSVVTVLEAQAYSRKVEARFLITRKIEMATMLNVLKESIKDTGVKSFR
                     TAITQRQVYVKSILDGDSVFESSDGAAKGEIEILTKEIVSTFE"
                     /name="ParA family protein"
     gene            12496..12726
                     /locus_tag="E5Q65_RS24710"
     CDS             12496..12726
                     /locus_tag="E5Q65_RS24710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partition protein ParG"
                     /protein_id="WP_000051063.1"
                     /translation="MSLEKAHTAVKKMTFGENRDLERVVTAPVSSGKIKRVNVNFDEEK
                     HTRFKAACAKKGTSITDVVNQLVDEWLKENE"
                     /name="plasmid partition protein ParG"
     gene            12742..13053
                     /locus_tag="E5Q65_RS24715"
     CDS             12742..13053
                     /locus_tag="E5Q65_RS24715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001423339.1"
                     /translation="MDKNVVDALKTLLEALREEVVTEVTSKLNPSASHIPEENSKQMTA
                     KARLLNFRLTEAYEEILEAEAIRTGQSKTTVLKAALAAFDSLDENRKNHWLLESAKLG"
                     /name="hypothetical protein"
     gene            13488..14084
                     /locus_tag="E5Q65_RS24720"
     CDS             13488..14084
                     /locus_tag="E5Q65_RS24720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="WP_040121203.1"
                     /translation="MASDNRYSINFTLSVLEKTEASKLIKEHEKQEHIEPYGYILLIKK
                     AALLVLSFAALFDFIILFGLIALAPKDPGYVERAKFLSIITFTLIAIFIGLLYIYDYLM
                     KKVMRTNYLSEIRDSELMLRLKINRSYIEQIMKIGSSKVFWNNIKKTYREKGFLFIQTK
                     ENRCIIIPERVFKNGEETEKLYNFVKEQIAKNTIE"
                     /name="YcxB family protein"
     gene            complement(14136..14492)
                     /gene="ddp1"
                     /locus_tag="E5Q65_RS24725"
     CDS             complement(14136..14492)
                     /gene="ddp1"
                     /locus_tag="E5Q65_RS24725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 3"
                     /protein_id="WP_025840293.1"
                     /translation="MKYLVKEFINEKYTKVVNILKDNLKEHYHVFYCVRLSEILFPASE
                     YGTEQFFSDFEKINSISLPVLVFDLKEGVPVIVISLDDVSYCDVLDEMDIDFMKCDSLA
                     ELLTSDKLDALFKD"
                     /name="ddp1"
     gene            complement(14632..15075)
                     /locus_tag="E5Q65_RS24730"
     CDS             complement(14632..15075)
                     /locus_tag="E5Q65_RS24730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001074380.1"
                     /translation="MAKIYQFPQGDERGKFRKEIARERKKRFAVKTGSIFVKWLGWGWF
                     YLRLLAASVLHFVSVFVLAILGAFKWAVFWLGGILCVITWYHLDHQLWTPQNLTIPVIT
                     ATWILGLLAEPLMELLNKTMPWHKLLVPEPADESTENSGEISQ"
                     /name="hypothetical protein"
     gene            complement(15079..15921)
                     /locus_tag="E5Q65_RS24735"
     CDS             complement(15079..15921)
                     /locus_tag="E5Q65_RS24735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435061.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_000435061.1"
                     /translation="MEQIKLHKQGGAAVIIIPASMIENTGWEDGTLLDVLEINEALHIR
                     RKTESGALATKACPSLFSSTMRHVNYDKIIRYNSGLNLLVNELSCSARRVFFICLYYYF
                     ENKLESDKSLLIRADKYAEFVGVDRSVAYKQMKDAADFFSRNKKLITHCDYISNEGLLR
                     VMFSSKTINYITAIDSRKYQVTELPLKSALSLQGRYSWPLYQLIKFSHLKSFTIGVDEL
                     KKEIGYIQNQKFSFFKRDGINIAIDEITERTDVKNIKCVNAERQGRRVSKVRFEIEMR"
                     /name="replication initiation protein"
     gene            complement(15942..16781)
                     /locus_tag="E5Q65_RS24740"
     CDS             complement(15942..16781)
                     /locus_tag="E5Q65_RS24740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722379.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_021526660.1"
                     /translation="MNLLSVTKKTKIRHRNEINSTFSSLPLSARRILFMAMAQIDSREE
                     LSEGATFRITAREYAFIADIDVTGAYRQLKEGAEELQASVIRIPRNQLLTPQGNQSSGH
                     TKKRGKLPSDAVRLMNLTAFCDYVESEGYIDIAFSHVIEPYITMLKDSYTTQVLISSVR
                     LADQNSNMLYQFIRKQYSSGKKTFFDIEVDVLKDELELFRIDNGEKVYLYQNFKDFNKV
                     FLQKNIEKINQYTEINHLEVKIVERVARRASKLRFSYKIDKESEGLDTRIPYGFRGA"
                     /name="replication initiation protein"
     gene            complement(17791..17922)
                     /locus_tag="E5Q65_RS24745"
     CDS             complement(17791..17922)
                     /locus_tag="E5Q65_RS24745"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001378418.1"
                     /translation="MALSDIALKRSVTRSERFSRFVVVGYGLTVFNAAQRFLAALLL"
                     /name="hypothetical protein"
     gene            18014..18265
                     /locus_tag="E5Q65_RS24750"
     CDS             18014..18265
                     /locus_tag="E5Q65_RS24750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WP_000121743.1"
                     /translation="MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCV
                     PPKLYAYYRELAEDAELNAVADERMKNPEIVKVNLDDL"
                     /name="plasmid stabilization protein"
     gene            18255..18536
                     /locus_tag="E5Q65_RS24755"
     CDS             18255..18536
                     /locus_tag="E5Q65_RS24755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694627.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000220560.1"
                     /translation="MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLH
                     GHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGRRESSEVYQIADLR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     ARG             1120..1976
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(14633..15075)
                     /name="IncX1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.973"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cggtgttcaa cgtcagacgg gcaccggcct tcgcgtttga gcagagcaag cgcgcgacca
       61 taggcgggcg ctttatccgt gttgatgaat cgtgggatct gccacttctt cacgttgttg
      121 aggattttac ccagaaaccg gtatgcagct ttgctgttac gacgggagga gagataaaaa
      181 tcgacagtgc ggccccggct gtcgacggcc cggtacagat acgcccagcg gccattgacc
      241 ttcacgtagg tttcatccat gtgccacggg caaagatcgg aagggttacg ccagtaccag
      301 cgcagccgtt tttccatttc aggcgcataa cgctgaaccc agcggtaaat cgtggagtga
      361 tcgacattca ctccgcgttc agccagcatc tcctgcagct cacggtaact gatgccgtat
      421 ttgcagtacc agcgtacggc ccacagaatg atgtcacgct gaaaatgccg gcctttgaat
      481 gggttcatgt gcagctccat cagcaaaagg ggatgataag tttatcacca ccgactattt
      541 gcaacagtgc cgttacgacg ggaggagaga taaaaatcga cagtgcggcc ccggctgtcg
      601 acggcccggt acagatacgc ccagcggcca ttgaccttca cgtaggtttc atccatgtgc
      661 cacgggcaaa gatcggaagg gttacgccag taccagcgca gccgtttttc catttcaggc
      721 gcataacgct gaacccagcg gtaaatcgtg gagtgatcga cattcactcc gcgttcagcc
      781 agcatctcct gcagctcacg gtaactgatg ccgtatttgc agtaccagcg tacggcccac
      841 agaatgatgt cacgctgaaa atgccggcct ttgaatgggt tcatgtgcag ctccatcagc
      901 aaaaggggat gataagttta tcaccaccga ctatttgcaa cagtgcctta taggttaatg
      961 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     1021 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1081 cctggtaaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa cattttcgtg
     1141 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     1201 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     1261 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     1321 gcacttttaa agttctgcta tgtggtgcgg tattatcccg tgttgacgcc gggcaagagc
     1381 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     1441 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     1501 gtgataacac tgctgccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     1561 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     1621 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt
     1681 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     1741 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     1801 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     1861 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     1921 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     1981 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     2041 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     2101 tttcgttcca ctgagcgtca gacccctaga tcgtaccatt caccagaacc ggttgctgaa
     2161 actggcgcgg gaaggccgaa agatgagtag ccgggatttg acccgatttt cagctgcccg
     2221 gcgtcatgcg atcctggtgt gcgtgctgga agaagccaga gccacactga cagacgaagt
     2281 gattgagctg catgagcgaa tgctgaatag tcttttcagc aaagccaaac gaacacaggc
     2341 tgagcgcctt cagcagaccg gaaagctgat ccagtcaaaa ctgaggcagt acatcgatgt
     2401 cggtcaggcg ctgtctgatg cccgtgattc cggtggcgat ccctggctcg caatagaaaa
     2461 catactccca tgggctgaat ttgtcgccag tctggatgaa acacgccatc tcgccagaaa
     2521 aaataatttt gacccgctgc atattattac cgagaaatac agcacattac ggaaatatgc
     2581 cccacgcatg ttgtccgctt tacagttagt cgcaaccccg gcagcgcaac ctctggctga
     2641 tgcactggtt gtgatcaagg atatgtaccg gaagcagtca cgtaaagttc cggcggcagc
     2701 gccgcttgag tttgtccctg aaagctggcg caaggtggtg attacacctg tgggtattga
     2761 ccggcagtac tatgagtttt gtgcactcag cgagcttaag ggggcgttgc gctccgggga
     2821 tatctgggtt aaaggctcgc gccgctacaa aaattttgat gattacctca tacccgagaa
     2881 ggactttgac aaactcacac cggcactgcc actgcccgta tctgctgatt atcatgagta
     2941 cattaccagc cgtatgaccc tgcttcagtc cagacttgaa gaggtcaatg ccatggctgc
     3001 ccttggtgaa ctgcccgatg tggaaatatc cgacaggggc gtaaaagtct ctccgctgga
     3061 taactgcgtt ccggcgcagg tttcaccgct ggcagagctg gtttacagca tgctaccgcg
     3121 acctaaaatc acggaaattc tcgatgaggt aaacagctgg acgacgttta cccgacattt
     3181 ttcgcatata aaaaatgaca ttacccgtcc cgatacccgc ttgctcctca ccaccattct
     3241 tgcggatggc atcaatcttg gcctgaccaa aatggcagaa gcctgccccg ggagcaccaa
     3301 atcatcactc gaagatattc aggcatggta catccgtgac gaaacctatt cagccgctct
     3361 tgcagagctg gtaaatgcgc aggggaaaag accgttggcg gcattttggg gagacggaac
     3421 aacgtcatca tcagatggac aaaatttcag aaccggcagc tcaggccgct acgcagggca
     3481 ggttaacccg aaatatgggc aagagcctgg acgtcagttt tatacccata tttcggatca
     3541 atacagcccg ttttacacct gcataatcag tcgggtcagg gattcaaccc atgttctcga
     3601 tggcttgctg taccacgaaa gcgacctgga aatcagggaa cattacaccg ataccgctgg
     3661 tttcaccgat catgtctttg ccctgatgca tctgttggga tttgcgttct gtccgcgaat
     3721 cagggatctc cacgacaaaa aactgtttat caaagggaaa gcagagaaat acccggcgct
     3781 tcagtcactg atatcaacga ccagcctgaa tctgaaagag atcgaaatcc actggcgtga
     3841 agtgctgcgt ctggcaacct cgataaagca gggaaccgtg acggcatccc tgatgctgaa
     3901 aaagcttgcc agctatccca agcaaaacgg acttgccaaa gcactgagag aaattggccg
     3961 cattgagcga accctgttta tgctcgactg gttccgcgac ccggctctgc gtcggcgggt
     4021 acaggcgggg ctgaataaag gcgaagcccg taacgcgctg gcgcgtgcgg tatttatgca
     4081 ccgactggga gaaatcaggg acagaaaacc ggaaaatcag agctatcgcg ccagtggact
     4141 gacgctgctg acggctgcca tatccctgtg gaataccgtc tatatggaaa gagcggtcga
     4201 tgccctgaag cgtaaagggg tgaagatcaa cgagcaactg ctgtcgcatt tgtcgccgtt
     4261 gggctgggaa cacatcaatc tgacaggtga ttatatctgg aaaagcaacc ggataccggc
     4321 ttctggtaag tttcgtcggc taagaccagc taaagtcgaa aggtcaaaaa acagccttaa
     4381 cgtacaataa ttttcgatat ccaaactgac ccctatatta tcattttgtt aaggaagaaa
     4441 atccatgagt aatacatcct acaaacaaat tatccctgcg acagactggt atttccgtca
     4501 cgataatgtc tccggtgtgg caggaaagtc aacagtatac caactggctg catgggcgct
     4561 taaagaaaat ggtgaggtag ttggtctggt gacggttcgt gatgataatg ggcgccctaa
     4621 actggttact cctccccctg tccctggtga ttatttgcat aaagaacaac tcaccgatga
     4681 tgaaaaagag tgggcgaaga gacgctaaac tatattcata taaagcctct gttctagagg
     4741 ctttattagc atgtttagtt gcactggagt tgataatggc ctcaatgcgc aaattgaggt
     4801 aaattgatat tgattttact tttccgttgc tcgtttctcg tcttattata tttaaatgat
     4861 aattgtataa tattttatta ctcttgaatt gtctgagcct gatatttttc aataataatg
     4921 ttattgattc ctaaatctga tagcagataa gatgttacaa ttgtttttag gtagtttcca
     4981 attttaacat aatcaccaat cgtcaatatg ttcattaact cctttttgcg gtcaacatgt
     5041 gctaattcat ttctgagtgt ggcaatattt tcaccaatag atttattgtt gaatttctta
     5101 aaaaacatct ctatctcttg aataagaaac aaacttgcat attcattaat gggtttcatg
     5161 tatttttcat tttttgagcc acctattgtt ttgtttattg cttctaattg agtcgcaaac
     5221 aatataatat cagtatgagc ttgatgcagt gttcgaaaac cggtttcata ctgatatgtt
     5281 attgtcaatg gcatatatct ttctgctatt ttgaaccatt tgcagaatat cttcccaaga
     5341 ttaatatgct tcctgtttat tggaaggagt tgatgtttta tttctctcag cgctaggtca
     5401 atagttcttt gttcaaaccc cgttgttaag agacatggtg tcttcgaacc atttcccttg
     5461 aactttatgt ttatttcttc aggaagagtt ggtttgttca gaagaattga aaataacccg
     5521 gaaatatccc agcactttga tatatggtct tctattccta aatcattaga tgattttatc
     5581 ctaaagtaga aaacaagttc ttttctaatg gagaaaaaag catcagggta taattctttt
     5641 gtctttttta gttgatgaat tatgttctcc agtgcggctt tgttttggca atttattatg
     5701 tttaatagat catcaccaat tacgctaaaa gatacatggt tgacaagttg aagagtccaa
     5761 tggtttcctt ttgcgataaa tattggatgt tctaaatgtt tcaattgagt gaaaaaaccg
     5821 tgagggtgga taaattcttg caatccgtgc aaggataaat cacaatattc tatctttgag
     5881 tcgggagcat aaaaatcatt gaatagcatt atgggaaatc catgccttcc agtatggata
     5941 atccctttat caaaatgaat attcccttgt gtgaaatcaa atttcccaat tagagtacat
     6001 ctttctccag tatttaagac tccatataat atttcacatg ttctggggct ttcactgtca
     6061 gaaatacaat aatctagaat cagaccatgg tacggtgaat attcaattcg tgctgaaaat
     6121 cttcctttgt tatcatcaag ttggtgccaa aattcaccat gaaaaaagta ttcttttgtt
     6181 agttcgtatt tttttgttgc catgtactgc tccatttgtt tctgagaatc cttcagaata
     6241 gtaaaaatac tactcaaaac tcgtcttcaa gatctctggc tatctgctca ttctcatgat
     6301 gctcaacagc atcactgatc aaattcatca tgttattaag aaactcctct acaactttaa
     6361 acgcatcatt gagcgtagct gtattgaatg tatgtatctt ttcatccgtt gttttcgttt
     6421 tcccgttacg atgaactatg tcatgcctca gtttcatcaa ttcattgata tttttgagtg
     6481 gaaaacgtgg atattgctta ggttgcagga ctgctttata tatttcaact accagttgga
     6541 ttctatgata aagaatatct gaaaggtact cctgtacata tttgtttgca ttagactctt
     6601 tatttatcaa ttcagacaga gagatattct ttgctttcag ttcatttatg tttcttatgg
     6661 cattttctac atatctgtta tgtgacaata caacgctttt aatcatttcg ctaagacagt
     6721 tttccattat tgtcacaatg tatgcgattt tcatttttat gaaaacttca cttgtagatt
     6781 gttttccctc ttcttttatt tgttctaaaa ggtttattgc tattttataa atttctgtat
     6841 gtggatgctg ttttaaccat ttttcggttt catattcttc catagccata tcagaaagaa
     6901 aatcctgata ctcgtcatag tccttttcaa gtaactgcca ttcttcagaa tcttcctcaa
     6961 gatctgggct ggaaagccgc tctcgtatcc attcctcacg gcgactttcc tcgacttgta
     7021 taacccaatc ttttgttgtt cccacagtac cttccttatg ttcattgcta aatatcaata
     7081 gtcactttat cggagactac gtgaaaaatc ctatcttttc cccctgcctt tttattagat
     7141 ttatagctca tatccaaact caccccttat agaacgtaca ctggataatg ataccggtta
     7201 tacatcgcat atttgccagt cagcttgctt aatttttcat cggctgcagc atcagagcat
     7261 tttccagatg acagctataa tgaaaatgat tgccagaccg gcgagaaagc agagcgaaag
     7321 cgggattttg tccttcagtg tcaagtttag ttcttcttca acccatgttt tctcaaattc
     7381 acagtaaaat ttataatgtg cgtaccagtt acttccgaag atctgtttgc tgatgaccgt
     7441 gccgtaaaac cgggacatga tagcgtcgat ggattgttgt gtatgctgat agtcaaagtt
     7501 tgcatcgctt tttacgttgc ggtaaataac ttcaagttca ttaaaaattt ttgtctgctc
     7561 aatccccgca ctttcataac tctgcttttc cgagtcataa aactgaatgt acattgtagc
     7621 aacaccaaat ataattaata tcgcggaaat tacgtttgtg gctagctcag gaataaaaag
     7681 agcgaacaca ccgacagcaa gagaaataaa gctaatccaa ccggatgcct tacaaattag
     7741 atcataggtg gcaaagtttt tcttagcgcc aaatccaacg ttataaccat gtctggcaat
     7801 taatttcagc atctctgatt gcgtcataaa aacctcagtt tatgggaact tcaatctggg
     7861 cccgggcaac cacaacctca ttttttatca catagcattc taccacgtga tctccgcgga
     7921 agtcggtggt ctctttcttt gtacagtagc cgccgtccga aacgatttgt ccccggatca
     7981 tatttcttct gcgtgcttcg tcacccacgt tcagtacctt ccacataacg gtgtagggtt
     8041 cctctgcctc catttctgtc aagtctgccc ggaaaaggag agatttccga gcggagaggc
     8101 gaaatcctct agtaagcatc tctctcaggc ttctgggagg gaaaccatct tgggttacgg
     8161 tacagtcaag gttgagtgag tgcctgatat ccaccggata tttatcctct ataaattctt
     8221 cggtatcgat ccagggagct gcatctgccg catgcgattc cagaccaagg ctggcttcca
     8281 taatacccac cttacgtctg gggaaggctc gtccgaacac ttcgcgccag aggtcatttt
     8341 cactggcaga cccctcagcg tctaaggcat cacaacataa ttcatacgca tgtttcgctg
     8401 cccggccaaa ccagggagat ttaacacgta catactgatt actgcctaac gcgagatacc
     8461 tttccctttt ctcctcattt gacagatatt caaaaaagtc tctggcgagg gctcccaggc
     8521 tgccatttcc ggtattgtca taatctgatg ttgatgaaag aaatcgatac gccagtgtat
     8581 ctatcagtaa gccccccata ttgacgccat gcaggttctt ccaggctctg atcattttac
     8641 acagcttgcg cagattattg gatttatctc gcgaaaatgc ggtcatagcc gccttttcgt
     8701 gaagaggttt tgtaatgcgc catgctcccc cattatagct ttcagggaat ttaaagctgc
     8761 catcatcctg tctgaataca ggttgaacct cgacataaaa gtttttaaag atgatctgaa
     8821 caaccagtct gtcctttttc accgtctggt caggatattc ttccgctaaa atgtttctga
     8881 catctgtaag caatgcagcc tgaccattat ccttacggtt ataatattca tactgattgg
     8941 ggggcataat ataaagcatg tccagatcgg atatgccttt tattgcagta tgtctgccta
     9001 ctgaccccac tttcaagcgg tttgctaccc gggaactcgt tcctctgaac gcaagattta
     9061 atgacttggt tatttttcga tagtgatagc tgatcttttt tgcctgttta aggtcaagat
     9121 tcaaattttc aagaaattcg ctgaacaatt ccctgcggct catggtattt cctttggtat
     9181 gtgaaacatt agcttacatt atcattacac cttataaaaa ccaagatctt gggaggtgag
     9241 cgttctgcat ggctttatga cttaaacttt tacaggacgg tctataaaaa taattcatga
     9301 agtcatttct gctctgccta ctacttcttc aatgaagggg gaaaaaacgg aagtacagct
     9361 gaacgacaag cacaaaaaaa cccgactggt aatttggctt ttttgtgttt tccagccttg
     9421 agttggtggc tctgactgga ggaagatact ctacacccaa cactcatagt attatggcac
     9481 tagacacctg ttgtctagtt tgacttcaga ttgtctgtca atggtaattt ctttaaatga
     9541 attttatgca tgcatctgta taatctttgt gttggcgctg tacagtgtgt ctcccttgct
     9601 tctaacccct gcgctatctg gggatgaacg aattaaagtc ctggctgaac aaacgggtgg
     9661 tgctgtgttc agtgctctgg tagagggaag gtactctaca tccaacaatg tgagagagcc
     9721 gttgtgacag ggatgtatga gcaagctacg gtgacttcct ccagtatcaa cctagagaaa
     9781 cggatgatga atctgttaat catcgttctc agggctgtgc tcgccattgc aaacgcgctg
     9841 attgccattc agaaactaat cgagtgattg atggcttaac taagggagag acgtttaccg
     9901 cttcctgccc tttaacaaag gagtgtttat gagttttgaa caattattta cactaacgac
     9961 ccaacgtcgt tgggtttgcc gcagaaaatt tattcattta aaatagatct aaatgaagcg
    10021 ggaaactgca gacgtaaaaa aaccgactgg tgaggtcggc ttttttacag ttccggtacg
    10081 agctggtaac tcgcccggaa gagaaactct ctacaactga caacagtatg ataatccgtg
    10141 aagcctgatt atgcaatatg acaggcggtg actacgcgtt aacggtttgc ttgaaagtta
    10201 gataaacgta taatcagagg gttagtgctg tggtgagtct ttccctgcat tccgctaaat
    10261 gcggcatgaa cagcttcaaa gtcctgacag gtaatacagg tggtgctggt tatctgtgct
    10321 ctggtaggag tgagactctc tacaactgac aagacgaaga gaagccgtgg tgacaggcag
    10381 cgtaagagca agccacggtg tctttactcc atttatggag aaaacggatg attaacctgt
    10441 taatcatcgt tctgagggcg gtagctgcgc ttgcaaacgc gctgattgca gttctggaac
    10501 tgatccgtca gttcatcgat tgatgactgc ggaaacgtaa ctaagggcag gaagtgcacc
    10561 gcttcctgcc ctttaacaaa ggaggtgtta tggattttat gagcattttg gggcgctgtt
    10621 tacttcttcc tttccctgtt gcgatgtttg tcggttgcct gtttccaggc atcgatgatc
    10681 gttttactgg aatgttcatg agttttattg tcggttgtct atcgtggtat atcaccaagc
    10741 caaaaccgca aaaggctgag gtcgcgtaag cggcctttta ttttgatact cattagttac
    10801 aacattcact ttgcttttta aaaatatcca cttcagcacc tcactcataa ataacgtcaa
    10861 ccagtttatt gagctcaccc taccctactc accggaggat tgcagggatt tacgttttat
    10921 tgcgctactg attgtgccgc ggctgcaccc tagcaccttc tgtacctgtg tccaggagct
    10981 gccactttct atcaacctga taattgcatc atgtctggct tgattaggtt tacgcccttt
    11041 atatttgccg tctttccttg ctttctctat cccctgtttc tggcgttccc gccgttgttc
    11101 atagtccctt ctggcaacag cggccagcat atccagcagc atatcgttaa tggctgcaaa
    11161 caaccggctg tcaaactcac tcataccaga attaatccag gtcgtcggca cattcacggc
    11221 cataacccgg atatctttct gacggatcat tttcttcagc gtattccagt cttcccctga
    11281 cagtcgggaa agtctgtcca catcctcgat aagcaaaatg tcattttgct ggcaatctct
    11341 cagaagacgg aagagttccg ggcggtcaag acgggagccg gattcattct caatataata
    11401 gctgcaaatg atcaggcctc tttccctggc aaaagcctcg atagtttcca gggcacgcga
    11461 agcatcctgt tctgctgttg aagctctcag ataagcgcgg acaaagtttg ttggtgcatt
    11521 tgaagtcata ttacagaacc agttcgcata aggtcacacc actataccca aaatgaacca
    11581 gaaagtggat cgacaggaaa ggcacactct aaaagaacca aaacacaaat aatgagtaga
    11641 tgtgaccttg tcaccatata ctcataaact caaatactca catacacaat acaactcaaa
    11701 tgagtaaaca ctctttaact catatacaca tagactcata ttaaccttta ctcatacaaa
    11761 gagtatggta aactcaaaaa ctcacaggag taattgagta gatacacaat tgagagtttg
    11821 agtatgaaag taatctcatt tctgaatcct aaagggggtt caggcaaaac aactgccgta
    11881 atcaacatag ccactgcgtt gagcaggagc ggatacaaca ttgctgtggt agacacagat
    11941 ccacaaatga gcctgacgaa ctggagcaaa gcaggcaagg cagcatttga cgtatttaca
    12001 gcagcatctg aaaaagacgt ctatggaatc cgaaaagatc tggcggacta tgattttgct
    12061 attgtggacg gagcaggttc gctctcagta atcacctccg cagccgtcat ggtaagcgat
    12121 ctggtaataa tccctgtcac ccccagcccc ctggatttct ccgcagcagg aagcgtcgtt
    12181 actgttctgg aagcacaggc ttacagccgc aaagttgaag cccgctttct gatcacccgt
    12241 aagatagaaa tggcaaccat gctcaatgtg ctgaaagaaa gtatcaaaga taccggtgtt
    12301 aaatccttcc gtacggccat tacacaacgt caggtttacg tgaaatcaat tctggatggt
    12361 gacagcgtgt ttgaatccag tgatggtgca gcaaaaggtg aaatagaaat ccttacaaaa
    12421 gagatagtta gcacatttga gtaattacac atttactcat ataatcaaat aagtataaca
    12481 accggagaaa gcttaatgtc acttgaaaaa gcgcatacgg cagtaaaaaa aatgaccttt
    12541 ggtgaaaaca gagatctgga acgagtagta acagcaccag tatcatcagg aaaaatcaaa
    12601 cgtgttaacg tcaattttga cgaagaaaaa cacacccggt ttaaggctgc atgtgccaaa
    12661 aaaggtacat cgatcactga tgtagtgaac cagcttgtag atgaatggct caaagagaac
    12721 gaataatatc tgaggaatat catggataaa aacgtcgttg atgcgctgaa aacgctatta
    12781 gaagcgttac gggaagaagt ggtaacagaa gtcacatcaa aactaaatcc ttcggcaagc
    12841 catattcctg aagaaaacag taaacaaatg acagcaaaag caagactact gaatttccgg
    12901 ctaaccgaag cctatgaaga aatcctggaa gctgaagcta tcaggactgg tcagagcaaa
    12961 acaaccgttc tcaaagcggc gctggccgct ttcgatagcc tggacgagaa ccggaaaaac
    13021 cactggctgc ttgaatctgc aaagttaggt tagcattatg gctaagaatg atagatatgt
    13081 tgttatggtt gggaataaaa ccatatacag cggtaatcag cgatttttag cctggttagt
    13141 atggttagct cacaggtaca ataaagccat tgcctgtgac aacgggatat ggattgtcga
    13201 gccatcttac tggttgagga ctggaaagga aaaatgataa tgagaatatt cattgtatta
    13261 gctggtcttc tcttgggatg ctggaactta tttgataact acagatccta taaaaaagga
    13321 gtttataaag agcatcgcaa gatggctcct cctgtttatt attatcgggg agatcatact
    13381 tttgtcatac ggatagtaat tgattcactt ttaagtctgg tgattattgg ctttgttgtc
    13441 tggttctggt ttaaaactgc ttaacatcat aacatcgagg aagcaaaatg gcatcagata
    13501 atagatacag tattaacttc acgctatctg ttttagaaaa aacagaagca agcaagctga
    13561 taaaagaaca tgaaaaacaa gagcatatag aaccatatgg ctatatattg cttataaaaa
    13621 aagcagcgtt gttagttctt tcatttgctg cattatttga ttttataata ctttttggtt
    13681 tgattgctct cgccccaaaa gaccctggtt atgtagaaag agcaaaattt ctttccatta
    13741 ttacatttac actaatagct atatttatcg ggttattata tatatatgat tatttgatga
    13801 aaaaagtaat gaggactaac tatttatcag aaataagaga ttctgaacta atgttacgtc
    13861 tcaaaataaa tcgttcatac attgagcaaa tcatgaaaat tggtagttcg aaggttttct
    13921 ggaataacat aaaaaaaaca taccgtgaga aaggatttct atttatccag acaaaagaaa
    13981 acagatgcat aatcattcca gaaagagttt ttaaaaatgg agaagaaacc gaaaaattat
    14041 ataattttgt aaaggagcaa atagcaaaaa acacaataga atgaaaaagg aaaaacaaaa
    14101 aggaagttta agtcgcagag aaggggacat aagactcaat ccttaaataa cgcatccagc
    14161 ttatcgctgg taagcaattc tgccagcgaa tcacatttca taaaatcaat atccatttcg
    14221 tccagaacat cacagtagct gacatcatca agactaatga caatcacagg aacaccctct
    14281 ttgagatcaa acactaagac aggtaatgaa atggagttta tcttttcaaa atcactaaaa
    14341 aactgttcag ttccatattc actggcagga aaaagaatct cacttaatct cacacaataa
    14401 aaaacatgat agtgttcttt aaggttatcc tttaaaatat taacaacctt agtatatttt
    14461 tcgttaataa actctttgac caaatatttc ataaaactct ccaacaagaa ccgactgtag
    14521 gtcatcgggc aaacgttgcg gaatggcgtc agagacgtca ttttacggcg tttgccctat
    14581 cctgcatcgc agtgaaaaag caggaactgg cataacggct gagtagcatc attactggct
    14641 tatctcgcca ctgttctcgg tgctttcatc cgcaggttcg ggaacaagta acttgtgcca
    14701 gggcatagtt ttgttgagca gctccatcag aggctcagcc agcagtccca aaatccaggt
    14761 tgccgtaatc accggtatgg tcagattttg tggcgtccat aactggtgat cgagatgata
    14821 ccaggtgatg acacacaaaa tcccccccag ccagaacaca gcccatttaa acgcaccaag
    14881 aatggctaac acaaaaacag agacaaaatg taaaacactg gcggccagaa gacgaagata
    14941 aaaccatccc cagccaagcc acttaacaaa aatgctccct gttttcaccg caaaacgttt
    15001 cttacgttct ctggcgattt ctttcctgaa tttaccgcgt tcgtccccct gggggaattg
    15061 atagatttta gccatgagtt acctcatttc gatctcaaaa cggactttgc tgactcttct
    15121 accctgcctt tcagcattaa cacatttaat gtttttaaca tctgtccttt ctgttatttc
    15181 atcaatagct atattgattc catctctttt aaagaaagaa aacttttgat tttgaatata
    15241 acctatttct ttcttaagtt catctacacc tatagtgaaa gatttaagat ggctaaattt
    15301 tatcagttga tataaaggcc atgagtatct tccttgaaga gacaaagcag attttagagg
    15361 aagctcggta acctgatatt tacgggagtc tatcgctgta atataattta tcgtcttaga
    15421 cgaaaacata acacgaagca atccctcatt cgaaatatag tcacaatgtg taattaattt
    15481 tttatttctt gaaaagaaat ctgctgcatc cttcatttgc ttatatgcga cagaacgatc
    15541 aacaccaaca aattctgcat acttatcagc cctgatcaat agcgacttat ccgattcaag
    15601 tttattttca aagtaataat ataaacaaat aaaaaagacc cttcttgccg aacatgacag
    15661 ttcattaact aacagattta atccgctatt atatctaatg attttatcgt aattaacatg
    15721 cctcatagta gatgaaaaaa gagaaggaca agctttagta gctaaagctc ctgattctgt
    15781 ctttcttctt atatgaagag cctcatttat ttctagtaca tccaataagg ttccatcttc
    15841 ccatcctgta ttttctatca tactggcagg aatgataatt actgccgcac caccttgttt
    15901 atgtaatttt atttgctcca tagtgtattt acctttatat attaagcacc tctgaaacca
    15961 tacggtattc tggtatcaag tccttcgctt tctttatcta ttttatacga aaaccttagc
    16021 tttgatgctc ttcttgcgac tctctcaacg attttaactt cgagatgatt aatctctgta
    16081 tattgattta ttttttcaat atttttttgc agaaaaacct tattaaaatc cttaaaattc
    16141 tgataaaggt aaactttttc gccattatct attctgaata attcaagttc atcctttaat
    16201 acatctactt caatgtcgaa aaatgtcttt ttaccactgc tgtattgttt cctgataaac
    16261 tgataaagca tatttgaatt ttgatcagcg agtcttacag acgatatcaa aacctgagta
    16321 gtgtaagaat cctttaacat cgtgatatat ggctcaatga catgtgaaaa agcaatatca
    16381 atatatcctt cactttcaac ataatcacaa aacgcagtca ggttcataag cctgactgca
    16441 tcagaaggta atttacctct cttcttcgta tgtccagagc tttgattgcc ctgaggtgtg
    16501 agaagttgat ttcgaggaat gcgaataaca ctggcctgaa gttcttctgc cccctctttc
    16561 aactgccggt aagcacctgt tacatcaata tcagcgataa atgcatattc ccgagccgta
    16621 atacgaaagg ttgccccctc agataactct tcacgtgaat caatctgcgc catagccata
    16681 aacaaaattc ttctggcaga caaaggcaac gatgagaatg tgctgttaat ttcatttctg
    16741 tggcgaattt ttgttttctt tgtaacgctc agtaagttca tacattacct gtggttttta
    16801 agttctgatg gtgaaatcat aaccacaaag gcggaaatat gtaaagtggt ttatccacct
    16861 tatgtatttc caccaaaaca cggatcacat gaaagaattt ttatcattta tattcaatat
    16921 gatagaaatg cgcaggtatc caccttttaa cgtcatacgt taaattagag cactataaag
    16981 aactaaagaa atgaagatac aaagatacga atatctgaat aaaaacagaa aaatgcgtac
    17041 ccatccacct ttcagtgcgt acccatccac ctttcagtgc gtacccatcc acctttcatg
    17101 aaaattacag tgcgtaccta tccacctttc agtgcgtacc catccacctt tcattttgag
    17161 gcgacaaaaa acaacaccta tcttaatgaa attattgata aaaaagctgg cacaatgttt
    17221 gcagaataag ttaaagtaag ttaaagatct taaagtagat ctaccgatct acatttaaga
    17281 tcttaaaaac aagaagttta ataagcaaaa aaacacaaaa aatctgtgga taactctgct
    17341 caaaacaaaa aattcacagc gcctgtggat aactttgcgt aaacccggag gacagatcac
    17401 tctgaaccaa gacaaccaca cataaaaaca atatgctcat tttttaacca ctgtgcgtaa
    17461 gtgcctaatg taatccctgt gctattccgc ttcctcgctc actaactcgc tacgctcggt
    17521 cgttcggctg cggcgagcgg tgtctccttt ctcaaaacca gaagacttca gatcatacga
    17581 tccgttgatt gatccttttt ggatctttca ctgagaagcc ctgaatcgct ttctgccgct
    17641 tcgttcgata caaccatggt gaaaccatgc gaaatcgaaa aaatcgctca gaatgcgata
    17701 cagcgcgttt tagagggtat ttacaaaaca gtcgttgcct tgaggaaatt ttgatcaaga
    17761 ttgaactcct atccggatca tggcaacaga tcacagcaac aaggccgcta aaaagcgttg
    17821 agcggcgtta aacaccgtca aaccgtagcc gactacaacg aaacgggaaa aacgctcaga
    17881 acgcgttaca gagcgtttta aggcgatgtc ggaaagagcc atatctcacc tattctgatg
    17941 tttttctcaa aatgagcact tttaagagtg cttttaaaga tgctattatg ccaatgagac
    18001 aagcggaggg cttatgagct atcaaattct gacaaccaca gcggccagta ttactgacct
    18061 gaaaaaaaat cctatgggaa ccgtagctga aggtgaaggg gacgctgttg cgatcctgaa
    18121 ccgaaacgaa cccgcgttct attgcgttcc accaaaactt tacgcctact atcgggaact
    18181 cgctgaagat gctgagttaa acgctgttgc tgatgagcgc atgaaaaacc cggaaattgt
    18241 gaaggttaac ctggatgacc tatgaactgg cttttgaccg cagagcactg aaggaatggc
    18301 agaaactcgg ccacaccatc cgtgaacaat tcaaaaagaa actggcagaa cggctggaaa
    18361 atccacgcgt acccgcagcc cggttacatg gtcatgctga tcgctataaa atcaaacttc
    18421 gtgcatctgg ctacagactt gtatatcaag tcattgatga gaaagtcgtt ttactcgtta
    18481 ttgccgttgg aagaagggaa agcagcgaag tctatcagat cgcagatttg cgctaagatg
    18541 gcactgttgc aaagttagcg atgaggcagc cttttgtctt attcaaaggc cttacatttc
    18601 aaaaactctg cttaccaggc gcatttcgcc caggggatca ccataataaa atgctgaggc
    18661 ctggcctttg cgtagtgcac gcatcacctc aatacctttg atggtggcgt aagccgtctt
    18721 catggattta aatcccagcg tggcgttgat tatccgtttc agtttgccat gatcgcattc
    18781 aatcacgttg ttccggtact taatctgt
//
