LOCUS       NZ_CP116483            24575 bp    DNA     circular CON 27-FEB-2024
DEFINITION  Escherichia coli strain GTEN_24 plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP116483
VERSION     NZ_CP116483.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11230615
            Assembly: GCF_028215995.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24575)
  AUTHORS   Yorki,S., Shea,T., Worby,C.J., Cuomo,C., LaRocque,R.C., Manson,A.L.
            and Earl,A.M.
  TITLE     Comparison of long and short read metagenomic assembly for
            low-abundance species and resistance genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 24575)
  AUTHORS   Yorki,S., LaRocque,R.C., Worby,C.J., Shea,T., Earl,A.M.,
            Walker,B.J., Manson,A.L., Sridhar,S., Turbett,S., Becker,M.,
            Oliver,E., Bronson,R., Leung,D., Knouse,M., Hagmann,S., Kelly,P.,
            Harris,J. and Ryan,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2023) Infectious Diseases & Microbiome, Broad
            Institute of MIT and Harvard, 75 Ames St., Cambridge, MA 02142, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/27/2024 02:21:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,977
            CDSs (total)                      :: 4,853
            Genes (coding)                    :: 4,676
            CDSs (with protein)               :: 4,676
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 177
            CDSs (without protein)            :: 177
            Pseudo Genes (ambiguous residues) :: 0 of 177
            Pseudo Genes (frameshifted)       :: 69 of 177
            Pseudo Genes (incomplete)         :: 118 of 177
            Pseudo Genes (internal stop)      :: 28 of 177
            Pseudo Genes (multiple problems)  :: 33 of 177
            Pseudo Genes (short protein)      :: 2 of 177
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116483.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..24575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="GTEN_24"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: Massachusetts"
                     /collection_date="2018"
                     /collected_by="Massachusetts General Hospital"
     gene            join(24103..24575,1..190)
                     /locus_tag="E5Q65_RS24505"
     CDS             join(24103..24575,1..190)
                     /locus_tag="E5Q65_RS24505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_028917501.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_229038556.1"
                     /translation="MIEENPSFGYRTVAALLGFNKNTVQRIFQLKGWQVRKRPVGFRPR
                     IQALPSVASAPNQRWATDLCRIWAGRDGWCSLALVIDCHTRELLGWHLSRSGKSKTAEA
                     ALEQALIARFGSLGRVASPFLLRSDNGLVFTSRNYTALVKSYGLQQEFITPYTPEQNGM
                     VERVIRTLKEQCAHRQRFETLQHASRVIGDWIQFYNQKRPHQALKMKTPAQAYAEAA"
                     /name="IS3 family transposase"
     gene            complement(469..1173)
                     /locus_tag="E5Q65_RS24510"
     CDS             complement(469..1173)
                     /locus_tag="E5Q65_RS24510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067858.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            <1234..1314
                     /locus_tag="E5Q65_RS24515"
                     /pseudo=""
     CDS             <1234..1314
                     /locus_tag="E5Q65_RS24515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229638.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /name="MerR family transcriptional regulator"
     gene            complement(1321..2391)
                     /locus_tag="E5Q65_RS24520"
     CDS             complement(1321..2391)
                     /locus_tag="E5Q65_RS24520"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_272056254.1"
                     /translation="MLKTSGVNRDTARKQISRAASAGQIHCVDKLFPKRERFIYLKQEY
                     GTGRFWSSLNAALLDTGSAYGLALSCLRARGGILPVRNFSAACGSPVAMKNRLSWKSVL
                     DGLLQYKMVRVVTLPGLGECVALTEKNDNGYLRALHPLKARLLTESVLMKSLSQWVRNN
                     GIISYDTLRTREELNSDQTPCVANFDFDVTAASYLNPLLQFSRSGEIRPGFFVCDMLLG
                     CKLSLVHLQPFITKCRSINSLRNSPRCLFMFIADEYSEEAFLEMKRAGIIPATPENLFG
                     KDFADALFQLRDLVGSITLSLKDNIAAIDDIMSKLANIAGATNQLQGDLFEYIVAETVR
                     IDSKDVEVGGTSENGK"
                     /name="hypothetical protein"
     gene            complement(2682..3440)
                     /locus_tag="E5Q65_RS24525"
     CDS             complement(2682..3440)
                     /locus_tag="E5Q65_RS24525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000494510.1"
                     /translation="MFTLDCSTRSQALLSLSSGFGCSVMELKKVLLSLDLEQIYETDHS
                     IMIDSRQYLREYVCRELGIPGEFTTAYWFHGTRTSADNTFENGLLALNQTESLVMDMLV
                     NLAPDAEVKEKLQAWNFHAGVPDHLFRTRTRDKMHWGPYGHLVREVHLHARKLWQHDYV
                     RLPELVEDVCNAYKKKYGQDLTGHYLEVLKPCIVCFRADIDYEKGALEAALSYAYTSVR
                     ELPPDSGAVFGIDRHGKSVSVDEIVNVEFI"
                     /name="hypothetical protein"
     gene            complement(3452..3943)
                     /locus_tag="E5Q65_RS24530"
     CDS             complement(3452..3943)
                     /locus_tag="E5Q65_RS24530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN family nuclease"
                     /protein_id="WP_000528930.1"
                     /translation="MGNYSDFETDFIERTLALIDQYNNMIEGKPFPEQYNYTLTLNCLL
                     GLIVMPRERAVSYLPSDRLTPELKAEIGLNESQLPGEEMNLRELIHKMRNSVAHFCVQV
                     ESISDARLVDQIIFKETHGAGRAYAIFSAPELLPFLKYYAALLIANMRRHRGVPTTDVV
                     "
                     /name="HEPN family nuclease"
     gene            4217..4456
                     /locus_tag="E5Q65_RS24535"
     CDS             4217..4456
                     /locus_tag="E5Q65_RS24535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000371884.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSQGAIYLCESAKAGAVL
                     IFWHELAINGYASMNAIKRQELIDADFQRLRNGV"
                     /name="hypothetical protein"
     gene            complement(4646..6718)
                     /locus_tag="E5Q65_RS24540"
     CDS             complement(4646..6718)
                     /locus_tag="E5Q65_RS24540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="WP_001084041.1"
                     /translation="MNSFPIEQGEQLRVGTVDFVSPNEIRALLDIDSPDSVALNAGTPR
                     NFPRVNSYVLVSCDNGYLVGQIEWLAVEHSPYPKQRDIQDFGLVNLPFPRKKISLNPVG
                     NLKRFSKDGTDYFIFQRGSESFPSIGSAILLPTDLQLRSIVESGNNRRVIIGQSPLANN
                     ANVAVDPDRLFGRHIAVLGNTGSGKSCSVAGLIQWSLEAAMESEIKPNARFIILDPNGE
                     YTRALGPTTKFKGRVFKVEAEDGENQLQVPSWFWNSSEWASFTQASPKAQLPLLKRSLR
                     AMRNEEFDLQTNLDVEVKKYLGTILVSLKADKSKGAAALNDFPGAKNLLAKINTWRQSL
                     EEYKERLVTTHPELDKLIVSIQDFCVQREGRYPDYNAKVSAVDNIIDGMLSSFQSLGGN
                     ECELLPKNEDIPVPFDGGNLVSYLEALAQENGSEQYVEYLVARIRTMLADTRMKPITND
                     SEHRVDLANWLETYIGKDGTGDDDSCVSIIDLSLVPTEITHLVTAVISRIVFESLQRYR
                     RLYNKSLPTVLVAEEAHTFIKRYRDDSENQDVAAVCCQVFEKIAREGRKFGLGMVISSQ
                     RPSELSPTVLSQCNTFLLHRISNDKDQEQVHKMVPDNLRGLLRELPSLPSQHAILMGWA
                     SELPVLVKMKNLTKEQQPHSDDPDFWDVWTRKYADGKLVERTVDWEAVVKEWQQK"
                     /name="DUF87 domain-containing protein"
     gene            complement(6715..8106)
                     /locus_tag="E5Q65_RS24545"
     CDS             complement(6715..8106)
                     /locus_tag="E5Q65_RS24545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="WP_000085084.1"
                     /translation="MSQYLKLGDDQSPVQLDPHSEVGAFKVVGHCAWAKPEDKITPDFL
                     RSRVEPWLTALFQSEHLNILIGAGLSSAIQESATGTKPQGMGWIEDLKVCKAEIDSYVA
                     KTADASGRGKGNIEDQIRSINELIKGLEILTVQNLPVPLPPPGAPPYRNLKSELVELNN
                     ELTRCLKLFSDSVSHGEKLIRDASNDLKTETFNYLVSFLMSFSSRTATRDRLHIFTTNY
                     DRIIETGAEIAGLRLIDRFVGTIAPIFRSSRLEVDYHYNPPGIRGEPRYLEGVARFTKL
                     HGSLDWYATEGAIRRFGLPFGASSVEPFLQVETAGTANYQQLMIYPNSVKDRETSEYPY
                     VELFRDLASATCRPNSTLVTYGYSFGDEHINRLIIDMLTIPSTHIVIIAYGDPLGRIMR
                     FINDSGRKAQISLLLGDHFGDLKKLVDFYLPKAAIDRSSIRMAELLKSRGLYRATKPNN
                     SEDAE"
                     /name="SIR2 family protein"
     gene            complement(8183..8707)
                     /locus_tag="E5Q65_RS24550"
     CDS             complement(8183..8707)
                     /locus_tag="E5Q65_RS24550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196200.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_050195267.1"
                     /translation="MISLSPPTICNSAVFTDMMSGATDEREGLQRLLARAEKDDIILCT
                     KMDRLGRNTADMIRIVDTCYKKGIAIRFLENGLSTEGTMGKMVIQILAAVAEAERERIL
                     ERTNDGRQAAMIKGVKFGRKPHKKAELANELINKDVQVKEVMEKTGISRATYFRLKRKT
                     VTNNIHLSNNK"
                     /name="recombinase family protein"
     gene            8732..9436
                     /locus_tag="E5Q65_RS24555"
     CDS             8732..9436
                     /locus_tag="E5Q65_RS24555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            <9491..9778
                     /locus_tag="E5Q65_RS24560"
                     /pseudo=""
     CDS             <9491..9778
                     /locus_tag="E5Q65_RS24560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            10034..10909
                     /locus_tag="E5Q65_RS24565"
     CDS             10034..10909
                     /locus_tag="E5Q65_RS24565"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class A beta-lactamase
                     CTX-M-55"
                     /protein_id="WP_015387340.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQSG
                     GRLGVALINTADNSQILYRADERFAMCSTSKVMAVAAVLKKSESEPNLLNQRVEIKKSD
                     LVNYNPIAEKHVNGTMSLAELSAAALQYSDNVAMNKLIAHVGGPASVTAFARQLGDETF
                     RLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNTTGAA
                     SIQAGLPASWVVGDKTGSGGYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESRRDVLA
                     SAAKIVTDGL"
                     /name="extended-spectrum class A beta-lactamase CTX-M-55"
     gene            complement(10956..>11300)
                     /locus_tag="E5Q65_RS24570"
                     /pseudo=""
     CDS             complement(10956..>11300)
                     /locus_tag="E5Q65_RS24570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016943399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /name="WbuC family cupin fold metalloprotein"
     gene            <11314..>14064
                     /locus_tag="E5Q65_RS24575"
                     /pseudo=""
     CDS             <11314..>14064
                     /locus_tag="E5Q65_RS24575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /name="Tn3-like element Tn3 family transposase"
     gene            complement(<14140..15155)
                     /locus_tag="E5Q65_RS24580"
                     /pseudo=""
     CDS             complement(<14140..15155)
                     /locus_tag="E5Q65_RS24580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            15550..16206
                     /locus_tag="E5Q65_RS24585"
     CDS             15550..16206
                     /locus_tag="E5Q65_RS24585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrS1"
                     /protein_id="WP_001516695.1"
                     /translation="METYNHTYRHHNFSHKDLSDLTFTACTFIRSDFRRANLRDTTFVN
                     CKFIEQGDIEGCHFDVADLRDASFQQCQLAMANFSNANCYGIEFRACDLKGANFSRTNF
                     AHQVSNRMYFCSAFISGCNLSYANMERVCLEKCELFENRWIGTNLAGASLKESDLSRGV
                     FSEDVWGQFSLQGANLCHAELDGLDPRKVDTSGIKIAAWQQELILEALGIVVYPD"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrS1"
     gene            complement(16525..>16839)
                     /locus_tag="E5Q65_RS24590"
                     /pseudo=""
     CDS             complement(16525..>16839)
                     /locus_tag="E5Q65_RS24590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017412822.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /name="recombinase family protein"
     gene            16905..17609
                     /locus_tag="E5Q65_RS24595"
     CDS             16905..17609
                     /locus_tag="E5Q65_RS24595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067858.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(<17662..18177)
                     /locus_tag="E5Q65_RS24600"
                     /pseudo=""
     CDS             complement(<17662..18177)
                     /locus_tag="E5Q65_RS24600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /name="IS4-like element ISVsa5 family transposase"
     gene            18320..19180
                     /gene="aac(3)-IId"
                     /locus_tag="E5Q65_RS24605"
     ARG             18321..19177
                     /name="aac(3)-IId"
                     /gene="aac(3)-IId"
                     /gene_name="aminoglycoside N-acetyltransferase AAC(3)-IId"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            19193..19735
                     /locus_tag="E5Q65_RS24610"
     CDS             19193..19735
                     /locus_tag="E5Q65_RS24610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000587837.1"
                     /translation="MIIWINGPFGAGKTTLAKRLRDRRSKSLIFDPEEIGFVVKETVPM
                     PASGDYQDLPLWRGLTIAAVREIRRNYSQDIIIPMTLVHPDYLTEILDGVRRIDDQLLH
                     IFLTLNEDLLRHRIANQTMHPDPNRNAEIREWRLANVARCLAARERLPCTTRVLDSGAH
                     TSDELAAMVLDGIDGRT"
                     /name="AAA family ATPase"
     gene            complement(19878..>20252)
                     /locus_tag="E5Q65_RS24615"
                     /pseudo=""
     CDS             complement(19878..>20252)
                     /locus_tag="E5Q65_RS24615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(20287..>21141)
                     /locus_tag="E5Q65_RS24620"
                     /pseudo=""
     CDS             complement(20287..>21141)
                     /locus_tag="E5Q65_RS24620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021138877.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /name="Tn3 family transposase"
     gene            21315..22940
                     /locus_tag="E5Q65_RS24625"
     CDS             21315..22940
                     /locus_tag="E5Q65_RS24625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005302376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase MCR-3.1"
                     /protein_id="WP_039026394.1"
                     /translation="MPSLIKIKIVPLMFFLALYFAFMLNWRGVLHFYEILYKLEDFKFG
                     FAISLPILLVAALNFVFVPFSIRYLIKPFFALLIALSAIVSYTMMKYRVLFDQNMIQNI
                     FETNQNEALAYLSLPIIVWVTIAGFIPAILLFFVEIEYEEKWFKGILTRALSMFASLIV
                     IAVIAALYYQDYVSVGRNNSNLQREIVPANFVNSTVKYVYNRYLAEPIPFTTLGDDAKR
                     DTNQSKPTLMFLVVGETARGKNFSMNGYEKDTNPFTSKSGGVISFNDVRSCGTATAVSV
                     PCMFSNMGRKEFDDNRARNSEGLLDVLQKTGISIFWKENDGGCKGVCDRVPNIEIEPKD
                     HPKFCDKNTCYDEVVLQDLDSEIAQMKGDKLVGFHLIGSHGPTYYKRYPDAHRQFTPDC
                     PRSDIENCTDEELTNTYDNTIRYTDFVIGEMIAKLKTYEDKYNTALLYVSDHGESLGAL
                     GLYLHGTPYQFAPDDQTRVPMQVWMSPGFTKEKGVDMACLQQKAADTRYSHDNIFSSVL
                     GIWDVKTSVYEKGLDIFSQCRNVQ"
                     /name="phosphoethanolamine--lipid A transferase MCR-3.1"
     gene            23058..23438
                     /locus_tag="E5Q65_RS24630"
     CDS             23058..23438
                     /locus_tag="E5Q65_RS24630"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017410875.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0004143 - ATP-dependent diacylglycerol kinase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]; GO:0008654 - phospholipid
                     biosynthetic process [Evidence IEA]; GO:0016310 -
                     phosphorylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="WP_039026395.1"
                     /translation="MAKPGATGITRIIHATNYSMKGLSSAWRHEAAFRQELLLIVLLMP
                     LAFFVGDGLNQILLLMVISWLVVIVEILNSAIEAVVDRIGSEHHELSGRAKDMGSAAVF
                     IALMLNAIVWGALTGRNLLTWW"
                     /name="diacylglycerol kinase"
     gene            23633..23947
                     /locus_tag="E5Q65_RS24635"
     CDS             23633..23947
                     /locus_tag="E5Q65_RS24635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816713.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_039026396.1"
                     /translation="MSITMDEEIKRWTAKRKSALVMEIIQGKTTVAEAARAFDLPPSEI
                     EEWVDDAKRGMENALRAKPLDIREQYEKQLKELQEAYGEAMLELRARKKLASLLGEDDR
                     "
                     /name="transposase"
     ARG             15551..16203
                     /name="qnrS1"
                     /gene="qnrS1"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrS1"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             21316..22937
                     /name="mcr-3.1"
                     /gene="mcr-3.1"
                     /gene_name="phosphoethanolamine--lipid A transferase
                     MCR-3.1"
                     /tool="amrfinderplus"
                     /drug_class="COLISTIN"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             10035..10906
                     /name="blaCTX-M-55"
                     /gene="blaCTX-M-55"
                     /gene_name="extended-spectrum class A beta-lactamase
                     CTX-M-55"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 ggaacaaaac ggcatggtcg agcgggtgat cagaacgctc aaggagcagt gtgcccatcg
       61 gcagaggttc gagaccttgc agcatgccag tcgggtgatt ggcgactgga tccagtttta
      121 caaccagaag cgtcctcacc aggcgctaaa gatgaaaaca ccggcgcagg cctatgccga
      181 agccgcttag ttgtgcagga acggctgggt cattacagtt tatcgcaata aaaacctata
      241 ctctttttaa tatccccgac tggcaatgcc gggatagact attattctcc ccggagccac
      301 tacgctgacc cggttgattt cagaggtaag ggaaaaggcg acgttgcgcc tgtggaacaa
      361 actggcactg ataccgtcag ccgaacagcg ttcacagctg gagatgctgc tggggcggca
      421 ctgttgcaaa gttagcgatg aggcagcctt ttgtcttatt caaaggcctt acatttcaaa
      481 aactctgctt accaggcgca tttcgcccag gggatcacca taataaaatg ctgaggcctg
      541 gcctttgcgt agtgcacgca tcacctcaat acctttgatg gtggcgtaag ccgtcttcat
      601 ggatttaaat cccagcgtgg cgttgattat ccgtttcagt ttgccatgat cgcattcaat
      661 cacgttgttc cggtacttaa tctgtcggtg ttcaacgtca gacgggcacc ggccttcgcg
      721 tttgagcaga gcaagcgcgc gaccataggc gggcgcttta tccgtgttga tgaatcgtgg
      781 gatctgccac ttcttcacgt tgttgaggat tttacccaga aaccggtatg cagctttgct
      841 gttacgacgg gaggagagat aaaaatcgac agtgcggccc cggctgtcga cggcccggta
      901 cagatacgcc cagcggccat tgaccttcac gtaggtttca tccatgtgcc acgggcaaag
      961 atcggaaggg ttacgccagt accagcgcag ccgtttttcc atttcaggcg cataacgctg
     1021 aacccagcgg taaatcgtgg agtgatcgac attcactccg cgttcagcca gcatctcctg
     1081 cagctcacgg taactgatgc cgtatttgca gtaccagcgt acggcccaca gaatgatgtc
     1141 acgctgaaaa tgccggcctt tgaatgggtt catgtgcagc tccatcagca aaaggggatg
     1201 ataagtttat caccaccgac tatttgcaac agtgcccgaa aggggaatgt ttcctgcccg
     1261 ttgatcgcgt cactacaggg cgaagcaggc ctggcaaggt cagctatgcc ttagcgtgct
     1321 ttattttccg ttttctgagg tgcccccaac ctcaacgtcc ttactgtcta tacgcacggt
     1381 ctcagcgaca atatattcaa ataaatcgcc ctgaagctga ttagtcgcgc cggctatatt
     1441 cgccagcttg gacatgatgt catctattgc ggctatatta tcttttaagc tgagcgttat
     1501 ggagccgaca agatcccgta gctgaaagag tgcatcagca aagtctttcc cgaagagatt
     1561 ttccggcgtg gcgggtatta tcccggcacg cttcatttcc agaaatgcct cttcactgta
     1621 ttcatcagcg atgaacataa acaggcatct ggggctgttc cggaggctgt ttatcgagcg
     1681 acatttcgta ataaaaggct ggagatgcac cagactgagc ttacagccca agagcatgtc
     1741 gcagacaaag aaaccaggcc ggatttcacc tgaccgggag aactgcagaa gaggattaag
     1801 ataggatgca gcagtgacgt caaaatcaaa atttgccacg cagggagtct gatccgaatt
     1861 gagctcctct cgcgtacgca gcgtgtcgta actgatgatg ccattgttac ggacccactg
     1921 actcagggat ttcatcagaa ctgactcggt gagtagtctg gctttcaggg gatggagcgc
     1981 gcgcagataa ccgttatcat ttttttctgt cagcgcaaca cattccccca gacccggtaa
     2041 cgtaacgacc ctgaccattt tgtactgaag cagaccgtcc agaactgact tccaggataa
     2101 tctgtttttc attgctaccg gcgaaccgca ggctgcagaa aaatttctga ccggcaggat
     2161 ccccccgcgt gcccggaggc aactcagggc gagaccgtag gccgacccgg tatcaagaag
     2221 ggccgcattg agactggacc agaagcgccc ggtaccatac tcttgtttca gataaataaa
     2281 tcgttcgcgc ttggggaaaa gcttgtcaac acagtggatt tgccctgcag aagcagcccg
     2341 ggaaatctgt ttgcgcgcgg tatcacggtt aacgcccgag gttttcagca tttcctctac
     2401 aagtacagag ctaaggcaag gaccggtttt gagtaacgaa gtaactataa tcgatgtcat
     2461 cttttgctca cttgtcacac tgcgtattta ataataacaa tacactcaag catcgaaacg
     2521 acaaagcctg cagtttagac ctgtaaatac aataggttaa atgacataaa tgaggccctt
     2581 tagattatct gtcacaaact tcattttcct gtagaaaatt ttgccactaa aaaaagaagt
     2641 attgataaca tcaataaccc tacgggcttc agtcatcaag ctcatatgaa ttcaacattc
     2701 acaatttcat cgacagaaac ggatttacca tgtcgatcaa taccgaacac agcgccacta
     2761 tccggtggca attctctaac agacgtgtat gcgtaactaa gcgcagcttc aagcgcaccc
     2821 ttttcataat caatatccgc cctgaaacag actatgcaag gtttcagtac ctcaagataa
     2881 tgtcctgtaa gatcctgccc atattttttt ttgtaagcat tgcagacatc ctctaccagc
     2941 tccggcagcc gtacatagtc gtgctgccag agtttacgtg catgaagatg gacctcccgc
     3001 acaaggtgac catatggtcc ccagtgcatt ttatccctgg ttcgggtccg aaacaggtga
     3061 tcaggtacgc ctgcatgaaa attccaggcc tgtaatttct ctttgacctc agcgtctgga
     3121 gccagattta ccagcatgtc catcaccagt gactcagttt ggttcagtgc aagtaaacca
     3181 ttttcaaaag tattatcagc agaagtgcgg gtaccatgaa accagtatgc ggtggtgaac
     3241 tcacccggaa tacccagttc cctacatacg tactctctca gatactgtcg tgaatcgatc
     3301 ataatggaat gatccgtctc gtaaatctgc tcaagatcga gagataacaa aacctttttt
     3361 agctccatta cactacaacc aaaaccggat gaaagtgaaa gcagcgcctg agatcgtgtt
     3421 gaacaatcga gagtaaacat ggttgcattc cttacacaac gtcagtcgtg gggacacccc
     3481 gatggcgtct catattggcg atcaacaaag ccgcgtaata ttttagaaat gggagaagct
     3541 ccggggcact gaaaattgca taagcccggc cagcaccatg cgtctctttg aagatgattt
     3601 gatcaactag gcgtgcatcg ctgatagatt caacctgaac acagaaatgc gccacagaat
     3661 ttcgcatttt atgaatcagt tctcttaagt tcatttcctc acccggtaac tgcgactcgt
     3721 ttaacccaat ttcagcttta agctctggcg ttagacgatc cgacgggagg taagaaacag
     3781 cgcgctctct cggcatcacg attaggccca aaagacagtt cagagtaagc gtatagttgt
     3841 actgctcagg gaagggcttc ccctcaatca tattgttata ttgatcgatt agggccagtg
     3901 ttctttcgat aaaatcagtc tcaaagtctg aatagtttcc cactaaatcc tccttcggaa
     3961 gcagaaaatc taactatacc attcagatat ccctacggtt catatcctta ctttccgatt
     4021 ttgccggaaa ccggatctgc gcggcagaaa agacgcaaat gatatgataa ttaagaaata
     4081 tacataaaat catatcagat atgattttca tcattaaaaa ttaaacaata tctgatatta
     4141 tcagaaatca tgagcacaca aatcatgcag gatatgattt ttacctgtca gaacattatc
     4201 agatggagtg tgcaggatgg atcaggaaat gacgttttca ctcagctatg aacaactgac
     4261 ccggtttgcg gaaaaacgca tccgggaatg caatctggac agccagggcg cgatttatct
     4321 ctgcgagtca gccaaagccg gagccgtgct gatcttctgg catgaactgg ccataaatgg
     4381 ctatgcctca atgaatgcca taaagagaca ggagctcatt gacgccgact ttcagcgcct
     4441 cagaaacggg gtttgagggc caatgatgca aaaaaccacg ctaagaggtt agttcattgt
     4501 ttatattgag attgttaggt actgcgtttg cagccacttc agtcgtcatg gggggaggga
     4561 caatggaacg caacaccaag tagattgatg gcataaagtt ccttcaattc cgatctgctc
     4621 tcccgctttc attttctgct gcagatcact tttgttgcca ttcctttaca acagcttccc
     4681 aatcgacagt tctctcgacc aacttcccat ccgcatactt tcttgtccaa acatcccaga
     4741 agtcagggtc atcagaatga ggttgctgct ctttcgtcaa atttttcatt ttaactagga
     4801 caggaagttc agatgcccag cccatcagta ttgcatgttg ggatggtaat gaaggtagtt
     4861 cacggagtaa tcctcgcaaa ttatcaggta ccattttatg aacttgctcc tgatctttgt
     4921 cgttactaat tcgatgcaaa agaaaagtat tacattgaga caaaaccgta ggtgataact
     4981 ctgatggtcg ttgtgaggaa atcaccatac caagaccaaa ctttctgcct tctcgtgcga
     5041 ttttctcaaa cacttggcag caaacggctg cgacatcctg attttcagag tcatcacgat
     5101 accgtttaat aaatgtatgt gcttcttcag cgacaagtac cgtaggtaaa gatttattat
     5161 atagccgcct ataacgttgc aatgattcaa acacaatcct tgaaataaca gctgtcacaa
     5221 gatgagtaat ttctgttggt actaaagaaa gatcaataat tgaaacacag ctatcatcat
     5281 cgccggttcc atcctttcca atataagttt ctaaccaatt ggctaagtca actcggtgct
     5341 ctgagtcatt ggttataggt ttcattctcg tatcagcaag cattgtgcgt atcctagcca
     5401 ctaaatattc gacatattgc tcactgccat tttcctgggc taatgcctct aaataggaaa
     5461 ctaaattacc tccgtcaaaa gggacgggga tgtcctcatt tttcggaagg agttcacact
     5521 cattaccacc aaggctctga aatgaagata acattccatc tatgatgtta tcaactgcgc
     5581 taactttagc attgtaatct ggatatctac cctctctttg cacacaaaaa tcttgaatag
     5641 agacgattaa tttatctagc tctggatgtg tagtaactaa cctttcttta tattcttcaa
     5701 gactctgacg ccaagtgtta atttttgcca aaagattttt agcacctggg aaatcattaa
     5761 gtgctgctgc acctttagat ttatccgctt ttaaagaaac taaaatagta cctaagtact
     5821 ttttaacttc tacatctaaa ttcgtttgta aatcaaactc ttcatttctc attgctctca
     5881 aagagcgctt tagtaatgga agttgtgctt taggacttgc ttgcgtgaat gatgcccatt
     5941 ctgaactatt ccaaaaccat gaaggtacct gtaattggtt ttcaccatct tctgcttcaa
     6001 ccttaaacac tctgccttta aattttgtag ttggccccaa agcacgtgta tactcgccat
     6061 ttgggtctaa tataataaat cttgcgtttg gttttatttc tgattccatt gcagcctcga
     6121 gtgaccactg gattaaccct gcaacagaac aagacttacc actacccgta ttaccaagta
     6181 ctgctatatg acgaccaaat aatctgtcgg gatctacagc cacattagca ttgttagcaa
     6241 gtgggctttg cccaataatc acccgcctat tattaccaga ctcgacaatt gatctaagct
     6301 gtaaatctgt tggtagtaaa atagctgaac caatagaagg aaatgattca gaaccgcgtt
     6361 gaaatataaa ataatcagta ccatcttttg aaaaacgttt aagattccct accggattta
     6421 aacttatttt ttttcgaggg aatggcagat ttactaaacc aaaatcttgg atatcccttt
     6481 gtttgggata aggcgaatgc tcaactgcaa gccactctat ttgcccaact aagtaaccat
     6541 tgtcacatga gactaataca tagctgttta ctctaggaaa atttctaggt gttcctgcat
     6601 tcaaagcgac agaatcaggc gagtcgatat ctaatagtgc cctaatctcg ttgggtgaaa
     6661 caaaatcgac agtgccaacc ctgagttgct caccttgttc aatggggaat gagttcattc
     6721 agcatcctca ctattatttg gttttgttgc acgatataaa cctcgtgatt ttaatagctc
     6781 agccatacga attgatgatc tgtctatcgc ggctttagga agataaaaat caactaattt
     6841 ttttagatct ccaaaatgat ctcccagcaa taaagatatt tgagcttttc ttccactatc
     6901 atttataaaa cgcataattc ttccaagcgg atcaccgtaa gcaattatga ctatatgcgt
     6961 tgaggggatt gtcaacatat caattataag ccgatttata tgctcatctc caaaactata
     7021 accatatgtt accaaggtgc tattaggacg acatgttgca gatgctaaat ccctaaatag
     7081 ctcaacataa ggatattcag atgtttcacg gtctttaact gaatttggat aaatcattaa
     7141 ttgttgataa tttgcagttc cggctgtttc cacttgtaaa aagggttcaa ccgagctagc
     7201 tccaaatggc aatccaaaac gccttatagc cccttcagta gcataccagt ccaatgaacc
     7261 atgtaattta gtaaagcgcg ccacaccttc caaatatctt ggttcacccc gaatacctgg
     7321 tggattgtaa tgataatcca cttctaatcg cgaagatcta aaaatcggcg ctatggtacc
     7381 gacaaatctg tcaattaaac gaagacctgc tatttcagcg cccgtttcaa ttattctgtc
     7441 gtaatttgtc gtaaaaatat gtaatcgatc cctagttgct gttcgacttg aaaaactcat
     7501 caaaaaactc actaaataat taaacgtttc tgtttttaaa tcattgcttg catcacgaat
     7561 taatttttcc ccatgactaa ctgagtccga aaataatttc agacatcttg ttagctcatt
     7621 atttaactct actaactcac tttttaaatt tcgatatggg ggagcacctg gtggaggtag
     7681 tggaaccggt aggttttgta ccgttaaaat ctctaatcct ttaatcaact cattaattga
     7741 acgaatctga tcttcaatat taccctttcc ccttcctgag gcgtctgctg ttttagctac
     7801 ataactgtct atttcagcct tacaaacttt gagatcttca atccatccca taccttgagg
     7861 tttggtgccg gtcgctgact cttgaatagc cgaactaagg ccagcgccaa taagtatatt
     7921 taagtgttca gactgaaaaa gggcagttaa ccaaggttct actcttgaac gcaaaaagtc
     7981 aggagtgatt ttatcttctg gttttgccca tgcgcagtgt ccaacgactt taaatgcacc
     8041 aacttcgctg tgtgggtcca actgaaccgg agattggtca tctccaagct ttaaatattg
     8101 actcaatttg aatacatcct tttttcgagt ttaaacattg ctaaatagaa gccgttcagg
     8161 catttaagaa tgctcatctt tcttatttat tattggaaag atgaatattg ttagtaaccg
     8221 tttttctttt cagcctaaaa tacgtcgccc gtgatatacc cgttttttcc attacctctt
     8281 taacttgtac gtccttattg atcaattcat tagctaattc agccttttta tgaggctttc
     8341 ttccaaattt gacgccctta atcatagctg cctgccgccc atcgttagtt cgctccaaga
     8401 tcctttcacg ttccgcctcg gcaacagctg ctaatatctg gataaccatt tttcccatag
     8461 tgccttcagt actaagcccg ttctcaagaa agcgaatagc gatgcctttt ttatagcaag
     8521 tatccacgat gcgaatcata tctgcggtat tacgtccaag gcgatccatt ttagtacaga
     8581 gaataatgtc atctttctcc gctctggcaa ggagtcgctg tagcccttcg cgctcatcag
     8641 ttgctcccga catcatgtca gtaaacacgg cactgttgca aatagtcggt ggtgataaac
     8701 ttatcatccc cttttgctga tggagctgca catgaaccca ttcaaaggcc ggcattttca
     8761 gcgtgacatc attctgtggg ccgtacgctg gtactgcaaa tacggcatca gttaccgtga
     8821 gctgcaggag atgctggctg aacgcggagt gaatgtcgat cactccacga tttaccgctg
     8881 ggttcagcgt tatgcgcctg aaatggaaaa acggctgcgc tggtactggc gtaacccttc
     8941 cgatctttgc ccgtggcaca tggatgaaac ctacgtgaag gtcaatggcc gctgggcgta
     9001 tctgtaccgg gccgtcgaca gccggggccg cactgtcgat ttttatctct cctcccgtcg
     9061 taacagcaaa gctgcatacc ggtttctggg taaaatcctc aacaacgtga agaagtggca
     9121 gatcccgcga ttcatcaaca cggataaagc gcccgcctat ggtcgcgcgc ttgctctgct
     9181 caaacgcgaa ggccggtgcc cgtctgacgt tgaacaccga cagattaagt accggaacaa
     9241 cgtgattgaa tgcgatcatg gcaaactgaa acggataatc ggcgccacgc tgggatttaa
     9301 atccatgaag acggcttacg ccaccatcaa aggtattgag gtgatgcgtg cactacgcaa
     9361 aggccaggcc tcagcatttt attatggtga tcccctgggc gaaatgcgcc tggtaagcag
     9421 agtttttgaa atgtaaggcc tttgaataag acaaaaggct gcctcatcgc taactttgca
     9481 acagtgccct gaaaactata tcaaagaagc caaatacgac atggcggtgg gtcatctctt
     9541 gctaaagtca ttttgggcga atgaagccgt gtttcaaatg atgatgcttt catataacct
     9601 atttttgttg ttcaagtttg attccttgga ctcttcagaa tacagacagc aaataaagac
     9661 ctttcgtttg aagtatgtat ttcttgcagc aaaaataatc aaaaccgcaa gatatgtaat
     9721 catgaagttg tcggaaaact atccgtacaa gggagtgtat gaaaaatgtc tggtataata
     9781 agaatatcat caataaaatt gagtgttgct ctgtggataa cttgcagagt ttattaagta
     9841 tcattgcagc aaagatgaaa tcaatgattt atcaaaaatg attgaaaggt ggttgtaaat
     9901 aatgttacaa tgtgtgagaa gcagtctaaa ttcttcgtga aatagtgatt tttgaagcta
     9961 ataaaaaaca cacgtggaat ttagggacta ttcatgttgt tgttatttcg tatcttccag
    10021 aataaggaat cccatggtta aaaaatcact gcgccagttc acgctgatgg cgacggcaac
    10081 cgtcacgctg ttgttaggaa gtgtgccgct gtatgcgcaa acggcggacg tacagcaaaa
    10141 acttgccgaa ttagagcggc agtcgggagg cagactgggt gtggcattga ttaacacagc
    10201 agataattcg caaatacttt atcgtgctga tgagcgcttt gcgatgtgca gcaccagtaa
    10261 agtgatggcc gtggccgcgg tgctgaagaa aagtgaaagc gaaccgaatc tgttaaatca
    10321 gcgagttgag atcaaaaaat ctgaccttgt taactataat ccgattgcgg aaaagcacgt
    10381 caatgggacg atgtcactgg ctgagcttag cgcggccgcg ctacagtaca gcgataacgt
    10441 ggcgatgaat aagctgattg ctcacgttgg cggcccggct agcgtcaccg cgttcgcccg
    10501 acagctggga gacgaaacgt tccgtctcga ccgtaccgag ccgacgttaa acaccgccat
    10561 tccgggcgat ccgcgtgata ccacttcacc tcgggcaatg gcgcaaactc tgcggaatct
    10621 gacgctgggt aaagcattgg gcgacagcca acgggcgcag ctggtgacat ggatgaaagg
    10681 caataccacc ggtgcagcga gcattcaggc tggactgcct gcttcctggg ttgtggggga
    10741 taaaaccggc agcggtggct atggcaccac caacgatatc gcggtgatct ggccaaaaga
    10801 tcgtgcgccg ctgattctgg tcacttactt cacccagcct caacctaagg cagaaagccg
    10861 tcgcgatgta ttagcgtcgg cggctaaaat cgtcaccgac ggtttgtaat agcggaaacg
    10921 gaatggggaa actcattccg tttttgttta tcgccttaga cggcaaaagt gctgtcgccc
    10981 acctgcgctt gcgcatacca ggccataagc tccgtggttc ctggttctcc ttccgctgga
    11041 gcccagtgcg catagtcatc ggcagccacg ggttgatagc caccgtgttt tacttcaaaa
    11101 attatgccac cggtatccag cgacagcacg gcatgccagg ttcctgcggc catctccagc
    11161 accgtacagg tttcccccaa tatcgcccga tgggtgacgg taccccgatc gtcaaaattc
    11221 agcaccacga aacgacccct taatggcaac agtagctcga aggtgtgagg gtgtcggtgc
    11281 gggcgcacgt aggtcccagg tcatattcct tccggcgtcc ggcattttac cgccagacag
    11341 ctcgggattc gtgatatcac cgttcttgca gaatacggtc agaggaaaaa tacccgccgt
    11401 gagcatgcag cgctgatacg tcagcactat cagtatcgtg aatttgcctg gccctggaca
    11461 tttcgcctta cccgtctttt atatacccgg agctggataa gcaacgaacg tcctggcctg
    11521 cttttcgatc tggcgacagg gtggcttatg caacatcgta ttattctccc cggagccact
    11581 acgctgaccc ggttgatttc agaggtaagg gaaaaggcga cgttgcgcct gtggaacaaa
    11641 ctggcactga taccgtcagc cgaacagcgt tcacagctgg agatgctgct ggggccaact
    11701 gattgcagcc gcctgtcttt actggaatca ctgaaaaagg gccctgtgac catcagtggt
    11761 ccggcgttta atgaagcaat tgaacgctgg aaaactctga acgattttgg cctgcatgct
    11821 gaaaacctga gtacactccc ggctgtgcgc ctgaaaaatc tcgcacgtta tgctggtatg
    11881 acttcggtgt tcaatattgc caggatgtca ccgcagaaaa ggatggcggt tctggttgcc
    11941 tttgtccttg catgggaaac gctggcgctg gatgatgcat tggacgttct ggacgccatg
    12001 ctggccgtta tcatccgtga cgccagaaag attgggcaga aaaaacggct ccgctcgctg
    12061 aaggatctgg ataaatctgc attggcgctc gccagcgcat gttcgtacct gctgaaagaa
    12121 gaaacaccgg acgaatcgat tcgtgctgag gtgttcagct acatcccaag gcaaaagctg
    12181 gctgaaatca tcacgcttgt ccgtgaaatt gcccggccct cagacgataa ttttcatgaa
    12241 gaaatggtgg agcagtacgg gcgcgttcgt cgtttcctgc cccatctgct gaataccgtt
    12301 aaattttcat ccgcacctgc cggggttacc actctgaatg cctgtgacta cctcagccgg
    12361 gagttcagct cacggcggca gttttttgac gacgcaccaa cggaaattat cagtcggtca
    12421 tggaaacggc tggtgattaa caaggaaaaa catatcaccc gcaggggata cacgctctgc
    12481 tttctcagta aactgcagga tagtctgagg cggagggatg tctacgttac cggcagtaac
    12541 cggtggggag atcctcgtgc aagattacta cagggtgctg actggcaggc aaaccggatt
    12601 aaggtttatc gttctttggg gcacccgaca gacccgcagg aagcaataaa atctctgggt
    12661 catcagcttg atagtcgtta cagacaggtt gctgcacgtc tttgcgaaaa tgaggctgtc
    12721 gaactcgatg tttctggccc gaagccccgg ttgacaattt ctcccctcgc cagtcttgat
    12781 gagccggaca gtctgaaacg actgagcaaa atgatcagtg atctactccc tccggtggat
    12841 ttaacggagt tgctgctcga aattaacgcc cataccggat ttgctgatga gtttttccat
    12901 gctagtgaag ccagtgccag agttgatgat ctgcccgtca gcatcagcgc cgtgctgatg
    12961 gctgaagcct gcaatatcgg tctggaacca ctgatcagat caaatgttcc tgcactgacc
    13021 cgacaccggc tgaactggac aaaagcgaac tatctgcggg ctgaaactat caccagcgct
    13081 aatgccagac tggttgattt tcaggcaacg ctgccactgg cacagatatg gggtggagga
    13141 gaagtggcat ctgcagatgg aatgcgcttt gttacgccag tcagaacaat caatgccgga
    13201 ccgaaccgca aatactttgg taataacaga gggatcacct ggtacaactt tgtgtccgat
    13261 cagtattccg gctttcatgg catcgttata ccggggacgc tgagggactc tatctttgtg
    13321 ctggaaggtc ttctggaaca ggagaccggg ctgaatccaa ccgaaattat gaccgataca
    13381 gcaggtgcca gcgaacttgt ctttggcctt ttctggctgc tgggatacca gttttctcca
    13441 cgcctggctg atgccggtgc ttcggttttc tggcgaatgg accatgatgc cgactatggc
    13501 gtgctgaatg atattgccag agggcaatca gatccccgaa aaatagtcct tcagtgggac
    13561 gaaatgatcc ggaccgctgg ctccctgaag ctgggcaaag tacaggtttc agtgctggtc
    13621 cgttcattgc tgaaaagtga acgtccttcc ggactgactc aggcaatcat tgaagtgggg
    13681 cgcatcaaca aaacgctgta tctgcttaat tatattgatg atgaagatta ccgccggcgc
    13741 attctgaccc agcttaatcg gggagaaagt cgccatgccg ttgccagagc catctgtcac
    13801 ggtcaaaaag gtgagataag aaaacgatat accgacggtc aggaagatca actgggcaca
    13861 ctggggctgg tcactaacgc cgtcgtgtta tggaacacta tttatatgca ggcagccctg
    13921 gatcatctcc gggcgcaggg tgaaacactg aatgatgaag atatcgcacg cctctccccg
    13981 ctttgccacg gacatatcaa tatgctcggc cattattcct tcacgctggc agaactggtg
    14041 accaaaggac atctgagacc attaaaagag gcgtcagagg cagaaaacgt tgcttaacgt
    14101 gagttttcgt tccactgagc gtcagacccc taaaatcctg atgctgatgt agtcccgttt
    14161 tatcgtcatc acgaagcttt ctgctatgcc attgctttcc gggcttcgca ctgctgtcgt
    14221 acaaggctcc agtcccagca accgggcgaa cgccggcact gttggcaact gccttccgaa
    14281 gcgtttttcc accgcaccca gcatgacatc ctgcaccgtt tctttgtcat agcctccggt
    14341 gctcgctgcc cagtcaatga tctccctgtc gcagcagtcc tgcgcgaagg tcacccgcag
    14401 cttttcgcca ttatcacagc ggaactcaaa gccatctgag caccagcgcc ggttactttc
    14461 cgctactgct acgcgcccct tatgggcccg cttacggcaa ggatcagcgg gtttacgttc
    14521 aagaagcaga ttatgcgttc tcataatgcg atacacccgc tttgcattaa ccacaggcag
    14581 gccgtcccgc tccgactccc gtcgcagcag cgcccacaca cggcgataac catacgtcgg
    14641 caggtcagcc accgccatgt ttatccggga caatacggtg gtatcgtcag aacggggctg
    14701 ccgtctgcga tcctgtcagt cagatggccg gtgaacccga atgctcaact gcgcacgcga
    14761 cacgccaaga cttctgcaaa cgtcgcttat tcgtcgttcc caggcaacga gggcgcatgc
    14821 gcaatccatt tttttgcccg gccgaactcc acggcctctt tgagtatctc ggcttccatc
    14881 gtctttttgc ccagaaggcg ctggagttcg cgaatttgct tattggcggc agcgagttcg
    14941 gaggccggca ccacttcctc gcccgatgca acagccgtca gtgaaccgtc ttaatattgc
    15001 ttgcgccatt tgaatatctg gtttgcattg atgccatgca ggcgcgcgac atgagacacg
    15061 gtcataccgg gctccatagt ctgctgaata atggcaattt tttcctgcgg tgtacgacgc
    15121 cgacgccgct caggtcctga taacacttca accatcttat cgttctgact ggtattaaac
    15181 atagttccaa gactacctct tattttaaga gagtcgaagt gtctggtgat ctatggggcc
    15241 agtctagccc tcttccaagt gtagggagat atagtcaaag tttgcttctc cgccgatgag
    15301 tccgccctgc gtcggatagt ccgatttttt accggcgaag cacggctctc ctaaccctat
    15361 ctaaagcgaa taactttttt caacagggcc gcgtgccaat gcaagagcga taggcgctct
    15421 acgcgccaat ttgaccactt aaaacaggta aattgagggg ttgtaatgtg ttgatgtaac
    15481 aggcttttat tttaatgtct tggcatatgt ataatggtag tctagccctc ctttcaacaa
    15541 ggagtactca tggaaaccta caatcataca tatcggcacc acaacttttc acataaagac
    15601 ttaagtgatc tcaccttcac cgcttgcaca ttcattcgca gcgactttcg acgtgctaac
    15661 ttgcgtgata cgacattcgt caactgcaag ttcattgaac agggtgatat cgaaggctgc
    15721 cactttgatg tcgcagatct tcgtgatgca agtttccaac aatgccaact tgcgatggca
    15781 aacttcagta atgccaattg ctacggtata gagttccgtg cgtgtgattt aaaaggtgcc
    15841 aacttttccc gaacaaactt tgcccatcaa gtgagtaatc gtatgtactt ttgctcagca
    15901 tttatttctg gatgtaatct ttcctatgcc aatatggaga gggtttgttt agaaaaatgt
    15961 gagttgtttg aaaatcgctg gataggaacg aacctagcgg gtgcatcact gaaagagtca
    16021 gacttaagtc gaggtgtttt ttccgaagat gtctgggggc aatttagcct acagggtgcc
    16081 aatttatgcc acgccgaact cgacggttta gatccccgca aagtcgatac atcaggtatc
    16141 aaaattgcag cctggcagca agaactgatt ctcgaagcac tgggtattgt tgtttatcct
    16201 gactaattgc tttgatgtgt gattttaaac gctcaaattt ataaaacgaa taatattgtc
    16261 aaccttgcaa taatagacat agggacaacc atgtataaat ttttaccctc aggcgtcttc
    16321 acaatagagc catatagcag tgggtcactt agtgggctta atttttccat taaagataat
    16381 attaatattg ctcagtataa aacatcatat ggtagcccat cttggcaagt aaacataagg
    16441 ctgccattta taaggcactg ttgcaaaaat gtggaggtga taggggtact acgccggaac
    16501 cccagatttt tccgtccgtt caaattatcc acggttgagc agcttgatcc cgtccgcccg
    16561 cagttgacca acaggggaaa tatgcctgta caaagtttgc cgggttatcc caagttcctg
    16621 gcatagcgta ctgacctttg tttctgattg tcccattgat gccattgcca gtcgcagttt
    16681 gactggcgtc attttataag gtcggccgcc gttccgccca cgggctcttg ctgatgccag
    16741 acctgcagtc gttctttcag caatcagttc gcgctcaaac tccgccagtg cggcaaagat
    16801 accgaagaca agcttgccag cggccgttgt tgtgtcgata gggcactgtt gcaaatagtc
    16861 ggtggtgata aacttatcat ccccttttgc tgatggagct gcacatgaac ccattcaaag
    16921 gccggcattt tcagcgtgac atcattctgt gggccgtacg ctggtactgc aaatacggca
    16981 tcagttaccg tgagctgcag gagatgctgg ctgaacgcgg agtgaatgtc gatcactcca
    17041 cgatttaccg ctgggttcag cgttatgcgc ctgaaatgga aaaacggctg cgctggtact
    17101 ggcgtaaccc ttccgatctt tgcccgtggc acatggatga aacctacgtg aaggtcaatg
    17161 gccgctgggc gtatctgtac cgggccgtcg acagccgggg ccgcactgtc gatttttatc
    17221 tctcctcccg tcgtaacagc aaagctgcat accggtttct gggtaaaatc ctcaacaacg
    17281 tgaagaagtg gcagatccca cgattcatca acacggataa agcgcccgcc tatggtcgcg
    17341 cgcttgctct gctcaaacgc gaaggccggt gcccgtctga cgttgaacac cgacagatta
    17401 agtaccggaa caacgtgatt gaatgcgatc atggcaaact gaaacggata atcaacgcca
    17461 cgctgggatt taaatccatg aagacggctt acgccaccat caaaggtatt gaggtgatgc
    17521 gtgcactacg caaaggccag gcctcagcat tttattatgg tgatcccctg ggcgaaatgc
    17581 gcctggtaag cagagttttt gaaatgtaag gcctttgaat aagacaaaag gctgcctcat
    17641 cgctaacttt gcaacagtgc caacggattt ataccatggc actttaaagc cagcatcact
    17701 gacaatgagc ggtgtggtgt tactcggtag aatgctcgca aggtcggcta gaaattggtc
    17761 atgagctttc tttgaacatt gctctgaaag cgggaacgct ttctcataaa gagtaacaga
    17821 acgaccgtgt agtgcgactg aagctcgcaa taccataagc cgtttttgct cacggatatc
    17881 agaccagtca acaagtacaa tgggcatcgt attgcccgaa cagataaagc tagcatgcca
    17941 acggtataca gcgagtcgct ctttgtggag gtgacgatta cctaacaatc ggtcgattcg
    18001 tttgatgtta tgttttgttc tcgctttggt tggcaggtta cggccaagtt cggtaagagt
    18061 gagagtttta cagtcaagta atgcgtggca agccaacgtt aagctgttga gtcgttttaa
    18121 gtgtaattcg gggcagaatt ggtaaagaga gtcgtgtaaa atatcgagtt cgcacatctt
    18181 gttgtctgat tattgatttt tcgcgaaacc atttgatcat atgacaagat gtgtatccac
    18241 cttaacttaa tgatttttac caaaatcatt aggggattca tcagcgtata gtgttttgca
    18301 gtttagagga gatatcgcga tgcatacgcg gaaggcaata acggaggcgc ttcaaaaact
    18361 cggagtccaa accggtgacc tcttgatggt gcatgcctca cttaaagcga ttggtccggt
    18421 cgaaggagga gcggagacgg tcgttgccgc gttacgctcc gcggttgggc cgactggcac
    18481 tgtgatggga tacgcgtcgt gggaccgatc accctacgag gagactctga atggcgctcg
    18541 gctggatgac gaagcccgcc gtacctggct gccgttcgat cccgcaacag ccgggactta
    18601 ccgtgggttc ggcctgctga atcaatttct ggttcaagcc cccggcgcgc ggcgcagcgc
    18661 gcaccccgat gcatcgatgg tcgcggttgg tccgctggct gaaacgctga cggagcctca
    18721 cgaactcggt cacgccttgg gggaaggatc gcccgtcgag cggttcgttc gccttggcgg
    18781 gaaggccctg ctgttgggtg cgccgctaaa ctccgttacc gcattgcact acgccgaggc
    18841 ggttgccgat atccccaaca aacggtgggt gacgtatgag atgccgatgc ttggaagaga
    18901 cggtgaagtc gcctggaaaa cggcatcgga ttacgattca aacggcattc tcgattgctt
    18961 tgctatcgaa ggaaagccgg atgcggttga aactatagca aatgcttacg tgaagctcgg
    19021 tcgccatcga gaaggtgtcg tgggctttgc tcagtgctac ctgttcgacg cgcaggacat
    19081 cgtgacgttc ggcgtcacct atcttgagaa gcatttcgga accactccga tcgtgcctcc
    19141 gcacgaggcc gtcgagcgct cttgcgagcc ttcaggttag aggccgtcga caatgataat
    19201 ctggatcaac ggacctttcg gcgccggaaa gacgacgctc gctaagcggc tgcgcgatcg
    19261 gcgttccaaa tcgctgatct ttgaccccga ggaaatcggg ttcgtggtga aagaaacggt
    19321 ccccatgcca gcgagcggag actatcagga tctccccttg tggaggggac ttacgatcgc
    19381 ggcggtcagg gagattcgaa ggaattactc gcaggacatc atcatcccaa tgacgctcgt
    19441 gcacccggac tatctgactg agatactcga cggggtaagg cggatcgacg atcagctgct
    19501 gcacatcttt ctgacgctca acgaggacct attgcgtcac cggatcgcga accagaccat
    19561 gcatcctgac ccgaatcgaa atgcggagat tcgagagtgg cgattagcga atgtcgcccg
    19621 atgcttggcc gcaagggaac ggcttccatg cacaacccgt gttctcgata gtggtgcaca
    19681 caccagcgat gaactcgcag cgatggtgct cgacggaatc gatgggcgca cctgatcgcc
    19741 ttcgacgcct gcgcaaagcg tagcgcgagg gtggcgggct cacgaccaaa cgcccagagg
    19801 tcgatcatcg cagggatgtt tggctggcac tgttgcaaag ttagcgatga ggcagccttt
    19861 tgtcttattc aaaggcctta catttcaaaa actctgctta ccaggcgcat ttcgcccagg
    19921 ggatcaccat aataaaatgc tgaggcctgg cctttgcgta gtgcacgcat cacctcaata
    19981 cctttgatgg tggcgtaagc cgtcttcatg gatttaaatc ccagcgtggc gccgattatc
    20041 cgtttcagtt tgccatgatc gcattcaatc acgttgttcc ggtacttaat ctgtcggtgt
    20101 tcaacgtcag acgggcaccg gccttcgcgt ttgagcagag caagcgcgcg accataggcg
    20161 ggcgctttat ccgtgttgat gaatcgcggg atctgccact tcttcacgtt gttgaggatt
    20221 ttacccagaa accggtatgc agctttgctg ttaggggtcg tctcagaaaa cggaaaataa
    20281 agcacgctaa gccggtggca gcggtcgcaa tggcctaaac ttccccgcac cgaccttggc
    20341 gctgctgcgc cataggtaat cgccggtcag gttgatgtgc tcccacccca gcggcgacag
    20401 atattgcaac aatgtgtcgt ccagcgccgt gccgttgcca cgcaaagcac tggtggcacg
    20461 ctccagatat accgtgttcc acaacacgat ggccgccgtc accagattga ggccgctggc
    20521 ccggtagcgc tgctgctcaa aactgcggtc gcggatttca cccaatcggt agaagaagac
    20581 cgccctggcc agcgcgttgc gcgcctcgcc cttattcagc cccgcatgga cgcggcggcg
    20641 cagctccacg ctttgcagcc aatccaaaat gaacagcgtg cgctcgatgc gccccagctc
    20701 gcgcaacgcc acggccaagc cgttctggcg cgggtagctg ccgagtttgc gcagcatcag
    20761 cgaagccgtt accgtgcctt gcttgatgga ggtggccagc cgcagaattt catcccaatg
    20821 ggcgcgtatt tgcttgatgt tcagcctgtc gctgctaatc atcggcttga gcgcgtcata
    20881 ggcggcatcg cccttgggga tgaatagctt ggtttcgccc aagtcacgga tacgcggcgc
    20941 gaagcgaaat cccagcaaat gcatcaagcc aaacacgtga tcggtgaagc ctgccgtgtc
    21001 ggtgtagtgt tcctcgatgc gcaagtccga ctcgtggtac agcaggccat caagcacgta
    21061 agttgaatca cgaatgccca cgttgaccac cttggcactg aagggcgcgt actggtcgga
    21121 gatatgggtg tagaaagtcc gtaagtatct ttactcaaaa gttgcaatca ataatttatt
    21181 gaaaaagatt gaaaggtggt ggtaaataat gttgcaatgt gggagtatct gtaatgaccc
    21241 actgaaatcc tgctcaggtc ataatgagtg atgtctcgtt agaaagtgat tgttggacta
    21301 ttaatggagt aagtatgcct tcccttataa aaataaaaat tgttccgctt atgttctttt
    21361 tggcactgta ttttgcattt atgctgaact ggcgtggagt tctccatttt tacgaaatcc
    21421 tttacaaatt agaagatttt aagtttggtt tcgccatttc attaccaata ttgcttgttg
    21481 cagcgcttaa ctttgtattt gttccatttt cgatacggta tttaataaag cctttttttg
    21541 cacttcttat cgcacttagt gcaatcgtta gttacacaat gatgaagtat agagtcttgt
    21601 ttgatcaaaa catgattcag aatatttttg aaaccaatca aaatgaggcg ttagcatatt
    21661 taagcttacc aattatagta tgggttacta ttgctggttt tatccctgcc attttacttt
    21721 tctttgttga aattgaatat gaggaaaaat ggttcaaagg gattctaact cgtgccctat
    21781 cgatgtttgc atcacttata gtgattgcgg ttattgcagc actatactat caagattatg
    21841 tgtcagtggg gcgcaacaat tcaaacctcc agcgtgagat tgttccagcc aatttcgtta
    21901 atagtaccgt taaatacgtt tacaatcgtt atcttgctga accaatccca tttacaactt
    21961 taggtgatga tgcaaaacgg gatactaatc aaagtaagcc cacgttgatg tttctggtcg
    22021 ttggtgaaac cgctcgtggt aaaaatttct cgatgaatgg ctatgagaaa gacaccaatc
    22081 catttaccag taaatctggt ggcgtgatct cctttaatga tgttcgttcg tgtgggactg
    22141 caaccgctgt atccgtcccc tgcatgttct ccaatatggg gagaaaggag tttgatgata
    22201 atcgcgctcg caatagcgag ggcctgctag atgtgttgca aaaaacgggg atctccattt
    22261 tttggaagga gaacgatgga ggctgcaaag gcgtctgcga ccgagtacct aacatcgaaa
    22321 tcgaaccaaa ggatcaccct aagttctgcg ataaaaacac atgctatgac gaggttgtcc
    22381 ttcaagacct cgatagtgaa attgctcaaa tgaaagggga taagctggtt ggcttccacc
    22441 tgataggtag ccatggccca acctactaca agcgctaccc tgatgctcat cgtcagttca
    22501 cccctgactg tccacgcagt gatattgaaa actgcacaga tgaagagctc accaacacct
    22561 atgacaacac catccgctac accgatttcg tgattggaga gatgattgcc aagttgaaaa
    22621 cctacgaaga taagtacaac accgcgttgc tctacgtctc cgatcatggt gaatcactgg
    22681 gagcattagg gctttaccta cacggtacac cgtaccagtt tgcaccggat gatcagaccc
    22741 gtgttcctat gcaggtgtgg atgtcacctg gatttaccaa agagaaaggc gttgatatgg
    22801 cgtgtttgca gcagaaagcc gctgatactc gttactcaca cgataatatt ttctcatctg
    22861 tattgggtat ctgggacgtc aaaacatcag tttacgaaaa gggtctagat attttcagtc
    22921 aatgtcgtaa tgttcaataa attacattat tgggggctca ggcccccaat catctgttaa
    22981 ataataggtt tattactatt gtccgtgtat ttcatggttg aaatttttaa cgggcaaatc
    23041 agaaagagga agctaaaatg gcaaagcctg gtgcaactgg aattacccga atcattcatg
    23101 ccaccaatta tagtatgaag gggctttctt cagcttggag acatgaagcg gcttttcgtc
    23161 aggaactgct actcattgtg ctgttgatgc ctctggcgtt ttttgtcgga gatggtttaa
    23221 atcaaatact actcctaatg gttataagtt ggctggttgt gattgtagag atcctgaact
    23281 cagctattga agcagtagta gacagaatag ggtcagagca tcatgagctt tctggtcgag
    23341 ctaaagacat gggctctgcg gctgtcttca ttgctctgat gttgaatgcc atagtgtggg
    23401 gtgcattgac agggcgtaat ttattaacct ggtggtagtg ttatcatcaa taatattttt
    23461 aattattcat ggcagccacc ataaatagtg gctgtttaat gaaaaacaat agatgctaat
    23521 tataaggtgg aacttttgtc tgtttagccc gcaccatagg tgcgaaaata accatatttt
    23581 gtaatgaccc actgaaatcc tgctcaggtc ataatgagtg gagatcacag acatgagcat
    23641 cacgatggac gaagaaatca aacgttggac ggccaagcgc aagtccgcct tggtcatgga
    23701 aatcattcag ggcaagacca ccgtcgccga agcggctcga gcctttgatt tgccgccctc
    23761 cgagattgaa gaatgggtag atgacgccaa acggggcatg gaaaacgcct tacgggccaa
    23821 gccactggat attcgcgagc agtacgagaa gcagctcaag gaactccagg aagcttatgg
    23881 cgaggccatg ctggagttac gcgccagaaa aaagctggcc tccctgctgg gcgaggacga
    23941 cagatagtcg ccgatgttca gcagggatta cgcgcagaag gtattgatgt ctccgtcagc
    24001 aagctctgcc gctggtttgg cctgccgcgt cgtagttggt actaccgcag cgtcaaggcc
    24061 gcgcccaagc ttcaggagca tctggtcacc ccgatcaaaa acatgattga ggagaaccca
    24121 tcgttcggtt accggacggt tgcagccctg ttggggttca acaagaacac ggtacagcgc
    24181 atcttccagc tcaagggctg gcaagttcgc aagcgtccgg ttgggtttcg cccccggatc
    24241 caggccctgc cttcggtggc ctcagcgccc aaccaacgct gggcgacgga cctatgccgg
    24301 atctgggctg gtcgcgatgg ctggtgttcc ttggcgctgg tgattgattg ccatacccgt
    24361 gaactactgg gatggcatct ctcgcgcagt ggtaaatcga aaacggccga ggcagcgctg
    24421 gagcaagcct taattgctcg cttcggcagc ttgggacgag ttgcatcgcc gtttttgtta
    24481 cgtagcgaca atgggttggt gtttaccagc cgcaactaca cagcactggt gaagagctat
    24541 gggttgcagc aggagttcat cacgccgtac acgcc
//
