LOCUS       NZ_CP116476             8688 bp    DNA     circular CON 01-MAR-2024
DEFINITION  Pectobacterium sp. A5351 plasmid p_A5351, complete sequence.
ACCESSION   NZ_CP116476
VERSION     NZ_CP116476.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32156144
            Assembly: GCF_028335745.1
KEYWORDS    RefSeq.
SOURCE      Pectobacterium sp. A5351
  ORGANISM  Pectobacterium sp. A5351
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Pectobacteriaceae; Pectobacterium.
REFERENCE   1  (bases 1 to 8688)
  AUTHORS   Klair,D., Arizala,D., Dobhal,S., Arif,M. and Alvarez,A.
  TITLE     Pectobacterium aglaonemum_A5351
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8688)
  AUTHORS   Klair,D., Arizala,D., Dobhal,S., Arif,M. and Alvarez,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JAN-2023) PEPS, University of Hawaii at Manoa, 3190
            Maile Way, Honolulu, HI 96822, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 226.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/01/2024 01:43:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,053
            CDSs (total)                      :: 3,944
            Genes (coding)                    :: 3,785
            CDSs (with protein)               :: 3,785
            Genes (RNA)                       :: 109
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 75 of 159
            Pseudo Genes (incomplete)         :: 123 of 159
            Pseudo Genes (internal stop)      :: 28 of 159
            Pseudo Genes (multiple problems)  :: 56 of 159
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116476.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8688
                     /organism="Pectobacterium sp. A5351"
                     /mol_type="genomic DNA"
                     /strain="A5351"
                     /isolation_source="Aglaonema"
                     /db_xref="taxon:2914983"
                     /plasmid="p_A5351"
                     /geo_loc_name="USA: Oahu, Hawaii"
                     /lat_lon="21.4389 N 158.0001 W"
                     /collection_date="2007"
                     /collected_by="Anne A Alvarez"
     gene            1..840
                     /locus_tag="O1Q74_RS00005"
                     /old_locus_tag="O1Q74_00005"
     CDS             1..840
                     /locus_tag="O1Q74_RS00005"
                     /old_locus_tag="O1Q74_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_000190708.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            827..1678
                     /gene="repC"
                     /locus_tag="O1Q74_RS00010"
                     /old_locus_tag="O1Q74_00010"
     CDS             827..1678
                     /gene="repC"
                     /locus_tag="O1Q74_RS00010"
                     /old_locus_tag="O1Q74_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            1986..2801
                     /gene="sul2"
                     /locus_tag="O1Q74_RS00015"
                     /old_locus_tag="O1Q74_00015"
     ARG             1987..2798
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             3247..3665
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="52.06"
     ARG             2863..3662
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            3665..4501
                     /locus_tag="O1Q74_RS00025"
                     /old_locus_tag="O1Q74_00025"
     CDS             3665..4501
                     /locus_tag="O1Q74_RS00025"
                     /old_locus_tag="O1Q74_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            complement(<4608..5012)
                     /locus_tag="O1Q74_RS00030"
                     /old_locus_tag="O1Q74_00030"
                     /pseudo=""
     CDS             complement(<4608..5012)
                     /locus_tag="O1Q74_RS00030"
                     /old_locus_tag="O1Q74_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(5566..5850)
                     /locus_tag="O1Q74_RS00035"
                     /old_locus_tag="O1Q74_00035"
     CDS             complement(5566..5850)
                     /locus_tag="O1Q74_RS00035"
                     /old_locus_tag="O1Q74_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WP_000238497.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            6049..>6690
                     /locus_tag="O1Q74_RS00040"
                     /old_locus_tag="O1Q74_00040"
                     /pseudo=""
     CDS             6049..>6690
                     /locus_tag="O1Q74_RS00040"
                     /old_locus_tag="O1Q74_00040"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            6797..7210
                     /locus_tag="O1Q74_RS00045"
                     /old_locus_tag="O1Q74_00045"
     CDS             6797..7210
                     /locus_tag="O1Q74_RS00045"
                     /old_locus_tag="O1Q74_00045"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010890157.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            7309..8178
                     /locus_tag="O1Q74_RS00050"
                     /old_locus_tag="O1Q74_00050"
     CDS             7309..8178
                     /locus_tag="O1Q74_RS00050"
                     /old_locus_tag="O1Q74_00050"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_229382459.1"
                     /translation="MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDL
                     SEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASA
                     DSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQ
                     EKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRS
                     AEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMD
                     RGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            8239..8451
                     /locus_tag="O1Q74_RS00055"
                     /old_locus_tag="O1Q74_00055"
     CDS             8239..8451
                     /locus_tag="O1Q74_RS00055"
                     /old_locus_tag="O1Q74_00055"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455501.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            8453..8659
                     /gene="urfF"
                     /locus_tag="O1Q74_RS00060"
                     /old_locus_tag="O1Q74_00060"
     CDS             8453..8659
                     /gene="urfF"
                     /locus_tag="O1Q74_RS00060"
                     /old_locus_tag="O1Q74_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_007857788.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     ARG             3666..4498
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          5805..5960
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          19..840
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          6050..8175
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctaccc ataagcctat caatattctg gaggcgttcg cagcagcgcc gccaccgctg
       61 gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc gcccggtggt
      121 gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg gccggatctg
      181 ctggaggtgg gcgaactgcc caccggcccg gtgatctacc tgcccgccga agacccgccc
      241 accgccattc atcaccgcct gcacgccctt ggggcgcacc tcagcgccga ggaacggcaa
      301 gccgtggctg acggcctgct gatccagccg ctgatcggca gcctgcccaa catcatggcc
      361 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgctggac
      421 acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc ccaggtcatc
      481 ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggcagctcg
      601 gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag cgccgaggcc
      661 gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg tgtgagcaag
      721 gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg cggggtgctc
      781 aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg tgaagcctaa
      841 gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg cccccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg atgatctgcg
     1021 catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg gcccggaacc
     1081 caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg aggccgtcac
     1141 cgctgatgcc atggtggtca aaggtagcta tcgggcgctg gcaaaggaaa tcggggcaga
     1201 ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc tttggaaggt
     1261 atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt cggagtacgc
     1321 cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg cgcaggccgt
     1381 catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc tggacagcga
     1441 aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca aaaccggcaa
     1501 ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     1561 gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc tgggctggac
     1621 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     1681 ccccccactc tattgtaaac aagacatttt tatcttttat attcaatggc ttattttcct
     1741 gctaattggt aataccatga aaaataccat gctcagaaaa ggcttaacaa tattttgaaa
     1801 aattgcctac tgagcgctgc cgcacagctc cataggccgc tttcctggct ttgcttccag
     1861 atgtatgctc ttctgctcct gcagctaatg gatcaccgca aacaggttac tcgcctgggg
     1921 attccctttc gacccgagca tccgtatgat actcatgctc gattattatt attatagaag
     1981 cccccatgaa taaatcgctc atcattttcg gcatcgtcaa cataacctcg gacagtttct
     2041 ccgatggagg ccggtatctg gcgccagacg cagccattgc gcaggcgcgt aagctgatgg
     2101 ccgagggggc agatgtgatc gacctcggtc cggcatccag caatcccgac gccgcgcctg
     2161 tttcgtccga cacagaaatc gcgcgtatcg cgccggtgct ggacgcgctc aaggcagatg
     2221 gcattcccgt ctcgctcgac agttatcaac ccgcgacgca agcctatgcc ttgtcgcgtg
     2281 gtgtggccta tctcaatgat attcgcggtt ttccagacgc tgcgttctat ccgcaattgg
     2341 cgaaatcatc tgccaaactc gtcgttatgc attcggtgca agacgggcag gcagatcggc
     2401 gcgaggcacc cgctggcgac atcatggatc acattgcggc gttctttgac gcgcgcatcg
     2461 cggcgctgac gggtgccggt atcaaacgca accgccttgt ccttgatccc ggcatggggt
     2521 tttttctggg ggctgctccc gaaacctcgc tctcggtgct ggcgcggttc gatgaattgc
     2581 ggctgcgctt cgatttgccg gtgcttctgt ctgtttcgcg caaatccttt ctgcgcgcgc
     2641 tcacaggccg tggtccgggg gatgtcgggg ccgcgacact cgctgcagag cttgccgccg
     2701 ccgcaggtgg agctgacttc atccgcacac acgagccgcg ccccttgcgc gacgggctgg
     2761 cggtattggc ggcgctgaaa gaaaccgcaa gaattcgtta actgcacatt cgggatattt
     2821 ctctatattc gcgcttcatc agaaaactga aggaacctcc attgaatcga actaatattt
     2881 tttttggtga atcgcattct gactggttgc ctgtcagagg cggagaatct ggtgattttg
     2941 tttttcgacg tggtgacggg catgccttcg cgaaaatcgc acctgcttcc cgccgcggtg
     3001 agctcgctgg agagcgtgac cgcctcattt ggctcaaagg tcgaggtgtg gcttgccccg
     3061 aggtcatcaa ctggcaggag gaacaggagg gtgcatgctt ggtgataacg gcaattccgg
     3121 gagtaccggc ggctgatctg tctggagcgg atttgctcaa agcgtggccg tcaatggggc
     3181 agcaacttgg cgctgttcac agcctatcgg ttgatcaatg tccgtttgag cgcaggctgt
     3241 cgcgaatgtt cggacgcgcc gttgatgtgg tgtcccgcaa tgccgtcaat cccgacttct
     3301 taccggacga ggacaagagt acgccgcagc tcgatctttt ggctcgtgtc gaacgagagc
     3361 taccggtgcg gctcgaccaa gagcgcaccg atatggttgt ttgccatggt gatccctgca
     3421 tgccgaactt catggtggac cctaaaactc ttcaatgcac gggtctgatc gaccttgggc
     3481 ggctcggaac agcagatcgc tatgccgatt tggcactcat gattgctaac gccgaagaga
     3541 actgggcagc gccagatgaa gcagagcgcg ccttcgctgt cctattcaat gtattgggga
     3601 tcgaagcccc cgaccgcgaa cgccttgcct tctatctgcg attggaccct ctgacttggg
     3661 gttgatgttc atgccgcctg tttttcctgc tcattggcac gtttcgcaac ctgttctcat
     3721 tgcggacacc ttttccagcc tcgtttggaa agtttcattg ccagacggga ctcctgcaat
     3781 cgtcaaggga ttgaaaccta tagaagacat tgctgatgaa ctgcgcgggg ccgactatct
     3841 ggtatggcgc aatgggaggg gagcagtccg gttgctcggt cgtgagaaca atctgatgtt
     3901 gctcgaatat gccggggagc gaatgctctc tcacatcgtt gccgagcacg gcgactacca
     3961 ggcgaccgaa attgcagcgg aactaatggc gaagctgtat gccgcatctg aggaacccct
     4021 gccttctgcc cttctcccga tccgggatcg ctttgcagct ttgtttcagc gggcgcgcga
     4081 tgatcaaaac gcaggttgtc aaactgacta cgtccacgcg gcgattatag ccgatcaaat
     4141 gatgagcaat gcctcggaac tgcgtgggct acatggcgat ctgcatcatg aaaacatcat
     4201 gttctccagt cgcggctggc tggtgataga tcccgtcggt ctggtcggtg aagtgggctt
     4261 tggcgccgcc aatatgttct acgatccggc tgacagagac gacctttgtc tcgatcctag
     4321 acgcattgca cagatggcgg acgcattctc tcgtgcgctg gacgtcgatc cgcgtcgcct
     4381 gctcgaccag gcgtacgctt atgggtgcct ttccgcagct tggaacgcgg atggagaaga
     4441 ggagcaacgc gatctagcta tcgcggccgc gatcaagcag gtgcgacaga cgtcatacta
     4501 gatatcaagc gacttctcct atcccctggg aacacatcaa tctcaccgga gaatatcgct
     4561 ggccaaagcc ttagcgtagg attccgcccc ttcccgcaaa cgaccccaaa caggaaacgc
     4621 agctgaaacg ggaagctcaa cacccactga cgcatgggtt gttcaggcag tacttcatca
     4681 accagcaagg cggcactttc ggccatccgc cgcgccccac agctcgggca gaaaccgcga
     4741 cgcttacagc tgaaagcgac caggtgctcg gcgtggcaag actcgcagcg aacccgtaga
     4801 aagccatgct ccagccgccc gcattggaga aattcttcaa attcccgttg cacatagccc
     4861 ggcaattcct ttccctgctc tgccataagc gcagcgaatg ccgggtaata ctcgtcaacg
     4921 atctgataga gaagggtttg ctcgggtcgg tggctctggt aacgaccagt atcccgatcc
     4981 cggctggccg tcctggccgc cacatgaggc atgttccgcg tccttgcaat actgtgttta
     5041 catacagtct atcgcttagc ggaaagttct tttaccctca gccgaaatgc ctgccgttgc
     5101 tagacattgc cagccagtgc ccgtcactcc cgtactaact gtcacgaacc cctgcaataa
     5161 ctgtcacgcc cccctgcaat aactgtcacg aacccctgca ataactgtca cgcccccaaa
     5221 cctgcaaacc cagcaggggc gggggctggc ggggtgttgg aaaaatccat ccatgattat
     5281 ctaagaataa tccactaggc gcggttatca gcgcccttgt ggggcgctgc tgcccttgcc
     5341 caatatgccc ggccagaggc cggatagctg gtctattcgc tgcgctaggc tacacaccgc
     5401 cccaccgctg cgcggcaggg ggaaaggcgg gcaaagcccg ctaaacccca caccaaaccc
     5461 cgcagaaata cgctggagcg cttttagccg ctttagcggc ctttccccct acccgaaggg
     5521 tgggggcgcg tgtgcagccc cgcagggcct gtctcggtcg atcattcagc ccggctcatc
     5581 cttctggcgt ggcggcagac cgaacaaggc gcggtcgtgg tcgcgttcaa ggtacgcatc
     5641 cattgccgcc atgagccgat cctccggcca ctcgctgctg ttcaccttgg ccaaaatcat
     5701 ggcccccacc agcaccttgc gccttgtttc gttcttgcgc tcttgctgct gttcccttgc
     5761 ccgcacccgc tgaatttcgg cattgattcg cgctcgttgt tcttcgagct tggccagccg
     5821 atccgccgcc ttgttgctcc ccttaaccat cttgacaccc cattgttaat gtgctgtctc
     5881 gtaggctatc atggaggcac agcggcggca atcccgaccc tactttgtag gggagggcgc
     5941 acttaccggt ttctcttcga gaaactggcc taacggccac ccttcgggcg gtgcgctctc
     6001 cgagggccat tgcatggagc cgaaaagcaa aagcaacagc gaggcagcat ggcgatttat
     6061 caccttacgg cgaaaaccgg cagcaggtcg ggcggccaat cggccagggc caaggccgac
     6121 tacatccagc gcgaaggcaa gtatgcccgc gacatggatg aagtcttgca cgccgaatcc
     6181 gggcacatgc cggagttcgt cgagcggccc gccgactact gggatgctgc cgacctgtat
     6241 gaacgcgcca atgggcggct gttcaaggag gtcgaatttg ccctgccggt cgagctgacc
     6301 ctcgaccagc agaaggcgct ggcgtccgag ttcgcccagc acctgaccgg tgccgagcgc
     6361 ctgccgtata cgctggccat ccatgccggt ggcggcgaga acccgcactg ccacctgatg
     6421 atctccgagc ggatcaatga cggcatcgag cggcccgccg ctcagtggtt caagcggtac
     6481 aacggcaaga ccccggagaa gggcggggca cagaagaccg aagcgctcaa gcccaaggca
     6541 tggcttgagc agacccgcga ggcatgggcc gaccatgcca accgggcatt agagcgggct
     6601 ggccacgacg cccgcattga ccacagaaca cttgaggcgc agggcatcga gcgcctgccc
     6661 ggtgttcacc tggggccgaa cgtggtggag atggaaggcc ggggcatccg caccgaccgg
     6721 gcagacgtgg ccctgaacat cgacaccgcc aacgcccaga tcatcgactt acaggaatac
     6781 cgggaggcaa tagaccatga acgcaatcga cagagtgaag aaatccagag gcatcaacga
     6841 gttagcggag cagatcgaac cgctggccca gagcatggcg acactggccg acgaagcccg
     6901 gcaggtcatg agccagaccc agcaggccag cgaggcgcag gcggcggagt ggctgaaagc
     6961 ccagcgccag acaggggcgg catgggtgga gctggccaaa gagttgcggg aggtagccgc
     7021 cgaggtgagc agcgccgcgc agagcgcccg gagcgcgtcg cgggggtggc actggaagct
     7081 atggctaacc gtgatgctgg cttccatgat gcctacggtg gtgctgctga tcgcatcgtt
     7141 gctcttgctc gacctgacgc cactgacaac cgaggacggc tcgatctggc tgcgcttggt
     7201 ggcccgatga agaacgacag gactttgcag gccataggcc gacagctcaa ggccatgggc
     7261 tgtgagcgct tcgatatcgg cgtcagggac gccaccaccg gccagatgat gaaccgggaa
     7321 tggtcagccg ccgaagtgct ccagaacacg ccatggctca agcggatgaa tgcccagggc
     7381 aatgacgtgt atatcaggcc cgccgagcag gagcggcatg gtctggtgct ggtggacgac
     7441 ctcagcgagt ttgacctgga tgacatgaaa gccgagggcc gggagcctgc cctggtagtg
     7501 gaaaccagcc cgaagaacta tcaggcatgg gtcaaggtgg ccgacgccgc aggcggtgaa
     7561 cttcgggggc agattgcccg gacgctggcc agcgagtacg acgccgaccc ggccagcgcc
     7621 gacagccgcc actatggccg cttggcgggc ttcaccaacc gcaaggacaa gcacaccacc
     7681 cgcgccggtt atcagccgtg ggtgctgctg cgtgaatcca agggcaagac cgccaccgct
     7741 ggcccggcgc tggtgcagca ggctggccag cagatcgagc aggcccagcg gcagcaggag
     7801 aaggcccgca ggctggccag cctcgaactg cccgagcggc agcttagccg ccaccggcgc
     7861 acggcgctgg acgagtaccg cagcgagatg gccgggctgg tcaagcgctt cggtgatgac
     7921 ctcagcaagt gcgactttat cgccgcgcag aagctggcca gccggggccg cagtgccgag
     7981 gaaatcggca aggccatggc cgaggccagc ccagcgctgg cagagcgcaa gcccggccac
     8041 gaagcggatt acatcgagcg caccgtcagc aaggtcatgg gtctgcccag cgtccagctt
     8101 gcgcgggccg agctggcacg ggcaccggca ccccgccagc gaggcatgga caggggcggg
     8161 ccagatttca gcatgtagtg cttgcgttgg tactcacgcc tgttatacta tgagtactca
     8221 cgcacagaag ggggttttat ggaatacgaa aaaagcgctt cagggtcggt ctacctgatc
     8281 aaaagtgaca agggctattg gttgcccggt ggctttggtt atacgtcaaa caaggccgag
     8341 gctggccgct tttcagtcgc tgatatggcc agccttaacc ttgacggctg caccttgtcc
     8401 ttgttccgcg aagacaagcc tttcggcccc ggcaagtttc tcggtgactg atatgaaaga
     8461 ccaaaaggac aagcagaccg gcgacctgct ggccagccct gacgctgtac gccaagcgcg
     8521 atatgccgag cgcatgaagg ccaaagggat gcgtcagcgc aagttctggc tgaccgacga
     8581 cgaatacgag gcgctgcgcg agtgcctgga agaactcaga gcggcgcagg gcgggggtag
     8641 tgaccccgcc agcgcctaac caccaactgc ctgcaaagga ggcaatca
//
