LOCUS       NZ_CP116336             8548 bp    DNA     circular CON 01-MAR-2024
DEFINITION  Bacillus thuringiensis strain BLB1 plasmid pBLB1_8548, complete
            sequence.
ACCESSION   NZ_CP116336
VERSION     NZ_CP116336.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32746260
            Assembly: GCF_028356755.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 8548)
  AUTHORS   Fayad,N., Barssoum,R., Marsaud,N., Nasserdinne,R., Abdelmalek,N.,
            Teste,M.A., Pailler,V., Gautier,V., Belmonte,E., Aceves,C.,
            Cescut,J., Fillaudeau,L. and Kallassy Awad,M.
  TITLE     Complete genomes of two Bacillus thuringiensis serovar kurstaki
            strains isolated from Lebanon and Tunisia, highly toxic against
            lepidopteran larvae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8548)
  AUTHORS   Fayad,N., Barssoum,R., Marsaud,N., Nasserdinne,R., Abdelmalek,N.,
            Teste,M.A., Pailler,V., Gautier,V., Belmonte,E., Aceves,C.,
            Cescut,J., Fillaudeau,L. and Kallassy Awad,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2023) Faculty of Science, Saint Joseph University
            of Beirut, Mar Roukoz, Mansourieh, Beirut 1514, Lebanon
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. v2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: ASM71753v1
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/01/2024 02:57:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,608
            CDSs (total)                      :: 6,457
            Genes (coding)                    :: 6,143
            CDSs (with protein)               :: 6,143
            Genes (RNA)                       :: 151
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 314
            CDSs (without protein)            :: 314
            Pseudo Genes (ambiguous residues) :: 0 of 314
            Pseudo Genes (frameshifted)       :: 114 of 314
            Pseudo Genes (incomplete)         :: 220 of 314
            Pseudo Genes (internal stop)      :: 67 of 314
            Pseudo Genes (multiple problems)  :: 69 of 314
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116336.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8548
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="BLB1"
                     /isolation_source="soil sample"
                     /db_xref="taxon:1428"
                     /plasmid="pBLB1_8548"
                     /geo_loc_name="Tunisia"
                     /collection_date="2008-01-01"
     gene            complement(join(8523..8548,1..826))
                     /locus_tag="PJW01_RS33010"
                     /old_locus_tag="PJW01_33010"
     CDS             complement(join(8523..8548,1..826))
                     /locus_tag="PJW01_RS33010"
                     /old_locus_tag="PJW01_33010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_277662111.1"
                     /translation="MNVQLGNEQITKLLNDWYQEIRAQHIIKAKQLKQKIEKEINNIEE
                     DQNLLLYYSLIDLRYKILTNDYADREQSLEKIEQLKEHTNNFLDYYYHFFKGMHAMKTG
                     NYSETQKYYDIAEKLLEEIPDEVEKAEFNYAVATFYYHTHQALLATQYANKAKSFFSGK
                     LGYEIKTGACENTLGMACITLREFSVAEEYLLSAINKFEKHSENKLALIVRYNLGLLYA
                     DQNLSELAIRYLLESFENQEDDYKTMFLLAQEYYKLKQTNTVNTYIEKGLNVCKTHILK
                     KG"
                     /name="tetratricopeptide repeat protein"
     gene            complement(1298..1525)
                     /locus_tag="PJW01_RS33015"
                     /old_locus_tag="PJW01_33015"
     CDS             complement(1298..1525)
                     /locus_tag="PJW01_RS33015"
                     /old_locus_tag="PJW01_33015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869869.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000869869.1"
                     /translation="MKVVSNIRMIMAKKNIDNISDLVRITGVSRNSINKLWHNENVSSL
                     KLDTLITICEKLDVELLDLIEYIRDDSETK"
                     /name="helix-turn-helix transcriptional regulator"
     gene            1799..2062
                     /locus_tag="PJW01_RS33020"
                     /old_locus_tag="PJW01_33020"
     CDS             1799..2062
                     /locus_tag="PJW01_RS33020"
                     /old_locus_tag="PJW01_33020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000874967.1"
                     /translation="MAFLTNYKANGKRYFYVEKYVGKKPYTCKQSERIYSIGNERITLE
                     RLTLWILDNSFIPNELIKIGISINDIENWREKVENTIKRYSL"
                     /name="hypothetical protein"
     gene            complement(2931..3128)
                     /locus_tag="PJW01_RS33025"
                     /old_locus_tag="PJW01_33025"
     CDS             complement(2931..3128)
                     /locus_tag="PJW01_RS33025"
                     /old_locus_tag="PJW01_33025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000861196.1"
                     /translation="MKVAGDLYYYCLGCKKFHEYEKIDHKGVNRKLCFYCFKIQSKKTK
                     IIGDAEGRMQICETCHKELF"
                     /name="hypothetical protein"
     gene            complement(3132..4178)
                     /locus_tag="PJW01_RS33030"
                     /old_locus_tag="PJW01_33030"
     CDS             complement(3132..4178)
                     /locus_tag="PJW01_RS33030"
                     /old_locus_tag="PJW01_33030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085408.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001085408.1"
                     /translation="MAKKNQDEITGKLQPKKKQNIKIYEFIKKKMSESGKELFKSCSIF
                     NEFLATKDKKKKKRVKGNDCKNRFCPICAWRKAGKDAVKIATMMEAIKIEEKKEFLFLT
                     LTTPNIKADMVKSEIDRFNKAFKKLFDRRNIQRSIKGYIRKLEMTYDKERFITEEMYNN
                     KKRKAYYDSRGLKVDDHNPNYDTYNPHFHVLLCVDKNYFKRKELYIKQEEWLEMWREVT
                     DMPEITQVHIQKVELIREGNAVAEVAKYSAKDYEMSVSQEVFDVFYLALKGRQLIVYGG
                     LLKDYAKKYEDGELDKYKSTDKNEYYYRLIAMWNKDLMKFEQEYQELSESEKQEYNGHL
                     INEVDVEA"
                     /name="protein rep"
     gene            complement(4648..5853)
                     /gene="mobV"
                     /locus_tag="PJW01_RS33035"
                     /old_locus_tag="PJW01_33035"
     CDS             complement(4648..5853)
                     /gene="mobV"
                     /locus_tag="PJW01_RS33035"
                     /old_locus_tag="PJW01_33035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033080.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000033305.1"
                     /translation="MSKFVICHMQKFKMRDVKGLQIHNQREKESHSNSDIIQARTEQNY
                     DLIHDKEKVDYKKLIQNRIDNGVVSNRAIRKDAVVCCSFMISASPDYMNSLSQVEQRRF
                     FEESLRFLKGRYGEENFVYASVHVDEKTPHMHVGMVPVNEKQKLSAYSFFKNKSEFHDL
                     QDKIYEHVKEKGFDIERGVSSDRKHLSTQRFKAVTLQQEIEELEQEKKGIDSRLHDLKM
                     SLDQAKSVDEIPVKEKGGFIRSKTVEITSEDFESIKVLARSSEALREDVRRLKNESGVI
                     TREKDGLYREKCILEGQITELKRENRGLKEENDFFKKTLERVKELYKEKLPELAGVIGY
                     VKGSILDRMNCKFLKRYFTSDDEVRGAQKFLNHKQEHEEQQKRLKQVRSSQQKNRDQGL
                     ER"
                     /name="mobV"
     gene            complement(7226..7423)
                     /locus_tag="PJW01_RS33040"
                     /old_locus_tag="PJW01_33040"
     CDS             complement(7226..7423)
                     /locus_tag="PJW01_RS33040"
                     /old_locus_tag="PJW01_33040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000361625.1"
                     /translation="MDKSSILKQMYFILFPVTLFLSLNIYQWIVKSDYSFTGYIIQNLI
                     IVALALVAAIIAYLITKKNI"
                     /name="hypothetical protein"
     gene            complement(7416..7997)
                     /locus_tag="PJW01_RS33045"
                     /old_locus_tag="PJW01_33045"
     CDS             complement(7416..7997)
                     /locus_tag="PJW01_RS33045"
                     /old_locus_tag="PJW01_33045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000723966.1"
                     /translation="MKKISIERSNWRKTYKKMIPVLLVGGILAGNVAFVTNNVFAATTN
                     NNNIIQTKQSLVSGQDEKSINELAEAFQFMHEEAAIYNNQGEIVDFDFNKIEAKYGNDV
                     GLDALKQQIKKDKSDIAGLSFTSCMKDALYDFFGVNAVQAAFNGGLAYYLEKKLWKEAA
                     KIGVKFFIGSSVAGLAATLAYYGGKCAIYG"
                     /name="hypothetical protein"
     gene            complement(8117..8263)
                     /locus_tag="PJW01_RS33050"
                     /old_locus_tag="PJW01_33050"
     CDS             complement(8117..8263)
                     /locus_tag="PJW01_RS33050"
                     /old_locus_tag="PJW01_33050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000976019.1"
                     /translation="MMKKFSLILIGVACTTGIFFSQFNNSIQTHDAKEKNDIIQQYAHG
                     KDI"
                     /name="hypothetical protein"
     Typing          complement(3151..4203)
                     /name="rep_cluster_617"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.934"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(4652..5853)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tacaaacatt tagccctttt tcaatatatg tgtttactgt atttgtttgt tttaacttgt
       61 aatattcttg tgctaataaa aacattgttt tgtaatcgtc ttcttgattt tcaaaggatt
      121 caagaagata acggattgca agttcagaaa gattttgatc tgcatataaa agacctaagt
      181 tataacgtac gattaaagct aatttgtttt cactgtgttt ttcaaattta ttaattgcgg
      241 aaagcaagta ttcttctgca acagaaaatt cccttaatgt aatacaagcc ataccaagtg
      301 tgttttcaca tgctccggtt ttaatctcgt atcctagttt cccactaaaa aaacttttgg
      361 ctttgttagc atattgagtt gctaataagg cttgatgagt atgataatag aaagttgcaa
      421 cagcataatt aaattcagct ttttctactt catcaggaat ttcttctagt aatttttctg
      481 ctatatcata atatttttga gtttcactat aatttcctgt tttcattgca tgcatgcctt
      541 taaagaaatg ataatagtaa tctaaaaaat tatttgtgtg ttcttttaat tgttcgattt
      601 tttctaaaga ttgttctcta tctgcatagt cgtttgtaag aatcttgtat ctcaaatcaa
      661 taagtgaata atataaaagt aaattttggt cttcttctat gttgttaatt tctttttcaa
      721 tcttttgttt taattgtttt gctttaataa tgtgttgcgc tcgaatttct tgataccaat
      781 catttaataa ttttgtaatt tgttcgtttc ctagttgaac attcatagaa tcttctcctt
      841 ttccaaaatt atctatattt tattttctat catgttatca tgctattgta taagactaac
      901 ttttcaacag ttgatatata aagtttttaa tattctgtat ttttaaatag tgtagcacct
      961 tcactattta aaaatactta gtaacaaata aaaaaagagc ccccaaacag gaactctttt
     1021 tcaatactca tcaattccat cactttactg cactactatc aatgaaatca cgaagtatgc
     1081 cagtaccgct catcttccaa ggtgttgacg cacctagcaa gactataatt tcatttacca
     1141 cattcaaacc atattcatac aataacaaat agaaatcaaa actttaaata cacatatagc
     1201 cctacaaaaa aaacatatgt gaatataact cattgtcgat tagcaaaaat atgctttcat
     1261 catacacaag ctatctaaag ctaaaatagc ccctaaatta ttttgtctca ctatcatctc
     1321 gaatatattc aattaaatcc aataactcaa catccaactt ctcacagatc gttatcaatg
     1381 tatctaattt taaagaagac acattctcat tgtgccacaa cttattaata gaattacgac
     1441 ttactcccgt tattctcacc aaatccgaaa tattatctat attctttttc gccataatca
     1501 tacgaatatt acttactacc ttcatttttc atactccttt tcattcaatg tacataccat
     1561 tttacttaca aggatacttt tatacaaata aaacataaat taaataatat taaggttatt
     1621 ttttatatat tcacatttaa tattaacctt aaaaataaaa tatattgttt tattatttaa
     1681 ttttatttgt tttaattgaa attttaaccc cttagagtta aaattaaata tataacttaa
     1741 aatttaatct tttaaaatgt atttttaact ctaaaaaaac taagaggaga ttatcatttt
     1801 ggcattccta acaaattaca aagcaaacgg aaaaagatac ttttatgtag aaaaatatgt
     1861 tggtaaaaaa ccatatactt gcaaacaaag tgaacgtatt tattcaattg gaaacgaaag
     1921 aatcacatta gaaaggctca ctttatggat tttagataac agtttcattc caaacgaatt
     1981 aataaagatc ggaatatcaa ttaacgatat agagaactgg agagaaaaag tagaaaacac
     2041 cataaagaga tactctttat aaataaaact aaagaaaaaa gactgatatt ttttatcagt
     2101 cttttttctt taactattta tattttttca tcataaaagg gttaggtatt ttaataatat
     2161 atttatatat ttaataaaag ttacgcgtga ctttttgatt ttttcatttg cgcacaaaag
     2221 caaaaagatt ttaagagtaa aaaggattaa aaatatacag aaatcgcgaa ataaaaaatt
     2281 cacagaaaca acaatttcaa acataatcta aacatctttt tatcacacaa ctcgtttgaa
     2341 acggtgcaac tttttaacga tatgaaatat ctatgaatta aatatgcgag caaagttttt
     2401 tcgttttcag ttcgtcatga aggtattata taaactcctt aatttcgaaa tacagaataa
     2461 caaagaaaag agaaaacaaa aattccgaaa atcgaaaaat cgagatttcc aatttaacaa
     2521 attccaaaat caaatcccac ataaaaaatc caaaatcagc cccgaaaatt tagaatttct
     2581 caaaactata ttttcaaaaa aatcatcaaa aaacgtgcgg tattttatcg taatatatat
     2641 tactaaaaaa attcatgcgc acactttaag ttttcacctc gtacgttagt aaaacaaaat
     2701 tagtagaaca aaatatatct cgaaataaac tttaaaaata aatttacaaa ttcacgaaat
     2761 ttaaatttaa gaaaaactcc tttttgttaa atcataaaaa aacttttgaa tgctacttgc
     2821 tgcactcaaa aatagactca ataaaataag ggaactgact tgatataaca gtcagttccc
     2881 ttattaaaaa tttctaacat ttgactttgc ttttttcaca aatcaaccaa ctaaaacaat
     2941 tctttatgac aagtctcaca aatttgcatt ctaccttcag catcaccaat aattttcgtt
     3001 tttttagatt gaatcttgaa acaataaaaa cataattttc tatttacacc tttatgatca
     3061 attttttcat attcatgaaa ctttttacaa ccaagacaat aataatataa atctcccgct
     3121 actttcatac actacgcctc cacatctact tcattaatta aatgaccgtt atactcttgc
     3181 ttctcactct cagataactc ttgatactct tgttcaaatt tcattaaatc cttattccac
     3241 attgcgatta atctataata atattcattc ttgtctgtac tcttatactt gtccaattcg
     3301 ccatcttcat attttttcgc ataatctttt aataatccac cgtaaacaat taattgacga
     3361 ccttttaatg ctaaatagaa cacgtcaaac acttcttgac taaccgacat ttcatagtct
     3421 ttcgctgaat acttcgcaac ctccgcaact gcatttcctt ctcgtattaa ctctactttt
     3481 tgaatatgta cttgcgtaat ctctggcata tctgttactt ctcgccacat ttctaaccat
     3541 tcttcttgct taatataaag ctctttacgt ttaaaataat ttttatcaac acaaagcaat
     3601 acatgaaaat gaggattata tgtatcataa ttcgggttat gatcgtctac tttcaatcca
     3661 cgagaatcat aatatgcttt tctctttttg ttgttataca tttcttcagt gatgaatcgt
     3721 tctttatcat acgtcatttc tagctttcta atataaccct taatagaacg ttgaatatta
     3781 cgtctatcaa ataacttttt aaaagcttta ttaaatctat caatctcact tttcaccata
     3841 tcagctttaa tatttggagt cgtcaacgtt aaaaaaagaa actctttctt ttcctcaatc
     3901 ttaattgctt ccatcatcgt tgcgattttc accgcgtcct tccctgcttt cctccaagca
     3961 caaatcggac aaaatcgatt tttacaatca ttccccttta ctcgtttctt cttcttttta
     4021 tcctttgtag ccaaaaattc attaaagata ctgcacgatt taaacaattc tttcccgctc
     4081 tcactcattt tcttctttat aaattcgtaa atcttgatat tctgtttctt tttaggttgt
     4141 aatttaccag tgatttcatc ttgatttttc tttgccatat ttgatatatt agtcctatct
     4201 gaaaaacatc agaatttcta atttctgaat ttcatattcg atataaaaaa gggtgtacga
     4261 atcctttttt tggacttgct cctctcgtgg tatttatgat ttttaattcg acactaaaaa
     4321 tgataaatct atccgcctga aaaagcaagt cttttttttt acaaaccctt gatacataag
     4381 ggctaaaaga atacataggc atcaatttcg ttgccgaaat agttacctat gtattaaaga
     4441 aagaaaaaac tcgatttttc gaacacgaaa aatcataaaa aaatgcccct aaatccactc
     4501 cgtggacagg ggcatttttt ttcgttcaaa accatttttt ttaacggata aaacatctga
     4561 gaaacatact catcttaagc aagttataaa aaacaaattc cgctaacgct ataaatctaa
     4621 aaaacaaaaa actcaaacag aaccacacta tctctccaat ccctggtccc gatttttctg
     4681 ttggctactt cgcacttgtt ttaatctctt ttgttgttct tcatgctctt gtttatgatt
     4741 caaaaacttt tgagcgcctc tcacttcatc atcactagta aaatatcgtt ttaaaaattt
     4801 gcaattcatc ctgtccaata tactcccctt aacgtaacca atcaccccag ccaattcagg
     4861 aagcttttct ttatacaact ctttcactcg ttccaacgtt ttcttaaaga aatcattctc
     4921 ttccttcaat cctcgattct ctcttttcaa ttctgttatt tgcccctcta aaatacattt
     4981 ctctctatac agcccatctt tttcacgtgt aattacacct gattcgtttt taaggcgtct
     5041 aacatcttct ctaagggctt ccgacgacct agctaatact tttatacttt cgaagtcttc
     5101 cgaggtaatc tcgaccgttt tagagcgtat aaacccacct ttttctttaa ctgggatctc
     5161 atccacactt tttgcttgat ctaaagacat ttttaaatca tgaagacgac tatctattcc
     5221 cttcttttct tgctctagtt cttctatttc ttgttgtaaa gtcaccgctt taaaccgctg
     5281 cgtcgataaa tgttttctat cactagaaac accacgctct atatcaaatc ccttttcttt
     5341 cacatgttca tagattttat cttgtaaatc atgaaactct gatttgtttt taaaaaatga
     5401 atacgctgat agtttttgtt tttcattaac tggaaccata cccacatgca tatgaggtgt
     5461 tttttcatca acatgtacgg aagcatacac aaagttctct tctccatatc tccccttcaa
     5521 aaaacgaaga gactcttcaa agaatcgtct ctgttccact tgagataaag aattcatata
     5581 atccggacta gccgaaatca tgaacgaaca acaaacaaca gcgtctttcc gaattgcacg
     5641 attagataca acaccattat caattcgatt ttgaattaat ttcttataat ccactttttc
     5701 tttgtcatga atcagatcat aattctgttc cgttcgtgct tgaataatat ctgaattgga
     5761 atgactttct ttttcacgtt gattatgtat ttgtaatcct ttcacatctc tcatcttaaa
     5821 tttttgcata tggcaaatca cgaacttcga catgggcctc gctccttttt ttcgggggta
     5881 gttccatgta aacagtagca cactacagtt tactgcgtaa acacgtgtgc ccttcgggac
     5941 atttcggcta ccgccgagcc ataacaagca tggcaaaaaa aatcatgggc tttgcccaaa
     6001 cccacaaggg aaactcttcc ccttgacccc tttcaatcga actccccaac ccctcaatcc
     6061 ttgagggact ccctcgccgg ttggcatccc caaaacggct aacgttttgg agttccaaga
     6121 gggttctggg cacagtgggt tcgtccactc cttaatgctc acttcgttcc gcttaccgtt
     6181 ggacaaacat catacaacta cctctaactt tttacagttg acatccatgt attaaaaagt
     6241 ctctttttat attcaccacg actacataac atgtcccgct gccatgcaat acatcctcgc
     6301 caatcttccc tatttttttc agctactaac caacttttct taaaaactat taaccccaac
     6361 aatgacaaag ccattcccgc gtcctcgtaa aatgtcctcg ccaaccttca acatttttca
     6421 actcgccacc gatctatggg aattttcttg tagtgaaatg actacttcct ctacaaacat
     6481 aatttttttt gttttacagg aagagcttca actattctct atttttcaaa catcatcaaa
     6541 tctattgata caatcatatt ctttatgaga accactgtcg gaacagccac agaataaaac
     6601 gacaactatt cctagagcaa ttccaatatc gaatccatag aaaaatacac ctcagaacgc
     6661 tcagaatcca ttctaggaac ttctagttcc cttaagaaac ccaacatcct aaaatactta
     6721 aaaacgatta taagaccctt aaaaagcaaa aataaaaaaa gagcataagg aaaccgaatc
     6781 ccactttgca aaaaaagtgg tgaaataggc tgtgcctatt gggtttctat cactctattt
     6841 ttagggatgt atttctaccg tttgtatgcg acctatatgc gtttttcggc actttgtatg
     6901 tgtcccgtat gcgcctatat gcgcttggta tgcgcttaaa attaaaccta aaaaaactgg
     6961 gatgcgtttt ttcgggttgt agttcgtttt gagaccgttt aaagatggtt tgtgggtcgt
     7021 cttgaggctt ttgtagatcg ttttgatgcc cttttaatag ttacagcaaa tataacaaga
     7081 aaaaagtcca ttcttttgaa aagaatggac tttttttgaa acgaggaaaa attaacttgt
     7141 aaaccatttt acctgtacat aaccagtata atacaaaata agaataaggg ttagaatttt
     7201 ctgttattta gactaacatt atgtgttaaa tatttttttt agtaattaaa taagctatga
     7261 tagctgctac taacgctaat gctacaataa ttaaattttg gattatatat cctgtaaatg
     7321 agtaatcact tttaacaatc cattgataaa tatttagaga tagaaaaagt gttaccggaa
     7381 ataaaataaa atacatttgt tttaaaattg aacttttatc cataaattgc acacttacct
     7441 ccataataag ctaaagtagc cgctaaacct gctacgctac ttcctataaa gaatttaacg
     7501 cctattttag ccgcttcttt ccataatttt ttctcaagat aatatgccaa tccaccatta
     7561 aatgcggctt gtacagcatt tactccaaaa aagtcatata aagcatcttt catgcaactt
     7621 gtaaaagata aaccagcaat atcactttta tcttttttaa tttgttgttt aagtgcatct
     7681 aaaccaacat cattcccata tttcgcttct attttattaa aatcaaaatc aacaatttca
     7741 ccttgattat tatatatagc ggcttcttca tgcataaatt gaaacgcttc tgctaattca
     7801 ttaatgctct tttcatcttg accagaaacg agagactgtt tcgtttgtat tatattgttg
     7861 ttatttgtag ttgccgcaaa aacattattt gtcacaaatg ctacatttcc tgccaatatt
     7921 cctccgacta acaatactgg aatcattttt ttatatgttt ttctccaatt acttctctca
     7981 atactaattt ttttcataaa tttcatcccc ttaagtttat tattcaaaat agataataaa
     8041 cttaatttac atataattca atattttgtt aataatccta atatttaggt cgattgctaa
     8101 ttgttggaat ttgcttttaa atatcttttc catgtgcata ttgttgaata atatcatttt
     8161 tttctttagc atcatgtgtt tgaatggaat tattgaattg ggaaaagaaa atacctgttg
     8221 tacatgctac ccctattagt atcagactaa attttttcat catttcaatg actccctttc
     8281 taataagttt cttttagctt tgtaactttt ataaaaatat tcattagatt tatccttatt
     8341 cttttcttca taaaatctta tagccaattc ttcagtataa tcttgtatgt atttccatag
     8401 attttgtttt tcaaaatatg gaatcgcgtc taacatgact ttttctaatt gttcagtaga
     8461 taatttattg tttaatgctt gtaaaaataa gaaatgaatt ttatattctt ggttacaaac
     8521 atttagccct ttttcaatat atgtgttt
//
