LOCUS       NZ_CP116335             8398 bp    DNA     circular CON 01-MAR-2024
DEFINITION  Bacillus thuringiensis strain BLB1 plasmid pBLB1_8398, complete
            sequence.
ACCESSION   NZ_CP116335
VERSION     NZ_CP116335.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32746260
            Assembly: GCF_028356755.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 8398)
  AUTHORS   Fayad,N., Barssoum,R., Marsaud,N., Nasserdinne,R., Abdelmalek,N.,
            Teste,M.A., Pailler,V., Gautier,V., Belmonte,E., Aceves,C.,
            Cescut,J., Fillaudeau,L. and Kallassy Awad,M.
  TITLE     Complete genomes of two Bacillus thuringiensis serovar kurstaki
            strains isolated from Lebanon and Tunisia, highly toxic against
            lepidopteran larvae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8398)
  AUTHORS   Fayad,N., Barssoum,R., Marsaud,N., Nasserdinne,R., Abdelmalek,N.,
            Teste,M.A., Pailler,V., Gautier,V., Belmonte,E., Aceves,C.,
            Cescut,J., Fillaudeau,L. and Kallassy Awad,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2023) Faculty of Science, Saint Joseph University
            of Beirut, Mar Roukoz, Mansourieh, Beirut 1514, Lebanon
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. v2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: ASM71753v1
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/01/2024 02:57:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,608
            CDSs (total)                      :: 6,457
            Genes (coding)                    :: 6,143
            CDSs (with protein)               :: 6,143
            Genes (RNA)                       :: 151
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 314
            CDSs (without protein)            :: 314
            Pseudo Genes (ambiguous residues) :: 0 of 314
            Pseudo Genes (frameshifted)       :: 114 of 314
            Pseudo Genes (incomplete)         :: 220 of 314
            Pseudo Genes (internal stop)      :: 67 of 314
            Pseudo Genes (multiple problems)  :: 69 of 314
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116335.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8398
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="BLB1"
                     /isolation_source="soil sample"
                     /db_xref="taxon:1428"
                     /plasmid="pBLB1_8398"
                     /geo_loc_name="Tunisia"
                     /collection_date="2008-01-01"
     gene            995..1516
                     /locus_tag="PJW01_RS32960"
                     /old_locus_tag="PJW01_32960"
     CDS             995..1516
                     /locus_tag="PJW01_RS32960"
                     /old_locus_tag="PJW01_32960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3967 domain-containing protein"
                     /protein_id="WP_000234812.1"
                     /translation="MVIIYQTTKQAAQTLNINHTTFKKYYGMFERYTGYNFLRDSKGQV
                     MFSEYDLEVFKRLLLVKAEPGRTIEESCRLVGEEFGISDKNRVITDISPENGGDNKAIE
                     ELKELILMQNNKIDELTIKLNEQSNQTKMIETSVGDRDQQLVRLMKEMLEVKRMVAASN
                     KKRWWHFFRK"
                     /name="DUF3967 domain-containing protein"
     gene            1601..1885
                     /locus_tag="PJW01_RS32965"
                     /old_locus_tag="PJW01_32965"
     CDS             1601..1885
                     /locus_tag="PJW01_RS32965"
                     /old_locus_tag="PJW01_32965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000391901.1"
                     /translation="MEELNKKPQAEMVRVNTRISKEVNGWLDEKSARTGVPKSTLIYLA
                     LEQYIQQQTMFEELPKLQQMYTQIQQQQMVQGDLLSVVNKDNNLAKFSL"
                     /name="hypothetical protein"
     gene            1911..2153
                     /locus_tag="PJW01_RS32970"
                     /old_locus_tag="PJW01_32970"
     CDS             1911..2153
                     /locus_tag="PJW01_RS32970"
                     /old_locus_tag="PJW01_32970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000340717.1"
                     /translation="MDDALTKILEKLDQLDKKVDTIGSEVKEIRKEMQAHRMEIDDNFT
                     KLYKLVEDKDVEIDVLNKRVFKNETTVEKLKRQKH"
                     /name="hypothetical protein"
     gene            2224..2577
                     /locus_tag="PJW01_RS32975"
                     /old_locus_tag="PJW01_32975"
     CDS             2224..2577
                     /locus_tag="PJW01_RS32975"
                     /old_locus_tag="PJW01_32975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001179589.1"
                     /translation="MQNIKEIKKLIRKNPGKYASISTTEDGDFEGVLLSGFFAKQVMKL
                     ASQSNHISHDFNSNDLRSSDLTLKGVFLFNGTGVHFVFYEGLESIRIYDSPMFEEDSKE
                     MFEYFKEWLHAHS"
                     /name="hypothetical protein"
     gene            2717..2965
                     /locus_tag="PJW01_RS32980"
                     /old_locus_tag="PJW01_32980"
     CDS             2717..2965
                     /locus_tag="PJW01_RS32980"
                     /old_locus_tag="PJW01_32980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000364675.1"
                     /translation="MDLKTYYANVINELESILLSSEKVCEDNFLKIEQYLSNKSFIEND
                     IDYIFYHLDNLECLVKDRVFLEDALKRYREKLDKVKK"
                     /name="hypothetical protein"
     gene            3059..5431
                     /locus_tag="PJW01_RS32985"
                     /old_locus_tag="PJW01_32985"
     CDS             3059..5431
                     /locus_tag="PJW01_RS32985"
                     /old_locus_tag="PJW01_32985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_001162327.1"
                     /translation="MALDIAKCGRRTIVLREKATGDVMIRRMSCNQLSCPVCGPVIAAR
                     LQLRIKYFSQHERLFFFNTITSKDGIDDLEKTFKKIRKELSSDFTIEGYMRRKKVSYEK
                     ALKWYEKKKKKMIDFDIDVELGIYSRKNAIIEVANLKKVYYLGLNVKQKIEFHKKYNHL
                     VDELFPAIHEVNKRDEILHKQLYERILRRFEENEFEDFKFIRVVELHESGQPHYHFLCN
                     RYVSHYLMKKITVEGVSEVYDNAYIVDHAMERNKTLTYDTVDTDIVSFYVSKVTRYVTK
                     ETIETFSELEKEGDIAKKLISSSNCIKLFDEKDEEESKYKKLGVYDYQINATSYEVPKV
                     LENSVEDFIKFHSVMLRDQYAIDLLKFSKSLDLDDFINCNYVIHERLIQVSVAEYKNKL
                     STVFDETDLTKEQLELLKSFVENKITLLLGRAGTGKTYTLVNLLKKLKPNPRTTFILTY
                     TGKASVRIKELLSQHELTEYKPMTVHKACSSNWNSNFLKNEYNNLECEYLIIDEVGMIP
                     RDILMKVLLAVPSYTKIIFAGDDAQLPPVNDSSVIPELEGLDFINTIRLTKVFRSGDTV
                     LELAYKVLNRELIDFRLYDEKQLEKLVLPLVEQGYQILTNTKKMTKKINLIVQANKDKI
                     TKCFNDYCYEIGDRVMIIANDSSRRVYNGDTGKIVDYDDKGILIQLDFEDRQVFYRYLD
                     TVNIVPAYAFTVHKSQGSEYENVAVFLESQPKLNTNNLLYTGITRAKKNIKVYVPDEKV
                     LSTMINCVPEPVSNITLNNVFDLVNGILDIKKTSSMG"
                     /name="AAA family ATPase"
     gene            5570..5767
                     /locus_tag="PJW01_RS32990"
                     /old_locus_tag="PJW01_32990"
     CDS             5570..5767
                     /locus_tag="PJW01_RS32990"
                     /old_locus_tag="PJW01_32990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000274139.1"
                     /translation="MYGAYLDPEDRDRIMGSLSDEQQEYLRIFLKRGNQTAFSSVLARL
                     KGGESEEDAEHISTQWMFIE"
                     /name="hypothetical protein"
     gene            5849..6589
                     /locus_tag="PJW01_RS32995"
                     /old_locus_tag="PJW01_32995"
     CDS             5849..6589
                     /locus_tag="PJW01_RS32995"
                     /old_locus_tag="PJW01_32995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3895 domain-containing protein"
                     /protein_id="WP_000182379.1"
                     /translation="MTNKILKFSKDHFKKHTGLPSSIVKAVLKGMYKIDCELDEILIKI
                     SSGWSLFENLGMDSIPNDLIMPIDIREHLELDLPLLERQVMRLKGDLRSYNSLKKNIVE
                     KNDSVDFEINEEREEGQASLFDEDIVFGFNLDDDNVQTIRSQPVKVKPVSIEKNISYQF
                     LSFVEQEFILQFIQNVNSVSVRQLCEFMIKEVGSLDKRYLTGKPHIYVHVCSYLDFLVT
                     QGKLSYIENLDYIDRIYYIESDIS"
                     /name="DUF3895 domain-containing protein"
     gene            complement(6621..6761)
                     /locus_tag="PJW01_RS33000"
                     /old_locus_tag="PJW01_33000"
     CDS             complement(6621..6761)
                     /locus_tag="PJW01_RS33000"
                     /old_locus_tag="PJW01_33000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000130115.1"
                     /translation="MTDKKERVEMRIPQSILKKVDEYKEENGISTRTATILELIRKGLN
                     K"
                     /name="hypothetical protein"
     gene            6872..7123
                     /locus_tag="PJW01_RS33005"
                     /old_locus_tag="PJW01_33005"
     CDS             6872..7123
                     /locus_tag="PJW01_RS33005"
                     /old_locus_tag="PJW01_33005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000888627.1"
                     /translation="MLEDKFLESKIKKLTNQNVVVFTDDEIIEGVLLDKDTGEERLFEV
                     FRKIWGKNFAWFGEGIYIQCEDTVTFRDYDEVDNIFKK"
                     /name="hypothetical protein"
     Typing          1602..1885
                     /name="rep_cluster_2006"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.17e-147"
ORIGIN
        1 cacgattgaa aaagggaatg cgttttgtgg tcacaaaaca tttataaggt ctattttact
       61 aatgttttga tgtagcctta ctaatagatc cgacagatta aataattaat gtttgtggca
      121 attagcgagg aatggcgaag attagggaag acattttttg aagtatattg gggcgaagta
      181 ttaagatttt tatccaagta atcgttataa aaaaatcaaa atgaaatggc agcaggacta
      241 aattcaggat cgttggaggt ttaaatacga atatagtgtt ggggtctcaa gaagagataa
      301 gtatatattt agagattcta tagaaaaaat atttttaata tttgtcgcag aattagtgct
      361 tacgccctaa agattatgtt aggtatggcg cgcattcggc cttaggcgga tgcgcgccag
      421 ggatagttaa gcgttaagag gatgaaaaat attaacaagg tagttatcta tagagttatt
      481 taatatatag ttggatctaa ttgtgagtta tagaagatat agccgatata acttcagcta
      541 taaaggtaga atgattaaaa gaaggaacga tgtatatatt catcagaata cgcaaaatca
      601 cttctggaag gtttgaaatg tgaatcggta gatggaaatg gaaagcctgt aagaagccgt
      661 agcaaggcga aggagtttta tttagaaaag ttttttgatt tgtaaaaagt tttttaaata
      721 aaacttgatg tcccgtaggg ttgcctaaag gaatagtaaa tcaagctatt gcgtactccc
      781 aaacattgtt tgggggacac tcgctgtgag cgtaaagggt gtgggaatat cccacgaata
      841 aaaaattttt gtgtcccttt ttttaaaaag gacggtttga ttttgtggaa taatacggga
      901 cgtgaaattt tattatgatt tataaagttt atataaatta taaaacaagg agttatatcg
      961 gttatatcga atgtaaattt gtgaggtgat tgcaatggtt attatatatc aaacaacaaa
     1021 acaagcagca caaacattaa atattaatca tacaactttt aaaaaatatt atggcatgtt
     1081 tgagagatat accggatata acttcttaag agattcaaaa ggacaggtaa tgtttagtga
     1141 atatgattta gaagtattta agaggttatt attagttaag gcggaaccag gaaggactat
     1201 agaagaatcg tgtagacttg ttggtgaaga gtttggtatt tcggataaga atcgagttat
     1261 aaccgatata tctcctgaaa acggagggga taataaggcg attgaagagt taaaggaatt
     1321 gattttaatg cagaataata aaattgatga attaactatt aaattaaatg aacagtcaaa
     1381 tcaaactaag atgattgaaa caagcgttgg agatagagat cagcaattgg ttagattaat
     1441 gaaagaaatg ctagaagtga aaagaatggt ggcggcaagt aataaaaaaa gatggtggca
     1501 ttttttcaga aaataaattt agccgaaaag gcttttttta ttgttttata gactctattg
     1561 atgtaaaatt ggtgttaata aaacatcaag gaggatcaag atggaagaat taaataaaaa
     1621 accacaagct gaaatggttc gtgttaatac tcgtatttct aaagaagtta atggttggtt
     1681 agatgaaaaa agcgctagaa ctggtgtacc taaatctact ttaatttatt tggctttgga
     1741 acaatatata caacaacaaa caatgtttga ggagttacct aaacttcaac aaatgtatac
     1801 gcaaatacaa caacaacaaa tggttcaagg agatttgtta agtgtggtta ataaggataa
     1861 caatttagct aagtttagtt tgtaatttaa ttttaggagg agaattaaat atggatgatg
     1921 cattaacaaa aattttagag aaattggatc aacttgataa gaaagtagat acgattggta
     1981 gtgaggtaaa agaaattaga aaagaaatgc aagcacatcg aatggaaata gatgataatt
     2041 ttacgaagtt atataagcta gttgaggata aagatgttga gattgatgtt ctgaataaac
     2101 gtgtatttaa aaatgaaaca acagttgaaa aattaaagag acaaaaacat taggattcat
     2161 atgtgaatta aaattatatg tgtttatttt taaagaaaga agatatttaa ggggaatgat
     2221 atcttgcaaa atattaaaga gataaagaaa ttaattagga agaatcctgg taaatatgcg
     2281 agcatttcta caactgaaga tggtgatttt gaaggggttt tattgagtgg attttttgca
     2341 aagcaagtta tgaaattggc ttctcaatcc aatcatattt ctcatgattt taattcaaat
     2401 gatcttagat cttctgatct aactttaaaa ggagtttttc tttttaatgg tactggagtt
     2461 cattttgttt tttatgaggg tcttgagtct ataaggattt atgattcgcc aatgtttgaa
     2521 gaggattcta aagagatgtt tgagtatttt aaggagtggt tacatgcaca ttcttgatat
     2581 ttactttagt aaacgttgtg agaatgtaat aaaacatatt taacagttta ctgtgtaaat
     2641 gggcactttg tggaattgtt tatagagagg tgttgttatg gacactttgt ggaattgttt
     2701 atagagaggt gttgttatgg atttaaaaac gtattatgcg aatgttatta atgaactaga
     2761 aagtatttta ttgtcttcag aaaaggtttg tgaagacaat tttttaaaga ttgaacaata
     2821 tttaagtaat aaatctttta ttgaaaatga tattgactat attttttatc atttagataa
     2881 tcttgagtgt ttagtaaaag atcgtgtttt tcttgaagat gctttaaaaa gatatagaga
     2941 aaagttagat aaagtaaaga agtaaattca tagtttaatg tagtttctac ttaactagtt
     3001 aataataata gggatagagt cgcacttttt attcgttttc ttagaaaggt tgttttttat
     3061 ggctttagat attgctaaat gcggtcggcg gaccatcgtt ttacgcgaaa aagctactgg
     3121 tgatgttatg atccgaagaa tgtcctgtaa ccagctttct tgccctgtat gtggtccggt
     3181 cattgcagca cgtttacaac ttcgaattaa atatttttct cagcatgaac gtctattctt
     3241 ttttaatact attacttcta aggatggtat tgatgatctg gagaagactt ttaaaaagat
     3301 tcgtaaagaa ttgtctagtg attttacgat tgaaggatat atgagacgta aaaaagttag
     3361 ttacgagaaa gctttaaaat ggtatgaaaa gaaaaagaaa aaaatgatag attttgatat
     3421 tgatgttgaa cttggaattt attcaagaaa aaatgcaatc attgaagttg caaatttaaa
     3481 aaaggtttat tatttgggtt taaatgttaa acaaaaaatt gaatttcata agaaatataa
     3541 tcatttagta gacgagctat ttccggctat tcatgaagtt aataagcgag atgaaatatt
     3601 acataaacag ttgtacgaac gaattttaag acgttttgaa gagaatgaat ttgaagattt
     3661 taagtttatt agggtagttg agttgcatga aagtggacaa cctcattatc attttttatg
     3721 taatcgttat gtttctcatt atttaatgaa gaagataaca gttgaaggtg tgagtgaggt
     3781 ttatgataat gcgtatattg tggatcatgc tatggagaga aataaaactc ttacatatga
     3841 cactgtagat actgatattg tttcttttta tgtatcgaaa gttactagat atgttactaa
     3901 ggagactata gaaacctttt ctgaattgga aaaggaaggt gatatagcta agaaattaat
     3961 tagttcaagt aattgtatca agttgtttga tgaaaaagat gaagaagaat ctaagtataa
     4021 gaaattaggg gtatatgatt atcaaattaa tgcaacaagt tatgaggttc caaaagtatt
     4081 agaaaatagt gtagaagatt ttattaagtt tcattctgta atgcttaggg atcagtatgc
     4141 aattgattta ttgaaatttt caaagtcatt ggatttagat gattttataa attgtaacta
     4201 tgtaattcat gagcgtttaa ttcaagtttc agttgctgaa tataaaaata agttatcgac
     4261 tgtttttgat gagactgatt taactaaaga acaattagag ttattaaaat cttttgtaga
     4321 aaataaaatt acattattgt taggacgagc tggtacaggt aaaacttata ctttagttaa
     4381 tttgttgaaa aaattaaagc ctaatccacg tacaactttt attttaacgt atacaggtaa
     4441 agcgtctgta cgaattaaag aattgttgag tcaacatgaa ttaaccgaat acaagcctat
     4501 gacagttcat aaagcatgtt catctaattg gaattcgaat tttttaaaga atgagtataa
     4561 taacttagaa tgtgagtatt tgattattga tgaagtaggt atgataccaa gagatatttt
     4621 gatgaaagta ttattagctg ttcctagtta tacaaagatt atttttgctg gtgatgatgc
     4681 tcaacttcca cctgttaatg attcttctgt aattcctgaa ttagaaggtt tagattttat
     4741 aaatactata agacttacaa aagtttttag aagtggagat actgtgttag agcttgcata
     4801 taaagtttta aatcgtgaat taattgattt tagattatat gatgaaaaac aattagaaaa
     4861 actagtttta ccacttgtgg aacaggggta tcaaatttta acaaatacaa aaaaaatgac
     4921 taagaaaatt aatttaatag ttcaagctaa taaggataaa attacgaagt gttttaatga
     4981 ttattgttat gaaatcggtg atagagtcat gatcattgct aatgattcat ctagaagggt
     5041 ttataatggt gatactggta aaattgttga ttatgatgat aaaggtattt taattcaatt
     5101 ggattttgaa gataggcaag tgttttatag atatttagat acagttaata ttgtaccagc
     5161 ttatgcattt acagttcata aatctcaagg tagtgaatat gagaatgtgg cggtattttt
     5221 agaaagtcag ccgaaattaa atactaataa tttattatat acaggaatta caagagcgaa
     5281 gaaaaatata aaagtttatg tgccggatga aaaggtttta tcgactatga taaattgtgt
     5341 tccagaaccg gtttcgaata taacattaaa taatgttttt gatttagtaa atgggatttt
     5401 agatataaaa aaaacatctt caatgggctg aagatgtaca aaaatgtaga ttcttcaatg
     5461 ggttgaagaa tcataagact gaaatgattg ttaaagaatc taattgtatt atatatgtaa
     5521 tttgtagcat ggtctattta atttaatttt tcggaaggat ttgttgttta tgtatggtgc
     5581 ttatttagat ccagaagata gggatagaat aatgggtagt ttgtctgatg aacaacaaga
     5641 gtatttacgt atttttttga agagaggtaa tcagacagca ttctctagtg tgttagctag
     5701 attaaaaggt ggcgaatctg aagaggatgc tgaacatata tctactcaat ggatgtttat
     5761 tgagtagatt gatgcaggaa aagtttcgaa agatcatccg tgtgagtgtg gtcgtccttt
     5821 acgttatcaa tatattgtta agaatttagt gactaataaa attttaaagt ttagtaagga
     5881 tcactttaaa aaacatacag gtttaccttc tagtattgtt aaggcagttt tgaagggaat
     5941 gtataagatt gattgtgagt tagatgaaat tttgattaaa atttcatctg gttggtcttt
     6001 atttgagaac ttaggtatgg attctattcc taatgatttg attatgccta tagatattag
     6061 agagcattta gaattagatt tacctttatt agaacgtcag gttatgagat taaaaggtga
     6121 tttgagaagt tataattcat taaagaagaa tattgttgag aaaaatgatt ctgtagattt
     6181 tgaaataaac gaggaaagag aagaaggaca agcttctttg tttgatgaag acattgtttt
     6241 tggatttaat ttagatgatg ataatgttca aactatacga agtcagcctg tgaaagtgaa
     6301 acctgtttct attgagaaga acatttcgta tcaattttta agttttgttg aacaagaatt
     6361 tattttgcag tttatacaaa atgttaattc tgtaagtgtg agacaactat gtgaatttat
     6421 gattaaagag gttggctctt tagataaacg ttatcttact ggaaagcctc atatctacgt
     6481 tcatgtttgt agttatttag attttttagt cactcaaggc aagttgagct atattgagaa
     6541 tttagattat attgatcgta tttattatat agaatcagat attagttaga agggatatgt
     6601 atattatccc ttctattttt ttatttattt aatccttttc ttataagttc tagaattgtt
     6661 gctgttctag tagaaattcc gttttcttct ttgtattcgt caactttttt taatattgat
     6721 tgtgggattc tcatttctac tctttctttt ttatctgtca ttattatcac ctcttcttgt
     6781 attgtacgga aaaatatcgt ataatacaaa ttgtacggaa aaaagacgta tttttcacgt
     6841 acaaaaaatg aaaatcaaag gggattttat tatgttagaa gacaaatttt tagaatctaa
     6901 gattaaaaaa ttaactaatc aaaatgtagt ggtttttact gatgatgaga ttattgaagg
     6961 agtccttttg gataaggata ctggagaaga acgtttgttt gaagtttttc gtaagatttg
     7021 gggtaagaat ttcgcttggt ttggagaagg aatttatatt caatgtgaag atacagtgac
     7081 gtttcgtgat tatgatgagg tagataatat atttaagaaa tagttttata taaatttata
     7141 ttaaatgcag attatttata gaagagcata aggttttttt gctatcacta aaggtgacag
     7201 gttagacaaa gcctaacaaa aacgtgatat cataaatatg agaaaatcaa tggagtttca
     7261 atgtgtttcc aatgggtttt cactgtatat aatatgttat tagaaattgg tttttttgct
     7321 tttagattga taattcttgt gaaagataaa atgaaattcc atttataaat gttgtatatt
     7381 ttaatagttt tataggagtt tttaagaaga ggttgatgag gatcaatgca tagcagccat
     7441 gtgcattatt gtattagcga ataaaaaaaa cgatatgtta acgttaccca aaaggatagc
     7501 tttaacatat cgtttttttt atttttatgc ctactacggt aaggctacac caatttttta
     7561 gttccaacta aggtatggat actccaaaat taatcatcat tcttttgttt tatgttgttg
     7621 cgaaggtgaa cagaaaaaaa cttcgctgaa tttcgttttt attttaccac aaatttaatt
     7681 ttttggtgca tttttagtga ccactgagtg accactaagt gccaacaaat gaccatcaga
     7741 atgaaaaaat gaccacccag tgaccaccaa ttaataaata ggcgtcccag ttttcgtggt
     7801 cgtttagtgg tcatttttgg cgcgtacaaa acgcatactt ttaatttttt tcacatacaa
     7861 aacgcatata aaatgaccaa aaatgcatac agaacgcata tgagtaaaaa aagacgtccc
     7921 actttttcgg gttaaaaaaa cgttgattta aaggggcgta aagcgtgcta caagggacac
     7981 aaaacgtgcc acaaaagtgc tttaaacgta cacaaaacgg gctacaaagc ccaaaaaggc
     8041 atcccgtaat tcacaaaata gaaacccaat aggctcagcc tatttcatca ctttttttat
     8101 aaaatgggat tcggtttcct tatgctcttc tttaaaaaaa ggtttatatg gctttttaag
     8161 ctggtaaata ttttgtatta aaaaaagaaa atgcaggata aggagaggga cacagttgtg
     8221 tctacgtgta tcagtcaaag cctgatagaa taatttttca ttgtgtacag agttgtgacc
     8281 acgattgaaa aagggaatgc gttttgtggt cacaaaacat ttataaggtc tattttacta
     8341 atgttttgat gtagccttac taatagatcc gacagattaa ataattaatg tttgtggc
//
