LOCUS       NZ_CP116333             7792 bp    DNA     circular CON 01-MAR-2024
DEFINITION  Bacillus thuringiensis strain BLB1 plasmid pBLB1_7792, complete
            sequence.
ACCESSION   NZ_CP116333
VERSION     NZ_CP116333.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32746260
            Assembly: GCF_028356755.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 7792)
  AUTHORS   Fayad,N., Barssoum,R., Marsaud,N., Nasserdinne,R., Abdelmalek,N.,
            Teste,M.A., Pailler,V., Gautier,V., Belmonte,E., Aceves,C.,
            Cescut,J., Fillaudeau,L. and Kallassy Awad,M.
  TITLE     Complete genomes of two Bacillus thuringiensis serovar kurstaki
            strains isolated from Lebanon and Tunisia, highly toxic against
            lepidopteran larvae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7792)
  AUTHORS   Fayad,N., Barssoum,R., Marsaud,N., Nasserdinne,R., Abdelmalek,N.,
            Teste,M.A., Pailler,V., Gautier,V., Belmonte,E., Aceves,C.,
            Cescut,J., Fillaudeau,L. and Kallassy Awad,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2023) Faculty of Science, Saint Joseph University
            of Beirut, Mar Roukoz, Mansourieh, Beirut 1514, Lebanon
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. v2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: ASM71753v1
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/01/2024 02:57:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,608
            CDSs (total)                      :: 6,457
            Genes (coding)                    :: 6,143
            CDSs (with protein)               :: 6,143
            Genes (RNA)                       :: 151
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 314
            CDSs (without protein)            :: 314
            Pseudo Genes (ambiguous residues) :: 0 of 314
            Pseudo Genes (frameshifted)       :: 114 of 314
            Pseudo Genes (incomplete)         :: 220 of 314
            Pseudo Genes (internal stop)      :: 67 of 314
            Pseudo Genes (multiple problems)  :: 69 of 314
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116333.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7792
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="BLB1"
                     /isolation_source="soil sample"
                     /db_xref="taxon:1428"
                     /plasmid="pBLB1_7792"
                     /geo_loc_name="Tunisia"
                     /collection_date="2008-01-01"
     gene            164..364
                     /locus_tag="PJW01_RS32490"
                     /old_locus_tag="PJW01_32490"
     CDS             164..364
                     /locus_tag="PJW01_RS32490"
                     /old_locus_tag="PJW01_32490"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_139252680.1"
                     /translation="MNIRISVVLPYGYSYSDSNRKCDCINVFGVFERIEKAEALPVKQK
                     NMFVEEVVISLQENSHRSVGR"
                     /name="hypothetical protein"
     gene            775..1980
                     /gene="mobV"
                     /locus_tag="PJW01_RS32495"
                     /old_locus_tag="PJW01_32495"
     CDS             775..1980
                     /gene="mobV"
                     /locus_tag="PJW01_RS32495"
                     /old_locus_tag="PJW01_32495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033080.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000033078.1"
                     /translation="MSKFMVCHMQKFKMTDVKGLQIHNQREKESHSNSDIIQERTEQNY
                     DLIHDKRKVDYKKFIQNRIDNGVVSNRAIRKDAVVCCSFMISASPDYMNSLSQAEQRRF
                     FEESVCFLKARYGEENFVYASVHVDEKTPHMHVGMVPVNEKQKLSAYSFFKNKSELHDL
                     QDKIYEHVKEKGFDIERGVSSDRKHLSTQRFKAVSLQQEIEKLEQEKKEIDSRLYDLAS
                     SLDQAKSVDEIPVKEKGGFIRSKMVEIASEDFDSIKSLAKSSESLRNENRRLKNEKIKI
                     EREKDDLYKGQRFLERQVTDLKRENRGLKEANDFLKKTLERVKEMYKEKLPELAGVIGY
                     VKGSILDKMNRKFLKRHFAGDDEVKGAQKFLNHKQEHEEQQKRLKQVRRSQQKNWDQGL
                     ER"
                     /name="mobV"
     gene            2012..2293
                     /locus_tag="PJW01_RS32500"
                     /old_locus_tag="PJW01_32500"
     CDS             2012..2293
                     /locus_tag="PJW01_RS32500"
                     /old_locus_tag="PJW01_32500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002026684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_003273526.1"
                     /translation="MEYFHSYGVKFIGTVKELAKKNNLTIEETCRILELTMKIDDYDRK
                     DEQLAGIGELIKEMNDQIMGLSGGLRAISERLRDMNENVVLVSLEDKE"
                     /name="hypothetical protein"
     gene            complement(2455..2856)
                     /locus_tag="PJW01_RS32505"
                     /old_locus_tag="PJW01_32505"
     CDS             complement(2455..2856)
                     /locus_tag="PJW01_RS32505"
                     /old_locus_tag="PJW01_32505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158318.1"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="WP_000158319.1"
                     /translation="MTIYITETQAIFINETLIDMYSPNEQRGVKDTGLLQSAIYRPQQT
                     VAQEDAYPTIFHKATALFESLAKNHAFYNANKRTALACLEMFLLYNEYELKMSEQESSD
                     FTVNVVEQRLSFEEILKIIKENSSKLPLN"
                     /name="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(2894..3178)
                     /locus_tag="PJW01_RS32510"
                     /old_locus_tag="PJW01_32510"
     CDS             complement(2894..3178)
                     /locus_tag="PJW01_RS32510"
                     /old_locus_tag="PJW01_32510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126403.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WP_000126403.1"
                     /translation="MTANTRHMETQKIERKVTKIGNSLGLTLPQEFYTTLNIKQGDVVS
                     LEINEENEQIVIKKSKKISMPEDLNPKFLESLNRTINKFDGAFKDLVNR"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            4049..5092
                     /locus_tag="PJW01_RS32515"
                     /old_locus_tag="PJW01_32515"
     CDS             4049..5092
                     /locus_tag="PJW01_RS32515"
                     /old_locus_tag="PJW01_32515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162903.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_000162904.1"
                     /translation="MTKKNQDEITGKLQPKKKQNIRIYEFIEKKMSESGRELFKSCSTF
                     NEFVATKDKKKKKRVKGNDCKNRFCPICAWRKAGKDAVKIATMMEAIKIEEKKEFLFLT
                     LTTPNIKADTVKSEIDRFNKAFNKLFKRRNIQRSIKGYIRKLEMTYDKERFITEEMYNN
                     KKRKAYYDSRGLKVDDHNPNYDTYNPHFHVLLCVEKNYFKRKELYIKQEEWLEMWREVT
                     DMPEITQVHIQKVELIREGNAVAEVAKYSAKDYEMSVSQDVFDVFYLALKGRQLIVYGG
                     LLKDYAKKYEDGELDKYKSTDKNEYYYRLIAMWNKDLMKFEQEYQELTESEKQEYNGHL
                     IDEMEVE"
                     /name="protein rep"
     gene            complement(6097..6360)
                     /locus_tag="PJW01_RS32520"
                     /old_locus_tag="PJW01_32520"
     CDS             complement(6097..6360)
                     /locus_tag="PJW01_RS32520"
                     /old_locus_tag="PJW01_32520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000874966.1"
                     /translation="MAFLTNYKANGKRYFYVEKYVGKKPYTCKQSERIYSIGNERITLE
                     RLTLWILDNSFIPNELIKIGISIDDIENWREKVENTIKRYSL"
                     /name="hypothetical protein"
     gene            complement(6374..6661)
                     /locus_tag="PJW01_RS32525"
                     /old_locus_tag="PJW01_32525"
     CDS             complement(6374..6661)
                     /locus_tag="PJW01_RS32525"
                     /old_locus_tag="PJW01_32525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000323412.1"
                     /translation="MAYIISKKVKGNIYFYVAQYVGTQPYYSKQYKYKCIYAIGNQKIA
                     LERIAMWLLDNNRIPKELLEIGVSINDVKYWYEKVEKTLQNYSLTNHKNT"
                     /name="hypothetical protein"
     gene            7019..7246
                     /locus_tag="PJW01_RS32530"
                     /old_locus_tag="PJW01_32530"
     CDS             7019..7246
                     /locus_tag="PJW01_RS32530"
                     /old_locus_tag="PJW01_32530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869766.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000869766.1"
                     /translation="MKVVNNFRVIMAVKNIDNVAELVRMTGVSRNSINKLWRNDNVSSL
                     RIDTLLPICEKLNVKLSDLIEYIPDDSETK"
                     /name="helix-turn-helix transcriptional regulator"
     Typing          3915..5092
                     /name="rep_cluster_617"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          776..1977
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 catactaaac gtttttaaac gtatagagga cgcatacttt agggaaaatc gcttccctga
       61 agtagagcat gagaaaccca ataggcacag tctatttcac cactttttgt acaaagtggg
      121 attcggtttc cttatgctct tttttatttg aatgggtcta ggaatgaata tacgaatatc
      181 tgtagtttta ccctatggct attcttatag tgattctaat agaaaatgtg attgtattaa
      241 tgtttttggg gtgtttgaaa gaatagaaaa agctgaagct cttcctgtaa aacaaaaaaa
      301 tatgtttgta gaggaagtag ttatttcact acaagaaaat tcccatagat cggtggggcg
      361 gtgaatataa aaagagactt tttaatatat ggatgtcaga ttttaaaagg gggaggagta
      421 tttatggtac ctgtcaacgg taagcggacg gaatgaagcg atagcgaaat gatggagcat
      481 taaggagttg ataggtaact tcgaccaaga ccctcttgtt cttcccaaaa ccatgttttg
      541 gggatgccta ccggcgaggg agtccctcaa ggattgaggg gttggggagt tcgattgaaa
      601 ggggtcaagg ggaagagttt cccttgtggg tttgggcaaa gcccataatt cttttgccat
      661 gcctgtcatg gctcggcggt cgccgaaatg tcccgtaggg cacacgtgtt tacgcagtaa
      721 actgtagtgt gctacagttt acatggaact acgaccgaag aaggagcgat gcccatgtca
      781 aaattcatgg tttgtcatat gcaaaaattc aagatgacag atgtgaaagg attacaaata
      841 cataatcagc gtgaaaaaga aagtcattca aattcagaca ttattcaaga acgaacggaa
      901 cagaattatg atcttattca tgataaaaga aaagtggatt ataagaaatt cattcaaaat
      961 cgaattgata atggtgttgt atctaatcgt gcgattcgga aagatgctgt tgtttgttgt
     1021 tcgtttatga tttcggctag tcctgattat atgaattctt tatctcaagc ggaacagaga
     1081 cgattctttg aagagtctgt ttgtttttta aaggcgagat atggagaaga gaactttgta
     1141 tatgcttcag tacatgttga tgaaaaaaca cctcatatgc atgtgggcat ggttccggtt
     1201 aatgaaaaac aaaaactatc agcgtattca ttttttaaaa acaaatcaga gcttcatgat
     1261 ctacaagata aaatctatga acatgtgaag gaaaagggat ttgatataga gcgtggtgtt
     1321 tctagcgaca ggaaacattt atcgacgcag cggtttaaag cggtgagttt gcaacaagaa
     1381 atagagaagt tagagcagga aaagaaagaa atagatagtc gtctttatga tttagcatcg
     1441 tctttagatc aagcgaaaag tgtagatgag attccagtca aagaaaaagg cgggttcata
     1501 cgctctaaaa tggtcgagat tgcttcagaa gacttcgata gtataaaatc attagctaag
     1561 tcttctgagt ctcttagaaa cgaaaataga cgccttaaaa atgaaaagat taagattgaa
     1621 cgtgaaaaag atgatttata taaggggcaa cgttttttag aaaggcaagt aacagacttg
     1681 aaaagagaga atcgagggtt gaaggaagca aatgattttc ttaagaaaac gttggagcga
     1741 gtgaaagaga tgtataagga gaagcttcct gaactcgcag gggtgattgg ttatgttaag
     1801 gggagtatac tggacaagat gaatcgcaag tttttaaaac gacattttgc tggtgatgat
     1861 gaagtaaagg gtgctcaaaa gttcttgaat cacaaacaag agcatgaaga acaacaaaag
     1921 agattaaaac aagtacgaag aagccaacag aaaaattggg accagggatt ggagagatag
     1981 tgtgggaaca ttatttaatc aacaaccaag gatggaatat tttcatagtt atggagttaa
     2041 gtttataggt actgtaaagg aattggctaa gaaaaataat ttaacgattg aagagacatg
     2101 taggattttg gaattgacga tgaaaattga tgattatgat cgtaaggatg aacaattagc
     2161 tggaattgga gaacttatca aggaaatgaa tgatcaaatt atgggactta gtggaggtct
     2221 gagagctata agtgaacgtt tgcgtgatat gaatgaaaat gttgtattag tatctttgga
     2281 agataaagaa taatggcatg ttaggaggct ctgtgtgagt ctcttttttc tttagatgtg
     2341 tagcgttagc ggaacttgtt ttgcataata tagcgaggat tgtagagcct tgtataacat
     2401 ccttgaagat agtttgttta ataaaaaaga cgtggttaaa tcccacgtct ttttctaatt
     2461 taaaggtaat tttgatgagt tttcttttat aatttttaag atttcttcaa aagataatct
     2521 ctgttctact acattaacag tgaaatctga agattcttgt tcggacattt ttaattcgta
     2581 ctcattgtat aacagaaaca tttctaggca agctaatgcc gttcttttgt ttgcattata
     2641 gaatgcgtgg tttttagcca atgattcaaa taatgcagtg gctttgtgga agatagtcgg
     2701 atatgcatcc tcttgtgcta ctgtttgttg tggacggtat attgctgatt gtaataaccc
     2761 tgtatcttta acacctcttt gttcattagg tgaatacatg tcgataagtg tttcattgat
     2821 aaaaatagct tgagtttctg ttatataaat tgtcatttcg gttttcctac tttcctccac
     2881 gcctgaagga taattatctg tttaccaaat ctttaaatgc accgtcaaat ttattgatgg
     2941 ttctgtttaa cgattccaag aacttaggat tgagatcctc tggcatagaa atttttttgg
     3001 atttttttat aacgatttgt tcgttttctt cgttaatttc taaacttaca acgtcacctt
     3061 gtttgatatt tagagtagtg taaaattctt gcggtaatgt taaacctaaa gaattgccga
     3121 ttttagtaac tttacgttcg attttttgtg tttccatgtg gcgagtgttt gctgtcattt
     3181 ggacttcctc cttggaattt gccacactat tttttgtcgt agtctaattg ccactttgtg
     3241 tttgtattga gtttttcgaa ttatatagtg tgattttatg tttgttttgt ttgtttgctt
     3301 ttggtttttt cggtcttctt agttttctct tttgtttgtt gtgtttagtc tttctgtatt
     3361 attatgacgt ttagttagaa tgttataaca gttataacgt attaaccgtc aatacaatta
     3421 tactcatttt tagaaatatg taaacaaatt tatatttcat tgcaaaagat agggaagatt
     3481 gtagagcctt gcttgaattt gttttattta gacaaagaaa gtgtaaaggg agttacctgt
     3541 tattctaatt agcaggtaaa gttcgagaat ttctcgattt tttcatggca acacttcgaa
     3601 cgtgttctga aatatatgtt tttgaggtat aggggattga tgtgtaattc ttaaagagaa
     3661 aatattataa gttgtttgaa aaacgcttag aagtgaaaat gaagaggctg agagatagga
     3721 ataatttttg tttttaaaag aaaaaatgcc cttgtccacg aagtggattg aagggcattt
     3781 ttttatggtt tttcggacac gaaaaatacg gttttcttct ttctttaata cataggtaac
     3841 tatttccgca cggaaattga tgcctatgta ttcttttatc ccttgataca caagggattt
     3901 cgaaaaaaaa gacttgctat ttccggcggg tagatttatc atttttagtg tcgaattaaa
     3961 aacaataact accaaggaag gagcaagtcc gaaaaaggga ttcgtgtacc cttttttaag
     4021 caattcatat aggactaata tatcaaacat gacaaagaaa aatcaagatg aaatcactgg
     4081 taaattacaa cctaaaaaga aacagaatat aagaatttat gaatttatag agaagaaaat
     4141 gagtgagagc gggagagaat tgtttaaatc gtgcagtact tttaatgagt ttgtggctac
     4201 gaaagataaa aagaaaaaga aacgagtaaa gggtaatgat tgtaaaaatc gattttgtcc
     4261 gatttgtgct tggagaaaag cagggaagga cgcggtgaaa atcgcaacaa tgatggaagc
     4321 aatcaagatt gaagaaaaga aagagtttct tttcttaaca ttgacaactc cgaatattaa
     4381 agctgatacg gtaaaaagcg agattgatag atttaataaa gcttttaata aattatttaa
     4441 gagacgtaat attcaacgtt ctattaaggg ttatattaga aagctagaaa tgacgtatga
     4501 taaagaacga ttcatcactg aagaaatgta taacaacaaa aagagaaaag catattatga
     4561 ttctcgtgga ttgaaagtag acgatcataa cccgaattat gatacatata atcctcattt
     4621 ccatgtatta ctttgtgttg aaaaaaatta ttttaaacgt aaagagcttt atattaagca
     4681 ggaagaatgg ttggaaatgt ggcgagaagt aacagatatg ccagagatta cgcaagtaca
     4741 tattcaaaaa gtagagttaa ttcgagaagg aaatgcagtt gcagaggttg cgaagtattc
     4801 agcaaaagat tatgaaatgt cagtgagtca agatgtgttc gacgtgtttt atttagcttt
     4861 aaaaggtcga caattaattg tgtacggtgg attgctaaaa gattatgcta aaaaatacga
     4921 agatggcgaa ttggacaagt ataagagtac agataaaaat gagtattatt atagattaat
     4981 tgcaatgtgg aataaggatt taatgaaatt tgaacaagaa tatcaagagt taacggaaag
     5041 tgagaaacaa gaatataacg gtcatttgat tgatgaaatg gaggttgaat agttttgtat
     5101 aaaaaaggtg atattgttaa acatacctgt tgggaatgtt ataacgaaga aatgaaaatt
     5161 tatcggattt caaaaagtaa ggtcaaagat ggtgaagatg tatattactc atataagtgc
     5221 taggttaaat gtgaaaaatg tggtgcggtt gatgatattt gaatgacaaa gagagaacca
     5281 gtcttaatat taagtttggt tctctctttt ttgtattgaa agaaaacctt ctgagtgcag
     5341 caagtagcat tcaaaaggtt tttatgatct aacagagaat taacaaaaaa gagttttttc
     5401 ttaaatttaa atttgtgaat ttgtgaattt atttttaaag tttatttcga gatatatttt
     5461 gttctgatga tttcgtttta ctactgtacg agttaaaaac ctatagtgtg cgcatgaatt
     5521 tttttagata tatctatatc gataaaatac cgcacgtttt ttgatgtttt ttgaaaatag
     5581 aaattctaca tttttggggt tgattttgga tttttggtgt gggattttat ttaaaaattt
     5641 ttaaatttga aatcttgatt tttcgatttt tgaaattttt gttttccgtt ttctctgtaa
     5701 ttctgtattc cgaaattagg gagtttatat agtacctcta tgacgaactg aaaacgaaaa
     5761 aactttgctc gcatatttaa ttcatagata tttcatatcg ttaaaaagtt gcaccgtttt
     5821 aaacaagttg tgtgataaaa aaagatgttt agattatgtt tgaatttgtt gtttctgtga
     5881 attttttatt tcgtgattcc tgtatatttt taatcctttt tactcttaaa atctttttgc
     5941 ttttgtgcgc aaatgaaaaa ttcaaaaagc cacgcgtaac ctttattaaa tatataaata
     6001 tattattaaa atacctaacc cttttatgat gaaaaaatat aaatagttaa agaaaaaaga
     6061 ctgatgaaaa tatcagtctt ttttctttag ttttatttat aaagagtatc tctttatggt
     6121 gttttctact ttttctctcc agttctctat atcgtcaatt gatattccga tctttattaa
     6181 ttcgtttgga atgaaactgt tatctaaaat ccataaagtg agcctttcta atgtgattct
     6241 ttcgtttcca atcgaataaa tacgttcact ttgtttgcag gtatatggtt ttttaccaac
     6301 atatttttct acataaaagt atctttttcc gtttgctttg taatttgtta gaaatgccat
     6361 agtaatgctc ctttcaggtg tttttatggt tagttaaaga gtagttctgt aatgtttttt
     6421 caactttttc ataccaatat ttcacatcgt ttatggatac tccgatttct agtagttctt
     6481 ttggtatacg gttgttatct aatagccaca ttgcgattct ttctaaagct attttttgat
     6541 tgccgattgc gtaaatgcat ttatatttgt attgctttga gtaatatggt tgtgttccaa
     6601 cgtattgggc tacataaaag taaatgtttc cttttacttt ttttgatatt atatatgcca
     6661 aagaaatgac ctccaatcat taaaaatcta tattactttg attaatatta actttatcag
     6721 gttaattttt caacaaataa agtatgaaat aatgcatatt ttgaacgttt taatttaaat
     6781 aggttttaaa ttagtttaaa taaataaaat aaataacctt atatgtatat aaattaactt
     6841 taatttaaat gaagtatgaa atagtgcata atatgatttt ttattccata caggtttaaa
     6901 aatgttttat aaagtgctaa agttgtgttt aaaaggttaa aaaacaacta ttctttgcac
     6961 aaaagtgccc ttataagaaa aaagcatgta tatttcatga gaaaaaggag tataaaaaat
     7021 gaaagtagta aataacttcc gtgtgattat ggcggtaaag aatatagata atgttgcaga
     7081 gttggttagg atgacagggg taagtcgtaa ctctatcaat aagttgtggc gtaacgacaa
     7141 tgtgtcatct ttacgaatag atacattgct accgatctgt gaaaagttaa atgttaaact
     7201 atcggattta attgaatata ttccagacga tagtgagaca aaataattta ggggctattc
     7261 tatctttaga tagcttgtgt atgatgaaat cggtattaat atttgctagt ggaaaacagg
     7321 ttatattcgc atatggtttt tggtagggtt atatgcttat tttaaatttc ggcaaacttt
     7381 tatgactgtc tttttctttc tttttctgtt tgttatcaca tgaatatggt aaatgaaatt
     7441 ataggcttgc taggtgcgtc aacatcttgg aagatgagag gtaggcagat atttcgtgat
     7501 tttgtttaac tttatgtgtg attatcgtaa tgcagtaaaa tgaaatggtg gaattgatga
     7561 gtaatgaaaa aagagttcct atttgtgggg ctcttttttt atttgtcatg gtgtatgcaa
     7621 agcacatgca gaatacatac taaacgtttt taaacgtata gaggacgcat actttaggga
     7681 aaatcgcttc cctgaagtag agcatgagaa acccaatagg cacagtctat ttcaccactt
     7741 tttgtacaaa gtgggattcg gtttccttat gctctttttt atttgaatgg gt
//
