LOCUS       NZ_CP116326            11521 bp    DNA     circular CON 01-MAR-2024
DEFINITION  Bacillus thuringiensis strain BLB1 plasmid pBLB1_12, complete
            sequence.
ACCESSION   NZ_CP116326
VERSION     NZ_CP116326.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32746260
            Assembly: GCF_028356755.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Fayad,N., Barssoum,R., Marsaud,N., Nasserdinne,R., Abdelmalek,N.,
            Teste,M.A., Pailler,V., Gautier,V., Belmonte,E., Aceves,C.,
            Cescut,J., Fillaudeau,L. and Kallassy Awad,M.
  TITLE     Complete genomes of two Bacillus thuringiensis serovar kurstaki
            strains isolated from Lebanon and Tunisia, highly toxic against
            lepidopteran larvae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11521)
  AUTHORS   Fayad,N., Barssoum,R., Marsaud,N., Nasserdinne,R., Abdelmalek,N.,
            Teste,M.A., Pailler,V., Gautier,V., Belmonte,E., Aceves,C.,
            Cescut,J., Fillaudeau,L. and Kallassy Awad,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2023) Faculty of Science, Saint Joseph University
            of Beirut, Mar Roukoz, Mansourieh, Beirut 1514, Lebanon
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. v2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: ASM71753v1
            Genome Coverage                   :: 20.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/01/2024 02:57:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,608
            CDSs (total)                      :: 6,457
            Genes (coding)                    :: 6,143
            CDSs (with protein)               :: 6,143
            Genes (RNA)                       :: 151
            rRNAs                             :: 13, 13, 13 (5S, 16S, 23S)
            complete rRNAs                    :: 13, 13, 13 (5S, 16S, 23S)
            tRNAs                             :: 107
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 314
            CDSs (without protein)            :: 314
            Pseudo Genes (ambiguous residues) :: 0 of 314
            Pseudo Genes (frameshifted)       :: 114 of 314
            Pseudo Genes (incomplete)         :: 220 of 314
            Pseudo Genes (internal stop)      :: 67 of 314
            Pseudo Genes (multiple problems)  :: 69 of 314
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116326.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11521
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="BLB1"
                     /isolation_source="soil sample"
                     /db_xref="taxon:1428"
                     /plasmid="pBLB1_12"
                     /geo_loc_name="Tunisia"
                     /collection_date="2008-01-01"
     gene            complement(join(11002..11521,1..3018))
                     /locus_tag="PJW01_RS29685"
                     /old_locus_tag="PJW01_29685"
                     /pseudo=""
     CDS             complement(join(11002..11521,1..3018))
                     /locus_tag="PJW01_RS29685"
                     /old_locus_tag="PJW01_29685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369823.1"
                     /GO_function="GO:0090729 - toxin activity [Evidence IEA]"
                     /GO_process="GO:0001907 - killing by symbiont of host cells
                     [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="insecticidal delta-endotoxin Cry8Ea1 family
                     protein"
                     /name="insecticidal delta-endotoxin Cry8Ea1 family protein"
     gene            complement(3308..4003)
                     /locus_tag="PJW01_RS29690"
                     /old_locus_tag="PJW01_29690"
     CDS             complement(3308..4003)
                     /locus_tag="PJW01_RS29690"
                     /old_locus_tag="PJW01_29690"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003308398.1"
                     /GO_function="GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA]"
                     /GO_process="GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="WP_277662103.1"
                     /translation="MHAGDGNGAGNRSSIGIEICENRDGNFEKALTHAAGLVKFLMAHL
                     GLKIGNVVSHQHWSGKNCPRPIFNRPGGFEGFKRMIEDSLNDMVVENHNKDAGTQGLGI
                     AYMEGVNINLRRGPSTSSDVIRKLNKPESYIVWEEREGWLNLGNSWVKYDSSYIYFARR
                     QTSNIGKLVVVDTNELWVYGSANWNDKIKTVKKGEAFTIQDELQVNGSNMYKCKYFYIT
                     TNSQFVYVK"
                     /name="N-acetylmuramoyl-L-alanine amidase"
     gene            complement(4210..>4302)
                     /locus_tag="PJW01_RS29695"
                     /old_locus_tag="PJW01_29695"
                     /pseudo=""
     CDS             complement(4210..>4302)
                     /locus_tag="PJW01_RS29695"
                     /old_locus_tag="PJW01_29695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(5244..6104)
                     /locus_tag="PJW01_RS29700"
                     /old_locus_tag="PJW01_29700"
     CDS             complement(5244..6104)
                     /locus_tag="PJW01_RS29700"
                     /old_locus_tag="PJW01_29700"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-116"
                     /protein_id="WP_000027050.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-116"
     gene            complement(6255..6992)
                     /gene="erm(B)"
                     /locus_tag="PJW01_RS29705"
                     /old_locus_tag="PJW01_29705"
     ARG             complement(6259..6992)
                     /name="erm(B)"
                     /gene="erm(B)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="99.59"
                     /coverage="100.0"
     gene            complement(7117..7200)
                     /locus_tag="PJW01_RS29710"
                     /old_locus_tag="PJW01_29710"
     CDS             complement(7117..7200)
                     /locus_tag="PJW01_RS29710"
                     /old_locus_tag="PJW01_29710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /GO_process="GO:0046677 - response to antibiotic [Evidence
                     IEA]; GO:0031556 - transcriptional attenuation by ribosome
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="WP_001814874.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
                     /name="23S rRNA methyltransferase attenuation leader
                     peptide"
     gene            complement(7300..7671)
                     /locus_tag="PJW01_RS29715"
                     /old_locus_tag="PJW01_29715"
     CDS             complement(7300..7671)
                     /locus_tag="PJW01_RS29715"
                     /old_locus_tag="PJW01_29715"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_277662102.1"
                     /translation="MLGILFTAAPSTDVVKDLQDKVISLQDHEVAFLNTTISNMLTAVG
                     IGVAIITAVFTAAFAYVTYSNKRAKKNMDEASRKLEEAESKVSVLEEKSAQLERKILEA
                     EQLLADANSISNVGSPKQT"
                     /name="hypothetical protein"
     gene            7999..8403
                     /locus_tag="PJW01_RS29720"
                     /old_locus_tag="PJW01_29720"
     CDS             7999..8403
                     /locus_tag="PJW01_RS29720"
                     /old_locus_tag="PJW01_29720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBECR2 nuclease fold domain-containing protein"
                     /protein_id="WP_000126335.1"
                     /translation="MTANKKKIVNKPTVVAKLPQIIIDTYNLTCTSNDVKMFPGFIKHV
                     KKQHPGIYEKYSSHIKDIVEHPDYVGQNPKEPNSVELVKILNDHILIAIKLDPSGYLFL
                     STMFDLKNGPAKIQRRLNSGRLIAYKDLLS"
                     /name="PBECR2 nuclease fold domain-containing protein"
     gene            complement(8818..8994)
                     /locus_tag="PJW01_RS29725"
                     /old_locus_tag="PJW01_29725"
     CDS             complement(8818..8994)
                     /locus_tag="PJW01_RS29725"
                     /old_locus_tag="PJW01_29725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003319293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_003319293.1"
                     /translation="MRKEMAREQRQRFIDLLLDFMVKNPHLFVNGTEDESNNVVTKCNS
                     DIKEVAESYLTLL"
                     /name="hypothetical protein"
     gene            complement(<10610..10768)
                     /locus_tag="PJW01_RS29730"
                     /old_locus_tag="PJW01_29730"
                     /pseudo=""
     CDS             complement(<10610..10768)
                     /locus_tag="PJW01_RS29730"
                     /old_locus_tag="PJW01_29730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     ARG             complement(5248..6104)
                     /name="blaTEM-116"
                     /gene="blaTEM-116"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-116"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(6256..6992)
                     /name="ErmB"
                     /gene="ErmB"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="98.78"
                     /coverage="98.79"
     Typing          4299..4810
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.127"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaccgaacgt tggttgtttt gttcttctac atctacatgc cctttcacgt tccagcagga
       61 taagccatta ttaaaatcac catttttaat gacatttctc gcatcatata gggagaatgc
      121 agtgaaaata cgcccttcta attcttcaaa aatagccgca ttgacacccg gaatcacaga
      181 cagctcaggc agataagctt ctcgaatgct atgaacacgt ttatctgccg catgaatcat
      241 ggcaatattc gtatccgctt gtaattgatc atattgagag tttacaaata aagcatctac
      301 agattctttt gcctctttat aaacgatatt tgtttcccat tccaattttt cacgtttgtc
      361 tctccatttt ttctccgctc ttttcacacg agctagcgct tctcctacta atggtttctc
      421 ttcgagaaac tctagattcc ctagtcttgc gtgcccatct tgcgtcttaa tcttaaagat
      481 cacccataca cctaggtcct catttaagtc tgtacatcct acatcaatgt ctaaggagaa
      541 atgatgcgaa tgatgggcac acttttctcc atccctacac gaacaatcta agtcaggatt
      601 ccattcaagg tgtggcgcgc atcgattcgg ctctccacac tttccgattg gactttgggc
      661 tgaaagcggc cataaggaac ccgtacctgg cacatttact gtttcatgtt ttgcattgta
      721 gcgaattaaa tagatttcta agtcttgact atcttcgata taccctctta attgataacg
      781 ggtataggct tttaatttcg actcatctat tttttgatat aaatacgttg gatagcactc
      841 atcaaaggta cccaatagcg taacgtaatt ctctttgaat acgtcatcgc ctccttggat
      901 ggtaatatcc gtacttcctc tccagccacg gtctagttgt ctattgatcc ctctgaagtt
      961 tggatcttga agtaaattcc gctcatcact aagtcgcttc gcatgtttga ctttctcgga
     1021 caattcttgt ttttcatcca gacaaaattc atctgataaa cactcaacta aattggatac
     1081 ttgatcaata tgataatccg tcacatctgt ttttaacccg atttgattgg aagaagtaaa
     1141 cagctcattc accgcctttt gtgctctttc taaatcatat tctgcctcaa aggttacttc
     1201 tgccggaaca aattcaattc gatctatata aacttcattg cctgaattga agacatgagc
     1261 acttaacgta aatacacttg atccatttga aaagttaaac ggagtagtaa aacctacagt
     1321 cctaaagctt ccggactgta aattactccc actactcata gttgctgaaa aattaccctg
     1381 attaataggt cttccgtcaa ttgatgtatg gaattgtaaa tttgtagtag aagcgtagcg
     1441 aattcttacc cgatatcttt gtgataatgg tgcagtaata tttactctta aggttgaaat
     1501 ctggccaggt gaagttcttc gaagaatatc tcctcctgta aatcctggtc ctttaacgac
     1561 agaagttcca gagccaagat tagtagattt tgttaaaggt atttgtgtaa tttgtgatga
     1621 aggaattata ttattaaatt cagcactgcg atgctgccaa gaaaacgttg gagctctcaa
     1681 ggtgtaaact gctccagctg cttggctcag cattgtaaca tgactcaatc gatggctaaa
     1741 tcccgcacga ggtggtacac tattatcctg tggcggtatt acatctagtg aatcgactgt
     1801 acccctttgt ctatatatag tggaaggcaa gttggtcgtt agggaggcaa aagaaaactc
     1861 cgttccatca aggacaaaca gttcctgatt atttgggcct gaaccaagta taattcttct
     1921 atataaaggt gaagataatg ttctaaaaat ccccaaacca gttaatgaga caagtacggg
     1981 tggagctgca ttccccgcat tcccaaataa agggaatgcg aattctggtc ctgaaaaccc
     2041 tacaggagaa gctgttattt gatgccctga ccaataatta aagcctctat gcacatcagt
     2101 ataaatggtt atactattaa ggatatccat aagatgtggt tgcctaatat tctgttctat
     2161 tctctgagcc attccacgaa aactaccatc aaaattttct aatactgggt tcgtataaat
     2221 ttctcttgtt aattgggaaa ctgttcgaat tggatacctt cgactatcat aatttgagaa
     2281 tagagcaacg atatctaata cagtaagtgt tagctctctt ctaaattgat tataccttac
     2341 ccaatctcta gaatccggtc cccatacacg ctctaatccc gtattgtacc agcgcacagc
     2401 ataatctgta tagttgccaa taagcctagt taaatcatta taacgactat tgatagtcgc
     2461 ggcatcaaat ccccaccttt gtccaaacac tgaaacatct ctcaaaactg ataaatgtaa
     2521 atttgcagct tgaacatata ctgataaaag aggaacttga taattttgaa ctgccaaaag
     2581 aggaatagcg gttgtaaggg cactgttcat gtcattgaat tgaatacgca tctcttctct
     2641 taatgctgga ttagtaggat ctgcttccca ctctctaaaa gattctgcgt aaatttgata
     2701 aagattgctt agtccttcta atctagaaat ggcttggttc ctagcgaatt cttctattct
     2761 ttggttaatt aactgttcaa tttgtacaag aaatgcgtcc cattgagagg gaccaaaaat
     2821 tccccatatt atatcaacta gtcctaacac aaatccagca ccgggaacaa attcactcaa
     2881 aagaaattgc gttagcgaca aggaaatatc gattggggtg taaccagttt ctattctttc
     2941 tccacctaat acttctactt cagggttact taaacaatta taaggaatgc attcattgat
     3001 gttcggattg ttatccataa gttacctcca tctcttttat taagatacca attcattatg
     3061 atataatact gtttttcatt actacaattt aaaacatatg actcatctta tgaaaaaatg
     3121 cacaaagtgc aactaatttt actaaatatc aatttttgac ccagggtgtt aacaatctac
     3181 ttacttctcg cccattatcc atgctttaga ctttacaatg tttaaccaat gcattaaggg
     3241 tttgtattcc gttaacttat tttaaagtct tattactttt tatacttata tataaaatcc
     3301 acttttccta ttttacatat acaaattgag aatttgtggt aatgtagaaa tacttacact
     3361 tatacatgtt agaaccattt acctgcaatt catcttgaat tgtaaatgct tccccttttt
     3421 ttacagtctt aatcttatca ttccaattag cagaaccata gacccataat tcgtttgtat
     3481 ctacaaccac taatttacca atattactcg tttgtctacg ggcaaagtaa atataggaag
     3541 agtcatattt cacccatgaa ttacctaggt tcagccagcc ctcacgttct tcccaaacaa
     3601 tataagattc aggtttgttc aatttacgaa ttacatcact actcgtgcta ggtcctctgc
     3661 gaaggttaat atttacacct tccatgtaag caattcctaa tccttgtgta cccgcatcct
     3721 tgttatgatt ctctacaacc atatcgttta gactatcttc aatcatcctc ttgaaaccct
     3781 caaatccacc tggtcgatta aatatcggac gtggacagtt ctttccactc caatgttgat
     3841 gggaaactac gttcccaatc tttaatccaa ggtgagccat gagaaactta acgagacccg
     3901 cagcatgggt taaggctttt tcaaaattac catcacgatt ttcacaaatt tcaatgccga
     3961 tagacgagcg attacccgct ccattcccgt ctccggcatg catcgataga tctcgagggg
     4021 gggcccggta ccagcttttg ttccctttag tgagggttaa tttcgagctt ggcgtaatca
     4081 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     4141 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     4201 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     4261 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     4321 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     4381 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     4441 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     4501 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     4561 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     4621 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     4681 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4741 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4801 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     4861 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     4921 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     4981 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     5041 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     5101 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     5161 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     5221 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     5281 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     5341 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     5401 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     5461 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     5521 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     5581 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     5641 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     5701 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     5761 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     5821 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     5881 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     5941 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     6001 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     6061 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     6121 tgtaccccaa attccccgta ggcgctaggg acctctttag cttcttggaa gctgtcagta
     6181 gtatatctaa taatttatct ccattccctt tagtaacgtg taactttcca aatttaaaaa
     6241 agcgactcat agaattattt cctcccgtta aataatagat aactattaaa aatagacaat
     6301 acttgctcat aagtaatggt acttaaattg tttactttgg cgtgtttcat tgcttgatga
     6361 aactgatttt tagtaaacag ttgacgatat tctcgattga cccattttga aacaaagtac
     6421 gtatatagct tccaatattt atctggaaca tctgtggtat ggcgggtaag ttttattaag
     6481 acactgttta cttttggttt aggatgaaag cattccgctg gcagcttaag caattgctga
     6541 atcgagactt gagtgtgcaa gagcaaccct agtgttcggt gaatatccaa ggtacgcttg
     6601 tagaatcctt cttcaacaat cagatagatg tcagacgcat ggctttcaaa aaccactttt
     6661 ttaataattt gtgtgcttaa atggtaagga atactcccaa caattttata cctctgtttg
     6721 ttagggaatt gaaactgtag aatatcttgg tgaattaaag tgacacgaat gttcagtttt
     6781 aatttttctg acgataagtt gaatagatga ctgtctaatt caatagacgt tacctgttta
     6841 cttattttag ccagtttcgt cgttaaatgc cctttacctg ttccaatttc gtaaacggta
     6901 tcggtttctt ttaaattcaa ttgttttatt atttggttga gtactttttc actcgttaaa
     6961 aagttttgag aatattttat atttttgttc atgtaattac tcctgaagtg attacatctg
     7021 taaataaata cagaagttaa acgatttgtt tgtaatttta gttatctgtt taaaaagtca
     7081 taagattagt cactggtagg aattaatcta acgtatttat ttatctgcgt aatcactgtt
     7141 tttagtctgt ttcaaaacag tagatgtttt atctacatta cgcatttgga ataccaacat
     7201 gacgaatccc tccttcttaa ttacaaattt ttagcatcta atttaacttc aattcctatt
     7261 atacacaaaa ttttaagata ctgcactatc aacacactct taagtttgct tcggggatcc
     7321 cacattagaa atagaattgg catcagctaa gagttgttca gcttcaagaa tcttcctctc
     7381 caattgagcg cttttctcct ctagcacaga aactttactt tctgcttctt ctaattttct
     7441 actagcctcg tccatattct ttttagcacg cttattagaa tatgtaacat aagcaaacgc
     7501 tgctgtaaaa accgccgtta taattgccac tccaatacct actgctgtta acatattaga
     7561 tatcgtggtg ttcaaaaacg ctacctcatg atcctgcaat gatataactt tatcttgcaa
     7621 atcctttacg acatcagtag atggcgccgc tgtaaatagt atccccagca tcaatagttc
     7681 cccctgatta tataaattca gacgaatact attatccatt atttttttat taaggtaaat
     7741 taatatttta cctattaaag gagggacgat ttaagtatgg acgtgatgtt tggtcaaggt
     7801 gggaacataa attttaatta cattggaaca tattgtggac aagcatctaa ttcataatcc
     7861 ccactttcca agcatgaaag tcccttatac catcaattat tcaataaaat atccctatca
     7921 taaacctgtc ccgcgcgacg gttcaaatac tcctttatga taaaataaag atataaagag
     7981 acgagatgag ggagaaattt gacggctaat aaaaaaaaga ttgtaaataa accaactgtt
     8041 gtggcaaaac ttccacaaat cattatagat acatataact tgacttgcac ttctaacgat
     8101 gtaaaaatgt ttcccggatt tataaagcac gtaaagaaac aacatcctgg tatttatgaa
     8161 aaatattcat cgcacataaa agatatcgtt gaacaccccg actatgtcgg gcaaaatcct
     8221 aaagaaccta atagcgttga attggtaaag attttaaatg accatatact aattgctata
     8281 aagttggacc caagcggtta tttgttctta tcaactatgt tcgatctaaa aaacggtcct
     8341 gcgaaaatcc aaagacgatt gaatagtgga cgtttaattg cttacaaaga cctgttaagc
     8401 taacggggag tttactaaaa tataagaaca tggtatacta cctgtagaaa gtaacataat
     8461 aatgatatag gatctttgag gtgggaaagg ttccccacgc gcttatctaa ccgccgttct
     8521 caagcatttg attggctagg taagttacca gagatgtagg atacgccgcc ctaccaacga
     8581 tcctatgtac ggtaagccat ccgtttttta cggatggctt tttatattga ctttttcatc
     8641 gctaatggtt acttttctat caaacgacac cgaaaggcga tgattattgt gttaaaaaac
     8701 ccaatattat aacatccact aatggataat cttactttta gtaagtaggt tgcctagtga
     8761 aatacaataa ttccaacatt gggccttaat acatattaat tagttttaac cctctcacta
     8821 taaaagagtt aaatatgact ccgcaacctc tttaatatca ctattacatt ttgtaacaac
     8881 attattactt tcatcctctg taccattaac aaataaatga ggattcttta ccataaagtc
     8941 taacaataga tcaataaacc gctgcctttg ttctctagcc atttcctttc tcatagagaa
     9001 ccactcccct atctttcact attcttttaa tttcatcttg tctttcactt gatttaaaca
     9061 gctctggatt cttcctcaat cccccactat cttagttaat ggtagttgct tcagtactct
     9121 acattttttg ctaatcgagg ttaaaatcct tcaaatccct tgactttaag gtcttaaggt
     9181 tttcatactg gcgagtttgt taggaaatct gaatgtagtt ttgggcatag taacccctgt
     9241 tgaaaattta cgaggttaaa acgcccactc attcgtactg gcttagatgc cgaggtctcg
     9301 aattgagatt ggttgcattg acgacaaacg gttatagctt acaaaactac tataccgtcc
     9361 gtatatagct tattggcatt gtattgcgcc gtacggtgcg tttctcacgc ccaacaccgt
     9421 tccctttcgg gtgattcgct atataacccg catatccttg taataaagct tgtacaagaa
     9481 tagccgtgaa tcagtgtcat gatcgccgct tacgagtaac tgtttaactc cagtcgcaca
     9541 atcatgtctt attttgaacc cccaacactt tttgccgcta acgtcttggt tatcatcatt
     9601 attaggattt acatcccgta ggcttgtttt atgcaaggct tgtgtccttt acctcgctta
     9661 cgtcagacag ttgaatgcaa cgacaactga tatccgagtg tactctcctg cattggctac
     9721 actagaccat ctttttaaag tggtagcttc cactggaacg gaacacaaaa agggcgttct
     9781 ctatatctaa acgccctgta catttacaag acttctaggt atagaaaacg cccttttata
     9841 tccgttttat tatgtatatg acaatgctag taattactag ttgaaatatt cgtagagtaa
     9901 cggtacaata ggtgtatcta ataagccttg ttcgcgaaaa caaggcaaat aacttatttg
     9961 aggttggcgc ctcttataag tcgaagtcct gtatggctgt acaggattaa gtcattctcg
    10021 ctaaaggttg gtagccaata gcatatgaga gtggcttttt tcatttccgt tctattaagt
    10081 tactgctaat tttaccgcta tgtttctatt aaatcaacga aaatttgata aggttcacca
    10141 gaaacattaa tatttgcgac ataaaactca ctctccaact ttggaggatg gattattgaa
    10201 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    10261 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
    10321 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
    10381 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
    10441 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
    10501 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
    10561 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
    10621 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
    10681 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
    10741 agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat
    10801 tggagctcca ccgcggtggc ggccgctcta gaactagtgg atccatatgt attactagaa
    10861 aataacatag taaaacggac atcactccgt ttcaatggag gtgatgtccg tttttcatta
    10921 caacaaatta cttatctatt tgtaatgctg ctcttggaca attgatttga aaacgatatt
    10981 taaacctgag tttgcatgag actattcctc cataaggagt aattccacgc tgtccacgat
    11041 aaatgttcct tccgtttctc caatctcaat ccatacctta tcggtttctg ggaagtattc
    11101 taattctttt gtcacataac caactggtag tggcgtgtaa tccctatacc ctctgttaaa
    11161 ttcacaagga ttctctcttc gtccatctgt atacgatttt tcttcataga ctgacgcata
    11221 atcagctggt acggaaggag cttcgttata tcctcgatta cgagaagtgt acgcacctcc
    11281 gtattcttct tgatttacag tataatcatt acacgttacc gtgttatttg gatagatttc
    11341 ctcttctacg cagttgctaa acttcagttc gtctgtattg ttctcgatct catgaatggt
    11401 tacgcaacct tctccatatc cctccttgta cgctgtgaca cgaaggatat agccacgacc
    11461 cggacagaca cgaacttctt gtgacacttc tgcttcccat tccggaacaa caaggaccga
    11521 a
//
