LOCUS       NZ_CP116322             8513 bp    DNA     circular CON 01-MAR-2024
DEFINITION  Bacillus thuringiensis strain Lip plasmid pLip8513, complete
            sequence.
ACCESSION   NZ_CP116322
VERSION     NZ_CP116322.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32746259
            Assembly: GCF_028356555.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 8513)
  AUTHORS   Fayad,N., Barssoum,R., Marsaud,N., Nasseredine,R., Teste,M.A.,
            Pailler,V., Gautier,V., Belmonte,E., Aceves,C., Cescut,J.,
            Fillaudeau,L. and Kallassy Awad,M.
  TITLE     Complete genome sequences of two Bacillus thuringiensis serovar
            kurstaki strains isolated from Lebanon and Tunisia, highly toxic
            against lepidopteran larvae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8513)
  AUTHORS   Fayad,N., Barssoum,R., Marsaud,N., Nasseredine,R., Teste,M.A.,
            Pailler,V., Gautier,V., Belmonte,E., Aceves,C., Cescut,J.,
            Fillaudeau,L. and Kallassy Awad,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2023) Faculty of Science, Saint Joseph University
            of Beirut, Mar Roukoz, Mansourieh, Beirut 1514, Lebanon
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/01/2024 03:30:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,540
            CDSs (total)                      :: 6,405
            Genes (coding)                    :: 6,082
            CDSs (with protein)               :: 6,082
            Genes (RNA)                       :: 135
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 323
            CDSs (without protein)            :: 323
            Pseudo Genes (ambiguous residues) :: 0 of 323
            Pseudo Genes (frameshifted)       :: 113 of 323
            Pseudo Genes (incomplete)         :: 238 of 323
            Pseudo Genes (internal stop)      :: 74 of 323
            Pseudo Genes (multiple problems)  :: 78 of 323
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116322.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8513
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="Lip"
                     /isolation_source="soil sample"
                     /db_xref="taxon:1428"
                     /plasmid="pLip8513"
                     /geo_loc_name="Lebanon"
                     /collection_date="2013-01-01"
     gene            join(7314..8513,1..6)
                     /gene="mobV"
                     /locus_tag="PJW02_RS31695"
                     /old_locus_tag="PJW02_31695"
     CDS             join(7314..8513,1..6)
                     /gene="mobV"
                     /locus_tag="PJW02_RS31695"
                     /old_locus_tag="PJW02_31695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033080.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000033305.1"
                     /translation="MSKFVICHMQKFKMRDVKGLQIHNQREKESHSNSDIIQARTEQNY
                     DLIHDKEKVDYKKLIQNRIDNGVVSNRAIRKDAVVCCSFMISASPDYMNSLSQVEQRRF
                     FEESLRFLKGRYGEENFVYASVHVDEKTPHMHVGMVPVNEKQKLSAYSFFKNKSEFHDL
                     QDKIYEHVKEKGFDIERGVSSDRKHLSTQRFKAVTLQQEIEELEQEKKGIDSRLHDLKM
                     SLDQAKSVDEIPVKEKGGFIRSKTVEITSEDFESIKVLARSSEALREDVRRLKNESGVI
                     TREKDGLYREKCILEGQITELKRENRGLKEENDFFKKTLERVKELYKEKLPELAGVIGY
                     VKGSILDRMNCKFLKRYFTSDDEVRGAQKFLNHKQEHEEQQKRLKQVRSSQQKNRDQGL
                     ER"
                     /name="mobV"
     gene            476..1522
                     /locus_tag="PJW02_RS31700"
                     /old_locus_tag="PJW02_31700"
     CDS             476..1522
                     /locus_tag="PJW02_RS31700"
                     /old_locus_tag="PJW02_31700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085408.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001085408.1"
                     /translation="MAKKNQDEITGKLQPKKKQNIKIYEFIKKKMSESGKELFKSCSIF
                     NEFLATKDKKKKKRVKGNDCKNRFCPICAWRKAGKDAVKIATMMEAIKIEEKKEFLFLT
                     LTTPNIKADMVKSEIDRFNKAFKKLFDRRNIQRSIKGYIRKLEMTYDKERFITEEMYNN
                     KKRKAYYDSRGLKVDDHNPNYDTYNPHFHVLLCVDKNYFKRKELYIKQEEWLEMWREVT
                     DMPEITQVHIQKVELIREGNAVAEVAKYSAKDYEMSVSQEVFDVFYLALKGRQLIVYGG
                     LLKDYAKKYEDGELDKYKSTDKNEYYYRLIAMWNKDLMKFEQEYQELSESEKQEYNGHL
                     INEVDVEA"
                     /name="protein rep"
     gene            1526..1723
                     /locus_tag="PJW02_RS31705"
                     /old_locus_tag="PJW02_31705"
     CDS             1526..1723
                     /locus_tag="PJW02_RS31705"
                     /old_locus_tag="PJW02_31705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000861196.1"
                     /translation="MKVAGDLYYYCLGCKKFHEYEKIDHKGVNRKLCFYCFKIQSKKTK
                     IIGDAEGRMQICETCHKELF"
                     /name="hypothetical protein"
     gene            complement(2592..2855)
                     /locus_tag="PJW02_RS31710"
                     /old_locus_tag="PJW02_31710"
     CDS             complement(2592..2855)
                     /locus_tag="PJW02_RS31710"
                     /old_locus_tag="PJW02_31710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000874967.1"
                     /translation="MAFLTNYKANGKRYFYVEKYVGKKPYTCKQSERIYSIGNERITLE
                     RLTLWILDNSFIPNELIKIGISINDIENWREKVENTIKRYSL"
                     /name="hypothetical protein"
     gene            3129..3356
                     /locus_tag="PJW02_RS31715"
                     /old_locus_tag="PJW02_31715"
     CDS             3129..3356
                     /locus_tag="PJW02_RS31715"
                     /old_locus_tag="PJW02_31715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869869.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000869869.1"
                     /translation="MKVVSNIRMIMAKKNIDNISDLVRITGVSRNSINKLWHNENVSSL
                     KLDTLITICEKLDVELLDLIEYIRDDSETK"
                     /name="helix-turn-helix transcriptional regulator"
     gene            3828..4907
                     /locus_tag="PJW02_RS31720"
                     /old_locus_tag="PJW02_31720"
     CDS             3828..4907
                     /locus_tag="PJW02_RS31720"
                     /old_locus_tag="PJW02_31720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RapH N-terminal domain-containing protein"
                     /protein_id="WP_001102658.1"
                     /translation="MNVQLGNEQITKLLNDWYQEIRAQHIIKAKQLKQKIEKEINNIEE
                     DQNLLLYYSLIDLRYKILTNDYADREQSLEKIEQLKEHTNNFLDYYYHFFKGMHAMKTG
                     NYSETQKYYDIAEKLLEEIPDEVEKAEFNYAVATFYYHTHQALLATQYANKAKSFFSGK
                     LGYEIKTGACENTLGMACITLREFSVAEEYLLSAINKFEKHSENKLALIVRYNLGLLYA
                     DQNLSELAIRYLLESFENQEDDYKTMFLLAQEYYKLKQTNTVNTYIEKGLNVCNQEYKI
                     HFLFLQALNNKLSTEQLEKVMLDAIPYFEKQNLWKYIQDYTEELAIRFYEEKNKDKSNE
                     YFYKSYKAKRNLLERESLK"
                     /name="RapH N-terminal domain-containing protein"
     gene            4904..5050
                     /locus_tag="PJW02_RS31725"
                     /old_locus_tag="PJW02_31725"
     CDS             4904..5050
                     /locus_tag="PJW02_RS31725"
                     /old_locus_tag="PJW02_31725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000976019.1"
                     /translation="MMKKFSLILIGVACTTGIFFSQFNNSIQTHDAKEKNDIIQQYAHG
                     KDI"
                     /name="hypothetical protein"
     gene            5170..5751
                     /locus_tag="PJW02_RS31730"
                     /old_locus_tag="PJW02_31730"
     CDS             5170..5751
                     /locus_tag="PJW02_RS31730"
                     /old_locus_tag="PJW02_31730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000723966.1"
                     /translation="MKKISIERSNWRKTYKKMIPVLLVGGILAGNVAFVTNNVFAATTN
                     NNNIIQTKQSLVSGQDEKSINELAEAFQFMHEEAAIYNNQGEIVDFDFNKIEAKYGNDV
                     GLDALKQQIKKDKSDIAGLSFTSCMKDALYDFFGVNAVQAAFNGGLAYYLEKKLWKEAA
                     KIGVKFFIGSSVAGLAATLAYYGGKCAIYG"
                     /name="hypothetical protein"
     gene            5744..5941
                     /locus_tag="PJW02_RS31735"
                     /old_locus_tag="PJW02_31735"
     CDS             5744..5941
                     /locus_tag="PJW02_RS31735"
                     /old_locus_tag="PJW02_31735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000361625.1"
                     /translation="MDKSSILKQMYFILFPVTLFLSLNIYQWIVKSDYSFTGYIIQNLI
                     IVALALVAAIIAYLITKKNI"
                     /name="hypothetical protein"
     gene            6793..6963
                     /locus_tag="PJW02_RS31740"
                     /old_locus_tag="PJW02_31740"
     CDS             6793..6963
                     /locus_tag="PJW02_RS31740"
                     /old_locus_tag="PJW02_31740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001218510.1"
                     /translation="MALSLLGLIVFKKSWLVAEKNREDWRGCIAWQRDMLCSRGEYKKR
                     LFNTWMSTVKS"
                     /name="hypothetical protein"
     Typing          452..1504
                     /name="rep_cluster_617"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.934"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          7315..8513
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 agatagtgtg gttctgtttg agttttttgt tttttagatt tatagcgtta gcggaatttg
       61 ttttttataa cttgcttaag atgagtatgt ttctcagatg ttttatccgt taaaaaaaat
      121 ggttttgaac gaaaaaaaat gcccctgtcc acggagtgga tttaggggca tttttttatg
      181 atttttcgtg ttcgaaaaat cgagtttttt ctttctttaa tacataggta actatttcgg
      241 caacgaaatt gatgcctatg tattctttta gcccttatgt atcaagggtt tgtaaaaaaa
      301 aagacttgct ttttcaggcg gatagattta tcatttttag tgtcgaatta aaaatcataa
      361 ataccacgag aggagcaagt ccaaaaaaag gattcgtaca ccctttttta tatcgaatat
      421 gaaattcaga aattagaaat tctgatgttt ttcagatagg actaatatat caaatatggc
      481 aaagaaaaat caagatgaaa tcactggtaa attacaacct aaaaagaaac agaatatcaa
      541 gatttacgaa tttataaaga agaaaatgag tgagagcggg aaagaattgt ttaaatcgtg
      601 cagtatcttt aatgaatttt tggctacaaa ggataaaaag aagaagaaac gagtaaaggg
      661 gaatgattgt aaaaatcgat tttgtccgat ttgtgcttgg aggaaagcag ggaaggacgc
      721 ggtgaaaatc gcaacgatga tggaagcaat taagattgag gaaaagaaag agtttctttt
      781 tttaacgttg acgactccaa atattaaagc tgatatggtg aaaagtgaga ttgatagatt
      841 taataaagct tttaaaaagt tatttgatag acgtaatatt caacgttcta ttaagggtta
      901 tattagaaag ctagaaatga cgtatgataa agaacgattc atcactgaag aaatgtataa
      961 caacaaaaag agaaaagcat attatgattc tcgtggattg aaagtagacg atcataaccc
     1021 gaattatgat acatataatc ctcattttca tgtattgctt tgtgttgata aaaattattt
     1081 taaacgtaaa gagctttata ttaagcaaga agaatggtta gaaatgtggc gagaagtaac
     1141 agatatgcca gagattacgc aagtacatat tcaaaaagta gagttaatac gagaaggaaa
     1201 tgcagttgcg gaggttgcga agtattcagc gaaagactat gaaatgtcgg ttagtcaaga
     1261 agtgtttgac gtgttctatt tagcattaaa aggtcgtcaa ttaattgttt acggtggatt
     1321 attaaaagat tatgcgaaaa aatatgaaga tggcgaattg gacaagtata agagtacaga
     1381 caagaatgaa tattattata gattaatcgc aatgtggaat aaggatttaa tgaaatttga
     1441 acaagagtat caagagttat ctgagagtga gaagcaagag tataacggtc atttaattaa
     1501 tgaagtagat gtggaggcgt agtgtatgaa agtagcggga gatttatatt attattgtct
     1561 tggttgtaaa aagtttcatg aatatgaaaa aattgatcat aaaggtgtaa atagaaaatt
     1621 atgtttttat tgtttcaaga ttcaatctaa aaaaacgaaa attattggtg atgctgaagg
     1681 tagaatgcaa atttgtgaga cttgtcataa agaattgttt tagttggttg atttgtgaaa
     1741 aaagcaaagt caaatgttag aaatttttaa taagggaact gactgttata tcaagtcagt
     1801 tcccttattt tattgagtct atttttgagt gcagcaagta gcattcaaaa gtttttttat
     1861 gatttaacaa aaaggagttt ttcttaaatt taaatttcgt gaatttgtaa atttattttt
     1921 aaagtttatt tcgagatata ttttgttcta ctaattttgt tttactaacg tacgaggtga
     1981 aaacttaaag tgtgcgcatg aattttttta gtaatatata ttacgataaa ataccgcacg
     2041 ttttttgatg atttttttga aaatatagtt ttgagaaatt ctaaattttc ggggctgatt
     2101 ttggattttt tatgtgggat ttgattttgg aatttgttaa attggaaatc tcgatttttc
     2161 gattttcgga atttttgttt tctcttttct ttgttattct gtatttcgaa attaaggagt
     2221 ttatataata ccttcatgac gaactgaaaa cgaaaaaact ttgctcgcat atttaattca
     2281 tagatatttc atatcgttaa aaagttgcac cgtttcaaac gagttgtgtg ataaaaagat
     2341 gtttagatta tgtttgaaat tgttgtttct gtgaattttt tatttcgcga tttctgtata
     2401 tttttaatcc tttttactct taaaatcttt ttgcttttgt gcgcaaatga aaaaatcaaa
     2461 aagtcacgcg taacttttat taaatatata aatatattat taaaatacct aaccctttta
     2521 tgatgaaaaa atataaatag ttaaagaaaa aagactgata aaaaatatca gtcttttttc
     2581 tttagtttta tttataaaga gtatctcttt atggtgtttt ctactttttc tctccagttc
     2641 tctatatcgt taattgatat tccgatcttt attaattcgt ttggaatgaa actgttatct
     2701 aaaatccata aagtgagcct ttctaatgtg attctttcgt ttccaattga ataaatacgt
     2761 tcactttgtt tgcaagtata tggtttttta ccaacatatt tttctacata aaagtatctt
     2821 tttccgtttg ctttgtaatt tgttaggaat gccaaaatga taatctcctc ttagtttttt
     2881 tagagttaaa aatacatttt aaaagattaa attttaagtt atatatttaa ttttaactct
     2941 aaggggttaa aatttcaatt aaaacaaata aaattaaata ataaaacaat atattttatt
     3001 tttaaggtta atattaaatg tgaatatata aaaaataacc ttaatattat ttaatttatg
     3061 ttttatttgt ataaaagtat ccttgtaagt aaaatggtat gtacattgaa tgaaaaggag
     3121 tatgaaaaat gaaggtagta agtaatattc gtatgattat ggcgaaaaag aatatagata
     3181 atatttcgga tttggtgaga ataacgggag taagtcgtaa ttctattaat aagttgtggc
     3241 acaatgagaa tgtgtcttct ttaaaattag atacattgat aacgatctgt gagaagttgg
     3301 atgttgagtt attggattta attgaatata ttcgagatga tagtgagaca aaataattta
     3361 ggggctattt tagctttaga tagcttgtgt atgatgaaag catatttttg ctaatcgaca
     3421 atgagttata ttcacatatg ttttttttgt agggctatat gtgtatttaa agttttgatt
     3481 tctatttgtt attgtatgaa tatggtttga atgtggtaaa tgaaattata gtcttgctag
     3541 gtgcgtcaac accttggaag atgagcggta ctggcatact tcgtgatttc attgatagta
     3601 gtgcagtaaa gtgatggaat tgatgagtat tgaaaaagag ttcctgtttg ggggctcttt
     3661 ttttatttgt tactaagtat ttttaaatag tgaaggtgct acactattta aaaatacaga
     3721 atattaaaaa ctttatatat caactgttga aaagttagtc ttatacaata gcatgataac
     3781 atgatagaaa ataaaatata gataattttg gaaaaggaga agattctatg aatgttcaac
     3841 taggaaacga acaaattaca aaattattaa atgattggta tcaagaaatt cgagcgcaac
     3901 acattattaa agcaaaacaa ttaaaacaaa agattgaaaa agaaattaac aacatagaag
     3961 aagaccaaaa tttactttta tattattcac ttattgattt gagatacaag attcttacaa
     4021 acgactatgc agatagagaa caatctttag aaaaaatcga acaattaaaa gaacacacaa
     4081 ataatttttt agattactat tatcatttct ttaaaggcat gcatgcaatg aaaacaggaa
     4141 attatagtga aactcaaaaa tattatgata tagcagaaaa attactagaa gaaattcctg
     4201 atgaagtaga aaaagctgaa tttaattatg ctgttgcaac tttctattat catactcatc
     4261 aagccttatt agcaactcaa tatgctaaca aagccaaaag tttttttagt gggaaactag
     4321 gatacgagat taaaaccgga gcatgtgaaa acacacttgg tatggcttgt attacattaa
     4381 gggaattttc tgttgcagaa gaatacttgc tttccgcaat taataaattt gaaaaacaca
     4441 gtgaaaacaa attagcttta atcgtacgtt ataacttagg tcttttatat gcagatcaaa
     4501 atctttctga acttgcaatc cgttatcttc ttgaatcctt tgaaaatcaa gaagacgatt
     4561 acaaaacaat gtttttatta gcacaagaat attacaagtt aaaacaaaca aatacagtaa
     4621 acacatatat tgaaaaaggg ctaaatgttt gtaaccaaga atataaaatt catttcttat
     4681 ttttacaagc attaaacaat aaattatcta ctgaacaatt agaaaaagtc atgttagacg
     4741 cgattccata ttttgaaaaa caaaatctat ggaaatacat acaagattat actgaagaat
     4801 tggctataag attttatgaa gaaaagaata aggataaatc taatgaatat ttttataaaa
     4861 gttacaaagc taaaagaaac ttattagaaa gggagtcatt gaaatgatga aaaaatttag
     4921 tctgatacta ataggggtag catgtacaac aggtattttc ttttcccaat tcaataattc
     4981 cattcaaaca catgatgcta aagaaaaaaa tgatattatt caacaatatg cacatggaaa
     5041 agatatttaa aagcaaattc caacaattag caatcgacct aaatattagg attattaaca
     5101 aaatattgaa ttatatgtaa attaagttta ttatctattt tgaataataa acttaagggg
     5161 atgaaattta tgaaaaaaat tagtattgag agaagtaatt ggagaaaaac atataaaaaa
     5221 atgattccag tattgttagt cggaggaata ttggcaggaa atgtagcatt tgtgacaaat
     5281 aatgtttttg cggcaactac aaataacaac aatataatac aaacgaaaca gtctctcgtt
     5341 tctggtcaag atgaaaagag cattaatgaa ttagcagaag cgtttcaatt tatgcatgaa
     5401 gaagccgcta tatataataa tcaaggtgaa attgttgatt ttgattttaa taaaatagaa
     5461 gcgaaatatg ggaatgatgt tggtttagat gcacttaaac aacaaattaa aaaagataaa
     5521 agtgatattg ctggtttatc ttttacaagt tgcatgaaag atgctttata tgactttttt
     5581 ggagtaaatg ctgtacaagc cgcatttaat ggtggattgg catattatct tgagaaaaaa
     5641 ttatggaaag aagcggctaa aataggcgtt aaattcttta taggaagtag cgtagcaggt
     5701 ttagcggcta ctttagctta ttatggaggt aagtgtgcaa tttatggata aaagttcaat
     5761 tttaaaacaa atgtatttta ttttatttcc ggtaacactt tttctatctc taaatattta
     5821 tcaatggatt gttaaaagtg attactcatt tacaggatat ataatccaaa atttaattat
     5881 tgtagcatta gcgttagtag cagctatcat agcttattta attactaaaa aaaatattta
     5941 acacataatg ttagtctaaa taacagaaaa ttctaaccct tattcttatt ttgtattata
     6001 ctggttatgt acaggtaaaa tggtttacaa gttaattttt cctcgtttca aaaaaagtcc
     6061 attcttttca aaagaatgga cttttttctt gttatatttg ctgtaactat taaaagggca
     6121 tcaaaacgat ctacaaaagc ctcaagacga cccacaaacc atctttaaac ggtctcaaaa
     6181 cgaactacaa cccgaaaaaa cgcatcccag tttttttagg tttaatttta agcgcatacc
     6241 aagcgcatat aggcgcatac gggacacata caaagtgccg aaaaacgcat ataggtcgca
     6301 tacaaacggt agaaatacat ccctaaaaat agagtgatag aaacccaata ggcacagcct
     6361 atttcaccac tttttttgca aagtgggatt cggtttcctt atgctctttt tttatttttg
     6421 ctttttaagg gtcttataat cgtttttaag tattttagga tgttgggttt cttaagggaa
     6481 ctagaagttc ctagaatgga ttctgagcgt tctgaggtgt atttttctat ggattcgata
     6541 ttggaattgc tctaggaata gttgtcgttt tattctgtgg ctgttccgac agtggttctc
     6601 ataaagaata tgattgtatc aatagatttg atgatgtttg aaaaatagag aatagttgaa
     6661 gctcttcctg taaaacaaaa aaaattatgt ttgtagagga agtagtcatt tcactacaag
     6721 aaaattccca tagatcggtg gcgagttgaa aaatgttgaa ggttggcgag gacattttac
     6781 gaggacgcgg gaatggcttt gtcattgttg gggttaatag tttttaagaa aagttggtta
     6841 gtagctgaaa aaaataggga agattggcga ggatgtattg catggcagcg ggacatgtta
     6901 tgtagtcgtg gtgaatataa aaagagactt tttaatacat ggatgtcaac tgtaaaaagt
     6961 tagaggtagt tgtatgatgt ttgtccaacg gtaagcggaa cgaagtgagc attaaggagt
     7021 ggacgaaccc actgtgccca gaaccctctt ggaactccaa aacgttagcc gttttgggga
     7081 tgccaaccgg cgagggagtc cctcaaggat tgaggggttg gggagttcga ttgaaagggg
     7141 tcaaggggaa gagtttccct tgtgggtttg ggcaaagccc atgatttttt ttgccatgct
     7201 tgttatggct cggcggtagc cgaaatgtcc cgaagggcac acgtgtttac gcagtaaact
     7261 gtagtgtgct actgtttaca tggaactacc cccgaaaaaa aggagcgagg cccatgtcga
     7321 agttcgtgat ttgccatatg caaaaattta agatgagaga tgtgaaagga ttacaaatac
     7381 ataatcaacg tgaaaaagaa agtcattcca attcagatat tattcaagca cgaacggaac
     7441 agaattatga tctgattcat gacaaagaaa aagtggatta taagaaatta attcaaaatc
     7501 gaattgataa tggtgttgta tctaatcgtg caattcggaa agacgctgtt gtttgttgtt
     7561 cgttcatgat ttcggctagt ccggattata tgaattcttt atctcaagtg gaacagagac
     7621 gattctttga agagtctctt cgttttttga aggggagata tggagaagag aactttgtgt
     7681 atgcttccgt acatgttgat gaaaaaacac ctcatatgca tgtgggtatg gttccagtta
     7741 atgaaaaaca aaaactatca gcgtattcat tttttaaaaa caaatcagag tttcatgatt
     7801 tacaagataa aatctatgaa catgtgaaag aaaagggatt tgatatagag cgtggtgttt
     7861 ctagtgatag aaaacattta tcgacgcagc ggtttaaagc ggtgacttta caacaagaaa
     7921 tagaagaact agagcaagaa aagaagggaa tagatagtcg tcttcatgat ttaaaaatgt
     7981 ctttagatca agcaaaaagt gtggatgaga tcccagttaa agaaaaaggt gggtttatac
     8041 gctctaaaac ggtcgagatt acctcggaag acttcgaaag tataaaagta ttagctaggt
     8101 cgtcggaagc ccttagagaa gatgttagac gccttaaaaa cgaatcaggt gtaattacac
     8161 gtgaaaaaga tgggctgtat agagagaaat gtattttaga ggggcaaata acagaattga
     8221 aaagagagaa tcgaggattg aaggaagaga atgatttctt taagaaaacg ttggaacgag
     8281 tgaaagagtt gtataaagaa aagcttcctg aattggctgg ggtgattggt tacgttaagg
     8341 ggagtatatt ggacaggatg aattgcaaat ttttaaaacg atattttact agtgatgatg
     8401 aagtgagagg cgctcaaaag tttttgaatc ataaacaaga gcatgaagaa caacaaaaga
     8461 gattaaaaca agtgcgaagt agccaacaga aaaatcggga ccagggattg gag
//
