LOCUS       NZ_CP116321             7911 bp    DNA     circular CON 01-MAR-2024
DEFINITION  Bacillus thuringiensis strain Lip plasmid pLip7911, complete
            sequence.
ACCESSION   NZ_CP116321
VERSION     NZ_CP116321.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32746259
            Assembly: GCF_028356555.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 7911)
  AUTHORS   Fayad,N., Barssoum,R., Marsaud,N., Nasseredine,R., Teste,M.A.,
            Pailler,V., Gautier,V., Belmonte,E., Aceves,C., Cescut,J.,
            Fillaudeau,L. and Kallassy Awad,M.
  TITLE     Complete genome sequences of two Bacillus thuringiensis serovar
            kurstaki strains isolated from Lebanon and Tunisia, highly toxic
            against lepidopteran larvae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7911)
  AUTHORS   Fayad,N., Barssoum,R., Marsaud,N., Nasseredine,R., Teste,M.A.,
            Pailler,V., Gautier,V., Belmonte,E., Aceves,C., Cescut,J.,
            Fillaudeau,L. and Kallassy Awad,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2023) Faculty of Science, Saint Joseph University
            of Beirut, Mar Roukoz, Mansourieh, Beirut 1514, Lebanon
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/01/2024 03:30:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,540
            CDSs (total)                      :: 6,405
            Genes (coding)                    :: 6,082
            CDSs (with protein)               :: 6,082
            Genes (RNA)                       :: 135
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 323
            CDSs (without protein)            :: 323
            Pseudo Genes (ambiguous residues) :: 0 of 323
            Pseudo Genes (frameshifted)       :: 113 of 323
            Pseudo Genes (incomplete)         :: 238 of 323
            Pseudo Genes (internal stop)      :: 74 of 323
            Pseudo Genes (multiple problems)  :: 78 of 323
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116321.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7911
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="Lip"
                     /isolation_source="soil sample"
                     /db_xref="taxon:1428"
                     /plasmid="pLip7911"
                     /geo_loc_name="Lebanon"
                     /collection_date="2013-01-01"
     gene            complement(join(7759..7911,1..125))
                     /locus_tag="PJW02_RS31645"
                     /old_locus_tag="PJW02_31645"
                     /pseudo=""
     CDS             complement(join(7759..7911,1..125))
                     /locus_tag="PJW02_RS31645"
                     /old_locus_tag="PJW02_31645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332102.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            314..541
                     /locus_tag="PJW02_RS31650"
                     /old_locus_tag="PJW02_31650"
     CDS             314..541
                     /locus_tag="PJW02_RS31650"
                     /old_locus_tag="PJW02_31650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869766.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000780193.1"
                     /translation="MKLVNNIRMIMAQKNIDNIAELIRITGVSRNSINKLWHNESVSSL
                     RLDTLIAICEKLDVKLSDLIEYIPGDIESK"
                     /name="helix-turn-helix transcriptional regulator"
     gene            1648..2853
                     /gene="mobV"
                     /locus_tag="PJW02_RS31655"
                     /old_locus_tag="PJW02_31655"
     CDS             1648..2853
                     /gene="mobV"
                     /locus_tag="PJW02_RS31655"
                     /old_locus_tag="PJW02_31655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033080.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_040120001.1"
                     /translation="MSRFMVCHMQKFKMTDVKGLQIHNQREKESHSNPDIIQERTEQNY
                     DLIHGKENVDYKKLIQDRIQDGVVSDRAIRKDAVVCCSFMISASPDYMNSLSQAEQRRF
                     FEESVCFLKARYGEENFVYASVHVDEKTPHMHVGMVPVNEKQKLSAYSFFKNKSELHDL
                     QDKIYEHVKEKGFDIERGVSSDRKHLSTQRFKAVTLQQEIEKLEQEKKEIDSRLHDLKL
                     SLDKAKSVDEIPVKEKGGFIRSKTVEIALEDFESIKVLAKSSETLREENKHLKNEKVKD
                     EYEKDNLYKEQRFLERKVTDLKRENEGLKGENDFLKKTLERVKDLYKEKLPELAGMIGY
                     VKASILDKMNRKFLKRHFAGDDEVSGAQKFLNHKQEHEEQQKRLKQVRRSQQKNRDQGL
                     ER"
                     /name="mobV"
     gene            2885..3166
                     /locus_tag="PJW02_RS31660"
                     /old_locus_tag="PJW02_31660"
     CDS             2885..3166
                     /locus_tag="PJW02_RS31660"
                     /old_locus_tag="PJW02_31660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002026684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002026684.1"
                     /translation="MDYFHGYGVKFIGTVKELAKKNNLTIEETCRILELTMKIDDYDRK
                     DEQLAGIGELIKEMNEQIMGLGEGLSAISERLRDMSEGIALISMDDKE"
                     /name="hypothetical protein"
     gene            3431..3640
                     /locus_tag="PJW02_RS31665"
                     /old_locus_tag="PJW02_31665"
     CDS             3431..3640
                     /locus_tag="PJW02_RS31665"
                     /old_locus_tag="PJW02_31665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001174425.1"
                     /translation="MQKVIVVNGYWHNSNGKWKDTTEELNGLLAEGWKVVNIAPMGAYG
                     FGYDAMGNTANSDHGFASIVVIEK"
                     /name="hypothetical protein"
     gene            3903..4253
                     /locus_tag="PJW02_RS31670"
                     /old_locus_tag="PJW02_31670"
     CDS             3903..4253
                     /locus_tag="PJW02_RS31670"
                     /old_locus_tag="PJW02_31670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002026683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000562819.1"
                     /translation="MICDNCMNEVEKEFCQTCHYFGYVEVRYKCSNCSYEGKTIENLDD
                     KVNELFKKGRKLIIEMDTDFKPYLEILVKRGITNPKEVGILFGMYLESKKEDHEGYKTI
                     SKSGFEKFLTTI"
                     /name="hypothetical protein"
     gene            4390..4596
                     /locus_tag="PJW02_RS31675"
                     /old_locus_tag="PJW02_31675"
     CDS             4390..4596
                     /locus_tag="PJW02_RS31675"
                     /old_locus_tag="PJW02_31675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001029683.1"
                     /translation="MAKANYVTVGRKPKNGKTPKVQEAFRLPKETVDRINELVNSGRFS
                     KKIDVAEKAFEFYFAHLDKEERK"
                     /name="hypothetical protein"
     gene            4757..6139
                     /locus_tag="PJW02_RS31680"
                     /old_locus_tag="PJW02_31680"
     CDS             4757..6139
                     /locus_tag="PJW02_RS31680"
                     /old_locus_tag="PJW02_31680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000648.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_040120002.1"
                     /translation="MHNVLKSSLLDVTIDKLSIVADFKEGIKKEEFIRMVASSNMPYSL
                     QANRSRNFGYEEVLKSESVDCGYIELAGELKVASVDRPKLTFHRFRLQDQIEQERLTRK
                     NGEPSLSDVEYQGLFESLADVEELIAETDEDGRLTDERKLKRQEKQVELTIEKLKGEIV
                     ALKSEIDEQELATGFKNEAMEKIHEEHVQLLLNTLKNKKNLIEVKKDELLRNAEMFLQL
                     DENGFLPGNGKKKTDRLLKNVRFEFNPKHMAYNRVVDESIRLVLGLLENVEVTGIHIAC
                     DYPVKISNLKIKDLSSKSECIFLDRDKSLGTMYVGRRGSDNHLCIYDKKQENRENDTLD
                     QYPDVEHVTRFEARLRKKKAREWIESDYNPFDSIIVGDLVNLDDSEMKLGDRIILEAIM
                     NDYHGRYFGMMDRFQRNTWRKKLNQCVPKMFDVAGDYEKKKVLLTGQLAELLENGKNKV
                     GK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            6571..6777
                     /locus_tag="PJW02_RS31685"
                     /old_locus_tag="PJW02_31685"
     CDS             6571..6777
                     /locus_tag="PJW02_RS31685"
                     /old_locus_tag="PJW02_31685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000947848.1"
                     /translation="MLSEQVIYYWNDELQYYYLAEKKNGSYKFVQPLKKLELDDISDPL
                     EREQMKKMLEIKKRMSETKGNWF"
                     /name="hypothetical protein"
     gene            complement(7482..7745)
                     /locus_tag="PJW02_RS31690"
                     /old_locus_tag="PJW02_31690"
     CDS             complement(7482..7745)
                     /locus_tag="PJW02_RS31690"
                     /old_locus_tag="PJW02_31690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000874968.1"
                     /translation="MAFLTNYKANGKRYFYVEKYVGKKPYTCKQSERIYSIGNERITLE
                     RLTLWILDNSFIPSELIKIGISIDDIENWREKVENTIKRYSL"
                     /name="hypothetical protein"
     Typing          4758..6139
                     /name="rep_cluster_268"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1649..2850
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.524"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgattgccga ttgagtaaat atttatatat ttatagtgtt ttgagtaata tggttgtgtt
       61 ccaacgtatt gagatacata aaagtaaatg tttcctttta ctttttttgc tgttagatat
      121 gccaaatcgt tcttcctttc atgtttgtta ttcttttgtt tttgtctgtg gttatatatt
      181 aaccttataa agttaaaata tcaatacata taaataaata taaaatttaa aaggtttatt
      241 tttatttata aattgcataa aattatcttt ataagtaaaa tagaatgtat gttgaatata
      301 aggagtaaga ggaatgaagt tagtaaataa cattcgtatg attatggccc aaaaaaatat
      361 agataatatt gcggagttaa taaggataac tggtgtgagt cgtaattcta ttaataagct
      421 ttggcataat gaaagtgtgt cgtctttaag attagatact ttaatagcga tctgtgagaa
      481 gttagatgtt aaattatcgg acttaattga atatattcca ggtgacattg agtcaaaata
      541 atttagggga ttttatgtac ttggatagtt tttgtatgct aaaggtatag ttggctaatg
      601 gtatttggtg gtgctataag gcgcacttca acttttttgt taacgcttga gtgtggtttg
      661 gattagggat atgaaattgt agacttgcta ggtgcgtcaa caccttggaa gatgagaggt
      721 actggcatac ttcgtgattt tgttgaactt tatttataat catagtagtg cggttaaatg
      781 aaatggtggt attgattagt attgaagaaa gagtcctatt ttagggctct tttttatttt
      841 gccttttaaa gggcttaaat cgtttttaaa acattgtgag gctcttagga tttcttaaga
      901 actagaagtt cctagaatgc attctgaacg ttctgaggtg tatttctcta tggattctgt
      961 cttggaatgg gtctaggaat agctgtagga atagctgtag ttttattcta tggttgttcc
     1021 ggtagtggtt ctcatgaaga atatgattgt atcaatagat ttgatgatgt ttgaaaagta
     1081 gagaatagtt gaagctcttc ctgtaaaaca aaaaaataat tatgtttgta gaggaagtag
     1141 tcatttcact ccaagaaaat ccctgtagat cggtggggcg gtgaaaaaaa atagggaaga
     1201 ttagcgaggg tattttgcag ggcagcggaa gacgttatgt tgtcgtgatg aatataaaaa
     1261 gagacttttt aatacatgga tgttagctga aaaacgtttg aggtagttgt atgatgtttg
     1321 tccaacggta agcggaacga agtgagcatt aaggagtgga cgaacccact gtgcccagaa
     1381 ccctcttgga actccaaaac gttagccgtt ttggggatgc ctaccggcga gggagtccct
     1441 caaggattga ggggttgggg agttcgattg aaaggggtca aggggaagag tttcccttgt
     1501 gggtttgggc aaagcccatg attcttttgc catgcttgtc atggctcggc ggtcgccgaa
     1561 atgtcccgta gggcacacgt gtttacgcag taaactgtag tgtgctacag tttacatgga
     1621 actaccaccg aaaaaggagc gaagcccatg tcaagattca tggtttgtca tatgcaaaaa
     1681 ttcaagatga cagatgtgaa aggattacaa atacataatc aacgtgaaaa agaaagtcat
     1741 tcaaatccag atattattca agaacgaacg gaacagaatt atgatttgat tcatggtaaa
     1801 gagaatgtgg attataaaaa actaattcaa gatagaattc aagacggggt tgtatctgat
     1861 agagccatta ggaaagatgc tgttgtttgt tgttcgttta tgatttcggc tagtcctgat
     1921 tatatgaatt ctttatctca agcggaacag agacgattct ttgaagagtc tgtttgtttt
     1981 ttaaaggcaa ggtatggaga agagaacttt gtatatgctt cagtacatgt tgatgaaaag
     2041 acacctcata tgcatgtggg catggttcca gttaatgaaa aacaaaaact atcagcgtat
     2101 tcatttttta aaaacaaatc agagcttcat gatctacaag ataaaatcta tgaacatgtg
     2161 aaagaaaagg gatttgatat agagcgtggt gtttctagcg acaggaaaca tttatcgacg
     2221 cagcggttta aagcagtgac tttacaacaa gaaatagaga agttagaaca ggaaaagaaa
     2281 gagatagata gtcgtcttca tgatttgaaa ttatctttag ataaagctaa aagtgtagat
     2341 gagattcctg ttaaagagaa aggtgggttt atacgctcta aaacggtcga gattgcatta
     2401 gaagactttg aaagtataaa agtattagct aagtcttccg aaacgcttag agaggaaaat
     2461 aaacacctta aaaacgaaaa ggttaaggat gaatatgaaa aagataattt atataaagaa
     2521 caacgttttt tagagaggaa agtaactgat ctaaaaagag agaatgaagg attaaagggc
     2581 gaaaatgatt ttcttaagaa aacgttggag cgagtgaaag atttgtataa ggagaagctc
     2641 cctgaacttg caggaatgat tggttatgtt aaagcgagta tactggataa gatgaatcgc
     2701 aagtttttaa aacgacattt tgctggcgat gacgaagtaa gtggtgctca aaagttcttg
     2761 aatcacaaac aagaacatga agaacaacaa aagagattaa aacaagtgcg aagaagccaa
     2821 cagaaaaatc gggaccaggg attggagaga tagtgtgggg acattattta atcagcaacc
     2881 aagaatggat tattttcatg gttatggagt taagttcata ggtactgtga aggaattggc
     2941 taagaaaaat aatttaacga ttgaagagac gtgtagaatt ttagaattga ctatgaaaat
     3001 tgatgattat gatcgtaagg atgagcagtt agcaggtatt ggagaactta ttaaggaaat
     3061 gaatgaacaa attatggggc ttggtgaagg tttgagtgct ataagtgaac gattgcgtga
     3121 tatgagtgaa ggtattgcgt taatatctat ggatgataaa gaataatggt atgttaggag
     3181 gctctgtgtg agtctctttt tttctttaga cgtgtagtgt tagcggaact tgtttggcat
     3241 aatttaacga ggtttgtaga gctttgcata atgtcctcgc aaattgttga tgtgaatata
     3301 tggggatatt atataaggct cttgggaatg gctcttgttt tttaaaatat ttatttagat
     3361 atatatatta aaattgtatt tgatatatag aaatttatat attgataatc aaaacgaggg
     3421 agaggtatat atgcaaaagg taattgtagt taatggttat tggcataata gtaatggtaa
     3481 atggaaggac actacagaag aattgaatgg attgttagca gaaggatgga aagttgttaa
     3541 tatagcacct atgggggctt atgggtttgg ttatgacgca atggggaata cggctaattc
     3601 tgatcatggt tttgcctcga tagttgttat tgagaagtaa tagaaaagac gtgggatttc
     3661 accacgtctt tttttatttt gaaagtaaga ttaaggaata acccccccta tgtaatacgg
     3721 gttgtgtgac ctcaaaaact tagggggtaa ttctattgaa aatacaggga tattaaggct
     3781 ttaaggtgtg ttttcttttc ttttaaaaat ggaattttaa aagaatgaac aaaaaataca
     3841 gtaaaatata caaaaggtag acggtaaaaa aatagatatt atgtttctgt agggggagaa
     3901 aaatgatttg tgataattgc atgaatgaag tagaaaagga attttgtcaa acatgtcatt
     3961 attttggtta tgttgaggtt cgatataaat gttcgaattg tagttatgaa ggcaaaacga
     4021 ttgagaactt agatgacaaa gtaaatgaac tgtttaagaa ggggagaaaa cttattattg
     4081 aaatggatac agattttaag ccttacttag aaattctagt gaaacgtggt atcacgaatc
     4141 ctaaagaggt tggaattttg tttggtatgt atttagaaag taaaaaggaa gatcatgagg
     4201 gatataaaac tatttctaag tctggatttg aaaaatttct aacaacgatt tgagttttag
     4261 taggtgagca atttttgata atttgtagac tttgaagttt atattgaaat gaattttaaa
     4321 attaaacttg ttaaaatggt atgcaaaatg atatcatgat attgttggtt ttatcgaggg
     4381 ggtgaaaaga tggctaaggc gaattatgtg actgttggaa ggaagcctaa aaacggaaaa
     4441 actccaaaag tacaagaagc tttcagattg ccgaaagaaa cggttgatag aattaatgaa
     4501 ttagtgaata gtgggcgttt ttctaaaaag atagatgttg cagaaaaagc gtttgaattt
     4561 tattttgctc atttagacaa agaagaaaga aagtgatttg tttttagtgt tttttggtgg
     4621 ggaagaaata gtccgatgaa aaaaagttac ctgttattct aataagcagg taaaggttga
     4681 gaatttctca cacttcttaa aaaaacatct cggacaagcg cacaaaaata tctgttttgg
     4741 gggttaagga tttgttatgc ataacgtact gaaatcaagt ttgttagatg taacgattga
     4801 taaattaagt attgttgcag attttaagga aggcattaaa aaagaagagt ttatacggat
     4861 ggtggcgagt tcaaatatgc cgtattcgtt acaggcgaat cgttcgagga attttggtta
     4921 tgaagaagtt ttaaaaagtg aatctgttga ttgtggttac attgagttgg caggagaatt
     4981 gaaagttgca tctgttgatc gtccgaagtt aacttttcat agatttagat tgcaagatca
     5041 aattgagcaa gaaaggttaa cgcggaaaaa tggtgaacca tcactttctg atgtggaata
     5101 tcaaggtttg tttgaaagtt tagctgatgt agaagaattg atagctgaaa ctgatgaaga
     5161 tggtcgtttg actgatgaga gaaaattaaa acggcaagaa aaacaagtgg aattgactat
     5221 tgagaagttg aaaggcgaaa tagttgcatt gaaatctgaa atagatgagc aagaacttgc
     5281 aacgggtttt aaaaatgaag ctatggaaaa aattcatgag gaacatgttc agttattact
     5341 taatacttta aagaacaaaa agaacttgat tgaagttaaa aaggatgagt tgttaaggaa
     5401 tgctgaaatg tttttacagt tagatgaaaa tggttttctt ccgggtaatg gaaaaaagaa
     5461 aactgatcgt ttgttgaaaa atgttaggtt tgagtttaac cctaagcata tggcatataa
     5521 tcgtgtggtt gatgagtcga tacgattagt tttggggttg ttagaaaatg ttgaagttac
     5581 aggtatacat atagcttgtg attatcccgt taagatttca aatttgaaaa ttaaagattt
     5641 atcaagtaaa tcagagtgta tttttctaga tagggataaa tctttaggaa cgatgtatgt
     5701 tggaagacgc gggtcagata atcatctttg tatttatgat aagaagcaag agaatagaga
     5761 gaatgatact ttggatcagt atccagatgt ggaacatgta actcgttttg aagctcgttt
     5821 acgtaagaaa aaggcgagag aatggataga gtcagattat aatccgtttg attcgataat
     5881 cgttggggat ttagtgaatt tagatgatag tgaaatgaaa cttggtgata ggattatttt
     5941 agaagctatt atgaatgatt atcatgggcg ttattttggg atgatggata gatttcaaag
     6001 aaatacatgg cgtaagaaat tgaatcaatg tgttccaaaa atgtttgatg ttgctgggga
     6061 ttatgaaaag aaaaaagttc tactaactgg acaattagca gaacttctag aaaatggtaa
     6121 aaataaggtt ggaaaataaa aaaaatggtt aatcctttcg agtgttggcg cacttggaag
     6181 gattttttta tgatttagaa cttaatttta tttttaagaa taactacgac tattattgca
     6241 tgtataccgt atattattca ttttaatgac ttttatattg attgtcatca aagaaaaata
     6301 ccccaaaaag ggtgcggttt tttatatata taaaacatat aaccctaccg ttcataaatt
     6361 atatatattt ttatgaacgt ttgcaaatga ttttagggac tttttgagac acttgttttt
     6421 ggctttatga tgcgtttatc agttatttgg gactactttt tgatacggtt acttttggaa
     6481 ctgtctcttt tgtattgcgt taattttagg gctttattga tgctatgatg gtgtaatggg
     6541 ctaatattaa ggtgattact gggggctttt atgttgagtg aacaagtcat atattattgg
     6601 aatgatgaat tacagtatta ttatttagct gaaaaaaaga atggcagcta taaatttgta
     6661 caaccgttaa aaaaattaga attagatgat attagtgatc cgttagaaag agaacaaatg
     6721 aaaaaaatgc tagagataaa aaaacggatg tcagaaacaa aaggtaattg gttttaaaaa
     6781 aaagaaagac aaaatgtctt tctttttttt aaaactttaa tttcgtgaat ttgtgaattc
     6841 ttttttaaat ttataagcta tatagtttat atcctaaaac ttcccgttac taccgtacag
     6901 gatggaaaac taaagtgtgc gcatgaattt ttttagttat atatataacg ataaaatacc
     6961 gtacgttttt tgaaggttta ttttttgggg gagattttga tgtggaattt gattttgaac
     7021 tttgttgaat ttgaaatctc gatttttcga tttttggaat ttttgttttc tgttttcttt
     7081 gtaattccgt attccgaaat tacggagttt atatagtatc tctatgatga actgaaaacg
     7141 aaaaaaactt tgctcgcatt ttcatttcat agatatatat atcattaaaa agtcgcaccg
     7201 ttttaatcga gttgtgtgat gaaaaaaaga tgtttagatt atgtttgaat ttgttgtttt
     7261 gtgaattttt tatttcgtga tttctgtata tttttaatcc tttttactct taaagtctgt
     7321 ttgtttttgt tcgcaaatga aaaattcaca aagtcacgca taacttttat taaatatata
     7381 aatatattat taaaatatct aacccttttg tgatgaaaaa aatataaata gttaaaggaa
     7441 aaaagactga taaaaatatc agtctttttt cttgttttta gttatagaga gtatcttttt
     7501 atggtatttt ctactttttc tctccagttt tctatatcgt caattgatat tccgatcttt
     7561 attaattcgc ttggaatgaa actgttatct aaaatccata aagtgagtct ttctaatgtg
     7621 attctttcgt ttccaatcga ataaatacgt tcactttgtt tgcaggtata tggtttttta
     7681 ccaacatatt tttctacata aaagtatctt tttccatttg ctttgtaatt tgttagaaat
     7741 gccatagtaa tgctcctttt aggtattttt atggttagtt aaagagtagt tttgtaatgt
     7801 tttttcaact ttttcatacc aatatttcac atcgtttatg gatactccga tttctattag
     7861 ttcttttggt atgcggttgt tatctaataa ccacatagcg attctctcta a
//
