LOCUS       NZ_CP116320             7635 bp    DNA     circular CON 01-MAR-2024
DEFINITION  Bacillus thuringiensis strain Lip plasmid pLip7635, complete
            sequence.
ACCESSION   NZ_CP116320
VERSION     NZ_CP116320.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32746259
            Assembly: GCF_028356555.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 7635)
  AUTHORS   Fayad,N., Barssoum,R., Marsaud,N., Nasseredine,R., Teste,M.A.,
            Pailler,V., Gautier,V., Belmonte,E., Aceves,C., Cescut,J.,
            Fillaudeau,L. and Kallassy Awad,M.
  TITLE     Complete genome sequences of two Bacillus thuringiensis serovar
            kurstaki strains isolated from Lebanon and Tunisia, highly toxic
            against lepidopteran larvae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7635)
  AUTHORS   Fayad,N., Barssoum,R., Marsaud,N., Nasseredine,R., Teste,M.A.,
            Pailler,V., Gautier,V., Belmonte,E., Aceves,C., Cescut,J.,
            Fillaudeau,L. and Kallassy Awad,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2023) Faculty of Science, Saint Joseph University
            of Beirut, Mar Roukoz, Mansourieh, Beirut 1514, Lebanon
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/01/2024 03:30:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,540
            CDSs (total)                      :: 6,405
            Genes (coding)                    :: 6,082
            CDSs (with protein)               :: 6,082
            Genes (RNA)                       :: 135
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 323
            CDSs (without protein)            :: 323
            Pseudo Genes (ambiguous residues) :: 0 of 323
            Pseudo Genes (frameshifted)       :: 113 of 323
            Pseudo Genes (incomplete)         :: 238 of 323
            Pseudo Genes (internal stop)      :: 74 of 323
            Pseudo Genes (multiple problems)  :: 78 of 323
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116320.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7635
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="Lip"
                     /isolation_source="soil sample"
                     /db_xref="taxon:1428"
                     /plasmid="pLip7635"
                     /geo_loc_name="Lebanon"
                     /collection_date="2013-01-01"
     gene            join(7609..7635,1..375)
                     /locus_tag="PJW02_RS31600"
                     /old_locus_tag="PJW02_31600"
     CDS             join(7609..7635,1..375)
                     /locus_tag="PJW02_RS31600"
                     /old_locus_tag="PJW02_31600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158318.1"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="WP_000158319.1"
                     /translation="MTIYITETQAIFINETLIDMYSPNEQRGVKDTGLLQSAIYRPQQT
                     VAQEDAYPTIFHKATALFESLAKNHAFYNANKRTALACLEMFLLYNEYELKMSEQESSD
                     FTVNVVEQRLSFEEILKIIKENSSKLPLN"
                     /name="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(537..818)
                     /locus_tag="PJW02_RS31605"
                     /old_locus_tag="PJW02_31605"
     CDS             complement(537..818)
                     /locus_tag="PJW02_RS31605"
                     /old_locus_tag="PJW02_31605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002026684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_003273526.1"
                     /translation="MEYFHSYGVKFIGTVKELAKKNNLTIEETCRILELTMKIDDYDRK
                     DEQLAGIGELIKEMNDQIMGLSGGLRAISERLRDMNENVVLVSLEDKE"
                     /name="hypothetical protein"
     gene            complement(850..2055)
                     /gene="mobV"
                     /locus_tag="PJW02_RS31610"
                     /old_locus_tag="PJW02_31610"
     CDS             complement(850..2055)
                     /gene="mobV"
                     /locus_tag="PJW02_RS31610"
                     /old_locus_tag="PJW02_31610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033080.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_000033078.1"
                     /translation="MSKFMVCHMQKFKMTDVKGLQIHNQREKESHSNSDIIQERTEQNY
                     DLIHDKRKVDYKKFIQNRIDNGVVSNRAIRKDAVVCCSFMISASPDYMNSLSQAEQRRF
                     FEESVCFLKARYGEENFVYASVHVDEKTPHMHVGMVPVNEKQKLSAYSFFKNKSELHDL
                     QDKIYEHVKEKGFDIERGVSSDRKHLSTQRFKAVSLQQEIEKLEQEKKEIDSRLYDLAS
                     SLDQAKSVDEIPVKEKGGFIRSKMVEIASEDFDSIKSLAKSSESLRNENRRLKNEKIKI
                     EREKDDLYKGQRFLERQVTDLKRENRGLKEANDFLKKTLERVKEMYKEKLPELAGVIGY
                     VKGSILDKMNRKFLKRHFAGDDEVKGAQKFLNHKQEHEEQQKRLKQVRRSQQKNWDQGL
                     ER"
                     /name="mobV"
     gene            complement(2466..2666)
                     /locus_tag="PJW02_RS31615"
                     /old_locus_tag="PJW02_31615"
     CDS             complement(2466..2666)
                     /locus_tag="PJW02_RS31615"
                     /old_locus_tag="PJW02_31615"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_139252680.1"
                     /translation="MNIRISVVLPYGYSYSDSNRKCDCINVFGVFERIEKAEALPVKQK
                     NMFVEEVVISLQENSHRSVGR"
                     /name="hypothetical protein"
     gene            complement(3219..3446)
                     /locus_tag="PJW02_RS31620"
                     /old_locus_tag="PJW02_31620"
     CDS             complement(3219..3446)
                     /locus_tag="PJW02_RS31620"
                     /old_locus_tag="PJW02_31620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869766.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000869766.1"
                     /translation="MKVVNNFRVIMAVKNIDNVAELVRMTGVSRNSINKLWRNDNVSSL
                     RIDTLLPICEKLNVKLSDLIEYIPDDSETK"
                     /name="helix-turn-helix transcriptional regulator"
     gene            3804..4091
                     /locus_tag="PJW02_RS31625"
                     /old_locus_tag="PJW02_31625"
     CDS             3804..4091
                     /locus_tag="PJW02_RS31625"
                     /old_locus_tag="PJW02_31625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000323412.1"
                     /translation="MAYIISKKVKGNIYFYVAQYVGTQPYYSKQYKYKCIYAIGNQKIA
                     LERIAMWLLDNNRIPKELLEIGVSINDVKYWYEKVEKTLQNYSLTNHKNT"
                     /name="hypothetical protein"
     gene            4105..4368
                     /locus_tag="PJW02_RS31630"
                     /old_locus_tag="PJW02_31630"
     CDS             4105..4368
                     /locus_tag="PJW02_RS31630"
                     /old_locus_tag="PJW02_31630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000874966.1"
                     /translation="MAFLTNYKANGKRYFYVEKYVGKKPYTCKQSERIYSIGNERITLE
                     RLTLWILDNSFIPNELIKIGISIDDIENWREKVENTIKRYSL"
                     /name="hypothetical protein"
     gene            complement(5373..6416)
                     /locus_tag="PJW02_RS31635"
                     /old_locus_tag="PJW02_31635"
     CDS             complement(5373..6416)
                     /locus_tag="PJW02_RS31635"
                     /old_locus_tag="PJW02_31635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162903.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_000162904.1"
                     /translation="MTKKNQDEITGKLQPKKKQNIRIYEFIEKKMSESGRELFKSCSTF
                     NEFVATKDKKKKKRVKGNDCKNRFCPICAWRKAGKDAVKIATMMEAIKIEEKKEFLFLT
                     LTTPNIKADTVKSEIDRFNKAFNKLFKRRNIQRSIKGYIRKLEMTYDKERFITEEMYNN
                     KKRKAYYDSRGLKVDDHNPNYDTYNPHFHVLLCVEKNYFKRKELYIKQEEWLEMWREVT
                     DMPEITQVHIQKVELIREGNAVAEVAKYSAKDYEMSVSQDVFDVFYLALKGRQLIVYGG
                     LLKDYAKKYEDGELDKYKSTDKNEYYYRLIAMWNKDLMKFEQEYQELTESEKQEYNGHL
                     IDEMEVE"
                     /name="protein rep"
     gene            7287..7571
                     /locus_tag="PJW02_RS31640"
                     /old_locus_tag="PJW02_31640"
     CDS             7287..7571
                     /locus_tag="PJW02_RS31640"
                     /old_locus_tag="PJW02_31640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126403.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WP_000126403.1"
                     /translation="MTANTRHMETQKIERKVTKIGNSLGLTLPQEFYTTLNIKQGDVVS
                     LEINEENEQIVIKKSKKISMPEDLNPKFLESLNRTINKFDGAFKDLVNR"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     Typing          complement(5374..6551)
                     /name="rep_cluster_617"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(854..2055)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gctattttta tcaatgaaac acttatcgac atgtattcac ctaatgaaca aagaggtgtt
       61 aaagatacag ggttattaca atcagcaata taccgtccac aacaaacagt agcacaagag
      121 gatgcatatc cgactatctt ccacaaagcc actgcattat ttgaatcatt ggctaaaaac
      181 cacgcattct ataatgcaaa caaaagaacg gcattagctt gcctagaaat gtttctgtta
      241 tacaatgagt acgaattaaa aatgtccgaa caagaatctt cagatttcac tgttaatgta
      301 gtagaacaga gattatcttt tgaagaaatc ttaaaaatta taaaagaaaa ctcatcaaaa
      361 ttacctttaa attagaaaaa gacgtgggat ttaaccacgt cttttttatt aaacaaacta
      421 tcttcaagga tgttatacaa ggctctacaa tcctcgctat attatgcaaa acaagttccg
      481 ctaacgctac acatctaaag aaaaaagaga ctcacacaga gcctcctaac atgccattat
      541 tctttatctt ccaaagatac taatacaaca ttttcattca tatcacgcaa acgttcactt
      601 atagctctca gacctccact aagtcccata atttgatcat tcatttcctt gataagttct
      661 ccaattccag ctaattgttc atccttacga tcataatcat caattttcat cgtcaattcc
      721 aaaatcctac atgtctcttc aatcgttaaa ttatttttct tagccaattc ctttacagta
      781 cctataaact taactccata actatgaaaa tattccatcc ttggttgttg attaaataat
      841 gttcccacac tatctctcca atccctggtc ccaatttttc tgttggcttc ttcgtacttg
      901 ttttaatctc ttttgttgtt cttcatgctc ttgtttgtga ttcaagaact tttgagcacc
      961 ctttacttca tcatcaccag caaaatgtcg ttttaaaaac ttgcgattca tcttgtccag
     1021 tatactcccc ttaacataac caatcacccc tgcgagttca ggaagcttct ccttatacat
     1081 ctctttcact cgctccaacg ttttcttaag aaaatcattt gcttccttca accctcgatt
     1141 ctctcttttc aagtctgtta cttgcctttc taaaaaacgt tgccccttat ataaatcatc
     1201 tttttcacgt tcaatcttaa tcttttcatt tttaaggcgt ctattttcgt ttctaagaga
     1261 ctcagaagac ttagctaatg attttatact atcgaagtct tctgaagcaa tctcgaccat
     1321 tttagagcgt atgaacccgc ctttttcttt gactggaatc tcatctacac ttttcgcttg
     1381 atctaaagac gatgctaaat cataaagacg actatctatt tctttctttt cctgctctaa
     1441 cttctctatt tcttgttgca aactcaccgc tttaaaccgc tgcgtcgata aatgtttcct
     1501 gtcgctagaa acaccacgct ctatatcaaa tcccttttcc ttcacatgtt catagatttt
     1561 atcttgtaga tcatgaagct ctgatttgtt tttaaaaaat gaatacgctg atagtttttg
     1621 tttttcatta accggaacca tgcccacatg catatgaggt gttttttcat caacatgtac
     1681 tgaagcatat acaaagttct cttctccata tctcgccttt aaaaaacaaa cagactcttc
     1741 aaagaatcgt ctctgttccg cttgagataa agaattcata taatcaggac tagccgaaat
     1801 cataaacgaa caacaaacaa cagcatcttt ccgaatcgca cgattagata caacaccatt
     1861 atcaattcga ttttgaatga atttcttata atccactttt cttttatcat gaataagatc
     1921 ataattctgt tccgttcgtt cttgaataat gtctgaattt gaatgacttt ctttttcacg
     1981 ctgattatgt atttgtaatc ctttcacatc tgtcatcttg aatttttgca tatgacaaac
     2041 catgaatttt gacatgggca tcgctccttc ttcggtcgta gttccatgta aactgtagca
     2101 cactacagtt tactgcgtaa acacgtgtgc cctacgggac atttcggcga ccgccgagcc
     2161 atgacaggca tggcaaaaga attatgggct ttgcccaaac ccacaaggga aactcttccc
     2221 cttgacccct ttcaatcgaa ctccccaacc cctcaatcct tgagggactc cctcgccggt
     2281 aggcatcccc aaaacatggt tttgggaaga acaagagggt cttggtcgaa gttacctatc
     2341 aactccttaa tgctccatca tttcgctatc gcttcattcc gtccgcttac cgttgacagg
     2401 taccataaat actcctcccc cttttaaaat ctgacatcca tatattaaaa agtctctttt
     2461 tatattcacc gccccaccga tctatgggaa ttttcttgta gtgaaataac tacttcctct
     2521 acaaacatat ttttttgttt tacaggaaga gcttcagctt tttctattct ttcaaacacc
     2581 ccaaaaacat taatacaatc acattttcta ttagaatcac tataagaata gccatagggt
     2641 aaaactacag atattcgtat attcattcct agacccattc aaataaaaaa gagcataagg
     2701 aaaccgaatc ccactttgta caaaaagtgg tgaaatagac tgtgcctatt gggtttctca
     2761 tgctctactt cagggaagcg attttcccta aagtatgcgt cctctatacg tttaaaaacg
     2821 tttagtatgt attctgcatg tgctttgcat acaccatgac aaataaaaaa agagccccac
     2881 aaataggaac tcttttttca ttactcatca attccaccat ttcattttac tgcattacga
     2941 taatcacaca taaagttaaa caaaatcacg aaatatctgc ctacctctca tcttccaaga
     3001 tgttgacgca cctagcaagc ctataatttc atttaccata ttcatgtgat aacaaacaga
     3061 aaaagaaaga aaaagacagt cataaaagtt tgccgaaatt taaaataagc atataaccct
     3121 accaaaaacc atatgcgaat ataacctgtt ttccactagc aaatattaat accgatttca
     3181 tcatacacaa gctatctaaa gatagaatag cccctaaatt attttgtctc actatcgtct
     3241 ggaatatatt caattaaatc cgatagttta acatttaact tttcacagat cggtagcaat
     3301 gtatctattc gtaaagatga cacattgtcg ttacgccaca acttattgat agagttacga
     3361 cttacccctg tcatcctaac caactctgca acattatcta tattctttac cgccataatc
     3421 acacggaagt tatttactac tttcattttt tatactcctt tttctcatga aatatacatg
     3481 cttttttctt ataagggcac ttttgtgcaa agaatagttg ttttttaacc ttttaaacac
     3541 aactttagca ctttataaaa catttttaaa cctgtatgga ataaaaaatc atattatgca
     3601 ctatttcata cttcatttaa attaaagtta atttatatac atataaggtt atttatttta
     3661 tttatttaaa ctaatttaaa acctatttaa attaaaacgt tcaaaatatg cattatttca
     3721 tactttattt gttgaaaaat taacctgata aagttaatat taatcaaagt aatatagatt
     3781 tttaatgatt ggaggtcatt tctttggcat atataatatc aaaaaaagta aaaggaaaca
     3841 tttactttta tgtagcccaa tacgttggaa cacaaccata ttactcaaag caatacaaat
     3901 ataaatgcat ttacgcaatc ggcaatcaaa aaatagcttt agaaagaatc gcaatgtggc
     3961 tattagataa caaccgtata ccaaaagaac tactagaaat cggagtatcc ataaacgatg
     4021 tgaaatattg gtatgaaaaa gttgaaaaaa cattacagaa ctactcttta actaaccata
     4081 aaaacacctg aaaggagcat tactatggca tttctaacaa attacaaagc aaacggaaaa
     4141 agatactttt atgtagaaaa atatgttggt aaaaaaccat atacctgcaa acaaagtgaa
     4201 cgtatttatt cgattggaaa cgaaagaatc acattagaaa ggctcacttt atggatttta
     4261 gataacagtt tcattccaaa cgaattaata aagatcggaa tatcaattga cgatatagag
     4321 aactggagag aaaaagtaga aaacaccata aagagatact ctttataaat aaaactaaag
     4381 aaaaaagact gatattttca tcagtctttt ttctttaact atttatattt tttcatcata
     4441 aaagggttag gtattttaat aatatattta tatatttaat aaaggttacg cgtggctttt
     4501 tgaatttttc atttgcgcac aaaagcaaaa agattttaag agtaaaaagg attaaaaata
     4561 tacaggaatc acgaaataaa aaattcacag aaacaacaaa ttcaaacata atctaaacat
     4621 ctttttttat cacacaactt gtttaaaacg gtgcaacttt ttaacgatat gaaatatcta
     4681 tgaattaaat atgcgagcaa agttttttcg ttttcagttc gtcatagagg tactatataa
     4741 actccctaat ttcggaatac agaattacag agaaaacgga aaacaaaaat ttcaaaaatc
     4801 gaaaaatcaa gatttcaaat ttaaaaattt ttaaataaaa tcccacacca aaaatccaaa
     4861 atcaacccca aaaatgtaga atttctattt tcaaaaaaca tcaaaaaacg tgcggtattt
     4921 tatcgatata gatatatcta aaaaaattca tgcgcacact ataggttttt aactcgtaca
     4981 gtagtaaaac gaaatcatca gaacaaaata tatctcgaaa taaactttaa aaataaattc
     5041 acaaattcac aaatttaaat ttaagaaaaa actctttttt gttaattctc tgttagatca
     5101 taaaaacctt ttgaatgcta cttgctgcac tcagaaggtt ttctttcaat acaaaaaaga
     5161 gagaaccaaa cttaatatta agactggttc tctctttgtc attcaaatat catcaaccgc
     5221 accacatttt tcacatttaa cctagcactt atatgagtaa tatacatctt caccatcttt
     5281 gaccttactt tttgaaatcc gataaatttt catttcttcg ttataacatt cccaacaggt
     5341 atgtttaaca atatcacctt ttttatacaa aactattcaa cctccatttc atcaatcaaa
     5401 tgaccgttat attcttgttt ctcactttcc gttaactctt gatattcttg ttcaaatttc
     5461 attaaatcct tattccacat tgcaattaat ctataataat actcattttt atctgtactc
     5521 ttatacttgt ccaattcgcc atcttcgtat tttttagcat aatcttttag caatccaccg
     5581 tacacaatta attgtcgacc ttttaaagct aaataaaaca cgtcgaacac atcttgactc
     5641 actgacattt cataatcttt tgctgaatac ttcgcaacct ctgcaactgc atttccttct
     5701 cgaattaact ctactttttg aatatgtact tgcgtaatct ctggcatatc tgttacttct
     5761 cgccacattt ccaaccattc ttcctgctta atataaagct ctttacgttt aaaataattt
     5821 ttttcaacac aaagtaatac atggaaatga ggattatatg tatcataatt cgggttatga
     5881 tcgtctactt tcaatccacg agaatcataa tatgcttttc tctttttgtt gttatacatt
     5941 tcttcagtga tgaatcgttc tttatcatac gtcatttcta gctttctaat ataaccctta
     6001 atagaacgtt gaatattacg tctcttaaat aatttattaa aagctttatt aaatctatca
     6061 atctcgcttt ttaccgtatc agctttaata ttcggagttg tcaatgttaa gaaaagaaac
     6121 tctttctttt cttcaatctt gattgcttcc atcattgttg cgattttcac cgcgtccttc
     6181 cctgcttttc tccaagcaca aatcggacaa aatcgatttt tacaatcatt accctttact
     6241 cgtttctttt tctttttatc tttcgtagcc acaaactcat taaaagtact gcacgattta
     6301 aacaattctc tcccgctctc actcattttc ttctctataa attcataaat tcttatattc
     6361 tgtttctttt taggttgtaa tttaccagtg atttcatctt gatttttctt tgtcatgttt
     6421 gatatattag tcctatatga attgcttaaa aaagggtaca cgaatccctt tttcggactt
     6481 gctccttcct tggtagttat tgtttttaat tcgacactaa aaatgataaa tctacccgcc
     6541 ggaaatagca agtctttttt ttcgaaatcc cttgtgtatc aagggataaa agaatacata
     6601 ggcatcaatt tccgtgcgga aatagttacc tatgtattaa agaaagaaga aaaccgtatt
     6661 tttcgtgtcc gaaaaaccat aaaaaaatgc ccttcaatcc acttcgtgga caagggcatt
     6721 ttttctttta aaaacaaaaa ttattcctat ctctcagcct cttcattttc acttctaagc
     6781 gtttttcaaa caacttataa tattttctct ttaagaatta cacatcaatc ccctatacct
     6841 caaaaacata tatttcagaa cacgttcgaa gtgttgccat gaaaaaatcg agaaattctc
     6901 gaactttacc tgctaattag aataacaggt aactcccttt acactttctt tgtctaaata
     6961 aaacaaattc aagcaaggct ctacaatctt ccctatcttt tgcaatgaaa tataaatttg
     7021 tttacatatt tctaaaaatg agtataattg tattgacggt taatacgtta taactgttat
     7081 aacattctaa ctaaacgtca taataataca gaaagactaa acacaacaaa caaaagagaa
     7141 aactaagaag accgaaaaaa ccaaaagcaa acaaacaaaa caaacataaa atcacactat
     7201 ataattcgaa aaactcaata caaacacaaa gtggcaatta gactacgaca aaaaatagtg
     7261 tggcaaattc caaggaggaa gtccaaatga cagcaaacac tcgccacatg gaaacacaaa
     7321 aaatcgaacg taaagttact aaaatcggca attctttagg tttaacatta ccgcaagaat
     7381 tttacactac tctaaatatc aaacaaggtg acgttgtaag tttagaaatt aacgaagaaa
     7441 acgaacaaat cgttataaaa aaatccaaaa aaatttctat gccagaggat ctcaatccta
     7501 agttcttgga atcgttaaac agaaccatca ataaatttga cggtgcattt aaagatttgg
     7561 taaacagata attatccttc aggcgtggag gaaagtagga aaaccgaaat gacaatttat
     7621 ataacagaaa ctcaa
//
