LOCUS       NZ_CP116316             2189 bp    DNA     circular CON 01-MAR-2024
DEFINITION  Bacillus thuringiensis strain Lip plasmid pLip2189, complete
            sequence.
ACCESSION   NZ_CP116316
VERSION     NZ_CP116316.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32746259
            Assembly: GCF_028356555.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Fayad,N., Barssoum,R., Marsaud,N., Nasseredine,R., Teste,M.A.,
            Pailler,V., Gautier,V., Belmonte,E., Aceves,C., Cescut,J.,
            Fillaudeau,L. and Kallassy Awad,M.
  TITLE     Complete genome sequences of two Bacillus thuringiensis serovar
            kurstaki strains isolated from Lebanon and Tunisia, highly toxic
            against lepidopteran larvae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Fayad,N., Barssoum,R., Marsaud,N., Nasseredine,R., Teste,M.A.,
            Pailler,V., Gautier,V., Belmonte,E., Aceves,C., Cescut,J.,
            Fillaudeau,L. and Kallassy Awad,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2023) Faculty of Science, Saint Joseph University
            of Beirut, Mar Roukoz, Mansourieh, Beirut 1514, Lebanon
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/01/2024 03:30:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,540
            CDSs (total)                      :: 6,405
            Genes (coding)                    :: 6,082
            CDSs (with protein)               :: 6,082
            Genes (RNA)                       :: 135
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 323
            CDSs (without protein)            :: 323
            Pseudo Genes (ambiguous residues) :: 0 of 323
            Pseudo Genes (frameshifted)       :: 113 of 323
            Pseudo Genes (incomplete)         :: 238 of 323
            Pseudo Genes (internal stop)      :: 74 of 323
            Pseudo Genes (multiple problems)  :: 78 of 323
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116316.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="Lip"
                     /isolation_source="soil sample"
                     /db_xref="taxon:1428"
                     /plasmid="pLip2189"
                     /geo_loc_name="Lebanon"
                     /collection_date="2013-01-01"
     gene            70..297
                     /locus_tag="PJW02_RS27845"
                     /old_locus_tag="PJW02_27845"
     CDS             70..297
                     /locus_tag="PJW02_RS27845"
                     /old_locus_tag="PJW02_27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002100596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000572905.1"
                     /translation="MIESEGNNLKLTERQLNDLKRISELRVKLFGVPGESVVDPENVEF
                     LLDNAISSYLGQLEIFEVTIEIEQYNSMCG"
                     /name="hypothetical protein"
     gene            398..1267
                     /locus_tag="PJW02_RS27850"
                     /old_locus_tag="PJW02_27850"
     CDS             398..1267
                     /locus_tag="PJW02_RS27850"
                     /old_locus_tag="PJW02_27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001198524.1"
                     /translation="MQVYLDRLMIKYKDVTEKQFSDVLTKISSKQIFLPNTPIRSEHGT
                     SVRDYHRVIHIGYGEGAVYIGWKHNSEKEKDSYDMKVDFNPSKFENNELQKDSYEKVFE
                     TVFHTLNAVLKSNKRVVYGMDIAFDIERHMSDIVSYSKTGKQQDRHKGTVYYGNRNKDG
                     YLKIYDKKKELYNHFKRMIEEENLTRIEYSWRDSDGVVVDEIRKSPPFSIDESYTFSIF
                     NLNNVKGALKACLICYSNGTMDMKEFPRRTKESIKKALEEMDHLAVDPILQDCWLSILE
                     NIKNYTRL"
                     /name="hypothetical protein"
     gene            1455..1697
                     /locus_tag="PJW02_RS27855"
                     /old_locus_tag="PJW02_27855"
     CDS             1455..1697
                     /locus_tag="PJW02_RS27855"
                     /old_locus_tag="PJW02_27855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001293418.1"
                     /translation="MSEMVRVNTRISKKLNDWLDEYSKESGVPKSTLVHLALENYVNQK
                     VMLEQMPKMQQMLSMMFENVTQQQLNQKGNMFELK"
                     /name="hypothetical protein"
     Typing          399..1267
                     /name="rep_cluster_1434"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gatggcaaac gaaatggaat gagtgagaag agcgtacggt cggcgaggta acggaggtgt
       61 aggagcagat tgatagaaag tgagggtaac aatttgaaac tgacagaaag acaattgaat
      121 gatttgaaaa gaattagcga attacgtgta aagttgtttg gagttcctgg tgaaagtgta
      181 gttgatccag agaatgttga gtttttattg gataatgcta ttagttctta tttagggcaa
      241 ttagaaattt ttgaagtcac gatagagatt gaacagtata attcaatgtg tgggtaaatt
      301 gtagaaatgt ggcgaagaca ttttcggaca ttctaatagc cgaaaatcgt gtacaaaatg
      361 acatgtttaa taaaaaataa ggagcgggat agattttatg caagtttatt tggataggct
      421 aatgattaag tataaagatg taacagagaa acaatttagt gatgttttaa ctaaaatatc
      481 gtcaaagcag atttttttac cgaatacacc tattaggtca gaacatggga cgtctgttag
      541 agattatcat agagttatac atattggata tggtgaaggt gcagtttata tagggtggaa
      601 acataattcg gaaaaggaaa aagatagcta tgatatgaaa gttgatttta acccttctaa
      661 atttgaaaat aacgagttgc aaaaagatag ttatgaaaaa gtgtttgaaa ccgtttttca
      721 tacgttaaat gcagttttga agtctaataa gcgagtggtt tatggtatgg atattgcttt
      781 tgatatagag cgtcatatga gtgatattgt gtcttatagt aaaacaggaa agcaacagga
      841 tagacataaa ggaactgttt attatggaaa tagaaataaa gatggatatt tgaagatata
      901 tgataagaaa aaggagttat ataatcattt taaaagaatg atagaagaag agaatttgac
      961 tcgtattgag tatagttgga gagactctga cggtgtagtg gtagacgaaa taaggaagag
     1021 tcctccgttt agtattgatg aatcttatac attctcgatt tttaatttga ataatgttaa
     1081 aggggcatta aaagcttgtt tgatttgtta ttctaatgga actatggata tgaaagagtt
     1141 ccctcgtaga actaaagaga gtataaaaaa agcccttgaa gaaatggatc acttggcggt
     1201 ggaccccatt ctacaggact gttggttatc tatattagaa aatattaaga actatactcg
     1261 tttatgatat tagcgtgtgc ttctctgtgt gtcaagaggg tgtcaatatg atgctctctt
     1321 tttgtttttc ttaacttgtt tatattaata gcggatagag tcccactttt acattgttct
     1381 ggtgtatctt agtgttgata ttgtgtttaa actgatgtta tatttatgta gtacgatata
     1441 caagaggtga ttagatgagt gaaatggttc gtgttaatac acgtatcagt aaaaagttaa
     1501 atgattggtt ggacgagtat agcaaagaaa gtggtgtacc gaaaagcact ttagttcatt
     1561 tagctttaga gaattatgtg aatcaaaagg ttatgttgga acaaatgcca aagatgcaac
     1621 aaatgttgag tatgatgttt gaaaatgtaa cgcagcaaca attgaatcaa aaagggaata
     1681 tgtttgagtt gaagtaacgg ttatgtttcg aaaatgtagt cttattgatt aggagatgca
     1741 catcgataaa ctaaatgcgt agttggtgtg gctgaagttt gcccgccacc tactcattta
     1801 gaatatccgt gcatgggtcc ctgaacaatt aggaaacggc ttttgtaatc gtatgagttg
     1861 taattattat tcacttgggt gaacgttgta tgactgaaag gaaacccatg gcagttaact
     1921 gtgtaaatgg gcacttattt attaagtgta ttcgacggag ccggaagtag acgacttaag
     1981 ggagcgacat gaatgaagtg aagtgcagac agaaggtaag gtgacggagt ggactctcaa
     2041 aggacacgac ggcgcgtgaa ctgatggcaa acgaaatgga atgagtgaga agagcgtacg
     2101 gtcggcgagg taacggaggt gtaggagcag attgatagaa agtgagggta acaatttgaa
     2161 actgacagaa agacaattga atgatttga
//
