LOCUS       NZ_CP116315            15008 bp    DNA     linear   CON 01-MAR-2024
DEFINITION  Bacillus thuringiensis strain Lip plasmid pLip15, complete sequence.
ACCESSION   NZ_CP116315
VERSION     NZ_CP116315.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32746259
            Assembly: GCF_028356555.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 15008)
  AUTHORS   Fayad,N., Barssoum,R., Marsaud,N., Nasseredine,R., Teste,M.A.,
            Pailler,V., Gautier,V., Belmonte,E., Aceves,C., Cescut,J.,
            Fillaudeau,L. and Kallassy Awad,M.
  TITLE     Complete genome sequences of two Bacillus thuringiensis serovar
            kurstaki strains isolated from Lebanon and Tunisia, highly toxic
            against lepidopteran larvae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15008)
  AUTHORS   Fayad,N., Barssoum,R., Marsaud,N., Nasseredine,R., Teste,M.A.,
            Pailler,V., Gautier,V., Belmonte,E., Aceves,C., Cescut,J.,
            Fillaudeau,L. and Kallassy Awad,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2023) Faculty of Science, Saint Joseph University
            of Beirut, Mar Roukoz, Mansourieh, Beirut 1514, Lebanon
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/01/2024 03:30:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,540
            CDSs (total)                      :: 6,405
            Genes (coding)                    :: 6,082
            CDSs (with protein)               :: 6,082
            Genes (RNA)                       :: 135
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 323
            CDSs (without protein)            :: 323
            Pseudo Genes (ambiguous residues) :: 0 of 323
            Pseudo Genes (frameshifted)       :: 113 of 323
            Pseudo Genes (incomplete)         :: 238 of 323
            Pseudo Genes (internal stop)      :: 74 of 323
            Pseudo Genes (multiple problems)  :: 78 of 323
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116315.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..15008
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="Lip"
                     /isolation_source="soil sample"
                     /db_xref="taxon:1428"
                     /plasmid="pLip15"
                     /geo_loc_name="Lebanon"
                     /collection_date="2013-01-01"
     gene            353..529
                     /locus_tag="PJW02_RS27705"
                     /old_locus_tag="PJW02_27705"
     CDS             353..529
                     /locus_tag="PJW02_RS27705"
                     /old_locus_tag="PJW02_27705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015976754.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_015976754.1"
                     /translation="MSKLLTAQEVADLLRVHKKTIHRMIHSGKLDASKVANKFLIKEED
                     AKGLLENKNKMDN"
                     /name="helix-turn-helix domain-containing protein"
     gene            545..1045
                     /locus_tag="PJW02_RS27710"
                     /old_locus_tag="PJW02_27710"
     CDS             545..1045
                     /locus_tag="PJW02_RS27710"
                     /old_locus_tag="PJW02_27710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015976755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_081143358.1"
                     /translation="MENNTKQLNNEVNEIAVVDEHVNTNDIVTVDKVSPSIFEADGAKD
                     VFFSSIQSKDRKSAIKVYNAINSSENPLADHRGEVLQITDMVAHAITLEDEVTKENVDA
                     LRVVLVAKDGKAYHAISQGVVSSIQKIISIVGPAPWNEPLEIVPVEVKTRKGFKTLTLQ
                     LQG"
                     /name="hypothetical protein"
     gene            1115..1339
                     /locus_tag="PJW02_RS27715"
                     /old_locus_tag="PJW02_27715"
     CDS             1115..1339
                     /locus_tag="PJW02_RS27715"
                     /old_locus_tag="PJW02_27715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015976756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_081143359.1"
                     /translation="MNAIKNLVQVSIRTNERELEFVSFNGMVHLKQKLEDDETSVVLTK
                     EELGVVAAMSNIDNVIVNLMDSITEKKAM"
                     /name="hypothetical protein"
     gene            1398..2138
                     /locus_tag="PJW02_RS27720"
                     /old_locus_tag="PJW02_27720"
     CDS             1398..2138
                     /locus_tag="PJW02_RS27720"
                     /old_locus_tag="PJW02_27720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_087972990.1"
                     /translation="MANKRLKKKLETKRKKSLLVSEGYSKKETKKLKGRELETVYKKKS
                     HNRKNRERAREIANLAKQWGLSPSKYNSWKKLLPEIERIKKEQDGEAPFLLIYYQDFTG
                     ETDSKFIYDFKKRNSTRSRSQITKSIIGWLQNAQNKLFLGRVAMRVVPKRDVSKTNTLW
                     KNHGYVKIYVGQGKDLTKLLTAIETIMVGVYDVKERDRYLKKDLLPKLRSLPYKQAHRN
                     ADEIQKIYDVKSHGKDWWDDDGFN"
                     /name="hypothetical protein"
     gene            2101..4326
                     /locus_tag="PJW02_RS27725"
                     /old_locus_tag="PJW02_27725"
     CDS             2101..4326
                     /locus_tag="PJW02_RS27725"
                     /old_locus_tag="PJW02_27725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989234.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003887 - DNA-directed DNA polymerase activity [Evidence
                     IEA]; GO:0000166 - nucleotide binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="WP_228161272.1"
                     /translation="MGKTGGMMMVSTNRKKRREIKLFTLDTETRGLDGDVFRIGLFDGK
                     QYYTGYTFADVLPVFEKYKAYDCHVYIHNLDFDLSKIIAELRDYAEPTFDNSLFINGNI
                     VTFTASHIILHDSFRLLPSSLENLCRDFELLEDGKKDIVDYMEENHFGIYNVKNHKMNH
                     RLTKGNFFKTVDKDDPVLCEYMEYDCRSLYKILEIVIGLSNLDIEHFVNCPTTASLAKT
                     VYKEQYKKDYKVAISTKQYNHKQLGKGLEAFVRQGYYGGRTEVFTPRITDGYHYDVNSL
                     YPYVMKNAEMPVGYPNVLDSEEAQLSYDLWKRRRFGAGFIHAKVYVPEDKYIPVLPKKD
                     YTGKLIFPVGRIEGVWTFPELALAEAEGCIIEKIESGVVFEKTSPIFREFISHFEQIKN
                     SSKGAKRTFAKLMQNALYGKFAMQRERIMYADVSQREKLEAEGHTVSKIIYDMNGIYME
                     FLEYDGYAMAEYIQPHISAYITSIARIILFKGLKYAHAKGILAYCDTDSCATTTKFPDK
                     MVHDKEYGKWKLEGYVIEGLYFQPKMYAEKTIEGGKYKEVLRMKGVPKWVVEEQLDYNT
                     FKKWYLQVKRGKAEIPIYKGGERVQKFLTKSKNNIEMNELAEMHKTINFAREQKRNIDL
                     NKNITSPLVRNDYGENKDEKSEYEFDEWYERLEEFNDDMNAVEELCMKFGKIQIPEKKQ
                     RKLYGLYKEYSSKAKAMCFSNEGMPIQDWCKKTGWDMKELLGELSFL"
                     /name="DNA polymerase"
     gene            4342..4542
                     /locus_tag="PJW02_RS27730"
                     /old_locus_tag="PJW02_27730"
     CDS             4342..4542
                     /locus_tag="PJW02_RS27730"
                     /old_locus_tag="PJW02_27730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957403.1"
                     /GO_component="GO:0009289 - pilus [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FaeA/PapI family transcriptional regulator"
                     /protein_id="WP_000957403.1"
                     /translation="MLTPREQDTLECISGYMEEFGFAPSVRDMASRMYVSHKTAHRYLM
                     QLESKGRIRRVHQRPRAIQLL"
                     /name="FaeA/PapI family transcriptional regulator"
     gene            4555..4710
                     /locus_tag="PJW02_RS27735"
                     /old_locus_tag="PJW02_27735"
     CDS             4555..4710
                     /locus_tag="PJW02_RS27735"
                     /old_locus_tag="PJW02_27735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_170921274.1"
                     /translation="MRDKLLDFIIELSQSSTQVVSKAYVIDRLMQVTKEDYKELEKNAE
                     GKKGEG"
                     /name="hypothetical protein"
     gene            4700..4891
                     /locus_tag="PJW02_RS27740"
                     /old_locus_tag="PJW02_27740"
     CDS             4700..4891
                     /locus_tag="PJW02_RS27740"
                     /old_locus_tag="PJW02_27740"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021729031.1"
                     /translation="MKDDLILWLRELKVTKRDHYKTEEIISRLESIVWEEIKIHDTPSG
                     NREYNYDVQSLYPEVRKD"
                     /name="hypothetical protein"
     gene            5009..5242
                     /locus_tag="PJW02_RS27745"
                     /old_locus_tag="PJW02_27745"
     CDS             5009..5242
                     /locus_tag="PJW02_RS27745"
                     /old_locus_tag="PJW02_27745"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_087972995.1"
                     /translation="MDDFNEFKDVESCKIIIKNLIYYASLIMTKEQLLKWENSMLYDGE
                     VKEMISTMSSSLHSHQRAYKEGKQNDRHAKSN"
                     /name="hypothetical protein"
     gene            5217..5585
                     /locus_tag="PJW02_RS27750"
                     /old_locus_tag="PJW02_27750"
     CDS             5217..5585
                     /locus_tag="PJW02_RS27750"
                     /old_locus_tag="PJW02_27750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016097523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_087972997.1"
                     /translation="MIDMLKAIEENPFRVRFGDGQFFAKKKEEEDVDDIEDDEEEEEEE
                     EEKPKPKRKPKSKSEEEAPAWAQKLIDLVTPKAEQQQEAQKVPVPPKPKVEEDEEPEVE
                     EVEETDKAKKKGFLNWLF"
                     /name="hypothetical protein"
     gene            5573..6157
                     /locus_tag="PJW02_RS27755"
                     /old_locus_tag="PJW02_27755"
     CDS             5573..6157
                     /locus_tag="PJW02_RS27755"
                     /old_locus_tag="PJW02_27755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016097524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021729034.1"
                     /translation="MAVLDVPGKEAEMSEEEKKLEEKRKKDRERKARSRANTKSSSRSS
                     GKSKAPSMEILQLKPILMTVSQMVATREGFEIWALSETEINTLCEPIENMLAKTAALEK
                     VGENSDAIALAIACFTIFVPKFLMWNATRKQKKAQVVTNYAKSIQPTGNTGANKTGTTG
                     ASSGPSVGKPSTSSQAFSGELNQLIPASAPF"
                     /name="hypothetical protein"
     gene            6503..7141
                     /locus_tag="PJW02_RS27760"
                     /old_locus_tag="PJW02_27760"
     CDS             6503..7141
                     /locus_tag="PJW02_RS27760"
                     /old_locus_tag="PJW02_27760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016090421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016090421.1"
                     /translation="MQAIPTDQHVFIAGKTGSGKSFLAEVYLAGYDHVIMLDTKGQSLE
                     RRKKGENLWYGLKEGKDFVLVETLEEVAEARTKKVIYCPIPEEQDEEYYDALMKWVYER
                     HNTILWIDELMQVCPSPSKYPYHMKHLYQRGRFVDSVVWACTQRPATIPSDIMSNSTHF
                     FIFDLNKVADRKRVADDLGSDMFMDKPGYRNFWYMRDSDDEPVRATLKL"
                     /name="hypothetical protein"
     gene            7156..7296
                     /locus_tag="PJW02_RS27765"
                     /old_locus_tag="PJW02_27765"
     CDS             7156..7296
                     /locus_tag="PJW02_RS27765"
                     /old_locus_tag="PJW02_27765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016124049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023901797.1"
                     /translation="MEGKFAGVGLKNIIVLWLMFVLLTVMAKAILTKHPVRGLSEVVQA
                     V"
                     /name="hypothetical protein"
     gene            7432..7572
                     /locus_tag="PJW02_RS27770"
                     /old_locus_tag="PJW02_27770"
     CDS             7432..7572
                     /locus_tag="PJW02_RS27770"
                     /old_locus_tag="PJW02_27770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015976766.1"
                     /translation="MGKLFSPSWWVSMFINTFVTMIFIYLIKKGSDKYNIPVVRPIAEA
                     V"
                     /name="hypothetical protein"
     gene            7574..7843
                     /locus_tag="PJW02_RS27775"
                     /old_locus_tag="PJW02_27775"
     CDS             7574..7843
                     /locus_tag="PJW02_RS27775"
                     /old_locus_tag="PJW02_27775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016090418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_087972999.1"
                     /translation="MHVVTIYDGNMNRINTHFTGSKPTFEQLADFKNRYENAEYVESNE
                     RFADFEIREGERKERLANEAKQAELERIKREQGAGSGLVEGGTN"
                     /name="hypothetical protein"
     gene            7843..8913
                     /locus_tag="PJW02_RS27780"
                     /old_locus_tag="PJW02_27780"
     CDS             7843..8913
                     /locus_tag="PJW02_RS27780"
                     /old_locus_tag="PJW02_27780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015976768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016090417.1"
                     /translation="MAQQKEQYSPQQRAMIWATSTRQYKQTLPTQVVTQEGMTLDFTLP
                     KARLLTKIMLHVKAVATLKSSSGNIQRDPMSPYGILRRVELNLNNGFSPYIVSGKELFM
                     YNVLRNHPDVLLPGANKQYLNYVENVATTAGKDNEIQFTIPIPVSLNERDPVGMVMLQN
                     NTANVNLSIAVDQLANAYKLNPSNNDQVIFKSMSITPEIETFSIPSIPGGQPDMSVLKL
                     VQSKSDIFSGGGQNILKLNVGTIYRKLILYIEDLNGKPLEPKDFTGNMELVFNQADTPY
                     NIKPEILVHESHSNLGYPLPPGMYCFDFSFQGVPNLGGSRDYVDTERLTEFWFRFSTQV
                     GGKVTVVSETISRLQM"
                     /name="hypothetical protein"
     gene            8955..9185
                     /locus_tag="PJW02_RS27785"
                     /old_locus_tag="PJW02_27785"
     CDS             8955..9185
                     /locus_tag="PJW02_RS27785"
                     /old_locus_tag="PJW02_27785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523934.1"
                     /translation="MGLNHFYHDLYPDTGFNTTSGITVAEMGDQVVLVDEKDIKTDVKP
                     KSDPVTGASIWKSIGLFIIIIIAFGYVAGRL"
                     /name="hypothetical protein"
     gene            9189..9347
                     /locus_tag="PJW02_RS27790"
                     /old_locus_tag="PJW02_27790"
     CDS             9189..9347
                     /locus_tag="PJW02_RS27790"
                     /old_locus_tag="PJW02_27790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001002156.1"
                     /translation="MNEIAQFISQVGFPIFTAIFMMTKQSKDTQAMTGALTDLKIAIEK
                     NGGGKHE"
                     /name="hypothetical protein"
     gene            9421..9852
                     /locus_tag="PJW02_RS27795"
                     /old_locus_tag="PJW02_27795"
     CDS             9421..9852
                     /locus_tag="PJW02_RS27795"
                     /old_locus_tag="PJW02_27795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015976770.1"
                     /translation="MNNDAKGLTFLTLSLLFLWLVFDDFVGKKRLSKLAQMMTPDLSMP
                     SPGEVAEKVVDGAKESVKETTKNTREAQKEADKKAGEILFEKPAKNEKDPKKKEVLERL
                     SEQSKKRAETNAYKDKGWLGYSWEDLFEDTWGTVKGWFK"
                     /name="hypothetical protein"
     gene            9849..10025
                     /locus_tag="PJW02_RS27800"
                     /old_locus_tag="PJW02_27800"
     CDS             9849..10025
                     /locus_tag="PJW02_RS27800"
                     /old_locus_tag="PJW02_27800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016124056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000657808.1"
                     /translation="MKEFTESMPFVIFFMSVTVLLQSFTNERVTNGFLLLVLMSMVVTN
                     SDKFIKLLNEVRV"
                     /name="hypothetical protein"
     gene            10022..10168
                     /locus_tag="PJW02_RS27805"
                     /old_locus_tag="PJW02_27805"
     CDS             10022..10168
                     /locus_tag="PJW02_RS27805"
                     /old_locus_tag="PJW02_27805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000169698.1"
                     /translation="MTKVFDMIAGIGMLIGIYLFLSNGRETVSIIEAMGKNSIAGIKTL
                     QGR"
                     /name="hypothetical protein"
     gene            10180..10455
                     /locus_tag="PJW02_RS27810"
                     /old_locus_tag="PJW02_27810"
     CDS             10180..10455
                     /locus_tag="PJW02_RS27810"
                     /old_locus_tag="PJW02_27810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015976771.1"
                     /translation="MDYKRDNLNRHAFQALDKPVYEPSIRLNVEFDEYLGSYALLVKHT
                     AKQPKMVVADPVKEPWEKTRPNLQNEQIRREDFPQGYYFGNPIMGV"
                     /name="hypothetical protein"
     gene            10459..11073
                     /locus_tag="PJW02_RS27815"
                     /old_locus_tag="PJW02_27815"
     CDS             10459..11073
                     /locus_tag="PJW02_RS27815"
                     /old_locus_tag="PJW02_27815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015976772.1"
                     /translation="MPEFERLDPPASGGSGGKHKPKFDKKQKMLLLGGGIAVVLVALFM
                     NKAKHSGGSSQEAVEEELKDYYTNYPTLGSQNAVVQDGMNTLVGRQEELLNSILELQGK
                     KDPAELTKIYVTFDNSDEALKRQQYLINSGASTTYVRKQFIKDGWAGAKDYYVVEAYGK
                     DRQEMADLVKKGVQDKQWGGMEVGKVKTGDAPSYGAHSRSY"
                     /name="hypothetical protein"
     gene            11077..11829
                     /locus_tag="PJW02_RS27820"
                     /old_locus_tag="PJW02_27820"
     CDS             11077..11829
                     /locus_tag="PJW02_RS27820"
                     /old_locus_tag="PJW02_27820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015976773.1"
                     /GO_function="GO:0004040 - amidase activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="WP_015976773.1"
                     /translation="MALDVRPFINDAQRIQKETGIPASIILGQMVFESSGKNPGGMSGL
                     AYNAKNLFGIKGVGPAGTHTVWSQEYDAGGGRVSGFRKYNSYYESMADHARLLQTPRYA
                     SQLKNAKTFEDFARGIKAGGYATDPNYAGQLISIIKQNGLDKYDDGTPYTGEGSVPAGG
                     GSDKRGIFTSVANGVIRALLILLAFVVCVLFFAKAFPQVEATAKSGAKKVTKSSSRSRG
                     YKKVKPKGGATNGGTTGTQTKRQVEASI"
                     /name="glucosaminidase domain-containing protein"
     gene            11777..12289
                     /locus_tag="PJW02_RS27825"
                     /old_locus_tag="PJW02_27825"
     CDS             11777..12289
                     /locus_tag="PJW02_RS27825"
                     /old_locus_tag="PJW02_27825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015976774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021729042.1"
                     /translation="MAELQGLKLNDKLKQVYDKALSLGLRFTSGYRPGSTGPSGRPDSH
                     SQGMAMDFAGSKDKMDEFAKWAKTSPLFTEVLWQTAGHYDHVHVGWQEGKHQAGKMYVG
                     DKTLIDRPTGDGGGALSTGTASPNSDKGFITSAFVGIVRAVMILVFLIVAVYFFFQAFP
                     DIKVKVF"
                     /name="hypothetical protein"
     gene            12303..13196
                     /locus_tag="PJW02_RS27830"
                     /old_locus_tag="PJW02_27830"
     CDS             12303..13196
                     /locus_tag="PJW02_RS27830"
                     /old_locus_tag="PJW02_27830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015976775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176887180.1"
                     /translation="MLKDNTWREVTLTLPPKMAYTHHFIDTNPNHYDINNLSVATLYAG
                     VRVLPSLREYELLVPGNGRNILARKEGSSQIQIYNDSADAARIILTSFNEPFNPAVLAN
                     SGSVAPISNGGGGVSDTVTVKGFTSALPAGNNNIGKVVVTEMPTIPIEFKTLPSGTNMI
                     GNVGVTKLPPLAEGESHIGSVNVQGGVSISSMPPVQVTNDPVRASHFYFEAMIGTGNYV
                     CNVGDVIKFSYLVNEGDTDLFLNFDDNTLNPNLLSGKNVTIRLKPGEQLSEFTRKANKI
                     TFVRQSGNGMVRLLGV"
                     /name="hypothetical protein"
     gene            13200..14081
                     /locus_tag="PJW02_RS27835"
                     /old_locus_tag="PJW02_27835"
     CDS             13200..14081
                     /locus_tag="PJW02_RS27835"
                     /old_locus_tag="PJW02_27835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015976776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015976776.1"
                     /translation="MGLVKPFPVIETKTKRIPQTSERPLYYRVARIQSRNPVDSAEGSV
                     LQGQLFPQSNFGFTGTTQPLYTFSFGVRNGGPASLIKPSLLKVGDSREDSYRFEIYKDG
                     EGFHVLYLVLSPYSKGGVFVYHAIECKEYFEVDTEFTERGYTLVWSSVTGDTQGVYEGG
                     RRLLTESKAEELYLKKNTIGFRQVALDPATGFYHRGDGLLYTKRGDIVTLFGDLLHGNG
                     GAYKIVGRVPKEFAPLYETVIQAMYSKADSTYGSMTMIVDQAGQIIQMENRVNGDPNAT
                     NTKISGTWQCAY"
                     /name="hypothetical protein"
     gene            14098..14889
                     /locus_tag="PJW02_RS27840"
                     /old_locus_tag="PJW02_27840"
     CDS             14098..14889
                     /locus_tag="PJW02_RS27840"
                     /old_locus_tag="PJW02_27840"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016090416.1"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]; GO:0008745 - N-acetylmuramoyl-L-alanine amidase
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="WP_097938784.1"
                     /translation="MGQLKTFHAGHNYFVTGAHANGYKEEVETRRVVQRIAELCEENGI
                     HYAITTDNDGRTQRQNLNNIIANCNSHTRDRVDVAIHFNQATSEKGGVEVWYYDQYNLA
                     SKVSEAVAGALGIANRGAKQGKELAVLNGTNAPAILIEVAFLNNPGNMKAYEERFEAVC
                     RAIVQSVTGVNLKNYQDLPTMNASKYYRVNFGDFDSFDWLAGVKKRVNEILPGHGCWIA
                     CLAGNAHRILLGDYGSKEEAQAVADRLNQEFPNSGIWIEEL"
                     /name="N-acetylmuramoyl-L-alanine amidase"
ORIGIN
        1 tatgtacctc tactagccta ttaaaatatt tacctattga catgtaataa catttatgaa
       61 atatgatata caaaattttc taaaattaac tattgacaat ttggaaaatt tattggaatt
      121 tccaaaagga aaagtgtgac aagatattcc aagggtgcta taataggaaa tgagttgaaa
      181 acgaacacgc gttttataga tgttcggttt ttgtaacata actgtaacat aaaaacattt
      241 gcattactgg taactttcgg gtaatattgg tagcagagag ggacaaacgg gacaaatcga
      301 gacacgatac atactctcta tacaagtgaa aagaaggtgg tgcatatgta atatgagtaa
      361 actactgact gcgcaagagg ttgcagactt attgcgtgtt cataaaaaga ctatccatag
      421 aatgatacat tctggaaagt tagacgcttc taaggtagca aacaaattcc ttattaaaga
      481 ggaagatgca aaaggactat tggaaaataa aaataaaatg gacaattaat aggaggaata
      541 ttcaatggaa aacaatacta aacaattaaa taatgaggtt aacgaaatcg cggtagtgga
      601 cgagcatgta aatacaaatg acatcgtgac agtggacaag gtgtcaccat ctattttcga
      661 agcggatgga gctaaagatg tattcttctc atctattcaa agtaaagacc gtaaatcagc
      721 tatcaaagtt tacaatgcta tcaactctag cgaaaatccg ttagcagatc atagaggtga
      781 agttttacaa atcacggaca tggttgctca tgcgattaca ttagaagatg aagttacgaa
      841 agaaaacgtg gacgcgctac gtgttgtatt agtagcgaag gacggaaaag catatcacgc
      901 aatttcacaa ggtgtcgttt cttctattca aaagattatt agcattgtag gtccagcacc
      961 ttggaacgaa cctttagaaa tcgttccagt ggaagtaaaa acacgtaaag gttttaaaac
     1021 attaactcta caactacaag gctaattggt aacaaatgaa tgagggtaca catcgtgtat
     1081 cctctttttt aaaacacaaa aggacggtgc agacgtgaac gctattaaaa atttagttca
     1141 ggtatctatt cgaacaaatg agcgagaact agaatttgta tcttttaatg gaatggtgca
     1201 tttgaaacaa aagttagaag atgatgaaac atcggttgtg ttaacaaaag aagaattagg
     1261 ggtagttgca gctatgtcta acattgataa tgtgatagta aatttaatgg actctattac
     1321 agaaaagaaa gccatgtaag ccaaagacct gtgacctagt tgttacgggt cacatggttt
     1381 tataagtagg tgatacaatg gcaaataaac ggttaaagaa gaaattagaa accaaacgta
     1441 aaaagtcgtt acttgtttca gaaggttata gtaagaaaga aacaaagaag ttgaaaggta
     1501 gagagttaga aactgtttat aaaaagaaat ctcataatag gaaaaataga gagcgtgcta
     1561 gagaaatagc aaatctagca aaacagtggg ggctatcacc gagtaagtat aattcttgga
     1621 agaagttact tccagaaata gagagaatta aaaaagaaca agatggggaa gcgccttttc
     1681 tattaatata ctatcaagat tttacaggtg agacagatag taagtttata tacgatttta
     1741 aaaagagaaa cagtacaagg tcacgtagtc aaattacaaa gtctataata ggttggttac
     1801 agaatgcgca aaataaactc tttttaggaa gggttgctat gcgtgtagtt ccaaaacgtg
     1861 atgtatctaa aacaaataca ctatggaaga atcatggtta tgtaaagata tatgtagggc
     1921 aaggaaaaga cctaacaaaa ctactaacag caatagagac gattatggtt ggtgtgtatg
     1981 atgtaaaaga gcgtgataga tatttaaaaa aggatttatt acctaagcta cgttccttac
     2041 cgtataagca ggcgcacagg aatgcagatg agatacaaaa gatatatgat gtaaaatcac
     2101 atgggaaaga ctggtgggat gatgatggtt tcaactaata gaaaaaagcg tagagagatt
     2161 aaactcttta cgctagatac tgaaacacgt ggtttggacg gcgacgtgtt tcgaattgga
     2221 ctattcgatg gaaaacaata ctatactggc tatacgtttg cagacgtatt accagtgttt
     2281 gaaaagtaca aagcatatga ctgtcatgtg tacatacata acttagactt cgacctttct
     2341 aagatcatag cagagttgcg tgactatgcc gaaccgacat ttgataactc gctatttatc
     2401 aatggtaata tagtaacgtt taccgcttct catattatac tacatgatag ctttagattg
     2461 ctaccgtctt cacttgaaaa cttatgtaga gatttcgagc ttttagaaga tggaaagaaa
     2521 gatattgtcg attatatgga agagaatcat ttcggcatct ataatgtaaa aaatcacaaa
     2581 atgaatcatc gacttacaaa aggaaacttc ttcaaaacag tagacaaaga tgaccctgta
     2641 ttatgcgagt atatggagta tgactgtcga agtctataca agattttaga aattgttata
     2701 ggactttcaa atttggacat tgaacatttt gttaattgtc caactacggc ttcacttgct
     2761 aaaacagttt ataaggaaca gtataagaag gattataaag ttgctatttc aacgaaacaa
     2821 tacaatcata aacagttagg aaaaggacta gaagcctttg ttagacaagg ttactatggt
     2881 ggtagaacgg aagttttcac gcctagaata acagatggat accactatga tgtaaatagt
     2941 ttatatcctt atgttatgaa aaatgctgaa atgccggtgg gctatccaaa tgtacttgat
     3001 agtgaagaag cgcaactatc ttatgatcta tggaaaagaa gaagattcgg agcaggtttt
     3061 atacatgcaa aggtatacgt accagaagat aaatatatac ctgtcctacc gaaaaaagat
     3121 tatactggta agttaatttt tccagttggt agaatagaag gcgtatggac atttccggaa
     3181 cttgcattag cagaagcgga aggttgtata atagaaaaaa ttgaaagtgg cgttgtgttt
     3241 gaaaagacaa gtcctatatt ccgcgaattt atttctcatt ttgagcaaat aaagaatagc
     3301 agtaaaggcg caaagcggac atttgctaaa ctaatgcaga acgccttgta tggaaagttt
     3361 gctatgcaaa gggaacgtat catgtatgct gacgtgtcac aacgtgaaaa gcttgaggca
     3421 gaaggacata cagtaagtaa aatcatttat gatatgaatg gaatttatat ggagttttta
     3481 gagtatgacg gatatgcaat ggcagagtat atacaacctc atatttcggc ttatattact
     3541 tctattgcta gaattatatt gtttaaaggt ttgaagtatg cacatgcaaa agggattctt
     3601 gcgtattgtg atacggacag ttgcgcaaca actacaaagt ttccggacaa gatggtacac
     3661 gacaaagaat atgggaaatg gaagctagag ggatatgtta ttgaggggct atattttcaa
     3721 cctaaaatgt atgctgaaaa aacaattgag ggtggaaagt ataaggaagt attacgaatg
     3781 aaaggcgttc cgaaatgggt agtagaggaa caactagatt ataatacgtt taaaaaatgg
     3841 tacttacaag taaaaagagg aaaagcggaa attcctattt ataaaggcgg agaacgtgtc
     3901 caaaagtttt taacgaaatc taaaaataat attgaaatga atgaactagc ggaaatgcat
     3961 aagacaatta attttgctag ggagcaaaaa agaaatattg atctaaataa aaatatcaca
     4021 tcacctctag ttcgtaatga ttatggggaa aataaggacg aaaagtcaga atacgagttt
     4081 gatgaatggt atgaaagatt ggaagagttt aacgatgata tgaatgcggt agaggaactt
     4141 tgtatgaagt ttggaaagat acaaatacca gagaaaaaac aaagaaagtt atacggttta
     4201 tataaagagt attcttcaaa agcgaaagcc atgtgcttct ctaatgaggg aatgccaatc
     4261 caagattggt gtaaaaagac aggatgggac atgaaagaac tattaggcga attaagtttc
     4321 ttataatagg gagtgacgaa aatgttgacg ccaagggaac aggatacgtt agaatgtata
     4381 agcgggtata tggaagagtt tgggttcgcg ccgagcgtgc gagatatggc gagtcgaatg
     4441 tacgtaagtc ataagacagc acatcgctat ttaatgcaat tggaaagtaa aggacgaatt
     4501 aggagagtac atcaaagacc tagagccatc cagttgttat aaggagggaa aggtatgcgt
     4561 gacaaattgc ttgactttat catcgaacta tcacaatcta gcacacaagt cgtaagcaag
     4621 gcatatgtca tcgacaggtt aatgcaagta acaaaagaag actacaagga actagaaaag
     4681 aatgcggagg ggaagaaggg tgaaggatga tttaatattg tggttacgtg aactaaaggt
     4741 cacgaaaaga gatcactata aaacagaaga aataattagt agattagaaa gtatcgtttg
     4801 ggaagaaata aagatacatg atacacctag cggaaataga gaatataact atgatgtaca
     4861 aagtttatac ccagaggtaa ggaaggacta gcaaagttgc tagtcttttt tatttgtcca
     4921 aaatgacaca cgtgtgacgt tatgaggaac actcgttcgt attatagtat acatatacca
     4981 aacaaacatt cgataaggag tgagatacat ggacgacttt aacgaattta aagatgtgga
     5041 atcttgcaaa attattataa aaaatcttat ttattatgcg tcattgatta tgacaaaaga
     5101 gcaactatta aaatgggaaa attccatgct atacgatggt gaagtgaaag aaatgattag
     5161 caccatgtca tctagtttgc atagtcatca acgcgcatat aaggagggaa aacaaaatga
     5221 tagacatgct aaaagcaatt gaagaaaatc cctttcgagt acgattcgga gacggacaat
     5281 tcttcgccaa aaagaaggaa gaggaagacg ttgacgacat cgaagacgat gaggaagagg
     5341 aagaagagga agaagaaaag ccgaaaccaa aacgcaaacc aaaatcaaaa agcgaggagg
     5401 aagcaccagc atgggcacaa aagttgatag acttagtgac gccgaaagcg gagcaacaac
     5461 aggaagcgca aaaggtacca gtaccaccga aaccaaaagt agaggaagac gaggagccgg
     5521 aagtggagga agtggaggaa acggacaagg cgaagaaaaa gggcttccta aattggctgt
     5581 tttagacgtt cccggtaaag aagcggaaat gtctgaagag gaaaagaagc tagaagagaa
     5641 gcgcaaaaag gacagggaac gaaaagcccg ttcgcgcgcg aatacaaaat cttcttcccg
     5701 atctagcggg aaatcaaaag caccgtcaat ggaaatcttg caattgaaac caattctaat
     5761 gacagtgtca caaatggtgg ctacccgtga agggtttgaa atatgggctt taagtgaaac
     5821 agaaattaat acgctatgtg aaccgataga gaacatgtta gcaaaaaccg cagcactcga
     5881 aaaagtcggg gaaaatagtg acgcgattgc tcttgctatc gcatgcttta caatctttgt
     5941 accaaaattc ttaatgtgga atgccacaag aaaacagaag aaagcgcagg tggtaacaaa
     6001 ttatgctaaa tcaattcagc ctaccggaaa caccggggca aacaaaacag gaacgactgg
     6061 agcaagtagc ggaccaagtg ttggaaaacc ttccacttcc agtcaagctt ttagcgggga
     6121 actcaatcaa ctcattccgg caagcgctcc attttgagga ctttgacgaa caaatcgaca
     6181 atggacttga aaagctgaaa gaagtgattg actatgtcca atacggtact tatccgggcg
     6241 agtaggtgta tctattctat tccgcttgat gattggcgta caaattggtg tccgtgctta
     6301 ccaaaactgg acgagtggtt tgtaacgatt ggtgagaaat atgtggtctt tgaatacggc
     6361 gatatttgcc accgtatgga tgagcattat ttcaacacgt tactaccacg tatcccctat
     6421 tcgtcacgat ttaaggcgtt acgaaatgga aaaggtgtat tgcgcacgga aaggagttac
     6481 agcaagaagg gaatgaagaa acatgcaagc aatcccgact gaccaacatg tttttatagc
     6541 tggtaaaaca ggtagcggaa aatcgtttct tgctgaggtt tacctagccg ggtacgacca
     6601 tgtgattatg ttagatacga aaggacagtc acttgagaga cggaagaaag gcgagaacct
     6661 atggtatggg ttaaaggaag ggaaagactt tgtactggtc gagacactgg aagaagtagc
     6721 cgaagcccgc acgaaaaagg taatttattg tccgattccg gaagagcaag atgaagagta
     6781 ttatgacgcg cttatgaaat gggtgtatga gcgacataac acaattttat ggatagacga
     6841 gttaatgcaa gtttgtccaa gtccaagtaa gtatccgtat catatgaaac acctctatca
     6901 gcgaggacgc tttgtggatt ccgtcgtatg ggcatgtaca caaagacctg caacgattcc
     6961 atcggatatt atgtccaact ctacacactt ctttattttt gatttgaata aagtggcgga
     7021 tagaaaacgt gttgctgatg atttggggtc tgatatgttt atggacaaac cgggttatcg
     7081 taatttctgg tatatgcggg atagcgatga cgaacctgtc cgcgccactt tgaaactttg
     7141 aaagggggtg acaatgtgga gggtaaattt gcaggggtcg gactcaaaaa tattattgtg
     7201 ttatggctca tgtttgtact gttaacagtc atggcgaaag caattttaac aaaacatccg
     7261 gttcgtggac ttagtgaagt tgtccaagcc gtataggagg tgtgacatgc atttattaga
     7321 agttattgtt ccgacggcta tgtcgtcatt tgctctaggg attatagtag gagcgaacat
     7381 catgaattgc aatggattta agaaaaagta agaaaaagga ggaaaagaaa aatgggaaaa
     7441 ctatttagcc cgtcatggtg ggtatctatg tttatcaaca catttgtgac aatgattttc
     7501 atttatttaa ttaaaaaagg ttctgataag tataatattc cggttgtaag accgattgcg
     7561 gaggcggtat aacatgcacg tagttactat ttatgatgga aatatgaatc gtattaatac
     7621 acatttcacg ggaagtaaac caacatttga gcaacttgct gatttcaaaa atcgttatga
     7681 aaatgcggaa tatgtggaat caaatgaacg ttttgctgac tttgaaattc gtgaaggcga
     7741 acgaaaagaa cgtttagcga atgaagcaaa gcaagcggaa ctggaacgaa tcaaacgtga
     7801 acaaggcgcg ggaagcggac ttgttgaagg aggaacaaac taatggcaca acagaaagag
     7861 caatattcac cgcaacaacg cgcaatgatc tgggcgacgt caacacgaca atacaaacaa
     7921 acattaccaa cgcaagttgt tacacaagaa ggtatgacgc ttgatttcac attaccgaaa
     7981 gcgcgtctat taacaaaaat catgctacat gtaaaagccg tagccacgtt gaaaagctct
     8041 agcgggaaca ttcaacgcga cccaatgtct ccatatggta ttttacgccg agtagaactc
     8101 aacttaaata acggcttcag tccttatatt gtatcaggga aagaactgtt tatgtataac
     8161 gttttacgta atcatccgga cgtattgtta ccaggtgcga ataaacaata cttaaactat
     8221 gtggaaaacg tagcaacaac agccgggaag gacaatgaaa ttcaatttac aatcccaatc
     8281 cctgttagtt taaatgaacg tgacccggtc ggaatggtta tgctgcaaaa caacacggct
     8341 aacgtaaact tatcaattgc cgttgaccaa ttagcaaatg catataaatt aaatccatca
     8401 aataatgacc aagtcatatt taaatctatg tccattacac cagagatcga aacattctct
     8461 attccgtcga ttccgggcgg gcaaccggat atgtctgtac tgaaactcgt acaatcgaaa
     8521 tcggacattt tcagtggcgg tggacaaaac attttaaaac taaacgtagg gacgatttat
     8581 cgtaaactca tactctacat cgaagactta aacgggaaac cattagagcc aaaagacttc
     8641 acgggtaata tggaacttgt ctttaaccaa gcggacacgc cttataatat caagccggaa
     8701 atactcgtac acgaaagtca tagtaatcta ggctatccac ttccaccggg tatgtattgc
     8761 tttgacttct cattccaagg tgtaccaaac ctcggagggt cacgcgacta tgtggatacg
     8821 gagcgtttaa cagaattctg gttccgcttc agcacgcaag taggcgggaa agtcactgtc
     8881 gtatcggaaa caatatcacg actacaaatg taaggggctt tatgctcctt acctatttat
     8941 aaggagggaa cagaatggga ctgaatcatt tctatcacga tctatacccg gatacgggtt
     9001 tcaatacgac aagtggaatc accgtagcgg aaatgggtga ccaagttgta ttagtggatg
     9061 aaaaggacat caagacagat gtgaaaccga agtccgaccc agtgacaggt gcaagcattt
     9121 ggaagtccat tggtttattc atcattatca taatcgcatt cggttatgtg gcggggaggt
     9181 tgtaagtcat gaatgagatt gctcaattca ttagtcaagt gggattcccg attttcacgg
     9241 ctatttttat gatgacaaaa caatcaaagg atacacaagc tatgacgggt gcgttaactg
     9301 atttaaagat agcaattgaa aagaatggtg gcggtaagca tgagtaaatc attaatccta
     9361 gttgttgcca ttcttgcttt atggttcttt gtaattcgaa agaaaaaggc gtgatgacat
     9421 gtgaacaacg acgcgaaagg tttaacgttt ctcactctat cccttttatt tttatggctc
     9481 gtgtttgacg actttgtcgg aaagaaacgt ctatcaaaat tggcgcaaat gatgacgccg
     9541 gacttatcaa tgcctagccc gggggaagtt gctgagaaag tggtggacgg tgcgaaagag
     9601 tctgtaaaag aaacaacaaa gaatacaagg gaagcgcaaa aagaagcgga taaaaaagcg
     9661 ggtgaaattc tgtttgaaaa acctgcaaag aatgaaaaag accccaaaaa gaaagaagtg
     9721 ttagaacgtc tttccgaaca gagcaagaaa cgtgccgaaa cgaacgcgta taaagataag
     9781 gggtggctcg gatacagttg ggaggactta ttcgaggata cgtggggtac ggtgaaaggg
     9841 tggtttaaat gaaagaattt acggagtcaa tgccattcgt catcttcttt atgtccgtga
     9901 ccgtcctatt gcaatccttt acgaacgagc gtgtaacaaa tggatttttg ctactcgtat
     9961 taatgagtat ggttgttaca aattcggaca agtttataaa actattaaat gaggtgagag
    10021 tatgacaaaa gtattcgaca tgatcgcggg aattggaatg ctcattggta tttatttgtt
    10081 cctttcaaat ggtcgagaaa cagtgtcaat cattgaagcg atgggtaaaa actcaattgc
    10141 gggcattaaa acattacaag gacgatagga gggttttata tggattacaa gcgcgacaat
    10201 ttaaatcgtc acgcgttcca agcgttggac aagcccgtat atgaaccctc tatcagattg
    10261 aacgtggagt ttgacgagta cctcggctct tatgcgttac tggtgaagca tacagcaaag
    10321 cagccaaaaa tggttgtggc tgacccggta aaagaaccgt gggagaaaac acgaccaaac
    10381 ttgcaaaacg aacaaatccg aagagaggat ttcccgcaag gctactattt cgggaatccc
    10441 attatggggg tgtagggaat gccagaattt gaaagactcg accctcctgc aagcggtggg
    10501 agtggcggaa aacataaacc gaagtttgac aaaaaacaaa agatgctatt actaggaggc
    10561 ggaattgccg tcgtcctagt ggctcttttt atgaataagg caaagcatag cggtggttca
    10621 tcgcaagaag ccgtagagga agaattgaag gactactata caaactatcc aacgctcggt
    10681 agtcaaaatg ctgttgtgca agatggaatg aacacgcttg tcgggcgaca agaagaattg
    10741 cttaattcca ttttagaact gcaagggaag aaagaccctg ctgaactaac aaagatttat
    10801 gtcacattcg ataacagtga tgaagcgtta aaacgtcaac aatatttaat taattctgga
    10861 gcaagtacga catatgtcag aaaacaattc attaaagatg gatgggcggg cgcgaaggat
    10921 tactatgttg tggaagcata cggaaaagac cgtcaagaaa tggctgacct tgtgaaaaaa
    10981 ggtgtccaag acaaacaatg gggcggaatg gaagtcggga aagtgaagac cggggacgca
    11041 ccaagctatg gcgcacattc taggagttat taattaatgg ctctagatgt tagaccattc
    11101 ataaacgacg cccaacgaat ccaaaaagaa actggaatcc ctgcttctat tattctcggt
    11161 caaatggtgt ttgaatctag cggaaagaat cccggcggaa tgtccggtct tgcctataat
    11221 gctaagaatc tatttggcat taaaggtgtc ggacctgccg gaacacatac tgtatggtca
    11281 caagagtatg atgcgggcgg tggtcgtgtt tctggattcc ggaaatataa cagctactat
    11341 gagtcgatgg ctgaccatgc tcggttgctg caaacaccgc gctatgcgtc ccaactaaaa
    11401 aatgcgaaga catttgagga ctttgcgcgc gggattaagg cgggcggata cgcgactgac
    11461 cccaactatg cgggacaatt gatatctatt attaagcaaa acggattaga caagtatgac
    11521 gacgggacgc catacaccgg agagggaagc gttcctgccg gtggagggag tgataaacgt
    11581 ggaatattca caagtgtcgc taatggagtc attcgcgcct tgcttatctt attggctttt
    11641 gtggtttgtg ttctcttttt tgccaaggca ttcccacaag tggaagctac ggcaaagagc
    11701 ggagcaaaga aagttacaaa gtcatcctca cgatctagag gatataaaaa agtaaaaccg
    11761 aaaggcggtg ctacgaatgg cggaactaca gggactcaaa ctaaacgaca agttgaagca
    11821 agtatatgac aaggcgttat cccttggttt gcgattcaca agcggatacc gccccggttc
    11881 taccggacca agcgggagac cggatagcca ttcccaaggc atggctatgg acttcgcggg
    11941 ttcgaaagat aaaatggacg aatttgcgaa atgggcgaaa acgtcaccgc tatttacgga
    12001 agtgttatgg caaactgctg gacactacga tcatgtacac gtcgggtggc aagagggaaa
    12061 acaccaagcc ggaaagatgt acgtaggcga taaaacattg atcgacaggc ccacgggtga
    12121 cggtggcggt gccctaagca cggggacggc ttccccaaat tccgacaaag gatttatcac
    12181 aagtgcattt gttggaatcg ttcgtgctgt tatgattctt gtctttctga tcgtagctgt
    12241 atatttcttc ttccaagcat tcccggatat aaaagtaaaa gtattttaag gagttgaaaa
    12301 acatgttaaa ggataataca tggcgtgagg tcacgctcac actaccgcct aaaatggcgt
    12361 atacacatca tttcattgat acaaacccaa accattatga cattaacaat ctatctgtag
    12421 cgacgctata cgcgggcgtt cgtgtcctac caagtctaag agaatacgaa ttacttgtac
    12481 cgggtaatgg acgaaacatt ttagcaagga aagaaggttc ttcccaaatc caaatttata
    12541 acgatagtgc ggatgctgca agaatcattt taacatcgtt taacgaaccg tttaaccctg
    12601 ctgtactagc aaatagcgga agtgttgccc cgatttcaaa cggtggcggt ggggtaagtg
    12661 ataccgtcac ggtaaaagga tttacaagcg cgttacctgc tgggaataac aatataggta
    12721 aagtagttgt cacggaaatg cctaccattc caattgagtt taaaacatta ccatcgggta
    12781 caaatatgat tggtaacgta ggtgtcacga aactaccgcc acttgctgaa ggtgaaagtc
    12841 atatcggaag tgtgaatgta caaggtgggg tttcgatttc cagtatgcca cctgtacagg
    12901 ttacgaatga cccggtgcga gcaagtcact tctatttcga agcaatgatc gggacaggga
    12961 actatgtatg caatgtaggt gacgtcatta aattctcgta cttagtgaat gaaggtgaca
    13021 ccgatctatt cctaaacttt gatgataata cattaaatcc aaatttatta tccggtaaga
    13081 atgtaaccat tcgtttgaaa cccggtgaac aattatcaga gtttacgaga aaagcaaata
    13141 aaataacatt tgtgcgccaa tccggaaatg gaatggttcg gctgttaggg gtgtaataga
    13201 tggggcttgt taaaccattt ccagttatag aaacaaaaac aaagaggata ccgcaaacga
    13261 gcgaaagacc tctttattat agggttgcta gaatccaatc aagaaaccca gttgatagcg
    13321 cggaagggtc tgttcttcaa ggacaacttt tcccgcaaag taactttgga ttcacgggta
    13381 caacgcaacc gttatacacc ttttcatttg gtgttcgaaa cggcgggccc gcttcactta
    13441 tcaaaccatc cctactaaaa gtaggagatt cccgcgaaga tagttaccgt tttgaaatat
    13501 acaaagacgg ggaagggttt catgttcttt atcttgttct atctccctat tcaaagggtg
    13561 gtgtattcgt ctatcatgca attgaatgta aagagtattt tgaggtggac acggagttta
    13621 ccgaacgtgg atataccctt gtttggtcaa gtgtaacagg tgatacacaa ggcgtatatg
    13681 aaggcgggcg aagactatta acggaaagca aagccgaaga actctatttg aaaaagaata
    13741 caattggatt ccgtcaagtg gcactagacc ccgcgacagg tttttaccat cgtggtgacg
    13801 ggttacttta taccaagcga ggggacattg taacactatt cggtgacctg ctacatggta
    13861 acggtggtgc ttataaaatc gtgggacgtg taccgaaaga gtttgccccg ttatacgaaa
    13921 cagttataca ggctatgtat tcgaaagctg attccacata tggaagtatg acaatgatcg
    13981 tagaccaagc cggacaaatt atacaaatgg aaaatagggt gaatggtgac ccgaacgcaa
    14041 caaataccaa aatcagtggc acatggcaat gtgcatatta aagggaggaa aattagaatg
    14101 ggacaattaa aaacttttca cgcggggcat aactactttg tgacaggagc acatgcaaat
    14161 gggtacaagg aggaagtcga aacaagacga gtcgtgcaaa gaatcgctga attatgcgaa
    14221 gagaacggaa ttcactatgc aatcactaca gacaacgacg gacgcacaca acgccagaat
    14281 ttaaacaaca tcatagcgaa ttgtaacagc catacaagag atcgtgtgga cgtagcgatt
    14341 cacttcaacc aagcaacgtc agaaaaaggc ggtgtcgagg tttggtatta tgaccaatat
    14401 aatttagctt ctaaagtgag cgaagccgtg gcgggtgcgc taggtatcgc aaaccgtgga
    14461 gcgaagcaag ggaaggaatt agcggttctg aatgggacaa atgctcccgc tattttaata
    14521 gaggttgcat tcttaaacaa tccggggaat atgaaagcgt acgaggaacg atttgaagcc
    14581 gtttgccgtg cgattgtgca aagtgtgaca ggtgtgaatc ttaaaaacta tcaggactta
    14641 ccaacgatga atgcgagcaa atattaccgt gtaaacttcg gtgactttga tagctttgat
    14701 tggttagcgg gtgttaagaa acgtgtaaac gaaatattac caggtcacgg ttgttggatt
    14761 gcttgcctag cggggaacgc tcatagaatc ctattaggtg actatggaag taaagaggaa
    14821 gcacaagcgg ttgctgatag attgaaccaa gagtttccga atagtggaat atggatagag
    14881 gaattataaa ataggaaaga gttatggaat tgattccatg actctttttt attttgttta
    14941 tatcgtggaa catagatgtt tatttgtgtc aatgggtaat attggtaaaa gtgttagtag
    15001 ggatacat
//
