LOCUS       NZ_CP116314            12276 bp    DNA     circular CON 01-MAR-2024
DEFINITION  Bacillus thuringiensis strain Lip plasmid pLip12, complete sequence.
ACCESSION   NZ_CP116314
VERSION     NZ_CP116314.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32746259
            Assembly: GCF_028356555.1
KEYWORDS    RefSeq.
SOURCE      Bacillus thuringiensis
  ORGANISM  Bacillus thuringiensis
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1  (bases 1 to 12276)
  AUTHORS   Fayad,N., Barssoum,R., Marsaud,N., Nasseredine,R., Teste,M.A.,
            Pailler,V., Gautier,V., Belmonte,E., Aceves,C., Cescut,J.,
            Fillaudeau,L. and Kallassy Awad,M.
  TITLE     Complete genome sequences of two Bacillus thuringiensis serovar
            kurstaki strains isolated from Lebanon and Tunisia, highly toxic
            against lepidopteran larvae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12276)
  AUTHORS   Fayad,N., Barssoum,R., Marsaud,N., Nasseredine,R., Teste,M.A.,
            Pailler,V., Gautier,V., Belmonte,E., Aceves,C., Cescut,J.,
            Fillaudeau,L. and Kallassy Awad,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2023) Faculty of Science, Saint Joseph University
            of Beirut, Mar Roukoz, Mansourieh, Beirut 1514, Lebanon
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: flye v. 2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/01/2024 03:30:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,540
            CDSs (total)                      :: 6,405
            Genes (coding)                    :: 6,082
            CDSs (with protein)               :: 6,082
            Genes (RNA)                       :: 135
            rRNAs                             :: 8, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 106
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 323
            CDSs (without protein)            :: 323
            Pseudo Genes (ambiguous residues) :: 0 of 323
            Pseudo Genes (frameshifted)       :: 113 of 323
            Pseudo Genes (incomplete)         :: 238 of 323
            Pseudo Genes (internal stop)      :: 74 of 323
            Pseudo Genes (multiple problems)  :: 78 of 323
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116314.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12276
                     /organism="Bacillus thuringiensis"
                     /mol_type="genomic DNA"
                     /strain="Lip"
                     /isolation_source="soil sample"
                     /db_xref="taxon:1428"
                     /plasmid="pLip12"
                     /geo_loc_name="Lebanon"
                     /collection_date="2013-01-01"
     gene            complement(366..701)
                     /locus_tag="PJW02_RS27600"
                     /old_locus_tag="PJW02_27600"
     CDS             complement(366..701)
                     /locus_tag="PJW02_RS27600"
                     /old_locus_tag="PJW02_27600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176887196.1"
                     /translation="MFARYRSQDDEFMSQLQFLALGAIMTFLIKFGCISPEVNMLTLFV
                     LFVILIGIYYLFVLLLTKYFYRFLSKQHDKLHAYLCNKYGEKIANRAGLSFLVILIVGM
                     LIICLLI"
                     /name="hypothetical protein"
     gene            complement(713..1132)
                     /locus_tag="PJW02_RS27605"
                     /old_locus_tag="PJW02_27605"
     CDS             complement(713..1132)
                     /locus_tag="PJW02_RS27605"
                     /old_locus_tag="PJW02_27605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RND transporter"
                     /protein_id="WP_176887194.1"
                     /translation="MFEKFYREYIHVLFNIVAMFFYFSWCLVGAKFNLLTLIAIGIALF
                     IFVKYVSMDKKFVNENIDKLKDKKMIFRKYAISGENIGYAVFTNFLLFLLYLGISVYFK
                     DLSVISLLFAFILNVSFLMLTLRLMSKFSLKNSEV"
                     /name="RND transporter"
     gene            complement(1154..1351)
                     /locus_tag="PJW02_RS27610"
                     /old_locus_tag="PJW02_27610"
     CDS             complement(1154..1351)
                     /locus_tag="PJW02_RS27610"
                     /old_locus_tag="PJW02_27610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228161285.1"
                     /translation="MDLPICNEKEVTVLSKTLKYLRSDQSNQVKVHKEYLEDRGWIFDD
                     LSYIDSHIYVVYKKRGCEAK"
                     /name="hypothetical protein"
     gene            complement(1447..1869)
                     /locus_tag="PJW02_RS27615"
                     /old_locus_tag="PJW02_27615"
     CDS             complement(1447..1869)
                     /locus_tag="PJW02_RS27615"
                     /old_locus_tag="PJW02_27615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003308735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="WP_176887192.1"
                     /translation="MEQIYIGQLQNIFSTVIFNIALILGCIVVAIMIRAIKKLFIGDGY
                     SYSSSRKGYDYSPTVNLNKSSYSNVGNKKSNDSSSKKTSSSSSSNSDYLIATSLAASSY
                     SSYDSSSSYDSGCSSSSYDSGSSSSYDSGSICGFGD"
                     /name="RNA-binding protein"
     gene            complement(2541..2732)
                     /locus_tag="PJW02_RS27620"
                     /old_locus_tag="PJW02_27620"
     CDS             complement(2541..2732)
                     /locus_tag="PJW02_RS27620"
                     /old_locus_tag="PJW02_27620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000511845.1"
                     /translation="MGILKKKRFREEVKRINKAHGEMREFLDLLMDRYGLDEEEINNCE
                     VIKHHFDNLDVMFSQMAK"
                     /name="hypothetical protein"
     gene            complement(2803..3405)
                     /locus_tag="PJW02_RS27625"
                     /old_locus_tag="PJW02_27625"
     CDS             complement(2803..3405)
                     /locus_tag="PJW02_RS27625"
                     /old_locus_tag="PJW02_27625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001102046.1"
                     /translation="MNVMVFEGPLGSGKTLGMTLFAHHFKQKSNCVLYSNYGVVGSKPF
                     TEIEHFKNIAQEKSTILNLDEAHIDLDARSFSSNSVKFFSQVSYYLRKLRCTLFIASPS
                     FDDLDARIRGITNVLVKVSSDKKYFYYTMYDIQSKRYLKRMRISKKKAFVVGSKIYDTS
                     AMVSPVKVPEKRQDFMEFLEALKTTAEEYGRQYKHSA"
                     /name="hypothetical protein"
     gene            complement(3402..3809)
                     /locus_tag="PJW02_RS27630"
                     /old_locus_tag="PJW02_27630"
     CDS             complement(3402..3809)
                     /locus_tag="PJW02_RS27630"
                     /old_locus_tag="PJW02_27630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015484804.1"
                     /translation="MSEVTLEKTVVEEVLPDVQDIMKEEILEEDTSSESIETVQLFQYM
                     KENSVPLNASQMKGILLLNENGCKDIADYVISVKKQSLPLAFYEKVLKILTMFDRIKGN
                     AKLSHLLKANANPSLSLSANSAKVVKGNELA"
                     /name="hypothetical protein"
     gene            complement(3823..4185)
                     /locus_tag="PJW02_RS27635"
                     /old_locus_tag="PJW02_27635"
     CDS             complement(3823..4185)
                     /locus_tag="PJW02_RS27635"
                     /old_locus_tag="PJW02_27635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_151624126.1"
                     /translation="MLAKTKRKVQPQFEGHRALIIKEEGTCVVEQVISFDSEILETSTA
                     MLPRENELRLLDDNHGYVYHIYNLEKPARIEAENIKRLRRSSALKGIFEFETGKKQFDL
                     IGFMPWVIIVLLAIFK"
                     /name="hypothetical protein"
     gene            complement(4492..4752)
                     /locus_tag="PJW02_RS27640"
                     /old_locus_tag="PJW02_27640"
     CDS             complement(4492..4752)
                     /locus_tag="PJW02_RS27640"
                     /old_locus_tag="PJW02_27640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000992627.1"
                     /translation="MNALIDTIFNPVLGWLAQMAKYLNDLSVPASRPLRISDYLGVFAY
                     LYGWTTVITTLFALVFVYFVCYIIMTYSSLFIRFKDTVKWW"
                     /name="hypothetical protein"
     gene            complement(4752..5177)
                     /locus_tag="PJW02_RS27645"
                     /old_locus_tag="PJW02_27645"
     CDS             complement(4752..5177)
                     /locus_tag="PJW02_RS27645"
                     /old_locus_tag="PJW02_27645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015484803.1"
                     /translation="MMEKFVKFLKGGFEAVIKSLKTFIDFLATPFQYLLDFLKGIFYFI
                     EKLFQVVVAIIKLFVALFQFLGAVIAGFFKTLFLWVQPNFDGGNVSFPSASMKGFKTAT
                     DILLPTGLLTVVPMVVTCFIWYLFVKKLMDLFRGGHH"
                     /name="hypothetical protein"
     gene            complement(5174..5329)
                     /locus_tag="PJW02_RS27650"
                     /old_locus_tag="PJW02_27650"
     CDS             complement(5174..5329)
                     /locus_tag="PJW02_RS27650"
                     /old_locus_tag="PJW02_27650"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015386302.1"
                     /translation="MIQPVTTKDLIQFLQYAFHAGEDVLFLAFVLMITFSVAVSIRRIM
                     VGYKQS"
                     /name="hypothetical protein"
     gene            complement(5339..6766)
                     /locus_tag="PJW02_RS27655"
                     /old_locus_tag="PJW02_27655"
     CDS             complement(5339..6766)
                     /locus_tag="PJW02_RS27655"
                     /old_locus_tag="PJW02_27655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016090439.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="WP_228161292.1"
                     /translation="MLACSEWKMWIKKFFIVVPVLLGILFVGDTSAFAQGKDVLSGRYG
                     KDRFGTIVSTLTDGNLRTSDTLANGEITFTLQSPVRVSGFKFTKVGGSSNYMNVYFYDT
                     EGRVLKKGYSKTDSGYVNPIDIKGVTKVVLENTSSGDYFTISEVNLYEYESVPPSDIAD
                     LKENIKHDSVEFTYKNPKSNFSYLRIYRDGQVLASDVKEEKFTDKGLTPETEHTYKFVS
                     VSPDGLESKGIEKKIKTAKEPLPDEIKDLQEDEITHTYVKFSYKFPKNNFDHVKIYRDD
                     KVIADNVKIEEFVDKGLKGSTVYTYKFVSVSSTGLESNGIVKKVQTDEEPNLNKPSKPS
                     NFDITPKDGSLIVNLLSQNLGVPIKGYHIFIDGVKVNDSLVTSRSYAVKGLQNGQTYGV
                     QIKAVSKWNIESDLSNVVNGIPKVPVIPEVTFSFGLGALVDGITNWFLGIWPIVAFSIA
                     IPLAFIVAFNTKKLFMR"
                     /name="fibronectin type III domain-containing protein"
     gene            complement(6733..8112)
                     /locus_tag="PJW02_RS27660"
                     /old_locus_tag="PJW02_27660"
     CDS             complement(6733..8112)
                     /locus_tag="PJW02_RS27660"
                     /old_locus_tag="PJW02_27660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_176887190.1"
                     /translation="MKKAAIIFLCTFCIIFRTVPVFADTPSFQMVGSKGVSIENKGLVG
                     GYYSLSLKISDAKDVVTFQLSELMLVNDCSFNFGLVKWTRPSQEKNEFDIKVKENGTYA
                     FYVFVNGKVKGYVRFKIKGFSKNAEGTGVTQYWGQGAKYYELPEEHVNPEDFRDKDGEN
                     NGICVNEKIPDDKPGGSGDIEEPKEDNKPPVEGGGSDKAIKEALDKITSAVNEISKNTG
                     ETAKNTGEIKKNTDEIAKNTKEIADAVKDIRDNLKITDDMDLSIKPVLPEDIKPDKPKQ
                     PDKPFEDKQEHFKEGEQEKPPDKLPVAPEPAECWGDVCKDKDNQKEEDMKKDKDMDKEE
                     PMTPDKPMEKEDMKPDKPMTPDKPMEKEDMKPDKPMTPDKPMEKEDMKPDKPMTPDKPL
                     EKENMKPDKPMTPDKPMDREGEMKKDNDMKRDKPMTPWEEMKPSDEGSELCWHVVNGKC
                     G"
                     /name="hypothetical protein"
     gene            complement(8219..8377)
                     /locus_tag="PJW02_RS27665"
                     /old_locus_tag="PJW02_27665"
     CDS             complement(8219..8377)
                     /locus_tag="PJW02_RS27665"
                     /old_locus_tag="PJW02_27665"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000425805.1"
                     /translation="MEMPKISLTYNLADVANGLTNWLGAYWPIIAFSVAIPLTFLVAFN
                     TKDLFTR"
                     /name="hypothetical protein"
     gene            complement(8398..8550)
                     /locus_tag="PJW02_RS27670"
                     /old_locus_tag="PJW02_27670"
     CDS             complement(8398..8550)
                     /locus_tag="PJW02_RS27670"
                     /old_locus_tag="PJW02_27670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001113828.1"
                     /translation="MPEFGFRPSMSQLFGNFHMNWSTMMPLLVILFGILFGFFVAHLVK
                     DKFDD"
                     /name="hypothetical protein"
     gene            complement(9144..9449)
                     /locus_tag="PJW02_RS27675"
                     /old_locus_tag="PJW02_27675"
     CDS             complement(9144..9449)
                     /locus_tag="PJW02_RS27675"
                     /old_locus_tag="PJW02_27675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001176491.1"
                     /translation="MQLMKVMLTGCKKVDFTNDNNERIEGVNIHFLTTTDNSEQSGFGY
                     LPSKVFFRKEFIEKFSSLNYPIVAEAIIAFNMSPRGIQTKISDFNILEEFPFTALK"
                     /name="hypothetical protein"
     gene            complement(9610..10680)
                     /locus_tag="PJW02_RS27680"
                     /old_locus_tag="PJW02_27680"
     CDS             complement(9610..10680)
                     /locus_tag="PJW02_RS27680"
                     /old_locus_tag="PJW02_27680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410863.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="WP_176887198.1"
                     /translation="MEKHIEKLSLSVCVDWLEFTFVYGEEFESICSFLGLDSTVFSKEI
                     DGFHKSYGYLSRYSFEEIHILMHGADNRSRIIMSGSGCRWFETLSSVGWYGLFDRIAFA
                     DEHGFSWIKVDRLDIAIDDFVGYFSVKKLKSKIKRRECLSRWKTSFVVETFDLEQGRLQ
                     GSTIYFGSASSKLRWVVYDKLEEQKKKNKDSDSIQGLEFWTRHELRLKKERADRAVKQL
                     VVNDFSIEEMYFGIINNYISFRVVDKEDSNRNRWNIAPFWRQYIGQASKLRLAEALPVA
                     NVERTFKWLSHSVSKKLYLLSEIFSQDDDTKKEFASYLENKGKQKITKGDLDLIENFPI
                     RDFKEFLKIYNEKCLK"
                     /name="replication initiation factor domain-containing
                     protein"
     gene            complement(10868..11059)
                     /locus_tag="PJW02_RS27685"
                     /old_locus_tag="PJW02_27685"
     CDS             complement(10868..11059)
                     /locus_tag="PJW02_RS27685"
                     /old_locus_tag="PJW02_27685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000048809.1"
                     /translation="MSKVKGIRLNLRVSEDEYNKLQYVKECLLMEGLDLKDSAVIRYAL
                     LQTYKDYKEMNDLLKSRN"
                     /name="hypothetical protein"
     gene            complement(11386..11664)
                     /locus_tag="PJW02_RS27690"
                     /old_locus_tag="PJW02_27690"
     CDS             complement(11386..11664)
                     /locus_tag="PJW02_RS27690"
                     /old_locus_tag="PJW02_27690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021729234.1"
                     /translation="MAYSYCVDCESVYNCEDEKCCCGSKKRSVDWEEFAFIMGYPLYPV
                     KKKYGVFHLDLSKRLDADALCDLKADNPELFYYEFKGWKERELKKRN"
                     /name="hypothetical protein"
     gene            complement(11678..11899)
                     /locus_tag="PJW02_RS27695"
                     /old_locus_tag="PJW02_27695"
     CDS             complement(11678..11899)
                     /locus_tag="PJW02_RS27695"
                     /old_locus_tag="PJW02_27695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000391859.1"
                     /translation="MEELLHKIIDKLDKIDRDIQEIKTDLKAHRIETDDNFIKLYHLVE
                     EKDVEIDVLNKRLFKNEATVERIKREKH"
                     /name="hypothetical protein"
     gene            complement(11979..12194)
                     /locus_tag="PJW02_RS27700"
                     /old_locus_tag="PJW02_27700"
     CDS             complement(11979..12194)
                     /locus_tag="PJW02_RS27700"
                     /old_locus_tag="PJW02_27700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001169628.1"
                     /translation="MQKISEERKHRSFRFTDSELSLIDELVDIENDRRMTIAREMNLKY
                     KALNRTSFILYMAEERRRKYEEQGEI"
                     /name="hypothetical protein"
     Typing          complement(9611..10680)
                     /name="rep_cluster_814"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.907"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(9614..10680)
                     /name="MOBT"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.719"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaacgaaat gacctatgtt ttctttcttc gcttattttt tgcataacta aaacctccta
       61 taaaactatc gtatacacaa tataacacat taaaattaca aaaacaacct ttgtatacac
      121 aaaaaaaata aaagtgtaca caaaggcatg tggtcaaatc atatttcaaa cttttttaat
      181 acatttcaaa acaatatttt taaaataaac tctaacaaaa taccaaatta acaatatcaa
      241 aaatcctgtc gtaaaaataa acaaatcact atatatatca gatacatact ttgttgtaac
      301 aacacagatc atcgcaagca tacatatatc aaaaactaca gttaaccaaa atttaaaatt
      361 caactttaaa tcagcaaaca tataatcaac atccccacaa ttaaaattac aagaaatgat
      421 aaaccagctc tatttgctat tttttcacca tacttattac ataaataagc atgcaactta
      481 tcatgttgtt tagaaagaaa tctataaaaa tactttgtta ataaaagtac aaaaagataa
      541 tagattccaa ttaaaataac aaaaagaaca aataacgtta acatattaac ttcaggtgaa
      601 atacaaccaa atttaattag aaatgtcata atagcaccta aagccaaaaa ttgaagttgt
      661 gacataaact catcatcttg actacgatat ctagcaaaca tacatacacc tcttaaactt
      721 cactattctt taaactaaat ttactcatta atctaagagt caacataaga aaactaacat
      781 taagaataaa agcaaataaa agcgatatta cacttaaatc cttaaaatat accgatattc
      841 ctaaatacaa aagaaacaat aaaaaatttg taaaaactgc atatccaata ttttcaccac
      901 ttatagcata tttcctaaaa atcatctttt tatcttttaa tttatctata ttttcattaa
      961 caaatttctt atccatagat acatacttaa caaaaataaa aagggcaatt ccaattgcta
     1021 ttagagttaa taaattaaat ttagcaccaa ctaaacacca actaaaataa aaaaacatcg
     1081 caacaatatt aaacaataca tgaatatatt cacgataaaa cttttcaaac atatatatcc
     1141 cccttaaaac gccttatttc gcttcacaac ctcttttttt ataaactaca tagatatgac
     1201 tatctatata acttaaatcg tcaaatatcc aacctctgtc ttccaaatac tccttatgta
     1261 ccttaacttg gttagattga tcacttctca aatatttcaa cgttttagat aaaacagtta
     1321 cctctttttc attacaaata ggcaaatcca ttacatatca cccttctttt aacgtgtcta
     1381 acatgccttt aaacaagaaa caaatcccta gagtaccaaa caacgtaaca ataacattct
     1441 ccattatcaa tcaccaaaac cacagataga acccgaatca tacgaagaac tagaacctga
     1501 atcgtaagat gaactagaac aaccagaatc ataagatgaa ctagaatcat atgaactata
     1561 tgaagatgct gctaatgatg tagcaattaa ataatccgaa tttgaactac ttgaactaga
     1621 tgtttttttg ctacttgaat cattcgattt cttattacct acattagaat aactactttt
     1681 atttaagtta actgttggag aataatcata accttttctt gaagaactat atgaatatcc
     1741 atctccaata aacaacttct taatagctct aatcataata gcaacaacaa tacatcctaa
     1801 aattaacgct atattaaaaa taacagtact aaatatattt tgtaattgac caatataaat
     1861 ttgttccata ttaatttccc ctttctttta aaacctacct ttttgaaaaa aggtaacaaa
     1921 aactttccaa cgtgggaggt cccacacccc attcgcctac agcgggtgtc ccccaaacaa
     1981 tgtttgggag aacgcaatag cttgtttttc tattccttta ggcaaccttt caggacatca
     2041 acttttataa gtaaaacttt ttacaaacca aaaaactttt tcttataaaa ctccttcgct
     2101 ttgctacggc ttcttacaca ctttccattt ccatctatcg attcacattt caagcgcttc
     2161 agaagtgatt ttgcgtgttc tgatgcttat ttacagcatt ctctagttct atttaacctg
     2221 caaaccaatt taatagaata aaaatcacag caataaatga accgtaaaac aatccatctc
     2281 tcgccatact tttcaaccaa tctacatgta tcatttcaac tatttatcct ctctaataaa
     2341 tatccatatc actaacaaat aaataagcaa tcacaatcga acctaaccac aacatactag
     2401 caaatacaat acattcaacc attccatcac ctattaaata tttttttctc gatataaatc
     2461 aaaaaagcaa taaacataat actctctaca aatacaaccg tttcacgagg atatttagtt
     2521 acaaaatcaa aaactatcat ctacttagcc atttgactaa acatcacatc taaattatca
     2581 aaatgatgtt tgattacttc acaattattt atttcttctt cgtctaatcc atagcgatcc
     2641 ataagcaaat ctagaaattc tctcatttct ccatgtgctt tatttattct tttcacttct
     2701 tcacgaaatc ttttcttctt taaaatcccc ataacaacgt tccttcctta atataatttt
     2761 tataatctta tgaagtcctc ccgcctgtac cgcgcataca gcttacgctg aatgcttgta
     2821 ctggcgtccg tactcttcgg cggtcgtctt caacgcttct aaaaattcca taaagtcttg
     2881 gcgtttttct ggaactttta ctggcgaaac cattgcactt gtatcataaa tttttgaacc
     2941 aacaacaaaa gctttctttt tacttatacg cattctcttt aaataacgtt tagattgaat
     3001 gtcatacatc gtatagtaaa aatacttttt atcacttgaa accttaacaa gaacgttcgt
     3061 aatacctcgt atacgtgcat ctaaatcatc aaaactaggc gaagcaataa acaacgtaca
     3121 acgtaatttt cgaagataat acgatacttg agaaaagaat ttaaccgaat tactcgaaaa
     3181 gctccgagca tctaaatcaa tgtgagcttc atctaaattt aaaattgtag atttttcttg
     3241 agcaatattt ttaaaatgtt ctatttcagt aaatggcttt gaaccaacta cgccataatt
     3301 gctatacaat acacaattac ttttctgttt aaagtggtgt gcaaacaatg tcattcctaa
     3361 tgtcttaccg cttccaagag gaccttcaaa gaccattacg ttcatgcaag ttcatttcct
     3421 ttcactactt ttgcactatt agctgaaagt gataatgatg gattcgcatt cgctttcagt
     3481 aagtgactta atttcgcatt acctttaatt ctgtcaaaca tagttaatat ctttagaacc
     3541 ttttcataga aggcaagtgg taaactctgt tttttcacag agataacata atcagctata
     3601 tctttacatc cattctcatt aagcaataag atacctttca tttgagatgc attcaaaggc
     3661 actgaatttt ccttcatgta ttgaaataat tgaaccgttt caatactttc acttgaagta
     3721 tcttcttcta aaatctcttc tttcattatg tcttgaacat ctggtaacac ttcttctaca
     3781 acagtttttt ctaaagtcac ttcactcatt tagttcaccc cattatttaa aaatagctag
     3841 taaaacaata attacccatg gcataaaacc aattaaatca aattgttttt ttcctgtttc
     3901 aaattcaaaa atccctttta acgcactaga tcgtcgtaat cttttaatat tttccgcttc
     3961 aattcttgcc ggtttttcta agttgtaaat gtgatacaca taaccgtgat tatcatcaag
     4021 taatctcaat tcattctcac gtggtaacat ggctgtactc gtttctaaaa tctccgaatc
     4081 aaacgagata acctgttcaa ctacacatgt accctcttcc ttaataatta acgctctatg
     4141 tccttcaaat tgtggctgta ctttccgttt cgttttagct aacatttcaa tcactctctc
     4201 tttttaattc cattttcttt ttaaccacat gcaaggtaac actataaata gaaatacaga
     4261 taaacaaatc gcacttgata actcaaacat tgcattaaaa gcctgcattt ttaactctcc
     4321 tttttcgaag ttgcatcaca aactaaaatt aataaacaac taacgactcc caatattaaa
     4381 taaatcattc ttctatccct ctaaagaatt tcaatgtatc tattaccatc caagaaacaa
     4441 ctgttgtaac aaatattaat aaaattgctt gccctacaat attcaatatc attaccacca
     4501 tttcactgta tctttaaatc gaataaataa actactgtaa gtcatgatga tgtaacaaac
     4561 aaaatatacg aaaactaaag caaataacgt cgttataacg gttgtccaac cgtataaata
     4621 agcaaacact ccaaggtaat cactaattcg caaaggacga cttgctggta cgcttaaatc
     4681 atttaaatac tttgccattt gtgcaagcca accaagaaca ggattaaata ttgtatcaat
     4741 taacgcattc attaatgatg accacctctg aatagatcca tgagcttttt aacaaacaaa
     4801 taccaaataa aacatgtgac aaccatcgga actactgtta acaaaccagt aggaagtaat
     4861 atatctgtcg ctgtcttaaa tcccttcatc gaagcactag gaaaagatac attcccacca
     4921 tcaaaattag gttgtaccca tagaaataaa gttttaaaga atccagctat tacagcacct
     4981 aaaaactgaa acaaggcaac gaataatttt ataattgcta ctacaacttg aaacagtttt
     5041 tctatgaaat aaaagattcc ttttaaaaaa tccagtagat attgaaatgg tgtcgctaaa
     5101 aaatcaataa acgtttttaa cgactttata accgcttcaa atccgccttt taagaactta
     5161 acaaacttct ccatcatgat tgcttataac ccaccataat ccttctaatg cttacagcaa
     5221 cagagaatgt aatcattaat acaaacgcta gaaataatac gtcttcccca gcatggaacg
     5281 catactggag aaattgtatt aagtccttag ttgtaactgg ttgtatcaaa ctaagcacct
     5341 acctcataaa caactttttc gtattaaacg caacaataaa cgctaacgga atagcaatac
     5401 taaatgccac aatcggccat atccccaaga accaatttgt tatcccatca actaaagcac
     5461 ctaaaccaaa actaaaagta acctcaggaa taactggtac tttaggaatt ccattaacaa
     5521 cattgcttaa atcactttca atattccact tagaaaccgc cttaatctgt acaccataag
     5581 tttgaccatt ctgtaaacct ttcacagcat aagaacgact tgtaacaagt gaatcattaa
     5641 cttttacacc atcaataaaa atgtgataac ccttaattgg aacgcctaaa ttttgactta
     5701 atagatttac aattaacgag ccatcttttg gcgtaatatc aaaattcgaa ggtttagaag
     5761 gcttattgag attaggttct tcatcagtct gtactttctt cacaattcca tttgattcaa
     5821 gaccagtaga ggaaactgaa acaaatttat aagtatatac agtacttcct tttagacctt
     5881 tatcaacaaa ttcctcaatc ttcacattat ccgcaatcac cttatcatca cgataaattt
     5941 tcacatgatc aaaattattt ttaggaaact tataactaaa tttcacataa gtatgagtta
     6001 tttcatcttc ctgtaaatct tttatttcat ctggtaaagg ctctttcgct gtctttatct
     6061 ttttctctat accttttgat tcaagaccat caggagaaac tgaaacaaat ttataagtat
     6121 gctccgtttc aggagttaaa cctttatcag taaatttttc ttctttcaca tcactagcaa
     6181 gcacttgtcc atcacgataa attctcaaat aactaaaatt cgatttcgga tttttataag
     6241 taaattcaac tgaatcatgt ttaatatttt cttttaaatc cgcaatatca gatggaggaa
     6301 cagattcata ctcatataaa ttaacttcag aaattgtaaa ataatctcca ctacttgtat
     6361 tttccaatac aacttttgta acacccttaa tatcaatagg atttacataa ccactatcag
     6421 ttttgctata tcctttcttt aaaactcgac cctcagtatc ataaaaataa acattcatat
     6481 agttactaga accaccaact ttagtaaact taaaccctga aactctaaca ggagactgta
     6541 aagtaaatgt aatttctccg ttagctaaag tatcactagt ccttaaattt ccatcagtta
     6601 acgtagatac aattgtgcca aatctatcct taccatacct tccacttaat acatctttac
     6661 cttgagcaaa tgcacttgta tcacctacaa acaaaatccc aagtaataca ggtactacta
     6721 taaaaaattt ctttatccac attttccatt cactacatgc caacaaagtt cactcccctc
     6781 gtcacttggt ttcatttctt cccaaggagt cattggctta tctcgtttca tatcattatc
     6841 ttttttcatt tctccctccc gatccatagg cttatcagga gtcattggtt tatcaggttt
     6901 catattttcc ttttccaatg gtttgtcagg agtcattggt ttatcaggct tcatgtcttc
     6961 cttttccatt ggtttatcag gagtcattgg tttatcaggc ttcatgtctt ccttttccat
     7021 tggtttatca ggagtcattg gcttatcagg tttcatgtct tccttttcca ttggtttatc
     7081 aggagtcatt ggttcttctt tatccatatc tttatctttc ttcatatctt cttctttttg
     7141 attgtcttta tctttacaaa catcgcccca acattcagcg ggctcaggag caacaggaag
     7201 tttgtcaggt ggtttctctt gttcaccctc tttaaagtgc tcttgtttgt cttcaaaagg
     7261 cttatcaggt tgtttaggtt tatcaggctt aatgtcctca ggaagaacag gtttaatcga
     7321 caaatccata tcatcagtaa tcttcaaatt atccctaata tccttaacag catccgcaat
     7381 ttcttttgtg ttctttgcta tttcatcggt gttcttttta atctcacctg tatttttagc
     7441 tgtttcaccc gtattctttg aaatttcatt tactgcactt gtaattttat ctaaagcttc
     7501 tttaatagct ttatcagaac caccaccttc aactggtggt ttattatcct cttttggttc
     7561 ttctatatct cctgaaccac ctggtttatc atcaggaatt ttttcattca cgcaaattcc
     7621 attattttct ccatctttgt ctcgaaaatc ctcaggattt acatgttctt caggaagttc
     7681 ataatacttt gcaccttgcc cccaatattg agttactcca gtaccttcag catttttaga
     7741 aaatccttta attttaaaac gaacatatcc tttaactttc ccattcacaa acacgtaaaa
     7801 agcatatgtt ccattttctt tgactttaat atcaaactca tttttctctt gtgacggcct
     7861 agtccatttc actagtccaa aattaaaact acaatcatta acgagcatca actcactcaa
     7921 ttgaaacgtc actacgtctt tagcatcact aatctttaac gaaagactgt aataaccgcc
     7981 aacaagtcct ttattttcta tggaaacccc tttacttcct accatttgaa atgatggcgt
     8041 atcagcaaaa acaggaactg tacggaagat aatacaaaat gtacataaaa aaattatggc
     8101 tgcttttttc atatcaatcc ttcctaacaa aaaatagcta ggcgatccac ccagctattt
     8161 gattgtttta ttaaatttat agttaacttc ttgctattta aggagtgatt tttgatgatt
     8221 aacgagtaaa taaatcttta gtgttaaacg ctactaagaa cgttaacgga atagcaacag
     8281 agaatgcgat aataggccag taagcgccta accagttagt taaaccattt gcaacatctg
     8341 caagattata agttaatgaa atttttggca tttccaaaat aaattcctcc tagttaatca
     8401 atcatcaaat ttgtctttta ctaaatgcgc tacgaaaaat ccgaacagta tcccaaacaa
     8461 aatgactaac aatggcatca ttgttgacca attcatatga aagttaccga ataattgaga
     8521 catcgatggt ctgaatccaa attcaggcat tgaaatcaac tcctaaaggt gttgtacact
     8581 ttaccaatac agtagatagc aaacgcccca ccaatgaata aatagatcac tggtaacact
     8641 gaactaaaca tgttgaaggc atactgcaag attcttccca cgtccatgct aaaactaata
     8701 gccatcaccc caagctttga cggtggtgaa catcattatt acaaacacaa gaaaggttat
     8761 aaaacctaac gaatataata caactgaatt attcatgaag aaccaataca acatatctag
     8821 aaacgctaaa tcattcatta tctatcaaac cgccaatctt tcagcaatgt gaacacgata
     8881 attaataaaa atggtaatcc aaacaaggaa tattgattca acaagttttc taaattaaac
     8941 tcgatcatcg taaagcactc catatccagt tgaatgtcaa caccaacaag attagcaata
     9001 acaagaaact aattaacata tcaccatagg taagtgtctg gaaaactgca aaactaccat
     9061 tcttagtctg cactaagtaa ttaggcataa tttccgaaat agttttgtac aattcattta
     9121 aatcgatttt atctatcata aaatcacttt aaagctgtaa aaggaaattc ctctaaaata
     9181 ttaaaatcag aaattttagt ttgaatacca cgaggagaca tattaaaagc aataatagct
     9241 tcagcaacaa ttggataatt caaagaacta aacttttcta taaactcttt tctaaaaaag
     9301 accttagaag gaagatatcc aaaaccagat tgctcactat tatcagttgt agttaaaaag
     9361 tgaatattaa caccttcgat tctttcatta ttatcatttg tgaaatcaac tttcttacaa
     9421 cctgttaaca ttactttcat taattgcata tttacactcc ttagaatctt tttagtcata
     9481 caatgtcgtt ttatgtacac ctttgtcata catttaatta taagaaaaac tagaaaaaaa
     9541 ttctagtctt tttttaaaaa tatatggatt ttttaatgta ttagacttcc attataaaat
     9601 agagtaaaat tattttagac atttttcatt ataaatcttt aaaaattctt tgaagtcacg
     9661 aattgggaaa ttttctatta agtctaaatc ccctttcgtg attttttgtt tgcccttatt
     9721 ttctaagtaa ctagcaaact ctttctttgt atcatcatct tgcgaaaaaa tttcagacaa
     9781 caaatataat tttttagaaa cactatgaga caaccattta aacgtacgct caacatttgc
     9841 aacaggtaaa gcctctgcta atcttaattt acttgcttgt cctatatatt gcctccaaaa
     9901 aggtgcaata ttccacctat ttctattaga atcttcctta tcgacaacac gaaacgaaat
     9961 ataattattt ataataccaa aatacatttc ttcaatacta aaatcattaa ctacaagttg
    10021 tttaacagca cgatcagcac gctccttttt caaacgcaac tcatgcctag tccaaaattc
    10081 taaaccttga atcgaatcac tatctttatt cttttttttc tgctcttcta acttatcata
    10141 aacaacccat ctcaatttac tagaagcact tccaaaataa atcgtagaac cctgcaatcg
    10201 tccctgttct aaatcaaaag tttcaacaac aaaacttgtt ttccaacgac tcaaacattc
    10261 tcttcttttt attttagact ttaatttttt aacagaaaaa taacctacaa aatcatcaat
    10321 agcaatatct aaacggtcaa ctttaatcca agaaaaacca tgctcgtctg caaatgcaat
    10381 cctatcaaac aaaccatacc agccaacaga ggataatgtt tcaaaccatc tgcaaccaga
    10441 accagacata ataattctag aacgattatc agcaccatgc ataagaatgt gtatttcttc
    10501 aaaagaatat ctagacaaat aaccataaga cttgtgaaaa ccatctatct ccttagaaaa
    10561 aacagtagaa tctagaccta aaaaactaca aatagattca aactcttcac cataaacaaa
    10621 agtaaactcc aaccagtcca cacaaaccga aagagacaat ttttcaatat gtttttccat
    10681 acaaacacct ctaaaacaat acaataaaag caaaataaaa accgtgaatt ttttgcctat
    10741 ttttttaaaa gaaccgtgaa aaaaacaaag aaggacgaac cttagagcca caagcgtttg
    10801 agggcggttg acctgtattt tctactgggg tgttactagc ccccagtaga aacatgaaat
    10861 cagccattca attccttgat ttcaacaaat cattcatttc tttataatcc ttataagtct
    10921 gtaataaagc ataacgaata acagctgaat ctttaagatc aagaccttcc ataagtaaac
    10981 attctttaac atactgcaac ttgttatact catcttcaga aactcttaaa ttcaaacgaa
    11041 tacctttaac ttttgacatt atataacctc ctcaattgaa gcatacaatg tcatacacca
    11101 tatgtaaaga gtttccattt tcctccttgt aaatattata ccaatatata aatcttttaa
    11161 ccttcattaa aaaatggaaa ttttatcaat gattttccgt gcattttttt gtgaattctg
    11221 tacagattta aaagtgactt gaacccagtc cagggaaggg attgtgtaga tatcgaaaca
    11281 gccgggaacc cccgtgttac tggacgggag ttcctcattt cgaaactgga aattttcctt
    11341 cgaattagac aaacaaaaca cccactaact ccaaaatatt tattattaat ttctcttttt
    11401 caattctctt tctttccaac ctttaaactc ataataaaaa agttctggat tatctgcttt
    11461 aagatcacat agagcatcag catctaaacg tttagataaa tctaaatgaa aaacaccgta
    11521 ttttttctta acaggatata acggataacc cataataaaa gcaaactctt cccaatcaac
    11581 agaacgtttt ttacttccac aacaacactt ttcatcttca cagttataca cactctcaca
    11641 atccacacaa taactatatg ccatcgacaa tttcctccta atgcttttcc ctcttaatac
    11701 gttcaactgt cgcctcattc ttaaatagac gtttattcaa tacatcaatc tcaacatctt
    11761 tttcctcaac taaatgatat aacttaataa aattatcatc agtttcaata cgatgagcct
    11821 tcaaatctgt tttaatctcc tgaatatccc tatcaatctt atctaattta tcaataattt
    11881 tatgtaacaa ttcttccata attaaaacct cccaaattta atcttaaaaa agtgcgactc
    11941 tatccctatt attattaact agttaagtag agaactattt aaatctcccc ttgttcttca
    12001 tatttacgtc ttctttcttc agccatatat aaaataaaac ttgtacgatt taatgcttta
    12061 tattttaaat tcatttctct tgcaattgtc attcgtctat cattttcaat atcaactaat
    12121 tcatcaatta atgacaactc actatcagta aaacgaaatg acctatgttt tctttcttcg
    12181 cttatttttt gcataactaa aacctcctat aaaactatcg tatacacaat ataacacatt
    12241 aaaattacaa aaacaacctt tgtatacaca aaaaaa
//
