LOCUS       NZ_CP116255             2894 bp    DNA     circular CON 27-FEB-2024
DEFINITION  Enterobacter roggenkampii strain 120063 plasmid p5_120063, complete
            sequence.
ACCESSION   NZ_CP116255
VERSION     NZ_CP116255.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19316169
            Assembly: GCF_018596855.2
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Wei,L., Wang,C., Liu,L. and Zong,Z.
  TITLE     The complete genome of Enterobacter roggenkampii strain 120063
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Wei,L., Wang,C., Liu,L. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2023) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 2894)
  AUTHORS   Wei,L., Wang,C., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2021) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. v0.5.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina Hiseq-X10; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/27/2024 02:58:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,285
            CDSs (total)                      :: 5,165
            Genes (coding)                    :: 4,946
            CDSs (with protein)               :: 4,946
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 38 of 219
            Pseudo Genes (incomplete)         :: 148 of 219
            Pseudo Genes (internal stop)      :: 57 of 219
            Pseudo Genes (multiple problems)  :: 23 of 219
            Pseudo Genes (short protein)      :: 1 of 219
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116255.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="120063"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1812935"
                     /plasmid="p5_120063"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2019-05"
     gene            complement(407..703)
                     /locus_tag="KK030_RS26220"
                     /old_locus_tag="KK030_26165"
     CDS             complement(407..703)
                     /locus_tag="KK030_RS26220"
                     /old_locus_tag="KK030_26165"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_077951050.1"
                     /translation="MMASNLIWEEEAIDDRADYFEWLYQQNPKAAEDADQYIEDTVDLL
                     IENPFLGKDSEIVSGVRVLIIKGLSLNVYYQEGHGTVKVLRVLHQSRNIAKLI"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(700..1104)
                     /locus_tag="KK030_RS26225"
                     /old_locus_tag="KK030_26170"
     CDS             complement(700..1104)
                     /locus_tag="KK030_RS26225"
                     /old_locus_tag="KK030_26170"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_059363301.1"
                     /translation="MGFISQLSKTKISTRRGSASQITVTQSFKPSTSAEELSIRISTEC
                     LNNISLKIGDKVDVLYDADSDTWMIKKEAEGYKISGKPDAPTGLIRYTLKEGHKKFTST
                     RESLPVKKDSNDDTIVFMGDKMTFKLKESE"
                     /name="hypothetical protein"
     gene            1294..1578
                     /locus_tag="KK030_RS26230"
                     /old_locus_tag="KK030_26175"
     CDS             1294..1578
                     /locus_tag="KK030_RS26230"
                     /old_locus_tag="KK030_26175"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="WP_059363302.1"
                     /translation="MDTKFHFRLDENVKMRAQKAAEARGKTLSEACRDFAEQLAREVEP
                     LSQHEKWLKKQVELAVAKANSGDGRFTSNEEVKRVMASRKDEIRAKYKR"
                     /name="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     gene            2460..2660
                     /locus_tag="KK030_RS26235"
                     /old_locus_tag="KK030_26180"
     CDS             2460..2660
                     /locus_tag="KK030_RS26235"
                     /old_locus_tag="KK030_26180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_059363303.1"
                     /translation="MADDRTLQLRLTGFRKAEASLRLEGMDPSGTPLYESVKARLLSGE
                     ITYDEGRAEILAHHQKRANSN"
                     /name="antitoxin VbhA family protein"
     Typing          complement(1768..2417)
                     /name="rep_cluster_2324"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.464"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgctcaccgt tatctagaga gttctgtgct ggaaatgagc atctggttac aacatccgtg
       61 cgaaactatc ctccattcac ttccgtgcat agccatgcag acgcgcagaa atcatcaggt
      121 gcgtttttca gggttatggc caggaaaggt gctggaaatg gctaactcgt tgcagagtga
      181 cgctgagggc ctttcagagg gtgcgcgtaa gaagggatta tggtaaatac gtatatccta
      241 aggaaaaagg tctctacttg aatgagcttt ttccttagga tttgagctgt ttgcctaatt
      301 tataagggcg tagactggct ctgtcttctg tctagaaaac aaaatacatt gttaaaacat
      361 tttttgagtt gcttctttct ccctcgctga ggcaggttgc gttgccttaa atgagttttg
      421 ctatgtttcg tgattgatgg agaactcgta gaacttttac tgttccatgg ccttcttgat
      481 agtacacatt taacgatagg cccttgatga tgagaactct cacgccagac actatttcac
      541 tatctttgcc gagaaatgga ttctctatta gtaagtcaac tgtgtcttct atgtactgat
      601 ctgcatcttc agcagctttt ggattctgct gataaagcca ttcgaagtag tcagcccgat
      661 catcaatagc ttcttcttcc caaatgagat tgcttgccat cattcgcttt ccttcagctt
      721 aaacgtcatc ttgtcaccca tgaatacgat ggtgtcgtca ttactatctt ttttaaccgg
      781 aagtgattcc cgggtactgg taaatttctt gtggccttct ttcagtgtgt agcggatcag
      841 tcctgtcgga gcatccggtt ttccggatat tttatagcct tccgcctctt tttttatcat
      901 ccatgtatcg ctatctgcat cataaagtac gtcaacctta tctccgattt tcaagcttat
      961 gttgttgagg cattcagtcg agatcctgat ggaaagctct tctgctgacg ttgaaggctt
     1021 gaatgattgg gtcactgtta tctgactagc agatccccgg cgtgttgata tttttgtttt
     1081 tgaaagctgg cttatgaatc ccatacatcc tccttttttc tcttgtttgt attttaactg
     1141 atcttcactg atcgatcatc atccttcttc gtttttttct atccgacatg atcattgtat
     1201 ataagtcaat gtttttagtg ggtttttatt atataaatat gtgtgttgct tgtgcgcaca
     1261 actgtactaa cattacgggt gtcggaggat cttatggata ctaaatttca tttcaggctt
     1321 gatgagaacg tcaagatgag agcccagaaa gcggcagaag cccgcggcaa aactctaagt
     1381 gaagcttgca gagattttgc tgagcaattg gcaagagagg ttgagcctct tagtcagcat
     1441 gaaaaatggc tgaagaagca ggtagagctg gctgttgcaa aagctaattc tggagacggt
     1501 agattcactt ccaatgagga agtgaagcga gttatggctt caagaaaaga cgagataaga
     1561 gctaagtaca agcgctagcc tgtatattga cgctaatact gaatatgcgt aatctcgttg
     1621 aaaagaaaaa accgcctggg gaggcggttt tttcaaggtt cagtaagttg gggaacttct
     1681 gaaccgtggt gactggctta aacggagcgc agcgaccaaa tctgtccttc cagtgtagct
     1741 gtagttaggc taactcttca agaactctat gtgtacttat atacactgct ccgctccgta
     1801 aacctgtcac caatggctac cgccagtggc gattcgtcgt gcctttcagg gttggattca
     1861 agagtacagt tacctgaaag tcgtggtagt cgggttgaac ggggagttcg tgcatacagc
     1921 ccagcttgga gcgaaccacc taaaaggaac ccagttgcac gggtaggaat gagaaaacgc
     1981 caccttacgg cggtctgcaa cgggtaaacc tcagggtggg aacaggagag cgcacgaggg
     2041 agcgacccat cggaaacggt ggggatcttt aagtcctgtc gggtttcgcc ccttctgact
     2101 tgagcaccgg aaatcgccag atttctgtga tgttcgtcag gagggcgaag cctatggaaa
     2161 accggcaatc ctacagacct tttacatggc tttctgcctg agctggtaac gctcgccgca
     2221 gcgtcatgac cgaacgaagt gagtgaatga ccgaggaagc gtaatgtctg taatgctgaa
     2281 cagggctcgt atcgctcggt acaccacttt cctctctgat tacacactct ggtgcaacgc
     2341 tttcctgccg ttttccactt ttccctgcac agctgtacaa cggcaacatc cgggcaggat
     2401 atgtaaagtg ggcccacttt acgcctgcct tattcgcatg actgctcaaa ggaaaaccca
     2461 tggctgatga tcggacactg caactgcgcc tgaccgggtt ccgcaaggct gaagcatctc
     2521 tcaggcttga aggcatggat ccttccggaa cgccgctcta tgagtccgta aaagcgcggc
     2581 ttctttccgg tgagatcacc tacgacgaag gccgcgctga gatactggcc catcaccaga
     2641 agcgggcaaa cagtaactga cgcggacagg aaatgttttg tgaccacagc cagctgcgcc
     2701 ggcgccacaa aacagcggga cacgatgtgt ttttggagta ccgccggcac gccaaatcct
     2761 cctggtcagg ctccgcccga cacccgccag gaactacggt cccactcctc gttaaatccg
     2821 acccctttgg cgatattcct gccgcgcctg gcggcttgcc caacgcctca cggtcgcaca
     2881 gtaaactgcg cacc
//
