LOCUS       NZ_CP116253             3062 bp    DNA     circular CON 27-FEB-2024
DEFINITION  Enterobacter roggenkampii strain 120063 plasmid p3_120063, complete
            sequence.
ACCESSION   NZ_CP116253
VERSION     NZ_CP116253.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19316169
            Assembly: GCF_018596855.2
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Wei,L., Wang,C., Liu,L. and Zong,Z.
  TITLE     The complete genome of Enterobacter roggenkampii strain 120063
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Wei,L., Wang,C., Liu,L. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2023) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 3062)
  AUTHORS   Wei,L., Wang,C., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2021) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. v0.5.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina Hiseq-X10; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/27/2024 02:58:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,285
            CDSs (total)                      :: 5,165
            Genes (coding)                    :: 4,946
            CDSs (with protein)               :: 4,946
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 38 of 219
            Pseudo Genes (incomplete)         :: 148 of 219
            Pseudo Genes (internal stop)      :: 57 of 219
            Pseudo Genes (multiple problems)  :: 23 of 219
            Pseudo Genes (short protein)      :: 1 of 219
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116253.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="120063"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1812935"
                     /plasmid="p3_120063"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2019-05"
     gene            1..132
                     /locus_tag="KK030_RS26175"
                     /old_locus_tag="KK030_26120"
     CDS             1..132
                     /locus_tag="KK030_RS26175"
                     /old_locus_tag="KK030_26120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032159035.1"
                     /translation="MHEPPVQSDCCAFSGNYQLESNPERHDKAPLAAAIGNWISRAG"
                     /name="hypothetical protein"
     gene            467..1138
                     /locus_tag="KK030_RS26180"
                     /old_locus_tag="KK030_26125"
     CDS             467..1138
                     /locus_tag="KK030_RS26180"
                     /old_locus_tag="KK030_26125"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_048230210.1"
                     /translation="MNKSNFRALTDEEVANCVKLIRHDRKWSQEQLAAISGLNLRTIQR
                     VEKGEPSSIDTRRNLANSFGANDIDAFNKETYIPSMDELKKYQDYIEDSCEQIKATKLN
                     TSIGLLDATEGCHASFFDSSFDMSHDAEEANHLIKCYLKEYMDCHDLYNKEQKNDAAKE
                     IQVHIDKLNKLGVALVYAKKKTNITNIGNNISMETIYIIAFPIGKEPDTLIIPNEADVS
                     V"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(1223..1627)
                     /locus_tag="KK030_RS26185"
                     /old_locus_tag="KK030_26130"
     CDS             complement(1223..1627)
                     /locus_tag="KK030_RS26185"
                     /old_locus_tag="KK030_26130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="WP_008321396.1"
                     /translation="MTILVTIQAQLVVGEEQVIKSLAPEGMLAAVFEDDGRTGYFYALD
                     ESVEGNPIMDAVHIYNAEDVSDGHIPSDVKIGWSEDSQKCVLLINGYPHGAFDFVGKNG
                     YCRSGYPPPINKVWSLSGHEWSDSVDDFFR"
                     /name="DUF2251 domain-containing protein"
     gene            1887..2075
                     /locus_tag="KK030_RS26190"
                     /old_locus_tag="KK030_26135"
     CDS             1887..2075
                     /locus_tag="KK030_RS26190"
                     /old_locus_tag="KK030_26135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_214575104.1"
                     /translation="MTKQESATLNMAKFIRAQSLLLLEKLDVLDLDDEAADCEQMHEQA
                     EALYQKLSARFGIQDGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2123..2350)
                     /locus_tag="KK030_RS26195"
                     /old_locus_tag="KK030_26140"
     CDS             complement(2123..2350)
                     /locus_tag="KK030_RS26195"
                     /old_locus_tag="KK030_26140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_206282062.1"
                     /translation="MFGFSPGELMNLCPDERLLFVRMISAMLRRSGGDAGAFMFEAYRH
                     IVSDTNQARRSYMLDLLESVRHDYVHGGYT"
                     /name="hypothetical protein"
     Typing          complement(2529..2702)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.429"
                     /coverage="100.0"
                     /evalue="5.6200000000000006e-73"
     Typing          2..162
                     /name="rep_cluster_2405"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.195"
                     /coverage="95.0"
                     /evalue="4.41e-44"
     Typing          2626..3062
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.091"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 atgcacgaac cccccgttca gtccgactgc tgcgcctttt ccggtaacta tcaacttgag
       61 tccaacccgg aaagacacga caaagcgcca ctggcagcag ccattggtaa ctggattagc
      121 agagcgggat aatgtaggca gtgctacaga gttcttgaag tggtggcctg agtgcggcta
      181 cactggaagg acagtttagg tatctgcgct ctgctgaagc cagttaccac ggttagaaaa
      241 tctgccagga tttactaacc ttcgaaaaac cacctgccag ggtggttttt tcgtttccaa
      301 ggcaagagat tacccgcaga gaaagacgat ctcaagaaga tcctttatca gcaacataca
      361 tcctcccgtc agtagtgagc ggggctagtg tcgtaatact gtcgttttat ttccatcaaa
      421 ttcaacacat acttcatgta cactctaatc acatgagaca ttaattatga acaaatcaaa
      481 tttccgtgcg ctgactgatg aggaagttgc taattgcgtc aagttaattc gacatgaccg
      541 aaaatggtct caggaacaat tggccgcaat cagtggatta aatttaagaa ctattcaaag
      601 agtagaaaaa ggagagcctt catctattga tacgcgaaga aacctagcaa attcttttgg
      661 tgcaaacgac attgatgctt tcaataaaga aacctatata ccatcgatgg atgaattaaa
      721 aaaataccaa gattatattg aggattcttg tgaacaaatc aaggcaacca aacttaacac
      781 aagcattggt cttctagacg caactgaagg atgtcacgca tcattcttcg attcatcctt
      841 cgatatgagt catgatgcag aggaagcaaa ccatctaatc aaatgctatt taaaagaata
      901 catggattgc catgatcttt ataacaaaga acagaaaaat gatgccgcaa aagaaatcca
      961 ggtacatata gacaaactga acaaactggg ggtggcactt gtttacgcaa agaaaaaaac
     1021 gaacataaca aacattggta ataacatatc aatggaaacc atttatataa tagcattccc
     1081 tattggtaag gaacctgaca cattaataat accgaatgaa gctgatgtga gtgtttagac
     1141 aagcacaatg actatatagt taatagattt accataacat ccgttgttcg aaccataatg
     1201 gcaggctata gcctgccgta attcaccgga aaaaatcatc caccgagtcg ctccactcat
     1261 gcccggaaag agaccacacc ttgtttatag ggggaggata accactcctg cagtacccgt
     1321 tttttcccac gaaatcgaat gccccgtgcg ggtatccatt gatcaggagt acgcacttct
     1381 gactgtcttc tgaccagcca attttaacat cagaaggaat gtgaccgtct gaaacgtctt
     1441 cggcattgta gatgtgtact gcgtccataa tcgggtttcc ctcgacggac tcatcgagcg
     1501 catagaaata cccggtccgg ccatcgtctt cgaacacggc ggccagcatg ccctcaggag
     1561 cgaggctctt tataacctgt tcctccccga caacgagctg ggcctgtatc gtcactaaaa
     1621 tggtcatcag gtctgttctc ctttccttgg ttgcttcggc agccagcttt cagacaagcg
     1681 gataatttac cataacatct gttgcgtgca ccgcctcaga gcctctgagg gcgtttctgc
     1741 ggcctcctgc ctgaaacata cacctttctg tgcccatatc gagaaaaaac gcccctgatg
     1801 atttctgcgt acctgcatgg ctatgcactc aagtgaatga agtaagattt cagtctgcga
     1861 tcaccataaa accgggaaaa agccgtatga caaaacagga atcagccaca ctgaacatgg
     1921 caaaatttat ccgggcgcag tcgctgctgc tgctcgaaaa actcgacgtg ctcgatcttg
     1981 atgacgaggc agccgactgt gagcaaatgc acgaacaggc agaagcgctt tatcagaagc
     2041 tgagcgcacg cttcggtata caggacggcg aataaagaga cgaaaagaac gctcacttag
     2101 acgaaaagcg tatccagggc aactatgtat atccaccgtg tacatagtca tgcctgacgc
     2161 tttcgagcag gtcgagcatg taggaacgac gagcttgatt ggtatcggac acaatatggc
     2221 gatacgcctc aaacataaaa gcacccgcat caccgcccga acggcgaagc atagcggaaa
     2281 tcatccgcac aaagagcaga cgttcatcgg ggcaaaggtt cataagttct cctggtgaaa
     2341 aaccaaacat gaagcaccca tctattaacg tcagctgtga acgcttcatt agaagaaaat
     2401 aggtaaaccc gggaaaaaga tccaaacgtt aacatcacag gtaaacagaa acgtcgggtc
     2461 gatcgggaaa ttctttcccg gacggcgcgg ggttgggcaa gccgcaggcg cgtcagtgct
     2521 tttagcaggt gtcggggcgc agccctgaac cagtcacgta gcgctagcgg agtgtatact
     2581 ggcttactat gttggcacgg atgcgaaaat cagtgaagtg cttcatgtgg aaggagaaaa
     2641 aaggcggctc cggcgcgtca gcagaatatg cgatacagga tatattccgc ttcctcgctc
     2701 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tgcgggctta ctggcggggc
     2761 ggaaatttcc tggaagatgc caggaagaaa cttaacaggg aagcgagaag gccacggcga
     2821 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
     2881 agtggtggcg aaacccgaca ggacttaaag ataccaggcg tttccccctg gtggctccct
     2941 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
     3001 tttatctcat tccacgcctg acactcggtt ccgggaaggc agttcgctcc aagctggact
     3061 gt
//
