LOCUS       NZ_CP116252             8252 bp    DNA     circular CON 27-FEB-2024
DEFINITION  Enterobacter roggenkampii strain 120063 plasmid p2_120063, complete
            sequence.
ACCESSION   NZ_CP116252
VERSION     NZ_CP116252.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN19316169
            Assembly: GCF_018596855.2
KEYWORDS    RefSeq.
SOURCE      Enterobacter roggenkampii
  ORGANISM  Enterobacter roggenkampii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Wei,L., Wang,C., Liu,L. and Zong,Z.
  TITLE     The complete genome of Enterobacter roggenkampii strain 120063
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8252)
  AUTHORS   Wei,L., Wang,C., Liu,L. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-2023) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 8252)
  AUTHORS   Wei,L., Wang,C., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2021) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Trycycler v. v0.5.3
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 150.0x
            Sequencing Technology :: Illumina Hiseq-X10; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/27/2024 02:58:09
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,285
            CDSs (total)                      :: 5,165
            Genes (coding)                    :: 4,946
            CDSs (with protein)               :: 4,946
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 219
            CDSs (without protein)            :: 219
            Pseudo Genes (ambiguous residues) :: 0 of 219
            Pseudo Genes (frameshifted)       :: 38 of 219
            Pseudo Genes (incomplete)         :: 148 of 219
            Pseudo Genes (internal stop)      :: 57 of 219
            Pseudo Genes (multiple problems)  :: 23 of 219
            Pseudo Genes (short protein)      :: 1 of 219
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116252.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8252
                     /organism="Enterobacter roggenkampii"
                     /mol_type="genomic DNA"
                     /strain="120063"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1812935"
                     /plasmid="p2_120063"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2019-05"
     gene            complement(join(7927..8252,1..822))
                     /locus_tag="KK030_RS26120"
                     /old_locus_tag="KK030_26065"
     CDS             complement(join(7927..8252,1..585,585..822))
                     /locus_tag="KK030_RS26120"
                     /old_locus_tag="KK030_26065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_115168574.1"
                     /translation="MSGKRYPEEFKIEAVKQVVDRGYSVASVATRLDITTHSLYSWIKK
                     YGPDSSTNKEQSDAQAEIRRLQKELKRVTDERDIFKKSRGVLRKAVRLRYAFIRDNTCC
                     WPVRLLCRVLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIHLDL
                     RDSGQQCGVNRVWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPDERWV
                     TDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPQKQVLV
                     HSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKKIYGTRE
                     EARSDIFDYIEMFYNSKRRHGSSDQMSPTEYENQYYQRLGSV"
                     /name="IS3 family transposase"
     gene            2179..4023
                     /locus_tag="KK030_RS26125"
     CDS             2179..4023
                     /locus_tag="KK030_RS26125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /GO_component="GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /GO_function="GO:0015474 - autotransporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0046819 - protein secretion by the type V
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="WP_271800764.1"
                     /translation="MTAPLLLVDSGQQDAVLLFDRNSNLTAARDGYLVDIIKYRDSIAA
                     SSLQMTLDNGSTMTGLTNKAYTNSTLNIDLNNNSTWNLAEKTTGAVSTSTFDALTMNGG
                     SKLNATTPRTAPANFILKGDVTSTLSTLNLADGKAGDVLTIQGNYKGNNGNLVLDTVLG
                     DDSSLTDKLVITGNASGTTTVTVNNANGSGASTLEGIEVIDTGSSTSNAFVQSGRIVAG
                     AHDYSLVRGAGSNINNWYLSSMVTPTPEPTPEPTPEPTPEPTPEPTPEPTPEPTPEPTP
                     EPKPVQTWRPELSSYTANLAAANTLFSTRLHDRLGETQYTDVLTGEKKVTSMWMRNVGG
                     HSQFTMNDGQNKTTANRYVLQIGGDVAQWSSDGLNRLHLGVMGGYANQRSSTKNNLTGY
                     RSKGSIDGYSAGLYGTWYQNDADKTGLYVDTWALYNWFDNDVTGDGLSAESYRSKGMTA
                     SVESGYTFHVGSQTTDKGMTNDFYLQPKMQLTWSGVKADDHTEVNGTRVQGSGNDNLQA
                     RIGTRFYLKGKSALDRDTQREFEPFVEANWIYNSRQYGTQMDGAGAAIQGSRNTGEVKA
                     GVEGRLTNNLNIWTSVGHQMGGDSYRDTQGQLGLKYLF"
                     /name="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(4209..4463)
                     /locus_tag="KK030_RS26130"
                     /old_locus_tag="KK030_26075"
     CDS             complement(4209..4463)
                     /locus_tag="KK030_RS26130"
                     /old_locus_tag="KK030_26075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /GO_process="GO:0008219 - cell death [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="WP_023157375.1"
                     /translation="MKLTWSAEAWEDYLYWQETDKKTVRKINELIKDASRTPFEGKGKP
                     EPLKHNLAGFWSRRITAEHRIVYAVSDDALLIASCRYHY"
                     /name="Txe/YoeB family addiction module toxin"
     gene            complement(4460..4711)
                     /gene="yefM"
                     /locus_tag="KK030_RS26135"
                     /old_locus_tag="KK030_26080"
     CDS             complement(4460..4711)
                     /gene="yefM"
                     /locus_tag="KK030_RS26135"
                     /old_locus_tag="KK030_26080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="WP_024176566.1"
                     /translation="MRAVSYSEARQNLSTTMLQTVEDRAPVLITRQNGESCVLMSLEEY
                     NSLEETAYLLRSPKNARRLMDSIESLKSGNGVEREIIE"
                     /name="yefM"
     gene            4961..5170
                     /locus_tag="KK030_RS26140"
                     /old_locus_tag="KK030_26085"
     CDS             4961..5170
                     /locus_tag="KK030_RS26140"
                     /old_locus_tag="KK030_26085"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_214575080.1"
                     /translation="MAMQPGEWYRLSARIPAESTVLSGLLQRRTIIRPLALRPFQLQRF
                     LVLRRALLLQVRLDTVQPCDNRRS"
                     /name="hypothetical protein"
     gene            complement(5143..5358)
                     /locus_tag="KK030_RS26145"
                     /old_locus_tag="KK030_26090"
     CDS             complement(5143..5358)
                     /locus_tag="KK030_RS26145"
                     /old_locus_tag="KK030_26090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_072048928.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5365..5862)
                     /locus_tag="KK030_RS26150"
                     /old_locus_tag="KK030_26095"
     CDS             complement(5365..5862)
                     /locus_tag="KK030_RS26150"
                     /old_locus_tag="KK030_26095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_024176569.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSTLLTASGASILWWQGQQIL
                     DNYTIIREQKDALEKLNARTWGVRYQEDNQGRFLVLPEGVKADTNWTFDNGRKNGIRLV
                     QE"
                     /name="MbeB family mobilization protein"
     gene            complement(<5897..6601)
                     /locus_tag="KK030_RS26470"
                     /pseudo=""
     CDS             complement(<5897..6601)
                     /locus_tag="KK030_RS26470"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(6540..6863)
                     /locus_tag="KK030_RS26160"
                     /old_locus_tag="KK030_26105"
     CDS             complement(6540..6863)
                     /locus_tag="KK030_RS26160"
                     /old_locus_tag="KK030_26105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_214575081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSL
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRNAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(7072..7275)
                     /locus_tag="KK030_RS26165"
                     /old_locus_tag="KK030_26110"
     CDS             complement(7072..7275)
                     /locus_tag="KK030_RS26165"
                     /old_locus_tag="KK030_26110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_077695987.1"
                     /translation="MEKRQRRILFQPFLISAKSLYPAIRELATPAAVSRQERSDRVSEE
                     ASYHLNLHFLLYALQLHFSPAT"
                     /name="hypothetical protein"
     gene            7505..7651
                     /locus_tag="KK030_RS26170"
                     /old_locus_tag="KK030_26115"
     CDS             7505..7651
                     /locus_tag="KK030_RS26170"
                     /old_locus_tag="KK030_26115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050863271.1"
                     /translation="MHEPPVQSDCCALSGNYHLESNPERHAKAPLAAANGKRMYKRLLT
                     QSS"
                     /name="hypothetical protein"
     Typing          complement(6965..7152)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.458"
                     /coverage="87.0"
                     /evalue="1.25e-50"
     Typing          7098..7614
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.595"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(5040..6550)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.27"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggttacgccg ccatacagcc atcagcagtg cgttcaggac aatgtctttt gtcatccggg
       61 attgcatgga ccagccgata attttgcgtg agaacagatc aacaaccacg gcaagataca
      121 gccagccttc gtgggtcctg atgtaggtta tgtccgttac ccaacgttca tccggagcat
      181 ccggattgaa ctgtcgctga agcctgttgg gcgacacgat actggcctcg cctttacgag
      241 cccgcgggct ccggtatccg acctgagcct ttatgccgac acgtttcatc agtcgccaga
      301 ctctgttcac tccgcactgt tgcccgctgt cccgcagatc cagatggatt ttgcgataac
      361 catagacgca tcccgattcc agccagaact gtttaatctg tcctgtcagt ctcaggtctg
      421 cctgatggcg ttgtgaatgc ggctgctgaa gccaggcgta aaaaccactg ggatgaacat
      481 ccagcacccg acagagcagg cgaacaggcc agcaacaggt gttgtcacgg ataaaggcgt
      541 acctcagtcg gacagctttg cgaagtacgc cgcggctttt tttaatatgt cccgttcgtc
      601 ggtaacccgc ttcagctctt tctggagacg gcggatctcg gcctgagcat ctgactgttc
      661 tttattagtg gaagaatccg gaccgtactt ctttatccag gagtaaaggc tgtgtgtggt
      721 gatatcgaga cgtgttgcaa cgctggcaac agaataaccg cgatcaacaa cctgtttgac
      781 tgcttcaatt ttaaactctt cgggataacg cttaccgctc atgggcacct ctctttaagt
      841 catcttaaat gactctgagg tgtctgttaa acctgtggcg attcaattat tctgtaacaa
      901 agaaaccaag agtaagggaa ctttcaacaa ccttcgtctg gaaatgaaaa aatgcctatt
      961 gttactgtct tagaaaaaat atacatcttg gaaagataca gtgcatgttt gctccgcgaa
     1021 aagtcgggaa gcacacagaa attagtccgt atctcagcga cagtcatcgc gctggtgacg
     1081 acaagcactc atggcgcttg ggcggtcacc tgcacagcag acgctaatgg gtcgtacatt
     1141 gctggatgga ccagtgctcc atatggcaat ggatgtaacg aagtgccagc aacaacagaa
     1201 tcaccgacag cattgggaaa aaacgcaagc tggtatgtat tagcacgtag caatctcgcc
     1261 gttggtgctg attccattac gatcgggaca acgaatatat catactcagg taccaacggt
     1321 ttcagcttaa tcggcaacca gtacctgacg aacggcagta tcaacttcac ggacgtgact
     1381 gcaaatctga ccaacaacgg taacggcggc ggtatcaacg gtctgagcac gcacagcgct
     1441 gtggcgatca acggaaataa tttcacactt acgacgaaca gcaattacac tggcttaaat
     1501 ttaagtaatg gcattgcctc ttacgccatc ctcgcaggat cttccgtgaa ctcaggtgaa
     1561 agcgcagtag ccaataatgg caagttttcg accatcacac tcaacaacgc aaccatacga
     1621 caaagcacta gcggtggatt ggtgcccgta ttgaacagcg gtctacgtgc catccaaggc
     1681 gcaaaccaaa atggcaacgg tagttcgggt aagattgaaa tcaaggacac gctggacatg
     1741 atactcaccg gtgctcgcat cgaaggaatc tatgtctcag gagccgcgtc gaacagtgcg
     1801 ggtaccgaag cggtatcgca ggtagttctg aacgacacca ctattaaaat ggtgaaaatc
     1861 ggtgcggcgt cttacgatag cagtgcaatc aagataggaa aggcccgagc tgtcggaagc
     1921 ggaaaaggac tcgttgtttc caacggtgca ttgaagattg acatggaccc aaattttgga
     1981 ggtagctcgg attacatgag ctccgccatc aagatatctc tcagcgggtc ggaactgcgg
     2041 gccaacggac ctaacagttc tgccgatatc aacgccaccc gatcagcttt ggccatcggc
     2101 atcgacgatt gggggacgag ttccgattcg agtggcatca aggctagctt tggtaaagcc
     2161 actataaaaa cacagtcgat gaccgcaccg ctcctgctgg tcgacagtgg tcaacaggac
     2221 gcggtgcttc tgtttgatcg aaactcaaac ctcaccgctg ctcgtgatgg ctacctagtc
     2281 gacataatca agtaccggga ctcgatagcg gccagttcgt tgcagatgac gctcgacaat
     2341 ggcagcacca tgacggggct gacgaacaag gcgtatacca actcgaccct gaacatagac
     2401 ctgaacaaca actcgacctg gaacctcgca gagaagacga cgggcgcagt ttcaacctcg
     2461 acattcgatg cactgacaat gaacggtggc tctaagctta acgccaccac gccgcgtact
     2521 gcgcctgcaa acttcatcct caagggcgac gtgacttcaa ccctctcaac tctgaacctc
     2581 gcggatggaa aagcaggaga cgttctcacc atccagggca actacaaggg caataacggt
     2641 aatctggttc tcgatacagt attaggtgat gactcttcat tgacggataa actagttatt
     2701 acaggtaatg catcaggcac aaccactgtc actgtgaata acgctaatgg ctcaggtgca
     2761 tcaaccttag aagggataga agttatcgat actggtagct caaccagtaa tgcgtttgtt
     2821 cagtctggcc gtattgttgc aggggcccac gattattctc ttgtgcgcgg agctggcagc
     2881 aatatcaaca actggtatct gagtagtatg gttacaccaa cgccagaacc aacgccagaa
     2941 ccaacgccag aaccaacgcc agaaccaacg ccagaaccaa cgccagaacc aacgccagaa
     3001 ccaacgccag aaccaacgcc agaaccaaaa ccggtgcaga cctggcgtcc tgagctgtcc
     3061 agttataccg ccaaccttgc ggccgcgaac acgctgttca gcacccgcct gcacgaccgc
     3121 ctgggtgaaa cccagtacac ggacgtgctg accggcgaga agaaagtgac gagcatgtgg
     3181 atgcgcaacg tgggcggtca cagccagttt acgatgaacg acggtcagaa taagacgacg
     3241 gctaaccgct acgttctgca gattggcggg gatgttgcgc agtggtcatc tgacggactg
     3301 aaccggcttc accttggcgt gatgggcggc tacgcaaacc agcgcagcag cacgaagaac
     3361 aacctgacag gctaccgctc gaagggaagc attgacggtt acagtgcggg tctttacggc
     3421 acctggtacc agaacgatgc agacaagacg gggctgtatg ttgacacctg ggcgctgtac
     3481 aactggtttg ataatgacgt gacgggtgat ggcctgagcg cggagtccta ccggagtaaa
     3541 gggatgacgg cctcggtgga gtcggggtac accttccacg tgggctcgca aaccacggac
     3601 aaggggatga ccaacgattt ctatctccag ccgaagatgc agctgacgtg gtccggcgtg
     3661 aaggcggatg accatacgga agtgaacggc acccgcgttc agggtagcgg gaatgacaat
     3721 cttcaggccc gcatcggcac ccgcttctac ctgaaaggga aaagcgccct ggacagggac
     3781 acccagcgtg agtttgagcc gttcgttgag gccaactgga tttataacag caggcagtac
     3841 ggcacgcaga tggacggagc cggcgcagcc attcagggca gccgcaacac gggagaggtt
     3901 aaggcgggcg tggaaggcag gctgacgaat aacctgaaca tctggacgtc ggtgggccat
     3961 cagatgggcg gagacagcta ccgggatacg caggggcagc tgggcctgaa atacctgttc
     4021 tgaccggact gacgtgaccg gaaaccccgg tcacgcacgc tgcgccacgc tcctgagtga
     4081 gcgtggcgtt ttactttcag ggggggggct gcgccataag cggcagcggc gaacgccatg
     4141 catggcgtta tccaggatga ttaaccggaa gtgattaaaa agtctccttc cggcggcttt
     4201 aggcatgatc agtagtgata acgacaggat gctatcagta aggcatcatc gcttacggca
     4261 taaacaatcc gatgctcagc agtgatcctc cgagaccaga atcccgcaag attatgcttc
     4321 agaggttccg gctttccctt tccctcaaaa ggcgtcctgc tggcatcttt gatgagctca
     4381 tttatttttc ttactgtttt tttgtccgtt tcctgccagt aaagataatc ttcccatgct
     4441 tcagcagacc acgtcagttt cactcaatga tttccctttc cacaccatta ccacttttca
     4501 ggctttctat tgagtccata agtctgcgcg cattcttcgg agaacgaagc agataagcgg
     4561 tctcttcgag agagttgtac tcctcaagtg acatcaacac gcatgattca ccgttctggc
     4621 gggtaataag tacgggagct ctgtcctcaa ccgtttgcag cattgttgta gacagattct
     4681 gccttgcttc actgtaactt actgctctca taatacctcc cttaatggat gtacaagtca
     4741 ttgtacatcc attaacccat ggaagaaagt aagcgcataa aacattcagc attgaagaat
     4801 taaaggcttc aggaaaacgt cttgaattaa acataacatc actttttggc cgcaccgccc
     4861 cggacgggtt cggcgcgaat gcggcatcag cccggcaaaa ccggcagtaa actccccgct
     4921 aaatcgcgca ttctgagcct gatagatttc acctggctgc atggctatgc agccaggtga
     4981 atggtaccgg ctttcggcgc ggatccccgc cgaaagcacc gttttatccg gtttgttaca
     5041 gcgacggacc atcatacgtc cgctggcgct gcgaccgttc cagctccagc gctttctggt
     5101 gctgcgccgc gcgctcctgc tccaggtgcg cctcgatacc gttcagcctt gcgacaaccg
     5161 gcgcagctga tcgctcaaac tctgcacctg cctgctcaag cccgtgacgc gctcgctcag
     5221 cgccgcgttc tcccgttgca taagaccaga catcgtccgc cattccgcga aggcgttctc
     5281 ccactcgtcc agccttttcg agtagtcctg ctgtagctgc tctaatgcgc tcagcaactg
     5341 tttttccagc tccgtcatgc tgctctactc ctgcaccagt ctgatgccgt tcttcctgcc
     5401 gttgtcgaac gtccagttcg tgtccgcttt cacgccttcc ggcagcacca gaaaacgccc
     5461 ctggttgtct tcctgatacc tcacgcccca cgtccgcgcg ttcagcttct ccagcgcgtc
     5521 cttctgctcc cggatgatcg tgtaattgtc gagtatctgc tgcccctgcc accatagaat
     5581 gcttgcgccc gatgctgtca gcaatgtaga caccaggacg atggtcagcc acgtctggct
     5641 gaccatacgc accatgcctt tcgtgcgctg gctcatggct gaggacagct tccggtcgtg
     5701 gtcgaggatg gcggcgctga ttctcttctc gctttcgctc agctccgctc tgacagactt
     5761 ctcgtgctcg ctgaatgcgg ctttcagcat ctcgccggta ctctgctgct gcgctttcga
     5821 tttctgctct aagtcctttg ccagcgttaa aagactgttc atagatggct cccttcagtc
     5881 ggatatttcg ccccccgtcc gggtcggcaa tgctgatgct gctttttgtg gtgcgtacca
     5941 cctcaaaacc tgcgctttcc agcgcctcag tgacgtcctg gcgggttttc agctccccgg
     6001 acgaggctaa ggccagtaaa ccccgtgtaa ttgcctgagc ggcttcctgc ttcgttttcg
     6061 gcagcgcgga cggcgtgacc agcgcccgcc ggttttcagg cgcgttcggg tcatgcagcc
     6121 ccagcctgcc gttcacgatg gtctgccagg catcaatgcg cggacggtcg gcgcggtcgt
     6181 aatacggctg gagacgcctg ccagtcagca gctccatgtt cgggatcagg aagttcagct
     6241 ccagccgccc cttatcctgg tgctcaaccc acagcacgct gtactggtct ttatcgagtc
     6301 cgggcatcag aacccgttca aagctcgcca tcagcttttc gcgctgcccg ggcggtaaat
     6361 tcttttcggc aaaggacagg acgccggatg tgtacttttt tgcgtagggc gaggcgtcga
     6421 taagctccct gacttcttcc ggcttcccct gcagaacggt ggcgccgtcg cgctgtcggt
     6481 ctttccccag cagataatcc accggaccgg cgccaccgcc gcgccctcgc gggtgaaact
     6541 taacgatcat cgtctgcacc cttttccagc acggcattcc gcagccgctc gagtccggca
     6601 tctatggcca tcagcgcggc gacgacctgc acgcggtcgt gcccgctgcc accaccggcg
     6661 ttaacctgcc gggcaatctg gttgaggttg ttccccatgc cggcaagctg acgaagcagc
     6721 gccggcgaga gcgagggaag cttgccggcg cgggcgggtt tctcgtccag gcacgtctgg
     6781 cgcatccagg ctgcaagctg tttaccgtcg cagcgttcca acagacggcg gtgctcatct
     6841 tcggtcaccc acatcgtgag cattttactg cgtttgtctg ccaatccagc ctcctgataa
     6901 cgccgggacg ggcgggaaat cttttcccgg tcggcgcggt gttggacaag ccgcaggcgc
     6961 gtcagtggtt ttagcaggtt tcggggcgca gccctgaacc agtcacgtag cgctagcgga
     7021 gtgtatactg gctcagtatg ttacggcgga gtaacactta atgaaagtgc ttcatgtggc
     7081 aggggaaaaa tgcagctgca gggcgtacag gagaaaatgg aggttaaggt gatatgacgc
     7141 ctcctcgctc actcggtcgc tgcgctcctg ccgtgagact gcggcgggcg ttgccagctc
     7201 acgaatggct ggataaagag acttagcgga aatcaggaag ggctgaaaaa ggatgcggcg
     7261 ttgccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat
     7321 cagaggcggc gaaacccgac aggactataa agatcccagg cgtttccccc tggtagctcc
     7381 ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct gttatggccg
     7441 cgtttatctc attccacgcc tgacactcag ttccgggtag gcagttcgct ccaagctgga
     7501 ctgtatgcac gaaccccccg ttcagtccga ctgctgcgcc ttatccggta actatcatct
     7561 tgagtccaac ccggaaagac atgcaaaagc gccactggca gcagccaacg gtaagaggat
     7621 gtacaagaga ttactaacgc agagttcttg aagtatcagc ccaactacgg ctaaactgga
     7681 aggacgaatt tggtggcagt ggtcttgtac accctgttac cacggttcat aaacttgccg
     7741 gtttaatgaa ccttcgaaaa accaccttgc cgggtggttt tttcgttttc aaagaagata
     7801 cgcgtttact cttcgcctcg tcaatgaagg gtcgcattaa ttctgcgact tgcgcatctc
     7861 ttctacaata cagtacagtt actatgaagt tgacgtatcc gtcacctctg aatcgccacg
     7921 gataatttag acacttccga gccgttgata atactggttt tcatattctg tcggtgacat
     7981 ctgatcgcta gaaccatgcc gacgtttact gttataaaac atttcgatgt aatcaaaaat
     8041 atcgctgcgg gcttcttccc gcgttccgta gatctttttc tttatccgtt cgcgtttcaa
     8101 caactggaaa aagctttctg caaccgcatt atcatggcag ttaccgcgac ggctcatgct
     8161 gccctccagg ccgtgtgatt tcaggaacga ctgccattca tggcttgtgt actgactgcc
     8221 ctgatccgaa tgaaccagca cctgtttttg gg
//
