LOCUS       NZ_CP116244            11610 bp    DNA     circular CON 25-FEB-2024
DEFINITION  Escherichia coli strain 7.1994/NIST0056 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP116244
VERSION     NZ_CP116244.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32743768
            Assembly: GCF_027947615.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11610)
  AUTHORS   Stabryla,L., Servetas,S. and Kralj,J.
  TITLE     NIST0056
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11610)
  AUTHORS   Stabryla,L., Servetas,S. and Kralj,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JAN-2023) Biosystems and Biomaterials, NIST, 100
            Bureau Drive, Gaithersburg, MD 20899, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Flye v. v2.5; Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 26.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/25/2024 02:20:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,055
            CDSs (total)                      :: 4,935
            Genes (coding)                    :: 4,734
            CDSs (with protein)               :: 4,734
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 201
            CDSs (without protein)            :: 201
            Pseudo Genes (ambiguous residues) :: 0 of 201
            Pseudo Genes (frameshifted)       :: 103 of 201
            Pseudo Genes (incomplete)         :: 99 of 201
            Pseudo Genes (internal stop)      :: 33 of 201
            Pseudo Genes (multiple problems)  :: 33 of 201
            Pseudo Genes (short protein)      :: 4 of 201
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116244.1.
            Bacteria and source DNA available from Penn State E. coli Reference
            Center (ECRC); NIST.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="7.1994/NIST0056"
                     /host="avian"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: Missouri"
                     /lat_lon="37.96 N 91.83 W"
                     /collection_date="2007"
                     /collected_by="Penn State E. coli Reference Center (ECRC)"
     gene            374..835
                     /locus_tag="PH192_RS24650"
                     /old_locus_tag="PH192_24645"
     CDS             374..835
                     /locus_tag="PH192_RS24650"
                     /old_locus_tag="PH192_24645"
                     /inference="COORDINATES: protein motif:HMM:NF013235.4"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_271356499.1"
                     /translation="MSKGNLVVKTNQLNSALQNLSLPEIRIVQLAIVDARETNTGLSTD
                     KPLRIDALRYAEMFETTRQNGYKRMKEAEETLFNRRFSYIDDKGKVIKSRWIQQVRYLD
                     DEGAIELVFTLAVVNGISRIDGAEDFLPHTCLSKPPVWIVFTPLGFMNC"
                     /name="RepB family plasmid replication initiator protein"
     gene            775..939
                     /locus_tag="PH192_RS24655"
                     /old_locus_tag="PH192_24650"
     CDS             775..939
                     /locus_tag="PH192_RS24655"
                     /old_locus_tag="PH192_24650"
                     /inference="COORDINATES: protein motif:HMM:NF013235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_333782063.1"
                     /translation="MLEQTASMDSIYSVRLYELLVQWKAAKQTPMFALEKFRDQLGVGI
                     EEYKAMNDF"
                     /name="hypothetical protein"
     gene            932..994
                     /locus_tag="PH192_RS25320"
     CDS             932..994
                     /locus_tag="PH192_RS25320"
                     /inference="COORDINATES: protein motif:HMM:NF044726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_333782062.1"
                     /translation="MFKKRVLLVAIDEINEKIRY"
                     /name="hypothetical protein"
     gene            966..1322
                     /locus_tag="PH192_RS24660"
                     /old_locus_tag="PH192_24655"
     CDS             966..1322
                     /locus_tag="PH192_RS24660"
                     /old_locus_tag="PH192_24655"
                     /inference="COORDINATES: protein motif:HMM:NF044726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_271356496.1"
                     /translation="MKSTKKSDIKISYEQVKKGRKIIGFTFTVHEKPKAKADVVSKERD
                     ASTADMFTVDGLNDKQLGRIARNPSFIADYNHLVSSTSPAGQDPNAWEFEMINRLKKDP
                     EQFKKRPIREYLEY"
                     /name="hypothetical protein"
     gene            complement(1513..2210)
                     /locus_tag="PH192_RS24665"
                     /old_locus_tag="PH192_24660"
                     /pseudo=""
     CDS             complement(1513..2210)
                     /locus_tag="PH192_RS24665"
                     /old_locus_tag="PH192_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959554.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            complement(3284..3550)
                     /locus_tag="PH192_RS24670"
                     /old_locus_tag="PH192_24665"
                     /pseudo=""
     CDS             complement(3284..3550)
                     /locus_tag="PH192_RS24670"
                     /old_locus_tag="PH192_24665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917961.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family toxin"
                     /name="type II toxin-antitoxin system YafQ family toxin"
     gene            complement(3537..3821)
                     /locus_tag="PH192_RS24675"
                     /old_locus_tag="PH192_24670"
     CDS             complement(3537..3821)
                     /locus_tag="PH192_RS24675"
                     /old_locus_tag="PH192_24670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02384.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="WP_191692553.1"
                     /translation="MTTTNYNIRLDQDLKDRSFSVLESYGLTPSQAIRLFLNQVADTNA
                     VPLSFDYQQPKLNAQTIAAIAEVASGKGTKYDSFGNLMDELEGEADEIH"
                     /name="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     gene            complement(3936..4187)
                     /locus_tag="PH192_RS24680"
                     /old_locus_tag="PH192_24675"
     CDS             complement(3936..4187)
                     /locus_tag="PH192_RS24680"
                     /old_locus_tag="PH192_24675"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_271356501.1"
                     /translation="MSMFGVSVASSKSLQLEATQEAYNRAVVKLNLLLIDDKTHEEVVR
                     SKLFEVMDERNQLGKYSTSDLYVMQKALRKRLTSFFLD"
                     /name="hypothetical protein"
     gene            4891..5070
                     /locus_tag="PH192_RS24685"
                     /old_locus_tag="PH192_24680"
     CDS             4891..5070
                     /locus_tag="PH192_RS24685"
                     /old_locus_tag="PH192_24680"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_271356502.1"
                     /translation="MGRLGEKSQEVEFFKRSAQWLIDQHGEENIAGMSIHRDISTPHLV
                     AYVVPIDQKGEAQL"
                     /name="plasmid recombination protein"
     gene            5106..5426
                     /locus_tag="PH192_RS24690"
                     /old_locus_tag="PH192_24685"
     CDS             5106..5426
                     /locus_tag="PH192_RS24690"
                     /old_locus_tag="PH192_24685"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_271356503.1"
                     /translation="MQTDFANKVADLGLMRGKEGSKAKHTSIKEYYHDINHARDFSITT
                     VSLKPEMFESKARYGEKVTAAVIEQIEPTVKAANSILMDYEKARLDKSVAEASYNSLKK
                     TC"
                     /name="hypothetical protein"
     gene            6175..6741
                     /locus_tag="PH192_RS24695"
                     /old_locus_tag="PH192_24690"
     CDS             6175..6741
                     /locus_tag="PH192_RS24695"
                     /old_locus_tag="PH192_24690"
                     /inference="COORDINATES: protein motif:HMM:NF013235.4"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_271356495.1"
                     /translation="MSKGNLVVKTNQLNSALQNLSLPEIRIVQLAIVDARETNTGLSTD
                     KPLRIDALRYAEMFETTRQNGYKRMKEAEETLFNRRFSYIDDKGKVIKSRWIQQVRYLD
                     DEGAIELVFTLAVVNGISRIDGAEDFFTSYLLEQTASMDSIYSVRLYELLVQWKAAKQT
                     PMFALEKFRDQLGVGIEEYKAMNDF"
                     /name="RepB family plasmid replication initiator protein"
     gene            6734..6796
                     /locus_tag="PH192_RS25325"
     CDS             6734..6796
                     /locus_tag="PH192_RS25325"
                     /inference="COORDINATES: protein motif:HMM:NF044726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_333782062.1"
                     /translation="MFKKRVLLVAIDEINEKIRY"
                     /name="hypothetical protein"
     gene            6768..7124
                     /locus_tag="PH192_RS24700"
                     /old_locus_tag="PH192_24695"
     CDS             6768..7124
                     /locus_tag="PH192_RS24700"
                     /old_locus_tag="PH192_24695"
                     /inference="COORDINATES: protein motif:HMM:NF044726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_271356496.1"
                     /translation="MKSTKKSDIKISYEQVKKGRKIIGFTFTVHEKPKAKADVVSKERD
                     ASTADMFTVDGLNDKQLGRIARNPSFIADYNHLVSSTSPAGQDPNAWEFEMINRLKKDP
                     EQFKKRPIREYLEY"
                     /name="hypothetical protein"
     gene            7198..7347
                     /locus_tag="PH192_RS24705"
                     /old_locus_tag="PH192_24700"
     CDS             7198..7347
                     /locus_tag="PH192_RS24705"
                     /old_locus_tag="PH192_24700"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_271356505.1"
                     /translation="MSNKKFFMRAIGVCGLIFILSTMVAARFFGGGSKSRLTLDIPMID
                     VKES"
                     /name="hypothetical protein"
     gene            complement(7312..8018)
                     /locus_tag="PH192_RS24710"
                     /old_locus_tag="PH192_24705"
                     /pseudo=""
     CDS             complement(7312..8018)
                     /locus_tag="PH192_RS24710"
                     /old_locus_tag="PH192_24705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011959554.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element ISPssp2 family transposase"
                     /name="IS1-like element ISPssp2 family transposase"
     gene            complement(9093..9359)
                     /locus_tag="PH192_RS24715"
                     /old_locus_tag="PH192_24710"
                     /pseudo=""
     CDS             complement(9093..9359)
                     /locus_tag="PH192_RS24715"
                     /old_locus_tag="PH192_24710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004917961.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family toxin"
                     /name="type II toxin-antitoxin system YafQ family toxin"
     gene            complement(9346..9630)
                     /locus_tag="PH192_RS24720"
                     /old_locus_tag="PH192_24715"
     CDS             complement(9346..9630)
                     /locus_tag="PH192_RS24720"
                     /old_locus_tag="PH192_24715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02384.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="WP_191692553.1"
                     /translation="MTTTNYNIRLDQDLKDRSFSVLESYGLTPSQAIRLFLNQVADTNA
                     VPLSFDYQQPKLNAQTIAAIAEVASGKGTKYDSFGNLMDELEGEADEIH"
                     /name="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     gene            complement(9721..9996)
                     /locus_tag="PH192_RS24725"
                     /old_locus_tag="PH192_24720"
     CDS             complement(9721..9996)
                     /locus_tag="PH192_RS24725"
                     /old_locus_tag="PH192_24720"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_191692554.1"
                     /translation="MSMFGVSVASSKSLQLEATQEAYNRAVVKLNLLLIDDKTHEEVVR
                     SKLFEVMDERNQLGKYSTSDLYVMQKSIEKTVDEFLSGLNEQTITA"
                     /name="hypothetical protein"
     gene            10555..10743
                     /locus_tag="PH192_RS24730"
                     /old_locus_tag="PH192_24725"
     CDS             10555..10743
                     /locus_tag="PH192_RS24730"
                     /old_locus_tag="PH192_24725"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_271356497.1"
                     /translation="MLTPTSPPKNHHSVATPEGVKQAIKDCLPEKRRSDAVLCIEYLIT
                     ASPEWEGWGKKPRSRIF"
                     /name="hypothetical protein"
     gene            10763..10879
                     /locus_tag="PH192_RS24735"
                     /old_locus_tag="PH192_24730"
     CDS             10763..10879
                     /locus_tag="PH192_RS24735"
                     /old_locus_tag="PH192_24730"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_271356504.1"
                     /translation="MDQHGEENIAGMSIHRDISTPHLVAYVVPIDQKGEAQL"
                     /name="hypothetical protein"
     gene            10915..11121
                     /locus_tag="PH192_RS24740"
                     /old_locus_tag="PH192_24735"
     CDS             10915..11121
                     /locus_tag="PH192_RS24740"
                     /old_locus_tag="PH192_24735"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_271356498.1"
                     /translation="MQTDFANKVADLGLMRGKEGSKAKHTSIKEYYHDINHARDFSITT
                     VSLKPEMFESKARYGGKGHSSRY"
                     /name="hypothetical protein"
     Typing          5050..5417
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="82.927"
                     /coverage="93.0"
                     /evalue="3.55e-64"
ORIGIN
        1 tcttctggct ttttaattaa taaaaaaaca aaaaaatgct tttaatgctt ttaaatgctt
       61 ttaaatgctt ttagaccctc tgtgaccctt actactatta gcttgtaagc tccattaagg
      121 gacgtttagt tacccattaa gggacgttta gttacccatt aagggacgtt tagttaccca
      181 ttaagggacg tttagttacc attattggac ttatatatat ttgtcccttt aatgacgttt
      241 agttaccatt attggactta tatatatttg tccctttaat gacgtttagt taccattgtt
      301 ggacttatat atatttgtcc cataagggac atagtgttat tatgtcacta aataatagta
      361 gagaagactt ctcgtgagta aaggtaattt agtcgttaaa acaaatcagc ttaattcagc
      421 attacagaat ttatctttgc ctgaaattcg tattgttcaa ctggcaatag tagatgctag
      481 ggaaaccaat acagggctaa gcactgataa accattacgc attgatgctt tacgctatgc
      541 tgagatgttt gaaactactc gacagaatgg ctataagcgt atgaaagaag ctgaggagac
      601 gttatttaat aggcgtttca gctatattga cgacaaaggg aaagttatta aatctcgttg
      661 gatacagcaa gtaagatatc ttgatgacga gggcgcaatc gaattagtct ttactcttgc
      721 cgtggtcaat ggcattagcc gtattgatgg tgcagaagat tttttacctc atacctgctt
      781 gagcaaaccg ccagtatgga tagtatttac tccgttaggc tttatgaatt gttagtacag
      841 tggaaagctg ccaagcagac tccgatgttt gcgcttgaga agtttagaga tcaattgggt
      901 gttggtatcg aagaatataa agctatgaac gatttttaaa aagcgtgtac tactagtagc
      961 tattgatgaa atcaacgaaa aaatcagata ttaaaatcag ctatgaacaa gttaaaaaag
     1021 gtcgcaagat tataggtttt actttcactg ttcatgaaaa gccaaaagct aaggctgatg
     1081 tagttagtaa ggagcgcgac gctagtacag cagatatgtt tacggttgac ggattgaatg
     1141 ataagcagct agggcgtatt gcccgtaatc ctagctttat tgctgattat aatcatttgg
     1201 ttagttctac cagtccagca ggtcaagatc cgaatgcttg ggagtttgaa atgattaacc
     1261 gccttaaaaa agaccctgag cagtttaaaa agcgccctat tagagaatat cttgaatact
     1321 agatcttatg agagcgttcg ctagatatga ttcccctact gagggcttaa ggtgagtatt
     1381 tcgaggaaat ttggcattga gcaataaaaa aattttttat gcgagcaatt ggggtttgtg
     1441 gtctaatttt ttattttgtc tacgatggtt gctgctagat tttttggtgg tggttcaaaa
     1501 agtaggctaa cattagacat acccatgatt gatgtaaaag aaagctaaat caaaggcttt
     1561 aaagtgattt tccagttttt ttagaaaagt tacaacttcg cctaaatgct cgccttactc
     1621 gatgtctgat agtgcaatta ttgccttcaa tgccgaccgt gaaaaacttg ccgatgactt
     1681 gggtaaagcc cttaaaacca gtaacaaagc tatcccaggt atcactagcg attctagcac
     1741 aactgacgcc tagggctttc agcttagctt taagcttttt gacagtgtta aggtctcgtt
     1801 ttccccacac ataggcaaca atttcacctg tgtcacggtg ataggcataa attagccatt
     1861 gtttgttggt ctttttacct acgaatgtcc aaagctcatc aatttcaaga cagtcgtatt
     1921 gcctttgctt gggggggtta tttggtagtg acactttttt agggtggcca gtactttgcc
     1981 tttgctgatt ctctagcgat gtcttttatg ccactgcctc gaaccataag atgtcgtatt
     2041 ttgctatctt tttgggagtg acagccttgg tacgttaagg catggtcgcc aataaactgc
     2101 cgtttgcagt ctttgcattt atagttttgt ttaccatagc ttttgaagcc atttttcttt
     2161 atattgtcac ttagacaggc tgggcatttg atgtatagtg tgatgtgcat ttcactattt
     2221 tatcaatttc tcagcctgtt ttttttccca gcctactttt tgaaccacca ccgatttttt
     2281 ttagtatgtt tcctacaata gaacataaac tgaaagctgt atacctagtg tttacggctt
     2341 ttctagagtt gctggacagg taaaagcggt catcgggagg gagtatcctt tgtagcttca
     2401 atccgcatgt gaacggcacg ctctccaatc tcaaaaagtt gagtctccaa ctatacccta
     2461 gcgtttaagc ttgaatatag ggctacctat agtttttttg tttcataata gatatagcgt
     2521 cttcgtagaa gtctatatga agtatctcgc tatctttttt aggcgctaga tttaactcat
     2581 tggtgagagt tctctgccca aaattatatt ttggcccttc agacggctgc ttgccacaac
     2641 gaatacattg ataagaaact cctctttcct cagtagggac cgctaatgaa ccacagcggc
     2701 accggataga atacgtttct aatatcctaa cattgataag gatcgggcta ctcagagtct
     2761 cagcaataga ttttttatgt ttacgcgcaa atactgattg gatcttttta atcattattc
     2821 tcaattccta gaggtagtta tctataaaac acttttagta ctttataatt ttttacaaaa
     2881 taattgaata ggaattctac tcagagaata acgaatataa gccctgaaaa tactgggctt
     2941 aacagacttt aattgtatcg gtaataatat aaagtattaa ttacattgta actcttttgt
     3001 actttttaag taaacaaaat aaacataaac tgctttgacc tatacggtca atcccgtagg
     3061 aattgatccc aagttaagtg agatatttag ataataaagg agtgctcgaa gtagtattta
     3121 ctccagcaat ggtaagagag attaatggcg ctaaagagtt cttactaaat atttattaga
     3181 actgccaagc taactacagt actttggaaa acctacgaga ttaggcattt tcccctgcct
     3241 tggctatctc ttaatccaac aaccaaaggg cgttagcttt ggactaatca aatatctctg
     3301 aatggctacc cagcctaaca aaatctaccg catcgctttg aatacggtag ataagcacca
     3361 aatcaggttt gatatggcaa tctctaaatc ccttatgatt gccggtcagc gcatgatctt
     3421 tgtacttagc tggtagcggc atatcgttag tcaaacaatt aagcacctca cccccatgca
     3481 ggagtgagca agcttagata attcttttgg catctctctt aaactgactt gatacttcaa
     3541 tggatctcat cagcctcgcc ttctagctca tccatcaaat taccaaagct atcgtactta
     3601 gtgcctttac cgctcgcaac ctcagcaata gccgcgatag tttgcgcgtt gagcttaggt
     3661 tgttgatagt caaaagacaa gggaactgca ttggtatcgg ctacttggtt caagaacagt
     3721 cttatggctt gtgaaggcgt tagtccatag ctttcaagaa cagaaaaaga cctgtccttc
     3781 agatcctggt caagccgtat attataatta gttgtagtca tagcaatcac ctttcgctta
     3841 tattcgtacc ccccaatata agctaaaggt gactaaacct caataacttt agcattctat
     3901 cttctcgcta atctaagcgg taatagtctg ctcattcaat ccagaaagaa actcgtcaac
     3961 cgttttctca atgctttttg catcacataa agatcagaag tggagtattt gccaagctga
     4021 tttctttcat ccatgacttc aaacagcttg cttctgacca cttcttcgtg cgttttatcg
     4081 tcaattaaca gtaaattaag cttcacgaca gctctattgt aggcttcttg agtggcttca
     4141 agttgaaggc ttttactgct agctactgat acaccgaaca tgctcataaa taactccgct
     4201 actagaatca tctgtagcta cagatgattt tttttaggtg aataaattat tgataccagc
     4261 ctaagattat gccagtgaat gactggaagt aacgctctta taagcccctt ttttgtagca
     4321 gattttacga cagataaaaa aatccgttat ttcccaccta atttaatgac tacaccttac
     4381 gcaccctaaa gggactgcca aatagctgat attgttaggt ttattaaaat ttgagtgttt
     4441 gcaaagtgca aacaaaatca ttataagtta ttgatttaaa aagggaaaaa ggtgtttaca
     4501 atctgtaaac acctaagttg aaaaacagtg aaaaacagcc ccctttttac cgccttttgc
     4561 agcaaaaatc tcgctttcct aacaggtgaa tttatggtgt agtaggtaca ctttagtaaa
     4621 ataacaccca attatcagga aaaagtagta atgaactatg cgattttgag gacgacaaaa
     4681 ttaaagacga tgggcaacat tggtggcagt cttgcacaca gctatcgaac gatagaaacg
     4741 ccaaatgctg accccaacct caccccctaa aaatcatcat agcgttgcta cccctgaagg
     4801 ggttaaacaa gcaataaaag attgtctccc tgaaaaaaga cgctctgatg cggtgctctg
     4861 tattgagtat ttaataaccg ccagtcccga atgggaaggc tgggggaaaa aagccaagaa
     4921 gtagaatttt ttaagcggtc tgctcagtgg ctcattgatc agcatgggga agaaaacatt
     4981 gcaggtatga gtattcatcg agacataagc acaccgcatc tagtcgctta tgtcgtacct
     5041 attgaccaaa aaggggaagc tcaactgtaa gaatttttct aggcggtagg gctaaattaa
     5101 acaagatgca aaccgatttt gctaataagg tcgctgatct agggctaatg aggggcaaag
     5161 aaggctctaa agccaagcat accagcatca aggaatatta ccacgacatc aaccatgcta
     5221 gagattttag tattacaacc gtatcactta agcctgaaat gtttgaaagc aaggctagat
     5281 atggggaaaa ggtcacagca gccgttattg agcaaataga accgacggta aaagcggcaa
     5341 acagcatcct aatggactat gagaaagcta gattggataa aagcgttgcg gaagcgagct
     5401 ataactctct caaaaaaacg tgttgagcct tacttactgg cgactgaagg gttaaatcga
     5461 gaggagatca ttagattgaa tgaggcaatg cagatagaaa gctgtagaat agctgtagag
     5521 agtgaaaaaa tttaaaatag ctatacatgt ataactatag agatagttag cctttaaatc
     5581 gaaatctgtg aacaaataga gtggttttta tatctgatca ctaaggttta agaaaaaata
     5641 cagtcattag aatactgaaa aaacttaaaa agttcaacaa acctgctgtt ttgcattaag
     5701 ttaaaacaaa ttgatggata agtttgaaag tattaatcga ttatctgatc caaataagca
     5761 taaaaaagtc cctgatattt tgaggtaact aaggcttttc tcttctggct ttttaattaa
     5821 taaaaaaaac aaaaaaatgc ttttaatgct tttaaatgct tttaaatgct tttagaccct
     5881 ctgtgaccct tactactatt agcttgtaag ctccattaag ggacgtttag ttacccatta
     5941 agggacgttt agttacccat taagggacgt ttagttaccc attaagggac gtttagttac
     6001 cattattgga cttatatata tttgtccctt taatgacgtt tagttaccat tattggactt
     6061 atatatattt gtccctttaa tgacgtttag ttaccattgt tggacttata tatatttgtc
     6121 ccataaggga catagtgtta ttatgtcact aaataatagt agagaagact tctcgtgagt
     6181 aaaggtaatt tagtcgttaa aacaaatcag cttaattcag cattacagaa tttatctttg
     6241 cctgaaattc gtattgttca actggcaata gtagatgcta gggaaaccaa tacagggcta
     6301 agcactgata aaccattacg cattgatgct ttacgctatg ctgagatgtt tgaaactact
     6361 cgacagaatg gctataagcg tatgaaagaa gctgaggaga cgttatttaa taggcgtttc
     6421 agctatattg acgacaaagg gaaagttatt aaatctcgtt ggatacagca agtaagatat
     6481 cttgatgacg agggcgcaat cgaattagtc tttactcttg ccgtggtcaa tggcattagc
     6541 cgtattgatg gtgcagaaga tttttttacc tcatacctgc ttgagcaaac cgccagtatg
     6601 gatagtattt actccgttag gctttatgaa ttgttagtac agtggaaagc tgccaagcag
     6661 actccgatgt ttgcgcttga gaagtttaga gatcaattgg gtgttggtat cgaagaatat
     6721 aaagctatga acgattttta aaaagcgtgt actactagta gctattgatg aaatcaacga
     6781 aaaaatcaga tattaaaatc agctatgaac aagttaaaaa aggtcgcaag attataggtt
     6841 ttactttcac tgttcatgaa aagccaaaag ctaaggctga tgtagttagt aaggagcgcg
     6901 acgctagtac agcagatatg tttacggttg acggattgaa tgataagcag ctagggcgta
     6961 ttgcccgtaa tcctagcttt attgctgatt ataatcattt ggttagttct accagtccag
     7021 caggtcaaga tccgaatgct tgggagtttg aaatgattaa ccgccttaaa aaagaccctg
     7081 agcagtttaa aaagcgccct attagagaat atcttgaata ctagatctta tgagagcgtt
     7141 cgctagatat gattccctac tgagggctta aggtgagtat ttcgaggaaa tttggcattg
     7201 agcaataaaa aattttttat gcgagcaatt ggggtttgtg gtctaatttt tattttgtct
     7261 acgatggttg ctgctagatt ttttggtggt ggttcaaaaa gtaggctaac attagacata
     7321 cccatgattg atgtaaaaga aagctaaatc aaaggcttta aagtgatttt ccagtttttt
     7381 tagaaaagtt acaacttcgc ctaaatgctc gccttactcg atgtctgata gtgcaattat
     7441 tgccttcaat gccgaccgtg aaaaaacttg ccgatgactt gggtaaagcc cttaaaacca
     7501 gtaacaaagc tatcccaggt atcactagcg attctagcac aactgacgcc tagggctttc
     7561 agcttagctt taagcttttt gacagtgtta aggtctcgtt ttccccacac ataggcaaca
     7621 atttcacctg tgtcacggtg ataggcataa attagccatt gtttgttggt ctttttacct
     7681 acgaatgtcc aaagctcatc aatttcaaga cagtcgtatt gcctttgctt gggggggtta
     7741 tttggtagtg acactttttt agggtggcca gtactttgcc tttgctgatt ctctcaacgc
     7801 aagcgatgtc ttttatgcca ctgcctcgaa ccataagatg tcgtattttg ctatcttttt
     7861 gggagtgaca gccttggtac gttaaggcat ggtcgccaat aaactgccgt ttgcagtctt
     7921 tgcatttata gttttgttta ccatagcttt tgaagccatt ttttctttat attgtcactt
     7981 agacaggctg ggcatttgat gtatagtgtg atgtgcattt cactatttta tcaatttctc
     8041 agcctgtttt ttttcccaac ctactttttg aaccaccacc gatttttttt agtatgtttc
     8101 ctacaataga acataaactg aaagctgtat acctagtgtt tacggctttt ctagagttgc
     8161 tggacaggta aaagcggtca tcgggaggga gtatcctttg tagcttcaat ccgcatgtga
     8221 acggcacgct ctccaatctc aaaaagttga gtctccaact ataccctagc gtttaagctt
     8281 gaatataggg ctacctatag ttttttttgt ttcataatag atatagcgtc ttcgtagaag
     8341 tctatatgaa gtatctcgct atctttttta ggcgctagat ttaactcatt ggtgagagtt
     8401 ctctgcccaa aattatattt tggcccttca gacggctgct tgccacaacg aatacattga
     8461 taagaaactc ctctttcctc agtagggacc gctaatgaac cacagcggca ccggatagaa
     8521 tacgtttcta atatccctaa cattgataag gatcgggcta ctcagagtct cagcaataga
     8581 ttttttatgt ttacgcgcaa atactgattg gatcttttta atcattattc tcaattccta
     8641 gaggtagtta tctataaaac acttttagta ctttataatt tttacaaaat aattgaatag
     8701 gaattctact cagagaataa cgaatataag ccctgaaaat actgggctta acagacttta
     8761 attgtatcgg taataatata aagtattaat tacattgtaa ctcttttgta ctttttaagt
     8821 aaacaaaata aacataaact gctttgacct atacggtcaa tcccgtagga attgatccca
     8881 agttaagtga gatatttaga taataaagga gtgctcgaag tagtatttac tccagcaatg
     8941 gtaagagaga ttaatggcgc taaagagttc ttactaaata tttattagaa ctgccaagct
     9001 aactacagta ctttggaaaa cctacgagat taggcatttt cccctgcctt ggctatctct
     9061 taatccaaca accaaagggc gttagctttg gactaatcaa atatctctga atggctaccc
     9121 agcctaacaa aatctaccgc atcgctttga atacggtaga taagcaccaa atcaggtttg
     9181 atatggcaat ctctaaatcc cttatgattg ccggtcagcg catgatcttt gtacttagct
     9241 ggtagcggca tatcgttagt caaacaatta agcacctcac ccccatgcag gagtgagcaa
     9301 gcttagataa ttcttttggc atctctctta aactgacttg atacttcaat ggatctcatc
     9361 agcctcgcct tctagctcat ccatcaaatt accaaagcta tcgtacttag tgcctttacc
     9421 gctcgcaacc tcagcaatag ccgcgatagt ttgcgcgttg agcttaggtt gttgatagtc
     9481 aaaagacaag ggaactgcat tggtatcggc tacttggttc aagaacagtc ttatggcttg
     9541 tgaaggcgtt agtccatagc tttcaagaac agaaaaagac ctgtccttca gatcctggtc
     9601 aagccgtata ttataattag ttgtagtcat agcaatcacc tttcgcttat attcgtaccc
     9661 ccaatataag ctaaaggtga ctaaacctca ataactttag cattctatct tctcgctaat
     9721 ctaagcggta atagtctgct cattcaatcc agaaagaaac tcgtcaaccg ttttctcaat
     9781 gcttttttgc atcacataaa gatcagaagt ggagtatttg ccaagctgat ttctttcatc
     9841 catgacttca aacagcttgc ttctgaccac ttcttcgtgc gttttatcgt caattaacag
     9901 taaattaagc ttcacgacag ctctattgta ggcttcttga gtggcttcaa gttgaaggct
     9961 tttactgcta gctactgata caccgaacat gctcataaat aactccgcta ctagaatcat
    10021 ctgtagctac agatgatttt tttttaggtg aataaattat tgataccagc ctaagattat
    10081 gccagtgaat gactggaagt aacgctctta taagcccttt tttgtagcag attttacgac
    10141 agataaaaaa atccgttatt tcccacctaa tttaatgact acaccttacg caccctaaag
    10201 ggactgccaa atagctgata ttgttaggtt tattaaaatt tgagtgtttg caaagtgcaa
    10261 acaaaatcat tataagttat tgatttaaaa agggaaaaag gtgtttacaa tctgtaaaca
    10321 cctaagttga aaaaacagtg aaaaacagcc ccctttttac cgccttttgc agcaaaaatc
    10381 tcgctttcct aacaggtgaa tttatggtgt agtaggtaca ctttagtaaa ataacaccca
    10441 attatcagga aaaagtagta atgaactatg cgattttgag gacgacaaaa ttaaagacga
    10501 tgggcaacat tggtggcagt cttgcacaca gctatcgaac gatagaaacg ccaaatgctg
    10561 accccaacct caccccctaa aaatcatcat agcgttgcta cccctgaagg ggttaaacaa
    10621 gcaataaaag attgtctccc tgaaaaaaga cgctctgatg cggtgctctg tattgagtat
    10681 ttaataaccg ccagtcccga atgggaaggc tgggggaaaa agccaagaag tagaattttt
    10741 taagcggtct gctcagtggc tcattgatca gcatggggaa gaaaacattg caggtatgag
    10801 tattcatcga gacataagca caccgcatct agtcgcttat gtcgtaccta ttgaccaaaa
    10861 aggggaagct caactgtaag aattttctag gcggtagggg ctaaattaaa caagatgcaa
    10921 accgattttg ctaataaggt cgctgatcta gggctaatga ggggcaaaga aggctctaaa
    10981 gccaagcata ccagcatcaa ggaatattac cacgacatca accatgctag agattttagt
    11041 attacaaccg tatcacttaa gcctgaaatg tttgaaagca aggctagata tgggggaaaa
    11101 ggtcacagca gccgttattg agcaaataga accgacggta aaagcggcaa acagcatccc
    11161 taatggacta tgagaaagct agattggata aaagcgttgc ggaagcgagc tataactctc
    11221 tcaaaaaaac gtgttgagcc ttacttactg gcgactgaag ggttaaatcg agaggagatc
    11281 attagattga atgaggcaat gcagatagaa agctgtagaa tagctgtaga gagtgaaaaa
    11341 atttaaaata gctatacatg tataactata gagatagtta gcctttaaat cgaaatctgt
    11401 gaacaaatag agtggttttt atatctgatc actaaggttt aagaaaaaat acagtcatta
    11461 gaatactgaa aaacttaaaa agttcaacaa acctgctgtt ttgcattaag ttaaaacaaa
    11521 ttgatggata agtttgaaag tattaatcga ttatctgatc caaataagca taaaaaagtc
    11581 cctgatattt tgaggtaact aaggcttttc
//
