LOCUS       NZ_CP116172             7559 bp    DNA     circular CON 25-FEB-2024
DEFINITION  Escherichia coli strain DETEC-P1056 plasmid pDETEC83, complete
            sequence.
ACCESSION   NZ_CP116172
VERSION     NZ_CP116172.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31988445
            Assembly: GCF_027944915.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7559)
  AUTHORS   Moran,R.A., Baomo,L., Doughty,E.L., Guo,Y., Ba,X., van Schaik,W.,
            Zhuo,C. and McNally,A.
  TITLE     Extended-spectrum beta-lactamase genes traverse the Escherichia coli
            populations of ICU patients, staff and environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7559)
  AUTHORS   Moran,R.A., Baomo,L., Doughty,E.L., Guo,Y., Ba,X., van Schaik,W.,
            Zhuo,C. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2023) Institute of Microbiology and Infection,
            University of Birmingham, Institute of Microbiology and Infection,
            Birmingham B15 2TT, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/25/2024 01:49:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,863
            CDSs (total)                      :: 4,743
            Genes (coding)                    :: 4,559
            CDSs (with protein)               :: 4,559
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 64 of 184
            Pseudo Genes (incomplete)         :: 109 of 184
            Pseudo Genes (internal stop)      :: 47 of 184
            Pseudo Genes (multiple problems)  :: 37 of 184
            Pseudo Genes (short protein)      :: 3 of 184
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116172.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="DETEC-P1056"
                     /isolation_source="ICU patient rectal swab"
                     /db_xref="taxon:562"
                     /plasmid="pDETEC83"
                     /geo_loc_name="China: Guangzhou"
                     /collection_date="2019-10-22"
     gene            254..448
                     /locus_tag="PIC86_RS24185"
                     /old_locus_tag="PIC86_24180"
     CDS             254..448
                     /locus_tag="PIC86_RS24185"
                     /old_locus_tag="PIC86_24180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041629.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHELA
                     EELGNSIQTHFEAQYGTEP"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1097..1240
                     /locus_tag="PIC86_RS24345"
     CDS             1097..1240
                     /locus_tag="PIC86_RS24345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_152896351.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     gene            complement(1435..1566)
                     /locus_tag="PIC86_RS24190"
                     /old_locus_tag="PIC86_24185"
     CDS             complement(1435..1566)
                     /locus_tag="PIC86_RS24190"
                     /old_locus_tag="PIC86_24185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            1596..1886
                     /gene="cki"
                     /locus_tag="PIC86_RS24195"
                     /old_locus_tag="PIC86_24190"
     CDS             1596..1886
                     /gene="cki"
                     /locus_tag="PIC86_RS24195"
                     /old_locus_tag="PIC86_24190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            complement(1883..3529)
                     /gene="cka"
                     /locus_tag="PIC86_RS24200"
                     /old_locus_tag="PIC86_24195"
     CDS             complement(1883..3529)
                     /gene="cka"
                     /locus_tag="PIC86_RS24200"
                     /old_locus_tag="PIC86_24195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_011264160.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            3770..4060
                     /locus_tag="PIC86_RS24205"
                     /old_locus_tag="PIC86_24200"
     CDS             3770..4060
                     /locus_tag="PIC86_RS24205"
                     /old_locus_tag="PIC86_24200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094315653.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTSPSA"
                     /name="hypothetical protein"
     gene            complement(4052..4330)
                     /locus_tag="PIC86_RS24210"
                     /old_locus_tag="PIC86_24205"
     CDS             complement(4052..4330)
                     /locus_tag="PIC86_RS24210"
                     /old_locus_tag="PIC86_24205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094315650.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQEAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            4452..4871
                     /locus_tag="PIC86_RS24215"
                     /old_locus_tag="PIC86_24210"
     CDS             4452..4871
                     /locus_tag="PIC86_RS24215"
                     /old_locus_tag="PIC86_24210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_094315649.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(7001..7195)
                     /locus_tag="PIC86_RS24220"
                     /old_locus_tag="PIC86_24215"
     CDS             complement(7001..7195)
                     /locus_tag="PIC86_RS24220"
                     /old_locus_tag="PIC86_24215"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="WP_116835902.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWNDALHYAKG
                     ENIGWCIARAEYCRKTGRF"
                     /name="ANR family transcriptional regulator"
     Typing          complement(529..610)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.795"
                     /coverage="93.0"
                     /evalue="6.91e-34"
     Typing          6115..6193
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.5"
                     /coverage="87.0"
                     /evalue="1.57e-30"
     Typing          627..1169
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.157"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcgtcacttt gtcaaaacgt caaagtgacg cacatcagga accggcggaa cgttcatgtt
       61 tatgacgaca actgcacaca acgcagacag aaaatcgcgc acatattcag caaaaaataa
      121 gcgtcacggg gtgttaatgt cgcgcagccc acacacgacg gggcttgtgt gataattttc
      181 agtgacgtca cctggcgtga tgaccaggtg acgcacagcc ggacgtaaac accagtaacg
      241 gagaagggag aacgtgaaca aacagcagca gaccgcgctc aacatggcgc gatttatcaa
      301 aagccagagc ctgacgttgc tcgaaaaact ggatgcactg gatgcagacg aacaggccgc
      361 catgtgcgaa cgactgcacg aactggcgga agaactcggt aacagcatac agacacactt
      421 tgaagcgcag tacggcacgg aaccataaag gggctggcga aagcggtcgc ggcttgtccg
      481 tgagtgtttt cgccagcaga caagctcgtc agggcgcatc agcgggtttt agcgggtgtc
      541 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaatcatat
      601 ggcatcagtg cgggttgtat gaaaagtgca ccatgccggg tgtgaaatgc cgcacagatg
      661 cgtaaggaga aaatgctcgt ccaggcgctt ttccgcttcc tcgctcactg actcgctacg
      721 ctcggtcgtt cggctgcggc gagcggtatc tgcccactca aaagcagtga tagtgttatc
      781 cacagaatca ggggatgtaa gcaagaaacg aaaaccccga aaaaggccac gccggaggcg
      841 ctttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      901 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccccggaa gctccctcgt
      961 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1021 aagcgtggcg ctttctcata gctcacgctg ttggtatctc agttcggtgt aggtcgttcg
     1081 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1141 taactatcgt cttgagtcca acccggtaag acacgactta acgccactgg cagcagccat
     1201 tggtaactgg atagtggatt tagatacgca gaactcttga agttgaagcc ttatagcggc
     1261 tacactggaa ggacagcatt tggtatctgt gctccactaa agccagttac ccggttaagc
     1321 agtccccaac tgacttaacc ttcgactaaa ccgcctcccc aggcggtttt ttcgtttaca
     1381 ggcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctactg
     1441 aaccgcgatc cccgtcagtt cagaagacga agatggtgca acggttcctc cttgtacatc
     1501 acgaatatag tttgcttgac atcctgacaa gaataatatt gtaattagaa aaattattat
     1561 tttcatattt ctttccaaca aaagtaaaaa tacttatgca tttaaaatac tacctacata
     1621 atttacctga atcacttata ccatggattc ttattttaat atttaacgac aatgataaca
     1681 ctcctttgtt atttatattt atatcatcaa tacatgtatt gctatatcca tactctaaat
     1741 taaccatatc tagatatatc aaagaaaata caaagttaaa aaaagaaccc tggtacttat
     1801 gcaagttatc tgcattgttt tatttattaa tggcaatccc agtaggattg ccaagtttca
     1861 tatattacac tctaaagaga aattaaatcc ctaataactc attaagtttg ctcaactcat
     1921 catcccctat gtaagaagaa acaatacctg tgacaattgc aatcccccag aaaccaagag
     1981 gcacaccaac aataaaacta aacattaatg ccacaatttt tgccacacct acatcgaccg
     2041 cacttttctc cagcgtgaca aacaaaggtc gccagttacc agtttctatt gcatttttaa
     2101 aatcagaacc aacatcatat agaaaagata ctcgactggt gattttaagc gactttgata
     2161 tcttcgttaa attcttagat aactcatcat aattaacaga ctctaacgca ttaaaaatag
     2221 catcccgatc aaccttactg aatttcttat ccagcacatt cttatacttt tcaaatgctg
     2281 ccagagcttc atcaacacct tgaattttct ttcctttaga cttttccgct aaatcctgag
     2341 caatttttgc gtatttttct ccatattgtt cggttatata ttgataaaat ccaaccatag
     2401 tatcaacagc atcttttatc tggctctttt caagagcatc ctgagcttct tttaatttct
     2461 gctctgcctc tttaacctca gcggccttac catccctgaa actaacagca ttcttaagtt
     2521 cattttcaac tgcggagtac tcagcctgct tagcttcaag ctgacgctgt agttttttct
     2581 gaacgtcacg ccatcccgga aatccggtga ccttcacgtc aacactcttc tgagcgtttt
     2641 caagctctcc tgcaactctg gaaacagtag cttgcttact ctgtacatca ctttcagcct
     2701 tcgccagttc agctttcgcc tcagctaaac gcttctctgc ttccgccacc gcctgtttct
     2761 cttcattaag agtttcatgt tcagcgacct ctgcattttc acgagccttt tcttcagctt
     2821 ccttctgttt attcacatca ccaattttac tttgcaatgt atttttataa gaattaagtt
     2881 tgctaatatc tgcatcaagc tgatttgatt ttttttgcaa ctcatcaaca tccctctcaa
     2941 gatcagtgat accatgataa gaatgatgct tgaatacttt tttcatctcc tcgattttct
     3001 tctgtttttc actaatctgt gtagcaattt tattcttctg cttttgtttt tcattaatta
     3061 cattacccac caccttcgaa ctcttatcca tatcactgac ctgagcattc gttggtgcag
     3121 tattaactga agcagtgtta ttgttgttat ttcctgagct ttctgcaaaa agtgatggca
     3181 tatcactaat taaagaatta agaactctgg agacccctcc aaatggatta tcaaccagag
     3241 ttgaattctc ttctgtcata acaataccat tcatcaccgg aaggccatca ttattaatga
     3301 caacatcacc ccacggagtc agatatgact caccggtttt catcacagtt gatgtagaac
     3361 cagatgaatt tgaatttccc tgaccaccat tattaccatg cccagagcca ccaccccagt
     3421 gaaccccact attactatta ttatttccac cttgctgatt cagattcgcc cctgttcccc
     3481 ccatagactc accagcagtt ggtccatatc cacttagttc tttagccata aattcctctt
     3541 tgataattaa aacaataaat taaaaacaac gtactgtaca tataaccact ggttttatgt
     3601 acagtggaaa cctactactc agcattgccc atgtcaagaa catggatttt catttttgca
     3661 ataaagatca cactacaagg aggcagacat tgagaacgtc gaaacagaac accggagcaa
     3721 atcaggatga gatataaaac tgttggatca tgaaaaaacg gagaacgatg tgagcaaatc
     3781 tccccgccat aaactgaaca aaacagacaa gcgacttctc gacaccctgg ttgctgccgg
     3841 atatgagcat gacaaagccc gtgacctcat ccagaaacag gtttacacgc tgacactggc
     3901 agatcagcgt catgtggtca gtgaaatcag taatggtgtg aatcccaccc aggcttactc
     3961 ggcggtatac caggcaagac gcattcgcct cgcccgtaaa tatctgaacg gtaaaaaggt
     4021 tatggaagaa accggggaaa atacgtcccc atcagcgtaa ggatttcttt tgccgctcca
     4081 gagactccag ttttttacgc aaatcctctc ttttttgggc atctctggtg ccagccagct
     4141 ctgctctcaa ctcatcgatc tgaagttgta tcttcagtct gttactgaac attttctgtc
     4201 tggcattaac atccgcaaca atgccgtttt ttgtcttctc ggcctcttgt tgaaaaacat
     4261 tgctgtccgc atgactggca accgaagcag aaagaacact aaaaagcagg actggcacac
     4321 attttttcac gggattattc ctgactcatt gaccatcaaa tcacattggg agtaaaccga
     4381 cgtatgataa gagatactct tcggagatat aactccctga gtatcaagat taaaaacgca
     4441 aggagatgtt tatgagatct gccgctgcca ggctgcttct gatacctctg ataacagcaa
     4501 caatagctct tacaggatgc acaccaaaga ccagcctgga acgacatacc cggcattatg
     4561 tttatgcttc agatgatgga tttgatccta acttctacac ccagaaagca gacaccatac
     4621 gtatgatggt gccttttttt cggcagttct gggatatggg agctaaagat aaagcgacag
     4681 gaaaatcacg gagtgatgtg caacaacgca ttcagcagtt tcacagccag gaatttttaa
     4741 actcactccg gggcacaact caatttgcgg gtactgatta ccgcagcaaa gaccttaccc
     4801 cgaaaaaatc cagactaatg gcagacacca tttctgcggt ttatctcgat ggatacgagg
     4861 gcagacagta agggatttac cataatccct taattgtacg caccgttaaa acgcgctcag
     4921 cgcgtttctg gcgcaaaaac cgtaaaaagg gatgttttcc ccggggtaaa caggaaaaat
     4981 gcgtcaggaa cgttttcagc gcgttgcatg actatgcatg aaactgaatg gcgatcggtt
     5041 tgggcgcgtc cgctgtccct gaggcttatt ttcgtaaatt ttcagttcgt aattctctga
     5101 ctcattgctg catatcagac cagaacgatc ctaggagcgc ccctaggatc gctctgtaaa
     5161 aaaatgcgac acgcccctag ggccgcccct acattaatta actgtttttt tctacagata
     5221 gtcagtactg accctaagca gggtcgtcat agcgtcgcca tagggtcgtc gacgacgtgc
     5281 ttttgtataa atctgaataa gaaaatatat ttttagagcc ataacgacat gatttaaaaa
     5341 gcaaatcagc aaaaagttgg ttttgcgtag tgtgtgggca tttcaggcat gagaacagac
     5401 tcgcgttttt ccggaagaac tgcggggatt tttgattaaa caatactcac cgcagagcgg
     5461 aattttatgc aacgccggct gtgcggcaca gggattttta atccccagta tgtcagaggg
     5521 tgcggatgac ggttcccgtc tggcgttttc atgggcgccg gttgtcaaaa aatgccagcg
     5581 tgatcggtcg aaggataaac gcagattaat cagtgatgat tgagcgcgaa tctgacaacg
     5641 agtatagcga cacagcaaca aaaaagacgc cagaaacgcg caggagtgtt ttagcgtgtc
     5701 tccggcgtga tgtcgagttg tgcggtaatg agtgagcgaa aaaaatcccc tggattacag
     5761 ggggaaccca ttatcagact ttgaagttta tttgttattt gatggagtga gcacgaatcc
     5821 cagagatgac gttcaggtgg caaggaaacg cgctcagacg ctttctaacg cacttttttg
     5881 catcgggcaa ttattttata tgttttggtg ggttcaggct gtcagagacg ttttgagcgc
     5941 gtttctggcg cttgctgcta ctgccaaacc atccccatgg ccagttgatg ccagaaacgc
     6001 gcaggagttt tgtagcgcgt ctccggtccc tgatcaatga attcccggtc attaacatgc
     6061 gtttacccgg aaacgcgccc tgatgttcta tcgtatcgcg ggaacggtgt cgagggtgtc
     6121 ggggcaaagc cctgaccagg tggtaatcgc atagccgtgc gtgcgcggtt atacgattac
     6181 acatcctgtc ccggaatcag gattgttttt cgcgcacaac tgcggcaaat tcaccccgtt
     6241 gcggttgcaa cgatgggtat atcaaaacta cgaaaattat tcaggcattt gctacccccc
     6301 ttgccctggc ctccaccagt cacgggggat ttttttgttg tctctcatga cgaactacgg
     6361 caaatttctg acgttgcaaa ctgcaacaca taaaacacac acaaaactca caattgccat
     6421 gtcctcattg acaccccctt gccatatagg gggatttttt cgcctgtcct gattgtcttt
     6481 tgccgatggt tacggcagat tggaaaagag cgcatttgtg gtgctctctc cagaataata
     6541 tttctcatta ctcaaactct gatggcttcc ccctgtaatc cagggggatt ttttaattta
     6601 tcgctatgct tcgtaattca ttcagaatac agacagtatg gttaatcagt ttttcatcat
     6661 ttttgcagtg tttgccctgt cctgcatcag aacggtaaat acaggaatct ctttatgacg
     6721 aatcctccat attcaggatt ttttatgtag cgtctactac atgcgatccg ttggtaaacc
     6781 gttagttata tattaattat atcttaatta tatacacacc aggttagtta taccttggtt
     6841 atatgttaat tatatcttgg ttatactttt gttatgcgtc agttacagag gcgttttgag
     6901 ggtaaattct ggaggcacgt gaccgactgt atacaaaatg ctggcgcatt ttgatacgtc
     6961 gacgtgccaa aggggactcc cctttgaaac cccgccagaa ttaaaaccgg cctgtctttc
     7021 tacaatattc agctctggct atacaccaac ctatattttc tcctttggca taatgaagcg
     7081 catcattcca ttttttccat gctccaatgt aatcgccttc acgctccagt ctgacagctt
     7141 cgtttgcggg tgaaaaatat ttattttcta aataaattgc tttgtcctga ttcatactcg
     7201 tatttatatt tttattggaa ttaaaaagag cggcctgtcg ggttaataca tattggcatg
     7261 actgttgata tatcagccca actgcacgct cgtaatcgtt ttaacgtgat tttcgcaata
     7321 tggcaatccg ggttcgtctg tccggtaatc aataaaaacc gtgtgaaaaa cgggacagga
     7381 tgtgcatttg tcggaccgcg cacacacgac cggacaaacg ccacccggtc atggctgcgc
     7441 cccgacaccc cgcgaacgtc tctggccaga actggccatc acgccgggtc acgttgtcag
     7501 atgtaaaaaa atcacgcggt aaacgccgtt attcgcgggt ttgcgcaagc acctcccgt
//
