LOCUS       NZ_CP116119             8520 bp    DNA     circular CON 25-FEB-2024
DEFINITION  Escherichia coli strain DETEC-P793 plasmid pDETEC16, complete
            sequence.
ACCESSION   NZ_CP116119
VERSION     NZ_CP116119.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN31988477
            Assembly: GCF_027944775.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8520)
  AUTHORS   Moran,R.A., Baomo,L., Doughty,E.L., Guo,Y., Ba,X., van Schaik,W.,
            Zhuo,C. and McNally,A.
  TITLE     Extended-spectrum beta-lactamase genes traverse the Escherichia coli
            populations of ICU patients, staff and environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8520)
  AUTHORS   Moran,R.A., Baomo,L., Doughty,E.L., Guo,Y., Ba,X., van Schaik,W.,
            Zhuo,C. and McNally,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-JAN-2023) Institute of Microbiology and Infection,
            University of Birmingham, Institute of Microbiology and Infection,
            Birmingham B15 2TT, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 10x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/25/2024 01:55:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,351
            CDSs (total)                      :: 5,234
            Genes (coding)                    :: 4,997
            CDSs (with protein)               :: 4,997
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 84 of 237
            Pseudo Genes (incomplete)         :: 166 of 237
            Pseudo Genes (internal stop)      :: 38 of 237
            Pseudo Genes (multiple problems)  :: 48 of 237
            Pseudo Genes (short protein)      :: 4 of 237
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP116119.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="DETEC-P793"
                     /isolation_source="ICU patient rectal swab"
                     /db_xref="taxon:562"
                     /plasmid="pDETEC16"
                     /geo_loc_name="China: Guangzhou"
                     /collection_date="2019-09-12"
     gene            97..1011
                     /locus_tag="PIC92_RS26585"
                     /old_locus_tag="PIC92_26590"
     CDS             97..1011
                     /locus_tag="PIC92_RS26585"
                     /old_locus_tag="PIC92_26590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271363.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(1215..1613)
                     /locus_tag="PIC92_RS26590"
                     /old_locus_tag="PIC92_26595"
     CDS             complement(1215..1613)
                     /locus_tag="PIC92_RS26590"
                     /old_locus_tag="PIC92_26595"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            1790..2716
                     /locus_tag="PIC92_RS26595"
                     /old_locus_tag="PIC92_26600"
     CDS             1790..2716
                     /locus_tag="PIC92_RS26595"
                     /old_locus_tag="PIC92_26600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            2719..3252
                     /locus_tag="PIC92_RS26600"
                     /old_locus_tag="PIC92_26605"
     CDS             2719..3252
                     /locus_tag="PIC92_RS26600"
                     /old_locus_tag="PIC92_26605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(3318..3527)
                     /locus_tag="PIC92_RS26605"
                     /old_locus_tag="PIC92_26610"
     CDS             complement(3318..3527)
                     /locus_tag="PIC92_RS26605"
                     /old_locus_tag="PIC92_26610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            4183..5445
                     /locus_tag="PIC92_RS26610"
                     /old_locus_tag="PIC92_26615"
     CDS             4183..5445
                     /locus_tag="PIC92_RS26610"
                     /old_locus_tag="PIC92_26615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISEcp1 family transposase"
                     /protein_id="WP_000608644.1"
                     /translation="MINKIDFKAKNLTSNAGLFLLLENAKSNGIFDFIENDLVFDNDST
                     NKIKMNHIKTMLCGHFIGIDKLERLKLLQNDPLVNEFDISVKEPETVSRFLGNFNFKTT
                     QMFRDINFKVFKKLLTKSKLTSITIDIDSSVINVEGHQEGASKGYNPKKLGNRCYNIQF
                     AFCDELKAYVTGFVRSGNTYTANGAAEMIKEIVANIKSDDLEILFRMDSGYFDEKIIET
                     IESLGCKYLIKAKSYSTLTSQATNSSIVFVKGEEGRETTELYTKLVKWEKDRRFVVSRV
                     LKPEKERAQLSLLEGSEYDYFFFVTNTTLLSEKVVIYYEKRGNAENYIKEAKYDMAVGH
                     LLLKSFWANEAVFQMMMLSYNLFLLFKFDSLDSSEYRQQIKTFRLKYVFLAAKIIKTAR
                     YVIMKLSENYPYKGVYEKCLV"
                     /name="IS1380-like element ISEcp1 family transposase"
     gene            5701..6576
                     /locus_tag="PIC92_RS26615"
                     /old_locus_tag="PIC92_26620"
     CDS             5701..6576
                     /locus_tag="PIC92_RS26615"
                     /old_locus_tag="PIC92_26620"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011091028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class A beta-lactamase
                     CTX-M-55"
                     /protein_id="WP_015387340.1"
                     /translation="MVKKSLRQFTLMATATVTLLLGSVPLYAQTADVQQKLAELERQSG
                     GRLGVALINTADNSQILYRADERFAMCSTSKVMAVAAVLKKSESEPNLLNQRVEIKKSD
                     LVNYNPIAEKHVNGTMSLAELSAAALQYSDNVAMNKLIAHVGGPASVTAFARQLGDETF
                     RLDRTEPTLNTAIPGDPRDTTSPRAMAQTLRNLTLGKALGDSQRAQLVTWMKGNTTGAA
                     SIQAGLPASWVVGDKTGSGGYGTTNDIAVIWPKDRAPLILVTYFTQPQPKAESRRDVLA
                     SAAKIVTDGL"
                     /name="extended-spectrum class A beta-lactamase CTX-M-55"
     gene            complement(6623..>6841)
                     /locus_tag="PIC92_RS26620"
                     /old_locus_tag="PIC92_26625"
                     /pseudo=""
     CDS             complement(6623..>6841)
                     /locus_tag="PIC92_RS26620"
                     /old_locus_tag="PIC92_26625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243817.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="cupin fold metalloprotein, WbuC family"
                     /name="cupin fold metalloprotein, WbuC family"
     gene            complement(<6838..>7347)
                     /locus_tag="PIC92_RS26625"
                     /old_locus_tag="PIC92_26630"
                     /pseudo=""
     CDS             complement(<6838..>7347)
                     /locus_tag="PIC92_RS26625"
                     /old_locus_tag="PIC92_26630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA]"
                     /GO_process="GO:0009297 - pilus assembly [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /name="fimbria/pilus outer membrane usher protein"
     ARG             5702..6573
                     /name="blaCTX-M-55"
                     /gene="blaCTX-M-55"
                     /gene_name="extended-spectrum class A beta-lactamase
                     CTX-M-55"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          98..1011
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.909"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1791..2713
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaaacaaac gcccctgaaa ttcgtgtcta tccgccaaga ctttcacgaa aatacagggg
       61 cgatgtcggt cgataacctg gaaggaatcg taacacatga gcgccgcgct tcaatacttc
      121 gaagaaaatt taccccaccg tccctatcac acggatgatc tcgctttcgg ccttcgtatc
      181 tccggcaaag ggcgcgcgct tcttgcacgg tatatccagc agaaccagcc tcatgcacag
      241 ttctggctgg tttttgacgt tgaccgcgag ggggctgcga ttgactggag cgaccggaac
      301 gctcccgcgc cgaacatcac cgttaaaaat cctgtgaacg gacacgctca cctgctctac
      361 gcactcaaca tcgccgtgag aaccgcgcct gatgcctcgg ttaaggcgct gaaatatgcc
      421 gcagcggttg aacgttcgct gtgtgagaaa ctttgtgcgg atgtgaacta cagcggcctg
      481 atttgcaaaa atccgttcca cctggaatgg ctggtgatgg agtggcgcga ggaagcctat
      541 accctcgatg aactggctga ttatctcgat ttgagcgcct cagagcgtcg tagcatcgat
      601 aaacattacg ggatggggcg aaactgccac ctgttcgaaa tgacgcgcaa atgggcttac
      661 agggcgattc gtcagggctg gccagcgttc tcacagtggc ttgaagcggt gatccagcgt
      721 gtcgaaatgt acaatgcatc gcttcccgtt ccgctttcac tggctgaatg tcgggctatt
      781 ggcaagagca ttgcgaaata cacgcacagg aacttcacgc cggaaacttt cgcacagtat
      841 gtggctgata cgcacacgcc agaaattcag gctgcacgcg gtcgcaaggg cggttctaag
      901 tctaagcgca gcacagtagc tacatctgca cgcacgctga aaccgtggga ggcattaggg
      961 atcagtcgcg cctggtatta ccaactgaaa aaacgaggtc tcgtagagta gaccaaataa
     1021 gcctatatca gataacagcg cctttctggc gtctttttga gcagcaggtt ttttgccgtt
     1081 tttccccgtt cagccctgca atggcgcggc tttccgcgtg attgtggtgg tagtgctcgc
     1141 cgcagtctca tgaccgagcg tagcgagcga atgagcgagg aagcaccagg gaatcggaac
     1201 acggatggag gcacttacgc gtctgggttt ggggatgtct ccagatcgta cttaagttcc
     1261 ttaagtgctt gtaatatcct gtctttttta tagcatagct cgcctgtttt tgtgtctacc
     1321 agtcctgctt cgatcagtag cccagccgcg agaaagagcc ggtgattgcg ttcccgtcgt
     1381 tgtttttttc cctcgattcg tttttggacc gcctgttttc gttcgctctc ctgtgcctgt
     1441 aagtcgtttt tttccgctgt tgccagtacg tggagaagtt tgcgttcctg ttctgtgagt
     1501 ccggcttcgt tgtcgatcag gtcaataatc tgtctctgtc gtgcggtctg ttctccgagc
     1561 ttcctgatga atccgatccg tttcttcagc cattcgatgt tgctgatgcg catgttcgtg
     1621 atgctccctg gtgttttttt gcggtgtgtt gtggtcgtgc agtatatcgc ttgcggtcgg
     1681 cgggtgcagg gggtatcttc cgtgctaatt cgtaaaatta gaaggaagcg cacttatatg
     1741 tttctctcct tcgtcgagaa acagtgcgct cctgcagagg gctgaaaccg tggcaaccta
     1801 tcacctcagc gttaaatttg gtggcaaggg gcaggctgca aaccacgccg attacatcga
     1861 gcgcaaagag aaataccgcg accgtcagga tttggaatat tccgcacacg gaaacatgcc
     1921 ggaatgggcc agggataatc cttcccattt ctggcaggcg gctgaccagt ttgaacgtgc
     1981 gaacggttcg acctataggg aacttgaaat tgcattaccc cgtgaactga caccggaaca
     2041 gcgccttgaa ctggtgcagg attttgtacg tcaggaagct ggcgaacggc acgcctggag
     2101 ttttgccatt cacaatccga aagcgagcat tgacggcggc gaacagcctc atgcgcatat
     2161 catgatgagt cagcgtgtga atgatggcat tgaacgtaca ccggaacagt atttccggcg
     2221 ctataacgcc aggtatcccg agcggggagg tgcgaaaaag gattccggaa gcctgactct
     2281 cactcagcaa aaagagcagt tgcgggaatt gcgaaaacgc tgggaggtga agcacaacga
     2341 acatatgaga aagcatggct ttgaaagggg cttcattgac tgccggacgc tgaaagaaca
     2401 gggcattgaa cgacgtcccg aagtgcatct gggttttgaa gctgccggac ggcttgatga
     2461 tgcccagcgt catgacatca tgcaaaaacg gtctgaaccg gactattccc gccatcttga
     2521 acaggcgcga acggtcgggc atgacatcag ggaatggctg gacactccgg ttaagcaaca
     2581 tgaactggtc agaaggctgc aacatgatgt tgcgcgggag catggctttg acatgagaga
     2641 cagagtgagc agacagaacc gaactgcacc tgaacgggtt aaatcaccgt cacgtggatt
     2701 ttctatggga ttctgaacat ggccagcaca gaagaaaaat tactcacgct cctgttatcg
     2761 gtggagcatc tgcaacatac cgcaatggag caacagaaag ctgttgctga ttctctggct
     2821 gtggcaaggg aaactcttcg cggatatcgg gatatttctg acggtgtggc aaaacaggtc
     2881 agggatgaag tcagaaatga ggtgcagaag ctggacgtgg caggactgat aggtgagcgc
     2941 atcagccagg attttcagcg gctggaagag tcagtcgcaa acatgaaggc aaacgcggat
     3001 gccctgaaca gggatattgc gacaattcac agcaatatcg tgcaggaata caacaccctt
     3061 aaagggtata gctggaaatg gctgggtggg gtgtcagtgg cttttctggt tatgttctgt
     3121 gtgctcggat ggggaatgaa aacaatactc gacaggaact acaacgccac agcggcggtg
     3181 tatcagaaag ttgtgacact ggaagataaa ctggtggaga aacctgtcac ggggaagagc
     3241 cggaaaaact gacgcgcttc gcttgtccaa cgcctcacga cccgcgaaag gatttcgcga
     3301 accgttcacc gtttttttca atatgattct gtctgctttg gtcgataaag cagacgatag
     3361 tattccagcc ttcgtatata taacggtacg tgctcatcag taagagctgg cggtattgtg
     3421 ttatcagggg gaagtttttt cagatactca cgaaatgcct ggcttgatgc cataaaatct
     3481 acagtagcat cgataatatg tggggtgttt tcacctaatt ttgccatgat gataatcctt
     3541 atattatcac ggtttgtgaa tgatagggtg tcactattat ttatgcgtta gtgaaccaga
     3601 aaaaatcccc cgttggcata gggggatatg taaggagaaa taacttcgca ggtgttcttg
     3661 tttgtataac gtgaaaagac tgcacgcaac ttaataagtc tgccgtactt actttcagtt
     3721 ttcaacataa ggtttataac aatgtatgga atggaagtga gctttaaggt ttcagctcta
     3781 aaaaataaaa tatttattag ttggctggtt gttgttcttg ttctgtatgt atataaacac
     3841 tatcgacata ttatgaccgg gaacattcct taccccctct gcgctcgcag aggttttttt
     3901 tgtcattaat taataatgaa ctgaaatgca gttccgatac ctccatttgc acattatccc
     3961 tgacatatgg taccggaacc gcattaattt tatgttccta gattctacgt cagtacttca
     4021 aaaagcataa tcaaagcctt gataaatatg cattccttcg aaattcagct ttcacccatt
     4081 gggtgaaaga aaagtgctca aaaatatgtt aaattatcag cttttatgac tcgatatatg
     4141 gtaaaataat agtaagaaaa gtagtaaaaa ggggttctaa ttatgattaa taaaattgat
     4201 ttcaaagcta agaatctaac atcaaatgca ggtctttttc tgctccttga gaatgcaaaa
     4261 agcaatggga tttttgattt tattgaaaat gacctcgtat ttgataatga ctcaacaaat
     4321 aaaatcaaga tgaatcatat aaagaccatg ctctgcggtc acttcattgg cattgataag
     4381 ttagaacgtc taaagctact tcaaaatgat cccctcgtca acgagtttga tatttccgta
     4441 aaagaacctg aaacagtgtc acggtttcta ggaaacttca acttcaagac aacccaaatg
     4501 tttagagaca ttaattttaa agtctttaaa aaactgctca ctaaaagtaa attgacatcc
     4561 attacgattg atattgatag tagtgtaatt aacgtagaag gtcatcaaga aggtgcgtca
     4621 aaaggatata atcctaagaa actgggaaac cgatgctaca atatccaatt tgcattttgc
     4681 gacgaattaa aagcatatgt taccggattt gtaagaagtg gcaatactta cactgcaaac
     4741 ggtgctgcgg aaatgatcaa agaaattgtt gctaacatca aatcagacga tttagaaatt
     4801 ttatttcgaa tggatagtgg ctactttgat gaaaaaatta tcgaaacgat agaatctctt
     4861 ggatgcaaat atttaattaa agccaaaagt tattctacac tcacctcaca agcaacgaat
     4921 tcatcaattg tattcgttaa aggagaagaa ggtagagaaa ctacagaact gtatacaaaa
     4981 ttagttaaat gggaaaaaga cagaagattt gtcgtatctc gcgtactgaa accagaaaaa
     5041 gaaagagcac aattatcact tttagaaggt tccgaatacg actacttttt ctttgtaaca
     5101 aatactacct tgctttctga aaaagtagtt atatactatg aaaagcgtgg taatgctgaa
     5161 aactatatca aagaagccaa atacgacatg gcggtgggtc atctcttgct aaagtcattt
     5221 tgggcgaatg aagccgtgtt tcaaatgatg atgctttcat ataacctatt tttgttgttc
     5281 aagtttgatt ccttggactc ttcagaatac agacagcaaa taaagacctt tcgtttgaag
     5341 tatgtatttc ttgcagcaaa aataatcaaa accgcaagat atgtaatcat gaagttgtcg
     5401 gaaaactatc cgtacaaggg agtgtatgaa aaatgtctgg tataataaga atatcatcaa
     5461 taaaattgag tgttgctctg tggataactt gcagagttta ttaagtatca ttgcagcaaa
     5521 gatgaaatca atgatttatc aaaaatgatt gaaaggtggt tgtaaataat gttacaatgt
     5581 gtgagaagca gtctaaattc ttcgtgaaat agtgattttt gaagctaata aaaaacacac
     5641 gtggaattta gggactattc atgttgttgt tatttcgtat cttccagaat aaggaatccc
     5701 atggttaaaa aatcactgcg ccagttcacg ctgatggcga cggcaaccgt cacgctgttg
     5761 ttaggaagtg tgccgctgta tgcgcaaacg gcggacgtac agcaaaaact tgccgaatta
     5821 gagcggcagt cgggaggcag actgggtgtg gcattgatta acacagcaga taattcgcaa
     5881 atactttatc gtgctgatga gcgctttgcg atgtgcagca ccagtaaagt gatggccgtg
     5941 gccgcggtgc tgaagaaaag tgaaagcgaa ccgaatctgt taaatcagcg agttgagatc
     6001 aaaaaatctg accttgttaa ctataatccg attgcggaaa agcacgtcaa tgggacgatg
     6061 tcactggctg agcttagcgc ggccgcgcta cagtacagcg ataacgtggc gatgaataag
     6121 ctgattgctc acgttggcgg cccggctagc gtcaccgcgt tcgcccgaca gctgggagac
     6181 gaaacgttcc gtctcgaccg taccgagccg acgttaaaca ccgccattcc gggcgatccg
     6241 cgtgatacca cttcacctcg ggcaatggcg caaactctgc ggaatctgac gctgggtaaa
     6301 gcattgggcg acagccaacg ggcgcagctg gtgacatgga tgaaaggcaa taccaccggt
     6361 gcagcgagca ttcaggctgg actgcctgct tcctgggttg tgggggataa aaccggcagc
     6421 ggtggctatg gcaccaccaa cgatatcgcg gtgatctggc caaaagatcg tgcgccgctg
     6481 attctggtca cttacttcac ccagcctcaa cctaaggcag aaagccgtcg cgatgtatta
     6541 gcgtcggcgg ctaaaatcgt caccgacggt ttgtaatagc ggaaacggaa tggggaaact
     6601 cattccgttt ttgtttatcg ccttagacgg caaaagtgct gtcgcccacc tgcgcttgcg
     6661 cataccaggc cataagctcc gtggttcctg gttctccttc cgctggagcc cagtgcgcat
     6721 agtcatcggc agccacgggt tgatagccac cgtgttttac ttcaaaaatt atgccaccgg
     6781 tatccagcga cagcacggca tgccaggttc ctgcggccat ctccagcacc gtacaggatt
     6841 caggttatta tccggcagca gagtgccata aacccctgat aggttggtca tgccgccttt
     6901 caaatcgttt gacatactgt aactggcgtt tgaattacga aatgccgact gactgtctgt
     6961 acgcatccaa tgactaaagg gaacattaag cgtaaaagcg agtaaatgat cccgatcgtt
     7021 ttgccatata ttattggaat agctgtaatt cagtgaagtc gtaatatcac caaacggcac
     7081 atttaatcca aatgatattt gctggtcgct gcgtgacgtg ttccagtaac tttggcgact
     7141 ggcactgaaa aatgtcgtac cgtaatttcc aagctgctga gagatgctta tttgttcctg
     7201 accacgctta ctgtagaaca gattaaaata atcaataaat agtgtctgct cactggtgtc
     7261 tccggtagga ggcttgacgg tgtaaccact cattcgactg tatgcactgt cggataagtt
     7321 ataaaaacct tgcgtagaat agcgatatgt tataagagtt tatttttaat gtccagatgg
     7381 tttaatagta tttatatgta attagctgta tggatagtac tggttttatt gtataacttg
     7441 tcgttttttt ttattgtttt tgtgtggtgt gctaatcttt tagttataaa aaaacccctt
     7501 ggaatacagg ggcaaaagta aggaaaacaa atggatgctc agtttgtgtg acactttaaa
     7561 ctaatggaac tatagagtgg aggttgttac agtcgctttt ataaaacagc aaccagtttt
     7621 tttcaatagt ctgatgatgg ttgtgacagt tttgtgatat tgttcaaaat gtttcttgtt
     7681 gaatgtttgt tttttggtct attggtgtct ggttttattt tttctgctat tacatatcaa
     7741 gaaatgtgat tggtgtagca agagtggaat gcgatgtgga taaactaatt ggttgtcgaa
     7801 tctaatttac ttagaaaact acacttgttt ttgtgtatga atttagtgaa tttgtctgtc
     7861 tatgtgtttt cgtgcattta taaatccttc gtcaggatct taaatttagt gttgaatatg
     7921 tagggcctgt acggtaatta ttatttagcg tagctacttc atcgttgttc gaacacaacc
     7981 aacaacctgt aattgttgtt tctcttggtt ccccactttt gtggggtttt ttatctcttc
     8041 cgtgcatcgt aaagcgcgga ctccttctgg aaaggttgtt ttagggcatc actgaggcat
     8101 tttttgcgtt tgtgaatatt gagcatccga taactaaaat atttcccata gactctgatt
     8161 cttgccaccg accccaggtc ggtttttttt tgtcctggcg agatgtgaag tttggaaaca
     8221 ggaaaccccg tcagagcgcc acgcaccagg ggagaaaact tgtcgttttg agccgttcgg
     8281 tgttcagaac gcacgaaacc gatcgcacat cgctttcgtg catagttatg caacgccctg
     8341 aaaacgattc tgacgcgttt ttctggtttg gcctggtgtt ttcttgtctt tttgcgtttt
     8401 ttgcgccaga aagcgactga gggcgtttta aggggtacgt acaacgggag ttatggtaaa
     8461 tggatcggat ttgcgggaag gctcgacagg ttttgtcgtt gggggtagtg taagcgactg
//
